Status
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Gene information
General information
TC008000 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3711256..3714297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008000
    iBeetle screen iB_06246

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008000 RA
      MGAPENRAI ILSDCGTVL SALSYKDVN SLRSSITQF 
ISVHVTEKH QCIGLAMNH NIYIPSIIL SLQELHHCF 
IFLCDETES ITTNLGLLW SLSFASGNN TVATLLLNG 
QKLVLNKHK VTHVTNYPN IFYIIRTSG STGVSKFVR 
VTHSSIERN VKHLSKIFK IGASDTIYF GTPLTFDPS 
MIELLLALE NGACLLITP PKNQINPVR LHQTLFHPQ 
NGATVLQIV PSLFLRWSE TQISDFLQK SKLKILAFG 
GEPFPLSLL AYQKSDFLR IFNLYGVTE VSCWATVCE 
VAEGLGEVP LGEELDDTI VELRDDFGR VVENGEGEI 
FIGSSTRIC FVGDEKIHH KTPVFRATG DIGFISDKK 
IYYKGRKND VFKRLGHKV QLSKIETMV LDETGLRNV 
CVWSKTRTK LLLFLIVEN CDNSTKSKV LDKVRIKLL 
HTLPKQYFP DFIDIIRKC PITSHGKTD KNSLESIYL 
NRNRTDKRQ VDIFKTLIC KYFGVRNFD KNTFFELGG 
NSILAVQFL SEFEDETGA ACPDLVTHL FESDLNTCI 
DILRDVKVV QKRPQTEPP PKNEKKIKN SNIGIELKI 
AWSYNLEAC VDSTPLVFS HNGRELVAV GAFSHIFAI 
LDTQSGHEI AKFVLPDVV ESTPCYHNN YLYVTCFDG 
NLYCLDFAS NSIKWAYKT NDRVKCTPI LCSSGARII 
FGSYDKHIH CVDIMQGGV VWTSFVSES VISKPLLLN 
QNIYVATIC GTCLCLSET SGAILWQYK TGSPIFGAP 
CTHNGCILW PNVTGSVIC MALDGRKLW QFDTGGHLY 
SSFARRDNF LFLGSHDRN LYVLKIESE APEPQFVLN 
LDDCLTTPF ISGDFIIVA GNSGRLFVV TFRGELGAQ 
LGLPGEVFS SVVGGDGRF FVGCRDNNL YCVEIKNS
    CDS sequence
    • >TC008000 RA
      atgggggct ccagaaaac cgagcgata attttgagt 
gactgtggc accgttcta agtgctctt agttacaaa 
gacgtcaat tcattacgt agttcgata acccagttc 
atatcggtg catgtcaca gaaaaacac caatgcata 
ggtcttgca atgaatcac aatatttac attccttcg 
attatttta agtttacag gaattgcac cactgtttt 
atttttttg tgtgacgaa actgagtca attacaaca 
aatttgggg cttctctgg tcattatct ttcgcttcg 
ggcaataac accgtcgcc actttatta ctaaatggc 
caaaaactc gtcttgaac aaacacaaa gtgactcac 
gtaacgaat tatccgaat attttttac ataattcga 
acatcaggc agtacaggt gtgagcaag tttgtgagg 
gttactcac tcaagtatt gaacgcaac gtcaaacat 
ttgagtaaa atattcaaa atcggggct tccgatacg 
atttacttc ggcaccccg ctcactttt gacccctca 
atgatcgag ttgttgctc gccctcgaa aatggggct 
tgtctctta ataacgccc cctaagaac caaatcaat 
ccggtgcgt ctccaccag acgttgttc catccccaa 
aatggggcc actgtcctc caaatcgtc ccgtctcta 
tttctacga tggtcggag acacaaatc agtgatttt 
ctccaaaaa tcaaaatta aaaatccta gcttttggg 
ggcgaaccg ttcccttta tctcttctc gcttatcaa 
aagtcggat tttttgcga atttttaat ttgtacgga 
gtcactgaa gtgtcatgt tgggccact gtttgcgaa 
gtcgcggaa ggtttaggg gaagtaccg ctcggggag 
gaattagac gacactatt gtggagtta agggatgat 
ttcggacgg gttgtggaa aatggtgaa ggagaaatt 
ttcattggg agttcgacc cgaatttgc ttcgtgggt 
gatgagaaa attcatcac aaaacacca gtttttcgg 
gctactggt gacatcggg tttatttct gataagaag 
atttattac aaagggcgc aaaaacgat gtttttaaa 
cgcttgggc cataaagta caattaagt aaaattgaa 
acaatggta ttggacgag acagggtta cggaacgtt 
tgtgtgtgg tctaaaacc cgcacaaag cttttactt 
tttttgata gtcgaaaac tgcgataac agtaccaaa 
agcaaggtt ttagataag gtcaggatt aaactttta 
cacacttta ccgaaacaa tacttccct gattttatc 
gatatcatt cgaaagtgc ccaatcact tcgcatgga 
aaaaccgat aagaactct ctcgaatcg atttatttg 
aatcgaaat agaaccgat aagagacaa gttgatatt 
tttaaaaca ctgatttgt aaatatttt ggtgttcga 
aattttgat aaaaacaca tttttcgaa cttgggggc 
aactcgatt cttgcagtg caatttttg agcgaattc 
gaggacgaa acaggggca gcttgtccc gatttagtg 
actcatttg tttgaaagt gacttaaac acctgtatt 
gatatttta cgtgacgtt aaagtcgta caaaaaaga 
ccacaaact gaaccgccc ccgaaaaat gagaaaaaa 
ataaaaaat agcaacatc ggaattgaa ttaaaaatt 
gcttggagc tataacttg gaggcttgc gtcgactca 
acacctctc gttttttct cacaatgga cgcgagtta 
gtcgccgtt ggggcgttt tcgcacatt tttgccatt 
ttagacacg caaagtggc cacgaaatc gccaaattt 
gtccttcca gacgtcgtc gaaagcact ccttgctac 
cacaataac tacctatac gtgacttgc tttgatggc 
aatttatac tgtcttgat ttcgcatca aattcaatc 
aaatgggct tataagaca aatgacagg gtcaagtgc 
accccaatt ttgtgctcc agtggcgca agaatcatt 
ttcgggtct tacgataag cacatccat tgcgtcgat 
attatgcaa ggaggcgtt gtttggacc tcgtttgtt 
tccgaaagt gtgatttca aaacctcta cttttaaac 
caaaacatt tacgtggcc acgatttgt ggcacttgt 
ctctgcctt tcggaaact tccggtgct attttatgg 
caatacaaa accggaagt ccgattttt ggggctccg 
tgcacccac aacgggtgc attttatgg ccaaatgta 
accggaagc gtcatatgt atggcttta gatggccgc 
aaattatgg cagtttgat acagggggc catttatac 
tcgtctttc gccagacga gacaatttt ctattttta 
gggtcacat gatcgtaat ttgtacgtg ttaaaaatt 
gaaagtgaa gcccctgag ccccaattc gtgcttaac 
ttagatgat tgtttaaca actccgttc atttcgggc 
gattttata attgtggcg gggaattcg ggtcgtttg 
tttgtggtg acgtttagg ggtgagttg ggggctcaa 
ttggggttg ccgggggag gttttttcg tcggttgtg 
ggcggtgac ggacgattt tttgtaggg tgtcgtgat 
aataacttg tattgtgtt gaaattaaa aacagttga
    mRNA sequence
    • >TC008000 RA
      aaaacacaa aaaattgca aagacacag accaaaaaa 
ttttgcaaa tgatttgtg cctaacttc cctccgctg 
acataaccc aagtttttc aaaaatggg ggctccaga 
aaaccgagc gataatttt gagtgactg tggcaccgt 
tctaagtgc tcttagtta caaagacgt caattcatt 
acgtagttc gataaccca gttcatatc ggtgcatgt 
cacagaaaa acaccaatg cataggtct tgcaatgaa 
tcacaatat ttacattcc ttcgattat tttaagttt 
acaggaatt gcaccactg ttttatttt tttgtgtga 
cgaaactga gtcaattac aacaaattt ggggcttct 
ctggtcatt atctttcgc ttcgggcaa taacaccgt 
cgccacttt attactaaa tggccaaaa actcgtctt 
gaacaaaca caaagtgac tcacgtaac gaattatcc 
gaatatttt ttacataat tcgaacatc aggcagtac 
aggtgtgag caagtttgt gagggttac tcactcaag 
tattgaacg caacgtcaa acatttgag taaaatatt 
caaaatcgg ggcttccga tacgattta cttcggcac 
cccgctcac ttttgaccc ctcaatgat cgagttgtt 
gctcgccct cgaaaatgg ggcttgtct cttaataac 
gccccctaa gaaccaaat caatccggt gcgtctcca 
ccagacgtt gttccatcc ccaaaatgg ggccactgt 
cctccaaat cgtcccgtc tctatttct acgatggtc 
ggagacaca aatcagtga ttttctcca aaaatcaaa 
attaaaaat cctagcttt tgggggcga accgttccc 
tttatctct tctcgctta tcaaaagtc ggatttttt 
gcgaatttt taatttgta cggagtcac tgaagtgtc 
atgttgggc cactgtttg cgaagtcgc ggaaggttt 
aggggaagt accgctcgg ggaggaatt agacgacac 
tattgtgga gttaaggga tgatttcgg acgggttgt 
ggaaaatgg tgaaggaga aattttcat tgggagttc 
gacccgaat ttgcttcgt gggtgatga gaaaattca 
tcacaaaac accagtttt tcgggctac tggtgacat 
cgggtttat ttctgataa gaagattta ttacaaagg 
gcgcaaaaa cgatgtttt taaacgctt gggccataa 
agtacaatt aagtaaaat tgaaacaat ggtattgga 
cgagacagg gttacggaa cgtttgtgt gtggtctaa 
aacccgcac aaagctttt acttttttt gatagtcga 
aaactgcga taacagtac caaaagcaa ggttttaga 
taaggtcag gattaaact tttacacac tttaccgaa 
acaatactt ccctgattt tatcgatat cattcgaaa 
gtgcccaat cacttcgca tggaaaaac cgataagaa 
ctctctcga atcgattta tttgaatcg aaatagaac 
cgataagag acaagttga tatttttaa aacactgat 
ttgtaaata ttttggtgt tcgaaattt tgataaaaa 
cacattttt cgaacttgg gggcaactc gattcttgc 
agtgcaatt tttgagcga attcgagga cgaaacagg 
ggcagcttg tcccgattt agtgactca tttgtttga 
aagtgactt aaacacctg tattgatat tttacgtga 
cgttaaagt cgtacaaaa aagaccaca aactgaacc 
gcccccgaa aaatgagaa aaaaataaa aaatagcaa 
catcggaat tgaattaaa aattgcttg gagctataa 
cttggaggc ttgcgtcga ctcaacacc tctcgtttt 
ttctcacaa tggacgcga gttagtcgc cgttggggc 
gttttcgca catttttgc cattttaga cacgcaaag 
tggccacga aatcgccaa atttgtcct tccagacgt 
cgtcgaaag cactccttg ctaccacaa taactacct 
atacgtgac ttgctttga tggcaattt atactgtct 
tgatttcgc atcaaattc aatcaaatg ggcttataa 
gacaaatga cagggtcaa gtgcacccc aattttgtg 
ctccagtgg cgcaagaat cattttcgg gtcttacga 
taagcacat ccattgcgt cgatattat gcaaggagg 
cgttgtttg gacctcgtt tgtttccga aagtgtgat 
ttcaaaacc tctactttt aaaccaaaa catttacgt 
ggccacgat ttgtggcac ttgtctctg cctttcgga 
aacttccgg tgctatttt atggcaata caaaaccgg 
aagtccgat ttttggggc tccgtgcac ccacaacgg 
gtgcatttt atggccaaa tgtaaccgg aagcgtcat 
atgtatggc tttagatgg ccgcaaatt atggcagtt 
tgatacagg gggccattt atactcgtc tttcgccag 
acgagacaa ttttctatt tttagggtc acatgatcg 
taatttgta cgtgttaaa aattgaaag tgaagcccc 
tgagcccca attcgtgct taacttaga tgattgttt 
aacaactcc gttcatttc gggcgattt tataattgt 
ggcggggaa ttcgggtcg tttgtttgt ggtgacgtt 
taggggtga gttgggggc tcaattggg gttgccggg 
ggaggtttt ttcgtcggt tgtgggcgg tgacggacg 
attttttgt agggtgtcg tgataataa cttgtattg 
tgttgaaat taaaaacag ttgacttga acttgattg 
tttccgatt tttgttggt ttgttaatt aaacaatgt 
acgaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY