Status
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Gene information
General information
TC008819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10391349..10396596
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008819
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06260

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    egg chamber mostly not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008819 RA
      MDRSFNSSF STRSPFSPR SRQNLNAKK IIPPNFRKS 
      SRFSVSGKS TQSVQIILS TPYNYVERF GQTFPVLIT 
      EALTFADRN TVVSARISK LGYLWVVCG RRLLIWQYR 
      QSPVLLDTP SKKTSLLNQ CFELQLPQS DLAHRAELV 
      SVFVNSDSN TPSCIAVSP EGVVRYWPA IVHEGISVE 
      QHVSLQGQE CDSLTDVEG LGCILATTT CTVLLLQPQ 
      LSGGRHSLK CKTLKIPTG WLGGISKRM SSLIFGPIA 
      SETSAESGI VRVLSVPGT TSDTWTVYV LADHNLQKW 
      QLSKNDEEL IFVTELNRI VRDSFCGAL WENCVGDQT 
      EIDTWLLDI QSDKDNVII LAAAVNIPR VHYAMISLQ 
      TNTNQPPTA VKDFLLLKI TDLYRENNP GDSLSYRFL 
      LCGINAYLY NQKHITVIK PQEEPDILE FNTPQDFLL 
      AGAICVNTP IFFSRNHGL VAVASNDMS NDLNLSLTG 
      ANSFVETSF NDNVVVNNL SIYNMDPEE ISIAYKDTV 
      SQLKAAFIY RIQNQEQAC KEIIDELFP PDASKLPGI 
      DSLLDKVVI MMGKDLIDD TPAGDPRWS KDHQVGLGS 
      SHSMQVLDQ LKNKQKALS LFIKFLKES GLWAQLSAS 
      TIRNTTTAT TYIMGEISE KIIAAISLK TVAYNQVLE 
      KAIEKATAE SETKPENGL TYQDVFFRE VSQVHRGIQ 
      EMVNYCEDK SHSNMDPAQ IVDMIHDTN EIILGALKS 
      VINFREQNV VNYKPSEGV LAIAPEFLP WTAASGAEG 
      LFNSLSQMH YLTYNYGII LTNNERLRN DLFDQLVSI 
      TDIILDGRK SHLESIKAT SFEEIIYKQ YCSDRQKFM 
      KPFIHEKDW ERCAKLAEK YLDFETLMI LCEATNNQR 
      RLDDYLERF SNEGFSEFV YNWFLKENK QGKLIDMCK 
      HVGKSKNIQ KLTNFLSGH PSLSWMQEI FDKKYNQAA 
      NSLQTLALN ETESVTRQK TMLSLCKLA KLAAGTGSE 
      EALRSVDSR LDLIAYQED IPDYVLQQF GYDTINIRV 
      IPPKDLINL YICKEYTEA AELEFKKAL DLLNFIDDE 
      ELRQEMSLK IWRYALLRD AWQHQNLDS PMEILQHKL 
      FFKLVDLAM DLEGDGWNL LPPMDTLID DDSLGDLRN 
      NTNFQFLLK AGYEHIYRS QTVE
    CDS sequence
    • >TC008819 RA
      atggataga agcttcaac tcatctttc tccaccaga 
      agcccgttt tctccccgt tctcggcaa aatctcaac 
      gccaagaaa atcattccg cctaatttc cgaaaatca 
      tcccgtttc agtgtctca ggaaaatca acccaatcg 
      gtacaaata atactatca acaccttac aactacgtt 
      gaacgtttc ggccaaacc ttccccgtc ctaataaca 
      gaagccctc acatttgcc gacagaaac accgtggta 
      tcggcccga atctcgaaa ctgggctac ctgtgggtg 
      gtctgcggc cgccgcttg ctgatctgg cagtaccgc 
      caaagcccc gtgttgctc gacaccccc tcgaaaaag 
      acaagttta ctaaaccag tgctttgaa ctgcaactc 
      ccgcaaagc gacttggcg cacagagct gaactggtc 
      tcggttttc gtcaattca gactcgaac acaccttct 
      tgtatcgca gtctcgccg gaaggtgtg gtgcgctac 
      tggccggca attgtccat gaaggaatc tccgtggaa 
      cagcacgtc agtctacaa ggccaggaa tgcgacagt 
      ttgaccgat gtcgagggc cttgggtgc attctggcc 
      accacaacg tgcaccgtt ttgttgctc cagccacag 
      ctaagtggc gggaggcac agtttgaaa tgcaaaaca 
      cttaaaata ccaactggg tggttgggg ggcattagc 
      aagagaatg tcgagtctg atttttggg ccaatcgcg 
      agcgaaacc tcggccgaa agtggaata gtgcgcgtt 
      ctgagcgtc cctggtaca actagcgac acctggacg 
      gtgtacgtt ttagccgac cacaatttg caaaagtgg 
      caattgtcc aaaaacgac gaagagttg atatttgtc 
      accgaattg aaccgaata gtcagagat agtttctgt 
      ggtgccctg tgggagaat tgtgtcgga gaccaaacc 
      gaaattgat acctggtta cttgacata cagtcagat 
      aaagacaat gttataata ctcgcagca gcggttaat 
      attcctcgg gtacattac gcgatgata agtctacag 
      acgaacaca aatcaacct ccaacagcc gttaaagat 
      tttcttttg ttgaaaatt acggacttg tatcgggaa 
      aacaatccg ggggattcc ctatcgtat cgttttctt 
      ttatgcggc attaatgcc tacttatac aaccagaaa 
      catatcacc gttatcaag ccccaagaa gagcctgat 
      attttagag ttcaacaca cctcaagat tttttatta 
      gcgggtgcg atctgtgtc aacacacct atatttttc 
      tcgcgcaat catggctta gtcgcagtg gcgagcaat 
      gatatgtct aatgactta aacctaagt ctcacaggc 
      gctaacagt ttcgtggag acgtcgttt aatgataac 
      gtggtggtt aataacctg tccatttac aacatggac 
      cctgaggag atttccatt gcttacaaa gacacagtt 
      agtcaatta aaggccgct tttatctac cggatacaa 
      aaccaggag caagcctgc aaggaaatc atcgacgag 
      ttgttccct cctgacgcg tccaaactc ccaggaatc 
      gactcatta ctggataaa gtcgtaata atgatgggc 
      aaggactta atcgatgac accccagcc ggtgatccc 
      agatggtca aaggaccat caagtcggg cttggcagc 
      tcacactcg atgcaagtc ctcgaccaa ttaaaaaac 
      aaacaaaaa gcactctca ctattcata aaattcctc 
      aaagagtca ggactgtgg gcgcaattg tccgcatcc 
      acaattcgc aacaccacc accgcaaca acgtacatc 
      atgggcgaa atatcggag aaaatcata gccgcgatc 
      agtctcaaa actgtcgcc tataatcaa gttctggaa 
      aaagccatc gaaaaagcg acggctgag tcggaaacc 
      aagccggaa aacgggcta acgtaccag gatgtgttt 
      ttccgggag gtcagtcag gtgcataga ggcattcaa 
      gagatggtg aattattgc gaggacaag tcacattcg 
      aacatggat ccggcccaa attgttgac atgatccac 
      gacaccaat gagattatt ttgggtgcg ctgaaaagc 
      gtgatcaac tttagggaa cagaatgtg gtgaattac 
      aaaccgtcg gagggtgtc cttgccata gcccccgaa 
      tttttgccc tggactgcg gccagtggg gccgaaggc 
      ctctttaat tctctgtct caaatgcat tatctcaca 
      tacaactac gggataatt ttaacgaac aatgagcgc 
      ttgaggaac gatcttttc gaccagttg gtctccatc 
      acggatata attctcgac ggccgcaaa agccacctg 
      gagagcatc aaagcgacg tcctttgag gaaattatt 
      tacaagcaa tactgttcc gaccgccag aaattcatg 
      aagcctttt atacacgaa aaagactgg gagagatgt 
      gccaaactt gccgaaaag tacctcgat tttgaaaca 
      ctgatgatc ctgtgtgaa gctacaaat aaccagcgc 
      cggctcgac gattatttg gaacgcttc agcaacgag 
      gggttctcc gaattcgtg tacaactgg tttttgaaa 
      gagaataaa caaggcaag ctgattgac atgtgcaag 
      cacgtcggc aagtcgaaa aatattcaa aaactcacc 
      aatttcctg tcgggtcat ccgtcactg tcgtggatg 
      caggagatt tttgataag aagtacaat caagcggcg 
      aactcgttg caaaccttg gctttgaac gagactgag 
      tcggtgaca aggcaaaaa acaatgttg agtttgtgc 
      aaactggcg aaattggcg gccgggacc ggcagtgag 
      gaggcattg aggagtgtt gattcgagg ctggacttg 
      attgcgtat caggaggat atcccggat tatgttttg 
      cagcagttt ggatacgac acgattaac attcgggtg 
      attccgccc aaagacttg attaatctt tacatttgt 
      aaagaatac acagaagcg gcggaattg gaatttaag 
      aaagccctc gatttgctg aatttcatc gacgatgag 
      gagttgaga caagaaatg tcgctgaaa atttggaga 
      tacgcgttg ttgagagac gcatggcaa caccaaaat 
      ttggatagt cctatggaa atcctacag cacaagctg 
      tttttcaaa ttggtcgat ctcgccatg gacctggaa 
      ggagacggg tggaacctt ctacccccc atggacacg 
      ctaatagac gacgactcg ctcggggac ttgcgaaat 
      aataccaac ttccagttt ttgttaaaa gcaggttac 
      gaacatatt tataggtca caaaccgta gagtag
    mRNA sequence
    • >TC008819 RA
      cataagtgg tcaacattg ttattactt tttaaattt 
      gaatatttg taataaagt cagaatgga tagaagctt 
      caactcatc tttctccac cagaagccc gttttctcc 
      ccgttctcg gcaaaatct caacgccaa gaaaatcat 
      tccgcctaa tttccgaaa atcatcccg tttcagtgt 
      ctcaggaaa atcaaccca atcggtaca aataatact 
      atcaacacc ttacaacta cgttgaacg tttcggcca 
      aaccttccc cgtcctaat aacagaagc cctcacatt 
      tgccgacag aaacaccgt ggtatcggc ccgaatctc 
      gaaactggg ctacctgtg ggtggtctg cggccgccg 
      cttgctgat ctggcagta ccgccaaag ccccgtgtt 
      gctcgacac cccctcgaa aaagacaag tttactaaa 
      ccagtgctt tgaactgca actcccgca aagcgactt 
      ggcgcacag agctgaact ggtctcggt tttcgtcaa 
      ttcagactc gaacacacc ttcttgtat cgcagtctc 
      gccggaagg tgtggtgcg ctactggcc ggcaattgt 
      ccatgaagg aatctccgt ggaacagca cgtcagtct 
      acaaggcca ggaatgcga cagtttgac cgatgtcga 
      gggccttgg gtgcattct ggccaccac aacgtgcac 
      cgttttgtt gctccagcc acagctaag tggcgggag 
      gcacagttt gaaatgcaa aacacttaa aataccaac 
      tgggtggtt ggggggcat tagcaagag aatgtcgag 
      tctgatttt tgggccaat cgcgagcga aacctcggc 
      cgaaagtgg aatagtgcg cgttctgag cgtccctgg 
      tacaactag cgacacctg gacggtgta cgttttagc 
      cgaccacaa tttgcaaaa gtggcaatt gtccaaaaa 
      cgacgaaga gttgatatt tgtcaccga attgaaccg 
      aatagtcag agatagttt ctgtggtgc cctgtggga 
      gaattgtgt cggagacca aaccgaaat tgatacctg 
      gttacttga catacagtc agataaaga caatgttat 
      aatactcgc agcagcggt taatattcc tcgggtaca 
      ttacgcgat gataagtct acagacgaa cacaaatca 
      acctccaac agccgttaa agattttct tttgttgaa 
      aattacgga cttgtatcg ggaaaacaa tccggggga 
      ttccctatc gtatcgttt tcttttatg cggcattaa 
      tgcctactt atacaacca gaaacatat caccgttat 
      caagcccca agaagagcc tgatatttt agagttcaa 
      cacacctca agatttttt attagcggg tgcgatctg 
      tgtcaacac acctatatt tttctcgcg caatcatgg 
      cttagtcgc agtggcgag caatgatat gtctaatga 
      cttaaacct aagtctcac aggcgctaa cagtttcgt 
      ggagacgtc gtttaatga taacgtggt ggttaataa 
      cctgtccat ttacaacat ggaccctga ggagatttc 
      cattgctta caaagacac agttagtca attaaaggc 
      cgcttttat ctaccggat acaaaacca ggagcaagc 
      ctgcaagga aatcatcga cgagttgtt ccctcctga 
      cgcgtccaa actcccagg aatcgactc attactgga 
      taaagtcgt aataatgat gggcaagga cttaatcga 
      tgacacccc agccggtga tcccagatg gtcaaagga 
      ccatcaagt cgggcttgg cagctcaca ctcgatgca 
      agtcctcga ccaattaaa aaacaaaca aaaagcact 
      ctcactatt cataaaatt cctcaaaga gtcaggact 
      gtgggcgca attgtccgc atccacaat tcgcaacac 
      caccaccgc aacaacgta catcatggg cgaaatatc 
      ggagaaaat catagccgc gatcagtct caaaactgt 
      cgcctataa tcaagttct ggaaaaagc catcgaaaa 
      agcgacggc tgagtcgga aaccaagcc ggaaaacgg 
      gctaacgta ccaggatgt gtttttccg ggaggtcag 
      tcaggtgca tagaggcat tcaagagat ggtgaatta 
      ttgcgagga caagtcaca ttcgaacat ggatccggc 
      ccaaattgt tgacatgat ccacgacac caatgagat 
      tattttggg tgcgctgaa aagcgtgat caactttag 
      ggaacagaa tgtggtgaa ttacaaacc gtcggaggg 
      tgtccttgc catagcccc cgaattttt gccctggac 
      tgcggccag tggggccga aggcctctt taattctct 
      gtctcaaat gcattatct cacatacaa ctacgggat 
      aattttaac gaacaatga gcgcttgag gaacgatct 
      tttcgacca gttggtctc catcacgga tataattct 
      cgacggccg caaaagcca cctggagag catcaaagc 
      gacgtcctt tgaggaaat tatttacaa gcaatactg 
      ttccgaccg ccagaaatt catgaagcc ttttataca 
      cgaaaaaga ctgggagag atgtgccaa acttgccga 
      aaagtacct cgattttga aacactgat gatcctgtg 
      tgaagctac aaataacca gcgccggct cgacgatta 
      tttggaacg cttcagcaa cgaggggtt ctccgaatt 
      cgtgtacaa ctggttttt gaaagagaa taaacaagg 
      caagctgat tgacatgtg caagcacgt cggcaagtc 
      gaaaaatat tcaaaaact caccaattt cctgtcggg 
      tcatccgtc actgtcgtg gatgcagga gatttttga 
      taagaagta caatcaagc ggcgaactc gttgcaaac 
      cttggcttt gaacgagac tgagtcggt gacaaggca 
      aaaaacaat gttgagttt gtgcaaact ggcgaaatt 
      ggcggccgg gaccggcag tgaggaggc attgaggag 
      tgttgattc gaggctgga cttgattgc gtatcagga 
      ggatatccc ggattatgt tttgcagca gtttggata 
      cgacacgat taacattcg ggtgattcc gcccaaaga 
      cttgattaa tctttacat ttgtaaaga atacacaga 
      agcggcgga attggaatt taagaaagc cctcgattt 
      gctgaattt catcgacga tgaggagtt gagacaaga 
      aatgtcgct gaaaatttg gagatacgc gttgttgag 
      agacgcatg gcaacacca aaatttgga tagtcctat 
      ggaaatcct acagcacaa gctgttttt caaattggt 
      cgatctcgc catggacct ggaaggaga cgggtggaa 
      ccttctacc ccccatgga cacgctaat agacgacga 
      ctcgctcgg ggacttgcg aaataatac caacttcca 
      gtttttgtt aaaagcagg ttacgaaca tatttatag 
      gtcacaaac cgtagagta ggtttttta cagtaaatt 
      gttttatac gaagtttta tttttctat tactaacga 
      caaaaaaag ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY