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Gene information
General information
TC013036 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14845585..14847825
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013036
    iBeetle screen iB_06310

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages partially position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013036 RA
      MLYILKEVE GPRFFYLFT GNEYDIGRK KAAIALTND 
      ASISRKHAI IKIGPNSAT LKDLESKYG TFHNKLKVT 
      PNTEIVLND KDIIKLGVY DSKFRIEKI DLVVTGSGL 
      TNEEKVELK GKITSIGGT FLNEWGPTC THLTAPVIV 
      LTIKMLHAL IDDKPIVNL KFWDEYILK IKNNEPLPE 
      PQNFKPPVT ETLINGTEL KPNPERKVL FKNKIFVFP 
      TEKLRLRMA YLIEKTGGS CISWEKQPL TFAQVTQSK 
      NTYLFIYSD DKNTPQSLK NIYEHLTKI GERTIPLQE 
      IPKAIVLCS CEKDCNPKF NRIFNVFVE KESDSESAI 
      FPLAYETPT DDVFAQTFE PKQELVIPE SLDADLHLL 
      TAPPTPNTS KQINNIITP SALKRGAKE PEKTPAKKV 
      KLDESANER KRKADENLG PSPKKNLLE NPFAIMRKK 
      IVNRPPEPD NPFVALRVK REVREETDN PFKNIMNNT 
      REKPAEKSS LIKSVQYSA DESYCPSDP SNVTWLSKH 
      DIKTESFEP ESDFKELFD LFKNTVVIE PLPPTAIRV 
      LPLSQYNSG NTTTGGKNF KKFKKVRPL RPQTDITRT 
      SIYQNMSVQ VIPEVVSDN EEEEVIRRP VPKKTNPRR 
      FQF
    CDS sequence
    • >TC013036 RA
      atgttatac attctgaag gaagttgaa ggtccaagg 
      tttttttat ttatttacc gggaacgaa tatgatatt 
      gggcgcaaa aaagcagct attgcgtta acaaatgat 
      gcatcaatt agccgaaaa cacgctatt attaaaatt 
      ggacccaat tcggcaact ttgaaggac ctagaatcg 
      aaatatggc acgttccat aataaatta aaagtaact 
      ccaaataca gaaattgtt ctaaatgat aaagacata 
      attaagctt ggagtttac gatagcaag tttagaatt 
      gaaaaaatt gatttggtg gtcacaggt tcgggttta 
      acgaatgaa gagaaggtg gaattaaaa ggcaaaatt 
      acttcaata gggggcaca tttctcaat gaatggggc 
      cccacttgt actcattta acagcacct gtaatagtg 
      ctaacaatc aaaatgttg cacgcgtta attgatgac 
      aaacctatc gttaattta aaattctgg gacgagtat 
      attctcaaa attaaaaat aacgaacct ttacctgaa 
      ccccaaaat tttaaacca ccagtaact gaaacttta 
      atcaacgga actgagcta aaaccaaac ccggaacgg 
      aaggtttta ttcaaaaat aagatattt gttttccca 
      acggagaag ctcagattg cggatggcg tatctcatt 
      gaaaaaaca gggggttcg tgtatttcg tgggagaaa 
      caaccccta acatttgca caagtgaca caatcgaaa 
      aacacttat cttttcata tacagcgac gataaaaac 
      acaccacaa tcgctcaaa aacatctac gaacatttg 
      actaaaata ggggaacgc actataccc ttgcaggaa 
      atccccaaa gctattgtg ctctgctct tgcgaaaaa 
      gactgtaat ccgaaattt aaccgcatc ttcaatgtt 
      ttcgtggaa aaagaatcg gactcagag tctgcgatt 
      tttccccta gcttacgaa acgccaacc gatgatgtg 
      tttgcgcag acttttgag cctaaacaa gagttggtg 
      attcctgag tcgctggat gcggacttg catttgcta 
      acggcgccc ccaactcca aatacttcc aaacaaatt 
      aataatatt attacacca agtgctttg aaacgaggg 
      gcaaaagaa ccggaaaaa acgcccgca aaaaaagta 
      aaattggac gaaagcgcc aatgagcgg aaacgcaaa 
      gctgatgag aatttgggc ccaagtccc aagaaaaac 
      ttactggag aatccgttc gcaataatg cggaaaaaa 
      atcgttaat aggcctcca gagccggat aatccgttc 
      gtcgctttg agggttaag agagaagtg cgggaggaa 
      acggataat ccgtttaaa aatatcatg aacaacacg 
      agggaaaaa ccagcggaa aaatcgagt ttaatcaag 
      tcggtacag tacagtgcg gatgagagc tactgtccc 
      tctgatcct tcaaatgtg acttggctg tccaagcac 
      gatattaaa accgagtct tttgagccg gaatccgac 
      tttaaggaa ctcttcgat ttgtttaaa aacactgta 
      gtgattgaa ccgttgcct ccaacggcc attagagtc 
      ctgcctttg agtcaatac aacagtggg aacaccacc 
      acaggcggg aagaacttt aagaaattt aaaaaagtg 
      cgccctttg aggccccaa accgacatt accagaacg 
      tcaatttat caaaatatg tcggtgcaa gtgatacca 
      gaagtagtg tctgataat gaggaggaa gaagtgatt 
      cgaaggccg gtgcccaaa aaaacgaat ccgagacgt 
      ttccaattt tag
    mRNA sequence
    • >TC013036 RA
      tgtcaagtg tttttaatg tttagatga ttttttagg 
      atttaaaat gtgtctgtg aaatgttgt gattgtcct 
      tgttgtgta attcccatt taataacat gttatacat 
      tctgaagga agttgaagg tccaaggtt tttttattt 
      atttaccgg gaacgaata tgatattgg gcgcaaaaa 
      agcagctat tgcgttaac aaatgatgc atcaattag 
      ccgaaaaca cgctattat taaaattgg acccaattc 
      ggcaacttt gaaggacct agaatcgaa atatggcac 
      gttccataa taaattaaa agtaactcc aaatacaga 
      aattgttct aaatgataa agacataat taagcttgg 
      agtttacga tagcaagtt tagaattga aaaaattga 
      tttggtggt cacaggttc gggtttaac gaatgaaga 
      gaaggtgga attaaaagg caaaattac ttcaatagg 
      gggcacatt tctcaatga atggggccc cacttgtac 
      tcatttaac agcacctgt aatagtgct aacaatcaa 
      aatgttgca cgcgttaat tgatgacaa acctatcgt 
      taatttaaa attctggga cgagtatat tctcaaaat 
      taaaaataa cgaaccttt acctgaacc ccaaaattt 
      taaaccacc agtaactga aactttaat caacggaac 
      tgagctaaa accaaaccc ggaacggaa ggttttatt 
      caaaaataa gatatttgt tttcccaac ggagaagct 
      cagattgcg gatggcgta tctcattga aaaaacagg 
      gggttcgtg tatttcgtg ggagaaaca acccctaac 
      atttgcaca agtgacaca atcgaaaaa cacttatct 
      tttcatata cagcgacga taaaaacac accacaatc 
      gctcaaaaa catctacga acatttgac taaaatagg 
      ggaacgcac tataccctt gcaggaaat ccccaaagc 
      tattgtgct ctgctcttg cgaaaaaga ctgtaatcc 
      gaaatttaa ccgcatctt caatgtttt cgtggaaaa 
      agaatcgga ctcagagtc tgcgatttt tcccctagc 
      ttacgaaac gccaaccga tgatgtgtt tgcgcagac 
      ttttgagcc taaacaaga gttggtgat tcctgagtc 
      gctggatgc ggacttgca tttgctaac ggcgccccc 
      aactccaaa tacttccaa acaaattaa taatattat 
      tacaccaag tgctttgaa acgaggggc aaaagaacc 
      ggaaaaaac gcccgcaaa aaaagtaaa attggacga 
      aagcgccaa tgagcggaa acgcaaagc tgatgagaa 
      tttgggccc aagtcccaa gaaaaactt actggagaa 
      tccgttcgc aataatgcg gaaaaaaat cgttaatag 
      gcctccaga gccggataa tccgttcgt cgctttgag 
      ggttaagag agaagtgcg ggaggaaac ggataatcc 
      gtttaaaaa tatcatgaa caacacgag ggaaaaacc 
      agcggaaaa atcgagttt aatcaagtc ggtacagta 
      cagtgcgga tgagagcta ctgtccctc tgatccttc 
      aaatgtgac ttggctgtc caagcacga tattaaaac 
      cgagtcttt tgagccgga atccgactt taaggaact 
      cttcgattt gtttaaaaa cactgtagt gattgaacc 
      gttgcctcc aacggccat tagagtcct gcctttgag 
      tcaatacaa cagtgggaa caccaccac aggcgggaa 
      gaactttaa gaaatttaa aaaagtgcg ccctttgag 
      gccccaaac cgacattac cagaacgtc aatttatca 
      aaatatgtc ggtgcaagt gataccaga agtagtgtc 
      tgataatga ggaggaaga agtgattcg aaggccggt 
      gcccaaaaa aacgaatcc gagacgttt ccaatttta 
      gtgtttttt tattgattt tattgtaaa cgctaataa 
      ttattaaaa ttttttact tcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY