Status
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Gene information
General information
TC008111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5069083..5073517
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008111
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06249

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection

    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    labial palp sometimesnot fused - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    labrum shortened - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection

    antenna shortened - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection

    head orientation irregular - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    elytra not present - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    legs shortened - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    dorsal thoracic muscles irregular - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008111 RA
      MNELAVPPA PLWYRSSVL ACAPDNTLV YGAKSDIVI 
VREKGPEEP AEVKLIHSA HSKLLTSVS LNRRWKEPH 
SYVVSLGED SVMKVWDLD TFEKKTSNR DHVEHKNPV 
IGAVFAGDD RVVSASSTG FVHVWNINT NESKPLVDL 
FKSKVTLTC LSSCPHAGW LMAFGLNNG VVIVADLRR 
NGQVLYKLR GHYQAVVCL SWCPAPVNI FPRHGQNTV 
KNKSSESEH NEGSSSDKT VETKEKVNP WVNLVHADD 
GEASSGKET LSDTISDFL KDCHLLKSK ILGLEPELV 
KSAEVKNSA ADNEAESEV ECEREFVPE VDCGRPLPK 
LYDPSGDFL DDSVLDSEI IEDKDEGNE KDIEHTGSE 
VKTDQTDET REKFDSPEK SECDSVLES ETEKHDNTP 
EESPKIATP EESPKVVEP IEEEPRAEY LLASCGKDK 
SVYIWRAGT DGRMQTFFQ AAKKGNNRR KNESTWMSL 
CWIAPDLLL TSSTKDELL AWPLPKPKT PKQYRVVHL 
EHSSLIFNI VAPPKYTTA FNWREKHEL SAWTWSQDG 
NLVCFDLDA EKTRMCFSC ITAFITCCD TSPLDPNRL 
AIGLKDGGI KIWDLSLPH TKTITMQKY HPKYTHKVT 
TIAWHPTRE MFLGFGTEE GRVGSIDLI SKKTTLFSH 
YFHSQVYKV EWGPLNADL HNLGLYAVA EGKIIIYDV 
SNPDQGPSF LDLPNNLYV YKFAWKPDF RLLLLNGKD 
GSLLVLTID LVAITTLYP NEKGLNGIV WHPDSFMDK 
TDVSPRCNW FAATTVKGI LVYDCGSIR ESDNFAENI 
VATFEGHVK PPLALAWCL FDGNRIAST ASDGLVHVW 
DVSTKSAVA TFVDFCLSH NPAVVWSPI DPDLIITGD 
RMIQVWRVS KNPPKLDEE FLAERKRLV AKMAKSDDK 
VDKSRFLIE VPKAKTPAK NYIVPSFYV SNDENLEMQ 
VGNMKKLLD QVENKNTGD GKLGPLELF GSKEQVSQL 
LDTNYNQQK KEGKFKSCE LISLWKGDV GDYIQTAID 
ENRVNPTTI NLAAMVSPK LWQDACLSY AKKLSEDAA 
SDPFETAMY FLACHKIEE AINSLCTSL MFKEALVLA 
KQRLPENSV FITEVTQKW AEHCVTTGN FENAAFCYI 
SIKEYENAA KVLARRNHK EFLDFAAEL AEKAGNKGL 
HDSIKYKCS ELENTPQNG AGDEMPSRI DAVLEKMTE 
NQDAVSESG SEITNGSHP ESAHNEN
    CDS sequence
    • >TC008111 RA
      atgaacgag ttagctgtt cctcctgcg cccctttgg 
tacaggagt tcagtgttg gcgtgtgcc cctgacaat 
accttggtg tatggcgca aaaagcgat attgttatc 
gttagggaa aaaggcccc gaagaaccg gccgaagtg 
aaattgatt cattcggct cactctaaa cttctcact 
tccgttagt ttgaacagg agatggaag gagccccat 
tcgtacgtg gtgtccctg ggggaagac agcgtgatg 
aaggtgtgg gacttggat accttcgag aagaaaacg 
tcgaatagg gatcatgtg gaacacaaa aaccccgta 
attggggcc gtttttgcg ggtgatgat cgcgtcgta 
agtgcgtca tcgaccggt tttgtacac gtctggaat 
atcaacacg aatgagtcc aagcctttg gtagattta 
ttcaaaagt aaagtcact ctgacttgt ctttcgtcg 
tgcccccat gccggctgg ctcatggcc tttggacta 
aataacggt gtggtgatc gtggccgat ttgcgtcgc 
aatgggcaa gttttgtat aaattgagg gggcattat 
caggcggtt gtttgcttg tcttggtgt ccagcaccc 
gtaaatatc tttccgaga catggccag aatacggtt 
aaaaataaa tcaagcgaa agtgagcac aatgagggt 
tctagttca gataagaca gttgagacc aaagaaaag 
gtcaatcct tgggtaaat ctagttcat gcagatgat 
ggcgaagcc tcatctggt aaagaaacc ctctcggac 
acaatctcc gattttttg aaagattgt catcttttg 
aaaagtaag atattaggg cttgaacca gaacttgta 
aaatcagcg gaagtaaaa aactcagcc gctgataac 
gaggcagaa agcgaagtt gaatgtgaa cgtgagttt 
gtgcctgaa gtggactgt ggtaggcct ctgccgaaa 
ctgtacgac ccaagtggc gactttttg gacgattct 
gtgctagat tccgaaatt atcgaagat aaagatgaa 
ggaaatgag aaggatatt gagcacacg ggctcagaa 
gtgaagact gatcaaacg gacgaaacc cgcgagaag 
tttgactcg ccggaaaaa tccgaatgt gatagcgtt 
ctggaatca gaaactgaa aaacatgat aatactcca 
gaggagtcg ccaaaaatt gctactcca gaggaatct 
ccaaaggtt gtagaaccg attgaggaa gaaccaaga 
gctgagtat ttgctcgca tcgtgtgga aaagacaaa 
agtgtttac atctggcgg gcggggact gacggtaga 
atgcagacg ttcttccaa gccgctaaa aagggcaac 
aatcgcagg aagaacgaa agcacttgg atgtccctg 
tgttggatt gccccagat ttattactg acctcgtca 
acaaaggac gaacttcta gcctggcct ttgcccaaa 
cccaaaaca cccaagcaa taccgagtt gtccacctt 
gaacactca tccctcatt ttcaacatc gtggcccct 
cccaagtac acgacggcg ttcaattgg cgcgaaaaa 
cacgaactt agtgcgtgg acttggagc caagacgga 
aacttggtt tgttttgac ttggacgcg gaaaaaaca 
cggatgtgt ttttcgtgc ataactgcg ttcatcact 
tgttgcgat acttcacct ttggatccc aatcgactt 
gcgatcggt ttgaaagac ggtggtatc aaaatctgg 
gatttgagt ctcccacat actaaaact atcacaatg 
cagaaatac catcccaaa tatacgcac aaagttacc 
acgattgcc tggcaccct accagggaa atgtttcta 
gggttcggg actgaggaa ggacgcgtc ggctcgatt 
gacctcatt tcgaagaaa acaacgctt ttttctcac 
tactttcac agccaagtg tataaagtc gagtggggg 
cctttgaat gcggactta cacaactta gggctttat 
gcggtggct gagggcaag attatcatt tatgacgta 
tcaaacccc gaccaaggg ccgtctttc ttggacctt 
cccaacaac ttgtacgtc tataaattc gcctggaag 
ccggacttt cgtttgctt ttgttgaac ggaaaagac 
ggttctttg ttggtgcta acgatcgat ttagtggcc 
attactacg ctttatccg aacgaaaaa ggccttaac 
ggaatcgtg tggcatcct gattctttc atggacaaa 
accgacgtt tcccctcgt tgtaactgg tttgcagcg 
accacagtt aaagggatc ttggtctac gattgcggg 
tcaatccga gaaagtgac aattttgcc gaaaacatt 
gtcgctacg tttgaggga catgtgaag ccgcctttg 
gccttagcg tggtgtctt tttgatggt aatagaatc 
gcgtcgact gcttcggat ggactggtc catgtgtgg 
gacgtgtca acgaaatca gcagttgcg acttttgtt 
gacttctgc ctgagtcat aatccggca gttgtttgg 
agtccgatc gatccagat ttgattatt acaggtgat 
aggatgatt caggtttgg agagtttcc aaaaatccg 
ccaaaattg gacgaagag tttctagct gagaggaaa 
cgtcttgtg gctaaaatg gccaaaagc gatgacaaa 
gttgacaaa agtcgattt ttaattgag gtgccgaaa 
gccaaaact ccagctaaa aactacata gtgcctagt 
ttttacgta tcaaatgat gagaacctg gaaatgcaa 
gtgggcaat atgaaaaag ttgttagac caagttgaa 
aataaaaat acaggtgat ggcaagtta gggcccctt 
gagttattt gggtccaag gaacaagtg tcgcaactt 
ttagacaca aattataat caacagaaa aaagagggt 
aaatttaaa tcttgtgag ttgatttcg ctgtggaaa 
ggggacgta ggtgattat attcaaact gccattgat 
gagaatcgc gtcaacccc acgactatt aatttggcc 
gcaatggtt tcgccaaaa ttatggcaa gatgcgtgt 
ctgtcatac gcgaaaaaa ttgtcagag gatgccgcg 
tctgatccg ttcgagaca gcaatgtac tttttagcg 
tgccataaa atcgaagaa gcgataaat tcactgtgc 
accagtctc atgtttaag gaagcttta gttttggcc 
aaacagagg cttccggaa aatagcgtg tttatcacg 
gaagtgacg caaaaatgg gccgaacat tgtgtaacc 
accggcaat ttcgaaaat gctgctttt tgttatatt 
tcgattaag gaatatgaa aatgccgcc aaggtttta 
gcgcgaagg aatcataag gaatttctc gattttgca 
gccgaattg gccgaaaaa gcgggaaat aaaggactg 
catgactcg attaaatat aagtgtagt gaactggaa 
aatactcca caaaatggt gcaggtgac gaaatgccg 
tctcgaatt gatgccgtt ttggagaaa atgactgag 
aatcaagat gcagtttca gagagtggc tctgaaatt 
accaatgga tctcacccg gaatcagcc cataatgaa 
aattag
    mRNA sequence
    • >TC008111 RA
      gtcttgttc agtgcacaa actcgccca accacatgt 
gaaaataac gtgtaaatg cgttttaaa agttacttg 
ttcagttgt tgaataatt tggtgagat gaacgagtt 
agctgttcc tcctgcgcc cctttggta caggagttc 
agtgttggc gtgtgcccc tgacaatac cttggtgta 
tggcgcaaa aagcgatat tgttatcgt tagggaaaa 
aggccccga agaaccggc cgaagtgaa attgattca 
ttcggctca ctctaaact tctcacttc cgttagttt 
gaacaggag atggaagga gccccattc gtacgtggt 
gtccctggg ggaagacag cgtgatgaa ggtgtggga 
cttggatac cttcgagaa gaaaacgtc gaataggga 
tcatgtgga acacaaaaa ccccgtaat tggggccgt 
ttttgcggg tgatgatcg cgtcgtaag tgcgtcatc 
gaccggttt tgtacacgt ctggaatat caacacgaa 
tgagtccaa gcctttggt agatttatt caaaagtaa 
agtcactct gacttgtct ttcgtcgtg cccccatgc 
cggctggct catggcctt tggactaaa taacggtgt 
ggtgatcgt ggccgattt gcgtcgcaa tgggcaagt 
tttgtataa attgagggg gcattatca ggcggttgt 
ttgcttgtc ttggtgtcc agcacccgt aaatatctt 
tccgagaca tggccagaa tacggttaa aaataaatc 
aagcgaaag tgagcacaa tgagggttc tagttcaga 
taagacagt tgagaccaa agaaaaggt caatccttg 
ggtaaatct agttcatgc agatgatgg cgaagcctc 
atctggtaa agaaaccct ctcggacac aatctccga 
ttttttgaa agattgtca tcttttgaa aagtaagat 
attagggct tgaaccaga acttgtaaa atcagcgga 
agtaaaaaa ctcagccgc tgataacga ggcagaaag 
cgaagttga atgtgaacg tgagtttgt gcctgaagt 
ggactgtgg taggcctct gccgaaact gtacgaccc 
aagtggcga ctttttgga cgattctgt gctagattc 
cgaaattat cgaagataa agatgaagg aaatgagaa 
ggatattga gcacacggg ctcagaagt gaagactga 
tcaaacgga cgaaacccg cgagaagtt tgactcgcc 
ggaaaaatc cgaatgtga tagcgttct ggaatcaga 
aactgaaaa acatgataa tactccaga ggagtcgcc 
aaaaattgc tactccaga ggaatctcc aaaggttgt 
agaaccgat tgaggaaga accaagagc tgagtattt 
gctcgcatc gtgtggaaa agacaaaag tgtttacat 
ctggcgggc ggggactga cggtagaat gcagacgtt 
cttccaagc cgctaaaaa gggcaacaa tcgcaggaa 
gaacgaaag cacttggat gtccctgtg ttggattgc 
cccagattt attactgac ctcgtcaac aaaggacga 
acttctagc ctggccttt gcccaaacc caaaacacc 
caagcaata ccgagttgt ccaccttga acactcatc 
cctcatttt caacatcgt ggcccctcc caagtacac 
gacggcgtt caattggcg cgaaaaaca cgaacttag 
tgcgtggac ttggagcca agacggaaa cttggtttg 
ttttgactt ggacgcgga aaaaacacg gatgtgttt 
ttcgtgcat aactgcgtt catcacttg ttgcgatac 
ttcaccttt ggatcccaa tcgacttgc gatcggttt 
gaaagacgg tggtatcaa aatctggga tttgagtct 
cccacatac taaaactat cacaatgca gaaatacca 
tcccaaata tacgcacaa agttaccac gattgcctg 
gcaccctac cagggaaat gtttctagg gttcgggac 
tgaggaagg acgcgtcgg ctcgattga cctcatttc 
gaagaaaac aacgctttt ttctcacta ctttcacag 
ccaagtgta taaagtcga gtgggggcc tttgaatgc 
ggacttaca caacttagg gctttatgc ggtggctga 
gggcaagat tatcattta tgacgtatc aaaccccga 
ccaagggcc gtctttctt ggaccttcc caacaactt 
gtacgtcta taaattcgc ctggaagcc ggactttcg 
tttgctttt gttgaacgg aaaagacgg ttctttgtt 
ggtgctaac gatcgattt agtggccat tactacgct 
ttatccgaa cgaaaaagg ccttaacgg aatcgtgtg 
gcatcctga ttctttcat ggacaaaac cgacgtttc 
ccctcgttg taactggtt tgcagcgac cacagttaa 
agggatctt ggtctacga ttgcgggtc aatccgaga 
aagtgacaa ttttgccga aaacattgt cgctacgtt 
tgagggaca tgtgaagcc gcctttggc cttagcgtg 
gtgtctttt tgatggtaa tagaatcgc gtcgactgc 
ttcggatgg actggtcca tgtgtggga cgtgtcaac 
gaaatcagc agttgcgac ttttgttga cttctgcct 
gagtcataa tccggcagt tgtttggag tccgatcga 
tccagattt gattattac aggtgatag gatgattca 
ggtttggag agtttccaa aaatccgcc aaaattgga 
cgaagagtt tctagctga gaggaaacg tcttgtggc 
taaaatggc caaaagcga tgacaaagt tgacaaaag 
tcgattttt aattgaggt gccgaaagc caaaactcc 
agctaaaaa ctacatagt gcctagttt ttacgtatc 
aaatgatga gaacctgga aatgcaagt gggcaatat 
gaaaaagtt gttagacca agttgaaaa taaaaatac 
aggtgatgg caagttagg gccccttga gttatttgg 
gtccaagga acaagtgtc gcaactttt agacacaaa 
ttataatca acagaaaaa agagggtaa atttaaatc 
ttgtgagtt gatttcgct gtggaaagg ggacgtagg 
tgattatat tcaaactgc cattgatga gaatcgcgt 
caaccccac gactattaa tttggccgc aatggtttc 
gccaaaatt atggcaaga tgcgtgtct gtcatacgc 
gaaaaaatt gtcagagga tgccgcgtc tgatccgtt 
cgagacagc aatgtactt tttagcgtg ccataaaat 
cgaagaagc gataaattc actgtgcac cagtctcat 
gtttaagga agctttagt tttggccaa acagaggct 
tccggaaaa tagcgtgtt tatcacgga agtgacgca 
aaaatgggc cgaacattg tgtaaccac cggcaattt 
cgaaaatgc tgctttttg ttatatttc gattaagga 
atatgaaaa tgccgccaa ggttttagc gcgaaggaa 
tcataagga atttctcga ttttgcagc cgaattggc 
cgaaaaagc gggaaataa aggactgca tgactcgat 
taaatataa gtgtagtga actggaaaa tactccaca 
aaatggtgc aggtgacga aatgccgtc tcgaattga 
tgccgtttt ggagaaaat gactgagaa tcaagatgc 
agtttcaga gagtggctc tgaaattac caatggatc 
tcacccgga atcagccca taatgaaaa ttaggcgcc 
actttaggc gtgatagaa gattgttta agttttaag 
actgaacca accgaatag tattttaat ttttatagt 
ttttgcttt gttttttat ttttgtttc gtttgtttt 
ctaagaata aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY