General information | |||
TC008111 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5069083..5073517 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008111 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06249 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 10 (16 dpi)
labial palp sometimesnot fused - Number of animals showing the phenotype: 6 (16 dpi)
labrum shortened - Number of animals showing the phenotype: 10 (16 dpi)
antenna shortened - Number of animals showing the phenotype: 10 (16 dpi)
head orientation irregular - Number of animals showing the phenotype: 10 (16 dpi)
elytra not present - Number of animals showing the phenotype: 10 (16 dpi)
legs shortened - Number of animals showing the phenotype: 10 (16 dpi)
dorsal thoracic muscles irregular - Number of animals showing the phenotype: 10 (16 dpi)
- >TC008111 RA
MNELAVPPA PLWYRSSVL ACAPDNTLV YGAKSDIVI VREKGPEEP AEVKLIHSA HSKLLTSVS LNRRWKEPH SYVVSLGED SVMKVWDLD TFEKKTSNR DHVEHKNPV IGAVFAGDD RVVSASSTG FVHVWNINT NESKPLVDL FKSKVTLTC LSSCPHAGW LMAFGLNNG VVIVADLRR NGQVLYKLR GHYQAVVCL SWCPAPVNI FPRHGQNTV KNKSSESEH NEGSSSDKT VETKEKVNP WVNLVHADD GEASSGKET LSDTISDFL KDCHLLKSK ILGLEPELV KSAEVKNSA ADNEAESEV ECEREFVPE VDCGRPLPK LYDPSGDFL DDSVLDSEI IEDKDEGNE KDIEHTGSE VKTDQTDET REKFDSPEK SECDSVLES ETEKHDNTP EESPKIATP EESPKVVEP IEEEPRAEY LLASCGKDK SVYIWRAGT DGRMQTFFQ AAKKGNNRR KNESTWMSL CWIAPDLLL TSSTKDELL AWPLPKPKT PKQYRVVHL EHSSLIFNI VAPPKYTTA FNWREKHEL SAWTWSQDG NLVCFDLDA EKTRMCFSC ITAFITCCD TSPLDPNRL AIGLKDGGI KIWDLSLPH TKTITMQKY HPKYTHKVT TIAWHPTRE MFLGFGTEE GRVGSIDLI SKKTTLFSH YFHSQVYKV EWGPLNADL HNLGLYAVA EGKIIIYDV SNPDQGPSF LDLPNNLYV YKFAWKPDF RLLLLNGKD GSLLVLTID LVAITTLYP NEKGLNGIV WHPDSFMDK TDVSPRCNW FAATTVKGI LVYDCGSIR ESDNFAENI VATFEGHVK PPLALAWCL FDGNRIAST ASDGLVHVW DVSTKSAVA TFVDFCLSH NPAVVWSPI DPDLIITGD RMIQVWRVS KNPPKLDEE FLAERKRLV AKMAKSDDK VDKSRFLIE VPKAKTPAK NYIVPSFYV SNDENLEMQ VGNMKKLLD QVENKNTGD GKLGPLELF GSKEQVSQL LDTNYNQQK KEGKFKSCE LISLWKGDV GDYIQTAID ENRVNPTTI NLAAMVSPK LWQDACLSY AKKLSEDAA SDPFETAMY FLACHKIEE AINSLCTSL MFKEALVLA KQRLPENSV FITEVTQKW AEHCVTTGN FENAAFCYI SIKEYENAA KVLARRNHK EFLDFAAEL AEKAGNKGL HDSIKYKCS ELENTPQNG AGDEMPSRI DAVLEKMTE NQDAVSESG SEITNGSHP ESAHNEN
- >TC008111 RA
atgaacgag ttagctgtt cctcctgcg cccctttgg tacaggagt tcagtgttg gcgtgtgcc cctgacaat accttggtg tatggcgca aaaagcgat attgttatc gttagggaa aaaggcccc gaagaaccg gccgaagtg aaattgatt cattcggct cactctaaa cttctcact tccgttagt ttgaacagg agatggaag gagccccat tcgtacgtg gtgtccctg ggggaagac agcgtgatg aaggtgtgg gacttggat accttcgag aagaaaacg tcgaatagg gatcatgtg gaacacaaa aaccccgta attggggcc gtttttgcg ggtgatgat cgcgtcgta agtgcgtca tcgaccggt tttgtacac gtctggaat atcaacacg aatgagtcc aagcctttg gtagattta ttcaaaagt aaagtcact ctgacttgt ctttcgtcg tgcccccat gccggctgg ctcatggcc tttggacta aataacggt gtggtgatc gtggccgat ttgcgtcgc aatgggcaa gttttgtat aaattgagg gggcattat caggcggtt gtttgcttg tcttggtgt ccagcaccc gtaaatatc tttccgaga catggccag aatacggtt aaaaataaa tcaagcgaa agtgagcac aatgagggt tctagttca gataagaca gttgagacc aaagaaaag gtcaatcct tgggtaaat ctagttcat gcagatgat ggcgaagcc tcatctggt aaagaaacc ctctcggac acaatctcc gattttttg aaagattgt catcttttg aaaagtaag atattaggg cttgaacca gaacttgta aaatcagcg gaagtaaaa aactcagcc gctgataac gaggcagaa agcgaagtt gaatgtgaa cgtgagttt gtgcctgaa gtggactgt ggtaggcct ctgccgaaa ctgtacgac ccaagtggc gactttttg gacgattct gtgctagat tccgaaatt atcgaagat aaagatgaa ggaaatgag aaggatatt gagcacacg ggctcagaa gtgaagact gatcaaacg gacgaaacc cgcgagaag tttgactcg ccggaaaaa tccgaatgt gatagcgtt ctggaatca gaaactgaa aaacatgat aatactcca gaggagtcg ccaaaaatt gctactcca gaggaatct ccaaaggtt gtagaaccg attgaggaa gaaccaaga gctgagtat ttgctcgca tcgtgtgga aaagacaaa agtgtttac atctggcgg gcggggact gacggtaga atgcagacg ttcttccaa gccgctaaa aagggcaac aatcgcagg aagaacgaa agcacttgg atgtccctg tgttggatt gccccagat ttattactg acctcgtca acaaaggac gaacttcta gcctggcct ttgcccaaa cccaaaaca cccaagcaa taccgagtt gtccacctt gaacactca tccctcatt ttcaacatc gtggcccct cccaagtac acgacggcg ttcaattgg cgcgaaaaa cacgaactt agtgcgtgg acttggagc caagacgga aacttggtt tgttttgac ttggacgcg gaaaaaaca cggatgtgt ttttcgtgc ataactgcg ttcatcact tgttgcgat acttcacct ttggatccc aatcgactt gcgatcggt ttgaaagac ggtggtatc aaaatctgg gatttgagt ctcccacat actaaaact atcacaatg cagaaatac catcccaaa tatacgcac aaagttacc acgattgcc tggcaccct accagggaa atgtttcta gggttcggg actgaggaa ggacgcgtc ggctcgatt gacctcatt tcgaagaaa acaacgctt ttttctcac tactttcac agccaagtg tataaagtc gagtggggg cctttgaat gcggactta cacaactta gggctttat gcggtggct gagggcaag attatcatt tatgacgta tcaaacccc gaccaaggg ccgtctttc ttggacctt cccaacaac ttgtacgtc tataaattc gcctggaag ccggacttt cgtttgctt ttgttgaac ggaaaagac ggttctttg ttggtgcta acgatcgat ttagtggcc attactacg ctttatccg aacgaaaaa ggccttaac ggaatcgtg tggcatcct gattctttc atggacaaa accgacgtt tcccctcgt tgtaactgg tttgcagcg accacagtt aaagggatc ttggtctac gattgcggg tcaatccga gaaagtgac aattttgcc gaaaacatt gtcgctacg tttgaggga catgtgaag ccgcctttg gccttagcg tggtgtctt tttgatggt aatagaatc gcgtcgact gcttcggat ggactggtc catgtgtgg gacgtgtca acgaaatca gcagttgcg acttttgtt gacttctgc ctgagtcat aatccggca gttgtttgg agtccgatc gatccagat ttgattatt acaggtgat aggatgatt caggtttgg agagtttcc aaaaatccg ccaaaattg gacgaagag tttctagct gagaggaaa cgtcttgtg gctaaaatg gccaaaagc gatgacaaa gttgacaaa agtcgattt ttaattgag gtgccgaaa gccaaaact ccagctaaa aactacata gtgcctagt ttttacgta tcaaatgat gagaacctg gaaatgcaa gtgggcaat atgaaaaag ttgttagac caagttgaa aataaaaat acaggtgat ggcaagtta gggcccctt gagttattt gggtccaag gaacaagtg tcgcaactt ttagacaca aattataat caacagaaa aaagagggt aaatttaaa tcttgtgag ttgatttcg ctgtggaaa ggggacgta ggtgattat attcaaact gccattgat gagaatcgc gtcaacccc acgactatt aatttggcc gcaatggtt tcgccaaaa ttatggcaa gatgcgtgt ctgtcatac gcgaaaaaa ttgtcagag gatgccgcg tctgatccg ttcgagaca gcaatgtac tttttagcg tgccataaa atcgaagaa gcgataaat tcactgtgc accagtctc atgtttaag gaagcttta gttttggcc aaacagagg cttccggaa aatagcgtg tttatcacg gaagtgacg caaaaatgg gccgaacat tgtgtaacc accggcaat ttcgaaaat gctgctttt tgttatatt tcgattaag gaatatgaa aatgccgcc aaggtttta gcgcgaagg aatcataag gaatttctc gattttgca gccgaattg gccgaaaaa gcgggaaat aaaggactg catgactcg attaaatat aagtgtagt gaactggaa aatactcca caaaatggt gcaggtgac gaaatgccg tctcgaatt gatgccgtt ttggagaaa atgactgag aatcaagat gcagtttca gagagtggc tctgaaatt accaatgga tctcacccg gaatcagcc cataatgaa aattag
- >TC008111 RA
gtcttgttc agtgcacaa actcgccca accacatgt gaaaataac gtgtaaatg cgttttaaa agttacttg ttcagttgt tgaataatt tggtgagat gaacgagtt agctgttcc tcctgcgcc cctttggta caggagttc agtgttggc gtgtgcccc tgacaatac cttggtgta tggcgcaaa aagcgatat tgttatcgt tagggaaaa aggccccga agaaccggc cgaagtgaa attgattca ttcggctca ctctaaact tctcacttc cgttagttt gaacaggag atggaagga gccccattc gtacgtggt gtccctggg ggaagacag cgtgatgaa ggtgtggga cttggatac cttcgagaa gaaaacgtc gaataggga tcatgtgga acacaaaaa ccccgtaat tggggccgt ttttgcggg tgatgatcg cgtcgtaag tgcgtcatc gaccggttt tgtacacgt ctggaatat caacacgaa tgagtccaa gcctttggt agatttatt caaaagtaa agtcactct gacttgtct ttcgtcgtg cccccatgc cggctggct catggcctt tggactaaa taacggtgt ggtgatcgt ggccgattt gcgtcgcaa tgggcaagt tttgtataa attgagggg gcattatca ggcggttgt ttgcttgtc ttggtgtcc agcacccgt aaatatctt tccgagaca tggccagaa tacggttaa aaataaatc aagcgaaag tgagcacaa tgagggttc tagttcaga taagacagt tgagaccaa agaaaaggt caatccttg ggtaaatct agttcatgc agatgatgg cgaagcctc atctggtaa agaaaccct ctcggacac aatctccga ttttttgaa agattgtca tcttttgaa aagtaagat attagggct tgaaccaga acttgtaaa atcagcgga agtaaaaaa ctcagccgc tgataacga ggcagaaag cgaagttga atgtgaacg tgagtttgt gcctgaagt ggactgtgg taggcctct gccgaaact gtacgaccc aagtggcga ctttttgga cgattctgt gctagattc cgaaattat cgaagataa agatgaagg aaatgagaa ggatattga gcacacggg ctcagaagt gaagactga tcaaacgga cgaaacccg cgagaagtt tgactcgcc ggaaaaatc cgaatgtga tagcgttct ggaatcaga aactgaaaa acatgataa tactccaga ggagtcgcc aaaaattgc tactccaga ggaatctcc aaaggttgt agaaccgat tgaggaaga accaagagc tgagtattt gctcgcatc gtgtggaaa agacaaaag tgtttacat ctggcgggc ggggactga cggtagaat gcagacgtt cttccaagc cgctaaaaa gggcaacaa tcgcaggaa gaacgaaag cacttggat gtccctgtg ttggattgc cccagattt attactgac ctcgtcaac aaaggacga acttctagc ctggccttt gcccaaacc caaaacacc caagcaata ccgagttgt ccaccttga acactcatc cctcatttt caacatcgt ggcccctcc caagtacac gacggcgtt caattggcg cgaaaaaca cgaacttag tgcgtggac ttggagcca agacggaaa cttggtttg ttttgactt ggacgcgga aaaaacacg gatgtgttt ttcgtgcat aactgcgtt catcacttg ttgcgatac ttcaccttt ggatcccaa tcgacttgc gatcggttt gaaagacgg tggtatcaa aatctggga tttgagtct cccacatac taaaactat cacaatgca gaaatacca tcccaaata tacgcacaa agttaccac gattgcctg gcaccctac cagggaaat gtttctagg gttcgggac tgaggaagg acgcgtcgg ctcgattga cctcatttc gaagaaaac aacgctttt ttctcacta ctttcacag ccaagtgta taaagtcga gtgggggcc tttgaatgc ggacttaca caacttagg gctttatgc ggtggctga gggcaagat tatcattta tgacgtatc aaaccccga ccaagggcc gtctttctt ggaccttcc caacaactt gtacgtcta taaattcgc ctggaagcc ggactttcg tttgctttt gttgaacgg aaaagacgg ttctttgtt ggtgctaac gatcgattt agtggccat tactacgct ttatccgaa cgaaaaagg ccttaacgg aatcgtgtg gcatcctga ttctttcat ggacaaaac cgacgtttc ccctcgttg taactggtt tgcagcgac cacagttaa agggatctt ggtctacga ttgcgggtc aatccgaga aagtgacaa ttttgccga aaacattgt cgctacgtt tgagggaca tgtgaagcc gcctttggc cttagcgtg gtgtctttt tgatggtaa tagaatcgc gtcgactgc ttcggatgg actggtcca tgtgtggga cgtgtcaac gaaatcagc agttgcgac ttttgttga cttctgcct gagtcataa tccggcagt tgtttggag tccgatcga tccagattt gattattac aggtgatag gatgattca ggtttggag agtttccaa aaatccgcc aaaattgga cgaagagtt tctagctga gaggaaacg tcttgtggc taaaatggc caaaagcga tgacaaagt tgacaaaag tcgattttt aattgaggt gccgaaagc caaaactcc agctaaaaa ctacatagt gcctagttt ttacgtatc aaatgatga gaacctgga aatgcaagt gggcaatat gaaaaagtt gttagacca agttgaaaa taaaaatac aggtgatgg caagttagg gccccttga gttatttgg gtccaagga acaagtgtc gcaactttt agacacaaa ttataatca acagaaaaa agagggtaa atttaaatc ttgtgagtt gatttcgct gtggaaagg ggacgtagg tgattatat tcaaactgc cattgatga gaatcgcgt caaccccac gactattaa tttggccgc aatggtttc gccaaaatt atggcaaga tgcgtgtct gtcatacgc gaaaaaatt gtcagagga tgccgcgtc tgatccgtt cgagacagc aatgtactt tttagcgtg ccataaaat cgaagaagc gataaattc actgtgcac cagtctcat gtttaagga agctttagt tttggccaa acagaggct tccggaaaa tagcgtgtt tatcacgga agtgacgca aaaatgggc cgaacattg tgtaaccac cggcaattt cgaaaatgc tgctttttg ttatatttc gattaagga atatgaaaa tgccgccaa ggttttagc gcgaaggaa tcataagga atttctcga ttttgcagc cgaattggc cgaaaaagc gggaaataa aggactgca tgactcgat taaatataa gtgtagtga actggaaaa tactccaca aaatggtgc aggtgacga aatgccgtc tcgaattga tgccgtttt ggagaaaat gactgagaa tcaagatgc agtttcaga gagtggctc tgaaattac caatggatc tcacccgga atcagccca taatgaaaa ttaggcgcc actttaggc gtgatagaa gattgttta agttttaag actgaacca accgaatag tattttaat ttttatagt ttttgcttt gttttttat ttttgtttc gtttgtttt ctaagaata aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
