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Gene information
General information
TC009993 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10606194..10608642
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009993
    iBeetle screen iB_06281

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009993 RA
      MMEIHLPIK NSWKTVNNL KENLKKEAQ ERKRNILHL 
      IFKYLVDNN LLSVAEVLQ NETQTGIHY QVCDNIDLE 
      IVLQEYQSY YYTKFQKQP KILRKTAEN EQVAPLKKE 
      RKSTAKEKQ TLKQEKNDD TFQFEIVTL SKDSSLSDI 
      QSKPLIDFE NYSSEWREM ADQIIKQIV PKTLGITFK 
      DCISLSSTI ETLKEAIIY PLSYPQLFE KACTWRGVL 
      LYGPPGTGK TLLARALAC ESSSTLINV TSSAFISKW 
      RGESEKMVK VLFDVAKFY SPTTIFIDE IDALASTSH 
      DSNHEASRR FKSELLTQI DGIMKTEVF VLATTNNPW 
      SIDNALLRR FEKRILVPL PDKDSRSEL FQYYFSKNG 
      YDFRNEDLA VFVNQTENF SGSDVKNVC KEAEMIVIR 
      EKLEQIRRG DKKKCTRQV KSGDVLNAL KKIKPCIEQ 
      NDYKKYLDW GRKYGAT
    CDS sequence
    • >TC009993 RA
      atgatggaa atccatcta cctatcaaa aacagttgg 
      aaaaccgtc aataacttg aaagaaaac ttgaaaaaa 
      gaggcccaa gagcgcaag cgaaacatt ctccaccta 
      atcttcaaa tacctcgtc gacaacaac ttactctca 
      gtcgccgaa gtgcttcaa aacgaaacc caaaccgga 
      atccactac caagtctgc gacaacatc gacttggag 
      atagtcctc caagaatac caaagttac tattacaca 
      aaattccaa aaacaacca aaaatctta cgaaaaact 
      gccgaaaac gaacaagtg gccccttta aaaaaagag 
      cgaaaatca acagccaaa gaaaaacaa accctaaag 
      caagaaaaa aatgacgac actttccaa ttcgaaatt 
      gtcacatta tcaaaagac tcgagcttg agcgacatc 
      cagtcaaaa ccgctgatc gattttgaa aactactca 
      tcagaatgg cgcgaaatg gccgaccaa ataatcaaa 
      caaatcgta ccgaaaaca ctggggata actttcaag 
      gattgtatc agcctcagc tccactatt gagacgctg 
      aaagaggcg ataatttac cccttgagc tacccccag 
      ctgtttgaa aaagcctgc acttggcga ggcgtcctc 
      ctctacggc cctccaggc accggcaaa accttacta 
      gccagagct ttagcatgc gaatcctcg tccacgctc 
      atcaacgtc accagtagc gcatttatc agcaaatgg 
      cgcggagag tcggaaaaa atggttaaa gtactattc 
      gacgttgcc aaattctat tcaccaacg acgattttc 
      atcgacgaa atcgacgcc ctcgcctcc acatcgcac 
      gattcgaat cacgaagct tcgcgtcgt ttcaaaagc 
      gagcttttg acacagatc gatggtatt atgaaaact 
      gaagtattt gttttggcc accacaaat aatccctgg 
      agtatagat aacgcgctt ttgagacgg tttgaaaaa 
      cgaattttg gtgccacta ccagataaa gactcaaga 
      agtgaattg tttcaatac tacttttcc aaaaatgga 
      tacgatttt agaaacgaa gacttggcg gtgtttgtt 
      aatcagact gaaaatttc tcaggctct gatgtgaaa 
      aacgtgtgc aaggaagct gaaatgatt gtaattcgg 
      gaaaaactg gaacaaatc agaagaggg gataagaaa 
      aagtgtacg agacaggtg aaaagtggg gacgtttta 
      aacgctctc aagaaaatc aaaccgtgt attgaacaa 
      aatgattac aagaagtat cttgactgg gggcggaaa 
      tatggggcc acgtga
    mRNA sequence
    • >TC009993 RA
      actcaatgg tgtgtggta gagaagtgg ttacatata 
      aagcaacac gctgttaat ttattacta aaaatgatg 
      gaaatccat ctacctatc aaaaacagt tggaaaacc 
      gtcaataac ttgaaagaa aacttgaaa aaagaggcc 
      caagagcgc aagcgaaac attctccac ctaatcttc 
      aaatacctc gtcgacaac aacttactc tcagtcgcc 
      gaagtgctt caaaacgaa acccaaacc ggaatccac 
      taccaagtc tgcgacaac atcgacttg gagatagtc 
      ctccaagaa taccaaagt tactattac acaaaattc 
      caaaaacaa ccaaaaatc ttacgaaaa actgccgaa 
      aacgaacaa gtggcccct ttaaaaaaa gagcgaaaa 
      tcaacagcc aaagaaaaa caaacccta aagcaagaa 
      aaaaatgac gacactttc caattcgaa attgtcaca 
      ttatcaaaa gactcgagc ttgagcgac atccagtca 
      aaaccgctg atcgatttt gaaaactac tcatcagaa 
      tggcgcgaa atggccgac caaataatc aaacaaatc 
      gtaccgaaa acactgggg ataactttc aaggattgt 
      atcagcctc agctccact attgagacg ctgaaagag 
      gcgataatt taccccttg agctacccc cagctgttt 
      gaaaaagcc tgcacttgg cgaggcgtc ctcctctac 
      ggccctcca ggcaccggc aaaacctta ctagccaga 
      gctttagca tgcgaatcc tcgtccacg ctcatcaac 
      gtcaccagt agcgcattt atcagcaaa tggcgcgga 
      gagtcggaa aaaatggtt aaagtacta ttcgacgtt 
      gccaaattc tattcacca acgacgatt ttcatcgac 
      gaaatcgac gccctcgcc tccacatcg cacgattcg 
      aatcacgaa gcttcgcgt cgtttcaaa agcgagctt 
      ttgacacag atcgatggt attatgaaa actgaagta 
      tttgttttg gccaccaca aataatccc tggagtata 
      gataacgcg cttttgaga cggtttgaa aaacgaatt 
      ttggtgcca ctaccagat aaagactca agaagtgaa 
      ttgtttcaa tactacttt tccaaaaat ggatacgat 
      tttagaaac gaagacttg gcggtgttt gttaatcag 
      actgaaaat ttctcaggc tctgatgtg aaaaacgtg 
      tgcaaggaa gctgaaatg attgtaatt cgggaaaaa 
      ctggaacaa atcagaaga ggggataag aaaaagtgt 
      acgagacag gtgaaaagt ggggacgtt ttaaacgct 
      ctcaagaaa atcaaaccg tgtattgaa caaaatgat 
      tacaagaag tatcttgac tgggggcgg aaatatggg 
      gccacgtga cagtgaatt taatgtaat taataatta 
      ttcaagaat cttcataaa tgtaacgac tacgagtat 
      aataaaagt cccaaaact aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY