| General information | |||
| TC009445 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1149154..1152524 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009445 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06272 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC009445 RA
MQAKTDLVI GIDLGTTNS SAAYYFKEK VRVVENKEG DRITPSCVY FRDQNTVIV GKYARKMAE QSNQSEVFG IKRFIGKQF DDPDLRNDL RHVPFTIES IENKPIVTI NHKSGVCKK TPEEVSALV LQKVKTDVE SKLGERVNK AVITVPAYF NVSQREATL EAAQKAGFT VLKLLNEPT AAAFCYYVD QNWGEESYS LVYDLGGGT FDVAILKNC RQNIDIVGV DGDTHLGGH DFDNLIIDY VCDILLKEY DYNPKDDRR NMRRLRSIC EEAKQTLSD LEETIIILP AFTKKHDII NINITREQF ESMAQMLFQ RTIDIVDKC LTTCNIAKT EIKEVILSG GSTRIPEIQ NLLSSYFGG KELCKFTHP GECVAEGAA IQAAILSTN PDQKINTIQ IKDVISLSL GIDVHFNLM FFIIKRNRS IPIKKTKSL ITIFNQQSA MSINIYEGE RTDVRKNRH LGTLKITNL TPAPPGQCE VHVIMSVDQ NGILTFRAK EKFRNNEKD LKLLYTRGG RSDSEVKSI LQKVEDQAE EDERFEKFA MKKTVLFNY CETVIYNLE SKNLSSSYK EVYDLCKDT QNKLESLEL GSEDQVNPL VEAAKSKCD SLVRQYNFD YMFDL
- >TC009445 RA
atgcaggca aaaaccgat ttagtcatt ggtatcgat ctgggaaca acaaattcg tctgccgcc tattatttt aaagaaaaa gtcagagtt gtagaaaat aaagaaggt gatcgaatt accccatct tgtgtgtat tttcgtgac caaaatacg gtaattgtt ggaaaatat gcccgaaaa atggctgag caatcaaat caaagtgaa gtttttgga attaagcga tttattgga aaacagttt gatgatcct gatcttcga aacgattta agacacgtt ccattcaca attgaatca attgagaac aaaccgata gtcacgatt aatcataaa agtggtgtt tgtaaaaag actcctgaa gaagtaagt gcacttgtt cttcagaaa gttaagaca gacgtcgaa tcgaaacta ggagaacgt gtaaataaa gctgtaatt actgttccg gcttacttt aacgtaagt caacgtgag gctacttta gaagcggca caaaaagct ggatttact gtattaaaa cttctaaat gaacctaca gctgctgcg ttctgttac tacgtggac caaaattgg ggtgaagaa agttattcg ttggtttat gatttagga ggtgggact ttcgacgtt gccattctt aagaactgt cgtcaaaat attgatatt gttggagtt gatggtgat acacattta gggggccac gacttcgac aatttgatt atagattac gtttgtgat attttattg aaagaatat gattacaat cccaaggat gacagaaga aacatgaga agattacgt agcatatgt gaagaggcc aaacaaaca ttatcagat ctggaagag actattata atactcccg gcatttaca aaaaaacat gatattatt aacattaac atcacacga gaacagttt gaatcaatg gcccaaatg ttgtttcaa agaaccata gacattgtc gataagtgt ttaacaact tgcaacatt gcaaagaca gagataaag gaagtaatc ctatcaggc ggttctaca cgaattcca gaaatacaa aatttactt tcgtcatat ttcggtgga aaagaatta tgcaagttt acacatcct ggcgaatgc gtagctgaa ggagcggcg attcaagca gctattctg tccaccaat ccagatcaa aagataaat actattcaa ataaaagat gttatttct ttgtctctt ggtatagat gttcatttc aatttaatg tttttcatt ataaagcga aacagatca attcctata aaaaagaca aaaagtcta ataactatt ttcaatcaa caatccgca atgtctata aacatatac gaaggagaa cgcaccgat gttagaaaa aaccgtcac ttgggcact ctaaaaatt accaactta acaccagca ccacctgga caatgcgaa gttcacgtg atcatgagc gtcgatcaa aatggcatc cttacattt agagcaaaa gaaaaattt cgaaataac gaaaaggat ctaaaatta ttatacaca cgaggcggc cgaagtgat agtgaagta aaaagcatt ttacaaaaa gttgaagac caagccgaa gaagacgaa cgatttgaa aaatttgcg atgaagaaa acagttctt ttcaattac tgtgaaaca gtaatatac aatctagaa agcaagaac ttaagctcg tcgtacaaa gaagtttat gatctttgt aaagacaca caaaataaa ttagaatca ctggaacta ggaagtgaa gaccaagta aatccactc gtcgaagct gctaaatca aaatgcgat tcacttgtg cggcagtat aattttgat tacatgttt gatttgtaa
- >TC009445 RA
gcagaagtg aaagagaag ataaaacta agttagagc aaacgaagc agttttagc agttttgtt tgtctttta cacaaacat gcaggcaaa aaccgattt agtcattgg tatcgatct gggaacaac aaattcgtc tgccgccta ttattttaa agaaaaagt cagagttgt agaaaataa agaaggtga tcgaattac cccatcttg tgtgtattt tcgtgacca aaatacggt aattgttgg aaaatatgc ccgaaaaat ggctgagca atcaaatca aagtgaagt ttttggaat taagcgatt tattggaaa acagtttga tgatcctga tcttcgaaa cgatttaag acacgttcc attcacaat tgaatcaat tgagaacaa accgatagt cacgattaa tcataaaag tggtgtttg taaaaagac tcctgaaga agtaagtgc acttgttct tcagaaagt taagacaga cgtcgaatc gaaactagg agaacgtgt aaataaagc tgtaattac tgttccggc ttactttaa cgtaagtca acgtgaggc tactttaga agcggcaca aaaagctgg atttactgt attaaaact tctaaatga acctacagc tgctgcgtt ctgttacta cgtggacca aaattgggg tgaagaaag ttattcgtt ggtttatga tttaggagg tgggacttt cgacgttgc cattcttaa gaactgtcg tcaaaatat tgatattgt tggagttga tggtgatac acatttagg gggccacga cttcgacaa tttgattat agattacgt ttgtgatat tttattgaa agaatatga ttacaatcc caaggatga cagaagaaa catgagaag attacgtag catatgtga agaggccaa acaaacatt atcagatct ggaagagac tattataat actcccggc atttacaaa aaaacatga tattattaa cattaacat cacacgaga acagtttga atcaatggc ccaaatgtt gtttcaaag aaccataga cattgtcga taagtgttt aacaacttg caacattgc aaagacaga gataaagga agtaatcct atcaggcgg ttctacacg aattccaga aatacaaaa tttactttc gtcatattt cggtggaaa agaattatg caagtttac acatcctgg cgaatgcgt agctgaagg agcggcgat tcaagcagc tattctgtc caccaatcc agatcaaaa gataaatac tattcaaat aaaagatgt tatttcttt gtctcttgg tatagatgt tcatttcaa tttaatgtt tttcattat aaagcgaaa cagatcaat tcctataaa aaagacaaa aagtctaat aactatttt caatcaaca atccgcaat gtctataaa catatacga aggagaacg caccgatgt tagaaaaaa ccgtcactt gggcactct aaaaattac caacttaac accagcacc acctggaca atgcgaagt tcacgtgat catgagcgt cgatcaaaa tggcatcct tacatttag agcaaaaga aaaatttcg aaataacga aaaggatct aaaattatt atacacacg aggcggccg aagtgatag tgaagtaaa aagcatttt acaaaaagt tgaagacca agccgaaga agacgaacg atttgaaaa atttgcgat gaagaaaac agttctttt caattactg tgaaacagt aatatacaa tctagaaag caagaactt aagctcgtc gtacaaaga agtttatga tctttgtaa agacacaca aaataaatt agaatcact ggaactagg aagtgaaga ccaagtaaa tccactcgt cgaagctgc taaatcaaa atgcgattc acttgtgcg gcagtataa ttttgatta catgtttga tttgtaatt tttaacata gcagacaaa tgtatgatc aacgaacca taattttta cgtttgaca gaacagaga gaattttaa gttttagta attatcaac tgtagtttt tagggcgac ttttctttg atttgttaa aaaatactg ggaaaataa aaaaataat ttttca
