General information | |||
TC033678
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 7236967..7272971 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC033678 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06277 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04693 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature posteriorpattern increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
larva dorsal pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033678 RA
MLFFLLATI LLSHAQAHD GLTSPHERA NNSLFVSKP RPRNDTFID DQFDYLVRD KRDWDEDNA SYINGSGNV TFSEQEFID SLWELIAPK SWTWILVIL HSLVFIIGI IGNILVCVA VYRNHTMRT VTNYFIVNL AVADFLVIL FCLPPSVVW DVTVTWFFG VTMCKIVLY FQSVSVTVS VLTLTFISI DRWYAICFP LKFKSTTGR AKTAIGIIW IVALACDIP EMIYVTTIP TVDEVDTVL LTQCAPTWS TETDTIFFI LKMVLFYLI PLLFMSIAY LQIIRVLWK SGNVPHQIM DASGGGGRQ TNTFAMNMN ASTEGQLRS RRKAAKMLV AVVVMFAFC YFPVHLLSI LRKTVGLKN TDGNRAFSL ISHWLCYAN SAVNPIIYN FMSGKFRKE FHRAFEHCC QRSGGHGFQ FSAVYRKTE KDSGIASRT HSRTDLEIQ RVNDFEPRH NRKGTKTSM LLVET
- >TC033678 RB
MKSVSVTVS VLTLTFISI DRWYAICFP LKFKSTTGR AKTAIGIIW IVALACDIP EMIYVTTIP TVDEVDTVL LTQCAPTWS TETDTIFFI LKMVLFYLI PLLFMSIAY LQIIRVLWK SGNVPHQIM DASGGGGRQ TNTFAMNMN ASTEGQLRS RRKAAKMLV AVVVMFAFC YFPVHLLSI LRKTVGLKN TDGNRAFSL ISHWLCYAN SAVNPIIYN FMSGKFRKE FHRAFEHCC QRSGGHGFQ FSAVYRKTE KDSGIASRT HSRTDLEIQ RVNDFEPRH NRKGTKTSM LLVET
- >TC033678 RA
atgctgttt tttctcctc gcgaccatc ctcctgtcc cacgctcaa gctcacgac ggccttaca agtcctcac gaacgagcg aataactcg ttattcgtt tcaaaaccg cgaccgaga aacgacacc ttcatcgac gaccaattc gattatctc gtgcgcgat aaaagggat tgggatgag gacaacgcc agttacatc aatgggagt ggtaatgtg acgttttcc gaacaggag ttcatcgac agcctttgg gaactgatc gcgcctaag agctggacc tggatcctg gttattctg cactctctc gtttttatc atcgggatt attgggaac attctggtg tgtgtcgcg gtctacagg aaccacacg atgcggact gtcaccaat tactttatc gtgaacttg gccgttgcc gattttctt gttattttg ttctgtttg ccgccgtcc gtcgtctgg gacgtcacc gtcacgtgg ttcttcggc gtcaccatg tgcaagatc gtgctctac ttccagagt gtatccgtg acggtgtcg gtattaacg ttgactttc atatcaatc gatcgttgg tacgccatc tgcttccct ttgaagttc aaatctacg actgggagg gccaaaact gcgatcggc attatctgg attgtggct ctggcctgt gatatcccg gaaatgatc tacgtgacg acaataccc accgttgat gaagtagac actgtgctt ttgacccaa tgcgccccg acttggtcc acggaaaca gacacgatt ttctttata ttgaagatg gttctgttc tatttgatt ccactgctc ttcatgtcg atcgcctat ctgcaaata atccgcgtg ttgtggaaa tccggcaac gttccgcat caaataatg gatgcgtct gggggcggc ggtaggcag accaacacg ttcgccatg aacatgaac gccagcacc gaaggccaa ctgcggtcg cggcggaaa gccgctaag atgctcgta gctgtcgtc gtcatgttc gctttctgc tattttccc gtacatctg ctctctatc ttaaggaaa accgtcggc ttgaaaaac acggacggc aaccgggcg ttttctttg atatcgcac tggttgtgt tacgccaac agcgccgtc aatcccatc atttacaac ttcatgagc ggcaaattc cgaaaagaa ttccaccgc gctttcgag cactgttgc cagcggtcg ggcggccat ggattccaa tttagcgcc gtgtatcgc aaaacggaa aaggactca ggaattgcc tcacgaacc cattcgagg accgacctg gagatacag cgtgtgaac gatttcgag ccgagacat aacaggaaa gggacaaaa actagcatg ttactagtg gagacctaa
- >TC033678 RB
atgaaaagt gtatccgtg acggtgtcg gtattaacg ttgactttc atatcaatc gatcgttgg tacgccatc tgcttccct ttgaagttc aaatctacg actgggagg gccaaaact gcgatcggc attatctgg attgtggct ctggcctgt gatatcccg gaaatgatc tacgtgacg acaataccc accgttgat gaagtagac actgtgctt ttgacccaa tgcgccccg acttggtcc acggaaaca gacacgatt ttctttata ttgaagatg gttctgttc tatttgatt ccactgctc ttcatgtcg atcgcctat ctgcaaata atccgcgtg ttgtggaaa tccggcaac gttccgcat caaataatg gatgcgtct gggggcggc ggtaggcag accaacacg ttcgccatg aacatgaac gccagcacc gaaggccaa ctgcggtcg cggcggaaa gccgctaag atgctcgta gctgtcgtc gtcatgttc gctttctgc tattttccc gtacatctg ctctctatc ttaaggaaa accgtcggc ttgaaaaac acggacggc aaccgggcg ttttctttg atatcgcac tggttgtgt tacgccaac agcgccgtc aatcccatc atttacaac ttcatgagc ggcaaattc cgaaaagaa ttccaccgc gctttcgag cactgttgc cagcggtcg ggcggccat ggattccaa tttagcgcc gtgtatcgc aaaacggaa aaggactca ggaattgcc tcacgaacc cattcgagg accgacctg gagatacag cgtgtgaac gatttcgag ccgagacat aacaggaaa gggacaaaa actagcatg ttactagtg gagacctaa
- >TC033678 RA
gtccggaag cgaaggaaa gtcgcagga aattggtgc atgaactgt cttcgccga tgacgaagg ttgcctcca gacaaagca atacgtaaa acctattca ccatgctgt tttttctcc tcgcgacca tcctcctgt cccacgctc aagctcacg acggcctta caagtcctc acgaacgag cgaataact cgttattcg tttcaaaac cgcgaccga gaaacgaca ccttcatcg acgaccaat tcgattatc tcgtgcgcg ataaaaggg attgggatg aggacaacg ccagttaca tcaatggga gtggtaatg tgacgtttt ccgaacagg agttcatcg acagccttt gggaactga tcgcgccta agagctgga cctggatcc tggttattc tgcactctc tcgttttta tcatcggga ttattggga acattctgg tgtgtgtcg cggtctaca ggaaccaca cgatgcgga ctgtcacca attacttta tcgtgaact tggccgttg ccgattttc ttgttattt tgttctgtt tgccgccgt ccgtcgtct gggacgtca ccgtcacgt ggttcttcg gcgtcacca tgtgcaaga tcgtgctct acttccaga gtgtatccg tgacggtgt cggtattaa cgttgactt tcatatcaa tcgatcgtt ggtacgcca tctgcttcc ctttgaagt tcaaatcta cgactggga gggccaaaa ctgcgatcg gcattatct ggattgtgg ctctggcct gtgatatcc cggaaatga tctacgtga cgacaatac ccaccgttg atgaagtag acactgtgc ttttgaccc aatgcgccc cgacttggt ccacggaaa cagacacga ttttcttta tattgaaga tggttctgt tctatttga ttccactgc tcttcatgt cgatcgcct atctgcaaa taatccgcg tgttgtgga aatccggca acgttccgc atcaaataa tggatgcgt ctgggggcg gcggtaggc agaccaaca cgttcgcca tgaacatga acgccagca ccgaaggcc aactgcggt cgcggcgga aagccgcta agatgctcg tagctgtcg tcgtcatgt tcgctttct gctattttc ccgtacatc tgctctcta tcttaagga aaaccgtcg gcttgaaaa acacggacg gcaaccggg cgttttctt tgatatcgc actggttgt gttacgcca acagcgccg tcaatccca tcatttaca acttcatga gcggcaaat tccgaaaag aattccacc gcgctttcg agcactgtt gccagcggt cgggcggcc atggattcc aatttagcg ccgtgtatc gcaaaacgg aaaaggact caggaattg cctcacgaa cccattcga ggaccgacc tggagatac agcgtgtga acgatttcg agccgagac ataacagga aagggacaa aaactagca tgttactag tggagacct aaaatattt cgcaccata tcaatacgc ggttggagt gctcacttt attcgagga caatcagga gaaaacagt acactgtga tgtaattaa atttgtgca gtcggaagc gtttttaat gattccgac tgtgctgcg gctcacctt ctgtatcaa aatgagtgt tgttacttt taaaactct gacggggga ataataaac gctgaaact tgta
- >TC033678 RB
ctcagttaa aatgaaaag tgtatccgt gacggtgtc ggtattaac gttgacttt catatcaat cgatcgttg gtacgccat ctgcttccc tttgaagtt caaatctac gactgggag ggccaaaac tgcgatcgg cattatctg gattgtggc tctggcctg tgatatccc ggaaatgat ctacgtgac gacaatacc caccgttga tgaagtaga cactgtgct tttgaccca atgcgcccc gacttggtc cacggaaac agacacgat tttctttat attgaagat ggttctgtt ctatttgat tccactgct cttcatgtc gatcgccta tctgcaaat aatccgcgt gttgtggaa atccggcaa cgttccgca tcaaataat ggatgcgtc tgggggcgg cggtaggca gaccaacac gttcgccat gaacatgaa cgccagcac cgaaggcca actgcggtc gcggcggaa agccgctaa gatgctcgt agctgtcgt cgtcatgtt cgctttctg ctattttcc cgtacatct gctctctat cttaaggaa aaccgtcgg cttgaaaaa cacggacgg caaccgggc gttttcttt gatatcgca ctggttgtg ttacgccaa cagcgccgt caatcccat catttacaa cttcatgag cggcaaatt ccgaaaaga attccaccg cgctttcga gcactgttg ccagcggtc gggcggcca tggattcca atttagcgc cgtgtatcg caaaacgga aaaggactc aggaattgc ctcacgaac ccattcgag gaccgacct ggagataca gcgtgtgaa cgatttcga gccgagaca taacaggaa agggacaaa aactagcat gttactagt ggagaccta aaatatttc gcaccatat caatacgcg gttggagtg ctcacttta ttcgaggac aatcaggag aaaacagta cactgtgat gtaattaaa tttgtgcag tcggaagcg tttttaatg attccgact gtgctgcgg ctcaccttc tgtatcaaa atgagtgtt gttactttt aaaactctg acgggggaa taataaacg ctgaaactt gta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
