General information | |||
TC034861 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3695989..3704693 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034861 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06300 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC034861 RA
MAPSFSKYV TRNNALAAA GASTALLWI LISRRRKNL YKLDRRKVR KTVDEEVKY FISEKKENR IKAQVDRQF LKQLNHLLR IIIPGWTSP EAGFLFLVA ASLVARSVC DLWMIDNGT KIESAIVSM DKSLFKKRL IKFIVALPL IAVVNNTLK FSIGELKLR LRTNMTRRL YEEYLKNFT YYRMSNLDN RISNADQLL TTDVDKFCE SVSELYCNT AKPLLDIGI YVYKLSSTL GGSTPALML GYLFVSGAV LTHLRKPTA RLTAGEQKL EGEFRHINS RLITHSEEI AFYNGNARE KATLLSSYN KLLNHLRKF LRFRVFMGV IDNLVAKYF ASVVGFWAV SLPFLSQGH TLAGSQHGE RSRLYYTYG RMLVKLAEA IGRLVLAGR ELTRLAGFT ARVTQLRQV LDELNQGNY ERTMVTGAE NLKANGGKF IFQNNVIKF DRVPLITPN GDVLIKEIT FEIKSGMNV LVCGPNGAG KSSLFRVLG ELWPLFGGE LTKPPRGKL FYIPQRPYM TLGCLRDQL TYPHSGSEA LRRGTSDKQ LEEHLHRVQ LGYLLEREG GLDAIADWL DVLSGGEKQ RIAMARLFY HKPQFAILD ECTSAVSVD VEGSMYKYC REVGITLLT VSHRKSLWQ HHEYVLHLD GRGAYSFRP IEDVTEQFG S
- >TC034861 RA
atggcaccc agttttagc aaatatgtg acaagaaac aatgctctt gcagccgct ggagcatcg acagcttta ttatggatt ttaatatca cgacgaaga aaaaatttg tacaaactg gacaggaga aaagtcagg aaaaccgtg gatgaagaa gtgaaatat tttatatct gagaagaaa gaaaatagg attaaggcg caagttgat aggcaattt cttaagcaa cttaatcac ctacttaga ataattatc ccaggatgg acttcacct gaggcggga tttctattc cttgtagct gcgagttta gtggctaga tctgtgtgt gatttatgg atgatagat aatggaacg aaaatagaa agtgctata gtttcaatg gacaagagt cttttcaaa aagcgcctt ataaaattc atcgtggcc cttccatta attgcagtg gtaaacaac acgctcaaa ttttcaatt ggcgaacta aaactaaga cttcgcacg aatatgaca cgtcgtctt tacgaagag tacctcaaa aacttcacc tattaccgc atgtccaac ctagacaat cgcatcagc aacgccgac caacttcta accaccgac gtcgataaa ttctgcgaa agcgtatca gagctttat tgcaacact gccaaacca ctcctcgac attggcatc tacgtctat aaattatcc tcaactctg ggcggaagc acgccggct ttgatgctt ggttattta ttcgtttct ggggctgtc ttaacccac ttgaggaaa cccacagct agactaaca gccggcgaa cagaaacta gaaggcgag ttcagacat atcaactca cgattgata acgcattct gaggagata gccttctac aatgggaac gcgcgcgaa aaggcgacc ttgttgtct agctataac aaactgtta aaccatttg aggaagttt ctccggttt agggtcttt atgggcgtt attgataat ctcgtagcg aaatatttc gcaagtgtt gtcgggttc tgggctgtg tctttacca tttttatcg caaggacat acacttgcc ggttcgcaa catggcgaa aggtctcga ttgtattac acttatggg cgcatgttg gtaaaactg gcagaagct ataggtcgt ttggtccta gcaggccgt gagttgacc agattggct ggttttaca gcacgcgtg acgcaacta agacaagtg ctagatgaa ctaaatcaa gggaattac gaacgtacg atggtgaca ggagccgaa aatttgaag gcaaacgga ggcaagttc atcttccag aacaatgtg attaagttt gaccgggtt cctttgatt actcctaat ggtgatgtt ttgatcaaa gagatcact tttgagatc aaatctgga atgaatgtc ttagtttgt gggcctaac ggagctggg aaatcgtcc ctgtttcgc gttttgggc gaattgtgg cctttattt ggcggtgaa ttgacgaaa ccgccacgt ggaaaacta ttctatatc ccgcaaagg ccctacatg actttgggt tgcttgcgc gatcagttg acttatcca cattcgggt tcggaggct ttgaggcgt ggaaccagt gataaacaa ttggaggaa cacctgcat agagttcag ttggggtat ttgttggag cgcgaagga ggcttggac gccattgct gattggctg gatgtgttg agtggaggg gagaaacaa cgtatagcg atggcgcga ttgttttat cataagcca cagtttgcg attttggat gaatgcact agtgctgtt tcggtggat gttgaagga agcatgtac aagtattgt agagaagtt ggtataaca ctgttgaca gtgtcacat aggaagtca ttatggcaa catcacgaa tatgtttta catttggat gggagagga gcatactct ttccggcct attgaagat gtaacagag caatttggg tcttaa
- >TC034861 RA
catgacaga acggccatt tgtcaatta tcacaaaat ggaaaactt tctaatttt cctttaatg aatcctttc gattttaac gatcggtta atgcattcc acaggttcg gccaaaatc atgtgatcg gtcgaagct ccaatgaca gtcgctgac tggagctca gtaaaatac ttggtaaag tctgcttaa acgttaggt gtcagtgtt tgtggatga gtgagtgga aacaaatac aattgatcg gaaaactgc ttggtcacg atcactcaa tacttcttg tgaaaagtg cgaaaagat gaagtaaaa tggcaccca gttttagca aatatgtga caagaaaca atgctcttg cagccgctg gagcatcga cagctttat tatggattt taatatcac gacgaagaa aaaatttgt acaaactgg acaggagaa aagtcagga aaaccgtgg atgaagaag tgaaatatt ttatatctg agaagaaag aaaatagga ttaaggcgc aagttgata ggcaatttc ttaagcaac ttaatcacc tacttagaa taattatcc caggatgga cttcacctg aggcgggat ttctattcc ttgtagctg cgagtttag tggctagat ctgtgtgtg atttatgga tgatagata atggaacga aaatagaaa gtgctatag tttcaatgg acaagagtc ttttcaaaa agcgcctta taaaattca tcgtggccc ttccattaa ttgcagtgg taaacaaca cgctcaaat tttcaattg gcgaactaa aactaagac ttcgcacga atatgacac gtcgtcttt acgaagagt acctcaaaa acttcacct attaccgca tgtccaacc tagacaatc gcatcagca acgccgacc aacttctaa ccaccgacg tcgataaat tctgcgaaa gcgtatcag agctttatt gcaacactg ccaaaccac tcctcgaca ttggcatct acgtctata aattatcct caactctgg gcggaagca cgccggctt tgatgcttg gttatttat tcgtttctg gggctgtct taacccact tgaggaaac ccacagcta gactaacag ccggcgaac agaaactag aaggcgagt tcagacata tcaactcac gattgataa cgcattctg aggagatag ccttctaca atgggaacg cgcgcgaaa aggcgacct tgttgtcta gctataaca aactgttaa accatttga ggaagtttc tccggttta gggtcttta tgggcgtta ttgataatc tcgtagcga aatatttcg caagtgttg tcgggttct gggctgtgt ctttaccat ttttatcgc aaggacata cacttgccg gttcgcaac atggcgaaa ggtctcgat tgtattaca cttatgggc gcatgttgg taaaactgg cagaagcta taggtcgtt tggtcctag caggccgtg agttgacca gattggctg gttttacag cacgcgtga cgcaactaa gacaagtgc tagatgaac taaatcaag ggaattacg aacgtacga tggtgacag gagccgaaa atttgaagg caaacggag gcaagttca tcttccaga acaatgtga ttaagtttg accgggttc ctttgatta ctcctaatg gtgatgttt tgatcaaag agatcactt ttgagatca aatctggaa tgaatgtct tagtttgtg ggcctaacg gagctggga aatcgtccc tgtttcgcg ttttgggcg aattgtggc ctttatttg gcggtgaat tgacgaaac cgccacgtg gaaaactat tctatatcc cgcaaaggc cctacatga ctttgggtt gcttgcgcg atcagttga cttatccac attcgggtt cggaggctt tgaggcgtg gaaccagtg ataaacaat tggaggaac acctgcata gagttcagt tggggtatt tgttggagc gcgaaggag gcttggacg ccattgctg attggctgg atgtgttga gtggagggg agaaacaac gtatagcga tggcgcgat tgttttatc ataagccac agtttgcga ttttggatg aatgcacta gtgctgttt cggtggatg ttgaaggaa gcatgtaca agtattgta gagaagttg gtataacac tgttgacag tgtcacata ggaagtcat tatggcaac atcacgaat atgttttac atttggatg ggagaggag catactctt tccggccta ttgaagatg taacagagc aatttgggt cttaattag ctttaaaat ctaggaaaa tttatacaa taactaaaa cagggggac atgacaaat gcggtttcc cattggaaa tattgaaga aaccgcatt ttttgttca tggcctact tttgatgta aaagaacgt atatcaaga aatgaaatg atatgtcaa aacctatgt gatcgatag ccgcagctt ccttgataa atgaaattc ttgttacta taattacac aattaagtg tgtttttgt gttagtaaa cttctgcgc tcacatcac aaccatagc tgctgaaaa aattgtttt cctctacag tggcttctt cacagaaga aaaaatgga taacctact ttactgatc gaaaaagct gtgaaagtg acttttttt taagcttac gttacgtta aatttgaat tgtttgacg ccaatatca tcatcaaaa atcccatca atcaacatg aaaaaaatc ctttataaa aaataaaaa cagtactat att
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
