| General information | |||
| TC032822
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5625376..5646464 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032822 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06250 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC032822 RA
MSGRPRTTS FAEGNKQPL NPPLGGMKI SSKDGSKVT TVVATPGQG PDRPQEVSY TDTKVIGNG SFGVVYQAK LCETNEFVA IKKVLQDKR FKNRELQIM RKLEHCNIV KLKYFFYSS GDKKDEVYL NLVLEYIPE TVYRVARHY SKSKQTIPI IFIKLYMYQ LFRSLAYIH SLGICHRDI KPQNLLLDP GTGILKLCD FGSAKHLVK GEPNVSYIC SRYYRAPEL IFGAIDYTT KIDVWSAGC VLAELLLGQ PIFPGDSGV DQLVEIIKV LGTPTKEQI KEMNPNYTE FKFPQIKSH PWQQVFRAR TPPEAIELV ARLLEYTPS SRISPLQAC AHAFFNELR DPNTRLPTN KELPPLFNF TEQELSIQP SLNSILIPR GVQEAANSP QDGAASSGA TDAADTTAA PVQATAAGI P
- >TC032822 RB
MSGRPRTTS FAEGNKQPL NPPLGGMKI SSLLTDAKT FRDGNDWGS GGGGAPETA ATARGAPIA RPAGLPIRG NSSKLTTIF RIFADDSKV ASDANYVVK KQCECKKLQ SVLPEKNKN ARPKYGRSV SVCDLSSDK HKSTKKSAT LGSVPAIDG LGKSQKTTG YSRLLYCWR KLKRSLSSS GSKEKGREV FDDDQYVIA STKLKSGKD GSKVTTVVA TPGQGPDRP QEVSYTDTK VIGNGSFGV VYQAKLCET NEFVAIKKV LQDKRFKNR ELQIMRKLE HCNIVKLKY FFYSSGDKK DEVYLNLVL EYIPETVYR VARHYSKSK QTIPIIFIK LYMYQLFRS LAYIHSLGI CHRDIKPQN LLLDPGTGI LKLCDFGSA KHLVKGEPN VSYICSRYY RAPELIFGA IDYTTKIDV WSAGCVLAE LLLGQPIFP GDSGVDQLV EIIKVLGTP TKEQIKEMN PNYTEFKFP QIKSHPWQQ VFRARTPPE AIELVARLL EYTPSSRIS PLQACAHAF FNELRDPNT RLPTNKELP PLFNFTEQE LSIQPSLNS ILIPRGVQE AANSPQDGA ASSGATDAA DTTAAPVQA TAAGIP
- >TC032822 RA
atgagcgga cgccccaga accacgtcc ttcgccgag ggcaacaag cagccgctg aatcccccc ctgggaggc atgaagatt agcagtaag gacggtagc aaagttact acggtggta gcaacacct ggacaaggt cccgacaga ccgcaagaa gtctcatac actgacacc aaagttatc ggcaatggc agcttcgga gtcgtctat caggccaag ctgtgcgaa accaacgag ttcgtcgcc atcaaaaaa gtcctccaa gacaagagg tttaagaac cgggaactt caaataatg aggaaatta gaacattgt aatatagtt aaacttaaa tatttcttt tactccagt ggagataag aaggacgaa gtgtacctg aatttggtg ttggaatac atcccagaa acggtatac agagtagcg agacattac agcaaatca aagcaaaca atcccgata atttttatc aagttgtat atgtaccag ttattccga tcgttggcg tacatacac tcgttgggt atctgtcat cgggacatt aaaccgcaa aatcttctg ctggatcca ggcactggc atattgaag ttgtgcgac ttcggcagc gctaaacac ctcgtcaaa ggcgaaccg aacgtctcg tacatttgc agtcgttat tacagagca ccggaactg atattcggc gctatcgac tacactacg aagatcgac gtgtggagt gcgggctgt gtcctggcc gaattactg ctgggccag ccgatattc cccggagat tccggtgta gaccaatta gtagaaatt atcaaagtt ttaggcacg ccaactaaa gaacagatc aaggagatg aatccgaat tatacagag ttcaagttt ccgcagatc aagtcgcat ccttggcaa caggtattc cgagcacgg actccccca gaagcgatc gagttagtg gccagactt ttggagtac actccttca tcacggatc agtccattg caggcttgt gctcacgca ttcttcaac gagctccgg gaccccaac acccgcttg cccacaaat aaagaactg ccccctcta ttcaacttc acggaacaa gagttgagt attcaacct tcattgaat tccattctg ataccgcga ggggtgcag gaggccgcc aacagtccg caagacggg gccgcgtcg tcgggggcg acggacgcc gccgacacg acggcagcc cccgtgcaa gcgaccgcc gccggaata ccttaa
- >TC032822 RB
atgagcgga cgccccaga accacgtcc ttcgccgag ggcaacaag cagccgctg aatcccccc ctgggaggc atgaagatt agcagtcta ctaactgac gcaaaaacg tttcgggac ggtaacgat tggggctcc gggggcggc ggcgccccc gagacggcc gccaccgcg cggggcgcg cccattgcg cgcccagcg ggccttccc atccgcgga aattcctcc aaattaacg acgattttc cgaattttt gccgacgac tctaaagtc gcaagtgat gcgaattat gtggttaaa aaacagtgc gagtgtaag aaattgcaa agtgttttg cccgaaaag aacaaaaac gcgagacct aagtacggc aggtcagtt tctgtgtgc gatttatct tcggacaag cacaagtcg accaagaaa agtgcgact ttggggtcg gtgccggcg atcgatgga ctggggaaa agccaaaaa acgacgggc tactccagg ttgttgtat tgctggagg aagttgaag aggtcgctg tcgagctcg ggaagtaaa gaaaaaggc cgtgaagtg ttcgacgac gatcaatac gtcattgct tcgaccaaa ctcaagtca ggtaaggac ggtagcaaa gttactacg gtggtagca acacctgga caaggtccc gacagaccg caagaagtc tcatacact gacaccaaa gttatcggc aatggcagc ttcggagtc gtctatcag gccaagctg tgcgaaacc aacgagttc gtcgccatc aaaaaagtc ctccaagac aagaggttt aagaaccgg gaacttcaa ataatgagg aaattagaa cattgtaat atagttaaa cttaaatat ttcttttac tccagtgga gataagaag gacgaagtg tacctgaat ttggtgttg gaatacatc ccagaaacg gtatacaga gtagcgaga cattacagc aaatcaaag caaacaatc ccgataatt tttatcaag ttgtatatg taccagtta ttccgatcg ttggcgtac atacactcg ttgggtatc tgtcatcgg gacattaaa ccgcaaaat cttctgctg gatccaggc actggcata ttgaagttg tgcgacttc ggcagcgct aaacacctc gtcaaaggc gaaccgaac gtctcgtac atttgcagt cgttattac agagcaccg gaactgata ttcggcgct atcgactac actacgaag atcgacgtg tggagtgcg ggctgtgtc ctggccgaa ttactgctg ggccagccg atattcccc ggagattcc ggtgtagac caattagta gaaattatc aaagtttta ggcacgcca actaaagaa cagatcaag gagatgaat ccgaattat acagagttc aagtttccg cagatcaag tcgcatcct tggcaacag gtattccga gcacggact cccccagaa gcgatcgag ttagtggcc agacttttg gagtacact ccttcatca cggatcagt ccattgcag gcttgtgct cacgcattc ttcaacgag ctccgggac cccaacacc cgcttgccc acaaataaa gaactgccc cctctattc aacttcacg gaacaagag ttgagtatt caaccttca ttgaattcc attctgata ccgcgaggg gtgcaggag gccgccaac agtccgcaa gacggggcc gcgtcgtcg ggggcgacg gacgccgcc gacacgacg gcagccccc gtgcaagcg accgccgcc ggaatacct taa
- >TC032822 RA
cgtttgagg tgcgtgaga aggacaggt cgtaagaag ggtgtcagc aagaaatgt gataggtct gcgaataat cgacttagg agaatttgc aatgagcgg acgccccag aaccacgtc cttcgccga gggcaacaa gcagccgct gaatccccc cctgggagg catgaagat tagcagtaa ggacggtag caaagttac tacggtggt agcaacacc tggacaagg tcccgacag accgcaaga agtctcata cactgacac caaagttat cggcaatgg cagcttcgg agtcgtcta tcaggccaa gctgtgcga aaccaacga gttcgtcgc catcaaaaa agtcctcca agacaagag gtttaagaa ccgggaact tcaaataat gaggaaatt agaacattg taatatagt taaacttaa atatttctt ttactccag tggagataa gaaggacga agtgtacct gaatttggt gttggaata catcccaga aacggtata cagagtagc gagacatta cagcaaatc aaagcaaac aatcccgat aatttttat caagttgta tatgtacca gttattccg atcgttggc gtacataca ctcgttggg tatctgtca tcgggacat taaaccgca aaatcttct gctggatcc aggcactgg catattgaa gttgtgcga cttcggcag cgctaaaca cctcgtcaa aggcgaacc gaacgtctc gtacatttg cagtcgtta ttacagagc accggaact gatattcgg cgctatcga ctacactac gaagatcga cgtgtggag tgcgggctg tgtcctggc cgaattact gctgggcca gccgatatt ccccggaga ttccggtgt agaccaatt agtagaaat tatcaaagt tttaggcac gccaactaa agaacagat caaggagat gaatccgaa ttatacaga gttcaagtt tccgcagat caagtcgca tccttggca acaggtatt ccgagcacg gactccccc agaagcgat cgagttagt ggccagact tttggagta cactccttc atcacggat cagtccatt gcaggcttg tgctcacgc attcttcaa cgagctccg ggaccccaa cacccgctt gcccacaaa taaagaact gccccctct attcaactt cacggaaca agagttgag tattcaacc ttcattgaa ttccattct gataccgcg aggggtgca ggaggccgc caacagtcc gcaagacgg ggccgcgtc gtcgggggc gacggacgc cgccgacac gacggcagc ccccgtgca agcgaccgc cgccggaat accttaagt agttcaatc ctccaaggg aagggcccc tttcgctcg tcccatttc tccctttat ctagtaagc gatctgtgt gagattgtt gattgggac agctgtact atatttaat atacgtgcg tttgtaca
- >TC032822 RB
cgtttgagg tgcgtgaga aggacaggt cgtaagaag ggtgtcagc aagaaatgt gataggtct gcgaataat cgacttagg agaatttgc aatgagcgg acgccccag aaccacgtc cttcgccga gggcaacaa gcagccgct gaatccccc cctgggagg catgaagat tagcagtct actaactga cgcaaaaac gtttcggga cggtaacga ttggggctc cgggggcgg cggcgcccc cgagacggc cgccaccgc gcggggcgc gcccattgc gcgcccagc gggccttcc catccgcgg aaattcctc caaattaac gacgatttt ccgaatttt tgccgacga ctctaaagt cgcaagtga tgcgaatta tgtggttaa aaaacagtg cgagtgtaa gaaattgca aagtgtttt gcccgaaaa gaacaaaaa cgcgagacc taagtacgg caggtcagt ttctgtgtg cgatttatc ttcggacaa gcacaagtc gaccaagaa aagtgcgac tttggggtc ggtgccggc gatcgatgg actggggaa aagccaaaa aacgacggg ctactccag gttgttgta ttgctggag gaagttgaa gaggtcgct gtcgagctc gggaagtaa agaaaaagg ccgtgaagt gttcgacga cgatcaata cgtcattgc ttcgaccaa actcaagtc aggtaagga cggtagcaa agttactac ggtggtagc aacacctgg acaaggtcc cgacagacc gcaagaagt ctcatacac tgacaccaa agttatcgg caatggcag cttcggagt cgtctatca ggccaagct gtgcgaaac caacgagtt cgtcgccat caaaaaagt cctccaaga caagaggtt taagaaccg ggaacttca aataatgag gaaattaga acattgtaa tatagttaa acttaaata tttctttta ctccagtgg agataagaa ggacgaagt gtacctgaa tttggtgtt ggaatacat cccagaaac ggtatacag agtagcgag acattacag caaatcaaa gcaaacaat cccgataat ttttatcaa gttgtatat gtaccagtt attccgatc gttggcgta catacactc gttgggtat ctgtcatcg ggacattaa accgcaaaa tcttctgct ggatccagg cactggcat attgaagtt gtgcgactt cggcagcgc taaacacct cgtcaaagg cgaaccgaa cgtctcgta catttgcag tcgttatta cagagcacc ggaactgat attcggcgc tatcgacta cactacgaa gatcgacgt gtggagtgc gggctgtgt cctggccga attactgct gggccagcc gatattccc cggagattc cggtgtaga ccaattagt agaaattat caaagtttt aggcacgcc aactaaaga acagatcaa ggagatgaa tccgaatta tacagagtt caagtttcc gcagatcaa gtcgcatcc ttggcaaca ggtattccg agcacggac tcccccaga agcgatcga gttagtggc cagactttt ggagtacac tccttcatc acggatcag tccattgca ggcttgtgc tcacgcatt cttcaacga gctccggga ccccaacac ccgcttgcc cacaaataa agaactgcc ccctctatt caacttcac ggaacaaga gttgagtat tcaaccttc attgaattc cattctgat accgcgagg ggtgcagga ggccgccaa cagtccgca agacggggc cgcgtcgtc gggggcgac ggacgccgc cgacacgac ggcagcccc cgtgcaagc gaccgccgc cggaatacc ttaagtagt tcaatcctc caagggaag ggccccttt cgctcgtcc catttctcc ctttatcta gtaagcgat ctgtgtgag attgttgat tgggacagc tgtactata tttaatata cgtgcgttt gtaca
