Status
Please wait ...
Gene information
General information
TC032822
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008141,TC008140 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5625376..5646464
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032822
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06250

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032822 RA
      MSGRPRTTS FAEGNKQPL NPPLGGMKI SSKDGSKVT 
TVVATPGQG PDRPQEVSY TDTKVIGNG SFGVVYQAK 
LCETNEFVA IKKVLQDKR FKNRELQIM RKLEHCNIV 
KLKYFFYSS GDKKDEVYL NLVLEYIPE TVYRVARHY 
SKSKQTIPI IFIKLYMYQ LFRSLAYIH SLGICHRDI 
KPQNLLLDP GTGILKLCD FGSAKHLVK GEPNVSYIC 
SRYYRAPEL IFGAIDYTT KIDVWSAGC VLAELLLGQ 
PIFPGDSGV DQLVEIIKV LGTPTKEQI KEMNPNYTE 
FKFPQIKSH PWQQVFRAR TPPEAIELV ARLLEYTPS 
SRISPLQAC AHAFFNELR DPNTRLPTN KELPPLFNF 
TEQELSIQP SLNSILIPR GVQEAANSP QDGAASSGA 
TDAADTTAA PVQATAAGI P
    • >TC032822 RB
      MSGRPRTTS FAEGNKQPL NPPLGGMKI SSLLTDAKT 
FRDGNDWGS GGGGAPETA ATARGAPIA RPAGLPIRG 
NSSKLTTIF RIFADDSKV ASDANYVVK KQCECKKLQ 
SVLPEKNKN ARPKYGRSV SVCDLSSDK HKSTKKSAT 
LGSVPAIDG LGKSQKTTG YSRLLYCWR KLKRSLSSS 
GSKEKGREV FDDDQYVIA STKLKSGKD GSKVTTVVA 
TPGQGPDRP QEVSYTDTK VIGNGSFGV VYQAKLCET 
NEFVAIKKV LQDKRFKNR ELQIMRKLE HCNIVKLKY 
FFYSSGDKK DEVYLNLVL EYIPETVYR VARHYSKSK 
QTIPIIFIK LYMYQLFRS LAYIHSLGI CHRDIKPQN 
LLLDPGTGI LKLCDFGSA KHLVKGEPN VSYICSRYY 
RAPELIFGA IDYTTKIDV WSAGCVLAE LLLGQPIFP 
GDSGVDQLV EIIKVLGTP TKEQIKEMN PNYTEFKFP 
QIKSHPWQQ VFRARTPPE AIELVARLL EYTPSSRIS 
PLQACAHAF FNELRDPNT RLPTNKELP PLFNFTEQE 
LSIQPSLNS ILIPRGVQE AANSPQDGA ASSGATDAA 
DTTAAPVQA TAAGIP
    CDS sequence
    • >TC032822 RA
      atgagcgga cgccccaga accacgtcc ttcgccgag 
ggcaacaag cagccgctg aatcccccc ctgggaggc 
atgaagatt agcagtaag gacggtagc aaagttact 
acggtggta gcaacacct ggacaaggt cccgacaga 
ccgcaagaa gtctcatac actgacacc aaagttatc 
ggcaatggc agcttcgga gtcgtctat caggccaag 
ctgtgcgaa accaacgag ttcgtcgcc atcaaaaaa 
gtcctccaa gacaagagg tttaagaac cgggaactt 
caaataatg aggaaatta gaacattgt aatatagtt 
aaacttaaa tatttcttt tactccagt ggagataag 
aaggacgaa gtgtacctg aatttggtg ttggaatac 
atcccagaa acggtatac agagtagcg agacattac 
agcaaatca aagcaaaca atcccgata atttttatc 
aagttgtat atgtaccag ttattccga tcgttggcg 
tacatacac tcgttgggt atctgtcat cgggacatt 
aaaccgcaa aatcttctg ctggatcca ggcactggc 
atattgaag ttgtgcgac ttcggcagc gctaaacac 
ctcgtcaaa ggcgaaccg aacgtctcg tacatttgc 
agtcgttat tacagagca ccggaactg atattcggc 
gctatcgac tacactacg aagatcgac gtgtggagt 
gcgggctgt gtcctggcc gaattactg ctgggccag 
ccgatattc cccggagat tccggtgta gaccaatta 
gtagaaatt atcaaagtt ttaggcacg ccaactaaa 
gaacagatc aaggagatg aatccgaat tatacagag 
ttcaagttt ccgcagatc aagtcgcat ccttggcaa 
caggtattc cgagcacgg actccccca gaagcgatc 
gagttagtg gccagactt ttggagtac actccttca 
tcacggatc agtccattg caggcttgt gctcacgca 
ttcttcaac gagctccgg gaccccaac acccgcttg 
cccacaaat aaagaactg ccccctcta ttcaacttc 
acggaacaa gagttgagt attcaacct tcattgaat 
tccattctg ataccgcga ggggtgcag gaggccgcc 
aacagtccg caagacggg gccgcgtcg tcgggggcg 
acggacgcc gccgacacg acggcagcc cccgtgcaa 
gcgaccgcc gccggaata ccttaa
    • >TC032822 RB
      atgagcgga cgccccaga accacgtcc ttcgccgag 
ggcaacaag cagccgctg aatcccccc ctgggaggc 
atgaagatt agcagtcta ctaactgac gcaaaaacg 
tttcgggac ggtaacgat tggggctcc gggggcggc 
ggcgccccc gagacggcc gccaccgcg cggggcgcg 
cccattgcg cgcccagcg ggccttccc atccgcgga 
aattcctcc aaattaacg acgattttc cgaattttt 
gccgacgac tctaaagtc gcaagtgat gcgaattat 
gtggttaaa aaacagtgc gagtgtaag aaattgcaa 
agtgttttg cccgaaaag aacaaaaac gcgagacct 
aagtacggc aggtcagtt tctgtgtgc gatttatct 
tcggacaag cacaagtcg accaagaaa agtgcgact 
ttggggtcg gtgccggcg atcgatgga ctggggaaa 
agccaaaaa acgacgggc tactccagg ttgttgtat 
tgctggagg aagttgaag aggtcgctg tcgagctcg 
ggaagtaaa gaaaaaggc cgtgaagtg ttcgacgac 
gatcaatac gtcattgct tcgaccaaa ctcaagtca 
ggtaaggac ggtagcaaa gttactacg gtggtagca 
acacctgga caaggtccc gacagaccg caagaagtc 
tcatacact gacaccaaa gttatcggc aatggcagc 
ttcggagtc gtctatcag gccaagctg tgcgaaacc 
aacgagttc gtcgccatc aaaaaagtc ctccaagac 
aagaggttt aagaaccgg gaacttcaa ataatgagg 
aaattagaa cattgtaat atagttaaa cttaaatat 
ttcttttac tccagtgga gataagaag gacgaagtg 
tacctgaat ttggtgttg gaatacatc ccagaaacg 
gtatacaga gtagcgaga cattacagc aaatcaaag 
caaacaatc ccgataatt tttatcaag ttgtatatg 
taccagtta ttccgatcg ttggcgtac atacactcg 
ttgggtatc tgtcatcgg gacattaaa ccgcaaaat 
cttctgctg gatccaggc actggcata ttgaagttg 
tgcgacttc ggcagcgct aaacacctc gtcaaaggc 
gaaccgaac gtctcgtac atttgcagt cgttattac 
agagcaccg gaactgata ttcggcgct atcgactac 
actacgaag atcgacgtg tggagtgcg ggctgtgtc 
ctggccgaa ttactgctg ggccagccg atattcccc 
ggagattcc ggtgtagac caattagta gaaattatc 
aaagtttta ggcacgcca actaaagaa cagatcaag 
gagatgaat ccgaattat acagagttc aagtttccg 
cagatcaag tcgcatcct tggcaacag gtattccga 
gcacggact cccccagaa gcgatcgag ttagtggcc 
agacttttg gagtacact ccttcatca cggatcagt 
ccattgcag gcttgtgct cacgcattc ttcaacgag 
ctccgggac cccaacacc cgcttgccc acaaataaa 
gaactgccc cctctattc aacttcacg gaacaagag 
ttgagtatt caaccttca ttgaattcc attctgata 
ccgcgaggg gtgcaggag gccgccaac agtccgcaa 
gacggggcc gcgtcgtcg ggggcgacg gacgccgcc 
gacacgacg gcagccccc gtgcaagcg accgccgcc 
ggaatacct taa
    mRNA sequence
    • >TC032822 RA
      cgtttgagg tgcgtgaga aggacaggt cgtaagaag 
ggtgtcagc aagaaatgt gataggtct gcgaataat 
cgacttagg agaatttgc aatgagcgg acgccccag 
aaccacgtc cttcgccga gggcaacaa gcagccgct 
gaatccccc cctgggagg catgaagat tagcagtaa 
ggacggtag caaagttac tacggtggt agcaacacc 
tggacaagg tcccgacag accgcaaga agtctcata 
cactgacac caaagttat cggcaatgg cagcttcgg 
agtcgtcta tcaggccaa gctgtgcga aaccaacga 
gttcgtcgc catcaaaaa agtcctcca agacaagag 
gtttaagaa ccgggaact tcaaataat gaggaaatt 
agaacattg taatatagt taaacttaa atatttctt 
ttactccag tggagataa gaaggacga agtgtacct 
gaatttggt gttggaata catcccaga aacggtata 
cagagtagc gagacatta cagcaaatc aaagcaaac 
aatcccgat aatttttat caagttgta tatgtacca 
gttattccg atcgttggc gtacataca ctcgttggg 
tatctgtca tcgggacat taaaccgca aaatcttct 
gctggatcc aggcactgg catattgaa gttgtgcga 
cttcggcag cgctaaaca cctcgtcaa aggcgaacc 
gaacgtctc gtacatttg cagtcgtta ttacagagc 
accggaact gatattcgg cgctatcga ctacactac 
gaagatcga cgtgtggag tgcgggctg tgtcctggc 
cgaattact gctgggcca gccgatatt ccccggaga 
ttccggtgt agaccaatt agtagaaat tatcaaagt 
tttaggcac gccaactaa agaacagat caaggagat 
gaatccgaa ttatacaga gttcaagtt tccgcagat 
caagtcgca tccttggca acaggtatt ccgagcacg 
gactccccc agaagcgat cgagttagt ggccagact 
tttggagta cactccttc atcacggat cagtccatt 
gcaggcttg tgctcacgc attcttcaa cgagctccg 
ggaccccaa cacccgctt gcccacaaa taaagaact 
gccccctct attcaactt cacggaaca agagttgag 
tattcaacc ttcattgaa ttccattct gataccgcg 
aggggtgca ggaggccgc caacagtcc gcaagacgg 
ggccgcgtc gtcgggggc gacggacgc cgccgacac 
gacggcagc ccccgtgca agcgaccgc cgccggaat 
accttaagt agttcaatc ctccaaggg aagggcccc 
tttcgctcg tcccatttc tccctttat ctagtaagc 
gatctgtgt gagattgtt gattgggac agctgtact 
atatttaat atacgtgcg tttgtaca
    • >TC032822 RB
      cgtttgagg tgcgtgaga aggacaggt cgtaagaag 
ggtgtcagc aagaaatgt gataggtct gcgaataat 
cgacttagg agaatttgc aatgagcgg acgccccag 
aaccacgtc cttcgccga gggcaacaa gcagccgct 
gaatccccc cctgggagg catgaagat tagcagtct 
actaactga cgcaaaaac gtttcggga cggtaacga 
ttggggctc cgggggcgg cggcgcccc cgagacggc 
cgccaccgc gcggggcgc gcccattgc gcgcccagc 
gggccttcc catccgcgg aaattcctc caaattaac 
gacgatttt ccgaatttt tgccgacga ctctaaagt 
cgcaagtga tgcgaatta tgtggttaa aaaacagtg 
cgagtgtaa gaaattgca aagtgtttt gcccgaaaa 
gaacaaaaa cgcgagacc taagtacgg caggtcagt 
ttctgtgtg cgatttatc ttcggacaa gcacaagtc 
gaccaagaa aagtgcgac tttggggtc ggtgccggc 
gatcgatgg actggggaa aagccaaaa aacgacggg 
ctactccag gttgttgta ttgctggag gaagttgaa 
gaggtcgct gtcgagctc gggaagtaa agaaaaagg 
ccgtgaagt gttcgacga cgatcaata cgtcattgc 
ttcgaccaa actcaagtc aggtaagga cggtagcaa 
agttactac ggtggtagc aacacctgg acaaggtcc 
cgacagacc gcaagaagt ctcatacac tgacaccaa 
agttatcgg caatggcag cttcggagt cgtctatca 
ggccaagct gtgcgaaac caacgagtt cgtcgccat 
caaaaaagt cctccaaga caagaggtt taagaaccg 
ggaacttca aataatgag gaaattaga acattgtaa 
tatagttaa acttaaata tttctttta ctccagtgg 
agataagaa ggacgaagt gtacctgaa tttggtgtt 
ggaatacat cccagaaac ggtatacag agtagcgag 
acattacag caaatcaaa gcaaacaat cccgataat 
ttttatcaa gttgtatat gtaccagtt attccgatc 
gttggcgta catacactc gttgggtat ctgtcatcg 
ggacattaa accgcaaaa tcttctgct ggatccagg 
cactggcat attgaagtt gtgcgactt cggcagcgc 
taaacacct cgtcaaagg cgaaccgaa cgtctcgta 
catttgcag tcgttatta cagagcacc ggaactgat 
attcggcgc tatcgacta cactacgaa gatcgacgt 
gtggagtgc gggctgtgt cctggccga attactgct 
gggccagcc gatattccc cggagattc cggtgtaga 
ccaattagt agaaattat caaagtttt aggcacgcc 
aactaaaga acagatcaa ggagatgaa tccgaatta 
tacagagtt caagtttcc gcagatcaa gtcgcatcc 
ttggcaaca ggtattccg agcacggac tcccccaga 
agcgatcga gttagtggc cagactttt ggagtacac 
tccttcatc acggatcag tccattgca ggcttgtgc 
tcacgcatt cttcaacga gctccggga ccccaacac 
ccgcttgcc cacaaataa agaactgcc ccctctatt 
caacttcac ggaacaaga gttgagtat tcaaccttc 
attgaattc cattctgat accgcgagg ggtgcagga 
ggccgccaa cagtccgca agacggggc cgcgtcgtc 
gggggcgac ggacgccgc cgacacgac ggcagcccc 
cgtgcaagc gaccgccgc cggaatacc ttaagtagt 
tcaatcctc caagggaag ggccccttt cgctcgtcc 
catttctcc ctttatcta gtaagcgat ctgtgtgag 
attgttgat tgggacagc tgtactata tttaatata 
cgtgcgttt gtaca

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY