Status
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Gene information
General information
TC008111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5069083..5073517
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008111
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06249

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    labial palp sometimesnot fused - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    labrum shortened - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    antenna shortened - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    head orientation irregular - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    elytra not present - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    legs shortened - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    dorsal thoracic muscles irregular - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008111 RA
      MNELAVPPA PLWYRSSVL ACAPDNTLV YGAKSDIVI 
      VREKGPEEP AEVKLIHSA HSKLLTSVS LNRRWKEPH 
      SYVVSLGED SVMKVWDLD TFEKKTSNR DHVEHKNPV 
      IGAVFAGDD RVVSASSTG FVHVWNINT NESKPLVDL 
      FKSKVTLTC LSSCPHAGW LMAFGLNNG VVIVADLRR 
      NGQVLYKLR GHYQAVVCL SWCPAPVNI FPRHGQNTV 
      KNKSSESEH NEGSSSDKT VETKEKVNP WVNLVHADD 
      GEASSGKET LSDTISDFL KDCHLLKSK ILGLEPELV 
      KSAEVKNSA ADNEAESEV ECEREFVPE VDCGRPLPK 
      LYDPSGDFL DDSVLDSEI IEDKDEGNE KDIEHTGSE 
      VKTDQTDET REKFDSPEK SECDSVLES ETEKHDNTP 
      EESPKIATP EESPKVVEP IEEEPRAEY LLASCGKDK 
      SVYIWRAGT DGRMQTFFQ AAKKGNNRR KNESTWMSL 
      CWIAPDLLL TSSTKDELL AWPLPKPKT PKQYRVVHL 
      EHSSLIFNI VAPPKYTTA FNWREKHEL SAWTWSQDG 
      NLVCFDLDA EKTRMCFSC ITAFITCCD TSPLDPNRL 
      AIGLKDGGI KIWDLSLPH TKTITMQKY HPKYTHKVT 
      TIAWHPTRE MFLGFGTEE GRVGSIDLI SKKTTLFSH 
      YFHSQVYKV EWGPLNADL HNLGLYAVA EGKIIIYDV 
      SNPDQGPSF LDLPNNLYV YKFAWKPDF RLLLLNGKD 
      GSLLVLTID LVAITTLYP NEKGLNGIV WHPDSFMDK 
      TDVSPRCNW FAATTVKGI LVYDCGSIR ESDNFAENI 
      VATFEGHVK PPLALAWCL FDGNRIAST ASDGLVHVW 
      DVSTKSAVA TFVDFCLSH NPAVVWSPI DPDLIITGD 
      RMIQVWRVS KNPPKLDEE FLAERKRLV AKMAKSDDK 
      VDKSRFLIE VPKAKTPAK NYIVPSFYV SNDENLEMQ 
      VGNMKKLLD QVENKNTGD GKLGPLELF GSKEQVSQL 
      LDTNYNQQK KEGKFKSCE LISLWKGDV GDYIQTAID 
      ENRVNPTTI NLAAMVSPK LWQDACLSY AKKLSEDAA 
      SDPFETAMY FLACHKIEE AINSLCTSL MFKEALVLA 
      KQRLPENSV FITEVTQKW AEHCVTTGN FENAAFCYI 
      SIKEYENAA KVLARRNHK EFLDFAAEL AEKAGNKGL 
      HDSIKYKCS ELENTPQNG AGDEMPSRI DAVLEKMTE 
      NQDAVSESG SEITNGSHP ESAHNEN
    CDS sequence
    • >TC008111 RA
      atgaacgag ttagctgtt cctcctgcg cccctttgg 
      tacaggagt tcagtgttg gcgtgtgcc cctgacaat 
      accttggtg tatggcgca aaaagcgat attgttatc 
      gttagggaa aaaggcccc gaagaaccg gccgaagtg 
      aaattgatt cattcggct cactctaaa cttctcact 
      tccgttagt ttgaacagg agatggaag gagccccat 
      tcgtacgtg gtgtccctg ggggaagac agcgtgatg 
      aaggtgtgg gacttggat accttcgag aagaaaacg 
      tcgaatagg gatcatgtg gaacacaaa aaccccgta 
      attggggcc gtttttgcg ggtgatgat cgcgtcgta 
      agtgcgtca tcgaccggt tttgtacac gtctggaat 
      atcaacacg aatgagtcc aagcctttg gtagattta 
      ttcaaaagt aaagtcact ctgacttgt ctttcgtcg 
      tgcccccat gccggctgg ctcatggcc tttggacta 
      aataacggt gtggtgatc gtggccgat ttgcgtcgc 
      aatgggcaa gttttgtat aaattgagg gggcattat 
      caggcggtt gtttgcttg tcttggtgt ccagcaccc 
      gtaaatatc tttccgaga catggccag aatacggtt 
      aaaaataaa tcaagcgaa agtgagcac aatgagggt 
      tctagttca gataagaca gttgagacc aaagaaaag 
      gtcaatcct tgggtaaat ctagttcat gcagatgat 
      ggcgaagcc tcatctggt aaagaaacc ctctcggac 
      acaatctcc gattttttg aaagattgt catcttttg 
      aaaagtaag atattaggg cttgaacca gaacttgta 
      aaatcagcg gaagtaaaa aactcagcc gctgataac 
      gaggcagaa agcgaagtt gaatgtgaa cgtgagttt 
      gtgcctgaa gtggactgt ggtaggcct ctgccgaaa 
      ctgtacgac ccaagtggc gactttttg gacgattct 
      gtgctagat tccgaaatt atcgaagat aaagatgaa 
      ggaaatgag aaggatatt gagcacacg ggctcagaa 
      gtgaagact gatcaaacg gacgaaacc cgcgagaag 
      tttgactcg ccggaaaaa tccgaatgt gatagcgtt 
      ctggaatca gaaactgaa aaacatgat aatactcca 
      gaggagtcg ccaaaaatt gctactcca gaggaatct 
      ccaaaggtt gtagaaccg attgaggaa gaaccaaga 
      gctgagtat ttgctcgca tcgtgtgga aaagacaaa 
      agtgtttac atctggcgg gcggggact gacggtaga 
      atgcagacg ttcttccaa gccgctaaa aagggcaac 
      aatcgcagg aagaacgaa agcacttgg atgtccctg 
      tgttggatt gccccagat ttattactg acctcgtca 
      acaaaggac gaacttcta gcctggcct ttgcccaaa 
      cccaaaaca cccaagcaa taccgagtt gtccacctt 
      gaacactca tccctcatt ttcaacatc gtggcccct 
      cccaagtac acgacggcg ttcaattgg cgcgaaaaa 
      cacgaactt agtgcgtgg acttggagc caagacgga 
      aacttggtt tgttttgac ttggacgcg gaaaaaaca 
      cggatgtgt ttttcgtgc ataactgcg ttcatcact 
      tgttgcgat acttcacct ttggatccc aatcgactt 
      gcgatcggt ttgaaagac ggtggtatc aaaatctgg 
      gatttgagt ctcccacat actaaaact atcacaatg 
      cagaaatac catcccaaa tatacgcac aaagttacc 
      acgattgcc tggcaccct accagggaa atgtttcta 
      gggttcggg actgaggaa ggacgcgtc ggctcgatt 
      gacctcatt tcgaagaaa acaacgctt ttttctcac 
      tactttcac agccaagtg tataaagtc gagtggggg 
      cctttgaat gcggactta cacaactta gggctttat 
      gcggtggct gagggcaag attatcatt tatgacgta 
      tcaaacccc gaccaaggg ccgtctttc ttggacctt 
      cccaacaac ttgtacgtc tataaattc gcctggaag 
      ccggacttt cgtttgctt ttgttgaac ggaaaagac 
      ggttctttg ttggtgcta acgatcgat ttagtggcc 
      attactacg ctttatccg aacgaaaaa ggccttaac 
      ggaatcgtg tggcatcct gattctttc atggacaaa 
      accgacgtt tcccctcgt tgtaactgg tttgcagcg 
      accacagtt aaagggatc ttggtctac gattgcggg 
      tcaatccga gaaagtgac aattttgcc gaaaacatt 
      gtcgctacg tttgaggga catgtgaag ccgcctttg 
      gccttagcg tggtgtctt tttgatggt aatagaatc 
      gcgtcgact gcttcggat ggactggtc catgtgtgg 
      gacgtgtca acgaaatca gcagttgcg acttttgtt 
      gacttctgc ctgagtcat aatccggca gttgtttgg 
      agtccgatc gatccagat ttgattatt acaggtgat 
      aggatgatt caggtttgg agagtttcc aaaaatccg 
      ccaaaattg gacgaagag tttctagct gagaggaaa 
      cgtcttgtg gctaaaatg gccaaaagc gatgacaaa 
      gttgacaaa agtcgattt ttaattgag gtgccgaaa 
      gccaaaact ccagctaaa aactacata gtgcctagt 
      ttttacgta tcaaatgat gagaacctg gaaatgcaa 
      gtgggcaat atgaaaaag ttgttagac caagttgaa 
      aataaaaat acaggtgat ggcaagtta gggcccctt 
      gagttattt gggtccaag gaacaagtg tcgcaactt 
      ttagacaca aattataat caacagaaa aaagagggt 
      aaatttaaa tcttgtgag ttgatttcg ctgtggaaa 
      ggggacgta ggtgattat attcaaact gccattgat 
      gagaatcgc gtcaacccc acgactatt aatttggcc 
      gcaatggtt tcgccaaaa ttatggcaa gatgcgtgt 
      ctgtcatac gcgaaaaaa ttgtcagag gatgccgcg 
      tctgatccg ttcgagaca gcaatgtac tttttagcg 
      tgccataaa atcgaagaa gcgataaat tcactgtgc 
      accagtctc atgtttaag gaagcttta gttttggcc 
      aaacagagg cttccggaa aatagcgtg tttatcacg 
      gaagtgacg caaaaatgg gccgaacat tgtgtaacc 
      accggcaat ttcgaaaat gctgctttt tgttatatt 
      tcgattaag gaatatgaa aatgccgcc aaggtttta 
      gcgcgaagg aatcataag gaatttctc gattttgca 
      gccgaattg gccgaaaaa gcgggaaat aaaggactg 
      catgactcg attaaatat aagtgtagt gaactggaa 
      aatactcca caaaatggt gcaggtgac gaaatgccg 
      tctcgaatt gatgccgtt ttggagaaa atgactgag 
      aatcaagat gcagtttca gagagtggc tctgaaatt 
      accaatgga tctcacccg gaatcagcc cataatgaa 
      aattag
    mRNA sequence
    • >TC008111 RA
      gtcttgttc agtgcacaa actcgccca accacatgt 
      gaaaataac gtgtaaatg cgttttaaa agttacttg 
      ttcagttgt tgaataatt tggtgagat gaacgagtt 
      agctgttcc tcctgcgcc cctttggta caggagttc 
      agtgttggc gtgtgcccc tgacaatac cttggtgta 
      tggcgcaaa aagcgatat tgttatcgt tagggaaaa 
      aggccccga agaaccggc cgaagtgaa attgattca 
      ttcggctca ctctaaact tctcacttc cgttagttt 
      gaacaggag atggaagga gccccattc gtacgtggt 
      gtccctggg ggaagacag cgtgatgaa ggtgtggga 
      cttggatac cttcgagaa gaaaacgtc gaataggga 
      tcatgtgga acacaaaaa ccccgtaat tggggccgt 
      ttttgcggg tgatgatcg cgtcgtaag tgcgtcatc 
      gaccggttt tgtacacgt ctggaatat caacacgaa 
      tgagtccaa gcctttggt agatttatt caaaagtaa 
      agtcactct gacttgtct ttcgtcgtg cccccatgc 
      cggctggct catggcctt tggactaaa taacggtgt 
      ggtgatcgt ggccgattt gcgtcgcaa tgggcaagt 
      tttgtataa attgagggg gcattatca ggcggttgt 
      ttgcttgtc ttggtgtcc agcacccgt aaatatctt 
      tccgagaca tggccagaa tacggttaa aaataaatc 
      aagcgaaag tgagcacaa tgagggttc tagttcaga 
      taagacagt tgagaccaa agaaaaggt caatccttg 
      ggtaaatct agttcatgc agatgatgg cgaagcctc 
      atctggtaa agaaaccct ctcggacac aatctccga 
      ttttttgaa agattgtca tcttttgaa aagtaagat 
      attagggct tgaaccaga acttgtaaa atcagcgga 
      agtaaaaaa ctcagccgc tgataacga ggcagaaag 
      cgaagttga atgtgaacg tgagtttgt gcctgaagt 
      ggactgtgg taggcctct gccgaaact gtacgaccc 
      aagtggcga ctttttgga cgattctgt gctagattc 
      cgaaattat cgaagataa agatgaagg aaatgagaa 
      ggatattga gcacacggg ctcagaagt gaagactga 
      tcaaacgga cgaaacccg cgagaagtt tgactcgcc 
      ggaaaaatc cgaatgtga tagcgttct ggaatcaga 
      aactgaaaa acatgataa tactccaga ggagtcgcc 
      aaaaattgc tactccaga ggaatctcc aaaggttgt 
      agaaccgat tgaggaaga accaagagc tgagtattt 
      gctcgcatc gtgtggaaa agacaaaag tgtttacat 
      ctggcgggc ggggactga cggtagaat gcagacgtt 
      cttccaagc cgctaaaaa gggcaacaa tcgcaggaa 
      gaacgaaag cacttggat gtccctgtg ttggattgc 
      cccagattt attactgac ctcgtcaac aaaggacga 
      acttctagc ctggccttt gcccaaacc caaaacacc 
      caagcaata ccgagttgt ccaccttga acactcatc 
      cctcatttt caacatcgt ggcccctcc caagtacac 
      gacggcgtt caattggcg cgaaaaaca cgaacttag 
      tgcgtggac ttggagcca agacggaaa cttggtttg 
      ttttgactt ggacgcgga aaaaacacg gatgtgttt 
      ttcgtgcat aactgcgtt catcacttg ttgcgatac 
      ttcaccttt ggatcccaa tcgacttgc gatcggttt 
      gaaagacgg tggtatcaa aatctggga tttgagtct 
      cccacatac taaaactat cacaatgca gaaatacca 
      tcccaaata tacgcacaa agttaccac gattgcctg 
      gcaccctac cagggaaat gtttctagg gttcgggac 
      tgaggaagg acgcgtcgg ctcgattga cctcatttc 
      gaagaaaac aacgctttt ttctcacta ctttcacag 
      ccaagtgta taaagtcga gtgggggcc tttgaatgc 
      ggacttaca caacttagg gctttatgc ggtggctga 
      gggcaagat tatcattta tgacgtatc aaaccccga 
      ccaagggcc gtctttctt ggaccttcc caacaactt 
      gtacgtcta taaattcgc ctggaagcc ggactttcg 
      tttgctttt gttgaacgg aaaagacgg ttctttgtt 
      ggtgctaac gatcgattt agtggccat tactacgct 
      ttatccgaa cgaaaaagg ccttaacgg aatcgtgtg 
      gcatcctga ttctttcat ggacaaaac cgacgtttc 
      ccctcgttg taactggtt tgcagcgac cacagttaa 
      agggatctt ggtctacga ttgcgggtc aatccgaga 
      aagtgacaa ttttgccga aaacattgt cgctacgtt 
      tgagggaca tgtgaagcc gcctttggc cttagcgtg 
      gtgtctttt tgatggtaa tagaatcgc gtcgactgc 
      ttcggatgg actggtcca tgtgtggga cgtgtcaac 
      gaaatcagc agttgcgac ttttgttga cttctgcct 
      gagtcataa tccggcagt tgtttggag tccgatcga 
      tccagattt gattattac aggtgatag gatgattca 
      ggtttggag agtttccaa aaatccgcc aaaattgga 
      cgaagagtt tctagctga gaggaaacg tcttgtggc 
      taaaatggc caaaagcga tgacaaagt tgacaaaag 
      tcgattttt aattgaggt gccgaaagc caaaactcc 
      agctaaaaa ctacatagt gcctagttt ttacgtatc 
      aaatgatga gaacctgga aatgcaagt gggcaatat 
      gaaaaagtt gttagacca agttgaaaa taaaaatac 
      aggtgatgg caagttagg gccccttga gttatttgg 
      gtccaagga acaagtgtc gcaactttt agacacaaa 
      ttataatca acagaaaaa agagggtaa atttaaatc 
      ttgtgagtt gatttcgct gtggaaagg ggacgtagg 
      tgattatat tcaaactgc cattgatga gaatcgcgt 
      caaccccac gactattaa tttggccgc aatggtttc 
      gccaaaatt atggcaaga tgcgtgtct gtcatacgc 
      gaaaaaatt gtcagagga tgccgcgtc tgatccgtt 
      cgagacagc aatgtactt tttagcgtg ccataaaat 
      cgaagaagc gataaattc actgtgcac cagtctcat 
      gtttaagga agctttagt tttggccaa acagaggct 
      tccggaaaa tagcgtgtt tatcacgga agtgacgca 
      aaaatgggc cgaacattg tgtaaccac cggcaattt 
      cgaaaatgc tgctttttg ttatatttc gattaagga 
      atatgaaaa tgccgccaa ggttttagc gcgaaggaa 
      tcataagga atttctcga ttttgcagc cgaattggc 
      cgaaaaagc gggaaataa aggactgca tgactcgat 
      taaatataa gtgtagtga actggaaaa tactccaca 
      aaatggtgc aggtgacga aatgccgtc tcgaattga 
      tgccgtttt ggagaaaat gactgagaa tcaagatgc 
      agtttcaga gagtggctc tgaaattac caatggatc 
      tcacccgga atcagccca taatgaaaa ttaggcgcc 
      actttaggc gtgatagaa gattgttta agttttaag 
      actgaacca accgaatag tattttaat ttttatagt 
      ttttgcttt gttttttat ttttgtttc gtttgtttt 
      ctaagaata aa
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