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Gene information
General information
TC011101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 372352..391299
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011101
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06289

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum potentially size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages partially potentiallyposition irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011101 RA
      MARYHLLKL GLAILVYFT RSMGCLETE ERKFNEEDL 
      VFYQGVTGK PGVDFPVLS HIPRTTFNC RNVDSGYYA 
      DLETDCQVF HICEEGKKI SFLCPNGTI FQQSELICE 
      WWFKVNCTD SPNFYEESA EQLREDNAR RKASRRVLQ 
      NHGAVMRTE EHSSVSASQ NGRKFNVGT TFQETNDLA 
      RAPSSDFVR DQKKKGRKP VQFQTQSIE ERKSFDDQK 
      VVPSKNHRA NERINQNGA VPQHQEVRN NSARSYNQF 
      GTTRQEVVP NNQRNGGRK VVQGSQDQN QKFETNAKG 
      FGTSVVPNR QEKEQHSNP NFQQYSNFH NQNSGFGTT 
      SDFKKMVPS QFNTQQSQQ ERKNIKTFG TKEVVSNQF 
      NNGQSAQNT FSNSTNREE EINPFFNNG FQDSNTNSA 
      ESTDKKSNV SNFSQSFSH RNFNVDEKR TTFSSSTTT 
      TPRSTTKVP PTSSNLQNH NQDYHQIKN LNIEGKGGT 
      IKQTSTVPL TRNDLVNRR LEPNDSDES QITQETSSF 
      YKNSFNRID QTIKTTSNP HPNHRSTYS EIKEPKFHS 
      TKAYTSLSS VTPQYLTTQ PINNGRPFV GSQVGTTLR 
      GFGTKPPTT TPVVPTEIF NVGRTDKSL KPIALSTLS 
      TFTPLPPLD LTVPSQGSF NTGTPSPKT ESSTRVIDH 
      ATVYGKFVK SGATTPTTG RTKILLSKS VFGTTPKTS 
      AEILSTSDS YNRGNSQVR FFGKVQTTT KIPGVQIIS 
      KSDGTTTKP AEILSTSNS YNRGNSQVQ FFGKVQTTA 
      NLNSISTTQ KPFSSSTPT VLPLSFGTT SSTIGTTVK 
      PTVPGFFVT KNVIPKSEI ARPRPFQLP PEPDFGQPI 
      FGTTQNLVT TLKPTYPTY KISSASPFS GPSTPEPTL 
      PTPFATTQV LPSTFETVD SMITALAEM ANSNNYTEN 
      PRPGLVIPP SAGPQTLHT LAQYFANAL DGIATEKED 
      EEDGDEENL TKVELEKKK LTELLTQMT MNRYNELFP 
      EDESTTTVV PNDLEGQHS KSPATPAPK VRQLAKVFT 
      QALSSYLDD PATFKKVLE EVRPTEPPA LDETTDLPD 
      DELLNFSDA DTKSSYSPF FPPKLPAVR PTWGYLIAY 
      NTTPHNLDV KNSIGGTEE NLQGADSQS FVSQFNKLQ 
      NQKESTTRS SVLLPEDHW TTSPDATKL WKTAFSFDP 
      ASINHNFGT TEPTIGTTT EEVVPAREV RYELHALPK 
      LDLNSTQVH GILIDFMNT TKQDEDNRL QRILRKLNT 
      TEDEFLAKM KEIEANPLT RRLILLLIS ECGNSTQAV 
      PLHTLLIQN STEVLQTSS STHGFKNID PSLDNDSQD 
      ARALQLLNS LYTIASRFG K
    CDS sequence
    • >TC011101 RA
      atggctagg taccacctt ctcaaattg ggattggct 
      atactggtg tattttacg cggtcgatg ggttgtttg 
      gaaactgaa gagaggaaa tttaatgaa gaagactta 
      gttttctat cagggagtt acaggaaaa ccaggtgtt 
      gacttcccg gtactatca catattccg cgtactacc 
      tttaattgc cgaaacgtt gattcgggt tattatgcc 
      gatttggaa acggactgt caagttttc cacatttgt 
      gaagaaggg aagaaaatt tcgttcctg tgccctaac 
      ggtaccatc ttccaacag tcggaactt atctgcgag 
      tggtggttc aaggtcaat tgtaccgat tcgcccaat 
      ttttacgaa gaaagtgcc gaacagtta agggaagat 
      aacgcaaga agaaaagct tcaagaaga gtactacaa 
      aaccatggt gctgttatg cgaactgaa gaacattct 
      tcggtttct gcgagtcag aatggacgc aaatttaac 
      gttggtacc acttttcaa gagaccaat gatttagca 
      agagctcca agttccgac tttgtgagg gatcagaaa 
      aagaagggt agaaaaccg gtgcaattc cagacgcaa 
      tcaattgaa gaacggaag agttttgat gatcaaaag 
      gtggtacca agtaaaaat catcgagcg aacgaacga 
      attaatcaa aatggtgca gtaccacag catcaagaa 
      gttcgaaat aacagtgcc agaagttat aaccagttt 
      ggtactacc agacaagaa gtagtacca aacaatcaa 
      cgtaatgga ggcaggaag gtggttcaa ggatcacaa 
      gaccaaaac cagaagttt gaaacaaat gccaaagga 
      tttggtact tcagtggta ccaaatagg caggagaaa 
      gaacaacat tctaatcct aattttcaa caatatagc 
      aactttcac aaccaaaat agtggattt ggcaccact 
      agcgacttt aagaaaatg gtacctagt caattcaac 
      actcaacaa agtcagcaa gaaaggaaa aatataaaa 
      actttcggt actaaagaa gttgtatca aatcaattt 
      aacaatgga caatcggca caaaatact tttagtaat 
      agtacaaac agagaggag gagattaac ccatttttc 
      aataacggg ttccaagac tcaaacaca aatagtgcc 
      gaaagtacc gacaaaaaa tcgaatgtt tccaacttc 
      tctcaatcg ttcagtcat cgaaatttt aatgtagat 
      gagaaacga acaacgttt agttccagt accaccacc 
      acgccacgg agtaccact aaagtacca cccacgagc 
      tctaacttg caaaaccat aatcaagat taccaccaa 
      attaaaaat ttaaatatt gaagggaag ggtggtacc 
      atcaaacaa acaagtacc gtacccttg acgcggaac 
      gacttagtg aaccgacga ctggaaccc aacgactct 
      gatgagtct caaattacg caagaaacg tcttcgttt 
      tacaagaac tcgttcaat cgaatcgac caaactatc 
      aaaactacc agcaatccg cacccgaac catcgtagt 
      acctatagc gaaatcaag gaacccaaa tttcactca 
      actaaggct tatacatcg ctttcttca gtcacccca 
      caatatttg accactcaa cctataaat aacggacgg 
      ccctttgta ggtagccag gtcggtacc accttgcga 
      gggtttggt accaaacca ccaacaact accccggtg 
      gtaccaaca gaaatattt aatgttggt cgtaccgat 
      aagtcttta aaaccaatc gctttgtct acactgtct 
      acttttaca cctttgcca ccgcttgat ttgacggta 
      ccatcgcaa ggttccttt aacacgggt accccgagc 
      cctaaaact gagagtagt actagggtt atagaccac 
      gctacggtt tatggtaaa tttgttaaa agtggagcc 
      acaactccc acaacagga cgtaccaaa attttgcta 
      tcaaaatca gtttttggt accacccct aagacatca 
      gccgaaatt ttaagtacc tctgatagc tacaatcgg 
      ggtaattcg caagtcaga tttttcggg aaagttcaa 
      accacgact aaaatacca ggcgtccaa attatttcg 
      aaatcggat ggtaccact acaaaaccg gccgaaatt 
      ttgagtacc tctaacagt tacaatcgg ggtaattcg 
      caagttcaa ttttttggc aaagtccaa actacggcc 
      aatttgaac tcaattagt accactcag aaaccattc 
      agtagtagc acaccaact gtactacct ttatcgttt 
      ggcactact agtagtacc attggcacc actgtaaaa 
      ccaacggtt cctgggttt ttcgtgacc aaaaatgtt 
      attccgaag agtgaaata gcccgtcct cgcccattc 
      caactacct ccagaaccc gattttggt caacccatt 
      tttggtacc acccagaat ttggtcaca acactcaaa 
      ccaacgtat cccacatac aaaatttcg tcagcgagt 
      ccattttcg ggaccttca accccagaa cccactctt 
      ccaacccca ttcgccacc actcaggta ctaccttca 
      acttttgaa accgtggac agtatgatc acagctttg 
      gctgaaatg gccaatagt aataactac accgaaaat 
      ccaagaccc ggacttgtg attcctccg tcagccggt 
      ccccaaacc ctccacaca cttgcacag tactttgca 
      aatgccctg gatggaata gcaacggaa aaagaagac 
      gaagaagac ggtgatgaa gaaaacttg accaaagtc 
      gagctcgag aagaaaaaa ctgacagaa ctactgact 
      caaatgaca atgaaccgg tacaacgag ctcttcccc 
      gaggatgaa agtaccacc acggtggta cccaacgac 
      ttggaaggc caacactcc aaaagtcca gcaacccca 
      gcaccaaaa gtgcgtcaa ctggctaaa gttttcacc 
      caagccctt tcgtcctat ttggacgac ccagcgacc 
      ttcaaaaaa gtacttgaa gaggtacga cccacggaa 
      ccaccagca ctggacgaa accaccgat ttaccagac 
      gacgagttg ttgaacttc tcggacgct gacaccaaa 
      tctagttat tcgcccttt ttcccaccc aaattacca 
      gcggtacga cctacatgg gggtacctc attgcctac 
      aacacaacc ccacataat ctagacgtc aagaactca 
      atcggagga acggaagaa aacttgcaa ggagcagac 
      agccagtca tttgtgtcc caattcaac aaattgcaa 
      aaccagaaa gaaagcacc accagaagc tcggtacta 
      cttccagaa gatcactgg acgacgtct cctgacgct 
      accaagctg tggaaaact gcgttttcc tttgatcca 
      gcttcaatc aaccataat tttggcacc actgaacct 
      accattggt actaccacc gaagaagta gtaccagca 
      cgcgaagtt cgctacgaa ttgcacgct ttaccaaaa 
      cttgactta aactcgact caggttcat ggtattttg 
      atcgatttt atgaatacg accaaacag gatgaagat 
      aaccgatta caaaggatt ttgcgtaaa cttaacact 
      accgaagat gagtttttg gcaaaaatg aaggaaatc 
      gaggcaaat ccgttaact cgaaggctg attcttctc 
      ctgattagt gagtgcggt aacagtacc caagcggta 
      ccgctccat accctcctc atccaaaat agtaccgag 
      gtgcttcaa acgtcgagt agtacccat gggttcaag 
      aacattgat ccgagtctg gataatgac agtcaggac 
      gcaagggcg ttgcaattg ttgaattct ttgtatact 
      atagcttcg aggtttggt aaatga
    mRNA sequence
    • >TC011101 RA
      aaaggttaa gatgtcgtg ccatccacg ttcataatt 
      catccgatt gttataatt cagcatccg aatctcgaa 
      tggcggggc tgtgaaaat atttcgaat ggcctttgt 
      tgcgtgtga gtgttgtga aaggtcgta ccgtgatga 
      agaaactag agaggagga gagagagcg cgattgttt 
      gggtccatc tcgacatct tcaccgact gactgcagt 
      cttcagtct tcagcgtac tgtcgtctt caatgcggg 
      ttaaacgtg acggtgcgg gacttaccg gaacgacgg 
      tgtgattat ttatttgag tgtgtttga actattttt 
      ttcgacgga cgtgtgatg gataggagt gcgtccacc 
      atggctagg taccacctt ctcaaattg ggattggct 
      atactggtg tattttacg cggtcgatg ggttgtttg 
      gaaactgaa gagaggaaa tttaatgaa gaagactta 
      gttttctat cagggagtt acaggaaaa ccaggtgtt 
      gacttcccg gtactatca catattccg cgtactacc 
      tttaattgc cgaaacgtt gattcgggt tattatgcc 
      gatttggaa acggactgt caagttttc cacatttgt 
      gaagaaggg aagaaaatt tcgttcctg tgccctaac 
      ggtaccatc ttccaacag tcggaactt atctgcgag 
      tggtggttc aaggtcaat tgtaccgat tcgcccaat 
      ttttacgaa gaaagtgcc gaacagtta agggaagat 
      aacgcaaga agaaaagct tcaagaaga gtactacaa 
      aaccatggt gctgttatg cgaactgaa gaacattct 
      tcggtttct gcgagtcag aatggacgc aaatttaac 
      gttggtacc acttttcaa gagaccaat gatttagca 
      agagctcca agttccgac tttgtgagg gatcagaaa 
      aagaagggt agaaaaccg gtgcaattc cagacgcaa 
      tcaattgaa gaacggaag agttttgat gatcaaaag 
      gtggtacca agtaaaaat catcgagcg aacgaacga 
      attaatcaa aatggtgca gtaccacag catcaagaa 
      gttcgaaat aacagtgcc agaagttat aaccagttt 
      ggtactacc agacaagaa gtagtacca aacaatcaa 
      cgtaatgga ggcaggaag gtggttcaa ggatcacaa 
      gaccaaaac cagaagttt gaaacaaat gccaaagga 
      tttggtact tcagtggta ccaaatagg caggagaaa 
      gaacaacat tctaatcct aattttcaa caatatagc 
      aactttcac aaccaaaat agtggattt ggcaccact 
      agcgacttt aagaaaatg gtacctagt caattcaac 
      actcaacaa agtcagcaa gaaaggaaa aatataaaa 
      actttcggt actaaagaa gttgtatca aatcaattt 
      aacaatgga caatcggca caaaatact tttagtaat 
      agtacaaac agagaggag gagattaac ccatttttc 
      aataacggg ttccaagac tcaaacaca aatagtgcc 
      gaaagtacc gacaaaaaa tcgaatgtt tccaacttc 
      tctcaatcg ttcagtcat cgaaatttt aatgtagat 
      gagaaacga acaacgttt agttccagt accaccacc 
      acgccacgg agtaccact aaagtacca cccacgagc 
      tctaacttg caaaaccat aatcaagat taccaccaa 
      attaaaaat ttaaatatt gaagggaag ggtggtacc 
      atcaaacaa acaagtacc gtacccttg acgcggaac 
      gacttagtg aaccgacga ctggaaccc aacgactct 
      gatgagtct caaattacg caagaaacg tcttcgttt 
      tacaagaac tcgttcaat cgaatcgac caaactatc 
      aaaactacc agcaatccg cacccgaac catcgtagt 
      acctatagc gaaatcaag gaacccaaa tttcactca 
      actaaggct tatacatcg ctttcttca gtcacccca 
      caatatttg accactcaa cctataaat aacggacgg 
      ccctttgta ggtagccag gtcggtacc accttgcga 
      gggtttggt accaaacca ccaacaact accccggtg 
      gtaccaaca gaaatattt aatgttggt cgtaccgat 
      aagtcttta aaaccaatc gctttgtct acactgtct 
      acttttaca cctttgcca ccgcttgat ttgacggta 
      ccatcgcaa ggttccttt aacacgggt accccgagc 
      cctaaaact gagagtagt actagggtt atagaccac 
      gctacggtt tatggtaaa tttgttaaa agtggagcc 
      acaactccc acaacagga cgtaccaaa attttgcta 
      tcaaaatca gtttttggt accacccct aagacatca 
      gccgaaatt ttaagtacc tctgatagc tacaatcgg 
      ggtaattcg caagtcaga tttttcggg aaagttcaa 
      accacgact aaaatacca ggcgtccaa attatttcg 
      aaatcggat ggtaccact acaaaaccg gccgaaatt 
      ttgagtacc tctaacagt tacaatcgg ggtaattcg 
      caagttcaa ttttttggc aaagtccaa actacggcc 
      aatttgaac tcaattagt accactcag aaaccattc 
      agtagtagc acaccaact gtactacct ttatcgttt 
      ggcactact agtagtacc attggcacc actgtaaaa 
      ccaacggtt cctgggttt ttcgtgacc aaaaatgtt 
      attccgaag agtgaaata gcccgtcct cgcccattc 
      caactacct ccagaaccc gattttggt caacccatt 
      tttggtacc acccagaat ttggtcaca acactcaaa 
      ccaacgtat cccacatac aaaatttcg tcagcgagt 
      ccattttcg ggaccttca accccagaa cccactctt 
      ccaacccca ttcgccacc actcaggta ctaccttca 
      acttttgaa accgtggac agtatgatc acagctttg 
      gctgaaatg gccaatagt aataactac accgaaaat 
      ccaagaccc ggacttgtg attcctccg tcagccggt 
      ccccaaacc ctccacaca cttgcacag tactttgca 
      aatgccctg gatggaata gcaacggaa aaagaagac 
      gaagaagac ggtgatgaa gaaaacttg accaaagtc 
      gagctcgag aagaaaaaa ctgacagaa ctactgact 
      caaatgaca atgaaccgg tacaacgag ctcttcccc 
      gaggatgaa agtaccacc acggtggta cccaacgac 
      ttggaaggc caacactcc aaaagtcca gcaacccca 
      gcaccaaaa gtgcgtcaa ctggctaaa gttttcacc 
      caagccctt tcgtcctat ttggacgac ccagcgacc 
      ttcaaaaaa gtacttgaa gaggtacga cccacggaa 
      ccaccagca ctggacgaa accaccgat ttaccagac 
      gacgagttg ttgaacttc tcggacgct gacaccaaa 
      tctagttat tcgcccttt ttcccaccc aaattacca 
      gcggtacga cctacatgg gggtacctc attgcctac 
      aacacaacc ccacataat ctagacgtc aagaactca 
      atcggagga acggaagaa aacttgcaa ggagcagac 
      agccagtca tttgtgtcc caattcaac aaattgcaa 
      aaccagaaa gaaagcacc accagaagc tcggtacta 
      cttccagaa gatcactgg acgacgtct cctgacgct 
      accaagctg tggaaaact gcgttttcc tttgatcca 
      gcttcaatc aaccataat tttggcacc actgaacct 
      accattggt actaccacc gaagaagta gtaccagca 
      cgcgaagtt cgctacgaa ttgcacgct ttaccaaaa 
      cttgactta aactcgact caggttcat ggtattttg 
      atcgatttt atgaatacg accaaacag gatgaagat 
      aaccgatta caaaggatt ttgcgtaaa cttaacact 
      accgaagat gagtttttg gcaaaaatg aaggaaatc 
      gaggcaaat ccgttaact cgaaggctg attcttctc 
      ctgattagt gagtgcggt aacagtacc caagcggta 
      ccgctccat accctcctc atccaaaat agtaccgag 
      gtgcttcaa acgtcgagt agtacccat gggttcaag 
      aacattgat ccgagtctg gataatgac agtcaggac 
      gcaagggcg ttgcaattg ttgaattct ttgtatact 
      atagcttcg aggtttggt aaatgaggt gctcagatc 
      gattagaaa attgagtga atgttgagt gatattagt 
      taagtattt aagattttt tatcatttt gttaattcc 
      tatcagtgt aatcgatta atgtaaatt ttgtaaata 
      aattgttat tttatag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY