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Gene information
General information
TC010103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15126366..15133435
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010103
    iBeetle screen iB_06284

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010103 RA
      MCDDDDWDT PTSSAPAFS EQRGGVISE GGCKFSSGR 
      GFVPAERND GPSFDSWGD SSNDNGYTN GRKRDDGGN 
      EWGDSGGGG GFSWENNDD SGGGRGRGG RGGRGRGGG 
      RGGFDRGGR GGGRGGFRN DFENGDRGE RGGRGGGRG 
      GGRDGDRGD GGGEEKKRE FYIPPEVEN VEDLFTSGI 
      TTGVNFMKL DEIEVKVTG NDAPPPITS FETSGLRPH 
      LLENVKKSG YTKPTAIQK YAIPVILSG RDLMSCAQT 
      GSGKTAAFM LPIIHNLLS DKNPPNTEN NCAQPVVVI 
      MSPTRELAI QIADQGKKF AYNSTVKVA VIYGGTSTN 
      HQRGRILGG CHILVATPG RLKDFVNRG NVSFNSLKY 
      FVLDEADRM LDMGFLGDV EEMLSHQSM PATGERQTL 
      MFSATFPEE VQQLAGKFL LNYIFIAVG IVGSACTDV 
      EQKFFQVSK FDKRSKLVS ILEKAPNER TLIFVETKR 
      NADFLATFL SEQNIQSTS IHGDRYQSE REKALLDFK 
      TGHRKVLVA TGVAARGLD IKDVQHVIN YDLPKSIDE 
      YVHRIGRTG RVGNKGKAT SFFDEDQDR NLASDLAKI 
      LSQAKQEIP EWLGSKSYG GSADQFGGR DIRGDDFGR 
      GQDFSNAAV QEEEVW
    CDS sequence
    • >TC010103 RA
      atgtgcgac gacgatgat tgggatacg ccgacgagt 
      tcagcccct gcgttttct gagcagagg ggtggcgtg 
      atctccgaa gggggttgc aagttctct tcaggtcgt 
      ggctttgtt ccagctgag cgaaacgac ggcccctcg 
      ttcgattca tggggggac agcagtaac gataacggc 
      tacacaaac gggcgaaag cgagatgat ggtggtaac 
      gagtggggt gattccggg ggtggcggt ggattttct 
      tgggaaaac aacgacgat tcgggcggg ggcagaggc 
      cgagggggc cgcggcggc cgcgggaga gggggtggc 
      cgtggcggg tttgaccgt ggaggccgc ggcggaggt 
      cgagggggc ttccgcaat gatttcgaa aatggggat 
      aggggcgaa cgtggaggt cggggcgga ggccgagga 
      ggggggcgc gatggcgat cgtggagat ggcgggggt 
      gaagagaag aagagggaa ttttatatt ccacctgaa 
      gtcgaaaat gttgaggac ttgtttacc agtggaata 
      acaacagga gtcaatttt atgaagttg gacgaaatt 
      gaagtcaag gtgactggt aatgatgct ccaccaccg 
      atcacttct ttcgaaact tcgggttta agacctcac 
      ttgttagaa aatgtgaaa aaatctgga tatacgaag 
      ccaacggcg attcagaaa tatgcaatt cctgtcatc 
      ttgagcggt cgggatctt atgagttgt gcacaaact 
      ggatctggg aaaactgct gcttttatg ttaccaatt 
      attcataat ctcttaagt gataagaat cctcctaac 
      acagaaaac aattgtgca cagcctgta gttgtgata 
      atgtcgcct actagagaa ttggcaata cagatcgcc 
      gaccaaggc aaaaaattc gcttacaat tcgactgtg 
      aaagttgct gttatttat ggaggaact tcgacaaat 
      catcaacgt ggccgcatt ctgggtggt tgtcacatt 
      ttggttgcg acacctggc cgacttaag gattttgta 
      aatcgtgga aacgtatca ttcaattct ctcaagtat 
      tttgtcctt gatgaagcc gatcggatg ttagacatg 
      ggtttcctt ggtgatgtt gaagagatg ctaagccat 
      cagtcaatg cctgcgact ggcgaacgt caaactttg 
      atgttttcc gcaacattt cccgaagaa gttcaacaa 
      ctcgccggg aaatttctt ctcaactac atctttatt 
      gccgtggga atcgtcggc agtgcatgc accgatgtc 
      gaacagaaa ttcttccaa gtcagtaaa ttcgacaag 
      aggtcgaaa ctcgtttcg attttggaa aaagcgcca 
      aacgaacgt acgctcatt tttgtggag acgaagcga 
      aacgctgat ttcttggcc actttcctc agcgagcaa 
      aatattcaa tctactagc attcatggc gatcgatac 
      caaagcgag agagaaaag gcattgcta gatttcaag 
      actggccat agaaaagtt cttgtcgct actggagtt 
      gcggctaga ggtctcgat atcaaagac gtccagcat 
      gttattaac tacgatttg cctaaatcc attgatgaa 
      tacgtgcat agaattgga agaactggt cgtgttggg 
      aataagggc aaagctacg agctttttt gatgaagac 
      caggatcgg aatctcgcg tcagatctt gcgaaaatc 
      ctctcgcaa gctaagcaa gaaataccg gaatggttg 
      ggtagtaaa agttacggc ggttctgcc gaccagttt 
      ggtggcaga gatattcga ggtgatgat tttgggcgt 
      ggacaagat ttttccaat gccgctgtt caagaagaa 
      gaggtgtgg taa
    mRNA sequence
    • >TC010103 RA
      tagcgagtc catgcttgt tgaaaaact tgcagttgc 
      aagagtgtc aagatactt aaatttctt tcgaccttt 
      atttcgatc acagttcag tttgttcga aaaatgtgc 
      gacgacgat gattgggat acgccgacg agttcagcc 
      cctgcgttt tctgagcag aggggtggc gtgatctcc 
      gaagggggt tgcaagttc tcttcaggt cgtggcttt 
      gttccagct gagcgaaac gacggcccc tcgttcgat 
      tcatggggg gacagcagt aacgataac ggctacaca 
      aacgggcga aagcgagat gatggtggt aacgagtgg 
      ggtgattcc gggggtggc ggtggattt tcttgggaa 
      aacaacgac gattcgggc gggggcaga ggccgaggg 
      ggccgcggc ggccgcggg agagggggt ggccgtggc 
      gggtttgac cgtggaggc cgcggcgga ggtcgaggg 
      ggcttccgc aatgatttc gaaaatggg gataggggc 
      gaacgtgga ggtcggggc ggaggccga ggagggggg 
      cgcgatggc gatcgtgga gatggcggg ggtgaagag 
      aagaagagg gaattttat attccacct gaagtcgaa 
      aatgttgag gacttgttt accagtgga ataacaaca 
      ggagtcaat tttatgaag ttggacgaa attgaagtc 
      aaggtgact ggtaatgat gctccacca ccgatcact 
      tctttcgaa acttcgggt ttaagacct cacttgtta 
      gaaaatgtg aaaaaatct ggatatacg aagccaacg 
      gcgattcag aaatatgca attcctgtc atcttgagc 
      ggtcgggat cttatgagt tgtgcacaa actggatct 
      gggaaaact gctgctttt atgttacca attattcat 
      aatctctta agtgataag aatcctcct aacacagaa 
      aacaattgt gcacagcct gtagttgtg ataatgtcg 
      cctactaga gaattggca atacagatc gccgaccaa 
      ggcaaaaaa ttcgcttac aattcgact gtgaaagtt 
      gctgttatt tatggagga acttcgaca aatcatcaa 
      cgtggccgc attctgggt ggttgtcac attttggtt 
      gcgacacct ggccgactt aaggatttt gtaaatcgt 
      ggaaacgta tcattcaat tctctcaag tattttgtc 
      cttgatgaa gccgatcgg atgttagac atgggtttc 
      cttggtgat gttgaagag atgctaagc catcagtca 
      atgcctgcg actggcgaa cgtcaaact ttgatgttt 
      tccgcaaca tttcccgaa gaagttcaa caactcgcc 
      gggaaattt cttctcaac tacatcttt attgccgtg 
      ggaatcgtc ggcagtgca tgcaccgat gtcgaacag 
      aaattcttc caagtcagt aaattcgac aagaggtcg 
      aaactcgtt tcgattttg gaaaaagcg ccaaacgaa 
      cgtacgctc atttttgtg gagacgaag cgaaacgct 
      gatttcttg gccactttc ctcagcgag caaaatatt 
      caatctact agcattcat ggcgatcga taccaaagc 
      gagagagaa aaggcattg ctagatttc aagactggc 
      catagaaaa gttcttgtc gctactgga gttgcggct 
      agaggtctc gatatcaaa gacgtccag catgttatt 
      aactacgat ttgcctaaa tccattgat gaatacgtg 
      catagaatt ggaagaact ggtcgtgtt gggaataag 
      ggcaaagct acgagcttt tttgatgaa gaccaggat 
      cggaatctc gcgtcagat cttgcgaaa atcctctcg 
      caagctaag caagaaata ccggaatgg ttgggtagt 
      aaaagttac ggcggttct gccgaccag tttggtggc 
      agagatatt cgaggtgat gattttggg cgtggacaa 
      gatttttcc aatgccgct gttcaagaa gaagaggtg 
      tggtaaggc attacacac gttttgtcc agtttttca 
      cattgcgtg acatttaag ttttgttat gttcctagg 
      ttagtttaa aacgtttat tgaagacaa gtttctcta 
      tgaattata gtatttttg ttttgtttt tgaatgttc 
      agttaaatt tttagttct aagtacgta ttcagtttt 
      agtagtatt ttaagtttt gttattcta ccaacgtta 
      agctgtgat acttgactt atgggatta ttagtcatt 
      agtatcctc ttgttttgt agcttttaa tagcattcc 
      aaattacgt caataaata ttttttaca att
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