| General information | |||
| TC008000 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3711256..3714297 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008000 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06246 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC008000 RA
MGAPENRAI ILSDCGTVL SALSYKDVN SLRSSITQF ISVHVTEKH QCIGLAMNH NIYIPSIIL SLQELHHCF IFLCDETES ITTNLGLLW SLSFASGNN TVATLLLNG QKLVLNKHK VTHVTNYPN IFYIIRTSG STGVSKFVR VTHSSIERN VKHLSKIFK IGASDTIYF GTPLTFDPS MIELLLALE NGACLLITP PKNQINPVR LHQTLFHPQ NGATVLQIV PSLFLRWSE TQISDFLQK SKLKILAFG GEPFPLSLL AYQKSDFLR IFNLYGVTE VSCWATVCE VAEGLGEVP LGEELDDTI VELRDDFGR VVENGEGEI FIGSSTRIC FVGDEKIHH KTPVFRATG DIGFISDKK IYYKGRKND VFKRLGHKV QLSKIETMV LDETGLRNV CVWSKTRTK LLLFLIVEN CDNSTKSKV LDKVRIKLL HTLPKQYFP DFIDIIRKC PITSHGKTD KNSLESIYL NRNRTDKRQ VDIFKTLIC KYFGVRNFD KNTFFELGG NSILAVQFL SEFEDETGA ACPDLVTHL FESDLNTCI DILRDVKVV QKRPQTEPP PKNEKKIKN SNIGIELKI AWSYNLEAC VDSTPLVFS HNGRELVAV GAFSHIFAI LDTQSGHEI AKFVLPDVV ESTPCYHNN YLYVTCFDG NLYCLDFAS NSIKWAYKT NDRVKCTPI LCSSGARII FGSYDKHIH CVDIMQGGV VWTSFVSES VISKPLLLN QNIYVATIC GTCLCLSET SGAILWQYK TGSPIFGAP CTHNGCILW PNVTGSVIC MALDGRKLW QFDTGGHLY SSFARRDNF LFLGSHDRN LYVLKIESE APEPQFVLN LDDCLTTPF ISGDFIIVA GNSGRLFVV TFRGELGAQ LGLPGEVFS SVVGGDGRF FVGCRDNNL YCVEIKNS
- >TC008000 RA
atgggggct ccagaaaac cgagcgata attttgagt gactgtggc accgttcta agtgctctt agttacaaa gacgtcaat tcattacgt agttcgata acccagttc atatcggtg catgtcaca gaaaaacac caatgcata ggtcttgca atgaatcac aatatttac attccttcg attatttta agtttacag gaattgcac cactgtttt atttttttg tgtgacgaa actgagtca attacaaca aatttgggg cttctctgg tcattatct ttcgcttcg ggcaataac accgtcgcc actttatta ctaaatggc caaaaactc gtcttgaac aaacacaaa gtgactcac gtaacgaat tatccgaat attttttac ataattcga acatcaggc agtacaggt gtgagcaag tttgtgagg gttactcac tcaagtatt gaacgcaac gtcaaacat ttgagtaaa atattcaaa atcggggct tccgatacg atttacttc ggcaccccg ctcactttt gacccctca atgatcgag ttgttgctc gccctcgaa aatggggct tgtctctta ataacgccc cctaagaac caaatcaat ccggtgcgt ctccaccag acgttgttc catccccaa aatggggcc actgtcctc caaatcgtc ccgtctcta tttctacga tggtcggag acacaaatc agtgatttt ctccaaaaa tcaaaatta aaaatccta gcttttggg ggcgaaccg ttcccttta tctcttctc gcttatcaa aagtcggat tttttgcga atttttaat ttgtacgga gtcactgaa gtgtcatgt tgggccact gtttgcgaa gtcgcggaa ggtttaggg gaagtaccg ctcggggag gaattagac gacactatt gtggagtta agggatgat ttcggacgg gttgtggaa aatggtgaa ggagaaatt ttcattggg agttcgacc cgaatttgc ttcgtgggt gatgagaaa attcatcac aaaacacca gtttttcgg gctactggt gacatcggg tttatttct gataagaag atttattac aaagggcgc aaaaacgat gtttttaaa cgcttgggc cataaagta caattaagt aaaattgaa acaatggta ttggacgag acagggtta cggaacgtt tgtgtgtgg tctaaaacc cgcacaaag cttttactt tttttgata gtcgaaaac tgcgataac agtaccaaa agcaaggtt ttagataag gtcaggatt aaactttta cacacttta ccgaaacaa tacttccct gattttatc gatatcatt cgaaagtgc ccaatcact tcgcatgga aaaaccgat aagaactct ctcgaatcg atttatttg aatcgaaat agaaccgat aagagacaa gttgatatt tttaaaaca ctgatttgt aaatatttt ggtgttcga aattttgat aaaaacaca tttttcgaa cttgggggc aactcgatt cttgcagtg caatttttg agcgaattc gaggacgaa acaggggca gcttgtccc gatttagtg actcatttg tttgaaagt gacttaaac acctgtatt gatatttta cgtgacgtt aaagtcgta caaaaaaga ccacaaact gaaccgccc ccgaaaaat gagaaaaaa ataaaaaat agcaacatc ggaattgaa ttaaaaatt gcttggagc tataacttg gaggcttgc gtcgactca acacctctc gttttttct cacaatgga cgcgagtta gtcgccgtt ggggcgttt tcgcacatt tttgccatt ttagacacg caaagtggc cacgaaatc gccaaattt gtccttcca gacgtcgtc gaaagcact ccttgctac cacaataac tacctatac gtgacttgc tttgatggc aatttatac tgtcttgat ttcgcatca aattcaatc aaatgggct tataagaca aatgacagg gtcaagtgc accccaatt ttgtgctcc agtggcgca agaatcatt ttcgggtct tacgataag cacatccat tgcgtcgat attatgcaa ggaggcgtt gtttggacc tcgtttgtt tccgaaagt gtgatttca aaacctcta cttttaaac caaaacatt tacgtggcc acgatttgt ggcacttgt ctctgcctt tcggaaact tccggtgct attttatgg caatacaaa accggaagt ccgattttt ggggctccg tgcacccac aacgggtgc attttatgg ccaaatgta accggaagc gtcatatgt atggcttta gatggccgc aaattatgg cagtttgat acagggggc catttatac tcgtctttc gccagacga gacaatttt ctattttta gggtcacat gatcgtaat ttgtacgtg ttaaaaatt gaaagtgaa gcccctgag ccccaattc gtgcttaac ttagatgat tgtttaaca actccgttc atttcgggc gattttata attgtggcg gggaattcg ggtcgtttg tttgtggtg acgtttagg ggtgagttg ggggctcaa ttggggttg ccgggggag gttttttcg tcggttgtg ggcggtgac ggacgattt tttgtaggg tgtcgtgat aataacttg tattgtgtt gaaattaaa aacagttga
- >TC008000 RA
aaaacacaa aaaattgca aagacacag accaaaaaa ttttgcaaa tgatttgtg cctaacttc cctccgctg acataaccc aagtttttc aaaaatggg ggctccaga aaaccgagc gataatttt gagtgactg tggcaccgt tctaagtgc tcttagtta caaagacgt caattcatt acgtagttc gataaccca gttcatatc ggtgcatgt cacagaaaa acaccaatg cataggtct tgcaatgaa tcacaatat ttacattcc ttcgattat tttaagttt acaggaatt gcaccactg ttttatttt tttgtgtga cgaaactga gtcaattac aacaaattt ggggcttct ctggtcatt atctttcgc ttcgggcaa taacaccgt cgccacttt attactaaa tggccaaaa actcgtctt gaacaaaca caaagtgac tcacgtaac gaattatcc gaatatttt ttacataat tcgaacatc aggcagtac aggtgtgag caagtttgt gagggttac tcactcaag tattgaacg caacgtcaa acatttgag taaaatatt caaaatcgg ggcttccga tacgattta cttcggcac cccgctcac ttttgaccc ctcaatgat cgagttgtt gctcgccct cgaaaatgg ggcttgtct cttaataac gccccctaa gaaccaaat caatccggt gcgtctcca ccagacgtt gttccatcc ccaaaatgg ggccactgt cctccaaat cgtcccgtc tctatttct acgatggtc ggagacaca aatcagtga ttttctcca aaaatcaaa attaaaaat cctagcttt tgggggcga accgttccc tttatctct tctcgctta tcaaaagtc ggatttttt gcgaatttt taatttgta cggagtcac tgaagtgtc atgttgggc cactgtttg cgaagtcgc ggaaggttt aggggaagt accgctcgg ggaggaatt agacgacac tattgtgga gttaaggga tgatttcgg acgggttgt ggaaaatgg tgaaggaga aattttcat tgggagttc gacccgaat ttgcttcgt gggtgatga gaaaattca tcacaaaac accagtttt tcgggctac tggtgacat cgggtttat ttctgataa gaagattta ttacaaagg gcgcaaaaa cgatgtttt taaacgctt gggccataa agtacaatt aagtaaaat tgaaacaat ggtattgga cgagacagg gttacggaa cgtttgtgt gtggtctaa aacccgcac aaagctttt acttttttt gatagtcga aaactgcga taacagtac caaaagcaa ggttttaga taaggtcag gattaaact tttacacac tttaccgaa acaatactt ccctgattt tatcgatat cattcgaaa gtgcccaat cacttcgca tggaaaaac cgataagaa ctctctcga atcgattta tttgaatcg aaatagaac cgataagag acaagttga tatttttaa aacactgat ttgtaaata ttttggtgt tcgaaattt tgataaaaa cacattttt cgaacttgg gggcaactc gattcttgc agtgcaatt tttgagcga attcgagga cgaaacagg ggcagcttg tcccgattt agtgactca tttgtttga aagtgactt aaacacctg tattgatat tttacgtga cgttaaagt cgtacaaaa aagaccaca aactgaacc gcccccgaa aaatgagaa aaaaataaa aaatagcaa catcggaat tgaattaaa aattgcttg gagctataa cttggaggc ttgcgtcga ctcaacacc tctcgtttt ttctcacaa tggacgcga gttagtcgc cgttggggc gttttcgca catttttgc cattttaga cacgcaaag tggccacga aatcgccaa atttgtcct tccagacgt cgtcgaaag cactccttg ctaccacaa taactacct atacgtgac ttgctttga tggcaattt atactgtct tgatttcgc atcaaattc aatcaaatg ggcttataa gacaaatga cagggtcaa gtgcacccc aattttgtg ctccagtgg cgcaagaat cattttcgg gtcttacga taagcacat ccattgcgt cgatattat gcaaggagg cgttgtttg gacctcgtt tgtttccga aagtgtgat ttcaaaacc tctactttt aaaccaaaa catttacgt ggccacgat ttgtggcac ttgtctctg cctttcgga aacttccgg tgctatttt atggcaata caaaaccgg aagtccgat ttttggggc tccgtgcac ccacaacgg gtgcatttt atggccaaa tgtaaccgg aagcgtcat atgtatggc tttagatgg ccgcaaatt atggcagtt tgatacagg gggccattt atactcgtc tttcgccag acgagacaa ttttctatt tttagggtc acatgatcg taatttgta cgtgttaaa aattgaaag tgaagcccc tgagcccca attcgtgct taacttaga tgattgttt aacaactcc gttcatttc gggcgattt tataattgt ggcggggaa ttcgggtcg tttgtttgt ggtgacgtt taggggtga gttgggggc tcaattggg gttgccggg ggaggtttt ttcgtcggt tgtgggcgg tgacggacg attttttgt agggtgtcg tgataataa cttgtattg tgttgaaat taaaaacag ttgacttga acttgattg tttccgatt tttgttggt ttgttaatt aaacaatgt acgaaaaa
