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Gene information
General information
TC012765 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14489473..14491162
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012765
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06307

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 25.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012765 RA
      MRLKIALLL ILLTTTLIE SKRSRGRSR GTSSGGTGF 
      WNRKKSNES KPKTPASQP EIKKETSSV QQSAPTGNS 
      GRPIGWNVD NNSPMKKQN VNPAHTASA PYPQQNSGQ 
      GYGHGQPGT GHNYGQQTH GGYNYGHQT PGGYNYGHQ 
      TPGGYNYGQ QTPGGYNYG HQTPGGYNY GQQTPGGYN 
      YGQQTPGGY HTPGGYNAA GGYNYPGYH APGGYSAPG 
      YGGYPGHSP MGGYGGYGG YGGYGHSSG MFGNKYGGF 
      GGLGGYGGY YGMKKSGGF FGKHAFRNV LGGLFVWYL 
      VSGLVSKPY KVYNYYNQP KEIIQQEIA LPANVLTLC 
      NENATNICV PGTVAICTN DNRILCVTS MTSTTPCQD 
      NNGALCVNS TIPCLDTND PLCQNKTTS DNTTTVNLP 
      CFTNMTLDS VPPNITLPQ SDGNTFCVT TMAVPDPSM 
      PPVNVTEEP TNATLQVPG NGTQNAPMW YPPVPSNGT 
      QNGPMWYPP PPGNGTQNP PMWYPPPPP YWGYGPGYY 
      PPPPYWGQP PMNVTVTVP ANGTEATTE VTTVES
    CDS sequence
    • >TC012765 RA
      atgcgtctc aaaatcgcc cttttgctc attttactc 
      acaacgact ctaatcgag tcaaaacga tccagaggc 
      cggagtcgg ggcacgtcc tccggaggc acaggcttc 
      tggaaccgt aagaaatca aacgaatcc aagcccaaa 
      actccggca agtcaaccg gaaatcaag aaagaaacg 
      tccagcgtg caacaaagc gccccgacc ggaaactcc 
      gggaggccc atcggctgg aatgtggac aataactcg 
      cccatgaag aagcaaaat gtcaacccg gcccacacc 
      gccagcgcg ccgtacccg cagcagaac tccgggcaa 
      gggtacggg cacggccag cccggaact gggcacaat 
      tatggacaa caaacgcat gggggttac aattatgga 
      catcaaacg cctgggggt tacaattat ggacatcaa 
      acgcctggg ggttacaat tatggacaa caaacgcct 
      gggggttac aattatgga catcaaacg cctgggggt 
      tacaattat ggacaacaa acacctggg ggttacaac 
      tatggacaa caaacgcct ggaggttac cacactccg 
      gggggttac aacgcggca ggtggttac aattatccg 
      ggttaccac gctccgggt ggctacagc gctcctggt 
      tatggcgga tatccgggc catagtccg atggggggt 
      tacgggggt tatggaggt tatgggggt tatgggcac 
      tcaagcggc atgtttggt aacaaatat gggggcttt 
      gggggtctt ggtggttac ggcggttac tacggtatg 
      aaaaaatcc ggaggtttc ttcggaaag cacgctttc 
      cggaacgta ttaggcggc ctcttcgtt tggtattta 
      gtctcaggc cttgtaagt aagccttat aaggtatat 
      aattattac aaccaacct aaagagata atccagcaa 
      gaaatagca ctaccagca aacgtttta accttgtgt 
      aatgaaaac gctaccaat atttgtgta ccaggcact 
      gttgcgatt tgcaccaac gataacaga attctctgt 
      gttacaagc atgacttcg acaacccca tgccaggat 
      aacaatggg gctttgtgt gtcaatagt accatccct 
      tgcctagac accaacgac cctttatgc cagaataaa 
      accacaagt gataacaca acaacggta aacttaccg 
      tgtttcaca aacatgacc ctggactcg gtaccgcca 
      aatattacg ttaccacaa agtgatggt aacacgttc 
      tgtgttacc acaatggcg gtacctgac ccatccatg 
      cctccggtg aacgttacc gaagaacca acaaacgca 
      actctacaa gttccgggt aatgggacc caaaatgca 
      cccatgtgg tacccgcca gttccgagc aatgggacg 
      caaaatggg cccatgtgg tacccgcca ccgccaggc 
      aatggtacc caaaacccc ccgatgtgg tacccaccc 
      ccgcccccg tattggggc tatgggccc gggtactac 
      cctccaccc ccatactgg gggcagcca ccgatgaat 
      gtaacagta acagtacca gcaaatggt accgaagct 
      acaacggag gtgaccaca gtcgagtcg tga
    mRNA sequence
    • >TC012765 RA
      tcgtcacaa tgcgtctca aaatcgccc ttttgctca 
      ttttactca caacgactc taatcgagt caaaacgat 
      ccagaggcc ggagtcggg gcacgtcct ccggaggca 
      caggcttct ggaaccgta agaaatcaa acgaatcca 
      agcccaaaa ctccggcaa gtcaaccgg aaatcaaga 
      aagaaacgt ccagcgtgc aacaaagcg ccccgaccg 
      gaaactccg ggaggccca tcggctgga atgtggaca 
      ataactcgc ccatgaaga agcaaaatg tcaacccgg 
      cccacaccg ccagcgcgc cgtacccgc agcagaact 
      ccgggcaag ggtacgggc acggccagc ccggaactg 
      ggcacaatt atggacaac aaacgcatg ggggttaca 
      attatggac atcaaacgc ctgggggtt acaattatg 
      gacatcaaa cgcctgggg gttacaatt atggacaac 
      aaacgcctg ggggttaca attatggac atcaaacgc 
      ctgggggtt acaattatg gacaacaaa cacctgggg 
      gttacaact atggacaac aaacgcctg gaggttacc 
      acactccgg ggggttaca acgcggcag gtggttaca 
      attatccgg gttaccacg ctccgggtg gctacagcg 
      ctcctggtt atggcggat atccgggcc atagtccga 
      tggggggtt acgggggtt atggaggtt atgggggtt 
      atgggcact caagcggca tgtttggta acaaatatg 
      ggggctttg ggggtcttg gtggttacg gcggttact 
      acggtatga aaaaatccg gaggtttct tcggaaagc 
      acgctttcc ggaacgtat taggcggcc tcttcgttt 
      ggtatttag tctcaggcc ttgtaagta agccttata 
      aggtatata attattaca accaaccta aagagataa 
      tccagcaag aaatagcac taccagcaa acgttttaa 
      ccttgtgta atgaaaacg ctaccaata tttgtgtac 
      caggcactg ttgcgattt gcaccaacg ataacagaa 
      ttctctgtg ttacaagca tgacttcga caaccccat 
      gccaggata acaatgggg ctttgtgtg tcaatagta 
      ccatccctt gcctagaca ccaacgacc ctttatgcc 
      agaataaaa ccacaagtg ataacacaa caacggtaa 
      acttaccgt gtttcacaa acatgaccc tggactcgg 
      taccgccaa atattacgt taccacaaa gtgatggta 
      acacgttct gtgttacca caatggcgg tacctgacc 
      catccatgc ctccggtga acgttaccg aagaaccaa 
      caaacgcaa ctctacaag ttccgggta atgggaccc 
      aaaatgcac ccatgtggt acccgccag ttccgagca 
      atgggacgc aaaatgggc ccatgtggt acccgccac 
      cgccaggca atggtaccc aaaaccccc cgatgtggt 
      acccacccc cgcccccgt attggggct atgggcccg 
      ggtactacc ctccacccc catactggg ggcagccac 
      cgatgaatg taacagtaa cagtaccag caaatggta 
      ccgaagcta caacggagg tgaccacag tcgagtcgt 
      gataaccta gtcttatta tttattctc aatgtttgt 
      agacagtac aaagaaata aagaatgaa aactgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY