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Gene information
General information
TC011940 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11278022..11279112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC011940
      iBeetle screen iB_06295

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011940 RA
        MWEESSSGF GDESPILFK SAKRLRHVP DCPVSLEEI 
HKKLLLHLR KSQTMSQSE LRSFLKQTK GMDPPDCKG 
KWLLAKIRD LLNYSLKKR DFPKSVLSE SFTEAPKFS 
KGAGDVSFQ MPPSLLSNN DFMTPEKCN VKMEVDFSQ 
DLFSCSDNF ESLHGFSQE NYNIFKRVI EEEKVFKLP 
SVVSKIEDV LQVHDYCSS LLTQTITDS GQALEAQME 
DMGEDGANI FDNLTISNE ELTEDLEAE LEKTTTWVS 
EIIHNLKNL RVDEISNEE NAQVLNESS SSALSSKIT 
PYYSEENFK IVREACCTF KETLENRPN KAILKELLN 
NL
      CDS sequence
      • >TC011940 RA
        atgtgggag gagagttcg tccggtttt ggcgacgag 
tccccgatt ctcttcaaa tcggcgaag aggctccgc 
cacgtgccc gactgcccc gtttctctt gaagaaatc 
cacaaaaaa ctgctgctc catttgagg aaatcgcaa 
acgatgtcg caaagcgag ctgagatcg tttctgaag 
cagacgaaa gggatggac cccccggac tgcaagggc 
aagtggctg ctggcgaaa atcagggac ttgctgaac 
tacagcttg aaaaaacgt gacttccca aaatcggtc 
ttaagcgaa agtttcacc gaagccccc aagttcagc 
aaaggggca ggcgatgtt tccttccaa atgccccca 
agtctgctt tcgaataac gattttatg actcctgag 
aaatgcaac gtgaaaatg gaggttgat ttctcgcag 
gacttgttc tcgtgttcg gacaacttc gagtccttg 
cacggcttc tcgcaggaa aattacaac atatttaag 
agagttatt gaagaagag aaggtattt aagctgccc 
tcggttgtg agtaaaatc gaggatgtg ctgcaagtc 
cacgactat tgcagctct ttgctcacg cagaccata 
accgattct gggcaagcc ctggaggct cagatggaa 
gacatgggc gaagatggg gccaacatt tttgataac 
ttgaccata agtaacgaa gagttaaca gaagatttg 
gaggcggaa cttgaaaaa accacgacc tgggtttcg 
gaaattatt cacaatttg aagaacttg agagttgac 
gagataagt aacgaggaa aacgctcaa gtgctgaat 
gagagcagc agtagtgct ctaagctca aaaataacg 
ccttattac agcgaagaa aactttaag atagtgaga 
gaagcgtgt tgtacgttt aaggagacg ctcgaaaat 
aggcccaat aaggccatc ctgaaggaa ctactcaac 
aacttataa
      mRNA sequence
      • >TC011940 RA
        ttacaaatt caagcgtca aaattttta cggggcatt 
tttgaagtt caaattcaa aaaatcggt ttatttcga 
tttcgccaa tgtgggagg agagttcgt ccggttttg 
gcgacgagt ccccgattc tcttcaaat cggcgaaga 
ggctccgcc acgtgcccg actgccccg tttctcttg 
aagaaatcc acaaaaaac tgctgctcc atttgagga 
aatcgcaaa cgatgtcgc aaagcgagc tgagatcgt 
ttctgaagc agacgaaag ggatggacc ccccggact 
gcaagggca agtggctgc tggcgaaaa tcagggact 
tgctgaact acagcttga aaaaacgtg acttcccaa 
aatcggtct taagcgaaa gtttcaccg aagccccca 
agttcagca aaggggcag gcgatgttt ccttccaaa 
tgcccccaa gtctgcttt cgaataacg attttatga 
ctcctgaga aatgcaacg tgaaaatgg aggttgatt 
tctcgcagg acttgttct cgtgttcgg acaacttcg 
agtccttgc acggcttct cgcaggaaa attacaaca 
tatttaaga gagttattg aagaagaga aggtattta 
agctgccct cggttgtga gtaaaatcg aggatgtgc 
tgcaagtcc acgactatt gcagctctt tgctcacgc 
agaccataa ccgattctg ggcaagccc tggaggctc 
agatggaag acatgggcg aagatgggg ccaacattt 
ttgataact tgaccataa gtaacgaag agttaacag 
aagatttgg aggcggaac ttgaaaaaa ccacgacct 
gggtttcgg aaattattc acaatttga agaacttga 
gagttgacg agataagta acgaggaaa acgctcaag 
tgctgaatg agagcagca gtagtgctc taagctcaa 
aaataacgc cttattaca gcgaagaaa actttaaga 
tagtgagag aagcgtgtt gtacgttta aggagacgc 
tcgaaaata ggcccaata aggccatcc tgaaggaac 
tactcaaca acttataaa caaataaaa aataaagtc 
attctaatt tg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY