Status
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Gene information
General information
TC012524 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10994765..11006956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012524
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06303

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (22 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles distal orientation irregular - Number of animals showing the phenotype: 10

    dorsal thoracic muscles thinner - Number of animals showing the phenotype: 10
    longitudinal dorsal thoracic muscles median thinner - Number of animals showing the phenotype: 6

    longitudinal dorsal thoracic muscles sometimes irregular - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012524 RA
      MSFRRGEYP YGGGDKNGG VQPMQHGEE SSERAKTQQ 
      PVKLQPSWE ENNLPEDEL NFKNQTMDD ASLALNQPP 
      DKYNLVYLT FLIHGIGVL TPWNMFITA DKYFTEHKL 
      SEEYTGEIL PYVTNFMQY LTFASQVPN VFFNWLNIF 
      IQIGGNLTT RIVWSISIE VVVFIVTIV LAMIDTSTW 
      PVPFFWITM VCVVILNMA NGIYQNTVF GMAAKLPGK 
      YTGAVILGS NISGTFTAV VSLLSTIMA SNKKMAAIY 
      YFITALFVL LVCFDTYFA LPLNRFYRH HELREKKNA 
      ELRKQMNQG RTQRIPYLH ILKKSLPQL YNVFFIFFV 
      TLSIFPAIQ TNVKRGDEN FFIGEDYYT GITCFLTFN 
      VCAMVGSYL TSLLRWPGP KYLWIFVTL RVLYIPFFF 
      FCNYQINGI ERHIPVYVT SDWVYWIVA ITMGLTSGY 
      FSSLAMMYT PGCVEERYS STAGMFAAA SLITGIFTG 
      ILSTFLWPW IIKHVKY
    CDS sequence
    • >TC012524 RA
      atgagtttc agaagagga gagtatcct tacggagga 
      ggcgataaa aatggaggt gtacagccc atgcaacac 
      ggggaggag agtagcgaa cgagccaaa acccaacaa 
      cccgtcaaa ctgcaacca tcctgggag gagaacaat 
      ttacccgaa gacgaattg aattttaag aaccaaacc 
      atggacgac gccagtctg gccctaaac cagcctccg 
      gacaaatat aatttagtg tatttgact tttttgatc 
      cacggaatt ggagtttta acgccttgg aacatgttt 
      atcactgcc gataagtat tttacagaa cacaaattg 
      tcagaggag tacactggc gagatttta ccgtacgtg 
      acgaatttt atgcaatat ctcactttt gcttcccaa 
      gttcctaat gtgtttttc aattggctt aatattttt 
      atacaaatc ggtggtaat ttaaccacg cgtatagtt 
      tggagcatt agtatcgaa gtagtcgtt tttatagta 
      actattgta cttgcaatg atagatacg agcacgtgg 
      ccagttccg tttttttgg ataacgatg gtgtgcgta 
      gttattttg aatatggcc aatggcatt tatcagaac 
      accgtgttc ggaatggcc gccaaactg ccagggaaa 
      tacacagga gctgtgatt ttaggctct aatattagc 
      gggactttc acagctgtt gtctcgctg ttatcgacc 
      ataatggcc tcgaataaa aaaatggca gccatttat 
      tacttcatc acggcgctt tttgtgctg ctcgtttgc 
      tttgacacc tactttgcc ttacccctc aatcgattc 
      taccgccac cacgagctc cgcgagaag aaaaacgcg 
      gaactgcga aaacaaatg aaccaggga cgcacccaa 
      cgcatcccc tacttgcac atcctcaaa aaatccctc 
      ccccaactt tacaacgtc tttttcata ttcttcgta 
      accctatca attttccca gctatacag acaaacgtt 
      aaacgtggt gacgagaat ttttttatt ggcgaggac 
      tattacaca ggaattacg tgtttcctc acttttaat 
      gtttgtgcg atggtgggc agttatctg actagctta 
      ctgcgttgg cccggtccg aaatattta tggattttt 
      gtcactctc cgagtgttg tatatcccg tttttcttt 
      ttttgtaat taccagatc aacgggatt gagcggcat 
      attccggtg tacgtcacg agtgattgg gtttattgg 
      atagtcgcc atcacaatg gggctgact agtgggtac 
      tttagctcc ctggccatg atgtacacc ccagggtgc 
      gtggaagag agatattcc tcgacggcg ggcatgttc 
      gccgccgct tctttgata actggtatc tttaccggg 
      attttgtcg acattttta tggccttgg ataatcaag 
      catgtcaaa tactag
    mRNA sequence
    • >TC012524 RA
      cgggagcga gaaggaacc tcaatggaa aaaggacgg 
      caacagagg tggggtaaa agaaaatgg tactggtaa 
      tggccgtct aacggttgt gtagtatgt ccgttgcag 
      ttgcctgta tatattcat tttatcaat gcgaaaagc 
      acgtgttta aaatgcgtg cctgccaat tcgaatttt 
      gatagtgcg actagtgac ggaaagact gtttggaag 
      aatgattta ctgattaga gctgggaag attgttatc 
      aggagggac aatgagttt cagaagagg agagtatcc 
      ttacggagg aggcgataa aaatggagg tgtacagcc 
      catgcaaca cggggagga gagtagcga acgagccaa 
      aacccaaca acccgtcaa actgcaacc atcctggga 
      ggagaacaa tttacccga agacgaatt gaattttaa 
      gaaccaaac catggacga cgccagtct ggccctaaa 
      ccagcctcc ggacaaata taatttagt gtatttgac 
      ttttttgat ccacggaat tggagtttt aacgccttg 
      gaacatgtt tatcactgc cgataagta ttttacaga 
      acacaaatt gtcagagga gtacactgg cgagatttt 
      accgtacgt gacgaattt tatgcaata tctcacttt 
      tgcttccca agttcctaa tgtgttttt caattggct 
      taatatttt tatacaaat cggtggtaa tttaaccac 
      gcgtatagt ttggagcat tagtatcga agtagtcgt 
      ttttatagt aactattgt acttgcaat gatagatac 
      gagcacgtg gccagttcc gtttttttg gataacgat 
      ggtgtgcgt agttatttt gaatatggc caatggcat 
      ttatcagaa caccgtgtt cggaatggc cgccaaact 
      gccagggaa atacacagg agctgtgat tttaggctc 
      taatattag cgggacttt cacagctgt tgtctcgct 
      gttatcgac cataatggc ctcgaataa aaaaatggc 
      agccattta ttacttcat cacggcgct ttttgtgct 
      gctcgtttg ctttgacac ctactttgc cttacccct 
      caatcgatt ctaccgcca ccacgagct ccgcgagaa 
      gaaaaacgc ggaactgcg aaaacaaat gaaccaggg 
      acgcaccca acgcatccc ctacttgca catcctcaa 
      aaaatccct cccccaact ttacaacgt ctttttcat 
      attcttcgt aaccctatc aattttccc agctataca 
      gacaaacgt taaacgtgg tgacgagaa tttttttat 
      tggcgagga ctattacac aggaattac gtgtttcct 
      cacttttaa tgtttgtgc gatggtggg cagttatct 
      gactagctt actgcgttg gcccggtcc gaaatattt 
      atggatttt tgtcactct ccgagtgtt gtatatccc 
      gtttttctt tttttgtaa ttaccagat caacgggat 
      tgagcggca tattccggt gtacgtcac gagtgattg 
      ggtttattg gatagtcgc catcacaat ggggctgac 
      tagtgggta ctttagctc cctggccat gatgtacac 
      cccagggtg cgtggaaga gagatattc ctcgacggc 
      gggcatgtt cgccgccgc ttctttgat aactggtat 
      ctttaccgg gattttgtc gacattttt atggccttg 
      gataatcaa gcatgtcaa atactagca aaagtgttt 
      ggtatatga tacggaaac ggaattatc tatacaatt 
      ttagatagt agtttctgt catatgcca tagagaaac 
      aagtatgat ttcaatggg tcccgaatc atagttctt 
      ccaactaac gaagaatat tgttttgta gcagttttg 
      taaatagtt ttagtccca ttggctttt caaaaatgt 
      tttctggga aaaccacta gatttgcat tttgataag 
      acgtttgca agactgacc gtacattag ttctagttt 
      gctacttgt gttttctat gtttttcaa taaagttaa 
      tttggtgtt tcttgcttt attacatat ctgatacaa 
      tcataatca tctaaatac aaataagag ataactatt 
      tattcagac tatttatta tcgccttat caagtgacg 
      tagctgatg tccatttag aatccaatc atatctacc 
      acgcaacgc aactgttga ctctctact aactggcag 
      aggcgttgc aaaacgact aacggactg ttgtaccac 
      gaaaaactg ccaaaaact agtaaaatt actgtattc 
      aatggtgtc aaaacggtt tggtttgtc gtttgttgc 
      accacttat taggtcgtc cattgtaca taccttgag 
      caaaaaagt gatgttttc gtagtacaa agtttaaaa 
      atgtcactt ttctactct gtttcgact agtttaaga 
      tatattgac actgttaca ctcttcttc acctcgact 
      cttagataa tataagata cagggtctt gctgaccaa 
      tgttatgtc tttgttaac actgagtta cttagaata 
      gttaggaaa cttcaaatt gttgagcag tttcattat 
      atttttata aatattttg tacaaaagt ctgacgagt 
      accaaatag agcaactcg ctttctatt tggttcaat 
      ttgatgcac gataattac aaatcataa ggctttagg 
      aacattcat aatcataaa acggcattt aaactgtaa 
      gttctagtg tagtggaat ggattatag gtactgtca 
      taataaaca ctatcagga att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY