| General information | |||
| TC008283 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 7866754..7870358 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008283 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06254 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC008283 RA
MDKNRDNLL HRIIIEKVN NTIDAPLAK LINESESHD VAEIIKKLD LQSIEDDIL KSAKRHHNV ESWRVDLTQ NDYSAIIEG LNSNSTSTK LSVLDKLLN IDIDTKQIF WEELSQSLR KCFVSATYD IFLRTLKIH HRLSNTSSD GYLNLLRGT FLLLNSRYF TDFNKVRIL QSLKVVLDT QKAVMKFLL HANHTTIDE ITTAFISVL TYKTVFDWF GALDPQSNW IRVFCYGAD IRNIFFNNL KEKKLNFIK NVMATFLQK INSSEEDWG VHYGHFLLY FWRYNNNNT TFVTDTDEG FKAIINKLS QGKRTELTD LFIDLFTHN PSILTPQLL DALVAPLNN TNRNNIRNL VETNKFVFA IINNVTKSG NHRVLFGVS GTLRKARAR QVVRNVTNV PQKITDLAV LLLKTNVNT NELNQDLGS LEILLHCCL CLYENHPLA LLHGNPAKL LQSMNEFYQ TNRKTHNTF LPLFNFFCA NFGRTIRIF DWNSEIYTD LFANVPVEN LKSVITRLG SDKLGFEFL NKRHRQIVQ PYLEEILIS DETVNSNSS TKFVLFLFV VKLNARSAV ALLADDDGD TVVESEDKS VTLSELIEK SLSESVEPN EEYLGLLTM KVFITNLDL LLYLQTNYQ MQKKLEEAA RCSDIIDAQ SCLRDNILV SLSHVGFVN KSGDTPPVY EILEQGKSG ENEFRVFLD EDTTGNFSW LSQARKAFK NSLHLFDPS ILEILLEKI PKTYVYRKI ELQWPPSDS VYYQLHPED FSGVSLIID YGLKTGLLK PPNEYSENL IFLIKQSRA FIGYQPENF TNFDWFVGF IFLTCSGDL HKCKSILIN FTNTQVTSL IWATFGKEF DYLLQESFH QHFAVVLEE NCSGYQALK VLGICPEIL LQSWLNQCF FNFLRFEEI CNFLAFSVL YPIECLMYY FVAIFRHLD DRICTITHS PELQEILEM CDMSEFRLS DHMPLIEKL TKCYRNTHK L
- >TC008283 RA
atggataaa aatcgtgat aatttattg caccgaata attatagag aaagttaat aatacaatc gacgctcca ctcgcgaag ttaattaac gaaagtgaa tcacacgac gttgcagaa attattaaa aaactagac ttgcaatca atcgaagac gatattctg aaaagtgcg aaaagacac cacaatgtc gagtcgtgg agagttgac ctcactcaa aacgactat tctgcgata attgaaggc ctcaacagc aattcgacc tcaactaaa ctttcagtg ctcgataaa ctcctcaat atcgacata gacaccaaa caaattttc tgggaagag ttgtcgcaa agtctgcgg aaatgtttc gtaagcgcc acttacgac atttttttg cgcactctt aaaatccac caccggctc tcaaacacc tcaagcgac ggttatttg aacctactt agaggcact ttcttgctt ttaaacagt cgctacttt accgatttc aataaagta cgcattttg caaagtctc aaagttgtt ttggacaca caaaaggcc gtaatgaaa tttctactg cacgctaat cacactact attgacgaa attaccact gcttttatc agcgttctg acgtacaaa acggttttt gattggttt ggggcacta gacccacag tcaaattgg atccgggtt ttttgctac ggtgccgac attcgcaat atttttttc aacaactta aaagagaaa aaactgaat tttatcaaa aatgttatg gcgactttt ttgcaaaaa attaacagt agcgaggaa gattgggga gttcattat ggccacttt ctactctat ttttggcga tataataat aataataca actttcgtt accgacaca gacgagggt tttaaggca ataattaac aagttaagt cagggaaaa agaacggaa ctgacggat ctctttatt gatttattc acacacaac ccttcaatt ctcaccccc caattgtta gatgccttg gttgcgccc ttaaataac acgaaccga aacaatatc cgaaatttg gtcgaaaca aataaattt gtttttgcc ataattaat aacgtcaca aaatcgggg aatcaccgg gttttgttc ggcgtttct ggtacttta cgtaaagct agagcgcga caagtggtc cgaaacgtc acaaatgtt cctcaaaaa atcaccgat ttggctgta ttgctcctc aaaacgaac gtaaacacg aatgaatta aatcaggac ttgggcagt cttgaaatt ttattgcac tgttgtctc tgcctttac gagaaccac cccttagcc ctcctccac ggaaatccc gccaaattg cttcagagt atgaacgaa ttttatcaa acaaatcgc aaaactcac aatactttt cttcctctg tttaatttt ttttgtgca aactttggg cgcacaatt aggattttt gattggaat tctgaaatt tacacggat ttgtttgcg aacgtcccg gttgagaat ttaaaaagt gttataact cgactgggg agtgacaaa ctgggtttt gagtttttg aacaagcgc cacagacaa atcgttcaa ccttacctt gaagaaatt ttgatttcg gacgaaact gttaactca aactcgagt actaaattc gtgttattt cttttcgtt gttaaatta aacgcacga agtgctgtc gcgttgtta gctgacgat gacggcgac actgtggtc gaaagtgag gataaaagt gttaccctg tccgaacta atagagaaa agcctaagc gagtccgtt gagccaaat gaagagtat ttgggattg ttaacaatg aaagtattc attacaaat ttagatctg ttgctctac ttgcaaact aattatcaa atgcaaaaa aaattggag gaagctgcc agatgcagc gacattatc gatgcacaa agctgttta agagataat attttagtt tcgttgtca cacgtaggg tttgtcaat aagtctgga gatacacct cccgtttac gaaattcta gagcaagga aaaagtggg gaaaatgaa tttcgggtg tttttggat gaggacaca actggaaat ttcagctgg ttgtcgcag gcgagaaaa gccttcaaa aactcattg catcttttt gatccttct atcttggaa attttattg gaaaaaatt cccaaaact tacgtttac agaaaaatc gagctacaa tggccccct tcagattcc gtctactac cagttgcac cccgaagac ttttctggc gtttcctta ataatcgat tatggactc aaaactggc ttacttaaa ccacctaat gaatactcc gaaaatctt atttttctg ataaaacaa tcacgggct tttattggt taccaaccg gaaaatttc accaatttc gattggttt gtcggattc atttttctg acgtgttcg ggagattta cataagtgc aaatccatt ttgattaat tttacaaac acgcaagtg acgtcgctt atttgggcc acttttggc aaagagttt gattatttg ttacaagag agcttccac caacatttt gcagttgtt ttggaagag aactgctct ggctaccaa gccttgaag gttttaggt atttgtcct gaaatcctg ctccagtca tggctcaac cagtgcttc tttaatttc cttcgtttt gaagaaatc tgtaatttc ttagcattc tcagttttg tatcctatt gagtgtctt atgtattat tttgttgct attttccga catttggac gaccggatt tgtacaatc acacattca cctgagctc caagaaata cttgagatg tgcgacatg agcgagttt cgtttaagc gaccacatg cccctaatc gagaaactt acgaaatgt taccgcaac acgcataaa ttataa
- >TC008283 RA
tttgacatg acaacaaat agacgtcaa aaatggata aaaatcgtg ataatttat tgcaccgaa taattatag agaaagtta ataatacaa tcgacgctc cactcgcga agttaatta acgaaagtg aatcacacg acgttgcag aaattatta aaaaactag acttgcaat caatcgaag acgatattc tgaaaagtg cgaaaagac accacaatg tcgagtcgt ggagagttg acctcactc aaaacgact attctgcga taattgaag gcctcaaca gcaattcga cctcaacta aactttcag tgctcgata aactcctca atatcgaca tagacacca aacaaattt tctgggaag agttgtcgc aaagtctgc ggaaatgtt tcgtaagcg ccacttacg acatttttt tgcgcactc ttaaaatcc accaccggc tctcaaaca cctcaagcg acggttatt tgaacctac ttagaggca ctttcttgc ttttaaaca gtcgctact ttaccgatt tcaataaag tacgcattt tgcaaagtc tcaaagttg ttttggaca cacaaaagg ccgtaatga aatttctac tgcacgcta atcacacta ctattgacg aaattacca ctgctttta tcagcgttc tgacgtaca aaacggttt ttgattggt ttggggcac tagacccac agtcaaatt ggatccggg ttttttgct acggtgccg acattcgca atatttttt tcaacaact taaaagaga aaaaactga attttatca aaaatgtta tggcgactt ttttgcaaa aaattaaca gtagcgagg aagattggg gagttcatt atggccact ttctactct atttttggc gatataata ataataata caactttcg ttaccgaca cagacgagg gttttaagg caataatta acaagttaa gtcagggaa aaagaacgg aactgacgg atctcttta ttgatttat tcacacaca acccttcaa ttctcaccc cccaattgt tagatgcct tggttgcgc ccttaaata acacgaacc gaaacaata tccgaaatt tggtcgaaa caaataaat ttgtttttg ccataatta ataacgtca caaaatcgg ggaatcacc gggttttgt tcggcgttt ctggtactt tacgtaaag ctagagcgc gacaagtgg tccgaaacg tcacaaatg ttcctcaaa aaatcaccg atttggctg tattgctcc tcaaaacga acgtaaaca cgaatgaat taaatcagg acttgggca gtcttgaaa ttttattgc actgttgtc tctgccttt acgagaacc accccttag ccctcctcc acggaaatc ccgccaaat tgcttcaga gtatgaacg aattttatc aaacaaatc gcaaaactc acaatactt ttcttcctc tgtttaatt ttttttgtg caaactttg ggcgcacaa ttaggattt ttgattgga attctgaaa tttacacgg atttgtttg cgaacgtcc cggttgaga atttaaaaa gtgttataa ctcgactgg ggagtgaca aactgggtt ttgagtttt tgaacaagc gccacagac aaatcgttc aaccttacc ttgaagaaa ttttgattt cggacgaaa ctgttaact caaactcga gtactaaat tcgtgttat ttcttttcg ttgttaaat taaacgcac gaagtgctg tcgcgttgt tagctgacg atgacggcg acactgtgg tcgaaagtg aggataaaa gtgttaccc tgtccgaac taatagaga aaagcctaa gcgagtccg ttgagccaa atgaagagt atttgggat tgttaacaa tgaaagtat tcattacaa atttagatc tgttgctct acttgcaaa ctaattatc aaatgcaaa aaaaattgg aggaagctg ccagatgca gcgacatta tcgatgcac aaagctgtt taagagata atattttag tttcgttgt cacacgtag ggtttgtca ataagtctg gagatacac ctcccgttt acgaaattc tagagcaag gaaaaagtg gggaaaatg aatttcggg tgtttttgg atgaggaca caactggaa atttcagct ggttgtcgc aggcgagaa aagccttca aaaactcat tgcatcttt ttgatcctt ctatcttgg aaattttat tggaaaaaa ttcccaaaa cttacgttt acagaaaaa tcgagctac aatggcccc cttcagatt ccgtctact accagttgc accccgaag acttttctg gcgtttcct taataatcg attatggac tcaaaactg gcttactta aaccaccta atgaatact ccgaaaatc ttatttttc tgataaaac aatcacggg cttttattg gttaccaac cggaaaatt tcaccaatt tcgattggt ttgtcggat tcatttttc tgacgtgtt cgggagatt tacataagt gcaaatcca ttttgatta attttacaa acacgcaag tgacgtcgc ttatttggg ccacttttg gcaaagagt ttgattatt tgttacaag agagcttcc accaacatt ttgcagttg ttttggaag agaactgct ctggctacc aagccttga aggttttag gtatttgtc ctgaaatcc tgctccagt catggctca accagtgct tctttaatt tccttcgtt ttgaagaaa tctgtaatt tcttagcat tctcagttt tgtatccta ttgagtgtc ttatgtatt attttgttg ctattttcc gacatttgg acgaccgga tttgtacaa tcacacatt cacctgagc tccaagaaa tacttgaga tgtgcgaca tgagcgagt ttcgtttaa gcgaccaca tgcccctaa tcgagaaac ttacgaaat gttaccgca acacgcata aattataat aaagcagaa gttttgaca aa
