Status
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Gene information
General information
TC008156 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5859118..5871357
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008156
    iBeetle screen iB_06252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008156 RA
      MPVDERVAR SVALEQAYV HDVYEQFYD NPRSKPWPK 
      VQQFLQDLE PGSLVCDVG CGNGKYLNV NTSIFNMGG 
      DKSMRLTEV ARDKENEVI ALDNLALPF RDESLDAVL 
      SIAVVHHLA TTERRICAL RELARVLRI GGRLIISVW 
      AMEQSHRKF ESQDVLVPW HRPKHLSTP SLELTSTTN 
      TSEDDCLPP YHAYTQTSD SDSNRSVKS KGIVKKRGK 
      SRHKGRSID PGRNSSPSS SSLSSPNET CYSFVRRAI 
      QKLAGGRRV GVHRPWFLE SWSTCAKEP SHQQRYDHD 
      GCECCDCNE NIQDIPIEL RRVDEEDLP QRRQTFPSS 
      QLVNDISSN LKSKSMTNI KTVENNNIV RSQSSTPSV 
      PEINVLPEI KPKATLAKP KLVKQKKSI CEEDDEEAL 
      DQPTDMKTL INNLPDFKV FHARYHRGG VFKQRSLNE 
      ELMSTDRLK EKERVRQNI QKQASLNED LIYQRHTFD 
      SFKDSFFSV STSKRFQLI KTGFTNKIK NSTTIEKVT 
      GTSLKNGFV RMFQNWKSS DLISPTIPE NETIDPLKK 
      PIVIEEKKD GSTTERRHS KEDGSDSSK DSSLQSDTS 
      VDSEDSFAS VIFVPKSDS MSPTPLSPG PISPRVKTS 
      SVPNSPRIK QSSCPTSPR IKQMPLTVY PLTKQLSSP 
      KPTTSDLLT DKSDRDHTA STTASSKNL AQKYSVPQI 
      PKFRRTSLN SPSLNTSPE KSPVEKTET RTERLKKIK 
      ELLTQKPGF GARSKSNYP IVRHSSVAN GKVEAVAKP 
      LPKLLTLEL FNPELDDKD SDSSCVSSP DSIDSVINV 
      RTDITRTKF KFPPSKPKD PKPMSLLEA AADVANTLD 
      EAVEKVIKS SPRTKRKLQ TGDVLSIIQ RRYSSDTTP 
      LLGGPSWDE ECHKHLTDF ADKLSEKLL QEIDQYQEK 
      SKALTTSID NIDDPYIHR LSEELDDLS KLSAEIQKQ 
      NEYLAKLSA SDSLFNRAC AKCREVECK CYERTKTKE 
      KTDTKKVPV SEFFSVKND AFKRSHTNE SLSETPEFA 
      NCKLSVSKT SIRMGTSTD SCDSDKGSS TNSMISSVA 
      TSINFGQSL ESADGVESH KDTNSISRY SDGGSTASL 
      ASCPEWKTI KPKTEERLS NSTVSLESD SSKKREKST 
      LLSDTSQDS LPSDNIGGE ITYHRYYHV FREGELDQL 
      IEKYVENLH IISSYYDHA SWCIIAEKV QVWTI
    CDS sequence
    • >TC008156 RA
      atgccggtg gatgagcgt gtggcacga agcgttgct 
      ctagagcaa gcctacgtg cacgacgtc tacgaacaa 
      ttctacgac aaccccaga agcaagccc tggcccaaa 
      gtacaacag tttctgcag gatctcgaa cccgggtcc 
      ctggtctgc gacgtcggt tgtggaaat gggaagtat 
      ttgaacgtg aacacttcg atattcaac atgggaggc 
      gataaatcg atgcgattg actgaagtg gctcgagac 
      aaggagaac gaagttata gccctcgac aacttagcg 
      ctgcctttc cgcgacgaa agcctcgat gccgtgctt 
      tccattgcc gtcgttcac catctggcg acaacggaa 
      agaagaatc tgcgccctg agggagctg gccagagtt 
      ctacggatc ggcggccga ctcatcatc tcagtttgg 
      gccatggaa cagagccat cgcaagttc gagtctcag 
      gacgtccta gttccctgg cacaggcct aagcatcta 
      agcacgccc tccctcgaa ctcacctcc acgactaac 
      acctcggaa gacgactgc ttaccgcca tatcacgcc 
      tacacgcaa acttccgac agcgactcc aacagatcc 
      gtaaagtca aagggcatt gtgaaaaag cgggggaag 
      tcccggcac aagggccgt tcgatcgac cccggacgt 
      aactcttct cccagcagt tccagttta tcaagtccc 
      aacgaaact tgctacagt ttcgtgcgc cgggctatc 
      cagaaattg gcagggggg cgaagagtg ggtgtgcac 
      aggccctgg tttttggaa agttggagc acttgtgct 
      aaagagccg tcgcatcaa cagcgttac gaccatgac 
      ggatgcgaa tgctgcgac tgtaatgag aacattcaa 
      gatattcct atcgagttg aggagggtt gacgaagag 
      gatttgcct cagaggcgg caaactttt ccgtcgtct 
      cagcttgtt aacgatatt tccagcaac ttgaaatcg 
      aagagtatg acgaatatt aagactgtt gaaaataat 
      aatatagtg aggtcgcaa tcgagcacg ccgagtgta 
      ccggaaatc aacgtgttg ccggaaatt aagccgaag 
      gctacgctc gccaagccc aagctggtt aagcagaaa 
      aaatcgatc tgtgaggag gatgatgag gaggctttg 
      gatcaaccg actgatatg aaaacgctg attaataat 
      ctaccggac tttaaggtg tttcatgcg aggtatcac 
      agagggggg gtgtttaag cagcggtcg ctgaacgag 
      gagttgatg tcgacggat agactgaag gagaaggag 
      agggtgcgg caaaatatc cagaaacaa gcatctttg 
      aacgaggat ttaatttat caaagacac actttcgac 
      tcattcaaa gattccttc ttttctgtt tctacttcg 
      aagcgcttc caattaatc aaaacgggt tttacgaat 
      aaaattaaa aactccact acaatagag aaagtgact 
      gggacatcg ctgaaaaac gggttcgtc aggatgttc 
      caaaactgg aaaagttcc gacctgatt tcgcccacg 
      atccccgaa aacgaaaca atcgaccct ttgaaaaag 
      ccaatagtg atcgaagag aaaaaggac ggttctacg 
      accgaacgg agacactcc aaagaggac ggttccgac 
      tcatccaaa gacagtagt ttgcaaagt gatactagt 
      gtagattcc gaagattcg ttcgcttcg gttatattt 
      gtcccgaaa tcggattcc atgtcgccg acgccacta 
      tccccaggt ccaatctcc ccacgggtc aaaacctcc 
      tcagtcccc aactcgccc cgaatcaag caatcgtcc 
      tgccccacc agcccccga atcaagcaa atgcccctg 
      accgtctac cccttaacc aaacaactc agctccccc 
      aagccaaca acctccgat cttctcacc gacaaatca 
      gacagggac cacacggca agtacaaca gcaagcagc 
      aaaaacctt gcacaaaag tactcagta cctcaaatc 
      ccaaaattt agacggact agtctcaac tcccctagt 
      ctaaacacc agtccggaa aaatcccca gtagaaaaa 
      accgaaacg agaaccgag agactgaaa aaaattaaa 
      gaactcctc acgcaaaag cccggcttt ggggcgcga 
      tccaaatcc aactacccc atagtgcgc cactcctcc 
      gtcgccaac ggcaaagtc gaggccgtg gcgaagccg 
      ctccccaaa ctcctaacc ctcgagctc ttcaacccg 
      gaactagac gacaaagac agtgactcc agctgcgtc 
      tcaagcccc gattccatc gacagcgtc ataaacgtg 
      cgcacggac atcaccaga accaagttc aaattccct 
      ccctccaaa cccaaagac ccgaagccg atgagcctc 
      ctcgaggcc gccgccgac gtggccaac accctggac 
      gaagccgtg gagaaagtg atcaagtcc agcccccgc 
      acgaagcgg aagctccag accggcgac gtgctctcc 
      atcatacaa cgacgctac tccagcgac accacgccc 
      ctgctgggg ggcccctcc tgggacgag gagtgccac 
      aagcacctc accgacttc gccgacaag ctcagcgag 
      aagctgctc caagagatc gaccagtat caagagaag 
      agcaaggct ctgaccaca tcgattgac aacatcgac 
      gacccgtac attcatcga ctgagcgag gagttagac 
      gatctgtcc aagttaagt gccgagatt cagaaacag 
      aacgagtat ttggccaag ttgagtgcg agtgatagt 
      ttgtttaat cgagcttgt gcaaagtgt agggaagtc 
      gagtgtaaa tgctacgaa cggaccaaa acgaaagaa 
      aaaactgat acgaaaaaa gtgccagtt agtgagttt 
      ttcagtgtg aaaaatgac gcttttaaa aggtcgcac 
      acgaacgag tcgctgagc gaaacgcca gagtttgca 
      aactgcaag ctctccgtc agcaagacg tccatcagg 
      atgggaacc tccaccgac tcgtgcgat tcggacaaa 
      ggcagtagc acaaactcg atgatttcg agcgtggcg 
      acctcgatc aacttcggg cagtcgctg gagtcggcc 
      gatggggtt gaaagccac aaggacacc aactccatc 
      agcagatac agcgacggg ggaagcacc gcatcgttg 
      gcgtcgtgt cccgaatgg aaaacgatc aaaccgaaa 
      accgaggaa cgattatcg aattcgacc gtcagtctc 
      gagagcgat tcgtcgaag aagcgcgaa aagtcgaca 
      ctgctgagc gatacgtcg caggactcg ctgccttcg 
      gacaatatc ggcggcgaa ataacttat catagatat 
      tatcacgtg tttagagag ggagaactg gaccaattg 
      atagaaaaa tatgtagaa aacctccac attatatcg 
      tcgtattac gaccacgcc agctggtgt ataattgcc 
      gagaaggtg caagtatgg actatctga
    mRNA sequence
    • >TC008156 RA
      cggcaaatg atttccccg aatggactg acgtcagcg 
      gacctcaat gggttaccg gggcaacgg ctgcaagcg 
      tgcaactgc aacctgccc aaaatttcc caaaaatgg 
      gtgaatttg ccgggggtt cccccgagt cgctcgggg 
      cctcccccg gcggattgg gccccattt ggcggcctg 
      attaaaatt gtatgcgtc tcgacgacg tcggcggcg 
      gcgcggcgc aggtccgtg acgtcaaaa ccgcctcca 
      cagttagtc gggcttgga aatacgctg aggatgtat 
      tacaatcgg ggttgttgt ctgttaatt ccgctaaaa 
      gcgtcgcga cctgtgaaa atgtctgtg atagagtgt 
      tacacgttc agttaagca ggcgacacc aaccaacgg 
      gttttgaac aggatttga ttgatcatg ccggtggat 
      gagcgtgtg gcacgaagc gttgctcta gagcaagcc 
      tacgtgcac gacgtctac gaacaattc tacgacaac 
      cccagaagc aagccctgg cccaaagta caacagttt 
      ctgcaggat ctcgaaccc gggtccctg gtctgcgac 
      gtcggttgt ggaaatggg aagtatttg aacgtgaac 
      acttcgata ttcaacatg ggaggcgat aaatcgatg 
      cgattgact gaagtggct cgagacaag gagaacgaa 
      gttatagcc ctcgacaac ttagcgctg cctttccgc 
      gacgaaagc ctcgatgcc gtgctttcc attgccgtc 
      gttcaccat ctggcgaca acggaaaga agaatctgc 
      gccctgagg gagctggcc agagttcta cggatcggc 
      ggccgactc atcatctca gtttgggcc atggaacag 
      agccatcgc aagttcgag tctcaggac gtcctagtt 
      ccctggcac aggcctaag catctaagc acgccctcc 
      ctcgaactc acctccacg actaacacc tcggaagac 
      gactgctta ccgccatat cacgcctac acgcaaact 
      tccgacagc gactccaac agatccgta aagtcaaag 
      ggcattgtg aaaaagcgg gggaagtcc cggcacaag 
      ggccgttcg atcgacccc ggacgtaac tcttctccc 
      agcagttcc agtttatca agtcccaac gaaacttgc 
      tacagtttc gtgcgccgg gctatccag aaattggca 
      ggggggcga agagtgggt gtgcacagg ccctggttt 
      ttggaaagt tggagcact tgtgctaaa gagccgtcg 
      catcaacag cgttacgac catgacgga tgcgaatgc 
      tgcgactgt aatgagaac attcaagat attcctatc 
      gagttgagg agggttgac gaagaggat ttgcctcag 
      aggcggcaa acttttccg tcgtctcag cttgttaac 
      gatatttcc agcaacttg aaatcgaag agtatgacg 
      aatattaag actgttgaa aataataat atagtgagg 
      tcgcaatcg agcacgccg agtgtaccg gaaatcaac 
      gtgttgccg gaaattaag ccgaaggct acgctcgcc 
      aagcccaag ctggttaag cagaaaaaa tcgatctgt 
      gaggaggat gatgaggag gctttggat caaccgact 
      gatatgaaa acgctgatt aataatcta ccggacttt 
      aaggtgttt catgcgagg tatcacaga gggggggtg 
      tttaagcag cggtcgctg aacgaggag ttgatgtcg 
      acggataga ctgaaggag aaggagagg gtgcggcaa 
      aatatccag aaacaagca tctttgaac gaggattta 
      atttatcaa agacacact ttcgactca ttcaaagat 
      tccttcttt tctgtttct acttcgaag cgcttccaa 
      ttaatcaaa acgggtttt acgaataaa attaaaaac 
      tccactaca atagagaaa gtgactggg acatcgctg 
      aaaaacggg ttcgtcagg atgttccaa aactggaaa 
      agttccgac ctgatttcg cccacgatc cccgaaaac 
      gaaacaatc gaccctttg aaaaagcca atagtgatc 
      gaagagaaa aaggacggt tctacgacc gaacggaga 
      cactccaaa gaggacggt tccgactca tccaaagac 
      agtagtttg caaagtgat actagtgta gattccgaa 
      gattcgttc gcttcggtt atatttgtc ccgaaatcg 
      gattccatg tcgccgacg ccactatcc ccaggtcca 
      atctcccca cgggtcaaa acctcctca gtccccaac 
      tcgccccga atcaagcaa tcgtcctgc cccaccagc 
      ccccgaatc aagcaaatg cccctgacc gtctacccc 
      ttaaccaaa caactcagc tcccccaag ccaacaacc 
      tccgatctt ctcaccgac aaatcagac agggaccac 
      acggcaagt acaacagca agcagcaaa aaccttgca 
      caaaagtac tcagtacct caaatccca aaatttaga 
      cggactagt ctcaactcc cctagtcta aacaccagt 
      ccggaaaaa tccccagta gaaaaaacc gaaacgaga 
      accgagaga ctgaaaaaa attaaagaa ctcctcacg 
      caaaagccc ggctttggg gcgcgatcc aaatccaac 
      taccccata gtgcgccac tcctccgtc gccaacggc 
      aaagtcgag gccgtggcg aagccgctc cccaaactc 
      ctaaccctc gagctcttc aacccggaa ctagacgac 
      aaagacagt gactccagc tgcgtctca agccccgat 
      tccatcgac agcgtcata aacgtgcgc acggacatc 
      accagaacc aagttcaaa ttccctccc tccaaaccc 
      aaagacccg aagccgatg agcctcctc gaggccgcc 
      gccgacgtg gccaacacc ctggacgaa gccgtggag 
      aaagtgatc aagtccagc ccccgcacg aagcggaag 
      ctccagacc ggcgacgtg ctctccatc atacaacga 
      cgctactcc agcgacacc acgcccctg ctggggggc 
      ccctcctgg gacgaggag tgccacaag cacctcacc 
      gacttcgcc gacaagctc agcgagaag ctgctccaa 
      gagatcgac cagtatcaa gagaagagc aaggctctg 
      accacatcg attgacaac atcgacgac ccgtacatt 
      catcgactg agcgaggag ttagacgat ctgtccaag 
      ttaagtgcc gagattcag aaacagaac gagtatttg 
      gccaagttg agtgcgagt gatagtttg tttaatcga 
      gcttgtgca aagtgtagg gaagtcgag tgtaaatgc 
      tacgaacgg accaaaacg aaagaaaaa actgatacg 
      aaaaaagtg ccagttagt gagtttttc agtgtgaaa 
      aatgacgct tttaaaagg tcgcacacg aacgagtcg 
      ctgagcgaa acgccagag tttgcaaac tgcaagctc 
      tccgtcagc aagacgtcc atcaggatg ggaacctcc 
      accgactcg tgcgattcg gacaaaggc agtagcaca 
      aactcgatg atttcgagc gtggcgacc tcgatcaac 
      ttcgggcag tcgctggag tcggccgat ggggttgaa 
      agccacaag gacaccaac tccatcagc agatacagc 
      gacggggga agcaccgca tcgttggcg tcgtgtccc 
      gaatggaaa acgatcaaa ccgaaaacc gaggaacga 
      ttatcgaat tcgaccgtc agtctcgag agcgattcg 
      tcgaagaag cgcgaaaag tcgacactg ctgagcgat 
      acgtcgcag gactcgctg ccttcggac aatatcggc 
      ggcgaaata acttatcat agatattat cacgtgttt 
      agagaggga gaactggac caattgata gaaaaatat 
      gtagaaaac ctccacatt atatcgtcg tattacgac 
      cacgccagc tggtgtata attgccgag aaggtgcaa 
      gtatggact atctgacgt gttggaata gaaatgaat 
      caacgtcaa agcacaatg tacataaat gttctcttt 
      aacatagcg tagttgcag gccactcat cttatgaag 
      gttgggctc atttctcac ttcgcaagt agggatttc 
      ctttcggaa ctttaatca attcctggc cttaccttc 
      gactgtata gagttttca taagcctac gtacattaa 
      ctaatgcta aagtcgcct accttgtta gaactaata 
      gaactctat tacagttgc agaatgtgt agagattat 
      cgctactat catggttct gttacattc gtttcatct 
      ttcaagctc aaatcgacc tatagtcag actttgtgt 
      gtttatgta aagtatagc aaattagct cttatattt 
      tttatacaa ttaaagtaa cataattga ttcaaggat 
      catttcggt gttttcgat tctttcacg cgcaacttc 
      ccgtttaaa tatgttcgt tcatcacaa actgggccg 
      aaatcgtgc tttcattac atatcattt acaagttct 
      gtaatttag tttgtctca cgaaatttc ggaacagaa 
      tgatgaact gataagtgt gaactgagc gtctgtttc 
      ccccagcgg gtggcaaac aatcgcatt gaaccggtc 
      gaggaaaat cttcccaca tgtccattt atcgcattc 
      gaaatgctc ctatttttt accgctatg acgtgtgac 
      gaaaagtac atatgacct acgtattga ataataatc 
      ggcagaaat tttcagaca tgagttcat tagcgttag 
      accatggct tctgcacgc aaccgacga acgttgcgt 
      gcaaaagct cgaaactag ttgaagata gctttaaag 
      taaccattg attaatacg ctatttttc atatcttgc 
      ggtatttat gacacaatt tggggctag aataaaccg 
      aacagacaa ataataaat tcctttgat tatattact 
      taatccatt ccgttaccc atcttaaga gagtattaa 
      actttgtgc ctgtcttgt atggtgaag aattttgat 
      acttaaaat aggattcaa cgaaaaact ttgatgtga 
      catgtattt cactctata aatcattaa aaactacaa 
      actaaattt gcatttgac ttgttgtgt ttaataaaa 
      atatactca aatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY