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Gene information
General information
TC011992 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9131952..9140909
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011992
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06297

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber early not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber late degenerated - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 4

    tropharium potentiallystructure irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    Pupal morphology
    elytra shortened - Number of animals showing the phenotype: 5

    elytra not closed - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011992 RA
      MSSTGMVLE PPSKPHNNY RPHHNNTNS KRKSKHDRS 
      GRKRSKSFS GCGLMMDMK PVPPTKFLL GGNINDPLN 
      LNSLQDEDI NRAMNAITP KSSPIPTPP RRKGQIEVI 
      IPPNINDPL NLIGCADDA EYEQQLYSP VKKGRKKRL 
      KKRRTVSAS DGDVEARTP ETSTDSAKI PVEVEVEKD 
      LSLELSPKK EKSKRKVEE HGATQAKKF KYSMDKIVS 
      PVVPQPGTW LKRSNHKKR PRQKRDTTE AMPQFKEKD 
      KQYQYGNYN RYYGYRNPH SEVDNRLRL FHQHRYLFE 
      GKDILDIGC NVGHVTLSV ARDFGAKSV TGIDIDPKL 
      ISIARKNVK YYVKNSDSP KSERVGEVP VNKKSSEFF 
      PISMPILYG PIDIPGFND GQTGRDFPN NVVFKTCNY 
      VLDDDSLLA LEQPQFDVI LCLSITKWI HLNWGDSGM 
      KQAFRRMYA QLRPGGKLI LEPQNWASY KSKRKLTET 
      TFNNYNSIE FFPEKFTEY LLSSVVGFA KSEILGFPY 
      NHSKGFRRP IQIFTKSTM FPSERIEAT PNNSITPNN 
      DDPLYQKLL KTEKYESQT QPLVEKSEH IYYTNILNR 
      YCGNSEENF EVNKQIEDD LNKEQLDEK SQSEMQDVS 
      GDNT
    • >TC011992 RB
      MSSTGMVLE PPSKPHNNY RPHHNNTNS KRKSKHDRS 
      GRKRSKSFS GCGLMMDMK PVPPTKFLL GGNINDPLN 
      LNSLQDEDI NRAMNAITP KSSPIPTPP RRKGQIEVI 
      IPPNINDPL NLIGCADDA EYEQQLYSP VKKGRKKRL 
      KKRRTVSAS DGDVEARTP ETSTDSAKI PVEVEVEKD 
      LSLELSPKK EKSKRKVEE HGATQAKKF KYSMDKIVS 
      PVVPQPGTW LKRSNHKKR PRQKRDTTE AMPQFKEKD 
      KQYQYGNYN RYYGYRNPH SEVDNRLRL FHQHRYLFE 
      GKDILDIGC NVGHVTLSV ARDFGAKSV TGIDIDPKL 
      ISIARKNVK YYVKNSDSP KSERVGEVP VNKKSSFND 
      GQTGRDFPN NVVFKTCNY VLDDDSLLA LEQPQFDVI 
      LCLSITKWI HLNWGDSGM KQAFRRMYA QLRPGGKLI 
      LEPQNWASY KSKRKLTET TFNNYNSIE FFPEKFTEY 
      LLSSVVGFA KSEILGFPY NHSKGFRRP IQIFTKSTM 
      FPSERIEAT PNNSITPNN DDPLYQKLL KTEKYESQT 
      QPLVEKSEH IYYTNILNR YCGNSEENF EVNKQIEDD 
      LNKEQLDEK SQSEMQDVS GDNT
    CDS sequence
    • >TC011992 RA
      atgtcatcg accggcatg gtcctcgaa cccccgtca 
      aaaccccac aacaattac cgcccccat cacaacaac 
      accaactcg aaacgcaag tcgaaacat gaccgctcc 
      ggccgcaaa cgctcgaaa agtttctcc ggctgtggc 
      ctgatgatg gacatgaag ccggtgcct ccgaccaaa 
      ttcctcctc gggggcaac atcaacgac cccctcaat 
      ctcaacagc ctccaagac gaggatata aatcgggcc 
      atgaacgcc atcaccccc aagtcctcc cccatcccc 
      acgccaccg aggcggaag gggcagatc gaggtgatc 
      atccctccc aatatcaac gatcccctg aatctgatt 
      gggtgcgcc gacgatgcg gagtacgaa caacagttg 
      tattcgcca gtgaagaaa gggaggaag aaacgcttg 
      aagaagaga agaacggtg tcggcgtcc gatggagat 
      gtggaagcg aggactccg gaaacgtcc acggattcg 
      gcgaagatt cccgttgaa gtggaagtc gaaaaggat 
      ctcagtctc gaattgtcg ccaaagaag gagaagtcg 
      aagcggaaa gtggaggaa catggggcg acgcaagcg 
      aagaagttt aagtattcg atggataag attgtcagt 
      ccagtggtg ccacaaccg gggacgtgg ttgaagagg 
      tcgaatcat aagaagaga ccgaggcag aagagggat 
      accaccgaa gcgatgcca cagttcaag gagaaggat 
      aagcagtac cagtatgga aattataat aggtactac 
      gggtatcgc aacccccac agtgaagta gacaataga 
      ctgagactc tttcaccaa caccgctat ttattcgaa 
      gggaaagac atactagac ataggttgt aacgtgggt 
      cacgtgacg ttatccgta gcgcgcgat ttcggtgct 
      aaaagtgtg acaggcata gacatagat ccgaaatta 
      atatcaata gcaagaaag aacgtaaaa tattacgtt 
      aaaaattca gattcgccg aaatctgag agggtcggt 
      gaagtaccg gtgaataag aaaagtagt gagtttttc 
      cctatttct atgcccata ttatatgga cccattgac 
      attccaggc tttaatgac gggcaaacc ggacgcgat 
      tttcccaat aatgtcgtt tttaaaacg tgtaattat 
      gttttggac gacgactca ctcctggct cttgagcag 
      ccacagttc gacgttatt ttatgtcta agcatcacc 
      aagtggatt cacttgaac tggggggat tcggggatg 
      aaacaggcc tttagaagg atgtacgcc cagttgagg 
      ccgggcggg aaattaatc ctcgaacct caaaactgg 
      gcgagttat aaatctaag cgaaaattg acggaaaca 
      acttttaat aattacaat tcgatcgaa tttttcccg 
      gagaaattc accgagtac ctgctgtcg tcagtagtg 
      ggtttcgcc aagagtgaa attctaggt tttccctac 
      aaccattcg aaaggtttc cgaaggcca atccagatt 
      tttacgaaa agtacgatg tttcccagc gaacggatc 
      gaagcaaca ccaaataat agcatcaca ccaaacaat 
      gatgatcct ctctatcag aaattacta aagactgaa 
      aagtacgaa tctcagacg cagccgctt gtcgagaag 
      tcggaacat atttattat acgaatatt ttaaataga 
      tattgcggt aattccgaa gagaacttt gaagttaat 
      aagcagatc gaagacgat ttgaacaaa gaacaactg 
      gacgagaag agtcagagt gagatgcag gatgtttcg 
      ggggataat acttaa
    • >TC011992 RB
      atgtcatcg accggcatg gtcctcgaa cccccgtca 
      aaaccccac aacaattac cgcccccat cacaacaac 
      accaactcg aaacgcaag tcgaaacat gaccgctcc 
      ggccgcaaa cgctcgaaa agtttctcc ggctgtggc 
      ctgatgatg gacatgaag ccggtgcct ccgaccaaa 
      ttcctcctc gggggcaac atcaacgac cccctcaat 
      ctcaacagc ctccaagac gaggatata aatcgggcc 
      atgaacgcc atcaccccc aagtcctcc cccatcccc 
      acgccaccg aggcggaag gggcagatc gaggtgatc 
      atccctccc aatatcaac gatcccctg aatctgatt 
      gggtgcgcc gacgatgcg gagtacgaa caacagttg 
      tattcgcca gtgaagaaa gggaggaag aaacgcttg 
      aagaagaga agaacggtg tcggcgtcc gatggagat 
      gtggaagcg aggactccg gaaacgtcc acggattcg 
      gcgaagatt cccgttgaa gtggaagtc gaaaaggat 
      ctcagtctc gaattgtcg ccaaagaag gagaagtcg 
      aagcggaaa gtggaggaa catggggcg acgcaagcg 
      aagaagttt aagtattcg atggataag attgtcagt 
      ccagtggtg ccacaaccg gggacgtgg ttgaagagg 
      tcgaatcat aagaagaga ccgaggcag aagagggat 
      accaccgaa gcgatgcca cagttcaag gagaaggat 
      aagcagtac cagtatgga aattataat aggtactac 
      gggtatcgc aacccccac agtgaagta gacaataga 
      ctgagactc tttcaccaa caccgctat ttattcgaa 
      gggaaagac atactagac ataggttgt aacgtgggt 
      cacgtgacg ttatccgta gcgcgcgat ttcggtgct 
      aaaagtgtg acaggcata gacatagat ccgaaatta 
      atatcaata gcaagaaag aacgtaaaa tattacgtt 
      aaaaattca gattcgccg aaatctgag agggtcggt 
      gaagtaccg gtgaataag aaaagtagc tttaatgac 
      gggcaaacc ggacgcgat tttcccaat aatgtcgtt 
      tttaaaacg tgtaattat gttttggac gacgactca 
      ctcctggct cttgagcag ccacagttc gacgttatt 
      ttatgtcta agcatcacc aagtggatt cacttgaac 
      tggggggat tcggggatg aaacaggcc tttagaagg 
      atgtacgcc cagttgagg ccgggcggg aaattaatc 
      ctcgaacct caaaactgg gcgagttat aaatctaag 
      cgaaaattg acggaaaca acttttaat aattacaat 
      tcgatcgaa tttttcccg gagaaattc accgagtac 
      ctgctgtcg tcagtagtg ggtttcgcc aagagtgaa 
      attctaggt tttccctac aaccattcg aaaggtttc 
      cgaaggcca atccagatt tttacgaaa agtacgatg 
      tttcccagc gaacggatc gaagcaaca ccaaataat 
      agcatcaca ccaaacaat gatgatcct ctctatcag 
      aaattacta aagactgaa aagtacgaa tctcagacg 
      cagccgctt gtcgagaag tcggaacat atttattat 
      acgaatatt ttaaataga tattgcggt aattccgaa 
      gagaacttt gaagttaat aagcagatc gaagacgat 
      ttgaacaaa gaacaactg gacgagaag agtcagagt 
      gagatgcag gatgtttcg ggggataat acttaa
    mRNA sequence
    • >TC011992 RA
      caaactccg gcactgata ttaaaatgg agtctagtt 
      tacaagcac aacgatttg tagtcgttc agacggtgc 
      gttgcaaag tgaagtctc tttttcggc cgtttttca 
      gcggaacct aagggaaaa gtggccctc aaagggcat 
      caaacgaaa gctgaagcc acgccggac acgttgaag 
      gaaaaatgt catcgaccg gcatggtcc tcgaacccc 
      cgtcaaaac cccacaaca attaccgcc cccatcaca 
      acaacacca actcgaaac gcaagtcga aacatgacc 
      gctccggcc gcaaacgct cgaaaagtt tctccggct 
      gtggcctga tgatggaca tgaagccgg tgcctccga 
      ccaaattcc tcctcgggg gcaacatca acgaccccc 
      tcaatctca acagcctcc aagacgagg atataaatc 
      gggccatga acgccatca cccccaagt cctccccca 
      tccccacgc caccgaggc ggaaggggc agatcgagg 
      tgatcatcc ctcccaata tcaacgatc ccctgaatc 
      tgattgggt gcgccgacg atgcggagt acgaacaac 
      agttgtatt cgccagtga agaaaggga ggaagaaac 
      gcttgaaga agagaagaa cggtgtcgg cgtccgatg 
      gagatgtgg aagcgagga ctccggaaa cgtccacgg 
      attcggcga agattcccg ttgaagtgg aagtcgaaa 
      aggatctca gtctcgaat tgtcgccaa agaaggaga 
      agtcgaagc ggaaagtgg aggaacatg gggcgacgc 
      aagcgaaga agtttaagt attcgatgg ataagattg 
      tcagtccag tggtgccac aaccgggga cgtggttga 
      agaggtcga atcataaga agagaccga ggcagaaga 
      gggatacca ccgaagcga tgccacagt tcaaggaga 
      aggataagc agtaccagt atggaaatt ataataggt 
      actacgggt atcgcaacc cccacagtg aagtagaca 
      atagactga gactctttc accaacacc gctatttat 
      tcgaaggga aagacatac tagacatag gttgtaacg 
      tgggtcacg tgacgttat ccgtagcgc gcgatttcg 
      gtgctaaaa gtgtgacag gcatagaca tagatccga 
      aattaatat caatagcaa gaaagaacg taaaatatt 
      acgttaaaa attcagatt cgccgaaat ctgagaggg 
      tcggtgaag taccggtga ataagaaaa gtagtgagt 
      ttttcccta tttctatgc ccatattat atggaccca 
      ttgacattc caggcttta atgacgggc aaaccggac 
      gcgattttc ccaataatg tcgttttta aaacgtgta 
      attatgttt tggacgacg actcactcc tggctcttg 
      agcagccac agttcgacg ttattttat gtctaagca 
      tcaccaagt ggattcact tgaactggg gggattcgg 
      ggatgaaac aggccttta gaaggatgt acgcccagt 
      tgaggccgg gcgggaaat taatcctcg aacctcaaa 
      actgggcga gttataaat ctaagcgaa aattgacgg 
      aaacaactt ttaataatt acaattcga tcgaatttt 
      tcccggaga aattcaccg agtacctgc tgtcgtcag 
      tagtgggtt tcgccaaga gtgaaattc taggttttc 
      cctacaacc attcgaaag gtttccgaa ggccaatcc 
      agattttta cgaaaagta cgatgtttc ccagcgaac 
      ggatcgaag caacaccaa ataatagca tcacaccaa 
      acaatgatg atcctctct atcagaaat tactaaaga 
      ctgaaaagt acgaatctc agacgcagc cgcttgtcg 
      agaagtcgg aacatattt attatacga atattttaa 
      atagatatt gcggtaatt ccgaagaga actttgaag 
      ttaataagc agatcgaag acgatttga acaaagaac 
      aactggacg agaagagtc agagtgaga tgcaggatg 
      tttcggggg ataatactt aaaaagtgt taggagata 
      gagtttttt ttcgtactt gattttgta caaatcgtt 
      gactttttt taatgttcg attgtttgg cttatgtac 
      atatgtata tgcaag
    • >TC011992 RB
      caaactccg gcactgata ttaaaatgg agtctagtt 
      tacaagcac aacgatttg tagtcgttc agacggtgc 
      gttgcaaag tgaagtctc tttttcggc cgtttttca 
      gcggaacct aagggaaaa gtggccctc aaagggcat 
      caaacgaaa gctgaagcc acgccggac acgttgaag 
      gaaaaatgt catcgaccg gcatggtcc tcgaacccc 
      cgtcaaaac cccacaaca attaccgcc cccatcaca 
      acaacacca actcgaaac gcaagtcga aacatgacc 
      gctccggcc gcaaacgct cgaaaagtt tctccggct 
      gtggcctga tgatggaca tgaagccgg tgcctccga 
      ccaaattcc tcctcgggg gcaacatca acgaccccc 
      tcaatctca acagcctcc aagacgagg atataaatc 
      gggccatga acgccatca cccccaagt cctccccca 
      tccccacgc caccgaggc ggaaggggc agatcgagg 
      tgatcatcc ctcccaata tcaacgatc ccctgaatc 
      tgattgggt gcgccgacg atgcggagt acgaacaac 
      agttgtatt cgccagtga agaaaggga ggaagaaac 
      gcttgaaga agagaagaa cggtgtcgg cgtccgatg 
      gagatgtgg aagcgagga ctccggaaa cgtccacgg 
      attcggcga agattcccg ttgaagtgg aagtcgaaa 
      aggatctca gtctcgaat tgtcgccaa agaaggaga 
      agtcgaagc ggaaagtgg aggaacatg gggcgacgc 
      aagcgaaga agtttaagt attcgatgg ataagattg 
      tcagtccag tggtgccac aaccgggga cgtggttga 
      agaggtcga atcataaga agagaccga ggcagaaga 
      gggatacca ccgaagcga tgccacagt tcaaggaga 
      aggataagc agtaccagt atggaaatt ataataggt 
      actacgggt atcgcaacc cccacagtg aagtagaca 
      atagactga gactctttc accaacacc gctatttat 
      tcgaaggga aagacatac tagacatag gttgtaacg 
      tgggtcacg tgacgttat ccgtagcgc gcgatttcg 
      gtgctaaaa gtgtgacag gcatagaca tagatccga 
      aattaatat caatagcaa gaaagaacg taaaatatt 
      acgttaaaa attcagatt cgccgaaat ctgagaggg 
      tcggtgaag taccggtga ataagaaaa gtagcttta 
      atgacgggc aaaccggac gcgattttc ccaataatg 
      tcgttttta aaacgtgta attatgttt tggacgacg 
      actcactcc tggctcttg agcagccac agttcgacg 
      ttattttat gtctaagca tcaccaagt ggattcact 
      tgaactggg gggattcgg ggatgaaac aggccttta 
      gaaggatgt acgcccagt tgaggccgg gcgggaaat 
      taatcctcg aacctcaaa actgggcga gttataaat 
      ctaagcgaa aattgacgg aaacaactt ttaataatt 
      acaattcga tcgaatttt tcccggaga aattcaccg 
      agtacctgc tgtcgtcag tagtgggtt tcgccaaga 
      gtgaaattc taggttttc cctacaacc attcgaaag 
      gtttccgaa ggccaatcc agattttta cgaaaagta 
      cgatgtttc ccagcgaac ggatcgaag caacaccaa 
      ataatagca tcacaccaa acaatgatg atcctctct 
      atcagaaat tactaaaga ctgaaaagt acgaatctc 
      agacgcagc cgcttgtcg agaagtcgg aacatattt 
      attatacga atattttaa atagatatt gcggtaatt 
      ccgaagaga actttgaag ttaataagc agatcgaag 
      acgatttga acaaagaac aactggacg agaagagtc 
      agagtgaga tgcaggatg tttcggggg ataatactt 
      aaaaagtgt taggagata gagtttttt ttcgtactt 
      gattttgta caaatcgtt gactttttt taatgttcg 
      attgtttgg cttatgtac atatgtata tgcaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY