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Gene information
General information
TC008703 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14372248..14374162
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008703
      iBeetle screen iB_06257

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008703 RA
        MDLYNEVEL NSISSKDIE GNVDTDNQN NSKDDNSKL 
        EAIGPRGET EPARKRALE NELPDTLLV ENEDISTKK 
        RKLDDDSGK DQDEAIVCV DIRQDLNNS DPLPETIEP 
        FGDDSVESK LIPGQLSKN YESSEITNI TTVKSGVNS 
        EQGEESHGA SSKVVEIIE SEKAEGEQS LKISNEAVE 
        RKKHEVKEE LSHIFTDEP ENQNEIIGV EEIKLDSFE 
        KSTPDPDLC SKILALPVA SNWEGTSHK PYQLILVST 
        STPEYATVE HLFSSTSGR RGLNITKLE RVENPYLLG 
        QYLLKKEKM LAEAKSVTE RQLFHGTRE ANIDGICKF 
        GYDWWRPAQ RYGKSFGHK FGKGVSFTP EASYAYHYG 
        DNVMLLNKV LVSQSVIGD SSMTVPPES YDTSENSRG 
        TVIVKYDDD DFFPQYVIH YD
      CDS sequence
      • >TC008703 RA
        atggatctt tacaatgaa gtcgaactt aattccatt 
        tcatctaaa gacattgaa ggaaatgta gatacggac 
        aaccaaaac aatagtaaa gacgataat tcgaaacta 
        gaagcaatt ggacctcga ggagaaact gagcctgca 
        agaaaacgg gcattggaa aatgaactt cctgatacg 
        cttttagta gaaaatgaa gacataagc acaaaaaag 
        agaaaattg gatgacgac tctggtaaa gaccaagat 
        gaagcaatt gtatgtgtg gatattagg caagatttg 
        aacaattct gaccctctt cctgaaact attgaacct 
        ttcggagat gattctgta gaatctaaa ttaatccct 
        ggacaactt tcgaaaaat tacgaatcg tcagagatt 
        actaacata accacagtc aagtctgga gttaattct 
        gaacaagga gaggaatct catggagcg tcttccaag 
        gttgtagaa atcatcgaa tctgagaaa gcagaagga 
        gagcaaagt ttgaaaatt tcgaacgaa gcagttgaa 
        aggaagaaa cacgaagtc aaagaagaa ttaagtcat 
        atttttaca gacgaacca gaaaatcaa aacgaaata 
        atcggggtt gaagaaatc aagttagac agtttcgaa 
        aaatcgacg ccagatccg gacttgtgt tcaaaaatt 
        ctggcactg cctgtagca tccaactgg gaagggaca 
        tcacacaag ccgtaccaa ctgatactt gtcagtact 
        tcaacgcct gaatacgcc actgttgaa catttattt 
        tcatcaaca agtggaaga cgcggtttg aacataacc 
        aagttggaa cgcgttgaa aacccgtat ttgctgggc 
        cagtacttg ttgaaaaag gagaaaatg ttagcggag 
        gcaaaaagt gttacggaa agacagctc tttcatgga 
        actagagaa gcaaatatc gacggtatt tgcaagttc 
        ggttacgac tggtggcga cctgctcaa cgttacggg 
        aaatcattt ggacacaag tttggaaag ggagtttca 
        tttacaccg gaagcgtca tacgcgtac cattatggc 
        gacaatgtg atgctgctg aacaaggtg ttagtgtct 
        cagtcggtc attggagac tcctccatg acagtgcca 
        cccgaatct tacgacacc agtgaaaac tcaaggggt 
        acggttatt gtcaaatac gatgatgac gattttttc 
        cctcaatat gtcatacat tatgattaa
      mRNA sequence
      • >TC008703 RA
        cttttttaa agctactgg gattacatt ttacactta 
        cctttctgt aatagctga aattatcgc agaaaacat 
        tgttccatt gggaaaaat aacaaacca gaacagttc 
        aaatatgga tctttacaa tgaagtcga acttaattc 
        catttcatc taaagacat tgaaggaaa tgtagatac 
        ggacaacca aaacaatag taaagacga taattcgaa 
        actagaagc aattggacc tcgaggaga aactgagcc 
        tgcaagaaa acgggcatt ggaaaatga acttcctga 
        tacgctttt agtagaaaa tgaagacat aagcacaaa 
        aaagagaaa attggatga cgactctgg taaagacca 
        agatgaagc aattgtatg tgtggatat taggcaaga 
        tttgaacaa ttctgaccc tcttcctga aactattga 
        acctttcgg agatgattc tgtagaatc taaattaat 
        ccctggaca actttcgaa aaattacga atcgtcaga 
        gattactaa cataaccac agtcaagtc tggagttaa 
        ttctgaaca aggagagga atctcatgg agcgtcttc 
        caaggttgt agaaatcat cgaatctga gaaagcaga 
        aggagagca aagtttgaa aatttcgaa cgaagcagt 
        tgaaaggaa gaaacacga agtcaaaga agaattaag 
        tcatatttt tacagacga accagaaaa tcaaaacga 
        aataatcgg ggttgaaga aatcaagtt agacagttt 
        cgaaaaatc gacgccaga tccggactt gtgttcaaa 
        aattctggc actgcctgt agcatccaa ctgggaagg 
        gacatcaca caagccgta ccaactgat acttgtcag 
        tacttcaac gcctgaata cgccactgt tgaacattt 
        attttcatc aacaagtgg aagacgcgg tttgaacat 
        aaccaagtt ggaacgcgt tgaaaaccc gtatttgct 
        gggccagta cttgttgaa aaaggagaa aatgttagc 
        ggaggcaaa aagtgttac ggaaagaca gctctttca 
        tggaactag agaagcaaa tatcgacgg tatttgcaa 
        gttcggtta cgactggtg gcgacctgc tcaacgtta 
        cgggaaatc atttggaca caagtttgg aaagggagt 
        ttcatttac accggaagc gtcatacgc gtaccatta 
        tggcgacaa tgtgatgct gctgaacaa ggtgttagt 
        gtctcagtc ggtcattgg agactcctc catgacagt 
        gccacccga atcttacga caccagtga aaactcaag 
        gggtacggt tattgtcaa atacgatga tgacgattt 
        tttccctca atatgtcat acattatga ttaaaaaaa 
        catatttgt tttacgtgt aattttatt ttaaagtta 
        aataaaatt agtaaacac tttatctat aaatacaat 
        attatttat tcaggctgg cttaaaagc agtttttac 
        cccttcctt ggttacctc ccaattagc aaagtgtgg 
        ctttgtaca actgtcaaa taattaaac atttcaaaa 
        cgtttttgg gttaaaaaa tacgtagac tatgtacag 
        gtaataaaa aatatgggg tttatccgt aatgtctgc 
        atatttaat gaacacaaa tttttacta cacgcgcaa 
        gaaatatat gtttttctt gttcttttt ccttcaaaa 
        tttaaacaa taaatatgt tttaaatcg taatatttg 
        tcaggacag ttgcgatca aaagaatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY