Status
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Gene information
General information
TC008283 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7866754..7870358
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008283
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06254

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008283 RA
        MDKNRDNLL HRIIIEKVN NTIDAPLAK LINESESHD 
        VAEIIKKLD LQSIEDDIL KSAKRHHNV ESWRVDLTQ 
        NDYSAIIEG LNSNSTSTK LSVLDKLLN IDIDTKQIF 
        WEELSQSLR KCFVSATYD IFLRTLKIH HRLSNTSSD 
        GYLNLLRGT FLLLNSRYF TDFNKVRIL QSLKVVLDT 
        QKAVMKFLL HANHTTIDE ITTAFISVL TYKTVFDWF 
        GALDPQSNW IRVFCYGAD IRNIFFNNL KEKKLNFIK 
        NVMATFLQK INSSEEDWG VHYGHFLLY FWRYNNNNT 
        TFVTDTDEG FKAIINKLS QGKRTELTD LFIDLFTHN 
        PSILTPQLL DALVAPLNN TNRNNIRNL VETNKFVFA 
        IINNVTKSG NHRVLFGVS GTLRKARAR QVVRNVTNV 
        PQKITDLAV LLLKTNVNT NELNQDLGS LEILLHCCL 
        CLYENHPLA LLHGNPAKL LQSMNEFYQ TNRKTHNTF 
        LPLFNFFCA NFGRTIRIF DWNSEIYTD LFANVPVEN 
        LKSVITRLG SDKLGFEFL NKRHRQIVQ PYLEEILIS 
        DETVNSNSS TKFVLFLFV VKLNARSAV ALLADDDGD 
        TVVESEDKS VTLSELIEK SLSESVEPN EEYLGLLTM 
        KVFITNLDL LLYLQTNYQ MQKKLEEAA RCSDIIDAQ 
        SCLRDNILV SLSHVGFVN KSGDTPPVY EILEQGKSG 
        ENEFRVFLD EDTTGNFSW LSQARKAFK NSLHLFDPS 
        ILEILLEKI PKTYVYRKI ELQWPPSDS VYYQLHPED 
        FSGVSLIID YGLKTGLLK PPNEYSENL IFLIKQSRA 
        FIGYQPENF TNFDWFVGF IFLTCSGDL HKCKSILIN 
        FTNTQVTSL IWATFGKEF DYLLQESFH QHFAVVLEE 
        NCSGYQALK VLGICPEIL LQSWLNQCF FNFLRFEEI 
        CNFLAFSVL YPIECLMYY FVAIFRHLD DRICTITHS 
        PELQEILEM CDMSEFRLS DHMPLIEKL TKCYRNTHK 
        L
      CDS sequence
      • >TC008283 RA
        atggataaa aatcgtgat aatttattg caccgaata 
        attatagag aaagttaat aatacaatc gacgctcca 
        ctcgcgaag ttaattaac gaaagtgaa tcacacgac 
        gttgcagaa attattaaa aaactagac ttgcaatca 
        atcgaagac gatattctg aaaagtgcg aaaagacac 
        cacaatgtc gagtcgtgg agagttgac ctcactcaa 
        aacgactat tctgcgata attgaaggc ctcaacagc 
        aattcgacc tcaactaaa ctttcagtg ctcgataaa 
        ctcctcaat atcgacata gacaccaaa caaattttc 
        tgggaagag ttgtcgcaa agtctgcgg aaatgtttc 
        gtaagcgcc acttacgac atttttttg cgcactctt 
        aaaatccac caccggctc tcaaacacc tcaagcgac 
        ggttatttg aacctactt agaggcact ttcttgctt 
        ttaaacagt cgctacttt accgatttc aataaagta 
        cgcattttg caaagtctc aaagttgtt ttggacaca 
        caaaaggcc gtaatgaaa tttctactg cacgctaat 
        cacactact attgacgaa attaccact gcttttatc 
        agcgttctg acgtacaaa acggttttt gattggttt 
        ggggcacta gacccacag tcaaattgg atccgggtt 
        ttttgctac ggtgccgac attcgcaat atttttttc 
        aacaactta aaagagaaa aaactgaat tttatcaaa 
        aatgttatg gcgactttt ttgcaaaaa attaacagt 
        agcgaggaa gattgggga gttcattat ggccacttt 
        ctactctat ttttggcga tataataat aataataca 
        actttcgtt accgacaca gacgagggt tttaaggca 
        ataattaac aagttaagt cagggaaaa agaacggaa 
        ctgacggat ctctttatt gatttattc acacacaac 
        ccttcaatt ctcaccccc caattgtta gatgccttg 
        gttgcgccc ttaaataac acgaaccga aacaatatc 
        cgaaatttg gtcgaaaca aataaattt gtttttgcc 
        ataattaat aacgtcaca aaatcgggg aatcaccgg 
        gttttgttc ggcgtttct ggtacttta cgtaaagct 
        agagcgcga caagtggtc cgaaacgtc acaaatgtt 
        cctcaaaaa atcaccgat ttggctgta ttgctcctc 
        aaaacgaac gtaaacacg aatgaatta aatcaggac 
        ttgggcagt cttgaaatt ttattgcac tgttgtctc 
        tgcctttac gagaaccac cccttagcc ctcctccac 
        ggaaatccc gccaaattg cttcagagt atgaacgaa 
        ttttatcaa acaaatcgc aaaactcac aatactttt 
        cttcctctg tttaatttt ttttgtgca aactttggg 
        cgcacaatt aggattttt gattggaat tctgaaatt 
        tacacggat ttgtttgcg aacgtcccg gttgagaat 
        ttaaaaagt gttataact cgactgggg agtgacaaa 
        ctgggtttt gagtttttg aacaagcgc cacagacaa 
        atcgttcaa ccttacctt gaagaaatt ttgatttcg 
        gacgaaact gttaactca aactcgagt actaaattc 
        gtgttattt cttttcgtt gttaaatta aacgcacga 
        agtgctgtc gcgttgtta gctgacgat gacggcgac 
        actgtggtc gaaagtgag gataaaagt gttaccctg 
        tccgaacta atagagaaa agcctaagc gagtccgtt 
        gagccaaat gaagagtat ttgggattg ttaacaatg 
        aaagtattc attacaaat ttagatctg ttgctctac 
        ttgcaaact aattatcaa atgcaaaaa aaattggag 
        gaagctgcc agatgcagc gacattatc gatgcacaa 
        agctgttta agagataat attttagtt tcgttgtca 
        cacgtaggg tttgtcaat aagtctgga gatacacct 
        cccgtttac gaaattcta gagcaagga aaaagtggg 
        gaaaatgaa tttcgggtg tttttggat gaggacaca 
        actggaaat ttcagctgg ttgtcgcag gcgagaaaa 
        gccttcaaa aactcattg catcttttt gatccttct 
        atcttggaa attttattg gaaaaaatt cccaaaact 
        tacgtttac agaaaaatc gagctacaa tggccccct 
        tcagattcc gtctactac cagttgcac cccgaagac 
        ttttctggc gtttcctta ataatcgat tatggactc 
        aaaactggc ttacttaaa ccacctaat gaatactcc 
        gaaaatctt atttttctg ataaaacaa tcacgggct 
        tttattggt taccaaccg gaaaatttc accaatttc 
        gattggttt gtcggattc atttttctg acgtgttcg 
        ggagattta cataagtgc aaatccatt ttgattaat 
        tttacaaac acgcaagtg acgtcgctt atttgggcc 
        acttttggc aaagagttt gattatttg ttacaagag 
        agcttccac caacatttt gcagttgtt ttggaagag 
        aactgctct ggctaccaa gccttgaag gttttaggt 
        atttgtcct gaaatcctg ctccagtca tggctcaac 
        cagtgcttc tttaatttc cttcgtttt gaagaaatc 
        tgtaatttc ttagcattc tcagttttg tatcctatt 
        gagtgtctt atgtattat tttgttgct attttccga 
        catttggac gaccggatt tgtacaatc acacattca 
        cctgagctc caagaaata cttgagatg tgcgacatg 
        agcgagttt cgtttaagc gaccacatg cccctaatc 
        gagaaactt acgaaatgt taccgcaac acgcataaa 
        ttataa
      mRNA sequence
      • >TC008283 RA
        tttgacatg acaacaaat agacgtcaa aaatggata 
        aaaatcgtg ataatttat tgcaccgaa taattatag 
        agaaagtta ataatacaa tcgacgctc cactcgcga 
        agttaatta acgaaagtg aatcacacg acgttgcag 
        aaattatta aaaaactag acttgcaat caatcgaag 
        acgatattc tgaaaagtg cgaaaagac accacaatg 
        tcgagtcgt ggagagttg acctcactc aaaacgact 
        attctgcga taattgaag gcctcaaca gcaattcga 
        cctcaacta aactttcag tgctcgata aactcctca 
        atatcgaca tagacacca aacaaattt tctgggaag 
        agttgtcgc aaagtctgc ggaaatgtt tcgtaagcg 
        ccacttacg acatttttt tgcgcactc ttaaaatcc 
        accaccggc tctcaaaca cctcaagcg acggttatt 
        tgaacctac ttagaggca ctttcttgc ttttaaaca 
        gtcgctact ttaccgatt tcaataaag tacgcattt 
        tgcaaagtc tcaaagttg ttttggaca cacaaaagg 
        ccgtaatga aatttctac tgcacgcta atcacacta 
        ctattgacg aaattacca ctgctttta tcagcgttc 
        tgacgtaca aaacggttt ttgattggt ttggggcac 
        tagacccac agtcaaatt ggatccggg ttttttgct 
        acggtgccg acattcgca atatttttt tcaacaact 
        taaaagaga aaaaactga attttatca aaaatgtta 
        tggcgactt ttttgcaaa aaattaaca gtagcgagg 
        aagattggg gagttcatt atggccact ttctactct 
        atttttggc gatataata ataataata caactttcg 
        ttaccgaca cagacgagg gttttaagg caataatta 
        acaagttaa gtcagggaa aaagaacgg aactgacgg 
        atctcttta ttgatttat tcacacaca acccttcaa 
        ttctcaccc cccaattgt tagatgcct tggttgcgc 
        ccttaaata acacgaacc gaaacaata tccgaaatt 
        tggtcgaaa caaataaat ttgtttttg ccataatta 
        ataacgtca caaaatcgg ggaatcacc gggttttgt 
        tcggcgttt ctggtactt tacgtaaag ctagagcgc 
        gacaagtgg tccgaaacg tcacaaatg ttcctcaaa 
        aaatcaccg atttggctg tattgctcc tcaaaacga 
        acgtaaaca cgaatgaat taaatcagg acttgggca 
        gtcttgaaa ttttattgc actgttgtc tctgccttt 
        acgagaacc accccttag ccctcctcc acggaaatc 
        ccgccaaat tgcttcaga gtatgaacg aattttatc 
        aaacaaatc gcaaaactc acaatactt ttcttcctc 
        tgtttaatt ttttttgtg caaactttg ggcgcacaa 
        ttaggattt ttgattgga attctgaaa tttacacgg 
        atttgtttg cgaacgtcc cggttgaga atttaaaaa 
        gtgttataa ctcgactgg ggagtgaca aactgggtt 
        ttgagtttt tgaacaagc gccacagac aaatcgttc 
        aaccttacc ttgaagaaa ttttgattt cggacgaaa 
        ctgttaact caaactcga gtactaaat tcgtgttat 
        ttcttttcg ttgttaaat taaacgcac gaagtgctg 
        tcgcgttgt tagctgacg atgacggcg acactgtgg 
        tcgaaagtg aggataaaa gtgttaccc tgtccgaac 
        taatagaga aaagcctaa gcgagtccg ttgagccaa 
        atgaagagt atttgggat tgttaacaa tgaaagtat 
        tcattacaa atttagatc tgttgctct acttgcaaa 
        ctaattatc aaatgcaaa aaaaattgg aggaagctg 
        ccagatgca gcgacatta tcgatgcac aaagctgtt 
        taagagata atattttag tttcgttgt cacacgtag 
        ggtttgtca ataagtctg gagatacac ctcccgttt 
        acgaaattc tagagcaag gaaaaagtg gggaaaatg 
        aatttcggg tgtttttgg atgaggaca caactggaa 
        atttcagct ggttgtcgc aggcgagaa aagccttca 
        aaaactcat tgcatcttt ttgatcctt ctatcttgg 
        aaattttat tggaaaaaa ttcccaaaa cttacgttt 
        acagaaaaa tcgagctac aatggcccc cttcagatt 
        ccgtctact accagttgc accccgaag acttttctg 
        gcgtttcct taataatcg attatggac tcaaaactg 
        gcttactta aaccaccta atgaatact ccgaaaatc 
        ttatttttc tgataaaac aatcacggg cttttattg 
        gttaccaac cggaaaatt tcaccaatt tcgattggt 
        ttgtcggat tcatttttc tgacgtgtt cgggagatt 
        tacataagt gcaaatcca ttttgatta attttacaa 
        acacgcaag tgacgtcgc ttatttggg ccacttttg 
        gcaaagagt ttgattatt tgttacaag agagcttcc 
        accaacatt ttgcagttg ttttggaag agaactgct 
        ctggctacc aagccttga aggttttag gtatttgtc 
        ctgaaatcc tgctccagt catggctca accagtgct 
        tctttaatt tccttcgtt ttgaagaaa tctgtaatt 
        tcttagcat tctcagttt tgtatccta ttgagtgtc 
        ttatgtatt attttgttg ctattttcc gacatttgg 
        acgaccgga tttgtacaa tcacacatt cacctgagc 
        tccaagaaa tacttgaga tgtgcgaca tgagcgagt 
        ttcgtttaa gcgaccaca tgcccctaa tcgagaaac 
        ttacgaaat gttaccgca acacgcata aattataat 
        aaagcagaa gttttgaca aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY