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Gene information
General information
TC009308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 182762..186559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009308
    iBeetle screen iB_06267

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009308 RA
      MSNKSTTLN VSGDLSLAS IDFPKFSKN VIVSEELNA 
      YLRDQLQQT KRENHTLHE ELARKESQI EQNQRKIDK 
      LNEKLHDFE NCCSNDFAS ASQVANSKI VELSKKLRE 
      KNSEIEVYK SKCIKLENT LSELKLKQE DTVQIDKPV 
      ETDLDETKK LQEKLNYTT NKWIEEKNL NLQLKNDLK 
      RANKWLQQE VGDKFETLL SLNNSSNSN WRGRAQIIC 
      DLQQKNQEL KEKLKQFQD NKSPHLNTE ALQVKHERK 
      IEALKEENS NLKNNYTDL KRKFDTLKT RCKVLEGDQ 
      SFLKSKLAS LTERHDADQ QTVVKLTSQ LQQCNHLKT 
      DDLRQKDQL VKHLQQEIE SLKVELMKE KCLSENKSK 
      ELLEKSDDI QLLNKRIRS SRPPSSYKN RHENLESKT 
      INRLEVERL RLLELSQVQ NDRLEMERN AHLKSQHLL 
      RAEKQKCAR LEASLARLE LEQSSAKIG YANKNMTPK 
      RFENDIKDK LELAEENIK ALTTRLEIE SMERKRDFQ 
      EFSKILKDC TCGKF
    CDS sequence
    • >TC009308 RA
      atgagcaac aaatccaca actttaaat gtttctgga 
      gatttgagt cttgcttca atcgatttt ccaaaattt 
      tccaaaaat gtaatagtt tccgaagaa cttaacgcc 
      tatttgcgc gatcaactc cagcaaacg aaacgtgaa 
      aatcatact ctacatgag gaattggcg agaaaagag 
      tctcagatt gaacaaaat caaagaaaa atcgataaa 
      ctaaacgaa aagcttcac gatttcgaa aattgttgc 
      tctaacgac tttgcttcc gcttcgcaa gttgcaaat 
      agcaaaatt gtagaactg agtaaaaaa ttaagagag 
      aaaaattcg gaaattgaa gtgtacaaa tcgaaatgt 
      ataaaactt gaaaacact ttatccgaa ttaaaatta 
      aaacaagag gacactgtt caaatcgat aaaccagtc 
      gagactgat ttagatgaa acaaaaaaa ctacaagag 
      aaactcaac tacactaca aacaaatgg atcgaagaa 
      aaaaattta aatttgcaa ctgaaaaac gacctcaaa 
      cgtgccaat aagtggttg cagcaagaa gtcggtgat 
      aagtttgaa actctcttg agcctaaat aactcatcc 
      aattcgaat tggagaggc agagcgcag ataatttgc 
      gacttgcaa cagaaaaat caggaactg aaagaaaag 
      ctaaagcag tttcaggac aataaaagt cctcatttg 
      aatactgag gcgctccaa gtcaaacac gaaagaaaa 
      attgaggcg ttaaaagag gaaaactct aatctgaaa 
      aataattat acggacctg aagcgaaaa tttgatact 
      ctcaagact aggtgcaag gtgttggaa ggcgatcag 
      agcttcctg aaatcaaaa ctagccagt ttgaccgaa 
      agacatgac gccgaccaa caaactgtg gtcaaatta 
      acgtcgcag ttgcagcaa tgtaaccac cttaaaaca 
      gacgatttg cggcaaaag gaccaactg gtgaaacat 
      ttgcaacag gagattgag tcgctcaaa gtcgagtta 
      atgaaagaa aaatgtttg tctgagaac aaatcgaaa 
      gaactgctg gaaaaaagc gatgatatt cagttactc 
      aataagaga ataagaagt tcacggcct ccaagttcc 
      tacaaaaac agacatgaa aacctggaa tctaaaact 
      ataaaccgg ctcgaagtg gagcgtttg cgtcttttg 
      gagttgagt caagtccaa aatgatcga cttgaaatg 
      gagagaaac gctcatctt aaaagccag caccttctc 
      cgtgctgaa aaacagaaa tgtgcgagg cttgaggcg 
      agtttggcg agactggaa ttggaacaa agtagtgca 
      aaaattggt tatgcaaat aaaaatatg actccgaaa 
      cggtttgag aatgatatt aaagacaaa ctggaattg 
      gctgaggaa aatatcaaa gctttgacg actagactg 
      gaaatcgag agtatggaa aggaaacgg gattttcag 
      gagtttagt aagattttg aaggactgt acttgtggc 
      aaattttga
    mRNA sequence
    • >TC009308 RA
      attctcaaa ttttaaaag tggatgcaa gtgaaagat 
      attagaacg agttatcgt attaattca atttattaa 
      tgaagcata aaatacagt aatttcatt tcttctttt 
      tttgttgct tctgacgac gtttttcct cttttcttt 
      tttcacgcg ctgtcaatg acttgtaca gtttgtaac 
      caaagaatg ctgaaaaag atgacaaac gttcagaaa 
      aatgaaacc caaatacca gtaataatt gatttacca 
      aaaatcaca accagtaac acaacacaa aaaatgaaa 
      gcgtgagca taatagtta actctcgtt tggagaagt 
      tgtcccaat ttgtcttta aaatcatcc ccgaattct 
      tctttcaaa gcattggcc acatttttt atcttctcc 
      gaaagttcg tcaacgtca agattattt ttttttatt 
      tcattcaaa tttgccatt ttaggccac actttattg 
      ccacttaac aagcaacaa ccgaattta ctttactct 
      gttatctgt gattgtgta atgtgatcg agatataaa 
      atttattaa ctcttatcg taggtaatt gtaattatt 
      ttcttaccg aaaattaaa ccgacaacg gttatcaaa 
      ccgaaaaga attgctaag tctggagca gttccaaaa 
      gtgggaccg ttcattgga ggcatgaat ccaggtttt 
      gcttcattg gcgcgtgcg atcaagttg tctctcagt 
      ttttctttt tcttcctct gggatgcct tccatattt 
      tgcaaagct tctaatagc tcttccaac gttggcatt 
      ttgaaattg ccatcccct ggcgcgtta ttgactgct 
      ccaggttcc attttaaga cactcacgc acttatcaa 
      tttgataag gaaaatgct gttttggga ggcaataat 
      aaaaataaa tgcaacgtg ttagacatg tcatcagtt 
      tttattttg caaggaatg ttatcgcgg tttagtgtt 
      ctacttgat acaggaacg tattacaat tttttgtgt 
      tttgacatt tgttaaatg catgaccaa attatataa 
      taaattaaa aaaatttat tcatcaact gtacaataa 
      aatatgaag aatacaaca tgacaatgg aatgtttta 
      aaattattt agggtccct gtccctact ttttcttct 
      tttgttgct tttgtcgcc gtttttctt ctttccttc 
      gttcacgtt cagccaagg acttgtaca atttatagc 
      caaagaaaa ctgtaacaa aaattcaaa aattgacac 
      ttgacgttt gacactttt tatcatcat tgcacacga 
      gggttacaa attttaaaa ttattattg atgaattgg 
      aagtgtacg gaaatatta attaaaatt ttacttact 
      cgacaaaga tacgaaaat tataccggg caaaataaa 
      aaccgagtt tgagaaacc atgttcgat aggaccgtc 
      ttgcccacc tcttaaatc aaccatagt ataatatag 
      taattttaa atgcaacca ataatctac acttgttgt 
      ttaactaaa aaattcaat aatttgccg cttctatta 
      tttattgat aacaagaac aaatctacg gttatctct 
      tcccatcca agtgacatt ggaagaaac ataaaatcc 
      ctaattgac gtgggaaat tcaaacaaa aaaagtaac 
      tgtcaagta ttcaaaatg agcaacaaa tccacaact 
      ttaaatgtt tctggagat ttgagtctt gcttcaatc 
      gattttcca aaattttcc aaaaatgta atagtttcc 
      gaagaactt aacgcctat ttgcgcgat caactccag 
      caaacgaaa cgtgaaaat catactcta catgaggaa 
      ttggcgaga aaagagtct cagattgaa caaaatcaa 
      agaaaaatc gataaacta aacgaaaag cttcacgat 
      ttcgaaaat tgttgctct aacgacttt gcttccgct 
      tcgcaagtt gcaaatagc aaaattgta gaactgagt 
      aaaaaatta agagagaaa aattcggaa attgaagtg 
      tacaaatcg aaatgtata aaacttgaa aacacttta 
      tccgaatta aaattaaaa caagaggac actgttcaa 
      atcgataaa ccagtcgag actgattta gatgaaaca 
      aaaaaacta caagagaaa ctcaactac actacaaac 
      aaatggatc gaagaaaaa aatttaaat ttgcaactg 
      aaaaacgac ctcaaacgt gccaataag tggttgcag 
      caagaagtc ggtgataag tttgaaact ctcttgagc 
      ctaaataac tcatccaat tcgaattgg agaggcaga 
      gcgcagata atttgcgac ttgcaacag aaaaatcag 
      gaactgaaa gaaaagcta aagcagttt caggacaat 
      aaaagtcct catttgaat actgaggcg ctccaagtc 
      aaacacgaa agaaaaatt gaggcgtta aaagaggaa 
      aactctaat ctgaaaaat aattatacg gacctgaag 
      cgaaaattt gatactctc aagactagg tgcaaggtg 
      ttggaaggc gatcagagc ttcctgaaa tcaaaacta 
      gccagtttg accgaaaga catgacgcc gaccaacaa 
      actgtggtc aaattaacg tcgcagttg cagcaatgt 
      aaccacctt aaaacagac gatttgcgg caaaaggac 
      caactggtg aaacatttg caacaggag attgagtcg 
      ctcaaagtc gagttaatg aaagaaaaa tgtttgtct 
      gagaacaaa tcgaaagaa ctgctggaa aaaagcgat 
      gatattcag ttactcaat aagagaata agaagttca 
      cggcctcca agttcctac aaaaacaga catgaaaac 
      ctggaatct aaaactata aaccggctc gaagtggag 
      cgtttgcgt cttttggag ttgagtcaa gtccaaaat 
      gatcgactt gaaatggag agaaacgct catcttaaa 
      agccagcac cttctccgt gctgaaaaa cagaaatgt 
      gcgaggctt gaggcgagt ttggcgaga ctggaattg 
      gaacaaagt agtgcaaaa attggttat gcaaataaa 
      aatatgact ccgaaacgg tttgagaat gatattaaa 
      gacaaactg gaattggct gaggaaaat atcaaagct 
      ttgacgact agactggaa atcgagagt atggaaagg 
      aaacgggat tttcaggag tttagtaag attttgaag 
      gactgtact tgtggcaaa ttttgagta aatacccac 
      tgataatgt tt
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