| General information | |||
| TC033567
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2369566..2463061 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033567 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06265 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva ventral potentiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen circumference irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva ventral potentiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen circumference irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC033567 RA
MSSAPIVWC WTALFFSSA WSGGLDMQG PLFLHEPPH RVEFSNISG AKVDCTAHG SPSPEVEWI LADGSSVHQ VPELRLLFP NGSLVFPPF PNERYRHDV HAALYRCKL KSSLGTVVS REVHVKAVV KQKYDVQVH DEYVIAGNT AVLKCKIPN YVAEYVMVT SWVQDETIN IYPNTDIGG KYVVLSNGD LYISNAGPG DGYKNYGCR TVNRLTGEV QTSMYPGRI IVTEPKGNV QPRISVDKY NARRVVLGE DVTLPCVAQ GHPVPGYYW KRELQGQSV PVALGERLT ILSAGLLRI SKVRLEDRG VYVCFANNS AGEESVRVT LEITAPLSA HVQPQVQVV DVGKEASFQ CIVNGYPVS QVSWLHNGK PVAPDHRVE VMAEPPRLT IRQLSKDDR GMYQCLASN NWDQAQATA ELDMGDAGP ELIYWFSEQ TLQPGPMVS LKCVATGNP PPQFTWTLD GFPLPDHPR FLVGQYVTI QDDVISHVN ITNIKAEDG GEYTCTAHN SVGKISHSA RVNVYGLPY IREMPKITG VAGGSLVIK CPVAGYPIE TISWEREGQ ILPVNRRQR VYANGTLVV EQTQRNEDA GTYTCQAQN RQRNSDRRN VEVQVIVPP KILPINPMT GVLREGMRA AITCQIMEG DLPITFRWE RNAKAVSNN VALGTVIRR IDEFSSSLI IEQVTSAHS GNYTCIASN VAGAEKHVV PLTVNVPPR WTVEPADSS VAAGQEATM HCQADGYPK PAVTWKKAV GEQPGEYKD FLFEPNVQQ FPNGSLNFL HISKENEGQ YLCEAKNNI GAGVSKVIF LKVNAPAHF LQKSKQLQV VKGEQAHLQ CSALGDTPM EITWKLGGQ HISNDGDQR YSVRAQQLA EGMVSELSI ERTIRQDTG IFTCSASNA YGSDDMNIQ LIVQEIPEP PRNVRVMDQ LSRSIGISW TQPYAGNSP ITNYIVQYK PGSEAWPNQ PAKVTVPGS QITTTLQNL RPAQVYHLR ILAENRLGL SDPSQVVQV STLEEVPSG APVDIRAEA KSSTELVVT WEPPNRETW NGNLLGYHV GYQEVSTED SNNVAQSYT FKSVEVRPH YGGEAVLQG LSKYTTYGI IVQAYNSRG SGPASDPVT ARTLEDAPS LPPLNVQCS VLSAQSLHI SWEPPLPEG RNGIIQGYK VTYHSAGEW FDNDDQQTK IINQLRTTI SGLRKFTNY SMTVLAYTS SGDGARSEP TYCQTEEDV PSAPAEIKA VLSAPDKIL VSWLPPKFS NGPLVGYTF YMSQMEDGR EEGTHKRVL SPSTEMHEV ERVKQSSTL EFWVTASTR IGEGESTRV ITVSPSKAV PARIASFGR EVISAWKQN VYLHCKRVG IPLPNAIWR VNDLPLEVG GRKTIFPNA TLVIKDTQS VDQANYSCS VENQYGRDE IEYSLKVLV PPEAPVLSV VEAFTDSLH LRWSDQGNG GSPILGYVI NYKRDHGDW EELQISART DEHMLRNLW CGTRYLLYI TAFNRIGTG LPCDIVGAH TKGTVPVKP KQSQMLTMN ATVVTIWLD SWGDGGCGI LYFVIEFRP GKHSPWIMS SNHVKPTER IYSISELWP ATEYQLKIT AHNNAGNTE ALYNFTTLT LLGAVPELA PPVSHSGDH PFYTNARVL VPIILSLLV LVALIATLF WRKKIRSER ESQNRSMGE SPSMAQIQN KQNRDQQYL AVRVGRGPQ PLAIDSDTY KAESADYIE DICPYATFQ LSKPAYSES SYSGNVYSG PYHSVRGSF VYHDVKPPP IDKFKLGHN KEPEYTKVR RKGGRLRDP HSESQESDN LGSTDSEVK KILTLHLPI SEYDTLGSD SEGDGRATS QELMSFSHR MRGGAEGSS SSSETSPPS GSVGRKPYP ARKGKAKGA PLSKRHVRS SSGYSSHND ETTFSISHA PSFNERIHP PSRFSDLNP RELSESDFD KGHKPRGMS KLTREAFQI NV
- >TC033567 RB
MSSAPIVWC WTALFFSSA WSGGLDMQG PLFLHEPPH RVEFSNISG AKVDCTAHG SPSPEVEWI LADGSSVHQ VPELRLLFP NGSLVFPPF PNERYRHDV HAALYRCKL KSSLGTVVS REVHVKAVV KQKYDVQVH DEYVIAGNT AVLKCKIPN YVAEYVMVT SWVQDETIN IYPNTDIGG KYVVLSNGD LYISNAGPG DGYKNYGCR TVNRLTGEV QTSMYPGRI IVTEPKGNV QPRISVDKY NARRVVLGE DVTLPCVAQ GHPVPGYYW KRELQGQSV PVALGERLT ILSAGLLRI SKVRLEDRG VYVCFANNS AGEESVRVT LEITAPLSA HVQPQVQVV DVGKEASFQ CIVNGYPVS QVSWLHNGK PVAPDHRVE VMAEPPRLT IRQLSKDDR GMYQCLASN NWDQAQATA ELDMGDAGP ELIYWFSEQ TLQPGPMVS LKCVATGNP PPQFTWTLD GFPLPDHPR FLVGQYVTI QDDVISHVN ITNIKAEDG GEYTCTAHN SVGKISHSA RVNVYGLPY IREMPKITG VAGGSLVIK CPVAGYPIE TISWEREGQ ILPVNRRQR VYANGTLVV EQTQRNEDA GTYTCQAQN RQRNSDRRN VEVQVIVPP KILPINPMT GVLREGMRA AITCQIMEG DLPITFRWE RNAKAVSNN VALGTVIRR IDEFSSSLI IEQVTSAHS GNYTCIASN VAGAEKHVV PLTVNVPPR WTVEPADSS VAAGQEATM HCQADGYPK PAVTWKKAV GEQPGEYKD FLFEPNVQQ FPNGSLNFL HISKENEGQ YLCEAKNNI GAGVSKVIF LKVNAPAHF LQKSKQLQV VKGEQAHLQ CSALGDTPM EITWKLGGQ HISNDGDQR YSVRAQQLA EGMVSELSI ERTIRQDTG IFTCSASNA YGSDDMNIQ LIVQEIPEP PRNVRVMDQ LSRSIGISW TQPYAGNSP ITNYIVQYK PGSEAWPNQ PAKVTVPGS QITTTLQNL RPAQVYHLR ILAENRLGL SDPSQVVQV STLEEVPSG APVDIRAEA KSSTELVVT WEPPNRETW NGNLLGYHV GYQEVSTED SNNVAQSYT FKSVEVRPH YGGEAVLQG LSKYTTYGI IVQAYNSRG SGPASDPVT ARTLEDAPS LPPLNVQCS VLSAQSLHI SWEPPLPEG RNGIIQGYK VTYHSAGEW FDNDDQQTK IINQLRTTI SGLRKFTNY SMTVLAYTS SGDGARSEP TYCQTEEDV PSAPAEIKA VLSAPDKIL VSWLPPKFS NGPLVGYTF YMSQMEDGR EEGTHKRVL SPSTEMHEV ERVKQSSTL EFWVTASTR IGEGESTRV ITVSPSKAV PARIASFGR EVISAWKQN VYLHCKRVG IPLPNAIWR VNDLPLEVG GRKTIFPNA TLVIKDTQS VDQANYSCS VENQYGRDE IEYSLKVLV PPEAPVLSV VEAFTDSLH LRWSDQGNG GSPILGYVI NYKRDHGDW EELQISART DEHMLRNLW CGTRYLLYI TAFNRIGTG LPCDIVGAH TKGTVPVKP KQSQMLTMN ATVVTIWLD SWGDGGCGI LYFVIEFRP GKHSPWIMS SNHVKPTER IYSISELWP ATEYQLKIT AHNNAGNTE ALYNFTTLT LLAVPELAP PVSHSGDHP FYTNARVLV PIILSLLVL VALIATLFW RKKIRSERE SQNRSMGES PSMAQIQNK QNRDQQYLA VRVGRGPQP LAIDSDTYK AESADYIED ICPYATFQL SKPAYSESS YSGNVYSGP YHSVRGSFV YHDVKPPPI DKFKLGHNK EPEYTKVRR KGGRLRDPH SESQESDNL GSTDSEVKK ILTLHLPIS EYDTLGSDS EGDGRATSQ ELMSFSHRM RGGAEGSSS SSETSPPSG SVGRKPYPA RKGKAKGAP LSKRHVRSS SGYSSHNDE TTFSISHAP SFNERIHPP SRFSDLNPR ELSESDFDK GHKPRGMSK LTREAFQIN V
- >TC033567 RA
atgagcagt gccccgatt gtatggtgc tggaccgcg ctcttcttc tcatcggca tggagtgga ggcttggac atgcaagga cctttgttc ttgcacgaa ccgccacac agagtggaa tttagcaac atttcgggc gcgaaagtg gactgcaca gcacatggg agtccttct ccggaggtg gagtggatt ctggcggat gggtcctca gtgcatcag gtgcctgaa ctccgactg ttattcccc aacgggtct ttggtgttt cccccgttt ccgaacgag aggtaccga cacgacgtc cacgcagcg ctctacagg tgcaaactg aaaagttca ctagggacg gttgtcagc cgggaagtg catgttaaa gctgttgtg aagcaaaaa tacgacgtc caagtccat gacgaatac gtgattgcg ggcaacaca gcggtcctc aaatgcaag attccgaat tacgtggct gaatacgtg atggtcacc tcctgggtc caagacgaa accatcaac atctacccc aacacggac attggcggc aaatacgtc gtcctcagc aacggggac ctctacata agtaatgca ggacctggg gacgggtac aagaactac ggctgtcgc actgttaac cggctcaca ggtgaagtc cagacgagc atgtatccg ggaaggata atcgtgacg gaaccgaag ggcaacgtc cagccacgg atcagcgtg gacaagtac aatgccagg agggtggtc ctgggggag gacgtcacg ctgccgtgc gtcgcgcaa ggacatcct gttcctgga tactactgg aagagggaa ctgcaagga caaagcgta cctgttgct ctgggggaa agactgacc attttatcg gcaggatta ctgaggata tccaaggtg cggttggag gaccgcggg gtgtacgtg tgcttcgcc aacaactct gcgggcgag gagagcgtg agggtgacg ctggaaata acagcgccc ttgtcagca catgttcaa cctcaagta caagtggtc gacgtcggg aaggaggcc agcttccag tgcattgtc aacggatat cccgtctct caagtcagc tggctgcac aacggcaag cccgtggcg cccgaccac cgcgtggag gtgatggcg gagccccca cgtctcacc attcgccaa ttatcgaaa gacgaccgc ggaatgtac cagtgcctg gcgagcaac aactgggac caagcccaa gccactgcc gaactggac atgggcgac gccggtcct gagctgatc tattggttc tcagagcag acgctccag ccaggaccg atggtttcg ctgaaatgt gtcgcaaca ggaaacccg ccgcctcag ttcacgtgg actctggat gggtttccg cttccagac catcctcga tttttggtc gggcaatat gtcacgatc caggacgac gtgatcagc cacgtgaat atcaccaat atcaaggcg gaagatggg ggcgaatac acatgtacg gcgcataac agcgtcggg aaaatatca cacagtgcc agggtgaac gtctacggc ctcccctac ataagagaa atgccgaaa atcaccgga gtggccgga ggcagtctg gtgatcaaa tgtcccgtg gcgggatac cccatcgag accataagc tgggagcgc gaaggccaa atcctgccg gtgaatcgt cggcagcgg gtttacgcc aacggcact ctggtggtg gagcagacg cagaggaac gaggacgcc ggcacctac acgtgccag gcgcaaaat cggcagcgg aactccgac agaaggaac gtcgaggtg caagttata gtaccgcca aagatcctg cccataaat ccgatgacg ggtgtgttg cgagaggga atgcgtgcg gcaataacc tgccaaatt atggaagga gacttgccg ataacattc cggtgggag cgcaacgcc aaagccgtt tccaataac gtcgctctg ggaacggta ataaggcga atcgacgag ttttcctcg tctttaatt atcgaacag gtgacatcc gctcattcc ggaaattac acttgcatc gccagcaac gtggcgggg gcggagaag cacgtcgtg ccgctcact gtcaacgtg ccgccgagg tggacggtg gagcccgca gactccagc gtcgctgct ggacaggag gccaccatg cattgccag gcagatggg tatcccaag ccagctgtc acctggaaa aaggccgtg ggtgagcaa cccggcgaa tacaaagac ttcctgttc gagcccaac gtgcagcaa ttccccaac ggttcgtta aacttcctc cacatatca aaagaaaac gaagggcaa tacttatgc gaagcgaaa aacaatatc ggcgctggc gtcagcaaa gtcattttc ctcaaagtc aacgctccg gcccacttc ctgcagaaa tcgaagcag ctgcaggtg gtgaagggc gagcaggcc cacctgcag tgctcggcc ctcggcgac acccccatg gagatcacc tggaagctc gggggccaa cacatatcc aacgacggc gatcagcgc tactccgtg cgggcgcag caactggcc gaggggatg gtctccgag ctgagcatt gagaggacg atcagacag gacacgggg atttttacg tgttcggcg agcaacgcc tacggaagc gatgacatg aatattcag ctgatcgtg caggagatt ccagaaccg ccgaggaac gtcagggta atggaccag ttatcgaga tcgatcggc atatcttgg acgcagccg tacgccgga aatagcccc atcactaac tacattgtc cagtacaaa ccgggatca gaagcgtgg ccgaatcag ccggccaag gtgacagtt cccggctcg caaatcacc acgactttg cagaacctg cgccctgcc caagtatat cacctccgg attttggcg gagaaccgc ttgggtcta agcgacccg agtcaagtc gtccaagtg agcaccctg gaagaagtg ccgagcggg gcccctgtt gatataagg gccgaggcg aagagttca actgaattg gtggtcact tgggaaccc ccgaatcgg gaaacttgg aatggtaat ctcttaggc tatcatgtg gggtaccag gaggtctcg acggaagat tcgaacaat gtggcgcag tcctacacc tttaaaagc gtggaagtg aggccacat tatgggggc gaagccgtg ctgcaaggc ctgtccaag tacaccacg tatgggatt atagtacag gcttacaac agccgagga tccggccct gctagcgac cccgtaaca gcgcgaact ctggaagat gcgccaagt ttgccccct ctgaacgtt cagtgttcg gtattaagt gctcaaagt ttacacata tcatgggaa ccgccgttg ccggaagga cgaaacgga attatccaa ggatataaa gttacttat cactcggcc ggcgaatgg ttcgacaac gacgatcaa cagaccaag atcatcaat caattgcgt acaacaatc tccggattg aggaaattc accaattac agcatgaca gtgctggcc tacacttcg agcggagac ggggcgagg tccgagccc acctactgc cagaccgag gaggatgtg ccatctgca ccggcggaa attaaggcc gtcctaagt gcccccgat aaaatacta gtatcgtgg ctgccaccc aaattcagt aatgggcca ttagttgga tacacgttc tacatgagc caaatggag gacggccga gaggaaggg acccacaaa agggtgctg agcccctcc accgaaatg cacgaagtt gagagggtt aaacaatcg tcaacgctc gagttctgg gtcacggcc tcaacaagg atcggcgaa ggcgagagc acacgtgtc atcaccgtg agcccctcg aaagccgtc ccggcgcga atcgcctcc ttcgggcgt gaggtaata agcgcctgg aagcagaac gtgtacctg cactgcaag cgcgtcgga atccctctt ccgaacgca atttggcgc gtcaacgac ctacccctc gaagtcgga ggccgtaaa acgatcttc cccaacgcc acgcttgtg attaaagac actcaaagc gtggatcaa gccaactac tcgtgcagc gtcgaaaat caatacgga agagatgaa atcgaatac agtttgaag gttttagtg ccaccggaa gcgcccgtt ttaagcgtc gttgaagct ttcaccgat agcttacac ctgagatgg agcgatcag ggcaacggg ggatcaccg atactcgga tacgtgatc aattacaag cgagaccac ggggactgg gaggaattg caaatttcc gcaaggacc gacgagcac atgcttagg aatttgtgg tgtgggacc agatatctc ctctacatc acggccttc aacaggatt ggcacggga ttgccgtgc gatatcgtc ggagctcac acgaaagga acagtccct gtgaagccg aagcagtcg cagatgctg accatgaac gcgaccgtg gtgaccatc tggctggac tcgtggggc gacggcggc tgcggcatc ctctacttc gtgatagag tttcgcccc ggaaagcac tccccttgg atcatgtcg tccaaccac gtcaaaccc accgaacga atttacagc ataagcgaa ctatggcct gccaccgaa taccagtta aaaatcacc gctcacaac aacgccggc aacaccgaa gccctgtac aacttcacc acattaaca ctcttaggt gctgttcca gagctggca ccgcccgtc tcgcactcg ggggatcac ccattctac accaacgct cgggtccta gttcccatc attctgtcg ctgttggtg ctcgtggcg ctgatcgcg acgctcttc tggcgcaaa aagattcgg agcgagcgg gagagccag aatcggtcg atgggcgag tcgccctcc atggcccag atccagaat aagcagaac agagaccag cagtatctg gctgtgcgg gtgggacga ggaccgcag cctctggcc atcgattcg gacacgtat aaggccgaa tcggcggat tatatcgag gatatttgt ccctatgcc acgtttcaa ttgtccaaa ccagcgtac agtgaaagc tcgtacagc ggaaacgtg tacagtgga ccgtaccat tcggtacgg ggttctttt gtttatcat gatgttaaa ccaccacct attgataag tttaagtta ggacataat aaagaacca gaatatacc aaggttcga agaaaaggg ggaagactg agagatcca cactcagag agtcaagag tctgataac ctgggcagc actgactca gaagtgaag aagatacta actctgcac ctgcctatt tcagagtac gacaccctc ggttcggat tccgagggc gacggccgc gccaccagc caagaactc atgtctttt agccataga atgcgtggc ggcgccgaa ggctcgtcc tcctcctcg gagacgtcg cccccgtcc ggcagcgtg gggcgcaag ccctacccc gcgcgcaag ggcaaggcc aagggcgcc cccttgagc aagcggcac gtgcgcagc agcagcggg tattcgagt cacaacgac gagaccacc ttcagcatc tcgcacgcg ccgagcttc aacgaacgg atacatccc ccgagcaga ttctccgac ttgaacccc agagagctg agcgagagc gactttgac aaagggcac aagccgaga ggaatgtcc aaattgaca cgagaagct ttccaaatc aacgtttaa
- >TC033567 RB
atgagcagt gccccgatt gtatggtgc tggaccgcg ctcttcttc tcatcggca tggagtgga ggcttggac atgcaagga cctttgttc ttgcacgaa ccgccacac agagtggaa tttagcaac atttcgggc gcgaaagtg gactgcaca gcacatggg agtccttct ccggaggtg gagtggatt ctggcggat gggtcctca gtgcatcag gtgcctgaa ctccgactg ttattcccc aacgggtct ttggtgttt cccccgttt ccgaacgag aggtaccga cacgacgtc cacgcagcg ctctacagg tgcaaactg aaaagttca ctagggacg gttgtcagc cgggaagtg catgttaaa gctgttgtg aagcaaaaa tacgacgtc caagtccat gacgaatac gtgattgcg ggcaacaca gcggtcctc aaatgcaag attccgaat tacgtggct gaatacgtg atggtcacc tcctgggtc caagacgaa accatcaac atctacccc aacacggac attggcggc aaatacgtc gtcctcagc aacggggac ctctacata agtaatgca ggacctggg gacgggtac aagaactac ggctgtcgc actgttaac cggctcaca ggtgaagtc cagacgagc atgtatccg ggaaggata atcgtgacg gaaccgaag ggcaacgtc cagccacgg atcagcgtg gacaagtac aatgccagg agggtggtc ctgggggag gacgtcacg ctgccgtgc gtcgcgcaa ggacatcct gttcctgga tactactgg aagagggaa ctgcaagga caaagcgta cctgttgct ctgggggaa agactgacc attttatcg gcaggatta ctgaggata tccaaggtg cggttggag gaccgcggg gtgtacgtg tgcttcgcc aacaactct gcgggcgag gagagcgtg agggtgacg ctggaaata acagcgccc ttgtcagca catgttcaa cctcaagta caagtggtc gacgtcggg aaggaggcc agcttccag tgcattgtc aacggatat cccgtctct caagtcagc tggctgcac aacggcaag cccgtggcg cccgaccac cgcgtggag gtgatggcg gagccccca cgtctcacc attcgccaa ttatcgaaa gacgaccgc ggaatgtac cagtgcctg gcgagcaac aactgggac caagcccaa gccactgcc gaactggac atgggcgac gccggtcct gagctgatc tattggttc tcagagcag acgctccag ccaggaccg atggtttcg ctgaaatgt gtcgcaaca ggaaacccg ccgcctcag ttcacgtgg actctggat gggtttccg cttccagac catcctcga tttttggtc gggcaatat gtcacgatc caggacgac gtgatcagc cacgtgaat atcaccaat atcaaggcg gaagatggg ggcgaatac acatgtacg gcgcataac agcgtcggg aaaatatca cacagtgcc agggtgaac gtctacggc ctcccctac ataagagaa atgccgaaa atcaccgga gtggccgga ggcagtctg gtgatcaaa tgtcccgtg gcgggatac cccatcgag accataagc tgggagcgc gaaggccaa atcctgccg gtgaatcgt cggcagcgg gtttacgcc aacggcact ctggtggtg gagcagacg cagaggaac gaggacgcc ggcacctac acgtgccag gcgcaaaat cggcagcgg aactccgac agaaggaac gtcgaggtg caagttata gtaccgcca aagatcctg cccataaat ccgatgacg ggtgtgttg cgagaggga atgcgtgcg gcaataacc tgccaaatt atggaagga gacttgccg ataacattc cggtgggag cgcaacgcc aaagccgtt tccaataac gtcgctctg ggaacggta ataaggcga atcgacgag ttttcctcg tctttaatt atcgaacag gtgacatcc gctcattcc ggaaattac acttgcatc gccagcaac gtggcgggg gcggagaag cacgtcgtg ccgctcact gtcaacgtg ccgccgagg tggacggtg gagcccgca gactccagc gtcgctgct ggacaggag gccaccatg cattgccag gcagatggg tatcccaag ccagctgtc acctggaaa aaggccgtg ggtgagcaa cccggcgaa tacaaagac ttcctgttc gagcccaac gtgcagcaa ttccccaac ggttcgtta aacttcctc cacatatca aaagaaaac gaagggcaa tacttatgc gaagcgaaa aacaatatc ggcgctggc gtcagcaaa gtcattttc ctcaaagtc aacgctccg gcccacttc ctgcagaaa tcgaagcag ctgcaggtg gtgaagggc gagcaggcc cacctgcag tgctcggcc ctcggcgac acccccatg gagatcacc tggaagctc gggggccaa cacatatcc aacgacggc gatcagcgc tactccgtg cgggcgcag caactggcc gaggggatg gtctccgag ctgagcatt gagaggacg atcagacag gacacgggg atttttacg tgttcggcg agcaacgcc tacggaagc gatgacatg aatattcag ctgatcgtg caggagatt ccagaaccg ccgaggaac gtcagggta atggaccag ttatcgaga tcgatcggc atatcttgg acgcagccg tacgccgga aatagcccc atcactaac tacattgtc cagtacaaa ccgggatca gaagcgtgg ccgaatcag ccggccaag gtgacagtt cccggctcg caaatcacc acgactttg cagaacctg cgccctgcc caagtatat cacctccgg attttggcg gagaaccgc ttgggtcta agcgacccg agtcaagtc gtccaagtg agcaccctg gaagaagtg ccgagcggg gcccctgtt gatataagg gccgaggcg aagagttca actgaattg gtggtcact tgggaaccc ccgaatcgg gaaacttgg aatggtaat ctcttaggc tatcatgtg gggtaccag gaggtctcg acggaagat tcgaacaat gtggcgcag tcctacacc tttaaaagc gtggaagtg aggccacat tatgggggc gaagccgtg ctgcaaggc ctgtccaag tacaccacg tatgggatt atagtacag gcttacaac agccgagga tccggccct gctagcgac cccgtaaca gcgcgaact ctggaagat gcgccaagt ttgccccct ctgaacgtt cagtgttcg gtattaagt gctcaaagt ttacacata tcatgggaa ccgccgttg ccggaagga cgaaacgga attatccaa ggatataaa gttacttat cactcggcc ggcgaatgg ttcgacaac gacgatcaa cagaccaag atcatcaat caattgcgt acaacaatc tccggattg aggaaattc accaattac agcatgaca gtgctggcc tacacttcg agcggagac ggggcgagg tccgagccc acctactgc cagaccgag gaggatgtg ccatctgca ccggcggaa attaaggcc gtcctaagt gcccccgat aaaatacta gtatcgtgg ctgccaccc aaattcagt aatgggcca ttagttgga tacacgttc tacatgagc caaatggag gacggccga gaggaaggg acccacaaa agggtgctg agcccctcc accgaaatg cacgaagtt gagagggtt aaacaatcg tcaacgctc gagttctgg gtcacggcc tcaacaagg atcggcgaa ggcgagagc acacgtgtc atcaccgtg agcccctcg aaagccgtc ccggcgcga atcgcctcc ttcgggcgt gaggtaata agcgcctgg aagcagaac gtgtacctg cactgcaag cgcgtcgga atccctctt ccgaacgca atttggcgc gtcaacgac ctacccctc gaagtcgga ggccgtaaa acgatcttc cccaacgcc acgcttgtg attaaagac actcaaagc gtggatcaa gccaactac tcgtgcagc gtcgaaaat caatacgga agagatgaa atcgaatac agtttgaag gttttagtg ccaccggaa gcgcccgtt ttaagcgtc gttgaagct ttcaccgat agcttacac ctgagatgg agcgatcag ggcaacggg ggatcaccg atactcgga tacgtgatc aattacaag cgagaccac ggggactgg gaggaattg caaatttcc gcaaggacc gacgagcac atgcttagg aatttgtgg tgtgggacc agatatctc ctctacatc acggccttc aacaggatt ggcacggga ttgccgtgc gatatcgtc ggagctcac acgaaagga acagtccct gtgaagccg aagcagtcg cagatgctg accatgaac gcgaccgtg gtgaccatc tggctggac tcgtggggc gacggcggc tgcggcatc ctctacttc gtgatagag tttcgcccc ggaaagcac tccccttgg atcatgtcg tccaaccac gtcaaaccc accgaacga atttacagc ataagcgaa ctatggcct gccaccgaa taccagtta aaaatcacc gctcacaac aacgccggc aacaccgaa gccctgtac aacttcacc acattaaca ctcttagct gttccagag ctggcaccg cccgtctcg cactcgggg gatcaccca ttctacacc aacgctcgg gtcctagtt cccatcatt ctgtcgctg ttggtgctc gtggcgctg atcgcgacg ctcttctgg cgcaaaaag attcggagc gagcgggag agccagaat cggtcgatg ggcgagtcg ccctccatg gcccagatc cagaataag cagaacaga gaccagcag tatctggct gtgcgggtg ggacgagga ccgcagcct ctggccatc gattcggac acgtataag gccgaatcg gcggattat atcgaggat atttgtccc tatgccacg tttcaattg tccaaacca gcgtacagt gaaagctcg tacagcgga aacgtgtac agtggaccg taccattcg gtacggggt tcttttgtt tatcatgat gttaaacca ccacctatt gataagttt aagttagga cataataaa gaaccagaa tataccaag gttcgaaga aaaggggga agactgaga gatccacac tcagagagt caagagtct gataacctg ggcagcact gactcagaa gtgaagaag atactaact ctgcacctg cctatttca gagtacgac accctcggt tcggattcc gagggcgac ggccgcgcc accagccaa gaactcatg tcttttagc catagaatg cgtggcggc gccgaaggc tcgtcctcc tcctcggag acgtcgccc ccgtccggc agcgtgggg cgcaagccc taccccgcg cgcaagggc aaggccaag ggcgccccc ttgagcaag cggcacgtg cgcagcagc agcgggtat tcgagtcac aacgacgag accaccttc agcatctcg cacgcgccg agcttcaac gaacggata catcccccg agcagattc tccgacttg aaccccaga gagctgagc gagagcgac tttgacaaa gggcacaag ccgagagga atgtccaaa ttgacacga gaagctttc caaatcaac gtttaa
- >TC033567 RA
actcgcagg aatagtcag tgtgtggcc gtcgaacgg gactcgtcc ttgtgtgtg atggtgttc tgatagtat ggggcagct atgagcagt gccccgatt gtatggtgc tggaccgcg ctcttcttc tcatcggca tggagtgga ggcttggac atgcaagga cctttgttc ttgcacgaa ccgccacac agagtggaa tttagcaac atttcgggc gcgaaagtg gactgcaca gcacatggg agtccttct ccggaggtg gagtggatt ctggcggat gggtcctca gtgcatcag gtgcctgaa ctccgactg ttattcccc aacgggtct ttggtgttt cccccgttt ccgaacgag aggtaccga cacgacgtc cacgcagcg ctctacagg tgcaaactg aaaagttca ctagggacg gttgtcagc cgggaagtg catgttaaa gctgttgtg aagcaaaaa tacgacgtc caagtccat gacgaatac gtgattgcg ggcaacaca gcggtcctc aaatgcaag attccgaat tacgtggct gaatacgtg atggtcacc tcctgggtc caagacgaa accatcaac atctacccc aacacggac attggcggc aaatacgtc gtcctcagc aacggggac ctctacata agtaatgca ggacctggg gacgggtac aagaactac ggctgtcgc actgttaac cggctcaca ggtgaagtc cagacgagc atgtatccg ggaaggata atcgtgacg gaaccgaag ggcaacgtc cagccacgg atcagcgtg gacaagtac aatgccagg agggtggtc ctgggggag gacgtcacg ctgccgtgc gtcgcgcaa ggacatcct gttcctgga tactactgg aagagggaa ctgcaagga caaagcgta cctgttgct ctgggggaa agactgacc attttatcg gcaggatta ctgaggata tccaaggtg cggttggag gaccgcggg gtgtacgtg tgcttcgcc aacaactct gcgggcgag gagagcgtg agggtgacg ctggaaata acagcgccc ttgtcagca catgttcaa cctcaagta caagtggtc gacgtcggg aaggaggcc agcttccag tgcattgtc aacggatat cccgtctct caagtcagc tggctgcac aacggcaag cccgtggcg cccgaccac cgcgtggag gtgatggcg gagccccca cgtctcacc attcgccaa ttatcgaaa gacgaccgc ggaatgtac cagtgcctg gcgagcaac aactgggac caagcccaa gccactgcc gaactggac atgggcgac gccggtcct gagctgatc tattggttc tcagagcag acgctccag ccaggaccg atggtttcg ctgaaatgt gtcgcaaca ggaaacccg ccgcctcag ttcacgtgg actctggat gggtttccg cttccagac catcctcga tttttggtc gggcaatat gtcacgatc caggacgac gtgatcagc cacgtgaat atcaccaat atcaaggcg gaagatggg ggcgaatac acatgtacg gcgcataac agcgtcggg aaaatatca cacagtgcc agggtgaac gtctacggc ctcccctac ataagagaa atgccgaaa atcaccgga gtggccgga ggcagtctg gtgatcaaa tgtcccgtg gcgggatac cccatcgag accataagc tgggagcgc gaaggccaa atcctgccg gtgaatcgt cggcagcgg gtttacgcc aacggcact ctggtggtg gagcagacg cagaggaac gaggacgcc ggcacctac acgtgccag gcgcaaaat cggcagcgg aactccgac agaaggaac gtcgaggtg caagttata gtaccgcca aagatcctg cccataaat ccgatgacg ggtgtgttg cgagaggga atgcgtgcg gcaataacc tgccaaatt atggaagga gacttgccg ataacattc cggtgggag cgcaacgcc aaagccgtt tccaataac gtcgctctg ggaacggta ataaggcga atcgacgag ttttcctcg tctttaatt atcgaacag gtgacatcc gctcattcc ggaaattac acttgcatc gccagcaac gtggcgggg gcggagaag cacgtcgtg ccgctcact gtcaacgtg ccgccgagg tggacggtg gagcccgca gactccagc gtcgctgct ggacaggag gccaccatg cattgccag gcagatggg tatcccaag ccagctgtc acctggaaa aaggccgtg ggtgagcaa cccggcgaa tacaaagac ttcctgttc gagcccaac gtgcagcaa ttccccaac ggttcgtta aacttcctc cacatatca aaagaaaac gaagggcaa tacttatgc gaagcgaaa aacaatatc ggcgctggc gtcagcaaa gtcattttc ctcaaagtc aacgctccg gcccacttc ctgcagaaa tcgaagcag ctgcaggtg gtgaagggc gagcaggcc cacctgcag tgctcggcc ctcggcgac acccccatg gagatcacc tggaagctc gggggccaa cacatatcc aacgacggc gatcagcgc tactccgtg cgggcgcag caactggcc gaggggatg gtctccgag ctgagcatt gagaggacg atcagacag gacacgggg atttttacg tgttcggcg agcaacgcc tacggaagc gatgacatg aatattcag ctgatcgtg caggagatt ccagaaccg ccgaggaac gtcagggta atggaccag ttatcgaga tcgatcggc atatcttgg acgcagccg tacgccgga aatagcccc atcactaac tacattgtc cagtacaaa ccgggatca gaagcgtgg ccgaatcag ccggccaag gtgacagtt cccggctcg caaatcacc acgactttg cagaacctg cgccctgcc caagtatat cacctccgg attttggcg gagaaccgc ttgggtcta agcgacccg agtcaagtc gtccaagtg agcaccctg gaagaagtg ccgagcggg gcccctgtt gatataagg gccgaggcg aagagttca actgaattg gtggtcact tgggaaccc ccgaatcgg gaaacttgg aatggtaat ctcttaggc tatcatgtg gggtaccag gaggtctcg acggaagat tcgaacaat gtggcgcag tcctacacc tttaaaagc gtggaagtg aggccacat tatgggggc gaagccgtg ctgcaaggc ctgtccaag tacaccacg tatgggatt atagtacag gcttacaac agccgagga tccggccct gctagcgac cccgtaaca gcgcgaact ctggaagat gcgccaagt ttgccccct ctgaacgtt cagtgttcg gtattaagt gctcaaagt ttacacata tcatgggaa ccgccgttg ccggaagga cgaaacgga attatccaa ggatataaa gttacttat cactcggcc ggcgaatgg ttcgacaac gacgatcaa cagaccaag atcatcaat caattgcgt acaacaatc tccggattg aggaaattc accaattac agcatgaca gtgctggcc tacacttcg agcggagac ggggcgagg tccgagccc acctactgc cagaccgag gaggatgtg ccatctgca ccggcggaa attaaggcc gtcctaagt gcccccgat aaaatacta gtatcgtgg ctgccaccc aaattcagt aatgggcca ttagttgga tacacgttc tacatgagc caaatggag gacggccga gaggaaggg acccacaaa agggtgctg agcccctcc accgaaatg cacgaagtt gagagggtt aaacaatcg tcaacgctc gagttctgg gtcacggcc tcaacaagg atcggcgaa ggcgagagc acacgtgtc atcaccgtg agcccctcg aaagccgtc ccggcgcga atcgcctcc ttcgggcgt gaggtaata agcgcctgg aagcagaac gtgtacctg cactgcaag cgcgtcgga atccctctt ccgaacgca atttggcgc gtcaacgac ctacccctc gaagtcgga ggccgtaaa acgatcttc cccaacgcc acgcttgtg attaaagac actcaaagc gtggatcaa gccaactac tcgtgcagc gtcgaaaat caatacgga agagatgaa atcgaatac agtttgaag gttttagtg ccaccggaa gcgcccgtt ttaagcgtc gttgaagct ttcaccgat agcttacac ctgagatgg agcgatcag ggcaacggg ggatcaccg atactcgga tacgtgatc aattacaag cgagaccac ggggactgg gaggaattg caaatttcc gcaaggacc gacgagcac atgcttagg aatttgtgg tgtgggacc agatatctc ctctacatc acggccttc aacaggatt ggcacggga ttgccgtgc gatatcgtc ggagctcac acgaaagga acagtccct gtgaagccg aagcagtcg cagatgctg accatgaac gcgaccgtg gtgaccatc tggctggac tcgtggggc gacggcggc tgcggcatc ctctacttc gtgatagag tttcgcccc ggaaagcac tccccttgg atcatgtcg tccaaccac gtcaaaccc accgaacga atttacagc ataagcgaa ctatggcct gccaccgaa taccagtta aaaatcacc gctcacaac aacgccggc aacaccgaa gccctgtac aacttcacc acattaaca ctcttaggt gctgttcca gagctggca ccgcccgtc tcgcactcg ggggatcac ccattctac accaacgct cgggtccta gttcccatc attctgtcg ctgttggtg ctcgtggcg ctgatcgcg acgctcttc tggcgcaaa aagattcgg agcgagcgg gagagccag aatcggtcg atgggcgag tcgccctcc atggcccag atccagaat aagcagaac agagaccag cagtatctg gctgtgcgg gtgggacga ggaccgcag cctctggcc atcgattcg gacacgtat aaggccgaa tcggcggat tatatcgag gatatttgt ccctatgcc acgtttcaa ttgtccaaa ccagcgtac agtgaaagc tcgtacagc ggaaacgtg tacagtgga ccgtaccat tcggtacgg ggttctttt gtttatcat gatgttaaa ccaccacct attgataag tttaagtta ggacataat aaagaacca gaatatacc aaggttcga agaaaaggg ggaagactg agagatcca cactcagag agtcaagag tctgataac ctgggcagc actgactca gaagtgaag aagatacta actctgcac ctgcctatt tcagagtac gacaccctc ggttcggat tccgagggc gacggccgc gccaccagc caagaactc atgtctttt agccataga atgcgtggc ggcgccgaa ggctcgtcc tcctcctcg gagacgtcg cccccgtcc ggcagcgtg gggcgcaag ccctacccc gcgcgcaag ggcaaggcc aagggcgcc cccttgagc aagcggcac gtgcgcagc agcagcggg tattcgagt cacaacgac gagaccacc ttcagcatc tcgcacgcg ccgagcttc aacgaacgg atacatccc ccgagcaga ttctccgac ttgaacccc agagagctg agcgagagc gactttgac aaagggcac aagccgaga ggaatgtcc aaattgaca cgagaagct ttccaaatc aacgtttaa gggccggaa ggcgccggg actgcacct tgtgacgat taatatata ctaattgtg gta
- >TC033567 RB
actcgcagg aatagtcag tgtgtggcc gtcgaacgg gactcgtcc ttgtgtgtg atggtgttc tgatagtat ggggcagct atgagcagt gccccgatt gtatggtgc tggaccgcg ctcttcttc tcatcggca tggagtgga ggcttggac atgcaagga cctttgttc ttgcacgaa ccgccacac agagtggaa tttagcaac atttcgggc gcgaaagtg gactgcaca gcacatggg agtccttct ccggaggtg gagtggatt ctggcggat gggtcctca gtgcatcag gtgcctgaa ctccgactg ttattcccc aacgggtct ttggtgttt cccccgttt ccgaacgag aggtaccga cacgacgtc cacgcagcg ctctacagg tgcaaactg aaaagttca ctagggacg gttgtcagc cgggaagtg catgttaaa gctgttgtg aagcaaaaa tacgacgtc caagtccat gacgaatac gtgattgcg ggcaacaca gcggtcctc aaatgcaag attccgaat tacgtggct gaatacgtg atggtcacc tcctgggtc caagacgaa accatcaac atctacccc aacacggac attggcggc aaatacgtc gtcctcagc aacggggac ctctacata agtaatgca ggacctggg gacgggtac aagaactac ggctgtcgc actgttaac cggctcaca ggtgaagtc cagacgagc atgtatccg ggaaggata atcgtgacg gaaccgaag ggcaacgtc cagccacgg atcagcgtg gacaagtac aatgccagg agggtggtc ctgggggag gacgtcacg ctgccgtgc gtcgcgcaa ggacatcct gttcctgga tactactgg aagagggaa ctgcaagga caaagcgta cctgttgct ctgggggaa agactgacc attttatcg gcaggatta ctgaggata tccaaggtg cggttggag gaccgcggg gtgtacgtg tgcttcgcc aacaactct gcgggcgag gagagcgtg agggtgacg ctggaaata acagcgccc ttgtcagca catgttcaa cctcaagta caagtggtc gacgtcggg aaggaggcc agcttccag tgcattgtc aacggatat cccgtctct caagtcagc tggctgcac aacggcaag cccgtggcg cccgaccac cgcgtggag gtgatggcg gagccccca cgtctcacc attcgccaa ttatcgaaa gacgaccgc ggaatgtac cagtgcctg gcgagcaac aactgggac caagcccaa gccactgcc gaactggac atgggcgac gccggtcct gagctgatc tattggttc tcagagcag acgctccag ccaggaccg atggtttcg ctgaaatgt gtcgcaaca ggaaacccg ccgcctcag ttcacgtgg actctggat gggtttccg cttccagac catcctcga tttttggtc gggcaatat gtcacgatc caggacgac gtgatcagc cacgtgaat atcaccaat atcaaggcg gaagatggg ggcgaatac acatgtacg gcgcataac agcgtcggg aaaatatca cacagtgcc agggtgaac gtctacggc ctcccctac ataagagaa atgccgaaa atcaccgga gtggccgga ggcagtctg gtgatcaaa tgtcccgtg gcgggatac cccatcgag accataagc tgggagcgc gaaggccaa atcctgccg gtgaatcgt cggcagcgg gtttacgcc aacggcact ctggtggtg gagcagacg cagaggaac gaggacgcc ggcacctac acgtgccag gcgcaaaat cggcagcgg aactccgac agaaggaac gtcgaggtg caagttata gtaccgcca aagatcctg cccataaat ccgatgacg ggtgtgttg cgagaggga atgcgtgcg gcaataacc tgccaaatt atggaagga gacttgccg ataacattc cggtgggag cgcaacgcc aaagccgtt tccaataac gtcgctctg ggaacggta ataaggcga atcgacgag ttttcctcg tctttaatt atcgaacag gtgacatcc gctcattcc ggaaattac acttgcatc gccagcaac gtggcgggg gcggagaag cacgtcgtg ccgctcact gtcaacgtg ccgccgagg tggacggtg gagcccgca gactccagc gtcgctgct ggacaggag gccaccatg cattgccag gcagatggg tatcccaag ccagctgtc acctggaaa aaggccgtg ggtgagcaa cccggcgaa tacaaagac ttcctgttc gagcccaac gtgcagcaa ttccccaac ggttcgtta aacttcctc cacatatca aaagaaaac gaagggcaa tacttatgc gaagcgaaa aacaatatc ggcgctggc gtcagcaaa gtcattttc ctcaaagtc aacgctccg gcccacttc ctgcagaaa tcgaagcag ctgcaggtg gtgaagggc gagcaggcc cacctgcag tgctcggcc ctcggcgac acccccatg gagatcacc tggaagctc gggggccaa cacatatcc aacgacggc gatcagcgc tactccgtg cgggcgcag caactggcc gaggggatg gtctccgag ctgagcatt gagaggacg atcagacag gacacgggg atttttacg tgttcggcg agcaacgcc tacggaagc gatgacatg aatattcag ctgatcgtg caggagatt ccagaaccg ccgaggaac gtcagggta atggaccag ttatcgaga tcgatcggc atatcttgg acgcagccg tacgccgga aatagcccc atcactaac tacattgtc cagtacaaa ccgggatca gaagcgtgg ccgaatcag ccggccaag gtgacagtt cccggctcg caaatcacc acgactttg cagaacctg cgccctgcc caagtatat cacctccgg attttggcg gagaaccgc ttgggtcta agcgacccg agtcaagtc gtccaagtg agcaccctg gaagaagtg ccgagcggg gcccctgtt gatataagg gccgaggcg aagagttca actgaattg gtggtcact tgggaaccc ccgaatcgg gaaacttgg aatggtaat ctcttaggc tatcatgtg gggtaccag gaggtctcg acggaagat tcgaacaat gtggcgcag tcctacacc tttaaaagc gtggaagtg aggccacat tatgggggc gaagccgtg ctgcaaggc ctgtccaag tacaccacg tatgggatt atagtacag gcttacaac agccgagga tccggccct gctagcgac cccgtaaca gcgcgaact ctggaagat gcgccaagt ttgccccct ctgaacgtt cagtgttcg gtattaagt gctcaaagt ttacacata tcatgggaa ccgccgttg ccggaagga cgaaacgga attatccaa ggatataaa gttacttat cactcggcc ggcgaatgg ttcgacaac gacgatcaa cagaccaag atcatcaat caattgcgt acaacaatc tccggattg aggaaattc accaattac agcatgaca gtgctggcc tacacttcg agcggagac ggggcgagg tccgagccc acctactgc cagaccgag gaggatgtg ccatctgca ccggcggaa attaaggcc gtcctaagt gcccccgat aaaatacta gtatcgtgg ctgccaccc aaattcagt aatgggcca ttagttgga tacacgttc tacatgagc caaatggag gacggccga gaggaaggg acccacaaa agggtgctg agcccctcc accgaaatg cacgaagtt gagagggtt aaacaatcg tcaacgctc gagttctgg gtcacggcc tcaacaagg atcggcgaa ggcgagagc acacgtgtc atcaccgtg agcccctcg aaagccgtc ccggcgcga atcgcctcc ttcgggcgt gaggtaata agcgcctgg aagcagaac gtgtacctg cactgcaag cgcgtcgga atccctctt ccgaacgca atttggcgc gtcaacgac ctacccctc gaagtcgga ggccgtaaa acgatcttc cccaacgcc acgcttgtg attaaagac actcaaagc gtggatcaa gccaactac tcgtgcagc gtcgaaaat caatacgga agagatgaa atcgaatac agtttgaag gttttagtg ccaccggaa gcgcccgtt ttaagcgtc gttgaagct ttcaccgat agcttacac ctgagatgg agcgatcag ggcaacggg ggatcaccg atactcgga tacgtgatc aattacaag cgagaccac ggggactgg gaggaattg caaatttcc gcaaggacc gacgagcac atgcttagg aatttgtgg tgtgggacc agatatctc ctctacatc acggccttc aacaggatt ggcacggga ttgccgtgc gatatcgtc ggagctcac acgaaagga acagtccct gtgaagccg aagcagtcg cagatgctg accatgaac gcgaccgtg gtgaccatc tggctggac tcgtggggc gacggcggc tgcggcatc ctctacttc gtgatagag tttcgcccc ggaaagcac tccccttgg atcatgtcg tccaaccac gtcaaaccc accgaacga atttacagc ataagcgaa ctatggcct gccaccgaa taccagtta aaaatcacc gctcacaac aacgccggc aacaccgaa gccctgtac aacttcacc acattaaca ctcttagct gttccagag ctggcaccg cccgtctcg cactcgggg gatcaccca ttctacacc aacgctcgg gtcctagtt cccatcatt ctgtcgctg ttggtgctc gtggcgctg atcgcgacg ctcttctgg cgcaaaaag attcggagc gagcgggag agccagaat cggtcgatg ggcgagtcg ccctccatg gcccagatc cagaataag cagaacaga gaccagcag tatctggct gtgcgggtg ggacgagga ccgcagcct ctggccatc gattcggac acgtataag gccgaatcg gcggattat atcgaggat atttgtccc tatgccacg tttcaattg tccaaacca gcgtacagt gaaagctcg tacagcgga aacgtgtac agtggaccg taccattcg gtacggggt tcttttgtt tatcatgat gttaaacca ccacctatt gataagttt aagttagga cataataaa gaaccagaa tataccaag gttcgaaga aaaggggga agactgaga gatccacac tcagagagt caagagtct gataacctg ggcagcact gactcagaa gtgaagaag atactaact ctgcacctg cctatttca gagtacgac accctcggt tcggattcc gagggcgac ggccgcgcc accagccaa gaactcatg tcttttagc catagaatg cgtggcggc gccgaaggc tcgtcctcc tcctcggag acgtcgccc ccgtccggc agcgtgggg cgcaagccc taccccgcg cgcaagggc aaggccaag ggcgccccc ttgagcaag cggcacgtg cgcagcagc agcgggtat tcgagtcac aacgacgag accaccttc agcatctcg cacgcgccg agcttcaac gaacggata catcccccg agcagattc tccgacttg aaccccaga gagctgagc gagagcgac tttgacaaa gggcacaag ccgagagga atgtccaaa ttgacacga gaagctttc caaatcaac gtttaaggg ccggaaggc gccgggact gcaccttgt gacgattaa tatatacta attgtggta
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