Status
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Gene information
General information
TC010461 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7700854..7709795
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010461
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06288

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010461 RA
      MPSVGSAGR SLFLLLAPF FLLPLESSA GLACLSNPC 
      VHGVCLDDL NSTYFCYCI DGYTGIQCQ KNWNECWSS 
      PCKNGGICI DGVASFNCS CPPGFVGEL CEEDFNECE 
      SNPCLNNGT CLDAANGYF CSCLPGYSG VHCEVDVAV 
      CNATNETRC ANGGICVEG PGDSFSCKC QPGWEGLLC 
      EGEVDECMS APCQNGAVC VDLMADYSC ACLFGFSGR 
      NCEDTVKIC DVNPCANGA LCLKEDRQP VCYCVPDFH 
      GDLCQFQYD ECQLGPKCM NGGTCVDGI DNSTCSCPP 
      NLTGVQCEC LILDNGGLD CTYTSPQTV PPIRTSTIF 
      HPTTTETEV PNTTTVQPF STTIAWTTT EVVPPTTTY 
      TQTQSTTEL TETILVYST TPKVTEMSV VTTETEPTT 
      ETTYSTVTT TEIYTEPVF TTTTGKIIT TMLSTETSS 
      FPPVTTFLP MTSTPKQES NFSLPDETT TSFFTKKPE 
      TTTALTAFF TETTTKLLT DTTVVPFDC TKTHCQNGG 
      TCVYKSELG YQCLCPFHT EGDFCELKM GVQKAAFTG 
      QSYLKHRLP DSTNISIEL DAKTLSSKG LLFYSNTDS 
      TYMVLYIEN GHLKFKFSC GYQTMLLSE LKVPVNNGD 
      LMKIKAKLE FSKDLKRCD ASIKVNDSL SMTGDQLAL 
      KRRFSKPSG WLYLGGVPE EIANINLPE SGFVGCMLR 
      LKISQKFLD IFNDAEDGY GVTECSSLA CLSNPCSNG 
      ATCYSHGEK WTCHCKNGF LGKMCEKSV CDTNPCLFG 
      GTCIQFSGS GYICLCPFG KHGHFCEND LKITEPNFA 
      STIRGLSSY VAYPIPGGI SENMEIRFR FLPTVMDQI 
      SLLMFMGQK GHHNYYSDH MAVSFVKGY IMLTWNLGS 
      GPRRIFTPQ PIDEGAKNY QVRVGRSGR RAWLDVENL 
      GNVTGRSPG NLIQLDVSP VLYLGGHDS LNFSTLPHD 
      LPLHTGFSG CIFDVELIT GNLVIPLQG SRQTIGRGV 
      GQCGTTECY EQSCQNGGI CLHHASTFM CLCQDGWFG 
      PLCTFRRNP CDSDYHKCE AGATCVPVI SDYECDCPI 
      GKSGKFCEK DEDITDVNF TGLRSFLSL PPTEFSENH 
      FNIEMEIRP LGDNGLLFF IEKPGSSFI SLTLSSGSL 
      ELRILSYKN KAAPNEPTT IRSTKILIK GIWYKIQLG 
      IFGKKAYLS VDNIINSAV LSTENGINM SKENIFIGG 
      HPDISTLPL LAVSKLPVP YKGCIRAFS ANGVHIPLN 
      KMSILSGRN INDCDGTPC GKDACLNGG TCWLDSFMK 
      PHCSCPPAF YGNKCQSVS ECDTAKCKT TGVCVGPKC 
      SCHLGWEGV YCERQIKVT TPEFTGRSY LIVKKNNEK 
      KRNTPGVEV KTLYLNFTT VKKDGLVLW SRKENSFVG 
      MGLENGFLK IAYSVDNRT GTNLVTLPL SYKISDGLW 
      HNIELSFAP FLIKLDQNK IVYNGKNKK MLENSTMST 
      DGLFYIGGI PTKTTIKKE TNGTFLDTF EGCIEGFAT 
      NGEQVIRDF TPFEGRDVN VCRVV
    CDS sequence
    • >TC010461 RA
      atgccgtca gtcggtagc gccgggaga agcctattc 
      ctcctccta gcccccttt ttccttctc cccctggag 
      tcttcagcc ggcctggcc tgcctcagc aacccctgc 
      gtgcacggc gtctgcttg gacgacctg aattcgact 
      tacttctgc tactgcata gatggctac acgggaatc 
      cagtgccag aaaaactgg aacgagtgt tggtcatcg 
      ccttgcaaa aacgggggc atttgcatt gacggagtt 
      gcctcgttt aactgtagc tgtccgccg ggatttgtc 
      ggcgaatta tgcgaggaa gatttcaac gagtgcgaa 
      agcaacccc tgtttgaac aatggcacg tgtcttgat 
      gccgccaac gggtacttt tgcagttgc ttgcccggg 
      tattcaggg gttcattgc gaggtggat gtggccgtt 
      tgtaacgcc accaacgaa acgaggtgt gcgaatggg 
      gggatttgt gtggaggga cctggagat tccttttct 
      tgtaaatgt cagccaggg tgggaagga cttctctgt 
      gagggtgaa gtggacgag tgtatgtcg gcgccgtgc 
      caaaatggg gctgtttgt gtcgacctg atggccgat 
      tattcgtgc gcttgcctc tttggtttc tcgggacgg 
      aactgtgaa gacaccgtg aaaatttgc gacgtcaat 
      ccttgcgca aacggcgct ctttgcctc aaggaagat 
      cggcaacca gtctgttac tgtgttccg gattttcac 
      ggcgatttg tgtcagttt caatacgac gaatgccaa 
      ctggggccc aaatgcatg aatggtggt acttgtgtt 
      gacggaata gacaactcc acttgttcg tgcccgcca 
      aacctcaca ggtgttcag tgtgagtgc cttatttta 
      gacaacggt ggtttggac tgcacttac acaagtcca 
      caaaccgtt ccacccatt aggacttca accattttt 
      cacccaaca accacagaa acagaagtg ccaaacact 
      actactgtc caaccattt tcaacgact attgcttgg 
      actaccact gaagttgta ccgccaaca acaacatac 
      acacaaacc caaagtacc actgagtta actgagaca 
      atcctcgtc tactcaaca acaccaaaa gtaacagaa 
      atgagtgtg gttaccacc gaaactgaa ccaacaacc 
      gaaaccact tacagtacc gtaaccaca accgaaatt 
      tatacagaa cctgtattt actaccact acgggtaaa 
      attattacc acaatgttg tcaactgaa acttcctct 
      tttcctccc gttaccact ttcctcccc atgacttcc 
      acccccaaa caagaatca aacttttct cttcccgac 
      gaaacaaca acctccttt tttaccaaa aaaccggag 
      acaacaact gctctcacg gcctttttc accgaaaca 
      acaacaaaa ctattgact gacaccacc gttgttccc 
      ttcgactgt acgaaaacc cactgccag aatggcggg 
      acatgtgtt tacaaatcc gagttagga tatcaatgt 
      ttgtgtcct tttcacacc gaaggcgac ttttgcgag 
      cttaaaatg ggcgtgcaa aaggcggct ttcaccggc 
      cagtcgtac ctcaaacac cgacttccc gacagtacg 
      aacatttca atagaatta gatgcaaaa acactgtct 
      tcgaaaggt ttgctgttc tattctaat acggactcg 
      acttatatg gtactatac atcgaaaat ggtcatttg 
      aaatttaaa ttttcgtgt gggtaccaa actatgtta 
      cttagcgaa ctcaaagtg cccgtgaat aatggtgat 
      cttatgaaa atcaaagca aaattggag tttagcaaa 
      gacctgaag cgatgcgat gcttcaatc aaagtcaat 
      gattctctt tcaatgact ggggaccag ctagcactc 
      aaaagacgc ttttccaag ccttccggt tggttgtat 
      ttaggtggt gttccggag gaaattgca aatattaac 
      ttaccagag agtggtttt gtcgggtgt atgctcagg 
      cttaagatt tcgcaaaaa tttttagac attttcaat 
      gacgcggaa gacggttat ggtgtgaca gaatgctct 
      tctttggcc tgtttgtct aacccttgc agtaacgga 
      gctacttgt tactcacac ggggaaaaa tggacgtgt 
      cattgcaaa aatgggttt ttgggcaaa atgtgcgag 
      aaatcggtt tgtgacacc aacccgtgc ctctttggc 
      ggcacttgc atccaattt tcgggcagt ggctacatc 
      tgtttgtgt ccatttggc aaacacggc cacttctgc 
      gaaaacgac ttgaagata actgagccg aactttgcg 
      tcaacaatt cgcggattg tcctcatat gtcgcttac 
      ccaatcccc ggcggaatt tcggagaat atggaaatc 
      aggttcaga tttctccca actgtgatg gaccaaatt 
      tcgctgctg atgttcatg ggtcagaag ggtcaccac 
      aattattat tccgaccac atggcggtg agtttcgtc 
      aagggttac atcatgctg acgtggaat ctgggctcc 
      ggaccccgg aggattttc accccgcag cccatcgac 
      gaaggggcg aaaaactac caagtcagg gtggggcgg 
      tccggacgg cgggcttgg ctggatgtg gagaatttg 
      gggaatgtg actgggagg tccccgggg aatttaatc 
      cagttagat gtgtccccg gttttgtat cttggggga 
      catgattct ttgaatttc tccactctt ccgcatgat 
      ctgcccctg catacggga ttctcaggt tgtattttc 
      gacgtggag ctgatcacc gggaattta gtgattccc 
      ctgcaaggg tccaggcag acaataggg agaggcgtg 
      ggacagtgt ggaacgacg gaatgttac gagcaaagt 
      tgccagaat gggggaatt tgtctccac catgcgagc 
      acgttcatg tgtctgtgc caggatgga tggtttggc 
      cctttgtgc acttttagg agaaacccc tgcgattcg 
      gattatcac aaatgcgag gctggggcc acttgtgtg 
      ccagttatt tcggactat gagtgcgat tgtccgatt 
      ggaaagtcg ggaaaattt tgcgagaaa gatgaggac 
      attactgac gtcaatttc accggcttg agatcattc 
      ctttctctc ccaccaacg gaattcagc gaaaatcac 
      tttaacatt gagatggaa attcgccca ttgggcgac 
      aatggcttg ttatttttt atagagaaa cctggatcc 
      agtttcatt tcattaact ttgtctagt ggaagttta 
      gaattgaga atactctca tacaaaaat aaagctgcc 
      ccgaacgaa cctacgaca atcagaagc acgaaaata 
      ctgatcaaa ggcatttgg tataaaata cagttggga 
      atatttggg aaaaaggcg tacttgtcg gttgataac 
      ataatcaat tctgcagtt ttgagtacg gaaaatgga 
      attaatatg tcaaaagag aacatattt ataggaggt 
      caccccgac atttccacc cttcccttg ctcgcagtg 
      tcaaaactc cccgtccct tacaagggc tgcatccgc 
      gccttttcc gcaaacggg gtgcacatt ccgctcaac 
      aaaatgagc atattaagc ggccgaaac atcaacgat 
      tgtgacggc accccgtgc gggaaagac gcctgttta 
      aatgggggc acttgctgg ctggactca ttcatgaaa 
      ccgcactgc tcctgtccc ccagctttc tacggaaat 
      aaatgccaa tctgtctcg gaatgtgac accgcgaaa 
      tgtaaaacc actggagtg tgtgtcggc cccaagtgt 
      tcgtgtcac ctaggatgg gagggagtt tactgcgaa 
      aggcaaatt aaagtcacg acaccggaa ttcaccggc 
      cggagttac ctcatagtt aagaaaaac aacgaaaaa 
      aagagaaac actcccgga gttgaagtc aagacgttg 
      tatttgaat tttacgacg gtcaagaag gatggactt 
      gtcttgtgg agtagaaaa gaaaattcg ttcgttggt 
      atgggcctt gagaacggg tttttgaaa atcgcatat 
      tcggttgac aacaggact ggaaccaac ttggttact 
      ttgcctttg tcttataaa atttcagac ggtttgtgg 
      cacaacatc gagctcagt tttgcgccc tttttgata 
      aaactggac caaaataaa attgtttac aacggcaag 
      aataaaaaa atgttggag aacagcaca atgtccacc 
      gatgggcta ttctacatt gggggcatt cccacaaaa 
      acgaccatc aagaaggag acaaacggc acttttctc 
      gatactttc gaaggttgc attgaaggt tttgccacc 
      aatggtgaa caagtcatc cgtgatttt accccattt 
      gaaggaaga gatgttaac gtatgccga gtggtttaa
    mRNA sequence
    • >TC010461 RA
      acgaatttg tcaaaactc acttatagt ttttagccc 
      ttcggccaa taatgcagt atgccgtca gtcggtagc 
      gccgggaga agcctattc ctcctccta gcccccttt 
      ttccttctc cccctggag tcttcagcc ggcctggcc 
      tgcctcagc aacccctgc gtgcacggc gtctgcttg 
      gacgacctg aattcgact tacttctgc tactgcata 
      gatggctac acgggaatc cagtgccag aaaaactgg 
      aacgagtgt tggtcatcg ccttgcaaa aacgggggc 
      atttgcatt gacggagtt gcctcgttt aactgtagc 
      tgtccgccg ggatttgtc ggcgaatta tgcgaggaa 
      gatttcaac gagtgcgaa agcaacccc tgtttgaac 
      aatggcacg tgtcttgat gccgccaac gggtacttt 
      tgcagttgc ttgcccggg tattcaggg gttcattgc 
      gaggtggat gtggccgtt tgtaacgcc accaacgaa 
      acgaggtgt gcgaatggg gggatttgt gtggaggga 
      cctggagat tccttttct tgtaaatgt cagccaggg 
      tgggaagga cttctctgt gagggtgaa gtggacgag 
      tgtatgtcg gcgccgtgc caaaatggg gctgtttgt 
      gtcgacctg atggccgat tattcgtgc gcttgcctc 
      tttggtttc tcgggacgg aactgtgaa gacaccgtg 
      aaaatttgc gacgtcaat ccttgcgca aacggcgct 
      ctttgcctc aaggaagat cggcaacca gtctgttac 
      tgtgttccg gattttcac ggcgatttg tgtcagttt 
      caatacgac gaatgccaa ctggggccc aaatgcatg 
      aatggtggt acttgtgtt gacggaata gacaactcc 
      acttgttcg tgcccgcca aacctcaca ggtgttcag 
      tgtgagtgc cttatttta gacaacggt ggtttggac 
      tgcacttac acaagtcca caaaccgtt ccacccatt 
      aggacttca accattttt cacccaaca accacagaa 
      acagaagtg ccaaacact actactgtc caaccattt 
      tcaacgact attgcttgg actaccact gaagttgta 
      ccgccaaca acaacatac acacaaacc caaagtacc 
      actgagtta actgagaca atcctcgtc tactcaaca 
      acaccaaaa gtaacagaa atgagtgtg gttaccacc 
      gaaactgaa ccaacaacc gaaaccact tacagtacc 
      gtaaccaca accgaaatt tatacagaa cctgtattt 
      actaccact acgggtaaa attattacc acaatgttg 
      tcaactgaa acttcctct tttcctccc gttaccact 
      ttcctcccc atgacttcc acccccaaa caagaatca 
      aacttttct cttcccgac gaaacaaca acctccttt 
      tttaccaaa aaaccggag acaacaact gctctcacg 
      gcctttttc accgaaaca acaacaaaa ctattgact 
      gacaccacc gttgttccc ttcgactgt acgaaaacc 
      cactgccag aatggcggg acatgtgtt tacaaatcc 
      gagttagga tatcaatgt ttgtgtcct tttcacacc 
      gaaggcgac ttttgcgag cttaaaatg ggcgtgcaa 
      aaggcggct ttcaccggc cagtcgtac ctcaaacac 
      cgacttccc gacagtacg aacatttca atagaatta 
      gatgcaaaa acactgtct tcgaaaggt ttgctgttc 
      tattctaat acggactcg acttatatg gtactatac 
      atcgaaaat ggtcatttg aaatttaaa ttttcgtgt 
      gggtaccaa actatgtta cttagcgaa ctcaaagtg 
      cccgtgaat aatggtgat cttatgaaa atcaaagca 
      aaattggag tttagcaaa gacctgaag cgatgcgat 
      gcttcaatc aaagtcaat gattctctt tcaatgact 
      ggggaccag ctagcactc aaaagacgc ttttccaag 
      ccttccggt tggttgtat ttaggtggt gttccggag 
      gaaattgca aatattaac ttaccagag agtggtttt 
      gtcgggtgt atgctcagg cttaagatt tcgcaaaaa 
      tttttagac attttcaat gacgcggaa gacggttat 
      ggtgtgaca gaatgctct tctttggcc tgtttgtct 
      aacccttgc agtaacgga gctacttgt tactcacac 
      ggggaaaaa tggacgtgt cattgcaaa aatgggttt 
      ttgggcaaa atgtgcgag aaatcggtt tgtgacacc 
      aacccgtgc ctctttggc ggcacttgc atccaattt 
      tcgggcagt ggctacatc tgtttgtgt ccatttggc 
      aaacacggc cacttctgc gaaaacgac ttgaagata 
      actgagccg aactttgcg tcaacaatt cgcggattg 
      tcctcatat gtcgcttac ccaatcccc ggcggaatt 
      tcggagaat atggaaatc aggttcaga tttctccca 
      actgtgatg gaccaaatt tcgctgctg atgttcatg 
      ggtcagaag ggtcaccac aattattat tccgaccac 
      atggcggtg agtttcgtc aagggttac atcatgctg 
      acgtggaat ctgggctcc ggaccccgg aggattttc 
      accccgcag cccatcgac gaaggggcg aaaaactac 
      caagtcagg gtggggcgg tccggacgg cgggcttgg 
      ctggatgtg gagaatttg gggaatgtg actgggagg 
      tccccgggg aatttaatc cagttagat gtgtccccg 
      gttttgtat cttggggga catgattct ttgaatttc 
      tccactctt ccgcatgat ctgcccctg catacggga 
      ttctcaggt tgtattttc gacgtggag ctgatcacc 
      gggaattta gtgattccc ctgcaaggg tccaggcag 
      acaataggg agaggcgtg ggacagtgt ggaacgacg 
      gaatgttac gagcaaagt tgccagaat gggggaatt 
      tgtctccac catgcgagc acgttcatg tgtctgtgc 
      caggatgga tggtttggc cctttgtgc acttttagg 
      agaaacccc tgcgattcg gattatcac aaatgcgag 
      gctggggcc acttgtgtg ccagttatt tcggactat 
      gagtgcgat tgtccgatt ggaaagtcg ggaaaattt 
      tgcgagaaa gatgaggac attactgac gtcaatttc 
      accggcttg agatcattc ctttctctc ccaccaacg 
      gaattcagc gaaaatcac tttaacatt gagatggaa 
      attcgccca ttgggcgac aatggcttg ttatttttt 
      atagagaaa cctggatcc agtttcatt tcattaact 
      ttgtctagt ggaagttta gaattgaga atactctca 
      tacaaaaat aaagctgcc ccgaacgaa cctacgaca 
      atcagaagc acgaaaata ctgatcaaa ggcatttgg 
      tataaaata cagttggga atatttggg aaaaaggcg 
      tacttgtcg gttgataac ataatcaat tctgcagtt 
      ttgagtacg gaaaatgga attaatatg tcaaaagag 
      aacatattt ataggaggt caccccgac atttccacc 
      cttcccttg ctcgcagtg tcaaaactc cccgtccct 
      tacaagggc tgcatccgc gccttttcc gcaaacggg 
      gtgcacatt ccgctcaac aaaatgagc atattaagc 
      ggccgaaac atcaacgat tgtgacggc accccgtgc 
      gggaaagac gcctgttta aatgggggc acttgctgg 
      ctggactca ttcatgaaa ccgcactgc tcctgtccc 
      ccagctttc tacggaaat aaatgccaa tctgtctcg 
      gaatgtgac accgcgaaa tgtaaaacc actggagtg 
      tgtgtcggc cccaagtgt tcgtgtcac ctaggatgg 
      gagggagtt tactgcgaa aggcaaatt aaagtcacg 
      acaccggaa ttcaccggc cggagttac ctcatagtt 
      aagaaaaac aacgaaaaa aagagaaac actcccgga 
      gttgaagtc aagacgttg tatttgaat tttacgacg 
      gtcaagaag gatggactt gtcttgtgg agtagaaaa 
      gaaaattcg ttcgttggt atgggcctt gagaacggg 
      tttttgaaa atcgcatat tcggttgac aacaggact 
      ggaaccaac ttggttact ttgcctttg tcttataaa 
      atttcagac ggtttgtgg cacaacatc gagctcagt 
      tttgcgccc tttttgata aaactggac caaaataaa 
      attgtttac aacggcaag aataaaaaa atgttggag 
      aacagcaca atgtccacc gatgggcta ttctacatt 
      gggggcatt cccacaaaa acgaccatc aagaaggag 
      acaaacggc acttttctc gatactttc gaaggttgc 
      attgaaggt tttgccacc aatggtgaa caagtcatc 
      cgtgatttt accccattt gaaggaaga gatgttaac 
      gtatgccga gtggtttaa ataattttc cacgcaggg 
      ctgggagaa acctcatca gagagtaga gaatgtttg 
      tagatattc tagatgtct tcgtttttg ttagtagaa 
      gaacagaga aggaggaaa acgcgggat gcagtttta 
      agtgtttat ctttcgtca cgtaatcac tgccaaaac 
      caaacgcta tagataaac actcattta taaatagga 
      cgcacatgc catttgaat gattttgta agaaaacaa 
      tagaagtgt g
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