Status
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Gene information
General information
TC009310 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 224562..228895
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009310
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06268

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head appendages partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009310 RA
      METLCEHVD ADDENFLLD DENAIKSCN KCKSPSNGF 
      VTMDDHVDT LEDSPLLIN DCYHDEFHC HNILQQSED 
      KKAWKKLLI AAILCFFFM ITELIGGFI AGSLAVMTD 
      AAHLFSDFI GFLISLLSI WVARKAPTR NMTFGYYRA 
      EVLGAFLSV LTVWLLAGV FAVLAINRL LKKEYDIEA 
      NTMMLVASL GLVVNIVMG AVLFGFCHN HSHGLSDQS 
      NSNINVRAA AAHVLGDLL QSLGVLIAS IIIKVFPSA 
      KVADPICTL IFSAVVIFT TAKVAKDSI WLLLEGSPK 
      HSGDLAFEL LNISNVRHL HNLHIWALS PGKDAVSVH 
      LCVDKYSNR DSILKKANS LIKSRLNVI SCTIQIETY 
      SLELINSYK ELRVRVDPL KIVY
    • >TC009310 RB
      METLCEHVD ADDENFLLD DENAIKSCN KCKSPSNGF 
      VTMDDHVDT LEDSPLLIN DCYHDEFHC HNILQQSED 
      KKAWKKLLI AAILCFFFM ITELIGGFI AGSLAVMTD 
      AAHLFSDFI GFLISLLSI WVARKAPTR NMTFGYYRA 
      EVLGAFLSV LTVWLLAGV FAVLAINRL LKKEYDIEA 
      NTMMLVASL GLVVNIVMG AVLFGFCHN HSHGLSDQS 
      NSNINVRAA AAHVLGDLL QSLGVLIAS IIIKVFPSA 
      KVADPICTL IFSAVVIFT TAKVAKDSI WLLLEGSPK 
      HSGDLAFEL LNISNVRHL HNLHIWALS PGKDAVSVH 
      LCVDKYSNR DSILKKANS LIKSRLNVI SCTIQIETY 
      SLELINSYK FVIFPIYLF ILLQ
    CDS sequence
    • >TC009310 RA
      atggagaca ctgtgtgag catgttgac gccgacgac 
      gaaaatttc ctcctcgat gatgaaaac gctatcaaa 
      agctgtaat aaatgcaaa agtccttcc aatggtttt 
      gtaacaatg gacgaccac gttgacacc cttgaagat 
      tcaccactt ctcatcaat gattgctat catgacgaa 
      ttccattgc cacaacatt ttgcaacaa tccgaagac 
      aaaaaagcc tggaagaaa ctcctaatt gcggcaatt 
      ctgtgcttc ttttttatg attacggaa ctaattggt 
      ggttttatt gccggtagt cttgccgta atgacagat 
      gcggcgcat cttttctca gattttatc gggtttctt 
      atttcgctt ttatcaatt tgggtggca cgtaaagct 
      cccacaaga aatatgact tttgggtat tacagagca 
      gaggttttg ggcgcattt ttgagcgtt ttaaccgtt 
      tggttactc gccggcgtt tttgcagta cttgcaatt 
      aacagattg ttgaagaaa gaatacgat attgaagct 
      aatactatg atgttggtg gcctcatta ggccttgtt 
      gtaaatatt gtgatgggg gctgtgctt ttcggcttt 
      tgtcacaac cacagtcat ggccttagc gaccaaagc 
      aattcgaat attaacgtg cgagctgca gcagctcac 
      gttctgggc gacctttta caaagttta ggggtgctt 
      atagcttca attattatt aaagtgttt cctagtgcc 
      aaagtggcc gacccgatt tgcacttta attttttca 
      gcggttgtt attttcaca actgctaaa gttgcaaaa 
      gattccatt tggttgtta ctggaaggc agtcccaaa 
      cattccggt gatctagca tttgagtta ttaaatatt 
      tcgaatgtt agacatctt cataattta catatctgg 
      gctctatct cctgggaag gatgccgtt tcagtccat 
      ttgtgtgtt gataaatac tctaatcgt gattctatt 
      ttaaagaaa gctaattca ctcattaag agtcgatta 
      aatgtgata agttgtact attcagatt gaaacttat 
      agtttagag ttgattaat tcgtacaaa gagttgcga 
      gttcgtgta gatcctctg aaaatcgtc tactga
    • >TC009310 RB
      atggagaca ctgtgtgag catgttgac gccgacgac 
      gaaaatttc ctcctcgat gatgaaaac gctatcaaa 
      agctgtaat aaatgcaaa agtccttcc aatggtttt 
      gtaacaatg gacgaccac gttgacacc cttgaagat 
      tcaccactt ctcatcaat gattgctat catgacgaa 
      ttccattgc cacaacatt ttgcaacaa tccgaagac 
      aaaaaagcc tggaagaaa ctcctaatt gcggcaatt 
      ctgtgcttc ttttttatg attacggaa ctaattggt 
      ggttttatt gccggtagt cttgccgta atgacagat 
      gcggcgcat cttttctca gattttatc gggtttctt 
      atttcgctt ttatcaatt tgggtggca cgtaaagct 
      cccacaaga aatatgact tttgggtat tacagagca 
      gaggttttg ggcgcattt ttgagcgtt ttaaccgtt 
      tggttactc gccggcgtt tttgcagta cttgcaatt 
      aacagattg ttgaagaaa gaatacgat attgaagct 
      aatactatg atgttggtg gcctcatta ggccttgtt 
      gtaaatatt gtgatgggg gctgtgctt ttcggcttt 
      tgtcacaac cacagtcat ggccttagc gaccaaagc 
      aattcgaat attaacgtg cgagctgca gcagctcac 
      gttctgggc gacctttta caaagttta ggggtgctt 
      atagcttca attattatt aaagtgttt cctagtgcc 
      aaagtggcc gacccgatt tgcacttta attttttca 
      gcggttgtt attttcaca actgctaaa gttgcaaaa 
      gattccatt tggttgtta ctggaaggc agtcccaaa 
      cattccggt gatctagca tttgagtta ttaaatatt 
      tcgaatgtt agacatctt cataattta catatctgg 
      gctctatct cctgggaag gatgccgtt tcagtccat 
      ttgtgtgtt gataaatac tctaatcgt gattctatt 
      ttaaagaaa gctaattca ctcattaag agtcgatta 
      aatgtgata agttgtact attcagatt gaaacttat 
      agtttagag ttgattaat tcgtacaag tttgtaatt 
      tttccaatt tatttattt atattatta caataa
    mRNA sequence
    • >TC009310 RA
      tatgagttg gcaaattaa aattagaat tagaatctc 
      aaccattcc aaaccagat ttgcaattt ctgtttccg 
      gtcgtttca gtccgccat ctgaaacaa cagtaatta 
      ctacacatt cgcacatgt tgaaaattt tcaacgcaa 
      ctaatttca tccaacaat gatgaaaat ttttggcga 
      gttttcggc attcaatca ttttggaga taatgggca 
      gtagagtgg gcgtgtttt ggtcagagg ttgcaaagt 
      acatgtgtt tttgtcaga taatagata gtatatctc 
      tggagggcg gcgacttgc tatttgtca taagcatcc 
      tgccaacat tggtcatat gaattacgg tttcttatt 
      cttgtaatg ttgttatct tgttcggac aggggacgt 
      tatttattg cttcggttt gtgggagtt atgtacatt 
      tttatgaca tatggacca aataaagac cttaccttt 
      tttatgtca cttgccaag aaaacagac ttttttatc 
      gaaacacag ttatatttc aaatacgag tattgttca 
      tatttatta caattcgcg tagttttgt gagtgcaat 
      gatggtacg aagggacaa tttctttat ttataactt 
      tataataca ttttttgtg tccatgatc ttaaactaa 
      attcctatt ttaatccca ctgacgtgt cccgattct 
      agttataat atttctata atagacaaa tagtgataa 
      ttcagtctt aaaaatgtt aagtgttaa accaccatg 
      gttggtgta gtttcaata gttgagaaa aatcgcttt 
      atttgatca aatggagac actgtgtga gcatgttga 
      cgccgacga cgaaaattt cctcctcga tgatgaaaa 
      cgctatcaa aagctgtaa taaatgcaa aagtccttc 
      caatggttt tgtaacaat ggacgacca cgttgacac 
      ccttgaaga ttcaccact tctcatcaa tgattgcta 
      tcatgacga attccattg ccacaacat tttgcaaca 
      atccgaaga caaaaaagc ctggaagaa actcctaat 
      tgcggcaat tctgtgctt cttttttat gattacgga 
      actaattgg tggttttat tgccggtag tcttgccgt 
      aatgacaga tgcggcgca tcttttctc agattttat 
      cgggtttct tatttcgct tttatcaat ttgggtggc 
      acgtaaagc tcccacaag aaatatgac ttttgggta 
      ttacagagc agaggtttt gggcgcatt tttgagcgt 
      tttaaccgt ttggttact cgccggcgt ttttgcagt 
      acttgcaat taacagatt gttgaagaa agaatacga 
      tattgaagc taatactat gatgttggt ggcctcatt 
      aggccttgt tgtaaatat tgtgatggg ggctgtgct 
      tttcggctt ttgtcacaa ccacagtca tggccttag 
      cgaccaaag caattcgaa tattaacgt gcgagctgc 
      agcagctca cgttctggg cgacctttt acaaagttt 
      aggggtgct tatagcttc aattattat taaagtgtt 
      tcctagtgc caaagtggc cgacccgat ttgcacttt 
      aattttttc agcggttgt tattttcac aactgctaa 
      agttgcaaa agattccat ttggttgtt actggaagg 
      cagtcccaa acattccgg tgatctagc atttgagtt 
      attaaatat ttcgaatgt tagacatct tcataattt 
      acatatctg ggctctatc tcctgggaa ggatgccgt 
      ttcagtcca tttgtgtgt tgataaata ctctaatcg 
      tgattctat tttaaagaa agctaattc actcattaa 
      gagtcgatt aaatgtgat aagttgtac tattcagat 
      tgaaactta tagtttaga gttgattaa ttcgtacaa 
      agagttgcg agttcgtgt agatcctct gaaaatcgt 
      ctactgacc acattatta cttatatca caatcacct 
      gcgtaaaaa tagtaaatt acaaccaat tatactaaa 
      aatttcaaa accaaccat gaacttcgt tattttcat 
      ctgtaggta cagtagtac agtccaaca gttgtggca 
      cacagcctc tcacttttt ccgtcaaca ttaatgtcg 
      ttgagactc cagcgtatg gttctgcac cgtttgcca 
      actcacaaa ttaacatac ccacactat aaatgtctg 
      atttttgat tatagcctt gaacaaatt taattaaaa 
      caaaactat caaataaaa taaaatacc ctgcaaatt 
      ttgttccaa aagttcggg acttagcca gtttaggtt 
      cctcacagt gcttgctgg aaacgagtg aatttgcct 
      ttgccattt tccatttaa aacaatcgg acaggcgta 
      tcgtaagcc agcaagaga cgctttccc tgtcgcaga 
      aattaaaat gtgaccagc tctgactga ccttgaata 
      atttggctc aatactcgt tcacctaaa gcaacaaat 
      taagcaacc tgtgaataa accccctct tgtgtatgt 
      aatccaacg catcaagga catccttta aaattaaaa 
      taatagcgt gttcaggca gtc
    • >TC009310 RB
      tatgagttg gcaaattaa aattagaat tagaatctc 
      aaccattcc aaaccagat ttgcaattt ctgtttccg 
      gtcgtttca gtccgccat ctgaaacaa cagtaatta 
      ctacacatt cgcacatgt tgaaaattt tcaacgcaa 
      ctaatttca tccaacaat gatgaaaat ttttggcga 
      gttttcggc attcaatca ttttggaga taatgggca 
      gtagagtgg gcgtgtttt ggtcagagg ttgcaaagt 
      acatgtgtt tttgtcaga taatagata gtatatctc 
      tggagggcg gcgacttgc tatttgtca taagcatcc 
      tgccaacat tggtcatat gaattacgg tttcttatt 
      cttgtaatg ttgttatct tgttcggac aggggacgt 
      tatttattg cttcggttt gtgggagtt atgtacatt 
      tttatgaca tatggacca aataaagac cttaccttt 
      tttatgtca cttgccaag aaaacagac ttttttatc 
      gaaacacag ttatatttc aaatacgag tattgttca 
      tatttatta caattcgcg tagttttgt gagtgcaat 
      gatggtacg aagggacaa tttctttat ttataactt 
      tataataca ttttttgtg tccatgatc ttaaactaa 
      attcctatt ttaatccca ctgacgtgt cccgattct 
      agttataat atttctata atagacaaa tagtgataa 
      ttcagtctt aaaaatgtt aagtgttaa accaccatg 
      gttggtgta gtttcaata gttgagaaa aatcgcttt 
      atttgatca aatggagac actgtgtga gcatgttga 
      cgccgacga cgaaaattt cctcctcga tgatgaaaa 
      cgctatcaa aagctgtaa taaatgcaa aagtccttc 
      caatggttt tgtaacaat ggacgacca cgttgacac 
      ccttgaaga ttcaccact tctcatcaa tgattgcta 
      tcatgacga attccattg ccacaacat tttgcaaca 
      atccgaaga caaaaaagc ctggaagaa actcctaat 
      tgcggcaat tctgtgctt cttttttat gattacgga 
      actaattgg tggttttat tgccggtag tcttgccgt 
      aatgacaga tgcggcgca tcttttctc agattttat 
      cgggtttct tatttcgct tttatcaat ttgggtggc 
      acgtaaagc tcccacaag aaatatgac ttttgggta 
      ttacagagc agaggtttt gggcgcatt tttgagcgt 
      tttaaccgt ttggttact cgccggcgt ttttgcagt 
      acttgcaat taacagatt gttgaagaa agaatacga 
      tattgaagc taatactat gatgttggt ggcctcatt 
      aggccttgt tgtaaatat tgtgatggg ggctgtgct 
      tttcggctt ttgtcacaa ccacagtca tggccttag 
      cgaccaaag caattcgaa tattaacgt gcgagctgc 
      agcagctca cgttctggg cgacctttt acaaagttt 
      aggggtgct tatagcttc aattattat taaagtgtt 
      tcctagtgc caaagtggc cgacccgat ttgcacttt 
      aattttttc agcggttgt tattttcac aactgctaa 
      agttgcaaa agattccat ttggttgtt actggaagg 
      cagtcccaa acattccgg tgatctagc atttgagtt 
      attaaatat ttcgaatgt tagacatct tcataattt 
      acatatctg ggctctatc tcctgggaa ggatgccgt 
      ttcagtcca tttgtgtgt tgataaata ctctaatcg 
      tgattctat tttaaagaa agctaattc actcattaa 
      gagtcgatt aaatgtgat aagttgtac tattcagat 
      tgaaactta tagtttaga gttgattaa ttcgtacaa 
      gtttgtaat ttttccaat ttatttatt tatattatt 
      acaataagt gtcattgga agagcaatc actgtccaa 
      atagctgtt tttataata ctgaaataa agtgattga 
      ctgaattgt ctttttaaa aatcccatt cgcacgaat 
      ataactcca agtcggcga attgttgca caacgtcca 
      aatttgcaa cttcgtcta taaaaacca aaataagaa 
      cttttcaga cacattttg atggcccgg agcagccac 
      ccaagtcac acacgttta cggaaaact gattcttac 
      ggaaaattt tttgatgaa ttcgaatct gtagatggt 
      attgcattc attatgaaa aatttagaa aaaacaata 
      tttgacaaa ttttgtgtt tacattgtc atgtaaaaa 
      aattcagtt ttcttgaaa cgtttcata tttgtgtag 
      tgaaatcct taccaagtt attgcagtt tcaagttgg 
      tgcaataac caaagctcg cacggtaaa ataccccgc 
      agcgatttt cccgttagc agttcattt tttctatga 
      caaatattt caaaggcca aaaaacgct aaataatca 
      aaaaacaat tataattga aacctcaaa gtacagttt 
      atttactaa ctaaatgct tgaaattgg ttaaaattc 
      aacactttt cttgaatca gttttcagt aatcgagtg 
      cggacgaat ttcgtaata agcgtcttt taatcaagc 
      gcctgctcc cggactaat tacagttac ctgtattaa 
      cagctacgc gactactaa gcggcataa caggcttaa 
      gtaaatcag gcaaaaaga ttcctttct ttattaatt 
      taaagatag ggtgtgtga ggagttgac tagcggtgg 
      gacggataa ttgcctcct tttgtagac aaagagttg 
      cgagttcgt gtagatcct ctgaaaatc gtctactga 
      ccacattat tacttatat cacaatcac ctgcgtaaa 
      aatagtaaa ttacaacca attatacta aaaatttca 
      aaaccaacc atgaacttc gttattttc atctgtagg 
      tacagtagt acagtccaa cagttgtgg cacacagcc 
      tctcacttt ttccgtcaa cattaatgt cgttgagac 
      tccagcgta tggttctgc accgtttgc caactcaca 
      aattaacat acccacact ataaatgtc tgatttttg 
      attatagcc ttgaacaaa tttaattaa aacaaaact 
      atcaaataa aataaaata ccctgcaaa ttttgttcc 
      aaaagttcg ggacttagc cagtttagg ttcctcaca 
      gtgcttgct ggaaacgag tgaatttgc ctttgccat 
      tttccattt aaaacaatc ggacaggcg tatcgtaag 
      ccagcaaga gacgctttc cctgtcgca gaaattaaa 
      atgtgacca gctctgact gaccttgaa taatttggc 
      tcaatactc gttcaccta aagcaacaa attaagcaa 
      cctgtgaat aaaccccct cttgtgtat gtaatccaa 
      cgcatcaag gacatcctt taaaattaa aataatagc 
      gtgttcagg cagtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY