General information | |||
TC009308 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 182762..186559 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009308 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06267 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC009308 RA
MSNKSTTLN VSGDLSLAS IDFPKFSKN VIVSEELNA YLRDQLQQT KRENHTLHE ELARKESQI EQNQRKIDK LNEKLHDFE NCCSNDFAS ASQVANSKI VELSKKLRE KNSEIEVYK SKCIKLENT LSELKLKQE DTVQIDKPV ETDLDETKK LQEKLNYTT NKWIEEKNL NLQLKNDLK RANKWLQQE VGDKFETLL SLNNSSNSN WRGRAQIIC DLQQKNQEL KEKLKQFQD NKSPHLNTE ALQVKHERK IEALKEENS NLKNNYTDL KRKFDTLKT RCKVLEGDQ SFLKSKLAS LTERHDADQ QTVVKLTSQ LQQCNHLKT DDLRQKDQL VKHLQQEIE SLKVELMKE KCLSENKSK ELLEKSDDI QLLNKRIRS SRPPSSYKN RHENLESKT INRLEVERL RLLELSQVQ NDRLEMERN AHLKSQHLL RAEKQKCAR LEASLARLE LEQSSAKIG YANKNMTPK RFENDIKDK LELAEENIK ALTTRLEIE SMERKRDFQ EFSKILKDC TCGKF
- >TC009308 RA
atgagcaac aaatccaca actttaaat gtttctgga gatttgagt cttgcttca atcgatttt ccaaaattt tccaaaaat gtaatagtt tccgaagaa cttaacgcc tatttgcgc gatcaactc cagcaaacg aaacgtgaa aatcatact ctacatgag gaattggcg agaaaagag tctcagatt gaacaaaat caaagaaaa atcgataaa ctaaacgaa aagcttcac gatttcgaa aattgttgc tctaacgac tttgcttcc gcttcgcaa gttgcaaat agcaaaatt gtagaactg agtaaaaaa ttaagagag aaaaattcg gaaattgaa gtgtacaaa tcgaaatgt ataaaactt gaaaacact ttatccgaa ttaaaatta aaacaagag gacactgtt caaatcgat aaaccagtc gagactgat ttagatgaa acaaaaaaa ctacaagag aaactcaac tacactaca aacaaatgg atcgaagaa aaaaattta aatttgcaa ctgaaaaac gacctcaaa cgtgccaat aagtggttg cagcaagaa gtcggtgat aagtttgaa actctcttg agcctaaat aactcatcc aattcgaat tggagaggc agagcgcag ataatttgc gacttgcaa cagaaaaat caggaactg aaagaaaag ctaaagcag tttcaggac aataaaagt cctcatttg aatactgag gcgctccaa gtcaaacac gaaagaaaa attgaggcg ttaaaagag gaaaactct aatctgaaa aataattat acggacctg aagcgaaaa tttgatact ctcaagact aggtgcaag gtgttggaa ggcgatcag agcttcctg aaatcaaaa ctagccagt ttgaccgaa agacatgac gccgaccaa caaactgtg gtcaaatta acgtcgcag ttgcagcaa tgtaaccac cttaaaaca gacgatttg cggcaaaag gaccaactg gtgaaacat ttgcaacag gagattgag tcgctcaaa gtcgagtta atgaaagaa aaatgtttg tctgagaac aaatcgaaa gaactgctg gaaaaaagc gatgatatt cagttactc aataagaga ataagaagt tcacggcct ccaagttcc tacaaaaac agacatgaa aacctggaa tctaaaact ataaaccgg ctcgaagtg gagcgtttg cgtcttttg gagttgagt caagtccaa aatgatcga cttgaaatg gagagaaac gctcatctt aaaagccag caccttctc cgtgctgaa aaacagaaa tgtgcgagg cttgaggcg agtttggcg agactggaa ttggaacaa agtagtgca aaaattggt tatgcaaat aaaaatatg actccgaaa cggtttgag aatgatatt aaagacaaa ctggaattg gctgaggaa aatatcaaa gctttgacg actagactg gaaatcgag agtatggaa aggaaacgg gattttcag gagtttagt aagattttg aaggactgt acttgtggc aaattttga
- >TC009308 RA
attctcaaa ttttaaaag tggatgcaa gtgaaagat attagaacg agttatcgt attaattca atttattaa tgaagcata aaatacagt aatttcatt tcttctttt tttgttgct tctgacgac gtttttcct cttttcttt tttcacgcg ctgtcaatg acttgtaca gtttgtaac caaagaatg ctgaaaaag atgacaaac gttcagaaa aatgaaacc caaatacca gtaataatt gatttacca aaaatcaca accagtaac acaacacaa aaaatgaaa gcgtgagca taatagtta actctcgtt tggagaagt tgtcccaat ttgtcttta aaatcatcc ccgaattct tctttcaaa gcattggcc acatttttt atcttctcc gaaagttcg tcaacgtca agattattt ttttttatt tcattcaaa tttgccatt ttaggccac actttattg ccacttaac aagcaacaa ccgaattta ctttactct gttatctgt gattgtgta atgtgatcg agatataaa atttattaa ctcttatcg taggtaatt gtaattatt ttcttaccg aaaattaaa ccgacaacg gttatcaaa ccgaaaaga attgctaag tctggagca gttccaaaa gtgggaccg ttcattgga ggcatgaat ccaggtttt gcttcattg gcgcgtgcg atcaagttg tctctcagt ttttctttt tcttcctct gggatgcct tccatattt tgcaaagct tctaatagc tcttccaac gttggcatt ttgaaattg ccatcccct ggcgcgtta ttgactgct ccaggttcc attttaaga cactcacgc acttatcaa tttgataag gaaaatgct gttttggga ggcaataat aaaaataaa tgcaacgtg ttagacatg tcatcagtt tttattttg caaggaatg ttatcgcgg tttagtgtt ctacttgat acaggaacg tattacaat tttttgtgt tttgacatt tgttaaatg catgaccaa attatataa taaattaaa aaaatttat tcatcaact gtacaataa aatatgaag aatacaaca tgacaatgg aatgtttta aaattattt agggtccct gtccctact ttttcttct tttgttgct tttgtcgcc gtttttctt ctttccttc gttcacgtt cagccaagg acttgtaca atttatagc caaagaaaa ctgtaacaa aaattcaaa aattgacac ttgacgttt gacactttt tatcatcat tgcacacga gggttacaa attttaaaa ttattattg atgaattgg aagtgtacg gaaatatta attaaaatt ttacttact cgacaaaga tacgaaaat tataccggg caaaataaa aaccgagtt tgagaaacc atgttcgat aggaccgtc ttgcccacc tcttaaatc aaccatagt ataatatag taattttaa atgcaacca ataatctac acttgttgt ttaactaaa aaattcaat aatttgccg cttctatta tttattgat aacaagaac aaatctacg gttatctct tcccatcca agtgacatt ggaagaaac ataaaatcc ctaattgac gtgggaaat tcaaacaaa aaaagtaac tgtcaagta ttcaaaatg agcaacaaa tccacaact ttaaatgtt tctggagat ttgagtctt gcttcaatc gattttcca aaattttcc aaaaatgta atagtttcc gaagaactt aacgcctat ttgcgcgat caactccag caaacgaaa cgtgaaaat catactcta catgaggaa ttggcgaga aaagagtct cagattgaa caaaatcaa agaaaaatc gataaacta aacgaaaag cttcacgat ttcgaaaat tgttgctct aacgacttt gcttccgct tcgcaagtt gcaaatagc aaaattgta gaactgagt aaaaaatta agagagaaa aattcggaa attgaagtg tacaaatcg aaatgtata aaacttgaa aacacttta tccgaatta aaattaaaa caagaggac actgttcaa atcgataaa ccagtcgag actgattta gatgaaaca aaaaaacta caagagaaa ctcaactac actacaaac aaatggatc gaagaaaaa aatttaaat ttgcaactg aaaaacgac ctcaaacgt gccaataag tggttgcag caagaagtc ggtgataag tttgaaact ctcttgagc ctaaataac tcatccaat tcgaattgg agaggcaga gcgcagata atttgcgac ttgcaacag aaaaatcag gaactgaaa gaaaagcta aagcagttt caggacaat aaaagtcct catttgaat actgaggcg ctccaagtc aaacacgaa agaaaaatt gaggcgtta aaagaggaa aactctaat ctgaaaaat aattatacg gacctgaag cgaaaattt gatactctc aagactagg tgcaaggtg ttggaaggc gatcagagc ttcctgaaa tcaaaacta gccagtttg accgaaaga catgacgcc gaccaacaa actgtggtc aaattaacg tcgcagttg cagcaatgt aaccacctt aaaacagac gatttgcgg caaaaggac caactggtg aaacatttg caacaggag attgagtcg ctcaaagtc gagttaatg aaagaaaaa tgtttgtct gagaacaaa tcgaaagaa ctgctggaa aaaagcgat gatattcag ttactcaat aagagaata agaagttca cggcctcca agttcctac aaaaacaga catgaaaac ctggaatct aaaactata aaccggctc gaagtggag cgtttgcgt cttttggag ttgagtcaa gtccaaaat gatcgactt gaaatggag agaaacgct catcttaaa agccagcac cttctccgt gctgaaaaa cagaaatgt gcgaggctt gaggcgagt ttggcgaga ctggaattg gaacaaagt agtgcaaaa attggttat gcaaataaa aatatgact ccgaaacgg tttgagaat gatattaaa gacaaactg gaattggct gaggaaaat atcaaagct ttgacgact agactggaa atcgagagt atggaaagg aaacgggat tttcaggag tttagtaag attttgaag gactgtact tgtggcaaa ttttgagta aatacccac tgataatgt tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
