General information | |||
TC033570
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2788712..2815636 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC033570 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04659 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment dorsoventral-axis thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06274 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Comment: Femur and Tibiotarsus potentially fused. |
thorax circumference irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax circumference thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen segment number decreased -
abdomen circumference irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen circumference thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC033570 RA
MMGIPASGP ALKECPCEE VDVGFECRC SGPALMDIP NSLHKELTI LVIEDAGIE VLKSDSLVP YSSTLRELY IHRAPKLLK IQPEVFSAY LPNLKILRI VNTGLEEMP NFNGLNTSS VLHMIDLEH NRIQRINTR QISRVKAEQ LVLNYNDLQ VVEEAAFAG SQIAKLSLS GNFRLKNLH VKAFHGLES LRYIDLSST AITALPVEG LREIDVLKV QDTESLKVF PSVYNFKYI KEAWLTYPY HCCAFKFPH THNPWEYER HNRFVKQQR QACTAKNAK NADPTLRAL FRAIPPNAS LAESDFDEQ WNGDEIFHN STMNLNNGS PVTTAVCGE IYRNYHEVK CHPAPDAFN PCEDLMGNW ALRIPVWFI SLSAVIGNL FVVIVISTS HFRLTVSKF LMCNLAVAD LCIGIHLLL IAVVDACSI RAYFNYAID WQEGNGCRV AGTLTIFGN VLSVYTLAV ITTERWYTI TWAIHLNKR LKLRTAVRV MIAGWICAL FMACLPLVG VSSYSKTSI CLPLENKGK ADTIYLSTL LAFNAIAFA LICVCYGSM YKSIREGGH TGSTTSVRS DLTVAKRMA LLVFTDFAC WAPIAFFSL TALLGHPLI TVTQTKILL VFFYPLNSC ANPCLYALL TQQYRRDFF ILMGRYGLC KDRAERHKG AIGGKPLPY GSGPQYKRT SGEAAGKIH SAGGNNHRG SLLTTIVSV EVPMVRTSS RNSARSRPD SLNAVPEYS LQQINLGMM DDCRKLTST DNFCLSQQS DTCYQTKPF LLLISECFF PQCLIPTRP DMDQLITSL DKLLKYKNE DPSRFYTFL SDCTETDEK QWESLIPKI YYLYDRQVL RKDLVYLSL LASQNHALL RKFVDYDKS IYVLNVADP VSLYDYYVY KGKHWVYEI IDNPGNHAK LVDLKNIYN KFQMIKYVK ETNTSVVDL DQLERLSHA INFEFLDDF WKFYVAEIT RFITTVKFC KTLKPDAND SDLFVFASK NTQLDTLSR FTDLSDATQ WENILAKLC NNCDQFNIF TGFVILATL INLLIDNYN TNLKKDHVN ALFETVKTK LIDLKDKQL QIELLENIF RLMFHRKSH EFTCKEKEI RFVLFLLKS VTEELKLKK VYERDSEHY RRLSTLSIY VTDALWRLD LILNTKNTP KIQEQLLNY MLAPPESLI HICLKRHNF ERAHQVIEI FDLRDSDLA QQVNYSETF LKFRKFLRK NGKLAQTVT EFFESNSPN EIFTNIFDL AITGTQTWD ESEILLNLA EKFNSDETL PSSYLNFYK RVTEIGHEV GPKDNISLP QILISPNYP LDVNVYKTK VEFFQNLAR LYDEFNGQN RPNEPGSFN KNHPNHKTL LKMNELIVN YLQDRDTTQ YLARIFNYL KAFSKILYI EQNSSDIIS KGKNSSYFD IFNFNRSEL TGKLLFERQ LDPTEFEKY FGKLKLDFL YHVVGNCFP TINLHVQEN VTIEELYHD NMLYIPSRS IIVYIQKRN WLLAFILNE MYKVEGVKI DISEVRLRT FFNYLKSPR IQHLKALFD KNDIIAALQ NCINAQKVK LYFRERIIE HDLVSIQSQ NSSNSDGFE TGEEIQEDA KTTNWRTLY DIIESIPEN QMKKKSDLI ICKDNVLVN MVQDGFEPE YYKYAHFIT NRDLRIDTI LSNFRKWPG KFAADIIKS EISRFDCLM DSKMETLCG WLQQIELNE ELKSVFEVK SWYKSYAFC KEVPESALE KILLTNNVD FLLDFVQLY QPSPETVCK INSSYLTRI MDTNIPLDR IKKLLDSLP FEHAIDMCH NLLTILHNL EYLQFIADY LSTNVSDDL FLKNVQISL KMLSVFTPL EQDQLFCLI NEPLSILEV LLMNTKLDK LAAILDLLN AHNLSDFSE VITTEKIDE LLRKYAEKS LDFRVITHP NPGLFKTPE PKLLQSLDS LSLMSDKRQ FVVPENVPT KEEWVQNDE VVECMCCHQ VTFSMFNRR HHCRRCGRV VCYNCSLKR MFVPTYGDI LVRVCSDCY NQTVGERND SNSDKSRSF IQEYWLLND DPEHNKIIR EEFSYEHAP STSLCFSIL RYHSKSIEY PKFLLEQCE VMLKLLQPN QEAAQEIDY LLVIKMLKS LAMAAKMVS TENMLQSGA SMADRILSQ AELLALLAE RGCLNLMPS QNLYVDANV LRRLRDKLL QREQWNLAL EVSTKAGLD NTGVFAAWS KSCLKAGSL LLAREKFQR CLDKNSQYE TSSNLSRLS ELNSSLDSL SLSRSRMSS RFSSGSFTL DNKPAKDPP LLYEIIQIL ETKTRTINP KVVEKAQSI KISGSVTSL NQISSSYTV HIDTALSIV NRVKNLKEI ASGNYSLTA SKKFNCYTS RPTIDPIFY EECIYYLGK YGTHLSLLE FYIRHGEIH TALIYILEC HLPSETFIE VYMKCLKDG IVTILQEHM SRIDSSLEL WKDHLKNLC RHLEKQQLL NCLYQLQLY IGDYVRASM TCIRFYQDN VKSFTELAN NVGFLHKGE GHLRQILEQ EQWVEVTSV TSKDSFEER LLTNSDLVM KIESKDVNR HINTVKRQV EIVKFLADC EAAGVNTRD LLRQLVLTK DPKAEQQVA IPTLFGTTL EKINLAVMA IISGETVED GFNLATKII EDFKLRPIK VYCLAGKQF AKSERYNGI TQLVNCIKS TGINDSAIS DMLDEMLAH AVGTLIKEN IEGTKVEDL IKLINDKAT KISAYIEAK QLKTAYFLA VKYKRTADI RRIQREAEL LNMPTIKAL CQKVLQNSP HTHSKD
- >TC033570 RA
atgatgggc atccccgcc agcgggccg gccctgaaa gagtgtccc tgcgaggaa gtggacgtg gggtttgaa tgcagatgc agcggtccg gccctgatg gacatcccc aacagcctg cataaagag ctcaccatt ctggttatc gaggacgcc gggattgaa gtgctgaaa agcgattcg ctcgtgccc tactcgagc acgctgcga gagctatac atccaccgc gcccccaaa ctcctgaaa atccagccg gaggttttc agcgcttac ctccccaac ctcaagatc ttgaggatc gtcaacacg ggattagag gaaatgccg aacttcaac ggattgaac acttcctcg gtgttgcac atgatagat ttggagcat aaccgcatc cagagaatc aacacgcgg caaattagc agagtcaag gctgagcaa ctagtgctc aactacaac gatttgcaa gtggtcgaa gaggcggca tttgcgggc tcgcagatc gctaaatta agcctgagt ggaaatttt cgactgaaa aacctccac gtgaaggcg tttcacgga ctggagagc ttgcgctac atcgacctc tcaagcact gccataacg gcgttaccc gtggaaggc ctgcgggaa atcgacgtc ctcaaagtc caagacaca gagtcgctt aaagtcttt ccctcagtt tacaacttc aagtacata aaagaagcc tggctgacc tacccttac cattgttgc gccttcaag ttcccccac acccacaac ccctgggag tacgaaaga cacaaccga tttgtcaag cagcagcgc caagcttgc accgccaaa aacgccaaa aacgctgac ccgactctg agggcgctg tttagggcc attccgccg aatgcaagt ctcgctgag agcgatttt gacgagcag tggaacggc gatgagatt tttcacaac agtacgatg aatctgaat aatggatcg ccggttact accgccgtt tgtggagag atatacagg aattatcac gaggtcaag tgtcatcca gcgccggac gccttcaac ccctgcgaa gacctgatg ggaaattgg gctttaagg ataccggta tggttcata tcgttatcg gcagtgatc ggaaatctg ttcgtggta atagtcatc tcgacatcg cattttcga ctgacagtt tcgaaattt ctcatgtgc aatttagcc gtggctgat ttatgcatc ggcatccat ctgctgctt attgccgtg gtggacgcc tgctccata agggcctat ttcaattac gccatcgat tggcaagaa ggcaacggc tgtcgcgtt gccggcacg ctcaccatc ttcggcaac gtcctctcg gtgtacacc ctcgctgtg ataaccacc gagcgatgg tacaccatc acctgggcc atccatctc aacaagcgc ctaaagctc cggacggca gttcgcgtg atgatcgcc ggctggatt tgtgccctt tttatggca tgtctcccc cttgtgggg gtgagcagc tactccaaa acgagcata tgtctgccc ttggagaac aaaggcaaa gccgacacg atatatctc tccactttg ctcgctttc aacgcgatc gcgttcgcc ctcatctgc gtctgctac ggaagtatg tacaagtcg attcgtgag ggtggacac acgggatct acgacttcc gtccggtcc gacctcact gtggccaag aggatggct ttgctcgtc ttcaccgat tttgcgtgc tgggcgccg atcgccttc ttcagcctc acggcgctt ttaggccat ccgttgatc accgtcacg cagacgaag atcctcctc gtgttcttc tacccgctc aactcgtgc gccaacccc tgtctgtac gccctgctg acgcagcaa taccgccgc gactttttc atcttaatg gggcgctac ggcctgtgc aaggaccgc gccgagcgc cacaagggg gccatcggg ggcaaaccg ctcccgtac ggctccggc ccgcagtat aagcggact agcggcgag gcagccggc aaaattcac agtgcggga ggtaacaac catcgaggg tctcttttg accactatc gtttcagta gaagtgccc atggtgagg actagttcc cggaacagt gcgcgctcg cgcccggac tcgctcaac gccgttccg gagtattcc ctccagcaa atcaacctc ggcatgatg gacgattgc cggaaactc acttcgacc gataacttc tgtttgtca caacaaagt gacacatgt tatcaaaca aaacctttt ttattatta atcagtgaa tgctttttt ccgcaatgt ttaatcccg actcgacct gacatggat cagttaatt acgagcctt gataagctc ctgaaatac aaaaacgag gatccctcc cgtttttac accttcttg agcgattgc accgaaacc gatgaaaaa caatgggaa agtttgata cctaaaatt tactatctg tatgataga caagtttta cgcaaagat ctcgtttat ctttcattg ttggcgagc cagaatcac gctctttta cggaaattt gttgattac gataagtct atttacgtt ttaaatgtt gccgatcca gtctcgctt tacgattat tatgtttac aaggggaaa cactgggtt tatgagata atagacaat ccgggcaat catgcgaaa cttgttgat ttgaaaaat atttacaat aaattccaa atgataaaa tacgttaaa gagacaaac acgtctgtt gttgacctc gaccagctt gagcgtctc tcccacgca ataaatttc gagtttttg gacgatttt tggaaattt tacgtcgcc gagataacg cgatttatc acaacagta aagttttgt aaaactctc aaaccggat gcaaacgat tcagattta tttgttttc gcgagtaaa aacacacaa cttgatact ttatcgcgt ttcaccgac ttgagcgat gccacacaa tgggaaaac atcttagct aaactatgc aataattgc gaccaattt aacattttc accggcttt gtgatactc gcgactttg atcaatctc ctaatagac aattacaac accaatttg aaaaaagat cacgtcaat gccctcttc gaaaccgtc aaaaccaaa ctcatcgac ttaaaagac aagcaatta cagatcgaa ttactcgag aatattttc cgcttgatg tttcaccga aagtcgcac gaattcact tgcaaagag aaagaaatc cgcttcgtt ttgttttta ctaaagtca gtaacagag gaattaaaa ttgaaaaaa gtctacgaa cgcgactcc gaacactat cgaagactc tcaactctc tcgatttac gtaacggac gccttgtgg cgtctcgac ttgatcctc aacacaaaa aacacccca aaaatacaa gaacaactc ctcaactac atgctcgca cctccggag tccctcatc cacatctgc ctcaaacgg cacaacttc gaacgcgcc caccaggtg atagagatt ttcgatctg agggactct gatctcgcg caacaagtc aactactcg gaaactttc ctcaaattc aggaaattc ctccgaaaa aacggcaaa ttggcgcaa acagtcacc gaatttttc gaatcaaat tcgccaaac gaaattttt acgaatatt ttcgatctt gcaatcacc ggaacccaa acgtgggac gaatcagaa attttgcta aacctagcc gaaaaattc aattccgac gaaacgctc ccttctagt tatctcaat ttctacaaa cgagtgacg gaaattggg cacgaagtg ggccccaaa gacaacatt tcactgccc cagattcta atttcccca aactatccc ttggatgtt aacgtctac aaaaccaaa gtggaattt ttccaaaac ttggcccga ctttatgac gaatttaac ggtcaaaac cgccctaat gaacccggg agtttcaat aaaaaccac ccaaatcac aaaaccttg ctcaaaatg aacgaactg atcgttaat tacctccaa gatcgggac acaacgcaa tacctcgcc cgcattttc aattacttg aaagccttt tcgaaaatt ttgtacatc gagcagaac agttcggac atcatttcg aagggaaaa aactcgtct tacttcgac attttcaac ttcaaccgc tcggaattg accgggaaa ttgctgttc gagcgtcag ctggacccg actgagttt gaaaaatac ttcgggaaa ctcaagttg gattttttg taccacgtg gtggggaat tgcttcccg acgattaat ctccacgtg caggaaaat gtgaccatt gaagagctg taccatgat aacatgttg tacattccg agccgcagc ataattgtg tacatccag aaacgcaac tggttgctt gcctttatc ctgaacgag atgtacaaa gttgaagga gtcaaaatc gatattagc gaagttcgc ttaaggacg ttctttaat tatttaaaa tcgcccaga atccagcac ttgaaggcg cttttcgac aaaaacgac atcatcgca gcgctccag aactgcata aatgcccaa aaagtcaag ttatatttc cgcgaacgc attatcgag catgatcta gtctcgatc cagtcgcaa aacagcagc aattctgac ggttttgaa accggggaa gagattcaa gaagacgca aaaacgacc aactggaga accctctac gatattatc gaaagcatt ccggaaaat cagatgaag aaaaagtca gacttgatc atttgcaag gacaatgtt ctggttaac atggtgcaa gacgggttc gagcccgaa tattacaaa tacgctcat tttattaca aacagggac ttgcggatt gacacgatt ttgagcaat tttaggaaa tggccgggg aaattcgcc gctgatata atcaaaagt gagattagt agattcgat tgtttgatg gattcgaaa atggagacg ttgtgtggt tggttgcaa caaattgag ctcaacgaa gagctgaaa agcgttttt gaggtgaaa tcgtggtac aagagctac gctttttgc aaggaggtg cccgaaagc gcccttgaa aaaatcctg ctaacgaat aatgtggac tttttgctg gacttcgtc cagttgtac caaccctcg cccgaaacc gtctgcaaa atcaattcg agttactta acccgaata atggacacg aacattcca ctcgaccgg ataaaaaaa ctgctcgat agtctaccc ttcgagcac gccatagac atgtgccat aacttactc acaatctta cacaacttg gaatatcta caattcatc gccgattat ctaagcacc aacgtttcg gacgactta ttcctaaag aacgtccaa atcagtttg aaaatgctc tcggtcttc acaccactc gagcaagac caacttttc tgtctcata aacgaacct ttgagcatc ctcgaagtg ctcctaatg aacacaaaa ctggacaaa ctcgcggct attttagac ttgctaaac gcccacaat ttaagcgac tttagcgag gttataacc acagagaaa atcgacgaa ttgttgcgg aaatacgcc gagaagagc ttggatttt cgcgttatc acgcaccct aatcccggc ttatttaaa acaccggag ccgaaatta ttacaatcg ttggactcg cttagttta atgtccgat aaacgccag tttgtcgtc cctgaaaac gtcccaact aaggaagag tgggttcaa aacgacgaa gtcgtcgag tgcatgtgt tgccaccag gtcactttt tccatgttc aatcggcgg caccactgt cggagatgt ggccgcgtt gtttgttac aattgttcc ttgaaacgc atgttcgtg cccacttat ggcgacatt ctagtgaga gtttgctcc gactgttat aatcagaca gtcggggag agaaacgac agcaattcg gacaaaagc cgctccttt atacaggaa tattggctc ttgaacgac gaccccgaa cataataaa atcataaga gaggagttc tcctacgaa cacgcccct agcacctct ctgtgtttc tcaatcctg agatatcac tccaaaagt atcgaatat cccaagttt ttattagag cagtgtgaa gtcatgttg aagttattg caaccgaat caagaggcg gcccaagaa attgactat ctcctcgtt ataaaaatg ttaaagtca ctcgctatg gcggccaag atggtctcg actgaaaat atgctccaa tcgggtgct tccatggcc gaccggatt ctctcacag gccgaactt ttggccctt ttggcagaa cgcggctgt ttgaatctt atgccgagt caaaatctt tacgtcgat gctaatgtt ttgcgccga ttgcgagat aaattgcta cagagggag cagtggaat ttggccctg gaggtgtcc accaaagcg ggactcgac aataccggg gtttttgcc gcctggagc aagagctgc cttaaggcg ggcagcctg ctcctagcc cgcgaaaag ttccaacgc tgccttgac aagaacagc caatacgaa acctcgtca aatttgagc cgactttcc gagctcaat tcgagttta gatagtttg agtttgagt cgatctcgg atgagttca cgattctca agcggaagt ttcacctta gacaataaa ccagctaaa gatccgccc ctcttgtac gaaatcatc caaattttg gaaaccaaa acgcggact ataaacccg aaagttgtc gaaaaagcc caaagtatc aagatttca ggctcggtc acgagcctc aaccaaata tcatcatcg tacaccgtg cacattgac acagcctta agcatcgta aaccgggtc aaaaatttg aaagaaatc gcatcgggg aattactca ctgaccgca agcaaaaaa ttcaattgt tacactagt cggcccaca atcgacccg attttttac gaggaatgt atctattat ttgggcaaa tacgggacg catttatcg ctactggag ttttatatc aggcatggg gaaatccac actgccctc atatacata ctcgagtgc catctaccg agcgaaacc ttcatcgaa gtctatatg aagtgcctt aaagacggg atcgtaaca attttgcaa gagcacatg tcacggatc gactcatcg ctcgaactg tggaaagac cacttgaag aatttgtgt cgccacttg gagaagcaa caattattg aactgtttg taccagttg cagctgtac atcggcgac tatgtgcgc gcttcgatg acttgtatc cgattctac caagacaac gtgaaaagt ttcacagaa cttgcgaac aatgtcggc tttttgcac aagggcgag gggcatttg aggcagatt ttggagcag gagcagtgg gtggaggtg acttcggtc acttccaag gatagtttc gaggagaga ctcttgacc aattcggac ttggtcatg aaaatcgag tcgaaagac gtcaaccga cacataaat acagtaaaa cgacaggtg gaaattgtg aaatttttg gccgattgt gaggcggca ggggtcaac acgcgcgac ctgcttcgg cagctggtt ttgaccaaa gatcctaaa gccgagcag caagtggcg attccgacc ctcttcggc acgactttg gagaagatc aatttggct gtgatggct ataattagt ggcgaaacg gtcgaggac gggtttaat ttagccaca aagatcatt gaggacttt aaattaaga ccgatcaag gtttattgt ttagctggg aaacagttt gcgaaatct gagcgctat aacggaata acgcagtta gttaactgt atcaaatca acaggaatt aacgattca gctatttcg gatatgttg gatgaaatg ctagcgcac gctgtgggg actttgatc aaggaaaac atcgaaggg accaaagtc gaggacttg atcaaactt attaacgat aaggctacg aagatctct gcatatatt gaagccaaa cagttaaaa acggcgtat ttcctggcc gttaagtac aaacgaacg gctgatatt aggaggatt cagagggag gctgagttg ttaaatatg ccaacaatt aaggccctg tgccagaag gtgctgcaa aactcgcca cacacgcat tccaaagac taa
- >TC033570 RA
cgacaaaat catgatggg catccccgc cagcgggcc ggccctgaa agagtgtcc ctgcgagga agtggacgt ggggtttga atgcagatg cagcggtcc ggccctgat ggacatccc caacagcct gcataaaga gctcaccat tctggttat cgaggacgc cgggattga agtgctgaa aagcgattc gctcgtgcc ctactcgag cacgctgcg agagctata catccaccg cgcccccaa actcctgaa aatccagcc ggaggtttt cagcgctta cctccccaa cctcaagat cttgaggat cgtcaacac gggattaga ggaaatgcc gaacttcaa cggattgaa cacttcctc ggtgttgca catgataga tttggagca taaccgcat ccagagaat caacacgcg gcaaattag cagagtcaa ggctgagca actagtgct caactacaa cgatttgca agtggtcga agaggcggc atttgcggg ctcgcagat cgctaaatt aagcctgag tggaaattt tcgactgaa aaacctcca cgtgaaggc gtttcacgg actggagag cttgcgcta catcgacct ctcaagcac tgccataac ggcgttacc cgtggaagg cctgcggga aatcgacgt cctcaaagt ccaagacac agagtcgct taaagtctt tccctcagt ttacaactt caagtacat aaaagaagc ctggctgac ctaccctta ccattgttg cgccttcaa gttccccca cacccacaa cccctggga gtacgaaag acacaaccg atttgtcaa gcagcagcg ccaagcttg caccgccaa aaacgccaa aaacgctga cccgactct gagggcgct gtttagggc cattccgcc gaatgcaag tctcgctga gagcgattt tgacgagca gtggaacgg cgatgagat ttttcacaa cagtacgat gaatctgaa taatggatc gccggttac taccgccgt ttgtggaga gatatacag gaattatca cgaggtcaa gtgtcatcc agcgccgga cgccttcaa cccctgcga agacctgat gggaaattg ggctttaag gataccggt atggttcat atcgttatc ggcagtgat cggaaatct gttcgtggt aatagtcat ctcgacatc gcattttcg actgacagt ttcgaaatt tctcatgtg caatttagc cgtggctga tttatgcat cggcatcca tctgctgct tattgccgt ggtggacgc ctgctccat aagggccta tttcaatta cgccatcga ttggcaaga aggcaacgg ctgtcgcgt tgccggcac gctcaccat cttcggcaa cgtcctctc ggtgtacac cctcgctgt gataaccac cgagcgatg gtacaccat cacctgggc catccatct caacaagcg cctaaagct ccggacggc agttcgcgt gatgatcgc cggctggat ttgtgccct ttttatggc atgtctccc ccttgtggg ggtgagcag ctactccaa aacgagcat atgtctgcc cttggagaa caaaggcaa agccgacac gatatatct ctccacttt gctcgcttt caacgcgat cgcgttcgc cctcatctg cgtctgcta cggaagtat gtacaagtc gattcgtga gggtggaca cacgggatc tacgacttc cgtccggtc cgacctcac tgtggccaa gaggatggc tttgctcgt cttcaccga ttttgcgtg ctgggcgcc gatcgcctt cttcagcct cacggcgct tttaggcca tccgttgat caccgtcac gcagacgaa gatcctcct cgtgttctt ctacccgct caactcgtg cgccaaccc ctgtctgta cgccctgct gacgcagca ataccgccg cgacttttt catcttaat ggggcgcta cggcctgtg caaggaccg cgccgagcg ccacaaggg ggccatcgg gggcaaacc gctcccgta cggctccgg cccgcagta taagcggac tagcggcga ggcagccgg caaaattca cagtgcggg aggtaacaa ccatcgagg gtctctttt gaccactat cgtttcagt agaagtgcc catggtgag gactagttc ccggaacag tgcgcgctc gcgcccgga ctcgctcaa cgccgttcc ggagtattc cctccagca aatcaacct cggcatgat ggacgattg ccggaaact cacttcgac cgataactt ctgtttgtc acaacaaag tgacacatg ttatcaaac aaaaccttt tttattatt aatcagtga atgcttttt tccgcaatg tttaatccc gactcgacc tgacatgga tcagttaat tacgagcct tgataagct cctgaaata caaaaacga ggatccctc ccgttttta caccttctt gagcgattg caccgaaac cgatgaaaa acaatggga aagtttgat acctaaaat ttactatct gtatgatag acaagtttt acgcaaaga tctcgttta tctttcatt gttggcgag ccagaatca cgctctttt acggaaatt tgttgatta cgataagtc tatttacgt tttaaatgt tgccgatcc agtctcgct ttacgatta ttatgttta caaggggaa acactgggt ttatgagat aatagacaa tccgggcaa tcatgcgaa acttgttga tttgaaaaa tatttacaa taaattcca aatgataaa atacgttaa agagacaaa cacgtctgt tgttgacct cgaccagct tgagcgtct ctcccacgc aataaattt cgagttttt ggacgattt ttggaaatt ttacgtcgc cgagataac gcgatttat cacaacagt aaagttttg taaaactct caaaccgga tgcaaacga ttcagattt atttgtttt cgcgagtaa aaacacaca acttgatac tttatcgcg tttcaccga cttgagcga tgccacaca atgggaaaa catcttagc taaactatg caataattg cgaccaatt taacatttt caccggctt tgtgatact cgcgacttt gatcaatct cctaataga caattacaa caccaattt gaaaaaaga tcacgtcaa tgccctctt cgaaaccgt caaaaccaa actcatcga cttaaaaga caagcaatt acagatcga attactcga gaatatttt ccgcttgat gtttcaccg aaagtcgca cgaattcac ttgcaaaga gaaagaaat ccgcttcgt tttgttttt actaaagtc agtaacaga ggaattaaa attgaaaaa agtctacga acgcgactc cgaacacta tcgaagact ctcaactct ctcgattta cgtaacgga cgccttgtg gcgtctcga cttgatcct caacacaaa aaacacccc aaaaataca agaacaact cctcaacta catgctcgc acctccgga gtccctcat ccacatctg cctcaaacg gcacaactt cgaacgcgc ccaccaggt gatagagat tttcgatct gagggactc tgatctcgc gcaacaagt caactactc ggaaacttt cctcaaatt caggaaatt cctccgaaa aaacggcaa attggcgca aacagtcac cgaattttt cgaatcaaa ttcgccaaa cgaaatttt tacgaatat tttcgatct tgcaatcac cggaaccca aacgtggga cgaatcaga aattttgct aaacctagc cgaaaaatt caattccga cgaaacgct cccttctag ttatctcaa tttctacaa acgagtgac ggaaattgg gcacgaagt gggccccaa agacaacat ttcactgcc ccagattct aatttcccc aaactatcc cttggatgt taacgtcta caaaaccaa agtggaatt tttccaaaa cttggcccg actttatga cgaatttaa cggtcaaaa ccgccctaa tgaacccgg gagtttcaa taaaaacca cccaaatca caaaacctt gctcaaaat gaacgaact gatcgttaa ttacctcca agatcggga cacaacgca atacctcgc ccgcatttt caattactt gaaagcctt ttcgaaaat tttgtacat cgagcagaa cagttcgga catcatttc gaagggaaa aaactcgtc ttacttcga cattttcaa cttcaaccg ctcggaatt gaccgggaa attgctgtt cgagcgtca gctggaccc gactgagtt tgaaaaata cttcgggaa actcaagtt ggatttttt gtaccacgt ggtggggaa ttgcttccc gacgattaa tctccacgt gcaggaaaa tgtgaccat tgaagagct gtaccatga taacatgtt gtacattcc gagccgcag cataattgt gtacatcca gaaacgcaa ctggttgct tgcctttat cctgaacga gatgtacaa agttgaagg agtcaaaat cgatattag cgaagttcg cttaaggac gttctttaa ttatttaaa atcgcccag aatccagca cttgaaggc gcttttcga caaaaacga catcatcgc agcgctcca gaactgcat aaatgccca aaaagtcaa gttatattt ccgcgaacg cattatcga gcatgatct agtctcgat ccagtcgca aaacagcag caattctga cggttttga aaccgggga agagattca agaagacgc aaaaacgac caactggag aaccctcta cgatattat cgaaagcat tccggaaaa tcagatgaa gaaaaagtc agacttgat catttgcaa ggacaatgt tctggttaa catggtgca agacgggtt cgagcccga atattacaa atacgctca ttttattac aaacaggga cttgcggat tgacacgat tttgagcaa ttttaggaa atggccggg gaaattcgc cgctgatat aatcaaaag tgagattag tagattcga ttgtttgat ggattcgaa aatggagac gttgtgtgg ttggttgca acaaattga gctcaacga agagctgaa aagcgtttt tgaggtgaa atcgtggta caagagcta cgctttttg caaggaggt gcccgaaag cgcccttga aaaaatcct gctaacgaa taatgtgga ctttttgct ggacttcgt ccagttgta ccaaccctc gcccgaaac cgtctgcaa aatcaattc gagttactt aacccgaat aatggacac gaacattcc actcgaccg gataaaaaa actgctcga tagtctacc cttcgagca cgccataga catgtgcca taacttact cacaatctt acacaactt ggaatatct acaattcat cgccgatta tctaagcac caacgtttc ggacgactt attcctaaa gaacgtcca aatcagttt gaaaatgct ctcggtctt cacaccact cgagcaaga ccaactttt ctgtctcat aaacgaacc tttgagcat cctcgaagt gctcctaat gaacacaaa actggacaa actcgcggc tattttaga cttgctaaa cgcccacaa tttaagcga ctttagcga ggttataac cacagagaa aatcgacga attgttgcg gaaatacgc cgagaagag cttggattt tcgcgttat cacgcaccc taatcccgg cttatttaa aacaccgga gccgaaatt attacaatc gttggactc gcttagttt aatgtccga taaacgcca gtttgtcgt ccctgaaaa cgtcccaac taaggaaga gtgggttca aaacgacga agtcgtcga gtgcatgtg ttgccacca ggtcacttt ttccatgtt caatcggcg gcaccactg tcggagatg tggccgcgt tgtttgtta caattgttc cttgaaacg catgttcgt gcccactta tggcgacat tctagtgag agtttgctc cgactgtta taatcagac agtcgggga gagaaacga cagcaattc ggacaaaag ccgctcctt tatacagga atattggct cttgaacga cgaccccga acataataa aatcataag agaggagtt ctcctacga acacgcccc tagcacctc tctgtgttt ctcaatcct gagatatca ctccaaaag tatcgaata tcccaagtt tttattaga gcagtgtga agtcatgtt gaagttatt gcaaccgaa tcaagaggc ggcccaaga aattgacta tctcctcgt tataaaaat gttaaagtc actcgctat ggcggccaa gatggtctc gactgaaaa tatgctcca atcgggtgc ttccatggc cgaccggat tctctcaca ggccgaact tttggccct tttggcaga acgcggctg tttgaatct tatgccgag tcaaaatct ttacgtcga tgctaatgt tttgcgccg attgcgaga taaattgct acagaggga gcagtggaa tttggccct ggaggtgtc caccaaagc gggactcga caataccgg ggtttttgc cgcctggag caagagctg ccttaaggc gggcagcct gctcctagc ccgcgaaaa gttccaacg ctgccttga caagaacag ccaatacga aacctcgtc aaatttgag ccgactttc cgagctcaa ttcgagttt agatagttt gagtttgag tcgatctcg gatgagttc acgattctc aagcggaag tttcacctt agacaataa accagctaa agatccgcc cctcttgta cgaaatcat ccaaatttt ggaaaccaa aacgcggac tataaaccc gaaagttgt cgaaaaagc ccaaagtat caagatttc aggctcggt cacgagcct caaccaaat atcatcatc gtacaccgt gcacattga cacagcctt aagcatcgt aaaccgggt caaaaattt gaaagaaat cgcatcggg gaattactc actgaccgc aagcaaaaa attcaattg ttacactag tcggcccac aatcgaccc gatttttta cgaggaatg tatctatta tttgggcaa atacgggac gcatttatc gctactgga gttttatat caggcatgg ggaaatcca cactgccct catatacat actcgagtg ccatctacc gagcgaaac cttcatcga agtctatat gaagtgcct taaagacgg gatcgtaac aattttgca agagcacat gtcacggat cgactcatc gctcgaact gtggaaaga ccacttgaa gaatttgtg tcgccactt ggagaagca acaattatt gaactgttt gtaccagtt gcagctgta catcggcga ctatgtgcg cgcttcgat gacttgtat ccgattcta ccaagacaa cgtgaaaag tttcacaga acttgcgaa caatgtcgg ctttttgca caagggcga ggggcattt gaggcagat tttggagca ggagcagtg ggtggaggt gacttcggt cacttccaa ggatagttt cgaggagag actcttgac caattcgga cttggtcat gaaaatcga gtcgaaaga cgtcaaccg acacataaa tacagtaaa acgacaggt ggaaattgt gaaattttt ggccgattg tgaggcggc aggggtcaa cacgcgcga cctgcttcg gcagctggt tttgaccaa agatcctaa agccgagca gcaagtggc gattccgac cctcttcgg cacgacttt ggagaagat caatttggc tgtgatggc tataattag tggcgaaac ggtcgagga cgggtttaa tttagccac aaagatcat tgaggactt taaattaag accgatcaa ggtttattg tttagctgg gaaacagtt tgcgaaatc tgagcgcta taacggaat aacgcagtt agttaactg tatcaaatc aacaggaat taacgattc agctatttc ggatatgtt ggatgaaat gctagcgca cgctgtggg gactttgat caaggaaaa catcgaagg gaccaaagt cgaggactt gatcaaact tattaacga taaggctac gaagatctc tgcatatat tgaagccaa acagttaaa aacggcgta tttcctggc cgttaagta caaacgaac ggctgatat taggaggat tcagaggga ggctgagtt gttaaatat gccaacaat taaggccct gtgccagaa ggtgctgca aaactcgcc acacacgca ttccaaaga ctaacctgt aaaataaac cgggctgtg atgt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
