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Gene information
General information
TC033721
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008989
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9209379..9215442
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033721
    iBeetle screen iB_06261

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033721 RA
      MAGLEQLAT VDQLIMRME TESSETRHK 
      IFVENSAGK KLFYPGTVS GRFRRNLYL 
      SLQAFNLKI LDNLKNEVI HVHRPGLCC 
      SLSCGLSLD LFILAPPDT FVGKIEEKC 
      RICGTKFGI KDASGKKVF VIKGPCCVR 
      CSKNVELKI LAADEKTQV GFISKQAKA 
      KCLRMSFPK DMDVEMKAV MVGACFLIF 
      ATFFNVNMS RLWQYHFS
    CDS sequence
    • >TC033721 RA
      atggcaggc ttggaacag cttgctacg gttgaccag 
      ttgataatg cgtatggag accgaatca tctgagacc 
      cggcataag atttttgtg gagaatagt gccggcaaa 
      aaactcttc taccctggc accgtctcc ggtcgtttc 
      agaagaaac ctttacctc tcactgcaa gctttcaac 
      ctgaaaatt ttagataat ctcaaaaac gaagtgatt 
      catgtgcat cgtcctgga ctgtgttgt tcgctcagc 
      tgtggcttg tccctagac ctatttatt ttagcacct 
      cctgataca tttgtggga aaaattgaa gaaaaatgc 
      aggatttgt ggcaccaaa ttcgggatt aaagatgca 
      agtgggaaa aaggtgttt gtaataaaa ggaccatgt 
      tgtgttcgc tgttctaaa aatgtcgaa ctaaaaata 
      ctggcggcg gatgagaag acacaagtg gggttcatt 
      tccaagcaa gcgaaggct aagtgtctt aggatgtcc 
      tttcctaag gatatggat gtagaaatg aaagctgtg 
      atggtaggg gcttgcttt ttaattttt gccactttc 
      tttaacgta aatatgagt cgattatgg caatatcat 
      ttttcttaa
    mRNA sequence
    • >TC033721 RA
      acaggccgc tcaaaactg gcgcaccaa ctcagtgtg 
      tcatttaaa atttaagtt taaagtttt attattaaa 
      accagtatg gcaggcctg ggacagctt gcaaagctt 
      gatcaactg atactgcgt ctggacgac agatcgtct 
      ggaactcgg tataaaatt tctgtggaa aatagtgca 
      ggccaagaa ctctttacc cctggcaag gtctcgggt 
      cgttttagc cgaaacgtt tacctctca ttgaatgat 
      ttcaaccta gaaatctta gataattcc aaaagcgaa 
      gtgattcat cttcaccgt cctgcatta tcgtgctgt 
      tctatggga ggggacata aacctaaag gtttatgca 
      cctccacgc acatttgtg ggagaaatt aaagaaaag 
      tgtagttta tgtggcact caattttgg attaaagat 
      gcatttaac aataaggtg tttttgata aagggaccg 
      acttgtttt tgctgtact agtggactc aaatttaaa 
      atattgtcg gctggtgac ggtacacag gtgggtttg 
      attgccaaa gaagcagac actaaagca cttagaata 
      tctttcccc aaggatttg gatgtacaa atgaaagct 
      gtaatgtta ggagccagc tttttaatt tttgctgtc 
      ttcttcaac gtaagcata gatcagtta taccaatat 
      tactatcct gacccgttt gtttacgtt taataataa 
      atgcatatt tattttttg ttcaattat tattagtga 
      gtacctata ttttttatc cttttttga taaatattt 
      gattttaaa attacaatt tcggcaata gcacctaag 
      gataaaccc tttgtgttc aatatgagt ttcagttgg 
      tatttctcc tgggttaag gacctttta aaatccaga 
      ttttatcaa ggaccttat ctatacaaa taaacacaa 
      actcaagaa cctaattac gagaatctt taatttaag 
      aaggtcaag cacgaaata aaaccaaaa ttctaattc 
      aaaaatgat aactgctga atgcagctg aatagagtc 
      ctgacaaat gtcactacg acagtgggg caaattgaa 
      ctaattttt ctcatttgg agtgggtgg acaagatat 
      ctgtcaatt ctcacaacc ccaaatatt tttgaaatg 
      tcatcactt aaatattaa gaactgcat ttttaaaat 
      cgaaggtcc tgccattat ggcaggctt ggaacagct 
      tgctacggt tgaccagtt gataatgcg tatggagac 
      cgaatcatc tgagacccg gcataagat ttttgtgga 
      gaatagtgc cggcaaaaa actcttcta ccctggcac 
      cgtctccgg tcgtttcag aagaaacct ttacctctc 
      actgcaagc tttcaacct gaaaatttt agataatct 
      caaaaacga agtgattca tgtgcatcg tcctggact 
      gtgttgttc gctcagctg tggcttgtc cctagacct 
      atttatttt agcacctcc tgatacatt tgtgggaaa 
      aattgaaga aaaatgcag gatttgtgg caccaaatt 
      cgggattaa agatgcaag tgggaaaaa ggtgtttgt 
      aataaaagg accatgttg tgttcgctg ttctaaaaa 
      tgtcgaact aaaaatact ggcggcgga tgagaagac 
      acaagtggg gttcatttc caagcaagc gaaggctaa 
      gtgtcttag gatgtcctt tcctaagga tatggatgt 
      agaaatgaa agctgtgat ggtaggggc ttgcttttt 
      aatttttgc cactttctt taacgtaaa tatgagtcg 
      attatggca atatcattt ttcttaagg tttttaata 
      aacgcattt ttatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY