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Gene information
General information
TC008092 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4974413..4975941
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008092
    iBeetle screen iB_06247

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    remarks: eggs lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008092 RA
      MSIKSEPAE PEMIIIKQE SDAAPASPC TATFFKEEK 
      TEDEPDESF QCKICKKRF ESEDELYEH ENAKTADRS 
      HSCCACGKS FRDNTQLNV HTRKHTGEK PYECSECGK 
      KFSINGNLS KHMRTHTGE KRFECDTCE RKFTQFAHL 
      EDHIKTHSG DRPFECDVC KSAFKTKAR LRKHRKSHE 
      DTTLTRRSV QCPICSKMM KNMRQLTNH MDCHSSEKN 
      LFQCDICGK SYHNLYYLQ NHQKTHSGV RPFKCTLCE 
      KTFITSGQL RRHQNTHTG YKPYNCDLC PKSFPNSQN 
      LRRHKLTHT GEKKHSCNI CGKSFIIVE NLTRHMRTH 
      TGEKPYSCD ICGRRFAHS TTVKEHRRT HTGDKPFGC 
      GICDKRFTI NKLLYKHIR AKHPEDFNS FKEQEMRKK 
      YVMFSVKSE PGIKIEKLD DT
    CDS sequence
    • >TC008092 RA
      atgtcgata aaatccgag ccggccgaa cccgaaatg 
      atcataatc aagcaggaa tcagacgcc gctcccgcc 
      tcgccctgc accgcgact tttttcaag gaagaaaag 
      accgaagac gaacccgac gagagtttc caatgcaaa 
      atttgcaaa aagcgattc gagagtgag gacgagttg 
      tacgagcac gaaaacgcc aaaacggct gataggtcg 
      cactcgtgt tgcgcctgt ggtaagtcg ttccgcgac 
      aacacccag ctgaacgtc cacacgcgg aagcacacg 
      ggggagaag ccctacgag tgcagcgaa tgcgggaaa 
      aaattctcg atcaacggc aatttgagc aaacatatg 
      cgcacccac acgggggaa aagcggttc gagtgcgat 
      acttgcgaa agaaagttt actcaattc gcgcacctg 
      gaagaccac atcaagacg cattcgggc gaccggccg 
      ttcgagtgc gacgtttgt aaaagcgcc ttcaagacg 
      aaagcccgg cttaggaag catagaaaa tcgcacgag 
      gacaccact ctaacccgg aggtcggtc cagtgcccc 
      atttgctcg aaaatgatg aaaaacatg cggcaatta 
      accaaccac atggactgt cattcctcc gagaaaaat 
      ttgttccag tgcgatatt tgcggcaaa agttatcac 
      aatttgtat tacttgcaa aaccaccaa aagacgcac 
      agtggcgtc cgccctttc aaatgcact ctctgtgag 
      aaaaccttc attacctcg ggccaactc aggcgccac 
      caaaacacc cacacgggc tacaagccc tataattgc 
      gatctctgt cccaagtca ttccccaac tcccagaat 
      ttgcgccga cataagctc acacatacc ggcgagaaa 
      aaacattct tgtaacatt tgcggcaag agttttatc 
      atcgttgag aatttgacc cgccatatg aggacccac 
      acgggggaa aagccctac tcgtgtgac atatgcggc 
      cgcaggttt gcccacagt acgacggtc aaagaacat 
      agaaggacc catacgggg gataagccc ttcgggtgc 
      ggcatttgt gataaaaga tttactatc aataaatta 
      ttgtacaaa catatacga gcgaaacat ccagaggat 
      tttaatagt ttcaaggaa caggaaatg aggaaaaag 
      tatgttatg tttagtgtg aaaagtgag cctgggatt 
      aaaattgaa aaattggac gacacgtaa
    mRNA sequence
    • >TC008092 RA
      acctgtcaa acacaaata aaatgaaaa aaattcgaa 
      aaaacaggt tcgggctca aacgtaacg tgtgcaata 
      acaagatat gtgtaaatt gattattcc caaacattt 
      gtgtgcgag ccactaaaa cctcctcac tacacgata 
      aacaaagag tgaggttat gcccgtgtt gtcccgcac 
      tccaaaacc tggatttcc gcaaaaaac ggcttgata 
      ttatgggaa atcattaac gtggtgcaa tgtcgataa 
      aatccgagc cggccgaac ccgaaatga tcataatca 
      agcaggaat cagacgccg ctcccgcct cgccctgca 
      ccgcgactt ttttcaagg aagaaaaga ccgaagacg 
      aacccgacg agagtttcc aatgcaaaa tttgcaaaa 
      agcgattcg agagtgagg acgagttgt acgagcacg 
      aaaacgcca aaacggctg ataggtcgc actcgtgtt 
      gcgcctgtg gtaagtcgt tccgcgaca acacccagc 
      tgaacgtcc acacgcgga agcacacgg gggagaagc 
      cctacgagt gcagcgaat gcgggaaaa aattctcga 
      tcaacggca atttgagca aacatatgc gcacccaca 
      cgggggaaa agcggttcg agtgcgata cttgcgaaa 
      gaaagttta ctcaattcg cgcacctgg aagaccaca 
      tcaagacgc attcgggcg accggccgt tcgagtgcg 
      acgtttgta aaagcgcct tcaagacga aagcccggc 
      ttaggaagc atagaaaat cgcacgagg acaccactc 
      taacccgga ggtcggtcc agtgcccca tttgctcga 
      aaatgatga aaaacatgc ggcaattaa ccaaccaca 
      tggactgtc attcctccg agaaaaatt tgttccagt 
      gcgatattt gcggcaaaa gttatcaca atttgtatt 
      acttgcaaa accaccaaa agacgcaca gtggcgtcc 
      gccctttca aatgcactc tctgtgaga aaaccttca 
      ttacctcgg gccaactca ggcgccacc aaaacaccc 
      acacgggct acaagccct ataattgcg atctctgtc 
      ccaagtcat tccccaact cccagaatt tgcgccgac 
      ataagctca cacataccg gcgagaaaa aacattctt 
      gtaacattt gcggcaaga gttttatca tcgttgaga 
      atttgaccc gccatatga ggacccaca cgggggaaa 
      agccctact cgtgtgaca tatgcggcc gcaggtttg 
      cccacagta cgacggtca aagaacata gaaggaccc 
      atacggggg ataagccct tcgggtgcg gcatttgtg 
      ataaaagat ttactatca ataaattat tgtacaaac 
      atatacgag cgaaacatc cagaggatt ttaatagtt 
      tcaaggaac aggaaatga ggaaaaagt atgttatgt 
      ttagtgtga aaagtgagc ctgggatta aaattgaaa 
      aattggacg acacgtaat gtgaataag gcttctaat 
      aaaaacctc caaaacct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY