Status
Please wait ...
Gene information
General information
TC008000 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3711256..3714297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008000
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06246

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008000 RA
      MGAPENRAI ILSDCGTVL SALSYKDVN SLRSSITQF 
      ISVHVTEKH QCIGLAMNH NIYIPSIIL SLQELHHCF 
      IFLCDETES ITTNLGLLW SLSFASGNN TVATLLLNG 
      QKLVLNKHK VTHVTNYPN IFYIIRTSG STGVSKFVR 
      VTHSSIERN VKHLSKIFK IGASDTIYF GTPLTFDPS 
      MIELLLALE NGACLLITP PKNQINPVR LHQTLFHPQ 
      NGATVLQIV PSLFLRWSE TQISDFLQK SKLKILAFG 
      GEPFPLSLL AYQKSDFLR IFNLYGVTE VSCWATVCE 
      VAEGLGEVP LGEELDDTI VELRDDFGR VVENGEGEI 
      FIGSSTRIC FVGDEKIHH KTPVFRATG DIGFISDKK 
      IYYKGRKND VFKRLGHKV QLSKIETMV LDETGLRNV 
      CVWSKTRTK LLLFLIVEN CDNSTKSKV LDKVRIKLL 
      HTLPKQYFP DFIDIIRKC PITSHGKTD KNSLESIYL 
      NRNRTDKRQ VDIFKTLIC KYFGVRNFD KNTFFELGG 
      NSILAVQFL SEFEDETGA ACPDLVTHL FESDLNTCI 
      DILRDVKVV QKRPQTEPP PKNEKKIKN SNIGIELKI 
      AWSYNLEAC VDSTPLVFS HNGRELVAV GAFSHIFAI 
      LDTQSGHEI AKFVLPDVV ESTPCYHNN YLYVTCFDG 
      NLYCLDFAS NSIKWAYKT NDRVKCTPI LCSSGARII 
      FGSYDKHIH CVDIMQGGV VWTSFVSES VISKPLLLN 
      QNIYVATIC GTCLCLSET SGAILWQYK TGSPIFGAP 
      CTHNGCILW PNVTGSVIC MALDGRKLW QFDTGGHLY 
      SSFARRDNF LFLGSHDRN LYVLKIESE APEPQFVLN 
      LDDCLTTPF ISGDFIIVA GNSGRLFVV TFRGELGAQ 
      LGLPGEVFS SVVGGDGRF FVGCRDNNL YCVEIKNS
    CDS sequence
    • >TC008000 RA
      atgggggct ccagaaaac cgagcgata attttgagt 
      gactgtggc accgttcta agtgctctt agttacaaa 
      gacgtcaat tcattacgt agttcgata acccagttc 
      atatcggtg catgtcaca gaaaaacac caatgcata 
      ggtcttgca atgaatcac aatatttac attccttcg 
      attatttta agtttacag gaattgcac cactgtttt 
      atttttttg tgtgacgaa actgagtca attacaaca 
      aatttgggg cttctctgg tcattatct ttcgcttcg 
      ggcaataac accgtcgcc actttatta ctaaatggc 
      caaaaactc gtcttgaac aaacacaaa gtgactcac 
      gtaacgaat tatccgaat attttttac ataattcga 
      acatcaggc agtacaggt gtgagcaag tttgtgagg 
      gttactcac tcaagtatt gaacgcaac gtcaaacat 
      ttgagtaaa atattcaaa atcggggct tccgatacg 
      atttacttc ggcaccccg ctcactttt gacccctca 
      atgatcgag ttgttgctc gccctcgaa aatggggct 
      tgtctctta ataacgccc cctaagaac caaatcaat 
      ccggtgcgt ctccaccag acgttgttc catccccaa 
      aatggggcc actgtcctc caaatcgtc ccgtctcta 
      tttctacga tggtcggag acacaaatc agtgatttt 
      ctccaaaaa tcaaaatta aaaatccta gcttttggg 
      ggcgaaccg ttcccttta tctcttctc gcttatcaa 
      aagtcggat tttttgcga atttttaat ttgtacgga 
      gtcactgaa gtgtcatgt tgggccact gtttgcgaa 
      gtcgcggaa ggtttaggg gaagtaccg ctcggggag 
      gaattagac gacactatt gtggagtta agggatgat 
      ttcggacgg gttgtggaa aatggtgaa ggagaaatt 
      ttcattggg agttcgacc cgaatttgc ttcgtgggt 
      gatgagaaa attcatcac aaaacacca gtttttcgg 
      gctactggt gacatcggg tttatttct gataagaag 
      atttattac aaagggcgc aaaaacgat gtttttaaa 
      cgcttgggc cataaagta caattaagt aaaattgaa 
      acaatggta ttggacgag acagggtta cggaacgtt 
      tgtgtgtgg tctaaaacc cgcacaaag cttttactt 
      tttttgata gtcgaaaac tgcgataac agtaccaaa 
      agcaaggtt ttagataag gtcaggatt aaactttta 
      cacacttta ccgaaacaa tacttccct gattttatc 
      gatatcatt cgaaagtgc ccaatcact tcgcatgga 
      aaaaccgat aagaactct ctcgaatcg atttatttg 
      aatcgaaat agaaccgat aagagacaa gttgatatt 
      tttaaaaca ctgatttgt aaatatttt ggtgttcga 
      aattttgat aaaaacaca tttttcgaa cttgggggc 
      aactcgatt cttgcagtg caatttttg agcgaattc 
      gaggacgaa acaggggca gcttgtccc gatttagtg 
      actcatttg tttgaaagt gacttaaac acctgtatt 
      gatatttta cgtgacgtt aaagtcgta caaaaaaga 
      ccacaaact gaaccgccc ccgaaaaat gagaaaaaa 
      ataaaaaat agcaacatc ggaattgaa ttaaaaatt 
      gcttggagc tataacttg gaggcttgc gtcgactca 
      acacctctc gttttttct cacaatgga cgcgagtta 
      gtcgccgtt ggggcgttt tcgcacatt tttgccatt 
      ttagacacg caaagtggc cacgaaatc gccaaattt 
      gtccttcca gacgtcgtc gaaagcact ccttgctac 
      cacaataac tacctatac gtgacttgc tttgatggc 
      aatttatac tgtcttgat ttcgcatca aattcaatc 
      aaatgggct tataagaca aatgacagg gtcaagtgc 
      accccaatt ttgtgctcc agtggcgca agaatcatt 
      ttcgggtct tacgataag cacatccat tgcgtcgat 
      attatgcaa ggaggcgtt gtttggacc tcgtttgtt 
      tccgaaagt gtgatttca aaacctcta cttttaaac 
      caaaacatt tacgtggcc acgatttgt ggcacttgt 
      ctctgcctt tcggaaact tccggtgct attttatgg 
      caatacaaa accggaagt ccgattttt ggggctccg 
      tgcacccac aacgggtgc attttatgg ccaaatgta 
      accggaagc gtcatatgt atggcttta gatggccgc 
      aaattatgg cagtttgat acagggggc catttatac 
      tcgtctttc gccagacga gacaatttt ctattttta 
      gggtcacat gatcgtaat ttgtacgtg ttaaaaatt 
      gaaagtgaa gcccctgag ccccaattc gtgcttaac 
      ttagatgat tgtttaaca actccgttc atttcgggc 
      gattttata attgtggcg gggaattcg ggtcgtttg 
      tttgtggtg acgtttagg ggtgagttg ggggctcaa 
      ttggggttg ccgggggag gttttttcg tcggttgtg 
      ggcggtgac ggacgattt tttgtaggg tgtcgtgat 
      aataacttg tattgtgtt gaaattaaa aacagttga
    mRNA sequence
    • >TC008000 RA
      aaaacacaa aaaattgca aagacacag accaaaaaa 
      ttttgcaaa tgatttgtg cctaacttc cctccgctg 
      acataaccc aagtttttc aaaaatggg ggctccaga 
      aaaccgagc gataatttt gagtgactg tggcaccgt 
      tctaagtgc tcttagtta caaagacgt caattcatt 
      acgtagttc gataaccca gttcatatc ggtgcatgt 
      cacagaaaa acaccaatg cataggtct tgcaatgaa 
      tcacaatat ttacattcc ttcgattat tttaagttt 
      acaggaatt gcaccactg ttttatttt tttgtgtga 
      cgaaactga gtcaattac aacaaattt ggggcttct 
      ctggtcatt atctttcgc ttcgggcaa taacaccgt 
      cgccacttt attactaaa tggccaaaa actcgtctt 
      gaacaaaca caaagtgac tcacgtaac gaattatcc 
      gaatatttt ttacataat tcgaacatc aggcagtac 
      aggtgtgag caagtttgt gagggttac tcactcaag 
      tattgaacg caacgtcaa acatttgag taaaatatt 
      caaaatcgg ggcttccga tacgattta cttcggcac 
      cccgctcac ttttgaccc ctcaatgat cgagttgtt 
      gctcgccct cgaaaatgg ggcttgtct cttaataac 
      gccccctaa gaaccaaat caatccggt gcgtctcca 
      ccagacgtt gttccatcc ccaaaatgg ggccactgt 
      cctccaaat cgtcccgtc tctatttct acgatggtc 
      ggagacaca aatcagtga ttttctcca aaaatcaaa 
      attaaaaat cctagcttt tgggggcga accgttccc 
      tttatctct tctcgctta tcaaaagtc ggatttttt 
      gcgaatttt taatttgta cggagtcac tgaagtgtc 
      atgttgggc cactgtttg cgaagtcgc ggaaggttt 
      aggggaagt accgctcgg ggaggaatt agacgacac 
      tattgtgga gttaaggga tgatttcgg acgggttgt 
      ggaaaatgg tgaaggaga aattttcat tgggagttc 
      gacccgaat ttgcttcgt gggtgatga gaaaattca 
      tcacaaaac accagtttt tcgggctac tggtgacat 
      cgggtttat ttctgataa gaagattta ttacaaagg 
      gcgcaaaaa cgatgtttt taaacgctt gggccataa 
      agtacaatt aagtaaaat tgaaacaat ggtattgga 
      cgagacagg gttacggaa cgtttgtgt gtggtctaa 
      aacccgcac aaagctttt acttttttt gatagtcga 
      aaactgcga taacagtac caaaagcaa ggttttaga 
      taaggtcag gattaaact tttacacac tttaccgaa 
      acaatactt ccctgattt tatcgatat cattcgaaa 
      gtgcccaat cacttcgca tggaaaaac cgataagaa 
      ctctctcga atcgattta tttgaatcg aaatagaac 
      cgataagag acaagttga tatttttaa aacactgat 
      ttgtaaata ttttggtgt tcgaaattt tgataaaaa 
      cacattttt cgaacttgg gggcaactc gattcttgc 
      agtgcaatt tttgagcga attcgagga cgaaacagg 
      ggcagcttg tcccgattt agtgactca tttgtttga 
      aagtgactt aaacacctg tattgatat tttacgtga 
      cgttaaagt cgtacaaaa aagaccaca aactgaacc 
      gcccccgaa aaatgagaa aaaaataaa aaatagcaa 
      catcggaat tgaattaaa aattgcttg gagctataa 
      cttggaggc ttgcgtcga ctcaacacc tctcgtttt 
      ttctcacaa tggacgcga gttagtcgc cgttggggc 
      gttttcgca catttttgc cattttaga cacgcaaag 
      tggccacga aatcgccaa atttgtcct tccagacgt 
      cgtcgaaag cactccttg ctaccacaa taactacct 
      atacgtgac ttgctttga tggcaattt atactgtct 
      tgatttcgc atcaaattc aatcaaatg ggcttataa 
      gacaaatga cagggtcaa gtgcacccc aattttgtg 
      ctccagtgg cgcaagaat cattttcgg gtcttacga 
      taagcacat ccattgcgt cgatattat gcaaggagg 
      cgttgtttg gacctcgtt tgtttccga aagtgtgat 
      ttcaaaacc tctactttt aaaccaaaa catttacgt 
      ggccacgat ttgtggcac ttgtctctg cctttcgga 
      aacttccgg tgctatttt atggcaata caaaaccgg 
      aagtccgat ttttggggc tccgtgcac ccacaacgg 
      gtgcatttt atggccaaa tgtaaccgg aagcgtcat 
      atgtatggc tttagatgg ccgcaaatt atggcagtt 
      tgatacagg gggccattt atactcgtc tttcgccag 
      acgagacaa ttttctatt tttagggtc acatgatcg 
      taatttgta cgtgttaaa aattgaaag tgaagcccc 
      tgagcccca attcgtgct taacttaga tgattgttt 
      aacaactcc gttcatttc gggcgattt tataattgt 
      ggcggggaa ttcgggtcg tttgtttgt ggtgacgtt 
      taggggtga gttgggggc tcaattggg gttgccggg 
      ggaggtttt ttcgtcggt tgtgggcgg tgacggacg 
      attttttgt agggtgtcg tgataataa cttgtattg 
      tgttgaaat taaaaacag ttgacttga acttgattg 
      tttccgatt tttgttggt ttgttaatt aaacaatgt 
      acgaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY