Status
Please wait ...
Gene information
General information
TC034861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3695989..3704693
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034861
    iBeetle screen iB_06300

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034861 RA
      MAPSFSKYV TRNNALAAA GASTALLWI LISRRRKNL 
      YKLDRRKVR KTVDEEVKY FISEKKENR IKAQVDRQF 
      LKQLNHLLR IIIPGWTSP EAGFLFLVA ASLVARSVC 
      DLWMIDNGT KIESAIVSM DKSLFKKRL IKFIVALPL 
      IAVVNNTLK FSIGELKLR LRTNMTRRL YEEYLKNFT 
      YYRMSNLDN RISNADQLL TTDVDKFCE SVSELYCNT 
      AKPLLDIGI YVYKLSSTL GGSTPALML GYLFVSGAV 
      LTHLRKPTA RLTAGEQKL EGEFRHINS RLITHSEEI 
      AFYNGNARE KATLLSSYN KLLNHLRKF LRFRVFMGV 
      IDNLVAKYF ASVVGFWAV SLPFLSQGH TLAGSQHGE 
      RSRLYYTYG RMLVKLAEA IGRLVLAGR ELTRLAGFT 
      ARVTQLRQV LDELNQGNY ERTMVTGAE NLKANGGKF 
      IFQNNVIKF DRVPLITPN GDVLIKEIT FEIKSGMNV 
      LVCGPNGAG KSSLFRVLG ELWPLFGGE LTKPPRGKL 
      FYIPQRPYM TLGCLRDQL TYPHSGSEA LRRGTSDKQ 
      LEEHLHRVQ LGYLLEREG GLDAIADWL DVLSGGEKQ 
      RIAMARLFY HKPQFAILD ECTSAVSVD VEGSMYKYC 
      REVGITLLT VSHRKSLWQ HHEYVLHLD GRGAYSFRP 
      IEDVTEQFG S
    CDS sequence
    • >TC034861 RA
      atggcaccc agttttagc aaatatgtg acaagaaac 
      aatgctctt gcagccgct ggagcatcg acagcttta 
      ttatggatt ttaatatca cgacgaaga aaaaatttg 
      tacaaactg gacaggaga aaagtcagg aaaaccgtg 
      gatgaagaa gtgaaatat tttatatct gagaagaaa 
      gaaaatagg attaaggcg caagttgat aggcaattt 
      cttaagcaa cttaatcac ctacttaga ataattatc 
      ccaggatgg acttcacct gaggcggga tttctattc 
      cttgtagct gcgagttta gtggctaga tctgtgtgt 
      gatttatgg atgatagat aatggaacg aaaatagaa 
      agtgctata gtttcaatg gacaagagt cttttcaaa 
      aagcgcctt ataaaattc atcgtggcc cttccatta 
      attgcagtg gtaaacaac acgctcaaa ttttcaatt 
      ggcgaacta aaactaaga cttcgcacg aatatgaca 
      cgtcgtctt tacgaagag tacctcaaa aacttcacc 
      tattaccgc atgtccaac ctagacaat cgcatcagc 
      aacgccgac caacttcta accaccgac gtcgataaa 
      ttctgcgaa agcgtatca gagctttat tgcaacact 
      gccaaacca ctcctcgac attggcatc tacgtctat 
      aaattatcc tcaactctg ggcggaagc acgccggct 
      ttgatgctt ggttattta ttcgtttct ggggctgtc 
      ttaacccac ttgaggaaa cccacagct agactaaca 
      gccggcgaa cagaaacta gaaggcgag ttcagacat 
      atcaactca cgattgata acgcattct gaggagata 
      gccttctac aatgggaac gcgcgcgaa aaggcgacc 
      ttgttgtct agctataac aaactgtta aaccatttg 
      aggaagttt ctccggttt agggtcttt atgggcgtt 
      attgataat ctcgtagcg aaatatttc gcaagtgtt 
      gtcgggttc tgggctgtg tctttacca tttttatcg 
      caaggacat acacttgcc ggttcgcaa catggcgaa 
      aggtctcga ttgtattac acttatggg cgcatgttg 
      gtaaaactg gcagaagct ataggtcgt ttggtccta 
      gcaggccgt gagttgacc agattggct ggttttaca 
      gcacgcgtg acgcaacta agacaagtg ctagatgaa 
      ctaaatcaa gggaattac gaacgtacg atggtgaca 
      ggagccgaa aatttgaag gcaaacgga ggcaagttc 
      atcttccag aacaatgtg attaagttt gaccgggtt 
      cctttgatt actcctaat ggtgatgtt ttgatcaaa 
      gagatcact tttgagatc aaatctgga atgaatgtc 
      ttagtttgt gggcctaac ggagctggg aaatcgtcc 
      ctgtttcgc gttttgggc gaattgtgg cctttattt 
      ggcggtgaa ttgacgaaa ccgccacgt ggaaaacta 
      ttctatatc ccgcaaagg ccctacatg actttgggt 
      tgcttgcgc gatcagttg acttatcca cattcgggt 
      tcggaggct ttgaggcgt ggaaccagt gataaacaa 
      ttggaggaa cacctgcat agagttcag ttggggtat 
      ttgttggag cgcgaagga ggcttggac gccattgct 
      gattggctg gatgtgttg agtggaggg gagaaacaa 
      cgtatagcg atggcgcga ttgttttat cataagcca 
      cagtttgcg attttggat gaatgcact agtgctgtt 
      tcggtggat gttgaagga agcatgtac aagtattgt 
      agagaagtt ggtataaca ctgttgaca gtgtcacat 
      aggaagtca ttatggcaa catcacgaa tatgtttta 
      catttggat gggagagga gcatactct ttccggcct 
      attgaagat gtaacagag caatttggg tcttaa
    mRNA sequence
    • >TC034861 RA
      catgacaga acggccatt tgtcaatta tcacaaaat 
      ggaaaactt tctaatttt cctttaatg aatcctttc 
      gattttaac gatcggtta atgcattcc acaggttcg 
      gccaaaatc atgtgatcg gtcgaagct ccaatgaca 
      gtcgctgac tggagctca gtaaaatac ttggtaaag 
      tctgcttaa acgttaggt gtcagtgtt tgtggatga 
      gtgagtgga aacaaatac aattgatcg gaaaactgc 
      ttggtcacg atcactcaa tacttcttg tgaaaagtg 
      cgaaaagat gaagtaaaa tggcaccca gttttagca 
      aatatgtga caagaaaca atgctcttg cagccgctg 
      gagcatcga cagctttat tatggattt taatatcac 
      gacgaagaa aaaatttgt acaaactgg acaggagaa 
      aagtcagga aaaccgtgg atgaagaag tgaaatatt 
      ttatatctg agaagaaag aaaatagga ttaaggcgc 
      aagttgata ggcaatttc ttaagcaac ttaatcacc 
      tacttagaa taattatcc caggatgga cttcacctg 
      aggcgggat ttctattcc ttgtagctg cgagtttag 
      tggctagat ctgtgtgtg atttatgga tgatagata 
      atggaacga aaatagaaa gtgctatag tttcaatgg 
      acaagagtc ttttcaaaa agcgcctta taaaattca 
      tcgtggccc ttccattaa ttgcagtgg taaacaaca 
      cgctcaaat tttcaattg gcgaactaa aactaagac 
      ttcgcacga atatgacac gtcgtcttt acgaagagt 
      acctcaaaa acttcacct attaccgca tgtccaacc 
      tagacaatc gcatcagca acgccgacc aacttctaa 
      ccaccgacg tcgataaat tctgcgaaa gcgtatcag 
      agctttatt gcaacactg ccaaaccac tcctcgaca 
      ttggcatct acgtctata aattatcct caactctgg 
      gcggaagca cgccggctt tgatgcttg gttatttat 
      tcgtttctg gggctgtct taacccact tgaggaaac 
      ccacagcta gactaacag ccggcgaac agaaactag 
      aaggcgagt tcagacata tcaactcac gattgataa 
      cgcattctg aggagatag ccttctaca atgggaacg 
      cgcgcgaaa aggcgacct tgttgtcta gctataaca 
      aactgttaa accatttga ggaagtttc tccggttta 
      gggtcttta tgggcgtta ttgataatc tcgtagcga 
      aatatttcg caagtgttg tcgggttct gggctgtgt 
      ctttaccat ttttatcgc aaggacata cacttgccg 
      gttcgcaac atggcgaaa ggtctcgat tgtattaca 
      cttatgggc gcatgttgg taaaactgg cagaagcta 
      taggtcgtt tggtcctag caggccgtg agttgacca 
      gattggctg gttttacag cacgcgtga cgcaactaa 
      gacaagtgc tagatgaac taaatcaag ggaattacg 
      aacgtacga tggtgacag gagccgaaa atttgaagg 
      caaacggag gcaagttca tcttccaga acaatgtga 
      ttaagtttg accgggttc ctttgatta ctcctaatg 
      gtgatgttt tgatcaaag agatcactt ttgagatca 
      aatctggaa tgaatgtct tagtttgtg ggcctaacg 
      gagctggga aatcgtccc tgtttcgcg ttttgggcg 
      aattgtggc ctttatttg gcggtgaat tgacgaaac 
      cgccacgtg gaaaactat tctatatcc cgcaaaggc 
      cctacatga ctttgggtt gcttgcgcg atcagttga 
      cttatccac attcgggtt cggaggctt tgaggcgtg 
      gaaccagtg ataaacaat tggaggaac acctgcata 
      gagttcagt tggggtatt tgttggagc gcgaaggag 
      gcttggacg ccattgctg attggctgg atgtgttga 
      gtggagggg agaaacaac gtatagcga tggcgcgat 
      tgttttatc ataagccac agtttgcga ttttggatg 
      aatgcacta gtgctgttt cggtggatg ttgaaggaa 
      gcatgtaca agtattgta gagaagttg gtataacac 
      tgttgacag tgtcacata ggaagtcat tatggcaac 
      atcacgaat atgttttac atttggatg ggagaggag 
      catactctt tccggccta ttgaagatg taacagagc 
      aatttgggt cttaattag ctttaaaat ctaggaaaa 
      tttatacaa taactaaaa cagggggac atgacaaat 
      gcggtttcc cattggaaa tattgaaga aaccgcatt 
      ttttgttca tggcctact tttgatgta aaagaacgt 
      atatcaaga aatgaaatg atatgtcaa aacctatgt 
      gatcgatag ccgcagctt ccttgataa atgaaattc 
      ttgttacta taattacac aattaagtg tgtttttgt 
      gttagtaaa cttctgcgc tcacatcac aaccatagc 
      tgctgaaaa aattgtttt cctctacag tggcttctt 
      cacagaaga aaaaatgga taacctact ttactgatc 
      gaaaaagct gtgaaagtg acttttttt taagcttac 
      gttacgtta aatttgaat tgtttgacg ccaatatca 
      tcatcaaaa atcccatca atcaacatg aaaaaaatc 
      ctttataaa aaataaaaa cagtactat att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY