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Gene information
General information
TC013372 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10409698..10411925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013372
    iBeetle screen iB_06314

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    Pupal morphology
    head necrotic - Number of animals showing the phenotype: 3

    eyes necrotic - Number of animals showing the phenotype: 10

    antenna shortened - Number of animals showing the phenotype: 8
    labrum shortened - Number of animals showing the phenotype: 10
    antenna sometimes necrotic - Number of animals showing the phenotype: 4
    elytra elongated - Number of animals showing the phenotype: 10

    dorsal thoracic muscles mostlynot present - Number of animals showing the phenotype: 10

    Comment: No muscle pattern due to necrosis observed in pupa.
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013372 RA
      MEKSPICST EYVFKRKKI APQNIVIRL KNRETFSYR 
      KPGTHFHTA RQFYQCIFP NFTVINVEK PPCFLRKFS 
      PDGKYFIAF SSDQTSLEV YTYRGPAAA ADLFNKTEN 
      CDAEKRSEI SSKIFDRIF KLKYVVHVT QGSEELNRE 
      CSLFSDDGR YVIIGSAAF IPEEIRPHF YEIYTNNES 
      VTPNPRSPL ENYTLHLVD LHQGKLCDS RQFKVDKIF 
      LSHNQGLYL YKDILAVLS VQHQVIHIF QILDGMFVN 
      VRKIGRFCF EDDSYFYDL AYSNERAFR DVSINSLKH 
      RLLTFLYKR AVTISKRDN SPLEIRKFF HHFSNYNAL 
      KLWKMQLLD ENHLLIKYA SEDVVTLKC TDANTQPSF 
      FIVYNMKES KILAIYENT STDLLHLFE NFCDSFRNA 
      CVNCETQFT CSPSNNIYA RILQQRFKQ TIVSAKYGG 
      ETEATKRLL AQLPISAQS YSSSPYLDL SLFSYDDKW 
      VSVMERPKT CGEYPIRFY GRDSGFLKF QIFAGGTRL 
      MYPSARRLV AFTFHPTDP FAISVQRTN ANYVVNFHV 
      RQDAAYANN NFYEDCNFV L
    CDS sequence
    • >TC013372 RA
      atggaaaaa tcgccgatt tgcagcacc gaatatgta 
      ttcaaacgt aagaagatc gcccctcaa aacatcgta 
      atacgtctg aaaaatcgc gagacgttt tcgtaccga 
      aaaccgggg actcacttc cacaccgct cgccaattc 
      taccaatgt attttcccc aattttact gtgataaac 
      gtggaaaag cccccttgt tttttacga aaattcagt 
      cccgatggt aaatatttc attgcgttt tcgtcggat 
      caaacgtcg ctggaagtc tacacttac agagggccg 
      gcagcggcg gccgaccta ttcaacaaa actgagaac 
      tgtgatgcc gaaaaacgg tcagagatt agtagtaaa 
      atttttgat aggattttc aagttaaaa tatgtggta 
      catgtgacc caaggctct gaggaactt aatcgcgag 
      tgtagcctt ttcagtgat gacggcaga tacgtgata 
      atcggttca gcggcgttc ataccggaa gagatacgg 
      ccacacttt tatgaaatt tatacaaat aacgaatcg 
      gtcacgcct aacccgcgt tcccctttg gaaaattac 
      actcttcat ttggttgac ctccatcaa ggaaaactg 
      tgtgattcg aggcaattt aaagtcgac aaaattttc 
      ctgtcgcat aaccaaggc ctctattta tacaaggac 
      atactcgct gtgctctca gtccaacac caagtgatc 
      cacattttc caaatcctg gacggaatg ttcgtaaat 
      gtccgaaaa atcggccga ttttgcttc gaggatgac 
      tcgtatttc tacgacttg gcgtattcg aacgagagg 
      gccttcagg gacgtgagc ataaactcg ctaaagcac 
      cgattgcta acgtttttg tacaaaaga gcagtaacc 
      ataagtaaa agagacaac agtccccta gaaatccgc 
      aaatttttc caccatttc agcaactac aacgcactg 
      aaactgtgg aaaatgcag cttttggat gagaaccac 
      ttgttgatt aagtacgcg agcgaggac gtcgtcacg 
      ttaaagtgc accgacgcg aacacccag ccgtccttc 
      ttcatagtt tacaacatg aaggagagc aaaattttg 
      gcaatttat gaaaacaca tcgactgat ttgttgcac 
      ttgtttgag aatttctgt gattcgttt aggaacgcg 
      tgcgttaac tgcgagacg cagtttact tgtagcccc 
      tctaataac atttacgcc aggattttg caacaaagg 
      ttcaaacaa accatagtc agtgcaaaa tacggtggc 
      gaaacggaa gccactaaa agactccta gctcagtta 
      ccaataagt gcccaatct tacagcagt tcgccttat 
      ctggacttg tcgttgttc agttatgac gacaaatgg 
      gtgtcggtg atggaacgg ccaaagaca tgcggggag 
      tatccgata aggttctac ggccgcgac tcggggttt 
      ttaaaattc cagattttt gccggcggc acaaggctg 
      atgtacccc tcagctcga agactggtg gcgttcact 
      ttccacccc acggaccct tttgccatt tcggtgcaa 
      cgaacaaac gccaattac gttgtaaat tttcacgta 
      agacaagac gcagcatat gctaataat aatttttac 
      gaagattgt aattttgta ctatga
    mRNA sequence
    • >TC013372 RA
      ctgttattc taaaaaatt gtcacgtga cgcgctttg 
      acatatcaa aatgagcta acctagaaa tcctcatat 
      caaaataat ggaaaaatc gccgatttg cagcaccga 
      atatgtatt caaacgtaa gaagatcgc ccctcaaaa 
      catcgtaat acgtctgaa aaatcgcga gacgttttc 
      gtaccgaaa accggggac tcacttcca caccgctcg 
      ccaattcta ccaatgtat tttccccaa ttttactgt 
      gataaacgt ggaaaagcc cccttgttt tttacgaaa 
      attcagtcc cgatggtaa atatttcat tgcgttttc 
      gtcggatca aacgtcgct ggaagtcta cacttacag 
      agggccggc agcggcggc cgacctatt caacaaaac 
      tgagaactg tgatgccga aaaacggtc agagattag 
      tagtaaaat ttttgatag gattttcaa gttaaaata 
      tgtggtaca tgtgaccca aggctctga ggaacttaa 
      tcgcgagtg tagcctttt cagtgatga cggcagata 
      cgtgataat cggttcagc ggcgttcat accggaaga 
      gatacggcc acactttta tgaaattta tacaaataa 
      cgaatcggt cacgcctaa cccgcgttc ccctttgga 
      aaattacac tcttcattt ggttgacct ccatcaagg 
      aaaactgtg tgattcgag gcaatttaa agtcgacaa 
      aattttcct gtcgcataa ccaaggcct ctatttata 
      caaggacat actcgctgt gctctcagt ccaacacca 
      agtgatcca cattttcca aatcctgga cggaatgtt 
      cgtaaatgt ccgaaaaat cggccgatt ttgcttcga 
      ggatgactc gtatttcta cgacttggc gtattcgaa 
      cgagagggc cttcaggga cgtgagcat aaactcgct 
      aaagcaccg attgctaac gtttttgta caaaagagc 
      agtaaccat aagtaaaag agacaacag tcccctaga 
      aatccgcaa atttttcca ccatttcag caactacaa 
      cgcactgaa actgtggaa aatgcagct tttggatga 
      gaaccactt gttgattaa gtacgcgag cgaggacgt 
      cgtcacgtt aaagtgcac cgacgcgaa cacccagcc 
      gtccttctt catagttta caacatgaa ggagagcaa 
      aattttggc aatttatga aaacacatc gactgattt 
      gttgcactt gtttgagaa tttctgtga ttcgtttag 
      gaacgcgtg cgttaactg cgagacgca gtttacttg 
      tagcccctc taataacat ttacgccag gattttgca 
      acaaaggtt caaacaaac catagtcag tgcaaaata 
      cggtggcga aacggaagc cactaaaag actcctagc 
      tcagttacc aataagtgc ccaatctta cagcagttc 
      gccttatct ggacttgtc gttgttcag ttatgacga 
      caaatgggt gtcggtgat ggaacggcc aaagacatg 
      cggggagta tccgataag gttctacgg ccgcgactc 
      ggggttttt aaaattcca gatttttgc cggcggcac 
      aaggctgat gtacccctc agctcgaag actggtggc 
      gttcacttt ccaccccac ggacccttt tgccatttc 
      ggtgcaacg aacaaacgc caattacgt tgtaaattt 
      tcacgtaag acaagacgc agcatatgc taataataa 
      tttttacga agattgtaa ttttgtact atgagattt 
      attgttgtg ataaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY