| General information | |||
| TC010461 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7700854..7709795 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010461 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06288 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010461 RA
MPSVGSAGR SLFLLLAPF FLLPLESSA GLACLSNPC VHGVCLDDL NSTYFCYCI DGYTGIQCQ KNWNECWSS PCKNGGICI DGVASFNCS CPPGFVGEL CEEDFNECE SNPCLNNGT CLDAANGYF CSCLPGYSG VHCEVDVAV CNATNETRC ANGGICVEG PGDSFSCKC QPGWEGLLC EGEVDECMS APCQNGAVC VDLMADYSC ACLFGFSGR NCEDTVKIC DVNPCANGA LCLKEDRQP VCYCVPDFH GDLCQFQYD ECQLGPKCM NGGTCVDGI DNSTCSCPP NLTGVQCEC LILDNGGLD CTYTSPQTV PPIRTSTIF HPTTTETEV PNTTTVQPF STTIAWTTT EVVPPTTTY TQTQSTTEL TETILVYST TPKVTEMSV VTTETEPTT ETTYSTVTT TEIYTEPVF TTTTGKIIT TMLSTETSS FPPVTTFLP MTSTPKQES NFSLPDETT TSFFTKKPE TTTALTAFF TETTTKLLT DTTVVPFDC TKTHCQNGG TCVYKSELG YQCLCPFHT EGDFCELKM GVQKAAFTG QSYLKHRLP DSTNISIEL DAKTLSSKG LLFYSNTDS TYMVLYIEN GHLKFKFSC GYQTMLLSE LKVPVNNGD LMKIKAKLE FSKDLKRCD ASIKVNDSL SMTGDQLAL KRRFSKPSG WLYLGGVPE EIANINLPE SGFVGCMLR LKISQKFLD IFNDAEDGY GVTECSSLA CLSNPCSNG ATCYSHGEK WTCHCKNGF LGKMCEKSV CDTNPCLFG GTCIQFSGS GYICLCPFG KHGHFCEND LKITEPNFA STIRGLSSY VAYPIPGGI SENMEIRFR FLPTVMDQI SLLMFMGQK GHHNYYSDH MAVSFVKGY IMLTWNLGS GPRRIFTPQ PIDEGAKNY QVRVGRSGR RAWLDVENL GNVTGRSPG NLIQLDVSP VLYLGGHDS LNFSTLPHD LPLHTGFSG CIFDVELIT GNLVIPLQG SRQTIGRGV GQCGTTECY EQSCQNGGI CLHHASTFM CLCQDGWFG PLCTFRRNP CDSDYHKCE AGATCVPVI SDYECDCPI GKSGKFCEK DEDITDVNF TGLRSFLSL PPTEFSENH FNIEMEIRP LGDNGLLFF IEKPGSSFI SLTLSSGSL ELRILSYKN KAAPNEPTT IRSTKILIK GIWYKIQLG IFGKKAYLS VDNIINSAV LSTENGINM SKENIFIGG HPDISTLPL LAVSKLPVP YKGCIRAFS ANGVHIPLN KMSILSGRN INDCDGTPC GKDACLNGG TCWLDSFMK PHCSCPPAF YGNKCQSVS ECDTAKCKT TGVCVGPKC SCHLGWEGV YCERQIKVT TPEFTGRSY LIVKKNNEK KRNTPGVEV KTLYLNFTT VKKDGLVLW SRKENSFVG MGLENGFLK IAYSVDNRT GTNLVTLPL SYKISDGLW HNIELSFAP FLIKLDQNK IVYNGKNKK MLENSTMST DGLFYIGGI PTKTTIKKE TNGTFLDTF EGCIEGFAT NGEQVIRDF TPFEGRDVN VCRVV
- >TC010461 RA
atgccgtca gtcggtagc gccgggaga agcctattc ctcctccta gcccccttt ttccttctc cccctggag tcttcagcc ggcctggcc tgcctcagc aacccctgc gtgcacggc gtctgcttg gacgacctg aattcgact tacttctgc tactgcata gatggctac acgggaatc cagtgccag aaaaactgg aacgagtgt tggtcatcg ccttgcaaa aacgggggc atttgcatt gacggagtt gcctcgttt aactgtagc tgtccgccg ggatttgtc ggcgaatta tgcgaggaa gatttcaac gagtgcgaa agcaacccc tgtttgaac aatggcacg tgtcttgat gccgccaac gggtacttt tgcagttgc ttgcccggg tattcaggg gttcattgc gaggtggat gtggccgtt tgtaacgcc accaacgaa acgaggtgt gcgaatggg gggatttgt gtggaggga cctggagat tccttttct tgtaaatgt cagccaggg tgggaagga cttctctgt gagggtgaa gtggacgag tgtatgtcg gcgccgtgc caaaatggg gctgtttgt gtcgacctg atggccgat tattcgtgc gcttgcctc tttggtttc tcgggacgg aactgtgaa gacaccgtg aaaatttgc gacgtcaat ccttgcgca aacggcgct ctttgcctc aaggaagat cggcaacca gtctgttac tgtgttccg gattttcac ggcgatttg tgtcagttt caatacgac gaatgccaa ctggggccc aaatgcatg aatggtggt acttgtgtt gacggaata gacaactcc acttgttcg tgcccgcca aacctcaca ggtgttcag tgtgagtgc cttatttta gacaacggt ggtttggac tgcacttac acaagtcca caaaccgtt ccacccatt aggacttca accattttt cacccaaca accacagaa acagaagtg ccaaacact actactgtc caaccattt tcaacgact attgcttgg actaccact gaagttgta ccgccaaca acaacatac acacaaacc caaagtacc actgagtta actgagaca atcctcgtc tactcaaca acaccaaaa gtaacagaa atgagtgtg gttaccacc gaaactgaa ccaacaacc gaaaccact tacagtacc gtaaccaca accgaaatt tatacagaa cctgtattt actaccact acgggtaaa attattacc acaatgttg tcaactgaa acttcctct tttcctccc gttaccact ttcctcccc atgacttcc acccccaaa caagaatca aacttttct cttcccgac gaaacaaca acctccttt tttaccaaa aaaccggag acaacaact gctctcacg gcctttttc accgaaaca acaacaaaa ctattgact gacaccacc gttgttccc ttcgactgt acgaaaacc cactgccag aatggcggg acatgtgtt tacaaatcc gagttagga tatcaatgt ttgtgtcct tttcacacc gaaggcgac ttttgcgag cttaaaatg ggcgtgcaa aaggcggct ttcaccggc cagtcgtac ctcaaacac cgacttccc gacagtacg aacatttca atagaatta gatgcaaaa acactgtct tcgaaaggt ttgctgttc tattctaat acggactcg acttatatg gtactatac atcgaaaat ggtcatttg aaatttaaa ttttcgtgt gggtaccaa actatgtta cttagcgaa ctcaaagtg cccgtgaat aatggtgat cttatgaaa atcaaagca aaattggag tttagcaaa gacctgaag cgatgcgat gcttcaatc aaagtcaat gattctctt tcaatgact ggggaccag ctagcactc aaaagacgc ttttccaag ccttccggt tggttgtat ttaggtggt gttccggag gaaattgca aatattaac ttaccagag agtggtttt gtcgggtgt atgctcagg cttaagatt tcgcaaaaa tttttagac attttcaat gacgcggaa gacggttat ggtgtgaca gaatgctct tctttggcc tgtttgtct aacccttgc agtaacgga gctacttgt tactcacac ggggaaaaa tggacgtgt cattgcaaa aatgggttt ttgggcaaa atgtgcgag aaatcggtt tgtgacacc aacccgtgc ctctttggc ggcacttgc atccaattt tcgggcagt ggctacatc tgtttgtgt ccatttggc aaacacggc cacttctgc gaaaacgac ttgaagata actgagccg aactttgcg tcaacaatt cgcggattg tcctcatat gtcgcttac ccaatcccc ggcggaatt tcggagaat atggaaatc aggttcaga tttctccca actgtgatg gaccaaatt tcgctgctg atgttcatg ggtcagaag ggtcaccac aattattat tccgaccac atggcggtg agtttcgtc aagggttac atcatgctg acgtggaat ctgggctcc ggaccccgg aggattttc accccgcag cccatcgac gaaggggcg aaaaactac caagtcagg gtggggcgg tccggacgg cgggcttgg ctggatgtg gagaatttg gggaatgtg actgggagg tccccgggg aatttaatc cagttagat gtgtccccg gttttgtat cttggggga catgattct ttgaatttc tccactctt ccgcatgat ctgcccctg catacggga ttctcaggt tgtattttc gacgtggag ctgatcacc gggaattta gtgattccc ctgcaaggg tccaggcag acaataggg agaggcgtg ggacagtgt ggaacgacg gaatgttac gagcaaagt tgccagaat gggggaatt tgtctccac catgcgagc acgttcatg tgtctgtgc caggatgga tggtttggc cctttgtgc acttttagg agaaacccc tgcgattcg gattatcac aaatgcgag gctggggcc acttgtgtg ccagttatt tcggactat gagtgcgat tgtccgatt ggaaagtcg ggaaaattt tgcgagaaa gatgaggac attactgac gtcaatttc accggcttg agatcattc ctttctctc ccaccaacg gaattcagc gaaaatcac tttaacatt gagatggaa attcgccca ttgggcgac aatggcttg ttatttttt atagagaaa cctggatcc agtttcatt tcattaact ttgtctagt ggaagttta gaattgaga atactctca tacaaaaat aaagctgcc ccgaacgaa cctacgaca atcagaagc acgaaaata ctgatcaaa ggcatttgg tataaaata cagttggga atatttggg aaaaaggcg tacttgtcg gttgataac ataatcaat tctgcagtt ttgagtacg gaaaatgga attaatatg tcaaaagag aacatattt ataggaggt caccccgac atttccacc cttcccttg ctcgcagtg tcaaaactc cccgtccct tacaagggc tgcatccgc gccttttcc gcaaacggg gtgcacatt ccgctcaac aaaatgagc atattaagc ggccgaaac atcaacgat tgtgacggc accccgtgc gggaaagac gcctgttta aatgggggc acttgctgg ctggactca ttcatgaaa ccgcactgc tcctgtccc ccagctttc tacggaaat aaatgccaa tctgtctcg gaatgtgac accgcgaaa tgtaaaacc actggagtg tgtgtcggc cccaagtgt tcgtgtcac ctaggatgg gagggagtt tactgcgaa aggcaaatt aaagtcacg acaccggaa ttcaccggc cggagttac ctcatagtt aagaaaaac aacgaaaaa aagagaaac actcccgga gttgaagtc aagacgttg tatttgaat tttacgacg gtcaagaag gatggactt gtcttgtgg agtagaaaa gaaaattcg ttcgttggt atgggcctt gagaacggg tttttgaaa atcgcatat tcggttgac aacaggact ggaaccaac ttggttact ttgcctttg tcttataaa atttcagac ggtttgtgg cacaacatc gagctcagt tttgcgccc tttttgata aaactggac caaaataaa attgtttac aacggcaag aataaaaaa atgttggag aacagcaca atgtccacc gatgggcta ttctacatt gggggcatt cccacaaaa acgaccatc aagaaggag acaaacggc acttttctc gatactttc gaaggttgc attgaaggt tttgccacc aatggtgaa caagtcatc cgtgatttt accccattt gaaggaaga gatgttaac gtatgccga gtggtttaa
- >TC010461 RA
acgaatttg tcaaaactc acttatagt ttttagccc ttcggccaa taatgcagt atgccgtca gtcggtagc gccgggaga agcctattc ctcctccta gcccccttt ttccttctc cccctggag tcttcagcc ggcctggcc tgcctcagc aacccctgc gtgcacggc gtctgcttg gacgacctg aattcgact tacttctgc tactgcata gatggctac acgggaatc cagtgccag aaaaactgg aacgagtgt tggtcatcg ccttgcaaa aacgggggc atttgcatt gacggagtt gcctcgttt aactgtagc tgtccgccg ggatttgtc ggcgaatta tgcgaggaa gatttcaac gagtgcgaa agcaacccc tgtttgaac aatggcacg tgtcttgat gccgccaac gggtacttt tgcagttgc ttgcccggg tattcaggg gttcattgc gaggtggat gtggccgtt tgtaacgcc accaacgaa acgaggtgt gcgaatggg gggatttgt gtggaggga cctggagat tccttttct tgtaaatgt cagccaggg tgggaagga cttctctgt gagggtgaa gtggacgag tgtatgtcg gcgccgtgc caaaatggg gctgtttgt gtcgacctg atggccgat tattcgtgc gcttgcctc tttggtttc tcgggacgg aactgtgaa gacaccgtg aaaatttgc gacgtcaat ccttgcgca aacggcgct ctttgcctc aaggaagat cggcaacca gtctgttac tgtgttccg gattttcac ggcgatttg tgtcagttt caatacgac gaatgccaa ctggggccc aaatgcatg aatggtggt acttgtgtt gacggaata gacaactcc acttgttcg tgcccgcca aacctcaca ggtgttcag tgtgagtgc cttatttta gacaacggt ggtttggac tgcacttac acaagtcca caaaccgtt ccacccatt aggacttca accattttt cacccaaca accacagaa acagaagtg ccaaacact actactgtc caaccattt tcaacgact attgcttgg actaccact gaagttgta ccgccaaca acaacatac acacaaacc caaagtacc actgagtta actgagaca atcctcgtc tactcaaca acaccaaaa gtaacagaa atgagtgtg gttaccacc gaaactgaa ccaacaacc gaaaccact tacagtacc gtaaccaca accgaaatt tatacagaa cctgtattt actaccact acgggtaaa attattacc acaatgttg tcaactgaa acttcctct tttcctccc gttaccact ttcctcccc atgacttcc acccccaaa caagaatca aacttttct cttcccgac gaaacaaca acctccttt tttaccaaa aaaccggag acaacaact gctctcacg gcctttttc accgaaaca acaacaaaa ctattgact gacaccacc gttgttccc ttcgactgt acgaaaacc cactgccag aatggcggg acatgtgtt tacaaatcc gagttagga tatcaatgt ttgtgtcct tttcacacc gaaggcgac ttttgcgag cttaaaatg ggcgtgcaa aaggcggct ttcaccggc cagtcgtac ctcaaacac cgacttccc gacagtacg aacatttca atagaatta gatgcaaaa acactgtct tcgaaaggt ttgctgttc tattctaat acggactcg acttatatg gtactatac atcgaaaat ggtcatttg aaatttaaa ttttcgtgt gggtaccaa actatgtta cttagcgaa ctcaaagtg cccgtgaat aatggtgat cttatgaaa atcaaagca aaattggag tttagcaaa gacctgaag cgatgcgat gcttcaatc aaagtcaat gattctctt tcaatgact ggggaccag ctagcactc aaaagacgc ttttccaag ccttccggt tggttgtat ttaggtggt gttccggag gaaattgca aatattaac ttaccagag agtggtttt gtcgggtgt atgctcagg cttaagatt tcgcaaaaa tttttagac attttcaat gacgcggaa gacggttat ggtgtgaca gaatgctct tctttggcc tgtttgtct aacccttgc agtaacgga gctacttgt tactcacac ggggaaaaa tggacgtgt cattgcaaa aatgggttt ttgggcaaa atgtgcgag aaatcggtt tgtgacacc aacccgtgc ctctttggc ggcacttgc atccaattt tcgggcagt ggctacatc tgtttgtgt ccatttggc aaacacggc cacttctgc gaaaacgac ttgaagata actgagccg aactttgcg tcaacaatt cgcggattg tcctcatat gtcgcttac ccaatcccc ggcggaatt tcggagaat atggaaatc aggttcaga tttctccca actgtgatg gaccaaatt tcgctgctg atgttcatg ggtcagaag ggtcaccac aattattat tccgaccac atggcggtg agtttcgtc aagggttac atcatgctg acgtggaat ctgggctcc ggaccccgg aggattttc accccgcag cccatcgac gaaggggcg aaaaactac caagtcagg gtggggcgg tccggacgg cgggcttgg ctggatgtg gagaatttg gggaatgtg actgggagg tccccgggg aatttaatc cagttagat gtgtccccg gttttgtat cttggggga catgattct ttgaatttc tccactctt ccgcatgat ctgcccctg catacggga ttctcaggt tgtattttc gacgtggag ctgatcacc gggaattta gtgattccc ctgcaaggg tccaggcag acaataggg agaggcgtg ggacagtgt ggaacgacg gaatgttac gagcaaagt tgccagaat gggggaatt tgtctccac catgcgagc acgttcatg tgtctgtgc caggatgga tggtttggc cctttgtgc acttttagg agaaacccc tgcgattcg gattatcac aaatgcgag gctggggcc acttgtgtg ccagttatt tcggactat gagtgcgat tgtccgatt ggaaagtcg ggaaaattt tgcgagaaa gatgaggac attactgac gtcaatttc accggcttg agatcattc ctttctctc ccaccaacg gaattcagc gaaaatcac tttaacatt gagatggaa attcgccca ttgggcgac aatggcttg ttatttttt atagagaaa cctggatcc agtttcatt tcattaact ttgtctagt ggaagttta gaattgaga atactctca tacaaaaat aaagctgcc ccgaacgaa cctacgaca atcagaagc acgaaaata ctgatcaaa ggcatttgg tataaaata cagttggga atatttggg aaaaaggcg tacttgtcg gttgataac ataatcaat tctgcagtt ttgagtacg gaaaatgga attaatatg tcaaaagag aacatattt ataggaggt caccccgac atttccacc cttcccttg ctcgcagtg tcaaaactc cccgtccct tacaagggc tgcatccgc gccttttcc gcaaacggg gtgcacatt ccgctcaac aaaatgagc atattaagc ggccgaaac atcaacgat tgtgacggc accccgtgc gggaaagac gcctgttta aatgggggc acttgctgg ctggactca ttcatgaaa ccgcactgc tcctgtccc ccagctttc tacggaaat aaatgccaa tctgtctcg gaatgtgac accgcgaaa tgtaaaacc actggagtg tgtgtcggc cccaagtgt tcgtgtcac ctaggatgg gagggagtt tactgcgaa aggcaaatt aaagtcacg acaccggaa ttcaccggc cggagttac ctcatagtt aagaaaaac aacgaaaaa aagagaaac actcccgga gttgaagtc aagacgttg tatttgaat tttacgacg gtcaagaag gatggactt gtcttgtgg agtagaaaa gaaaattcg ttcgttggt atgggcctt gagaacggg tttttgaaa atcgcatat tcggttgac aacaggact ggaaccaac ttggttact ttgcctttg tcttataaa atttcagac ggtttgtgg cacaacatc gagctcagt tttgcgccc tttttgata aaactggac caaaataaa attgtttac aacggcaag aataaaaaa atgttggag aacagcaca atgtccacc gatgggcta ttctacatt gggggcatt cccacaaaa acgaccatc aagaaggag acaaacggc acttttctc gatactttc gaaggttgc attgaaggt tttgccacc aatggtgaa caagtcatc cgtgatttt accccattt gaaggaaga gatgttaac gtatgccga gtggtttaa ataattttc cacgcaggg ctgggagaa acctcatca gagagtaga gaatgtttg tagatattc tagatgtct tcgtttttg ttagtagaa gaacagaga aggaggaaa acgcgggat gcagtttta agtgtttat ctttcgtca cgtaatcac tgccaaaac caaacgcta tagataaac actcattta taaatagga cgcacatgc catttgaat gattttgta agaaaacaa tagaagtgt g
