Status
Please wait ...
Gene information
General information
TC032822
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008141,TC008140
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5625376..5646464
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032822
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06250

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032822 RA
      MSGRPRTTS FAEGNKQPL NPPLGGMKI SSKDGSKVT 
      TVVATPGQG PDRPQEVSY TDTKVIGNG SFGVVYQAK 
      LCETNEFVA IKKVLQDKR FKNRELQIM RKLEHCNIV 
      KLKYFFYSS GDKKDEVYL NLVLEYIPE TVYRVARHY 
      SKSKQTIPI IFIKLYMYQ LFRSLAYIH SLGICHRDI 
      KPQNLLLDP GTGILKLCD FGSAKHLVK GEPNVSYIC 
      SRYYRAPEL IFGAIDYTT KIDVWSAGC VLAELLLGQ 
      PIFPGDSGV DQLVEIIKV LGTPTKEQI KEMNPNYTE 
      FKFPQIKSH PWQQVFRAR TPPEAIELV ARLLEYTPS 
      SRISPLQAC AHAFFNELR DPNTRLPTN KELPPLFNF 
      TEQELSIQP SLNSILIPR GVQEAANSP QDGAASSGA 
      TDAADTTAA PVQATAAGI P
    • >TC032822 RB
      MSGRPRTTS FAEGNKQPL NPPLGGMKI SSLLTDAKT 
      FRDGNDWGS GGGGAPETA ATARGAPIA RPAGLPIRG 
      NSSKLTTIF RIFADDSKV ASDANYVVK KQCECKKLQ 
      SVLPEKNKN ARPKYGRSV SVCDLSSDK HKSTKKSAT 
      LGSVPAIDG LGKSQKTTG YSRLLYCWR KLKRSLSSS 
      GSKEKGREV FDDDQYVIA STKLKSGKD GSKVTTVVA 
      TPGQGPDRP QEVSYTDTK VIGNGSFGV VYQAKLCET 
      NEFVAIKKV LQDKRFKNR ELQIMRKLE HCNIVKLKY 
      FFYSSGDKK DEVYLNLVL EYIPETVYR VARHYSKSK 
      QTIPIIFIK LYMYQLFRS LAYIHSLGI CHRDIKPQN 
      LLLDPGTGI LKLCDFGSA KHLVKGEPN VSYICSRYY 
      RAPELIFGA IDYTTKIDV WSAGCVLAE LLLGQPIFP 
      GDSGVDQLV EIIKVLGTP TKEQIKEMN PNYTEFKFP 
      QIKSHPWQQ VFRARTPPE AIELVARLL EYTPSSRIS 
      PLQACAHAF FNELRDPNT RLPTNKELP PLFNFTEQE 
      LSIQPSLNS ILIPRGVQE AANSPQDGA ASSGATDAA 
      DTTAAPVQA TAAGIP
    CDS sequence
    • >TC032822 RA
      atgagcgga cgccccaga accacgtcc ttcgccgag 
      ggcaacaag cagccgctg aatcccccc ctgggaggc 
      atgaagatt agcagtaag gacggtagc aaagttact 
      acggtggta gcaacacct ggacaaggt cccgacaga 
      ccgcaagaa gtctcatac actgacacc aaagttatc 
      ggcaatggc agcttcgga gtcgtctat caggccaag 
      ctgtgcgaa accaacgag ttcgtcgcc atcaaaaaa 
      gtcctccaa gacaagagg tttaagaac cgggaactt 
      caaataatg aggaaatta gaacattgt aatatagtt 
      aaacttaaa tatttcttt tactccagt ggagataag 
      aaggacgaa gtgtacctg aatttggtg ttggaatac 
      atcccagaa acggtatac agagtagcg agacattac 
      agcaaatca aagcaaaca atcccgata atttttatc 
      aagttgtat atgtaccag ttattccga tcgttggcg 
      tacatacac tcgttgggt atctgtcat cgggacatt 
      aaaccgcaa aatcttctg ctggatcca ggcactggc 
      atattgaag ttgtgcgac ttcggcagc gctaaacac 
      ctcgtcaaa ggcgaaccg aacgtctcg tacatttgc 
      agtcgttat tacagagca ccggaactg atattcggc 
      gctatcgac tacactacg aagatcgac gtgtggagt 
      gcgggctgt gtcctggcc gaattactg ctgggccag 
      ccgatattc cccggagat tccggtgta gaccaatta 
      gtagaaatt atcaaagtt ttaggcacg ccaactaaa 
      gaacagatc aaggagatg aatccgaat tatacagag 
      ttcaagttt ccgcagatc aagtcgcat ccttggcaa 
      caggtattc cgagcacgg actccccca gaagcgatc 
      gagttagtg gccagactt ttggagtac actccttca 
      tcacggatc agtccattg caggcttgt gctcacgca 
      ttcttcaac gagctccgg gaccccaac acccgcttg 
      cccacaaat aaagaactg ccccctcta ttcaacttc 
      acggaacaa gagttgagt attcaacct tcattgaat 
      tccattctg ataccgcga ggggtgcag gaggccgcc 
      aacagtccg caagacggg gccgcgtcg tcgggggcg 
      acggacgcc gccgacacg acggcagcc cccgtgcaa 
      gcgaccgcc gccggaata ccttaa
    • >TC032822 RB
      atgagcgga cgccccaga accacgtcc ttcgccgag 
      ggcaacaag cagccgctg aatcccccc ctgggaggc 
      atgaagatt agcagtcta ctaactgac gcaaaaacg 
      tttcgggac ggtaacgat tggggctcc gggggcggc 
      ggcgccccc gagacggcc gccaccgcg cggggcgcg 
      cccattgcg cgcccagcg ggccttccc atccgcgga 
      aattcctcc aaattaacg acgattttc cgaattttt 
      gccgacgac tctaaagtc gcaagtgat gcgaattat 
      gtggttaaa aaacagtgc gagtgtaag aaattgcaa 
      agtgttttg cccgaaaag aacaaaaac gcgagacct 
      aagtacggc aggtcagtt tctgtgtgc gatttatct 
      tcggacaag cacaagtcg accaagaaa agtgcgact 
      ttggggtcg gtgccggcg atcgatgga ctggggaaa 
      agccaaaaa acgacgggc tactccagg ttgttgtat 
      tgctggagg aagttgaag aggtcgctg tcgagctcg 
      ggaagtaaa gaaaaaggc cgtgaagtg ttcgacgac 
      gatcaatac gtcattgct tcgaccaaa ctcaagtca 
      ggtaaggac ggtagcaaa gttactacg gtggtagca 
      acacctgga caaggtccc gacagaccg caagaagtc 
      tcatacact gacaccaaa gttatcggc aatggcagc 
      ttcggagtc gtctatcag gccaagctg tgcgaaacc 
      aacgagttc gtcgccatc aaaaaagtc ctccaagac 
      aagaggttt aagaaccgg gaacttcaa ataatgagg 
      aaattagaa cattgtaat atagttaaa cttaaatat 
      ttcttttac tccagtgga gataagaag gacgaagtg 
      tacctgaat ttggtgttg gaatacatc ccagaaacg 
      gtatacaga gtagcgaga cattacagc aaatcaaag 
      caaacaatc ccgataatt tttatcaag ttgtatatg 
      taccagtta ttccgatcg ttggcgtac atacactcg 
      ttgggtatc tgtcatcgg gacattaaa ccgcaaaat 
      cttctgctg gatccaggc actggcata ttgaagttg 
      tgcgacttc ggcagcgct aaacacctc gtcaaaggc 
      gaaccgaac gtctcgtac atttgcagt cgttattac 
      agagcaccg gaactgata ttcggcgct atcgactac 
      actacgaag atcgacgtg tggagtgcg ggctgtgtc 
      ctggccgaa ttactgctg ggccagccg atattcccc 
      ggagattcc ggtgtagac caattagta gaaattatc 
      aaagtttta ggcacgcca actaaagaa cagatcaag 
      gagatgaat ccgaattat acagagttc aagtttccg 
      cagatcaag tcgcatcct tggcaacag gtattccga 
      gcacggact cccccagaa gcgatcgag ttagtggcc 
      agacttttg gagtacact ccttcatca cggatcagt 
      ccattgcag gcttgtgct cacgcattc ttcaacgag 
      ctccgggac cccaacacc cgcttgccc acaaataaa 
      gaactgccc cctctattc aacttcacg gaacaagag 
      ttgagtatt caaccttca ttgaattcc attctgata 
      ccgcgaggg gtgcaggag gccgccaac agtccgcaa 
      gacggggcc gcgtcgtcg ggggcgacg gacgccgcc 
      gacacgacg gcagccccc gtgcaagcg accgccgcc 
      ggaatacct taa
    mRNA sequence
    • >TC032822 RA
      cgtttgagg tgcgtgaga aggacaggt cgtaagaag 
      ggtgtcagc aagaaatgt gataggtct gcgaataat 
      cgacttagg agaatttgc aatgagcgg acgccccag 
      aaccacgtc cttcgccga gggcaacaa gcagccgct 
      gaatccccc cctgggagg catgaagat tagcagtaa 
      ggacggtag caaagttac tacggtggt agcaacacc 
      tggacaagg tcccgacag accgcaaga agtctcata 
      cactgacac caaagttat cggcaatgg cagcttcgg 
      agtcgtcta tcaggccaa gctgtgcga aaccaacga 
      gttcgtcgc catcaaaaa agtcctcca agacaagag 
      gtttaagaa ccgggaact tcaaataat gaggaaatt 
      agaacattg taatatagt taaacttaa atatttctt 
      ttactccag tggagataa gaaggacga agtgtacct 
      gaatttggt gttggaata catcccaga aacggtata 
      cagagtagc gagacatta cagcaaatc aaagcaaac 
      aatcccgat aatttttat caagttgta tatgtacca 
      gttattccg atcgttggc gtacataca ctcgttggg 
      tatctgtca tcgggacat taaaccgca aaatcttct 
      gctggatcc aggcactgg catattgaa gttgtgcga 
      cttcggcag cgctaaaca cctcgtcaa aggcgaacc 
      gaacgtctc gtacatttg cagtcgtta ttacagagc 
      accggaact gatattcgg cgctatcga ctacactac 
      gaagatcga cgtgtggag tgcgggctg tgtcctggc 
      cgaattact gctgggcca gccgatatt ccccggaga 
      ttccggtgt agaccaatt agtagaaat tatcaaagt 
      tttaggcac gccaactaa agaacagat caaggagat 
      gaatccgaa ttatacaga gttcaagtt tccgcagat 
      caagtcgca tccttggca acaggtatt ccgagcacg 
      gactccccc agaagcgat cgagttagt ggccagact 
      tttggagta cactccttc atcacggat cagtccatt 
      gcaggcttg tgctcacgc attcttcaa cgagctccg 
      ggaccccaa cacccgctt gcccacaaa taaagaact 
      gccccctct attcaactt cacggaaca agagttgag 
      tattcaacc ttcattgaa ttccattct gataccgcg 
      aggggtgca ggaggccgc caacagtcc gcaagacgg 
      ggccgcgtc gtcgggggc gacggacgc cgccgacac 
      gacggcagc ccccgtgca agcgaccgc cgccggaat 
      accttaagt agttcaatc ctccaaggg aagggcccc 
      tttcgctcg tcccatttc tccctttat ctagtaagc 
      gatctgtgt gagattgtt gattgggac agctgtact 
      atatttaat atacgtgcg tttgtaca
    • >TC032822 RB
      cgtttgagg tgcgtgaga aggacaggt cgtaagaag 
      ggtgtcagc aagaaatgt gataggtct gcgaataat 
      cgacttagg agaatttgc aatgagcgg acgccccag 
      aaccacgtc cttcgccga gggcaacaa gcagccgct 
      gaatccccc cctgggagg catgaagat tagcagtct 
      actaactga cgcaaaaac gtttcggga cggtaacga 
      ttggggctc cgggggcgg cggcgcccc cgagacggc 
      cgccaccgc gcggggcgc gcccattgc gcgcccagc 
      gggccttcc catccgcgg aaattcctc caaattaac 
      gacgatttt ccgaatttt tgccgacga ctctaaagt 
      cgcaagtga tgcgaatta tgtggttaa aaaacagtg 
      cgagtgtaa gaaattgca aagtgtttt gcccgaaaa 
      gaacaaaaa cgcgagacc taagtacgg caggtcagt 
      ttctgtgtg cgatttatc ttcggacaa gcacaagtc 
      gaccaagaa aagtgcgac tttggggtc ggtgccggc 
      gatcgatgg actggggaa aagccaaaa aacgacggg 
      ctactccag gttgttgta ttgctggag gaagttgaa 
      gaggtcgct gtcgagctc gggaagtaa agaaaaagg 
      ccgtgaagt gttcgacga cgatcaata cgtcattgc 
      ttcgaccaa actcaagtc aggtaagga cggtagcaa 
      agttactac ggtggtagc aacacctgg acaaggtcc 
      cgacagacc gcaagaagt ctcatacac tgacaccaa 
      agttatcgg caatggcag cttcggagt cgtctatca 
      ggccaagct gtgcgaaac caacgagtt cgtcgccat 
      caaaaaagt cctccaaga caagaggtt taagaaccg 
      ggaacttca aataatgag gaaattaga acattgtaa 
      tatagttaa acttaaata tttctttta ctccagtgg 
      agataagaa ggacgaagt gtacctgaa tttggtgtt 
      ggaatacat cccagaaac ggtatacag agtagcgag 
      acattacag caaatcaaa gcaaacaat cccgataat 
      ttttatcaa gttgtatat gtaccagtt attccgatc 
      gttggcgta catacactc gttgggtat ctgtcatcg 
      ggacattaa accgcaaaa tcttctgct ggatccagg 
      cactggcat attgaagtt gtgcgactt cggcagcgc 
      taaacacct cgtcaaagg cgaaccgaa cgtctcgta 
      catttgcag tcgttatta cagagcacc ggaactgat 
      attcggcgc tatcgacta cactacgaa gatcgacgt 
      gtggagtgc gggctgtgt cctggccga attactgct 
      gggccagcc gatattccc cggagattc cggtgtaga 
      ccaattagt agaaattat caaagtttt aggcacgcc 
      aactaaaga acagatcaa ggagatgaa tccgaatta 
      tacagagtt caagtttcc gcagatcaa gtcgcatcc 
      ttggcaaca ggtattccg agcacggac tcccccaga 
      agcgatcga gttagtggc cagactttt ggagtacac 
      tccttcatc acggatcag tccattgca ggcttgtgc 
      tcacgcatt cttcaacga gctccggga ccccaacac 
      ccgcttgcc cacaaataa agaactgcc ccctctatt 
      caacttcac ggaacaaga gttgagtat tcaaccttc 
      attgaattc cattctgat accgcgagg ggtgcagga 
      ggccgccaa cagtccgca agacggggc cgcgtcgtc 
      gggggcgac ggacgccgc cgacacgac ggcagcccc 
      cgtgcaagc gaccgccgc cggaatacc ttaagtagt 
      tcaatcctc caagggaag ggccccttt cgctcgtcc 
      catttctcc ctttatcta gtaagcgat ctgtgtgag 
      attgttgat tgggacagc tgtactata tttaatata 
      cgtgcgttt gtaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY