Status
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Gene information
General information
TC009445 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1149154..1152524
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009445
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06272

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009445 RA
      MQAKTDLVI GIDLGTTNS SAAYYFKEK VRVVENKEG 
      DRITPSCVY FRDQNTVIV GKYARKMAE QSNQSEVFG 
      IKRFIGKQF DDPDLRNDL RHVPFTIES IENKPIVTI 
      NHKSGVCKK TPEEVSALV LQKVKTDVE SKLGERVNK 
      AVITVPAYF NVSQREATL EAAQKAGFT VLKLLNEPT 
      AAAFCYYVD QNWGEESYS LVYDLGGGT FDVAILKNC 
      RQNIDIVGV DGDTHLGGH DFDNLIIDY VCDILLKEY 
      DYNPKDDRR NMRRLRSIC EEAKQTLSD LEETIIILP 
      AFTKKHDII NINITREQF ESMAQMLFQ RTIDIVDKC 
      LTTCNIAKT EIKEVILSG GSTRIPEIQ NLLSSYFGG 
      KELCKFTHP GECVAEGAA IQAAILSTN PDQKINTIQ 
      IKDVISLSL GIDVHFNLM FFIIKRNRS IPIKKTKSL 
      ITIFNQQSA MSINIYEGE RTDVRKNRH LGTLKITNL 
      TPAPPGQCE VHVIMSVDQ NGILTFRAK EKFRNNEKD 
      LKLLYTRGG RSDSEVKSI LQKVEDQAE EDERFEKFA 
      MKKTVLFNY CETVIYNLE SKNLSSSYK EVYDLCKDT 
      QNKLESLEL GSEDQVNPL VEAAKSKCD SLVRQYNFD 
      YMFDL
    CDS sequence
    • >TC009445 RA
      atgcaggca aaaaccgat ttagtcatt ggtatcgat 
      ctgggaaca acaaattcg tctgccgcc tattatttt 
      aaagaaaaa gtcagagtt gtagaaaat aaagaaggt 
      gatcgaatt accccatct tgtgtgtat tttcgtgac 
      caaaatacg gtaattgtt ggaaaatat gcccgaaaa 
      atggctgag caatcaaat caaagtgaa gtttttgga 
      attaagcga tttattgga aaacagttt gatgatcct 
      gatcttcga aacgattta agacacgtt ccattcaca 
      attgaatca attgagaac aaaccgata gtcacgatt 
      aatcataaa agtggtgtt tgtaaaaag actcctgaa 
      gaagtaagt gcacttgtt cttcagaaa gttaagaca 
      gacgtcgaa tcgaaacta ggagaacgt gtaaataaa 
      gctgtaatt actgttccg gcttacttt aacgtaagt 
      caacgtgag gctacttta gaagcggca caaaaagct 
      ggatttact gtattaaaa cttctaaat gaacctaca 
      gctgctgcg ttctgttac tacgtggac caaaattgg 
      ggtgaagaa agttattcg ttggtttat gatttagga 
      ggtgggact ttcgacgtt gccattctt aagaactgt 
      cgtcaaaat attgatatt gttggagtt gatggtgat 
      acacattta gggggccac gacttcgac aatttgatt 
      atagattac gtttgtgat attttattg aaagaatat 
      gattacaat cccaaggat gacagaaga aacatgaga 
      agattacgt agcatatgt gaagaggcc aaacaaaca 
      ttatcagat ctggaagag actattata atactcccg 
      gcatttaca aaaaaacat gatattatt aacattaac 
      atcacacga gaacagttt gaatcaatg gcccaaatg 
      ttgtttcaa agaaccata gacattgtc gataagtgt 
      ttaacaact tgcaacatt gcaaagaca gagataaag 
      gaagtaatc ctatcaggc ggttctaca cgaattcca 
      gaaatacaa aatttactt tcgtcatat ttcggtgga 
      aaagaatta tgcaagttt acacatcct ggcgaatgc 
      gtagctgaa ggagcggcg attcaagca gctattctg 
      tccaccaat ccagatcaa aagataaat actattcaa 
      ataaaagat gttatttct ttgtctctt ggtatagat 
      gttcatttc aatttaatg tttttcatt ataaagcga 
      aacagatca attcctata aaaaagaca aaaagtcta 
      ataactatt ttcaatcaa caatccgca atgtctata 
      aacatatac gaaggagaa cgcaccgat gttagaaaa 
      aaccgtcac ttgggcact ctaaaaatt accaactta 
      acaccagca ccacctgga caatgcgaa gttcacgtg 
      atcatgagc gtcgatcaa aatggcatc cttacattt 
      agagcaaaa gaaaaattt cgaaataac gaaaaggat 
      ctaaaatta ttatacaca cgaggcggc cgaagtgat 
      agtgaagta aaaagcatt ttacaaaaa gttgaagac 
      caagccgaa gaagacgaa cgatttgaa aaatttgcg 
      atgaagaaa acagttctt ttcaattac tgtgaaaca 
      gtaatatac aatctagaa agcaagaac ttaagctcg 
      tcgtacaaa gaagtttat gatctttgt aaagacaca 
      caaaataaa ttagaatca ctggaacta ggaagtgaa 
      gaccaagta aatccactc gtcgaagct gctaaatca 
      aaatgcgat tcacttgtg cggcagtat aattttgat 
      tacatgttt gatttgtaa
    mRNA sequence
    • >TC009445 RA
      gcagaagtg aaagagaag ataaaacta agttagagc 
      aaacgaagc agttttagc agttttgtt tgtctttta 
      cacaaacat gcaggcaaa aaccgattt agtcattgg 
      tatcgatct gggaacaac aaattcgtc tgccgccta 
      ttattttaa agaaaaagt cagagttgt agaaaataa 
      agaaggtga tcgaattac cccatcttg tgtgtattt 
      tcgtgacca aaatacggt aattgttgg aaaatatgc 
      ccgaaaaat ggctgagca atcaaatca aagtgaagt 
      ttttggaat taagcgatt tattggaaa acagtttga 
      tgatcctga tcttcgaaa cgatttaag acacgttcc 
      attcacaat tgaatcaat tgagaacaa accgatagt 
      cacgattaa tcataaaag tggtgtttg taaaaagac 
      tcctgaaga agtaagtgc acttgttct tcagaaagt 
      taagacaga cgtcgaatc gaaactagg agaacgtgt 
      aaataaagc tgtaattac tgttccggc ttactttaa 
      cgtaagtca acgtgaggc tactttaga agcggcaca 
      aaaagctgg atttactgt attaaaact tctaaatga 
      acctacagc tgctgcgtt ctgttacta cgtggacca 
      aaattgggg tgaagaaag ttattcgtt ggtttatga 
      tttaggagg tgggacttt cgacgttgc cattcttaa 
      gaactgtcg tcaaaatat tgatattgt tggagttga 
      tggtgatac acatttagg gggccacga cttcgacaa 
      tttgattat agattacgt ttgtgatat tttattgaa 
      agaatatga ttacaatcc caaggatga cagaagaaa 
      catgagaag attacgtag catatgtga agaggccaa 
      acaaacatt atcagatct ggaagagac tattataat 
      actcccggc atttacaaa aaaacatga tattattaa 
      cattaacat cacacgaga acagtttga atcaatggc 
      ccaaatgtt gtttcaaag aaccataga cattgtcga 
      taagtgttt aacaacttg caacattgc aaagacaga 
      gataaagga agtaatcct atcaggcgg ttctacacg 
      aattccaga aatacaaaa tttactttc gtcatattt 
      cggtggaaa agaattatg caagtttac acatcctgg 
      cgaatgcgt agctgaagg agcggcgat tcaagcagc 
      tattctgtc caccaatcc agatcaaaa gataaatac 
      tattcaaat aaaagatgt tatttcttt gtctcttgg 
      tatagatgt tcatttcaa tttaatgtt tttcattat 
      aaagcgaaa cagatcaat tcctataaa aaagacaaa 
      aagtctaat aactatttt caatcaaca atccgcaat 
      gtctataaa catatacga aggagaacg caccgatgt 
      tagaaaaaa ccgtcactt gggcactct aaaaattac 
      caacttaac accagcacc acctggaca atgcgaagt 
      tcacgtgat catgagcgt cgatcaaaa tggcatcct 
      tacatttag agcaaaaga aaaatttcg aaataacga 
      aaaggatct aaaattatt atacacacg aggcggccg 
      aagtgatag tgaagtaaa aagcatttt acaaaaagt 
      tgaagacca agccgaaga agacgaacg atttgaaaa 
      atttgcgat gaagaaaac agttctttt caattactg 
      tgaaacagt aatatacaa tctagaaag caagaactt 
      aagctcgtc gtacaaaga agtttatga tctttgtaa 
      agacacaca aaataaatt agaatcact ggaactagg 
      aagtgaaga ccaagtaaa tccactcgt cgaagctgc 
      taaatcaaa atgcgattc acttgtgcg gcagtataa 
      ttttgatta catgtttga tttgtaatt tttaacata 
      gcagacaaa tgtatgatc aacgaacca taattttta 
      cgtttgaca gaacagaga gaattttaa gttttagta 
      attatcaac tgtagtttt tagggcgac ttttctttg 
      atttgttaa aaaatactg ggaaaataa aaaaataat 
      ttttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY