Status
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Gene information
General information
TC008519 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12099447..12115078
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008519
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06256

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008519 RA
      MFCTKLLFS PKHDQLQHV FKHSSFHRP SVRVKKFIP 
      NPDKSRIKK TIPPSPCTF EVCVGPRVN QFNIQMLSP 
      HLFEQVFKG LSVNKCDEA IVKECLKTL QEHNMVQKP 
      ENFLNDVEL KIPPLEGKN IEEHFHLIG DQQVKPYRD 
      LVEKLLTNK IPKPPDKWV MQEGWTRYV PGEEPQQVP 
      YPLEEGLIF DIEVAVTVG KAPTLATAV SNEAWYGWV 
      SQMLLDGTS KPVTTHQYN LDQLIPLES TTKDTGAKL 
      TNHMKKPKI IIGHNVSYD RARVKEQYW LNQTATRFL 
      DTMSLHVCV SGLTSYQRA ILKSGKIDE EDEIWKQHS 
      SLNSLNEVH KLYCGGKSV AKETRDLFV TGSLNDIRE 
      EFQTVMKYC SGDVVATYS VLNELFPMF LERFPHPVT 
      LAGMLELST GYLPINSNW NRYIDNSEQ AYEDMDIEG 
      RLLLAKRAE QACQLLHDD KYKEDLWLW DEDWTVKNL 
      KIKKTSPKT KQIETPPPQ SPDDEATDP LEEKFRDLM 
      ATKANISRV NSHLPGYPT WYRKLSTKP DSPDWVPGP 
      HLISTSTRI TPKLLSLTW EGYPLHYTR EKGWGFLIP 
      FTDDTETSR KLPLAKLLE KCPLSKINT KEHDSSDTF 
      ANITQIVQD NLMRKEYYS KIRKDKTSG IWCSTEIED 
      CCYFLKLPH KNGAAYNVG NPLAKDFLN KFSENVLAG 
      DTQSAEQVL AIARTLWYW RNNRDRIFD QMVVWCDGA 
      QRGAALDDG AQRGAIIPQ VVVCGTLTR RAVEPTWMT 
      ASNARPDRV GSELRAMVQ APPGYNIIG ADVDSQELW 
      IASIIGDAD RARLHGATP FGWMTLSGT KADSTDMHS 
      VTAKAVGIS RDHAKVINY ARIYGAGQN FAERLLKQF 
      NPSMSDGEA KTKAAKMFA LTKGKRIYR LKKEFVLEY 
      YDQNYSHWQ AFEIAKTWN RRIDDMFER PKWVGGTES 
      AMFNRLESI ATSQEPRTP FLNGRLSRA LEPKLSSDD 
      RFLPTRINW VVQSGAADF LHLMLVCMR WIMGENARF 
      CLSFHDEVR YLVPERFKY QGALAMHVT NLLTRAFCS 
      VRLGIYDLP QSVAFFSSV EVDTVLRKD AKDDCMTPS 
      NPHGLEKGY NIPNGEGLD INQAVKKAG GKFSYWYEQ 
      LK
    CDS sequence
    • >TC008519 RA
      atgttctgt acgaaattg ctattttcg ccgaaacat 
      gaccaactc caacacgtt tttaaacac agttccttt 
      caccgcccc tctgtgcgt gtaaaaaaa ttcatccca 
      aaccctgac aaatcgaga ataaaaaaa acaattccg 
      ccctctcct tgcacattt gaggtctgt gtggggcct 
      cgcgtaaat caattcaac attcaaatg ttgtctccg 
      catcttttc gagcaagtt tttaaagga ctaagtgtg 
      aataagtgc gacgaagcg attgtgaaa gagtgtctc 
      aaaacgcta caagagcac aacatggta caaaaaccg 
      gagaatttt ttgaatgat gtggaattg aaaatacca 
      ccgcttgaa ggaaaaaat atcgaagaa cattttcat 
      ttaattggg gatcaacaa gttaaacca tatcgcgat 
      ttggtcgaa aaactttta acgaataaa atccctaaa 
      cctcctgat aagtgggta atgcaggag gggtggaca 
      agatatgtt ccaggagag gaaccacaa caagtgcca 
      tatccatta gaagaagga ttaattttt gatattgaa 
      gttgctgta acagtgggg aaagcaccg actttggct 
      acggctgtg agcaacgaa gcgtggtat ggatgggtt 
      agtcaaatg ttactggat ggtacaagc aaacctgta 
      acaacccat caatacaat ctcgatcaa ctcatacct 
      ctcgaaagt acaactaaa gacacaggc gcaaaactc 
      accaaccat atgaaaaaa ccaaaaatt ataataggc 
      cataacgtt tcatacgat cgtgccaga gtcaaagaa 
      caatattgg ttaaaccaa acggctaca agatttctc 
      gacacaatg agtctccat gtttgtgta agtggttta 
      accagttac caacgtgca atactcaaa tcgggcaaa 
      atcgatgaa gaagacgaa atttggaaa caacatagt 
      tccttgaac agtttaaac gaagttcac aagttgtac 
      tgtggcgga aaatcagta gcgaaagaa acgagagat 
      ctttttgtt acagggagt ttaaacgat attagggaa 
      gagtttcaa acagttatg aaatattgt agtggtgat 
      gttgtggca acttacagt gttttaaac gagttattt 
      ccgatgttt ttggaacgc tttccacat cctgtgacc 
      ttagcagga atgctcgaa ttaagtact ggttattta 
      ccgataaat tccaactgg aatcgatat attgataat 
      tctgaacaa gcctacgaa gatatggat attgagggg 
      aggctttta ttggcaaag agggcggag caggcgtgc 
      caactttta catgatgat aaatacaag gaagattta 
      tggctatgg gatgaagac tggacagta aaaaatctc 
      aaaataaag aaaacttct ccgaagact aaacaaatt 
      gaaacaccc ccaccacaa tcacccgat gatgaagca 
      actgaccct ctggaagaa aaattccgc gatttaatg 
      gcaacaaaa gccaacatt tcaagggtc aattcccac 
      ctccccggt tacccaaca tggtaccga aaactatct 
      acaaaacca gattctcca gattgggtt cctggtcct 
      cacttaatc agcacttcg acacgtatc acacctaaa 
      ctattatct ctaacgtgg gaaggctac cctttgcat 
      tacactcgt gaaaagggt tggggtttc ctcatcccc 
      ttcacagat gacactgaa acttcccga aaactacct 
      ttagcaaaa cttctcgaa aaatgtcca ttatcaaaa 
      atcaacaca aaggaacac gattcgagt gatactttc 
      gcaaacatc acacaaatc gtgcaagat aacttgatg 
      cgaaaagaa tactattcg aaaattcgc aaagataaa 
      acttcgggc atttggtgc agtaccgaa atcgaagat 
      tgttgctac ttcttgaaa ttaccgcac aaaaacggc 
      gctgcatac aacgtcggt aatccccta gctaaggat 
      tttttgaac aaattctcc gaaaacgta ctggctggt 
      gatacacaa agtgccgaa caagtcctg gcaatagcg 
      cgcactttg tggtactgg cgcaacaac cgcgaccgt 
      attttcgac caaatggtc gtttggtgt gatggcgcc 
      caacgtggg gccgcacta gatgatggc gcacaacgt 
      ggggcgatc atcccgcag gtcgtagtg tgcggtacg 
      ctgactcgg cgagccgtc gaaccgact tggatgacg 
      gcgtcgaac gcccgacct gatcgggtc ggttcggaa 
      ttgcgggca atggtgcaa gcaccacct ggttacaat 
      ataattggg gctgatgtc gattcgcaa gaattgtgg 
      attgcgtcg attattggt gatgccgac agggctagg 
      cttcatggg gctacgccc ttcggttgg atgacctta 
      agtggaact aaagccgac agtactgat atgcatagt 
      gtgactgct aaagcagtt ggtatatca cgtgaccat 
      gccaaagtt attaactat gcgcggatt tatggagct 
      gggcaaaat ttcgctgag cggctgttg aaacagttc 
      aatccgtcg atgtcggat ggtgaggcg aagactaaa 
      gctgcgaaa atgtttgcg ttgactaaa ggcaaacgg 
      atctatcgc ttgaaaaaa gagtttgtt ttggagtat 
      tacgaccaa aactactcc cattggcag gcgtttgaa 
      atagccaaa acctggaat agacgtatt gatgatatg 
      ttcgaaaga cccaaatgg gttggtgga accgaatca 
      gctatgttc aaccgtttg gaaagcatt gcaacaagt 
      caggaacca cgaactccg tttctaaac ggacgatta 
      agccgtgcc ttggaaccc aaactgtca agcgacgac 
      cggttctta ccgaccaga atcaattgg gttgtacaa 
      agtggcgcg gcagatttt ttgcacttg atgctagtg 
      tgtatgagg tggattatg ggcgaaaac gcgcgattc 
      tgtttgagt tttcacgat gaagtgagg tatttggtt 
      ccggaaaga tttaagtat caaggagca cttgcaatg 
      cacgtgacg aatctcttg acgcgagca ttttgttcg 
      gtgaggttg gggatttat gatttgccg cagtctgtg 
      gcgtttttt tcatctgtg gaggttgat actgtgttg 
      aggaaagat gcaaaggat gattgcatg acgccatcg 
      aatccgcat gggttggaa aagggttat aatattcca 
      aatggggaa gggttggat ataaaccaa gcggttaag 
      aaagcggga ggcaaattt agttattgg tatgaacag 
      ttgaaatga
    mRNA sequence
    • >TC008519 RA
      taagagtaa taattattg ttttgctga gcgttttta 
      cgaagcctc ttttgttat atttcaaat cagattcag 
      attaatcac tttggcggt tgttgtttt tagcagctg 
      gtgctttga ttgaggtta tgttcaatt tgttatagt 
      tcttgttcc aacaaatta cagaagaaa cattaattg 
      tgtgagtct tcctgagtg ttttttcga agccttgac 
      cgaaattat ttgcaggtg agtcggatt gttttaatt 
      caaaattgt aacctcaaa accttcgcg atgttctgt 
      acgaaattg ctattttcg ccgaaacat gaccaactc 
      caacacgtt tttaaacac agttccttt caccgcccc 
      tctgtgcgt gtaaaaaaa ttcatccca aaccctgac 
      aaatcgaga ataaaaaaa acaattccg ccctctcct 
      tgcacattt gaggtctgt gtggggcct cgcgtaaat 
      caattcaac attcaaatg ttgtctccg catcttttc 
      gagcaagtt tttaaagga ctaagtgtg aataagtgc 
      gacgaagcg attgtgaaa gagtgtctc aaaacgcta 
      caagagcac aacatggta caaaaaccg gagaatttt 
      ttgaatgat gtggaattg aaaatacca ccgcttgaa 
      ggaaaaaat atcgaagaa cattttcat ttaattggg 
      gatcaacaa gttaaacca tatcgcgat ttggtcgaa 
      aaactttta acgaataaa atccctaaa cctcctgat 
      aagtgggta atgcaggag gggtggaca agatatgtt 
      ccaggagag gaaccacaa caagtgcca tatccatta 
      gaagaagga ttaattttt gatattgaa gttgctgta 
      acagtgggg aaagcaccg actttggct acggctgtg 
      agcaacgaa gcgtggtat ggatgggtt agtcaaatg 
      ttactggat ggtacaagc aaacctgta acaacccat 
      caatacaat ctcgatcaa ctcatacct ctcgaaagt 
      acaactaaa gacacaggc gcaaaactc accaaccat 
      atgaaaaaa ccaaaaatt ataataggc cataacgtt 
      tcatacgat cgtgccaga gtcaaagaa caatattgg 
      ttaaaccaa acggctaca agatttctc gacacaatg 
      agtctccat gtttgtgta agtggttta accagttac 
      caacgtgca atactcaaa tcgggcaaa atcgatgaa 
      gaagacgaa atttggaaa caacatagt tccttgaac 
      agtttaaac gaagttcac aagttgtac tgtggcgga 
      aaatcagta gcgaaagaa acgagagat ctttttgtt 
      acagggagt ttaaacgat attagggaa gagtttcaa 
      acagttatg aaatattgt agtggtgat gttgtggca 
      acttacagt gttttaaac gagttattt ccgatgttt 
      ttggaacgc tttccacat cctgtgacc ttagcagga 
      atgctcgaa ttaagtact ggttattta ccgataaat 
      tccaactgg aatcgatat attgataat tctgaacaa 
      gcctacgaa gatatggat attgagggg aggctttta 
      ttggcaaag agggcggag caggcgtgc caactttta 
      catgatgat aaatacaag gaagattta tggctatgg 
      gatgaagac tggacagta aaaaatctc aaaataaag 
      aaaacttct ccgaagact aaacaaatt gaaacaccc 
      ccaccacaa tcacccgat gatgaagca actgaccct 
      ctggaagaa aaattccgc gatttaatg gcaacaaaa 
      gccaacatt tcaagggtc aattcccac ctccccggt 
      tacccaaca tggtaccga aaactatct acaaaacca 
      gattctcca gattgggtt cctggtcct cacttaatc 
      agcacttcg acacgtatc acacctaaa ctattatct 
      ctaacgtgg gaaggctac cctttgcat tacactcgt 
      gaaaagggt tggggtttc ctcatcccc ttcacagat 
      gacactgaa acttcccga aaactacct ttagcaaaa 
      cttctcgaa aaatgtcca ttatcaaaa atcaacaca 
      aaggaacac gattcgagt gatactttc gcaaacatc 
      acacaaatc gtgcaagat aacttgatg cgaaaagaa 
      tactattcg aaaattcgc aaagataaa acttcgggc 
      atttggtgc agtaccgaa atcgaagat tgttgctac 
      ttcttgaaa ttaccgcac aaaaacggc gctgcatac 
      aacgtcggt aatccccta gctaaggat tttttgaac 
      aaattctcc gaaaacgta ctggctggt gatacacaa 
      agtgccgaa caagtcctg gcaatagcg cgcactttg 
      tggtactgg cgcaacaac cgcgaccgt attttcgac 
      caaatggtc gtttggtgt gatggcgcc caacgtggg 
      gccgcacta gatgatggc gcacaacgt ggggcgatc 
      atcccgcag gtcgtagtg tgcggtacg ctgactcgg 
      cgagccgtc gaaccgact tggatgacg gcgtcgaac 
      gcccgacct gatcgggtc ggttcggaa ttgcgggca 
      atggtgcaa gcaccacct ggttacaat ataattggg 
      gctgatgtc gattcgcaa gaattgtgg attgcgtcg 
      attattggt gatgccgac agggctagg cttcatggg 
      gctacgccc ttcggttgg atgacctta agtggaact 
      aaagccgac agtactgat atgcatagt gtgactgct 
      aaagcagtt ggtatatca cgtgaccat gccaaagtt 
      attaactat gcgcggatt tatggagct gggcaaaat 
      ttcgctgag cggctgttg aaacagttc aatccgtcg 
      atgtcggat ggtgaggcg aagactaaa gctgcgaaa 
      atgtttgcg ttgactaaa ggcaaacgg atctatcgc 
      ttgaaaaaa gagtttgtt ttggagtat tacgaccaa 
      aactactcc cattggcag gcgtttgaa atagccaaa 
      acctggaat agacgtatt gatgatatg ttcgaaaga 
      cccaaatgg gttggtgga accgaatca gctatgttc 
      aaccgtttg gaaagcatt gcaacaagt caggaacca 
      cgaactccg tttctaaac ggacgatta agccgtgcc 
      ttggaaccc aaactgtca agcgacgac cggttctta 
      ccgaccaga atcaattgg gttgtacaa agtggcgcg 
      gcagatttt ttgcacttg atgctagtg tgtatgagg 
      tggattatg ggcgaaaac gcgcgattc tgtttgagt 
      tttcacgat gaagtgagg tatttggtt ccggaaaga 
      tttaagtat caaggagca cttgcaatg cacgtgacg 
      aatctcttg acgcgagca ttttgttcg gtgaggttg 
      gggatttat gatttgccg cagtctgtg gcgtttttt 
      tcatctgtg gaggttgat actgtgttg aggaaagat 
      gcaaaggat gattgcatg acgccatcg aatccgcat 
      gggttggaa aagggttat aatattcca aatggggaa 
      gggttggat ataaaccaa gcggttaag aaagcggga 
      ggcaaattt agttattgg tatgaacag ttgaaatga 
      ttgtgttat gttgattaa taaaaaaat tataagtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY