| General information | |||
| TC009310 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 224562..228895 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009310 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06268 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
head appendages partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC009310 RA
METLCEHVD ADDENFLLD DENAIKSCN KCKSPSNGF VTMDDHVDT LEDSPLLIN DCYHDEFHC HNILQQSED KKAWKKLLI AAILCFFFM ITELIGGFI AGSLAVMTD AAHLFSDFI GFLISLLSI WVARKAPTR NMTFGYYRA EVLGAFLSV LTVWLLAGV FAVLAINRL LKKEYDIEA NTMMLVASL GLVVNIVMG AVLFGFCHN HSHGLSDQS NSNINVRAA AAHVLGDLL QSLGVLIAS IIIKVFPSA KVADPICTL IFSAVVIFT TAKVAKDSI WLLLEGSPK HSGDLAFEL LNISNVRHL HNLHIWALS PGKDAVSVH LCVDKYSNR DSILKKANS LIKSRLNVI SCTIQIETY SLELINSYK ELRVRVDPL KIVY
- >TC009310 RB
METLCEHVD ADDENFLLD DENAIKSCN KCKSPSNGF VTMDDHVDT LEDSPLLIN DCYHDEFHC HNILQQSED KKAWKKLLI AAILCFFFM ITELIGGFI AGSLAVMTD AAHLFSDFI GFLISLLSI WVARKAPTR NMTFGYYRA EVLGAFLSV LTVWLLAGV FAVLAINRL LKKEYDIEA NTMMLVASL GLVVNIVMG AVLFGFCHN HSHGLSDQS NSNINVRAA AAHVLGDLL QSLGVLIAS IIIKVFPSA KVADPICTL IFSAVVIFT TAKVAKDSI WLLLEGSPK HSGDLAFEL LNISNVRHL HNLHIWALS PGKDAVSVH LCVDKYSNR DSILKKANS LIKSRLNVI SCTIQIETY SLELINSYK FVIFPIYLF ILLQ
- >TC009310 RA
atggagaca ctgtgtgag catgttgac gccgacgac gaaaatttc ctcctcgat gatgaaaac gctatcaaa agctgtaat aaatgcaaa agtccttcc aatggtttt gtaacaatg gacgaccac gttgacacc cttgaagat tcaccactt ctcatcaat gattgctat catgacgaa ttccattgc cacaacatt ttgcaacaa tccgaagac aaaaaagcc tggaagaaa ctcctaatt gcggcaatt ctgtgcttc ttttttatg attacggaa ctaattggt ggttttatt gccggtagt cttgccgta atgacagat gcggcgcat cttttctca gattttatc gggtttctt atttcgctt ttatcaatt tgggtggca cgtaaagct cccacaaga aatatgact tttgggtat tacagagca gaggttttg ggcgcattt ttgagcgtt ttaaccgtt tggttactc gccggcgtt tttgcagta cttgcaatt aacagattg ttgaagaaa gaatacgat attgaagct aatactatg atgttggtg gcctcatta ggccttgtt gtaaatatt gtgatgggg gctgtgctt ttcggcttt tgtcacaac cacagtcat ggccttagc gaccaaagc aattcgaat attaacgtg cgagctgca gcagctcac gttctgggc gacctttta caaagttta ggggtgctt atagcttca attattatt aaagtgttt cctagtgcc aaagtggcc gacccgatt tgcacttta attttttca gcggttgtt attttcaca actgctaaa gttgcaaaa gattccatt tggttgtta ctggaaggc agtcccaaa cattccggt gatctagca tttgagtta ttaaatatt tcgaatgtt agacatctt cataattta catatctgg gctctatct cctgggaag gatgccgtt tcagtccat ttgtgtgtt gataaatac tctaatcgt gattctatt ttaaagaaa gctaattca ctcattaag agtcgatta aatgtgata agttgtact attcagatt gaaacttat agtttagag ttgattaat tcgtacaaa gagttgcga gttcgtgta gatcctctg aaaatcgtc tactga
- >TC009310 RB
atggagaca ctgtgtgag catgttgac gccgacgac gaaaatttc ctcctcgat gatgaaaac gctatcaaa agctgtaat aaatgcaaa agtccttcc aatggtttt gtaacaatg gacgaccac gttgacacc cttgaagat tcaccactt ctcatcaat gattgctat catgacgaa ttccattgc cacaacatt ttgcaacaa tccgaagac aaaaaagcc tggaagaaa ctcctaatt gcggcaatt ctgtgcttc ttttttatg attacggaa ctaattggt ggttttatt gccggtagt cttgccgta atgacagat gcggcgcat cttttctca gattttatc gggtttctt atttcgctt ttatcaatt tgggtggca cgtaaagct cccacaaga aatatgact tttgggtat tacagagca gaggttttg ggcgcattt ttgagcgtt ttaaccgtt tggttactc gccggcgtt tttgcagta cttgcaatt aacagattg ttgaagaaa gaatacgat attgaagct aatactatg atgttggtg gcctcatta ggccttgtt gtaaatatt gtgatgggg gctgtgctt ttcggcttt tgtcacaac cacagtcat ggccttagc gaccaaagc aattcgaat attaacgtg cgagctgca gcagctcac gttctgggc gacctttta caaagttta ggggtgctt atagcttca attattatt aaagtgttt cctagtgcc aaagtggcc gacccgatt tgcacttta attttttca gcggttgtt attttcaca actgctaaa gttgcaaaa gattccatt tggttgtta ctggaaggc agtcccaaa cattccggt gatctagca tttgagtta ttaaatatt tcgaatgtt agacatctt cataattta catatctgg gctctatct cctgggaag gatgccgtt tcagtccat ttgtgtgtt gataaatac tctaatcgt gattctatt ttaaagaaa gctaattca ctcattaag agtcgatta aatgtgata agttgtact attcagatt gaaacttat agtttagag ttgattaat tcgtacaag tttgtaatt tttccaatt tatttattt atattatta caataa
- >TC009310 RA
tatgagttg gcaaattaa aattagaat tagaatctc aaccattcc aaaccagat ttgcaattt ctgtttccg gtcgtttca gtccgccat ctgaaacaa cagtaatta ctacacatt cgcacatgt tgaaaattt tcaacgcaa ctaatttca tccaacaat gatgaaaat ttttggcga gttttcggc attcaatca ttttggaga taatgggca gtagagtgg gcgtgtttt ggtcagagg ttgcaaagt acatgtgtt tttgtcaga taatagata gtatatctc tggagggcg gcgacttgc tatttgtca taagcatcc tgccaacat tggtcatat gaattacgg tttcttatt cttgtaatg ttgttatct tgttcggac aggggacgt tatttattg cttcggttt gtgggagtt atgtacatt tttatgaca tatggacca aataaagac cttaccttt tttatgtca cttgccaag aaaacagac ttttttatc gaaacacag ttatatttc aaatacgag tattgttca tatttatta caattcgcg tagttttgt gagtgcaat gatggtacg aagggacaa tttctttat ttataactt tataataca ttttttgtg tccatgatc ttaaactaa attcctatt ttaatccca ctgacgtgt cccgattct agttataat atttctata atagacaaa tagtgataa ttcagtctt aaaaatgtt aagtgttaa accaccatg gttggtgta gtttcaata gttgagaaa aatcgcttt atttgatca aatggagac actgtgtga gcatgttga cgccgacga cgaaaattt cctcctcga tgatgaaaa cgctatcaa aagctgtaa taaatgcaa aagtccttc caatggttt tgtaacaat ggacgacca cgttgacac ccttgaaga ttcaccact tctcatcaa tgattgcta tcatgacga attccattg ccacaacat tttgcaaca atccgaaga caaaaaagc ctggaagaa actcctaat tgcggcaat tctgtgctt cttttttat gattacgga actaattgg tggttttat tgccggtag tcttgccgt aatgacaga tgcggcgca tcttttctc agattttat cgggtttct tatttcgct tttatcaat ttgggtggc acgtaaagc tcccacaag aaatatgac ttttgggta ttacagagc agaggtttt gggcgcatt tttgagcgt tttaaccgt ttggttact cgccggcgt ttttgcagt acttgcaat taacagatt gttgaagaa agaatacga tattgaagc taatactat gatgttggt ggcctcatt aggccttgt tgtaaatat tgtgatggg ggctgtgct tttcggctt ttgtcacaa ccacagtca tggccttag cgaccaaag caattcgaa tattaacgt gcgagctgc agcagctca cgttctggg cgacctttt acaaagttt aggggtgct tatagcttc aattattat taaagtgtt tcctagtgc caaagtggc cgacccgat ttgcacttt aattttttc agcggttgt tattttcac aactgctaa agttgcaaa agattccat ttggttgtt actggaagg cagtcccaa acattccgg tgatctagc atttgagtt attaaatat ttcgaatgt tagacatct tcataattt acatatctg ggctctatc tcctgggaa ggatgccgt ttcagtcca tttgtgtgt tgataaata ctctaatcg tgattctat tttaaagaa agctaattc actcattaa gagtcgatt aaatgtgat aagttgtac tattcagat tgaaactta tagtttaga gttgattaa ttcgtacaa agagttgcg agttcgtgt agatcctct gaaaatcgt ctactgacc acattatta cttatatca caatcacct gcgtaaaaa tagtaaatt acaaccaat tatactaaa aatttcaaa accaaccat gaacttcgt tattttcat ctgtaggta cagtagtac agtccaaca gttgtggca cacagcctc tcacttttt ccgtcaaca ttaatgtcg ttgagactc cagcgtatg gttctgcac cgtttgcca actcacaaa ttaacatac ccacactat aaatgtctg atttttgat tatagcctt gaacaaatt taattaaaa caaaactat caaataaaa taaaatacc ctgcaaatt ttgttccaa aagttcggg acttagcca gtttaggtt cctcacagt gcttgctgg aaacgagtg aatttgcct ttgccattt tccatttaa aacaatcgg acaggcgta tcgtaagcc agcaagaga cgctttccc tgtcgcaga aattaaaat gtgaccagc tctgactga ccttgaata atttggctc aatactcgt tcacctaaa gcaacaaat taagcaacc tgtgaataa accccctct tgtgtatgt aatccaacg catcaagga catccttta aaattaaaa taatagcgt gttcaggca gtc
- >TC009310 RB
tatgagttg gcaaattaa aattagaat tagaatctc aaccattcc aaaccagat ttgcaattt ctgtttccg gtcgtttca gtccgccat ctgaaacaa cagtaatta ctacacatt cgcacatgt tgaaaattt tcaacgcaa ctaatttca tccaacaat gatgaaaat ttttggcga gttttcggc attcaatca ttttggaga taatgggca gtagagtgg gcgtgtttt ggtcagagg ttgcaaagt acatgtgtt tttgtcaga taatagata gtatatctc tggagggcg gcgacttgc tatttgtca taagcatcc tgccaacat tggtcatat gaattacgg tttcttatt cttgtaatg ttgttatct tgttcggac aggggacgt tatttattg cttcggttt gtgggagtt atgtacatt tttatgaca tatggacca aataaagac cttaccttt tttatgtca cttgccaag aaaacagac ttttttatc gaaacacag ttatatttc aaatacgag tattgttca tatttatta caattcgcg tagttttgt gagtgcaat gatggtacg aagggacaa tttctttat ttataactt tataataca ttttttgtg tccatgatc ttaaactaa attcctatt ttaatccca ctgacgtgt cccgattct agttataat atttctata atagacaaa tagtgataa ttcagtctt aaaaatgtt aagtgttaa accaccatg gttggtgta gtttcaata gttgagaaa aatcgcttt atttgatca aatggagac actgtgtga gcatgttga cgccgacga cgaaaattt cctcctcga tgatgaaaa cgctatcaa aagctgtaa taaatgcaa aagtccttc caatggttt tgtaacaat ggacgacca cgttgacac ccttgaaga ttcaccact tctcatcaa tgattgcta tcatgacga attccattg ccacaacat tttgcaaca atccgaaga caaaaaagc ctggaagaa actcctaat tgcggcaat tctgtgctt cttttttat gattacgga actaattgg tggttttat tgccggtag tcttgccgt aatgacaga tgcggcgca tcttttctc agattttat cgggtttct tatttcgct tttatcaat ttgggtggc acgtaaagc tcccacaag aaatatgac ttttgggta ttacagagc agaggtttt gggcgcatt tttgagcgt tttaaccgt ttggttact cgccggcgt ttttgcagt acttgcaat taacagatt gttgaagaa agaatacga tattgaagc taatactat gatgttggt ggcctcatt aggccttgt tgtaaatat tgtgatggg ggctgtgct tttcggctt ttgtcacaa ccacagtca tggccttag cgaccaaag caattcgaa tattaacgt gcgagctgc agcagctca cgttctggg cgacctttt acaaagttt aggggtgct tatagcttc aattattat taaagtgtt tcctagtgc caaagtggc cgacccgat ttgcacttt aattttttc agcggttgt tattttcac aactgctaa agttgcaaa agattccat ttggttgtt actggaagg cagtcccaa acattccgg tgatctagc atttgagtt attaaatat ttcgaatgt tagacatct tcataattt acatatctg ggctctatc tcctgggaa ggatgccgt ttcagtcca tttgtgtgt tgataaata ctctaatcg tgattctat tttaaagaa agctaattc actcattaa gagtcgatt aaatgtgat aagttgtac tattcagat tgaaactta tagtttaga gttgattaa ttcgtacaa gtttgtaat ttttccaat ttatttatt tatattatt acaataagt gtcattgga agagcaatc actgtccaa atagctgtt tttataata ctgaaataa agtgattga ctgaattgt ctttttaaa aatcccatt cgcacgaat ataactcca agtcggcga attgttgca caacgtcca aatttgcaa cttcgtcta taaaaacca aaataagaa cttttcaga cacattttg atggcccgg agcagccac ccaagtcac acacgttta cggaaaact gattcttac ggaaaattt tttgatgaa ttcgaatct gtagatggt attgcattc attatgaaa aatttagaa aaaacaata tttgacaaa ttttgtgtt tacattgtc atgtaaaaa aattcagtt ttcttgaaa cgtttcata tttgtgtag tgaaatcct taccaagtt attgcagtt tcaagttgg tgcaataac caaagctcg cacggtaaa ataccccgc agcgatttt cccgttagc agttcattt tttctatga caaatattt caaaggcca aaaaacgct aaataatca aaaaacaat tataattga aacctcaaa gtacagttt atttactaa ctaaatgct tgaaattgg ttaaaattc aacactttt cttgaatca gttttcagt aatcgagtg cggacgaat ttcgtaata agcgtcttt taatcaagc gcctgctcc cggactaat tacagttac ctgtattaa cagctacgc gactactaa gcggcataa caggcttaa gtaaatcag gcaaaaaga ttcctttct ttattaatt taaagatag ggtgtgtga ggagttgac tagcggtgg gacggataa ttgcctcct tttgtagac aaagagttg cgagttcgt gtagatcct ctgaaaatc gtctactga ccacattat tacttatat cacaatcac ctgcgtaaa aatagtaaa ttacaacca attatacta aaaatttca aaaccaacc atgaacttc gttattttc atctgtagg tacagtagt acagtccaa cagttgtgg cacacagcc tctcacttt ttccgtcaa cattaatgt cgttgagac tccagcgta tggttctgc accgtttgc caactcaca aattaacat acccacact ataaatgtc tgatttttg attatagcc ttgaacaaa tttaattaa aacaaaact atcaaataa aataaaata ccctgcaaa ttttgttcc aaaagttcg ggacttagc cagtttagg ttcctcaca gtgcttgct ggaaacgag tgaatttgc ctttgccat tttccattt aaaacaatc ggacaggcg tatcgtaag ccagcaaga gacgctttc cctgtcgca gaaattaaa atgtgacca gctctgact gaccttgaa taatttggc tcaatactc gttcaccta aagcaacaa attaagcaa cctgtgaat aaaccccct cttgtgtat gtaatccaa cgcatcaag gacatcctt taaaattaa aataatagc gtgttcagg cagtc
