| General information | |||
| TC008156 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5859118..5871357 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008156 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06252 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC008156 RA
MPVDERVAR SVALEQAYV HDVYEQFYD NPRSKPWPK VQQFLQDLE PGSLVCDVG CGNGKYLNV NTSIFNMGG DKSMRLTEV ARDKENEVI ALDNLALPF RDESLDAVL SIAVVHHLA TTERRICAL RELARVLRI GGRLIISVW AMEQSHRKF ESQDVLVPW HRPKHLSTP SLELTSTTN TSEDDCLPP YHAYTQTSD SDSNRSVKS KGIVKKRGK SRHKGRSID PGRNSSPSS SSLSSPNET CYSFVRRAI QKLAGGRRV GVHRPWFLE SWSTCAKEP SHQQRYDHD GCECCDCNE NIQDIPIEL RRVDEEDLP QRRQTFPSS QLVNDISSN LKSKSMTNI KTVENNNIV RSQSSTPSV PEINVLPEI KPKATLAKP KLVKQKKSI CEEDDEEAL DQPTDMKTL INNLPDFKV FHARYHRGG VFKQRSLNE ELMSTDRLK EKERVRQNI QKQASLNED LIYQRHTFD SFKDSFFSV STSKRFQLI KTGFTNKIK NSTTIEKVT GTSLKNGFV RMFQNWKSS DLISPTIPE NETIDPLKK PIVIEEKKD GSTTERRHS KEDGSDSSK DSSLQSDTS VDSEDSFAS VIFVPKSDS MSPTPLSPG PISPRVKTS SVPNSPRIK QSSCPTSPR IKQMPLTVY PLTKQLSSP KPTTSDLLT DKSDRDHTA STTASSKNL AQKYSVPQI PKFRRTSLN SPSLNTSPE KSPVEKTET RTERLKKIK ELLTQKPGF GARSKSNYP IVRHSSVAN GKVEAVAKP LPKLLTLEL FNPELDDKD SDSSCVSSP DSIDSVINV RTDITRTKF KFPPSKPKD PKPMSLLEA AADVANTLD EAVEKVIKS SPRTKRKLQ TGDVLSIIQ RRYSSDTTP LLGGPSWDE ECHKHLTDF ADKLSEKLL QEIDQYQEK SKALTTSID NIDDPYIHR LSEELDDLS KLSAEIQKQ NEYLAKLSA SDSLFNRAC AKCREVECK CYERTKTKE KTDTKKVPV SEFFSVKND AFKRSHTNE SLSETPEFA NCKLSVSKT SIRMGTSTD SCDSDKGSS TNSMISSVA TSINFGQSL ESADGVESH KDTNSISRY SDGGSTASL ASCPEWKTI KPKTEERLS NSTVSLESD SSKKREKST LLSDTSQDS LPSDNIGGE ITYHRYYHV FREGELDQL IEKYVENLH IISSYYDHA SWCIIAEKV QVWTI
- >TC008156 RA
atgccggtg gatgagcgt gtggcacga agcgttgct ctagagcaa gcctacgtg cacgacgtc tacgaacaa ttctacgac aaccccaga agcaagccc tggcccaaa gtacaacag tttctgcag gatctcgaa cccgggtcc ctggtctgc gacgtcggt tgtggaaat gggaagtat ttgaacgtg aacacttcg atattcaac atgggaggc gataaatcg atgcgattg actgaagtg gctcgagac aaggagaac gaagttata gccctcgac aacttagcg ctgcctttc cgcgacgaa agcctcgat gccgtgctt tccattgcc gtcgttcac catctggcg acaacggaa agaagaatc tgcgccctg agggagctg gccagagtt ctacggatc ggcggccga ctcatcatc tcagtttgg gccatggaa cagagccat cgcaagttc gagtctcag gacgtccta gttccctgg cacaggcct aagcatcta agcacgccc tccctcgaa ctcacctcc acgactaac acctcggaa gacgactgc ttaccgcca tatcacgcc tacacgcaa acttccgac agcgactcc aacagatcc gtaaagtca aagggcatt gtgaaaaag cgggggaag tcccggcac aagggccgt tcgatcgac cccggacgt aactcttct cccagcagt tccagttta tcaagtccc aacgaaact tgctacagt ttcgtgcgc cgggctatc cagaaattg gcagggggg cgaagagtg ggtgtgcac aggccctgg tttttggaa agttggagc acttgtgct aaagagccg tcgcatcaa cagcgttac gaccatgac ggatgcgaa tgctgcgac tgtaatgag aacattcaa gatattcct atcgagttg aggagggtt gacgaagag gatttgcct cagaggcgg caaactttt ccgtcgtct cagcttgtt aacgatatt tccagcaac ttgaaatcg aagagtatg acgaatatt aagactgtt gaaaataat aatatagtg aggtcgcaa tcgagcacg ccgagtgta ccggaaatc aacgtgttg ccggaaatt aagccgaag gctacgctc gccaagccc aagctggtt aagcagaaa aaatcgatc tgtgaggag gatgatgag gaggctttg gatcaaccg actgatatg aaaacgctg attaataat ctaccggac tttaaggtg tttcatgcg aggtatcac agagggggg gtgtttaag cagcggtcg ctgaacgag gagttgatg tcgacggat agactgaag gagaaggag agggtgcgg caaaatatc cagaaacaa gcatctttg aacgaggat ttaatttat caaagacac actttcgac tcattcaaa gattccttc ttttctgtt tctacttcg aagcgcttc caattaatc aaaacgggt tttacgaat aaaattaaa aactccact acaatagag aaagtgact gggacatcg ctgaaaaac gggttcgtc aggatgttc caaaactgg aaaagttcc gacctgatt tcgcccacg atccccgaa aacgaaaca atcgaccct ttgaaaaag ccaatagtg atcgaagag aaaaaggac ggttctacg accgaacgg agacactcc aaagaggac ggttccgac tcatccaaa gacagtagt ttgcaaagt gatactagt gtagattcc gaagattcg ttcgcttcg gttatattt gtcccgaaa tcggattcc atgtcgccg acgccacta tccccaggt ccaatctcc ccacgggtc aaaacctcc tcagtcccc aactcgccc cgaatcaag caatcgtcc tgccccacc agcccccga atcaagcaa atgcccctg accgtctac cccttaacc aaacaactc agctccccc aagccaaca acctccgat cttctcacc gacaaatca gacagggac cacacggca agtacaaca gcaagcagc aaaaacctt gcacaaaag tactcagta cctcaaatc ccaaaattt agacggact agtctcaac tcccctagt ctaaacacc agtccggaa aaatcccca gtagaaaaa accgaaacg agaaccgag agactgaaa aaaattaaa gaactcctc acgcaaaag cccggcttt ggggcgcga tccaaatcc aactacccc atagtgcgc cactcctcc gtcgccaac ggcaaagtc gaggccgtg gcgaagccg ctccccaaa ctcctaacc ctcgagctc ttcaacccg gaactagac gacaaagac agtgactcc agctgcgtc tcaagcccc gattccatc gacagcgtc ataaacgtg cgcacggac atcaccaga accaagttc aaattccct ccctccaaa cccaaagac ccgaagccg atgagcctc ctcgaggcc gccgccgac gtggccaac accctggac gaagccgtg gagaaagtg atcaagtcc agcccccgc acgaagcgg aagctccag accggcgac gtgctctcc atcatacaa cgacgctac tccagcgac accacgccc ctgctgggg ggcccctcc tgggacgag gagtgccac aagcacctc accgacttc gccgacaag ctcagcgag aagctgctc caagagatc gaccagtat caagagaag agcaaggct ctgaccaca tcgattgac aacatcgac gacccgtac attcatcga ctgagcgag gagttagac gatctgtcc aagttaagt gccgagatt cagaaacag aacgagtat ttggccaag ttgagtgcg agtgatagt ttgtttaat cgagcttgt gcaaagtgt agggaagtc gagtgtaaa tgctacgaa cggaccaaa acgaaagaa aaaactgat acgaaaaaa gtgccagtt agtgagttt ttcagtgtg aaaaatgac gcttttaaa aggtcgcac acgaacgag tcgctgagc gaaacgcca gagtttgca aactgcaag ctctccgtc agcaagacg tccatcagg atgggaacc tccaccgac tcgtgcgat tcggacaaa ggcagtagc acaaactcg atgatttcg agcgtggcg acctcgatc aacttcggg cagtcgctg gagtcggcc gatggggtt gaaagccac aaggacacc aactccatc agcagatac agcgacggg ggaagcacc gcatcgttg gcgtcgtgt cccgaatgg aaaacgatc aaaccgaaa accgaggaa cgattatcg aattcgacc gtcagtctc gagagcgat tcgtcgaag aagcgcgaa aagtcgaca ctgctgagc gatacgtcg caggactcg ctgccttcg gacaatatc ggcggcgaa ataacttat catagatat tatcacgtg tttagagag ggagaactg gaccaattg atagaaaaa tatgtagaa aacctccac attatatcg tcgtattac gaccacgcc agctggtgt ataattgcc gagaaggtg caagtatgg actatctga
- >TC008156 RA
cggcaaatg atttccccg aatggactg acgtcagcg gacctcaat gggttaccg gggcaacgg ctgcaagcg tgcaactgc aacctgccc aaaatttcc caaaaatgg gtgaatttg ccgggggtt cccccgagt cgctcgggg cctcccccg gcggattgg gccccattt ggcggcctg attaaaatt gtatgcgtc tcgacgacg tcggcggcg gcgcggcgc aggtccgtg acgtcaaaa ccgcctcca cagttagtc gggcttgga aatacgctg aggatgtat tacaatcgg ggttgttgt ctgttaatt ccgctaaaa gcgtcgcga cctgtgaaa atgtctgtg atagagtgt tacacgttc agttaagca ggcgacacc aaccaacgg gttttgaac aggatttga ttgatcatg ccggtggat gagcgtgtg gcacgaagc gttgctcta gagcaagcc tacgtgcac gacgtctac gaacaattc tacgacaac cccagaagc aagccctgg cccaaagta caacagttt ctgcaggat ctcgaaccc gggtccctg gtctgcgac gtcggttgt ggaaatggg aagtatttg aacgtgaac acttcgata ttcaacatg ggaggcgat aaatcgatg cgattgact gaagtggct cgagacaag gagaacgaa gttatagcc ctcgacaac ttagcgctg cctttccgc gacgaaagc ctcgatgcc gtgctttcc attgccgtc gttcaccat ctggcgaca acggaaaga agaatctgc gccctgagg gagctggcc agagttcta cggatcggc ggccgactc atcatctca gtttgggcc atggaacag agccatcgc aagttcgag tctcaggac gtcctagtt ccctggcac aggcctaag catctaagc acgccctcc ctcgaactc acctccacg actaacacc tcggaagac gactgctta ccgccatat cacgcctac acgcaaact tccgacagc gactccaac agatccgta aagtcaaag ggcattgtg aaaaagcgg gggaagtcc cggcacaag ggccgttcg atcgacccc ggacgtaac tcttctccc agcagttcc agtttatca agtcccaac gaaacttgc tacagtttc gtgcgccgg gctatccag aaattggca ggggggcga agagtgggt gtgcacagg ccctggttt ttggaaagt tggagcact tgtgctaaa gagccgtcg catcaacag cgttacgac catgacgga tgcgaatgc tgcgactgt aatgagaac attcaagat attcctatc gagttgagg agggttgac gaagaggat ttgcctcag aggcggcaa acttttccg tcgtctcag cttgttaac gatatttcc agcaacttg aaatcgaag agtatgacg aatattaag actgttgaa aataataat atagtgagg tcgcaatcg agcacgccg agtgtaccg gaaatcaac gtgttgccg gaaattaag ccgaaggct acgctcgcc aagcccaag ctggttaag cagaaaaaa tcgatctgt gaggaggat gatgaggag gctttggat caaccgact gatatgaaa acgctgatt aataatcta ccggacttt aaggtgttt catgcgagg tatcacaga gggggggtg tttaagcag cggtcgctg aacgaggag ttgatgtcg acggataga ctgaaggag aaggagagg gtgcggcaa aatatccag aaacaagca tctttgaac gaggattta atttatcaa agacacact ttcgactca ttcaaagat tccttcttt tctgtttct acttcgaag cgcttccaa ttaatcaaa acgggtttt acgaataaa attaaaaac tccactaca atagagaaa gtgactggg acatcgctg aaaaacggg ttcgtcagg atgttccaa aactggaaa agttccgac ctgatttcg cccacgatc cccgaaaac gaaacaatc gaccctttg aaaaagcca atagtgatc gaagagaaa aaggacggt tctacgacc gaacggaga cactccaaa gaggacggt tccgactca tccaaagac agtagtttg caaagtgat actagtgta gattccgaa gattcgttc gcttcggtt atatttgtc ccgaaatcg gattccatg tcgccgacg ccactatcc ccaggtcca atctcccca cgggtcaaa acctcctca gtccccaac tcgccccga atcaagcaa tcgtcctgc cccaccagc ccccgaatc aagcaaatg cccctgacc gtctacccc ttaaccaaa caactcagc tcccccaag ccaacaacc tccgatctt ctcaccgac aaatcagac agggaccac acggcaagt acaacagca agcagcaaa aaccttgca caaaagtac tcagtacct caaatccca aaatttaga cggactagt ctcaactcc cctagtcta aacaccagt ccggaaaaa tccccagta gaaaaaacc gaaacgaga accgagaga ctgaaaaaa attaaagaa ctcctcacg caaaagccc ggctttggg gcgcgatcc aaatccaac taccccata gtgcgccac tcctccgtc gccaacggc aaagtcgag gccgtggcg aagccgctc cccaaactc ctaaccctc gagctcttc aacccggaa ctagacgac aaagacagt gactccagc tgcgtctca agccccgat tccatcgac agcgtcata aacgtgcgc acggacatc accagaacc aagttcaaa ttccctccc tccaaaccc aaagacccg aagccgatg agcctcctc gaggccgcc gccgacgtg gccaacacc ctggacgaa gccgtggag aaagtgatc aagtccagc ccccgcacg aagcggaag ctccagacc ggcgacgtg ctctccatc atacaacga cgctactcc agcgacacc acgcccctg ctggggggc ccctcctgg gacgaggag tgccacaag cacctcacc gacttcgcc gacaagctc agcgagaag ctgctccaa gagatcgac cagtatcaa gagaagagc aaggctctg accacatcg attgacaac atcgacgac ccgtacatt catcgactg agcgaggag ttagacgat ctgtccaag ttaagtgcc gagattcag aaacagaac gagtatttg gccaagttg agtgcgagt gatagtttg tttaatcga gcttgtgca aagtgtagg gaagtcgag tgtaaatgc tacgaacgg accaaaacg aaagaaaaa actgatacg aaaaaagtg ccagttagt gagtttttc agtgtgaaa aatgacgct tttaaaagg tcgcacacg aacgagtcg ctgagcgaa acgccagag tttgcaaac tgcaagctc tccgtcagc aagacgtcc atcaggatg ggaacctcc accgactcg tgcgattcg gacaaaggc agtagcaca aactcgatg atttcgagc gtggcgacc tcgatcaac ttcgggcag tcgctggag tcggccgat ggggttgaa agccacaag gacaccaac tccatcagc agatacagc gacggggga agcaccgca tcgttggcg tcgtgtccc gaatggaaa acgatcaaa ccgaaaacc gaggaacga ttatcgaat tcgaccgtc agtctcgag agcgattcg tcgaagaag cgcgaaaag tcgacactg ctgagcgat acgtcgcag gactcgctg ccttcggac aatatcggc ggcgaaata acttatcat agatattat cacgtgttt agagaggga gaactggac caattgata gaaaaatat gtagaaaac ctccacatt atatcgtcg tattacgac cacgccagc tggtgtata attgccgag aaggtgcaa gtatggact atctgacgt gttggaata gaaatgaat caacgtcaa agcacaatg tacataaat gttctcttt aacatagcg tagttgcag gccactcat cttatgaag gttgggctc atttctcac ttcgcaagt agggatttc ctttcggaa ctttaatca attcctggc cttaccttc gactgtata gagttttca taagcctac gtacattaa ctaatgcta aagtcgcct accttgtta gaactaata gaactctat tacagttgc agaatgtgt agagattat cgctactat catggttct gttacattc gtttcatct ttcaagctc aaatcgacc tatagtcag actttgtgt gtttatgta aagtatagc aaattagct cttatattt tttatacaa ttaaagtaa cataattga ttcaaggat catttcggt gttttcgat tctttcacg cgcaacttc ccgtttaaa tatgttcgt tcatcacaa actgggccg aaatcgtgc tttcattac atatcattt acaagttct gtaatttag tttgtctca cgaaatttc ggaacagaa tgatgaact gataagtgt gaactgagc gtctgtttc ccccagcgg gtggcaaac aatcgcatt gaaccggtc gaggaaaat cttcccaca tgtccattt atcgcattc gaaatgctc ctatttttt accgctatg acgtgtgac gaaaagtac atatgacct acgtattga ataataatc ggcagaaat tttcagaca tgagttcat tagcgttag accatggct tctgcacgc aaccgacga acgttgcgt gcaaaagct cgaaactag ttgaagata gctttaaag taaccattg attaatacg ctatttttc atatcttgc ggtatttat gacacaatt tggggctag aataaaccg aacagacaa ataataaat tcctttgat tatattact taatccatt ccgttaccc atcttaaga gagtattaa actttgtgc ctgtcttgt atggtgaag aattttgat acttaaaat aggattcaa cgaaaaact ttgatgtga catgtattt cactctata aatcattaa aaactacaa actaaattt gcatttgac ttgttgtgt ttaataaaa atatactca aatg
