Status
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Gene information
General information
TC034425
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012659
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12617484..12654164
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034425
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034425 RA
      MPRESSKCR EWEKERRNR LNEAFATLC KLLPCYDPA 
      TNVSKIDIL RNAASYIEE LQTKVKTLV SQDNDEPAK 
      KIKGEELKK LQERIRKLL SKNEQLCAL LREAKITIP 
      PGCSLRKFK PPFNWCNKI MPEQAKILQ KKELDKENN 
      ASAKKLQGR KFTNKSRNK NSTPKHRKA ILNRSDSKK 
      LRPSKKKKI KPIPTSKCV IVLSQPNAT QPCYIITNA 
      PSNNILGGS STVITNSTL VANPLVSSK AAKTVASMP 
      PLGPGTLIL ANGTIMPVL PQQTVLPLA PQIITRPPP 
      IILLPRNPV TTSTPTTTA KTITTTTSV KTLTRIKPK 
      YCLTRTTQV NKVPIPALT SRYSNPEVV LKKNEPKAK 
      AKPVEKKVT KEGEKKSEG EKRKEATPD DNCTEAKKA 
      KPSEDKENE KEVSEKVAE TVEVQAKCV EEKEKEAEE 
      KPENEPKTT SEPEQKKPD ETLELNINS ELSNDIFAS 
      LQVPAGCQN PESTSPTAA FLLAFPLVS SLTGVKVTE 
      VTEEDNTES QRGTPTLLQ IGTMDTTKP TQSESLTPS 
      LLNLDNLSF FSSKDIGGF YSSFEATAT TVAVSTVTT 
      VTPSVSKEE KQKQVKNQP VPYYDNHNL APIPTFCST 
      NSTNSVSQM SYQAKPAEA KCSNYNTQK MFCNSFDTP 
      KVTTTCSYA NQIKSYPEP LYTNSSTYT YSNTSDSGF 
      NQNYYKELP KTDNRSYYS LNYENYTDY RKNDSNTYF 
      PTSNYYTNG QKFATNNNT NNAQKPKNV PQTSRAPIN 
      WMTTPDNRD YVLPQFTTK ESDFGHNSI YTIQSCNTF 
      TNPQTTYFN TNPMYPSNT NDVGNNPQE TRKSLDLPI 
      YQEENQFSW SPSKIPQFL DPPHSFVSS TLPTLVGDL 
      ALGNPLTFG EQKTDKCQK KRVKYEGNQ NFLSVSQLV 
      DHNKEAPAR TTARRNSGN RGTSGKKVP QKTAQRKEN 
      KVETGKGMK QLGYSSDLF CETKNRNVA KNSYSAEAL 
      IGPQMQADG GHKNRNYAG NKAISVSSF LHDNIIPYF 
      PTVDLPQDN TYMQQNQNY QTSSFSHSS YTSNSFIPS 
      TTTYNFMHD VHDYSENLA LFPPVTKDK SCKIVNRNV 
      REQQCAVAP KKSKRKHSN ETNLPGFVD FPFLSMPGS 
      INSPILPDD FHTHSGFLQ PPTPQLYPC KNPFTKSND 
      LGSGALLPL PPVPPKALS KGLFPYKNN EHCTVKRGN 
      ENVASSRRA TIVWSTVMF MVPSSFLVH LHRYLGHSV 
      FHFVRLATH QAKLAKMAL FCALSNEVP EHPVVSPTS 
      GAIFEKRII EKYIQEHGK DPISGDELT VDKLIEIKT 
      PPIVKPKPP SATSIPATL KLLQDEWDA VMLYSFTQR 
      QQLQTARQE LSHALYQHD AACRVIARL NKEVTAARE 
      ALATLKPQA GIITVPQPA VAAEAGGIA NQPTEQCGL 
      TDEVIEKLQ DKATVLTQE RKKRSKTVP EEVSTPEQL 
      RGFKTLASH PGLHSASVP GILALDVHN SDTSKILTG 
      GNDRNATVF NKDTEQVVA ILKGHTKKV TKVVYHPDE 
      DLVITASPD STIRVWNVP TSQTTLLLR VHEGPITGL 
      SLHPTGDYV LSASEDQHW AFSDIRTGE LLTKVADQS 
      NIPLTTAQL HPDGLIFGT GTANSQVKI WDLKEQLNV 
      ATCTGHAGA ITAISFSEN GYYMATADD ACVKLWDLR 
      KVRNFKTLQ LDEDYQIKD LCFDQSGTY LAVAGTDVR 
      VYFCKQWQE LKVFNDHTA AATGVRFGK DARFIASTS 
      MDRTLKLYG LD
    CDS sequence
    • >TC034425 RA
      atgcccaga gagtcaagt aagtgtcgc gaatgggaa 
      aaagaacga aggaatcgt ttaaatgag gcttttgcc 
      acgttatgt aaattattg ccatgctat gacccggct 
      acgaacgtt agtaaaatc gatattttg cgtaatgct 
      gccagttac attgaggag ctccaaacc aaggttaaa 
      accctagtc tcgcaagac aatgacgaa ccggctaaa 
      aaaatcaag ggcgaagag cttaaaaaa ttgcaagaa 
      cgtatcagg aaacttctc agtaaaaat gagcagttg 
      tgcgccctt ttacgtgaa gcgaagatc acaatcccg 
      ccagggtgc tccctcagg aagttcaaa cctccgttt 
      aattggtgt aataaaatt atgcccgag caggccaag 
      attttacaa aagaaggag ctcgacaag gaaaataat 
      gcttcggcc aagaaactc caaggccgc aaattcaca 
      aataagagc cggaacaaa aacagcacg cccaagcac 
      agaaaagca attttgaac aggtccgac tctaaaaaa 
      ttgcgcccc tcgaagaaa aagaaaatt aaaccaata 
      ccaacttca aaatgtgtg attgtgttg tcgcaaccg 
      aatgccaca caaccgtgt tacataatc acaaacgcg 
      ccttcaaac aatattttg ggtggttct tccactgtc 
      attacgaac tcaactctt gttgctaat cctttggtg 
      tcgtcgaaa gcggccaag accgttgct tccatgccg 
      cccctcggc cccggcact ttgattttg gccaacggt 
      accataatg ccggtacta ccacagcaa accgtcctt 
      ccgctcgct cctcaaatc attaccaga ccgccaccc 
      atcatccta ttaccgcga aaccccgtc actacttcc 
      acccccaca actaccgcc aaaaccatc accaccact 
      acaagtgtc aagactctg acccgcatc aagccaaag 
      tattgcctc acacgaaca actcaggtc aataaagtc 
      ccgattccc gcactgact tcgcgttat tccaatccc 
      gaggtcgta cttaagaaa aacgagccc aaagctaag 
      gcaaagccc gtggaaaag aaggttacc aaagagggt 
      gaaaaaaag agtgaaggg gaaaaacga aaagaggcg 
      acgcctgac gataattgc accgaagcc aagaaggcg 
      aaacccagc gaggataaa gaaaacgaa aaggaagtt 
      tccgagaaa gtggccgaa actgtggag gttcaagcc 
      aagtgcgtg gaagaaaag gagaaagaa gcggaagag 
      aaacccgaa aatgagcct aaaaccacc agcgaaccc 
      gagcagaaa aaaccggac gagacgctt gaattaaac 
      ataaattcg gaactatcg aacgatatt ttcgcctct 
      ttgcaagta ccggccggg tgccaaaac cccgaatca 
      acgtccccc acggccgcc ttcctcttg gctttcccc 
      ctggtgtcg tcactcacc ggggttaag gtcaccgaa 
      gtgaccgaa gaagacaat acggagagt cagagaggc 
      acccccacg ttgctccag atcggtacc atggacacc 
      accaagccc acacaatcc gaaagtttg acgcccagt 
      ttactgaat ctcgataac ttatcgttt ttttcgtcg 
      aaagatatc ggcggtttt tacagctcg tttgaggca 
      actgccaca acagtggcc gtcagtacc gtcacaact 
      gttaccccg agcgttagc aaagaagag aagcaaaag 
      caagtgaag aaccagcca gtaccgtat tatgacaac 
      cacaatctt gcccccata ccgactttt tgtagtact 
      aatagtacg aattcagtc agtcaaatg agctatcaa 
      gcgaaacct gcggaagca aagtgttcg aattacaac 
      acccagaag atgttctgc aatagcttc gacaccccc 
      aaagttaca accacttgc tcgtacgcc aaccaaatc 
      aagagttac ccagaaccg ctgtatacc aacagttcc 
      acctacact tacagtaac acctctgat agcggtttc 
      aatcagaac tactacaag gaactgcca aagactgac 
      aataggagt tactattcg ttgaattat gagaattat 
      acggattac cgcaaaaat gattctaat acgtacttc 
      cccacctcg aactattac accaatggg cagaaattc 
      gccacgaat aataacacc aacaacgcc caaaaaccc 
      aaaaatgtg ccccagact agcagagct ccgatcaat 
      tggatgaca acacccgac aatcgcgac tacgttttg 
      ccccaattt acgaccaaa gagagcgat tttggccac 
      aactcaatc tacacgatc caatcgtgc aatactttc 
      accaaccca caaaccacg tacttcaac acgaacccc 
      atgtaccca tcaaacacc aacgacgtg gggaacaac 
      ccacaggaa acgcgcaaa agcctcgat ttgcccatc 
      taccaggag gagaaccaa ttctcatgg tcaccctcc 
      aaaataccg caattcctg gacccaccc catagtttc 
      gtctcgtcg actctccca acgctagtc ggtgaccta 
      gccttgggg aacccccta accttcggc gaacaaaag 
      accgacaag tgccaaaag aaacgagtg aagtacgag 
      ggcaaccag aacttttta tcagttagt cagttggtt 
      gaccacaac aaggaagca ccagcaagg accacagcc 
      cggcggaac agcggcaat cggggcact tcgggcaag 
      aaagtcccc cagaaaacg gcccaaaga aaggagaat 
      aaggtggag acgggcaaa gggatgaaa caattgggg 
      tattcaagt gacttgttt tgcgagacg aaaaatcgc 
      aacgttgcg aaaaacagc tactcggcc gaggcgcta 
      atcgggccc cagatgcaa gccgacggg ggccacaag 
      aaccgcaac tatgccggc aataaggcc atttcggtg 
      tcctcgttc ctccacgat aacataatc ccgtatttc 
      ccgaccgtt gacttgccg caggacaat acgtacatg 
      cagcagaac cagaactac cagacgagt tcgttcagt 
      cacagctcc tacacctcc aactcgttc attccctcg 
      accacgacg tacaacttc atgcacgac gtgcacgac 
      tacagcgag aacctggcg ctgttcccg cccgtgacc 
      aaggacaag agctgcaag attgttaac aggaatgtg 
      cgggagcag cagtgtgcc gtggcgccg aaaaagtcg 
      aaacggaag cactcgaac gagacgaat ctgccgggt 
      tttgtcgat tttccgttt ctgtcgatg ccgggctcg 
      attaattcg ccgattttg cccgatgat tttcacacg 
      catagtggg tttttgcag ccgccgacg ccgcagctc 
      tatccgtgt aagaatccg tttacgaag agtaatgat 
      ttggggtcg ggggcgttg ctgccgttg cctccggtt 
      ccgccgaag gccctatcg aagggctta ttcccctac 
      aaaaataat gaacattgc acggtaaaa aggggaaac 
      gagaatgtt gctagctcc aggcgcgca acgattgtc 
      tggtcgact gtaatgttc atggtgcca tcatcattt 
      ttagtgcat ctgcacaga tatctcggc cacagtgtt 
      tttcacttt gtgcggttg gcgacacat caagctaag 
      ttagcaaaa atggcgttg ttttgtgcc ctttcaaac 
      gaggtgccg gaacaccca gtcgtgtcc ccaacgtcg 
      ggtgcgatt ttcgaaaaa cgcatcatc gaaaagtac 
      atccaggag cacggcaag gaccccatc agcggtgac 
      gagctcacc gtggacaag ctcattgaa attaagacc 
      ccgccgata gtcaagccg aagccgccg agcgccacc 
      agcatcccg gccacgctg aagctgctg caggacgag 
      tgggacgcc gtgatgctg tacagcttc acgcagagg 
      cagcagctg cagacggcg cgccaggag ctgagccac 
      gcgctctac cagcacgac gccgcgtgt cgcgtgatc 
      gcgcgcctc aacaaggag gtgaccgcc gccagggag 
      gcgctggcc acgctgaag ccccaagcg ggcatcatc 
      acggtgccg cagcccgcg gtggcggcc gaggccggc 
      ggcatcgcc aaccagccg acagagcaa tgcggcctg 
      actgatgaa gtgattgag aagctgcag gacaaggcc 
      acggtgttg acgcaggag aggaagaag aggagcaag 
      acggtgcct gaagaggtg tccacgccg gagcagttg 
      aggggattc aagactttg gcctcacat ccggggctg 
      cattcggcg agcgtcccc ggtattttg gcccttgac 
      gtgcacaat tccgacaca agcaaaatt ctgaccggt 
      ggtaatgat cgcaacgcc accgtattc aacaaagac 
      acggaacaa gttgtggcg attctcaaa ggccacact 
      aaaaaagtc acaaaagtg gtctaccac cctgacgag 
      gaccttgtt atcaccgca tcgcccgac tccacaatc 
      cgcgtttgg aacgtgcca acgtcccaa accactcta 
      ctgctgcgc gtccacgaa ggtccaatc accggactg 
      tccctgcat ccgactggg gactacgtc ctgtcggct 
      tcagaggac cagcattgg gcgttttcg gacatcaga 
      acgggcgaa ctgttgacg aaagtcgcc gaccagagc 
      aatattccc ctgacgacg gcccagctg catccagac 
      gggctgatt ttcggcacg gggacggcg aattcccaa 
      gtcaagatt tgggacctg aaggagcag ctcaatgtt 
      gcgacttgc accggacat gcgggggcg atcacagcc 
      atttcgttc tcggagaat gggtattac atggcgacg 
      gctgacgat gcctgcgtc aagttgtgg gatttgagg 
      aaagtgagg aactttaaa acgctccag ttggatgag 
      gattaccaa atcaaggac ttgtgcttt gatcagagc 
      gggacctat ttggcggtg gcgggaact gatgtcagg 
      gtgtatttc tgtaagcaa tggcaggaa ctgaaggtg 
      ttcaatgac cacacggct gcagcgacg ggtgttagg 
      ttcggtaaa gacgccagg tttatagct tccacttcc 
      atggacagg acattaaaa ttgtacggt ttagattag
    mRNA sequence
    • >TC034425 RA
      ttatgttgt aaataaatt tgttatctg ttgaggacc 
      taaaggctt gaaatatcg ttctaattt attgaaaat 
      gcggtttcg cttaattta ttgttataa ttatgccca 
      gagagtcaa gtaagtgtc gcgaatggg aaaaagaac 
      gaaggaatc gtttaaatg aggcttttg ccacgttat 
      gtaaattat tgccatgct atgacccgg ctacgaacg 
      ttagtaaaa tcgatattt tgcgtaatg ctgccagtt 
      acattgagg agctccaaa ccaaggtta aaaccctag 
      tctcgcaag acaatgacg aaccggcta aaaaaatca 
      agggcgaag agcttaaaa aattgcaag aacgtatca 
      ggaaacttc tcagtaaaa atgagcagt tgtgcgccc 
      ttttacgtg aagcgaaga tcacaatcc cgccagggt 
      gctccctca ggaagttca aacctccgt ttaattggt 
      gtaataaaa ttatgcccg agcaggcca agattttac 
      aaaagaagg agctcgaca aggaaaata atgcttcgg 
      ccaagaaac tccaaggcc gcaaattca caaataaga 
      gccggaaca aaaacagca cgcccaagc acagaaaag 
      caattttga acaggtccg actctaaaa aattgcgcc 
      cctcgaaga aaaagaaaa ttaaaccaa taccaactt 
      caaaatgtg tgattgtgt tgtcgcaac cgaatgcca 
      cacaaccgt gttacataa tcacaaacg cgccttcaa 
      acaatattt tgggtggtt cttccactg tcattacga 
      actcaactc ttgttgcta atcctttgg tgtcgtcga 
      aagcggcca agaccgttg cttccatgc cgcccctcg 
      gccccggca ctttgattt tggccaacg gtaccataa 
      tgccggtac taccacagc aaaccgtcc ttccgctcg 
      ctcctcaaa tcattacca gaccgccac ccatcatcc 
      tattaccgc gaaaccccg tcactactt ccaccccca 
      caactaccg ccaaaacca tcaccacca ctacaagtg 
      tcaagactc tgacccgca tcaagccaa agtattgcc 
      tcacacgaa caactcagg tcaataaag tcccgattc 
      ccgcactga cttcgcgtt attccaatc ccgaggtcg 
      tacttaaga aaaacgagc ccaaagcta aggcaaagc 
      ccgtggaaa agaaggtta ccaaagagg gtgaaaaaa 
      agagtgaag gggaaaaac gaaaagagg cgacgcctg 
      acgataatt gcaccgaag ccaagaagg cgaaaccca 
      gcgaggata aagaaaacg aaaaggaag tttccgaga 
      aagtggccg aaactgtgg aggttcaag ccaagtgcg 
      tggaagaaa aggagaaag aagcggaag agaaacccg 
      aaaatgagc ctaaaacca ccagcgaac ccgagcaga 
      aaaaaccgg acgagacgc ttgaattaa acataaatt 
      cggaactat cgaacgata ttttcgcct ctttgcaag 
      taccggccg ggtgccaaa accccgaat caacgtccc 
      ccacggccg ccttcctct tggctttcc ccctggtgt 
      cgtcactca ccggggtta aggtcaccg aagtgaccg 
      aagaagaca atacggaga gtcagagag gcaccccca 
      cgttgctcc agatcggta ccatggaca ccaccaagc 
      ccacacaat ccgaaagtt tgacgccca gtttactga 
      atctcgata acttatcgt ttttttcgt cgaaagata 
      tcggcggtt tttacagct cgtttgagg caactgcca 
      caacagtgg ccgtcagta ccgtcacaa ctgttaccc 
      cgagcgtta gcaaagaag agaagcaaa agcaagtga 
      agaaccagc cagtaccgt attatgaca accacaatc 
      ttgccccca taccgactt tttgtagta ctaatagta 
      cgaattcag tcagtcaaa tgagctatc aagcgaaac 
      ctgcggaag caaagtgtt cgaattaca acacccaga 
      agatgttct gcaatagct tcgacaccc ccaaagtta 
      caaccactt gctcgtacg ccaaccaaa tcaagagtt 
      acccagaac cgctgtata ccaacagtt ccacctaca 
      cttacagta acacctctg atagcggtt tcaatcaga 
      actactaca aggaactgc caaagactg acaatagga 
      gttactatt cgttgaatt atgagaatt atacggatt 
      accgcaaaa atgattcta atacgtact tccccacct 
      cgaactatt acaccaatg ggcagaaat tcgccacga 
      ataataaca ccaacaacg cccaaaaac ccaaaaatg 
      tgccccaga ctagcagag ctccgatca attggatga 
      caacacccg acaatcgcg actacgttt tgccccaat 
      ttacgacca aagagagcg attttggcc acaactcaa 
      tctacacga tccaatcgt gcaatactt tcaccaacc 
      cacaaacca cgtacttca acacgaacc ccatgtacc 
      catcaaaca ccaacgacg tggggaaca acccacagg 
      aaacgcgca aaagcctcg atttgccca tctaccagg 
      aggagaacc aattctcat ggtcaccct ccaaaatac 
      cgcaattcc tggacccac cccatagtt tcgtctcgt 
      cgactctcc caacgctag tcggtgacc tagccttgg 
      ggaaccccc taaccttcg gcgaacaaa agaccgaca 
      agtgccaaa agaaacgag tgaagtacg agggcaacc 
      agaactttt tatcagtta gtcagttgg ttgaccaca 
      acaaggaag caccagcaa ggaccacag cccggcgga 
      acagcggca atcggggca cttcgggca agaaagtcc 
      cccagaaaa cggcccaaa gaaaggaga ataaggtgg 
      agacgggca aagggatga aacaattgg ggtattcaa 
      gtgacttgt tttgcgaga cgaaaaatc gcaacgttg 
      cgaaaaaca gctactcgg ccgaggcgc taatcgggc 
      cccagatgc aagccgacg ggggccaca agaaccgca 
      actatgccg gcaataagg ccatttcgg tgtcctcgt 
      tcctccacg ataacataa tcccgtatt tcccgaccg 
      ttgacttgc cgcaggaca atacgtaca tgcagcaga 
      accagaact accagacga gttcgttca gtcacagct 
      cctacacct ccaactcgt tcattccct cgaccacga 
      cgtacaact tcatgcacg acgtgcacg actacagcg 
      agaacctgg cgctgttcc cgcccgtga ccaaggaca 
      agagctgca agattgtta acaggaatg tgcgggagc 
      agcagtgtg ccgtggcgc cgaaaaagt cgaaacgga 
      agcactcga acgagacga atctgccgg gttttgtcg 
      attttccgt ttctgtcga tgccgggct cgattaatt 
      cgccgattt tgcccgatg attttcaca cgcatagtg 
      ggtttttgc agccgccga cgccgcagc tctatccgt 
      gtaagaatc cgtttacga agagtaatg atttggggt 
      cgggggcgt tgctgccgt tgcctccgg ttccgccga 
      aggccctat cgaagggct tattcccct acaaaaata 
      atgaacatt gcacggtaa aaaggggaa acgagaatg 
      ttgctagct ccaggcgcg caacgattg tctggtcga 
      ctgtaatgt tcatggtgc catcatcat ttttagtgc 
      atctgcaca gatatctcg gccacagtg tttttcact 
      ttgtgcggt tggcgacac atcaagcta agttagcaa 
      aaatggcgt tgttttgtg ccctttcaa acgaggtgc 
      cggaacacc cagtcgtgt ccccaacgt cgggtgcga 
      ttttcgaaa aacgcatca tcgaaaagt acatccagg 
      agcacggca aggacccca tcagcggtg acgagctca 
      ccgtggaca agctcattg aaattaaga ccccgccga 
      tagtcaagc cgaagccgc cgagcgcca ccagcatcc 
      cggccacgc tgaagctgc tgcaggacg agtgggacg 
      ccgtgatgc tgtacagct tcacgcaga ggcagcagc 
      tgcagacgg cgcgccagg agctgagcc acgcgctct 
      accagcacg acgccgcgt gtcgcgtga tcgcgcgcc 
      tcaacaagg aggtgaccg ccgccaggg aggcgctgg 
      ccacgctga agccccaag cgggcatca tcacggtgc 
      cgcagcccg cggtggcgg ccgaggccg gcggcatcg 
      ccaaccagc cgacagagc aatgcggcc tgactgatg 
      aagtgattg agaagctgc aggacaagg ccacggtgt 
      tgacgcagg agaggaaga agaggagca agacggtgc 
      ctgaagagg tgtccacgc cggagcagt tgaggggat 
      tcaagactt tggcctcac atccggggc tgcattcgg 
      cgagcgtcc ccggtattt tggcccttg acgtgcaca 
      attccgaca caagcaaaa ttctgaccg gtggtaatg 
      atcgcaacg ccaccgtat tcaacaaag acacggaac 
      aagttgtgg cgattctca aaggccaca ctaaaaaag 
      tcacaaaag tggtctacc accctgacg aggaccttg 
      ttatcaccg catcgcccg actccacaa tccgcgttt 
      ggaacgtgc caacgtccc aaaccactc tactgctgc 
      gcgtccacg aaggtccaa tcaccggac tgtccctgc 
      atccgactg gggactacg tcctgtcgg cttcagagg 
      accagcatt gggcgtttt cggacatca gaacgggcg 
      aactgttga cgaaagtcg ccgaccaga gcaatattc 
      ccctgacga cggcccagc tgcatccag acgggctga 
      ttttcggca cggggacgg cgaattccc aagtcaaga 
      tttgggacc tgaaggagc agctcaatg ttgcgactt 
      gcaccggac atgcggggg cgatcacag ccatttcgt 
      tctcggaga atgggtatt acatggcga cggctgacg 
      atgcctgcg tcaagttgt gggatttga ggaaagtga 
      ggaacttta aaacgctcc agttggatg aggattacc 
      aaatcaagg acttgtgct ttgatcaga gcgggacct 
      atttggcgg tggcgggaa ctgatgtca gggtgtatt 
      tctgtaagc aatggcagg aactgaagg tgttcaatg 
      accacacgg ctgcagcga cgggtgtta ggttcggta 
      aagacgcca ggtttatag cttccactt ccatggaca 
      ggacattaa aattgtacg gtttagatt agggataaa 
      ttgactgcg agttgtaca caatagttt gtcgtcttg 
      catttcgca taattaggt cttaaagta cgattactt 
      tatgttaat gtttatttt aattttaca aataaagtg 
      attaaatct ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY