Status
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Gene information
General information
TC011842 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12676857..12683824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011842
    iBeetle screen iB_06294

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head seta partially irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    vertex triplet vertex triplet posterior potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially circumference irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 77.78% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011842 RA
      MEYTAENLE KAVTLFYRT EAGQQAEAH QWLTEAQNS 
      PQAWSFVWE LLSPHRNSE VQFFAATTL HTKLMKHWN 
      EVPEDHYEL LKKRILEAI INYAMGPKI VLNRLCITL 
      SAYIIHTVP THWPNAFEE LVSSFQPQH LPNVEPERV 
      IWILLEILT VIPEEFQST LLAVSQRMR VRTVLQDVS 
      KDILKVVEM CLMPLPSAG FDMLNLTTY LNAARCASA 
      WIQLGGLNI DDCTSVMNL LINLTCFAY WNKTDPECM 
      SPEEMELME VTVEALTTI IQHPHTNRY HNHVMKCSA 
      DMLYKFEKI LECERNSPE SNKDIVANL YGLLVTIAD 
      VHSKIFINN LKSENVDEQ RISFDFFNS ILKCTNLPG 
      LYPVDESSS TLTFGFWYT LQDDILSLE TAECAQLLL 
      MIKPYYRDL VCIMLRKSM FPLNEDGDW SLDDKEVFR 
      CYRQDIADT FIYCYNVLN LEMLDILNS KLIEALHKN 
      NTSVVPPPI QWNEVETCL HAFGAIAES IELENLYLP 
      KLMVTIKEI PFTDLHKKV MASALETVG SYSDWITEH 
      PEMLENVLP LVISALDKP EVATSATMA LKDLTHSCQ 
      KYLLPYADH ILLAAQSAL QGGALKLAE CSRLMYSIG 
      KVLSILPVP RIMDYLNII LAPSFKEMQ DLLNVEPSP 
      AVKTSLITR LKVLSSLFN SLCVKKSQT HIIEQPTVL 
      VMQNTMPLY KVIGEKYCT SGDVMEELS ILLKYVVTT 
      LLEDCTPLI NDILQLVVT VYRECPQSN ILMVAKTVV 
      IMFGHEEEF RAITQQLLH EIVSTTLQM CAQLNSANQ 
      LAEKTDVLE GFFAMMAQL IKKLPQVVF ASGIDTAAL 
      FQCAVLCLS LPETQTLKL CTSFLVNFI SQSRDTAQA 
      NIVQNYGES LVLRILINL GNTAPRSSV EIFSDLLLA 
      LNKKYCDNL SRWLNALLA QEDFPSPRI SRQQKENFI 
      KLVLREKAN KRKLSDSVL EFTLICRGI LREDPNMP
    CDS sequence
    • >TC011842 RA
      atggaatac acggcggaa aatttggaa aaagccgtg 
      accttattt taccgcacc gaagcgggg caacaggcg 
      gaggcccac cagtggctg accgaggcc cagaattcg 
      ccccaagcg tggagcttc gtgtgggag ttgttgagc 
      ccccatcgc aattccgaa gtgcagttc tttgctgcc 
      acgacgctc catacgaag ctgatgaag cactggaat 
      gaggtgccg gaggatcat tacgagctg ttgaagaag 
      cgcattctg gaggcgatt attaactat gccatgggc 
      cccaaaatt gtcctgaac cggctctgc attactttg 
      tcggcttat attatacac actgtgccg acgcattgg 
      cccaatgcg tttgaagag ctggtttcg agtttccag 
      ccccagcac ttgcccaac gttgagccc gaaagagtc 
      atttggata cttttagaa atcctgact gtgatacca 
      gaagagttt caaagcacg ttattagca gtaagccaa 
      cggatgagg gtacgcact gtcttgcaa gacgtttcg 
      aaagacatc cttaaagtg gtcgagatg tgtctcatg 
      ccacttcca agtgccggc ttcgatatg ttaaatttg 
      acgacgtat ttaaatgcg gcaagatgt gcgtctgct 
      tggatacag ttaggcggt ttaaatatt gacgattgt 
      acgtcagtt atgaatctt ttaataaat ttgacgtgt 
      ttcgcatat tggaacaag acggatcct gaatgcatg 
      tctccagaa gaaatggag ttgatggaa gtgactgtg 
      gaggctcta accaccata atccagcac ccgcacact 
      aatcggtat cacaaccat gtgatgaaa tgtagtgct 
      gatatgttg tacaaattt gaaaaaatc cttgaatgt 
      gaaaggaat agtccagag agcaataaa gacattgta 
      gccaacttg tatggctta ttggtaaca atagccgac 
      gtgcattcg aaaattttc atcaacaat ttaaaatcg 
      gaaaacgtg gacgagcaa cgaatcagt ttcgatttt 
      ttcaactcg attttaaaa tgtacaaat ctacccggc 
      ttgtacccc gttgatgaa tcaagcagt accttaact 
      ttcggtttt tggtacacg ttacaagac gatatttta 
      tcgctagaa acggccgaa tgcgcccaa ttgttacta 
      atgataaaa ccctactat cgcgactta gtttgtata 
      atgttacgc aaatccatg tttccattg aatgaggac 
      ggcgattgg tcgctggac gataaggaa gtgttccgc 
      tgttaccgg caagacatc gccgacacg ttcatctac 
      tgctacaat gttttaaat ttggaaatg ctcgatatt 
      ttgaacagt aaattgatc gaagctcta cataagaat 
      aatacatcg gtggttccc ccgcctatt caatggaac 
      gaagtcgag acttgcctt cacgccttc ggcgcaatt 
      gccgaatct atcgaactc gaaaattta tatttgccc 
      aagctgatg gttacgatt aaagaaatc ccctttacc 
      gacttgcac aaaaaagtc atggcttcg gcgctagaa 
      accgtaggc tcgtactct gattggatc accgaacat 
      ccagaaatg ttggaaaat gtgttacca ttggtgatt 
      tcggcgcta gacaaaccc gaagttgca accagcgct 
      accatggcc ttgaaagac ttgacacat agctgccag 
      aagtattta ttaccatac gccgaccat attctgcta 
      gcagcacaa tctgcgcta caagggggc gctttgaag 
      ttggccgaa tgtagccga cttatgtac agtatagga 
      aaggttttg agtattcta ccggtgcca cgtattatg 
      gactatctc aatataatt ctagcgcca tcttttaag 
      gaaatgcaa gacttgctg aatgtcgaa ccgtcaccc 
      gctgtgaaa acttcgcta attacgaga cttaaggtg 
      ttatcgtcg ttatttaac tcgctgtgt gttaagaag 
      agtcaaacg catattatc gaacagccg actgtctta 
      gttatgcaa aatacaatg cccttgtat aaggttata 
      ggggagaag tattgcacc agtggtgac gtcatggaa 
      gaactcagt attttattg aaatacgtt gtgacaacg 
      ttattggaa gactgcacc ccccttatt aacgacatc 
      ctccagctc gttgtgacg gtgtatcga gaatgcccc 
      cagtcgaat attttaatg gtcgctaaa acggtggtg 
      ataatgttt ggtcatgag gaagaattc cgcgctata 
      acgcagcaa cttttgcac gaaatagtc agcacaacg 
      ctgcaaatg tgcgcgcag ctgaacagt gccaatcag 
      ttagccgaa aaaacggac gtgcttgaa ggcttcttc 
      gcaatgatg gctcaacta atcaaaaag cttcctcaa 
      gtagtgttt gccagcggt atagacacc gctgcgttg 
      tttcagtgt gccgttttg tgtttgtct ctacccgag 
      actcagacg ttaaaattg tgtactagt ttcttagtg 
      aacttcata tcgcaaagt cgtgataca gctcaagct 
      aacattgtc caaaattac ggtgaaagt ctcgttttg 
      cgtattcta attaactta ggcaacacc gcgccgagg 
      tcgtccgta gagattttc agtgatttg ttacttgct 
      ttaaataaa aaatactgt gataatctt agtaggtgg 
      ttgaatgct ttgttggca caggaggac tttccctcg 
      ccgaggatt tccaggcaa caaaaggag aacttcatc 
      aaacttgtt ttgcgtgaa aaggcgaat aaacggaaa 
      ttgagcgac tcggttctg gaattcact ctcatttgt 
      cgagggata ttacgggag gatccaaac atgccgtag
    mRNA sequence
    • >TC011842 RA
      atcaataaa caccgtttt aaacaacac taaaaaaaa 
      tgttgcgct taaacaatt ttattaatt tttttccaa 
      aattgtaat ttttgggtt ggcgacagt gggggcgcc 
      ttaacctcg tccaattcg taaatcgac aatttgtgt 
      tttttatcg tggcaaaaa gtgcacaaa aacgcccca 
      tttcccagt tggtgtcag cccccttgg cccccagcg 
      cgtgtcgta agactgctg acgagcgcg tatttttga 
      cagttatcg acgtaacgc cgactgtca ctccgtaaa 
      atgtagcaa aagtaaaaa caaactcgt aattaattc 
      gaagatttg gtttaaatt cgcatcaag catggaata 
      cacggcgga aaatttgga aaaagccgt gaccttatt 
      ttaccgcac cgaagcggg gcaacaggc ggaggccca 
      ccagtggct gaccgaggc ccagaattc gccccaagc 
      gtggagctt cgtgtggga gttgttgag cccccatcg 
      caattccga agtgcagtt ctttgctgc cacgacgct 
      ccatacgaa gctgatgaa gcactggaa tgaggtgcc 
      ggaggatca ttacgagct gttgaagaa gcgcattct 
      ggaggcgat tattaacta tgccatggg ccccaaaat 
      tgtcctgaa ccggctctg cattacttt gtcggctta 
      tattataca cactgtgcc gacgcattg gcccaatgc 
      gtttgaaga gctggtttc gagtttcca gccccagca 
      cttgcccaa cgttgagcc cgaaagagt catttggat 
      acttttaga aatcctgac tgtgatacc agaagagtt 
      tcaaagcac gttattagc agtaagcca acggatgag 
      ggtacgcac tgtcttgca agacgtttc gaaagacat 
      ccttaaagt ggtcgagat gtgtctcat gccacttcc 
      aagtgccgg cttcgatat gttaaattt gacgacgta 
      tttaaatgc ggcaagatg tgcgtctgc ttggataca 
      gttaggcgg tttaaatat tgacgattg tacgtcagt 
      tatgaatct tttaataaa tttgacgtg tttcgcata 
      ttggaacaa gacggatcc tgaatgcat gtctccaga 
      agaaatgga gttgatgga agtgactgt ggaggctct 
      aaccaccat aatccagca cccgcacac taatcggta 
      tcacaacca tgtgatgaa atgtagtgc tgatatgtt 
      gtacaaatt tgaaaaaat ccttgaatg tgaaaggaa 
      tagtccaga gagcaataa agacattgt agccaactt 
      gtatggctt attggtaac aatagccga cgtgcattc 
      gaaaatttt catcaacaa tttaaaatc ggaaaacgt 
      ggacgagca acgaatcag tttcgattt tttcaactc 
      gattttaaa atgtacaaa tctacccgg cttgtaccc 
      cgttgatga atcaagcag taccttaac tttcggttt 
      ttggtacac gttacaaga cgatatttt atcgctaga 
      aacggccga atgcgccca attgttact aatgataaa 
      accctacta tcgcgactt agtttgtat aatgttacg 
      caaatccat gtttccatt gaatgagga cggcgattg 
      gtcgctgga cgataagga agtgttccg ctgttaccg 
      gcaagacat cgccgacac gttcatcta ctgctacaa 
      tgttttaaa tttggaaat gctcgatat tttgaacag 
      taaattgat cgaagctct acataagaa taatacatc 
      ggtggttcc cccgcctat tcaatggaa cgaagtcga 
      gacttgcct tcacgcctt cggcgcaat tgccgaatc 
      tatcgaact cgaaaattt atatttgcc caagctgat 
      ggttacgat taaagaaat cccctttac cgacttgca 
      caaaaaagt catggcttc ggcgctaga aaccgtagg 
      ctcgtactc tgattggat caccgaaca tccagaaat 
      gttggaaaa tgtgttacc attggtgat ttcggcgct 
      agacaaacc cgaagttgc aaccagcgc taccatggc 
      cttgaaaga cttgacaca tagctgcca gaagtattt 
      attaccata cgccgacca tattctgct agcagcaca 
      atctgcgct acaaggggg cgctttgaa gttggccga 
      atgtagccg acttatgta cagtatagg aaaggtttt 
      gagtattct accggtgcc acgtattat ggactatct 
      caatataat tctagcgcc atcttttaa ggaaatgca 
      agacttgct gaatgtcga accgtcacc cgctgtgaa 
      aacttcgct aattacgag acttaaggt gttatcgtc 
      gttatttaa ctcgctgtg tgttaagaa gagtcaaac 
      gcatattat cgaacagcc gactgtctt agttatgca 
      aaatacaat gcccttgta taaggttat aggggagaa 
      gtattgcac cagtggtga cgtcatgga agaactcag 
      tattttatt gaaatacgt tgtgacaac gttattgga 
      agactgcac cccccttat taacgacat cctccagct 
      cgttgtgac ggtgtatcg agaatgccc ccagtcgaa 
      tattttaat ggtcgctaa aacggtggt gataatgtt 
      tggtcatga ggaagaatt ccgcgctat aacgcagca 
      acttttgca cgaaatagt cagcacaac gctgcaaat 
      gtgcgcgca gctgaacag tgccaatca gttagccga 
      aaaaacgga cgtgcttga aggcttctt cgcaatgat 
      ggctcaact aatcaaaaa gcttcctca agtagtgtt 
      tgccagcgg tatagacac cgctgcgtt gtttcagtg 
      tgccgtttt gtgtttgtc tctacccga gactcagac 
      gttaaaatt gtgtactag tttcttagt gaacttcat 
      atcgcaaag tcgtgatac agctcaagc taacattgt 
      ccaaaatta cggtgaaag tctcgtttt gcgtattct 
      aattaactt aggcaacac cgcgccgag gtcgtccgt 
      agagatttt cagtgattt gttacttgc tttaaataa 
      aaaatactg tgataatct tagtaggtg gttgaatgc 
      tttgttggc acaggagga ctttccctc gccgaggat 
      ttccaggca acaaaagga gaacttcat caaacttgt 
      tttgcgtga aaaggcgaa taaacggaa attgagcga 
      ctcggttct ggaattcac tctcatttg tcgagggat 
      attacggga ggatccaaa catgccgta gttacgaaa 
      ataaggtaa gtgtttatt agagctaag ccaatcata 
      atgattgat tttgttcct gcaccccgt atttttcca 
      aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY