Status
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Gene information
General information
TC033678
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009750,TC009749 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7236967..7272971
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033678
    iBeetle screen iB_06277

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature posteriorpattern increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva dorsal pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033678 RA
      MLFFLLATI LLSHAQAHD GLTSPHERA NNSLFVSKP 
RPRNDTFID DQFDYLVRD KRDWDEDNA SYINGSGNV 
TFSEQEFID SLWELIAPK SWTWILVIL HSLVFIIGI 
IGNILVCVA VYRNHTMRT VTNYFIVNL AVADFLVIL 
FCLPPSVVW DVTVTWFFG VTMCKIVLY FQSVSVTVS 
VLTLTFISI DRWYAICFP LKFKSTTGR AKTAIGIIW 
IVALACDIP EMIYVTTIP TVDEVDTVL LTQCAPTWS 
TETDTIFFI LKMVLFYLI PLLFMSIAY LQIIRVLWK 
SGNVPHQIM DASGGGGRQ TNTFAMNMN ASTEGQLRS 
RRKAAKMLV AVVVMFAFC YFPVHLLSI LRKTVGLKN 
TDGNRAFSL ISHWLCYAN SAVNPIIYN FMSGKFRKE 
FHRAFEHCC QRSGGHGFQ FSAVYRKTE KDSGIASRT 
HSRTDLEIQ RVNDFEPRH NRKGTKTSM LLVET
    • >TC033678 RB
      MKSVSVTVS VLTLTFISI DRWYAICFP LKFKSTTGR 
AKTAIGIIW IVALACDIP EMIYVTTIP TVDEVDTVL 
LTQCAPTWS TETDTIFFI LKMVLFYLI PLLFMSIAY 
LQIIRVLWK SGNVPHQIM DASGGGGRQ TNTFAMNMN 
ASTEGQLRS RRKAAKMLV AVVVMFAFC YFPVHLLSI 
LRKTVGLKN TDGNRAFSL ISHWLCYAN SAVNPIIYN 
FMSGKFRKE FHRAFEHCC QRSGGHGFQ FSAVYRKTE 
KDSGIASRT HSRTDLEIQ RVNDFEPRH NRKGTKTSM 
LLVET
    CDS sequence
    • >TC033678 RA
      atgctgttt tttctcctc gcgaccatc ctcctgtcc 
cacgctcaa gctcacgac ggccttaca agtcctcac 
gaacgagcg aataactcg ttattcgtt tcaaaaccg 
cgaccgaga aacgacacc ttcatcgac gaccaattc 
gattatctc gtgcgcgat aaaagggat tgggatgag 
gacaacgcc agttacatc aatgggagt ggtaatgtg 
acgttttcc gaacaggag ttcatcgac agcctttgg 
gaactgatc gcgcctaag agctggacc tggatcctg 
gttattctg cactctctc gtttttatc atcgggatt 
attgggaac attctggtg tgtgtcgcg gtctacagg 
aaccacacg atgcggact gtcaccaat tactttatc 
gtgaacttg gccgttgcc gattttctt gttattttg 
ttctgtttg ccgccgtcc gtcgtctgg gacgtcacc 
gtcacgtgg ttcttcggc gtcaccatg tgcaagatc 
gtgctctac ttccagagt gtatccgtg acggtgtcg 
gtattaacg ttgactttc atatcaatc gatcgttgg 
tacgccatc tgcttccct ttgaagttc aaatctacg 
actgggagg gccaaaact gcgatcggc attatctgg 
attgtggct ctggcctgt gatatcccg gaaatgatc 
tacgtgacg acaataccc accgttgat gaagtagac 
actgtgctt ttgacccaa tgcgccccg acttggtcc 
acggaaaca gacacgatt ttctttata ttgaagatg 
gttctgttc tatttgatt ccactgctc ttcatgtcg 
atcgcctat ctgcaaata atccgcgtg ttgtggaaa 
tccggcaac gttccgcat caaataatg gatgcgtct 
gggggcggc ggtaggcag accaacacg ttcgccatg 
aacatgaac gccagcacc gaaggccaa ctgcggtcg 
cggcggaaa gccgctaag atgctcgta gctgtcgtc 
gtcatgttc gctttctgc tattttccc gtacatctg 
ctctctatc ttaaggaaa accgtcggc ttgaaaaac 
acggacggc aaccgggcg ttttctttg atatcgcac 
tggttgtgt tacgccaac agcgccgtc aatcccatc 
atttacaac ttcatgagc ggcaaattc cgaaaagaa 
ttccaccgc gctttcgag cactgttgc cagcggtcg 
ggcggccat ggattccaa tttagcgcc gtgtatcgc 
aaaacggaa aaggactca ggaattgcc tcacgaacc 
cattcgagg accgacctg gagatacag cgtgtgaac 
gatttcgag ccgagacat aacaggaaa gggacaaaa 
actagcatg ttactagtg gagacctaa
    • >TC033678 RB
      atgaaaagt gtatccgtg acggtgtcg gtattaacg 
ttgactttc atatcaatc gatcgttgg tacgccatc 
tgcttccct ttgaagttc aaatctacg actgggagg 
gccaaaact gcgatcggc attatctgg attgtggct 
ctggcctgt gatatcccg gaaatgatc tacgtgacg 
acaataccc accgttgat gaagtagac actgtgctt 
ttgacccaa tgcgccccg acttggtcc acggaaaca 
gacacgatt ttctttata ttgaagatg gttctgttc 
tatttgatt ccactgctc ttcatgtcg atcgcctat 
ctgcaaata atccgcgtg ttgtggaaa tccggcaac 
gttccgcat caaataatg gatgcgtct gggggcggc 
ggtaggcag accaacacg ttcgccatg aacatgaac 
gccagcacc gaaggccaa ctgcggtcg cggcggaaa 
gccgctaag atgctcgta gctgtcgtc gtcatgttc 
gctttctgc tattttccc gtacatctg ctctctatc 
ttaaggaaa accgtcggc ttgaaaaac acggacggc 
aaccgggcg ttttctttg atatcgcac tggttgtgt 
tacgccaac agcgccgtc aatcccatc atttacaac 
ttcatgagc ggcaaattc cgaaaagaa ttccaccgc 
gctttcgag cactgttgc cagcggtcg ggcggccat 
ggattccaa tttagcgcc gtgtatcgc aaaacggaa 
aaggactca ggaattgcc tcacgaacc cattcgagg 
accgacctg gagatacag cgtgtgaac gatttcgag 
ccgagacat aacaggaaa gggacaaaa actagcatg 
ttactagtg gagacctaa
    mRNA sequence
    • >TC033678 RA
      gtccggaag cgaaggaaa gtcgcagga aattggtgc 
atgaactgt cttcgccga tgacgaagg ttgcctcca 
gacaaagca atacgtaaa acctattca ccatgctgt 
tttttctcc tcgcgacca tcctcctgt cccacgctc 
aagctcacg acggcctta caagtcctc acgaacgag 
cgaataact cgttattcg tttcaaaac cgcgaccga 
gaaacgaca ccttcatcg acgaccaat tcgattatc 
tcgtgcgcg ataaaaggg attgggatg aggacaacg 
ccagttaca tcaatggga gtggtaatg tgacgtttt 
ccgaacagg agttcatcg acagccttt gggaactga 
tcgcgccta agagctgga cctggatcc tggttattc 
tgcactctc tcgttttta tcatcggga ttattggga 
acattctgg tgtgtgtcg cggtctaca ggaaccaca 
cgatgcgga ctgtcacca attacttta tcgtgaact 
tggccgttg ccgattttc ttgttattt tgttctgtt 
tgccgccgt ccgtcgtct gggacgtca ccgtcacgt 
ggttcttcg gcgtcacca tgtgcaaga tcgtgctct 
acttccaga gtgtatccg tgacggtgt cggtattaa 
cgttgactt tcatatcaa tcgatcgtt ggtacgcca 
tctgcttcc ctttgaagt tcaaatcta cgactggga 
gggccaaaa ctgcgatcg gcattatct ggattgtgg 
ctctggcct gtgatatcc cggaaatga tctacgtga 
cgacaatac ccaccgttg atgaagtag acactgtgc 
ttttgaccc aatgcgccc cgacttggt ccacggaaa 
cagacacga ttttcttta tattgaaga tggttctgt 
tctatttga ttccactgc tcttcatgt cgatcgcct 
atctgcaaa taatccgcg tgttgtgga aatccggca 
acgttccgc atcaaataa tggatgcgt ctgggggcg 
gcggtaggc agaccaaca cgttcgcca tgaacatga 
acgccagca ccgaaggcc aactgcggt cgcggcgga 
aagccgcta agatgctcg tagctgtcg tcgtcatgt 
tcgctttct gctattttc ccgtacatc tgctctcta 
tcttaagga aaaccgtcg gcttgaaaa acacggacg 
gcaaccggg cgttttctt tgatatcgc actggttgt 
gttacgcca acagcgccg tcaatccca tcatttaca 
acttcatga gcggcaaat tccgaaaag aattccacc 
gcgctttcg agcactgtt gccagcggt cgggcggcc 
atggattcc aatttagcg ccgtgtatc gcaaaacgg 
aaaaggact caggaattg cctcacgaa cccattcga 
ggaccgacc tggagatac agcgtgtga acgatttcg 
agccgagac ataacagga aagggacaa aaactagca 
tgttactag tggagacct aaaatattt cgcaccata 
tcaatacgc ggttggagt gctcacttt attcgagga 
caatcagga gaaaacagt acactgtga tgtaattaa 
atttgtgca gtcggaagc gtttttaat gattccgac 
tgtgctgcg gctcacctt ctgtatcaa aatgagtgt 
tgttacttt taaaactct gacggggga ataataaac 
gctgaaact tgta
    • >TC033678 RB
      ctcagttaa aatgaaaag tgtatccgt gacggtgtc 
ggtattaac gttgacttt catatcaat cgatcgttg 
gtacgccat ctgcttccc tttgaagtt caaatctac 
gactgggag ggccaaaac tgcgatcgg cattatctg 
gattgtggc tctggcctg tgatatccc ggaaatgat 
ctacgtgac gacaatacc caccgttga tgaagtaga 
cactgtgct tttgaccca atgcgcccc gacttggtc 
cacggaaac agacacgat tttctttat attgaagat 
ggttctgtt ctatttgat tccactgct cttcatgtc 
gatcgccta tctgcaaat aatccgcgt gttgtggaa 
atccggcaa cgttccgca tcaaataat ggatgcgtc 
tgggggcgg cggtaggca gaccaacac gttcgccat 
gaacatgaa cgccagcac cgaaggcca actgcggtc 
gcggcggaa agccgctaa gatgctcgt agctgtcgt 
cgtcatgtt cgctttctg ctattttcc cgtacatct 
gctctctat cttaaggaa aaccgtcgg cttgaaaaa 
cacggacgg caaccgggc gttttcttt gatatcgca 
ctggttgtg ttacgccaa cagcgccgt caatcccat 
catttacaa cttcatgag cggcaaatt ccgaaaaga 
attccaccg cgctttcga gcactgttg ccagcggtc 
gggcggcca tggattcca atttagcgc cgtgtatcg 
caaaacgga aaaggactc aggaattgc ctcacgaac 
ccattcgag gaccgacct ggagataca gcgtgtgaa 
cgatttcga gccgagaca taacaggaa agggacaaa 
aactagcat gttactagt ggagaccta aaatatttc 
gcaccatat caatacgcg gttggagtg ctcacttta 
ttcgaggac aatcaggag aaaacagta cactgtgat 
gtaattaaa tttgtgcag tcggaagcg tttttaatg 
attccgact gtgctgcgg ctcaccttc tgtatcaaa 
atgagtgtt gttactttt aaaactctg acgggggaa 
taataaacg ctgaaactt gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY