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Gene information
General information
TC009611 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3247566..3251396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009611
    iBeetle screen iB_06275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009611 RA
      MDGTFKFAD FIAGWFGGI CGVVVGHPL DTIKVRQQN 
      FGTKLFVAI SRTFRHEGI PGFFKGMLC PVLTIGPSN 
      AILFGVYGN LMNVFNENY VPRQVSHTD FDALRHVFI 
      AGTIGGFFQ SLFVCPAEL VKTLMQIKT EGKGSWRRH 
      SEVVYTGSI DAFFGIVRD RGFRGLFRG FAPMAIRDV 
      PTSGLYTVT YEALNSYFE QCHVPLMLK QTIAGGTAG 
      VASWILVIP FDVVKSRIQ ADSYNHPQY KGMIDCFYK 
      SYQRDGLGI FFKGAPAIV MRTFPVNAA LFVGYEAVL 
      RHL
    CDS sequence
    • >TC009611 RA
      atggatggg acgttcaag ttcgccgat ttcattgca 
      ggatggttc ggaggcatc tgtggggtg gtggtgggc 
      caccccctc gacacgatc aaagtgcgc caacagaac 
      ttcggcacg aagctgttc gttgccatt tcgcgcaca 
      ttccggcac gagggaatc cccggcttc ttcaaagga 
      atgctatgt cctgtactt accatcggc ccctcgaac 
      gccattctt ttcggcgtt tacggcaac ttaatgaac 
      gtcttcaac gaaaactac gtacctcgc caagtctcg 
      cacaccgac ttcgatgcc ctaagacac gttttcatc 
      gccgggaca attgggggc ttctttcaa tcgcttttc 
      gtgtgtccg gctgaactg gtcaagacg ctgatgcaa 
      atcaagacg gagggcaaa ggctcatgg agacgccac 
      tcggaggtg gtttacacg ggatcgatt gacgcgttt 
      ttcgggatt gtgcgggat cgggggttc agagggctc 
      ttcaggggc ttcgccccc atggctatc agggatgtg 
      cccactagc ggtttgtac acggtgacc tatgaggct 
      ttaaattcg tattttgag cagtgccat gtgcccctt 
      atgctcaag cagaccata gctgggggc actgcaggg 
      gtggcttcc tggattttg gtgattccc tttgatgtg 
      gtgaagtcc cgaatccag gccgatagt tacaatcac 
      cctcaatat aaaggaatg atcgactgt ttttacaaa 
      tcgtatcaa agggacggt ttagggatt ttttttaaa 
      ggcgcccct gcgatagtc atgaggact ttccctgta 
      aatgcggcg ctatttgtg ggctatgag gctgtgcta 
      agacatctg taa
    mRNA sequence
    • >TC009611 RA
      tttgccgac caccccttc cttgatcgt tttcaaatc 
      tttgttttt gcgcgatag catggtcag caggtgtgc 
      aaattgcgg gctattttc ggctgaaaa ttgagggcc 
      agtcgatga gggtttgcg acggcggct ggcggaggc 
      gtctcaagc gatggatgg gacgttcaa gttcgccga 
      tttcattgc aggatggtt cggaggcat ctgtggggt 
      ggtggtggg ccaccccct cgacacgat caaagtgcg 
      ccaacagaa cttcggcac gaagctgtt cgttgccat 
      ttcgcgcac attccggca cgagggaat ccccggctt 
      cttcaaagg aatgctatg tcctgtact taccatcgg 
      cccctcgaa cgccattct tttcggcgt ttacggcaa 
      cttaatgaa cgtcttcaa cgaaaacta cgtacctcg 
      ccaagtctc gcacaccga cttcgatgc cctaagaca 
      cgttttcat cgccgggac aattggggg cttctttca 
      atcgctttt cgtgtgtcc ggctgaact ggtcaagac 
      gctgatgca aatcaagac ggagggcaa aggctcatg 
      gagacgcca ctcggaggt ggtttacac gggatcgat 
      tgacgcgtt tttcgggat tgtgcggga tcgggggtt 
      cagagggct cttcagggg cttcgcccc catggctat 
      cagggatgt gcccactag cggtttgta cacggtgac 
      ctatgaggc tttaaattc gtattttga gcagtgcca 
      tgtgcccct tatgctcaa gcagaccat agctggggg 
      cactgcagg ggtggcttc ctggatttt ggtgattcc 
      ctttgatgt ggtgaagtc ccgaatcca ggccgatag 
      ttacaatca ccctcaata taaaggaat gatcgactg 
      tttttacaa atcgtatca aagggacgg tttagggat 
      tttttttaa aggcgcccc tgcgatagt catgaggac 
      tttccctgt aaatgcggc gctatttgt gggctatga 
      ggctgtgct aagacatct gtaataaaa ataaatcac 
      acgtgactt accttgatt tttatttct ggtttttgc 
      atcagtaat agacaattt aaaaaaaat ataaattgt 
      tgaaatttg gccaatatc tcaaaaaaa attatgata 
      agtaaaaac ccgcggtaa aaactaatt ccaacaacg 
      atttgacga attttaaaa aagaaagct ctgaaagtg 
      agtaaactg attgtatct tcaactgta ttcgcagcg 
      ttcgtaaaa atataaagt agaaggtaa attgataat 
      ttgttgatt tgtgtgacc ataacgggc gacttacct 
      tgactgagg ttttgaagt gtgaaacag aaaaaaaaa 
      ttcaaaatg acaatattt attgacgca caacagtgc 
      gtacattca caaactgca gttactata atcctcaat 
      aacgaggtt tttataagt agctcaata gaaattact 
      atgaaaggt ttataaatg ttccatttt acactcaca 
      aaatcttaa taagaaaaa taacagaac agcggtccg 
      agaacctat gagaaaaac aatcgaaat ttaacaata 
      tatttctca tatatctac aagatttat agtgatttt 
      agttatata ttacatact ttagtctaa tgagggatg 
      ggcgtaata gtgtggccc taatgtttg tttcataca 
      gcagcaaag gttatgcat cggtgttcg tagagctac 
      cgatcctct ctggaattg ttttatcac aacataact 
      ctacgaaca taacatttc caggtcgca gctagaaat 
      tgagcgagc tcgtcatgc acaacttaa ttattttct 
      atctaaaac acaacttgc tatatttcc ttatataaa 
      gctttctct aagtgaaca taacagtgc gtctaaaaa 
      agtttggta gtgaagtgg caccattgt atagaaaag 
      tcctggctg tataatttg tgacctttg cttcgcaat 
      aaataaaga agaaagtaa aacaaaaca aaaattgca 
      ctgtacaac aatccgtga aactaatac aaaacgagt 
      ctagatgag tgactaact tatcctacg attagttca 
      agttataca gccatctgt acataaata ttacattcg 
      ccccttcct ggcacacga tcgtgcgat tagcacata 
      caaaataac tgtggttaa aactgtgtt tggacgtga 
      cacaatttt aactggttt gttttttgg tcgattagg 
      tcctccagc ccaaagtta tttcggtta actgacatc 
      gaaagctgc aaaaaataa ctttggcac ttgtttgtt 
      ttaaaacac aacgaaatt ttttcaacg atttattat 
      tcaaatcaa atacactaa gtcaccaac cacggtcca 
      aacacaaac cgtaaccac atctgctta gctatttac 
      agttgacca gttatacta tagcacatt attaacata 
      gttctgctg ttcacagct acaaaacac catagatag 
      actgttagt agtagacat tcacgataa ttttaatgt 
      acactccgg ctcatgacg agacgacga cgacggctc 
      tctccacgt gcgggggca cctttcaca ctgaaatat 
      gccactaca ccaaaaact gaccacact aacactctc 
      aaagctcga aaacaccga tttttgtaa aattgaaca 
      attataaaa attaccatc ttaataaac ccacttcaa 
      ataggcagt ctagttttg gattttttt tctttaaat 
      attgtctaa tttcaaatt ttttgtata aattggcaa 
      tgaaatctc ctaaacgag gctaaaaac ttgtaaatt 
      aacgataat aagacaacg taataatat gatttgaaa 
      caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY