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Gene information
General information
TC007942 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2986352..2990932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007942
    iBeetle screen iB_06245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 12.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007942 RA
      MSEFKLQHL VAELLNCLG AKNPPEKLV EALQKSISS 
STGKECSGS QVAIQSYVQ RIAEDLKNS EPFLLKFED 
LKQKNVDCL GPYVQLLYH ISQDSSVRS LLGKMDKHS 
EQKTEITRD DLPQVRNRL RREALKRSQ KECSSSSIL 
NTSTNLPRA STPSVTSWV QRRPTLSCD FSTQSCSTI 
CPAVPVVSQ ENILIKDLL VVLIGIPGC YIEAEELND 
PYAPRTFKI NDNVPLPLQ ELVKQILPI ASHYSLIQR 
FAEEKLRFE FGQVNNALA EEMSAILKE HLIYVTQLQ 
DEFEADNLN LQKLWFYVQ KRLQPFGIT ANIATKISK 
SDAKGGKVL SLLHDHIIG SIGDERTQQ RCVSLMEAA 
CVPYMKMLG MWIYRGIIS DPISEFLVK DNEVIQKED 
MPVDYSADY WDKKYSIER EKIPKFLEP VSDIILKAG 
KYLNVIRQC GKPVKNKIR PILYKIEEK HYIEAIENA 
YKFASQTLL ELVVKEKDL LGRLRSVKH YFLLDQGDF 
IVTFLTLCE KELSKDVGD VIQGRIESL MDLALRLSS 
AVSDPYKDD LRTELLPYD LQFQMMKIL TIQTNMEQD 
FRTAYERQK LLVIDSFSF SYEVHWPLS LILNRKSLA 
CYQMIFRHL FYCKYVERM ICQVWKCNK VAKKFHFDD 
AQHYRAAFA LRQRMLHCV QNLEYHMMV EVIEPHWCL 
FLQKISKVN NVDEILFCH RDFLDSCLK DCMLTIPPI 
LRTVTKLLA ICVDFCQFM QNEKAFQDS ASHASTAPS 
FKERISLFD VQFSVALVN LLDQINNLN KDSSEHDRL 
FNLLYRLDF NSFYSGDLM KKDFGTITI ESSG
    CDS sequence
    • >TC007942 RA
      atgagcgaa tttaagctc cagcacttg gttgcggaa 
cttttgaac tgtttgggt gcaaaaaat ccccccgaa 
aaacttgta gaagcgtta caaaaaagt atcagcagt 
agcaccggc aaggaatgt tcaggttca caagtggca 
atacagagt tacgtgcaa cgtattgcc gaagatttg 
aaaaattcg gaaccattt ctactgaaa tttgaagat 
ctaaagcag aaaaatgtg gattgttta gggccgtac 
gttcagctt ctgtatcac atatctcaa gatagtagt 
gttagaagc ttgttaggt aaaatggat aagcattcg 
gagcaaaaa accgaaata acgcgggac gatttgcca 
caagttagg aatcggtta cgtcgggag gctttaaaa 
cggtcgcaa aaagagtgt tcttcaagc agtattctc 
aacactagt actaatctt ccgagggct agcacccct 
tctgttacg tcttgggtc cagagacgg ccgacttta 
tcctgcgat ttttcaacg caaagttgt agtactatt 
tgtccagct gttccagtt gtgtctcaa gaaaacatc 
ctgattaaa gatctgtta gttgtatta attggtata 
ccaggctgt tacatcgaa gcagaggag ctgaatgat 
ccttacgcc cctagaacc tttaaaata aacgataat 
gtacctctg cctctgcaa gaacttgtt aaacaaatc 
ttgcctatc gccagtcat tattcgcta atccagagg 
tttgccgaa gaaaagttg aggtttgag tttgggcaa 
gttaataac gccttagct gaagaaatg agcgcgatt 
ttgaaagaa catctaatt tatgtcacc caacttcag 
gatgaattt gaagctgat aacttaaat ttgcaaaaa 
ctttggttt tatgtacag aagcggttg cagcctttt 
ggcattaca gcaaacatt gcgactaag atcagcaaa 
tcggatgcc aaaggggga aaagtttta agtttgcta 
catgatcac ataattggg tcaattggt gatgaaagg 
acacaacaa cgttgtgtt agtctgatg gaggctgcg 
tgtgttccg tatatgaaa atgttggga atgtggatt 
tacagagga attatttct gatccaatc agtgagttt 
ctggtaaaa gacaatgaa gtcatccag aaggaagac 
atgccggtc gattattca gccgactac tgggataaa 
aaatactca atagaacgg gaaaagatc ccgaaattt 
ttagagcca gtctcggac ataattcta aaagctgga 
aaatacttg aacgtaata cgacagtgt ggcaagcct 
gtgaaaaat aaaatacga ccaatactt tacaaaatc 
gaagaaaag cactacatt gaagcgatc gaaaatgcg 
tataaattt gcaagccaa acattactc gagctagtt 
gtgaaggaa aaggatctt ttggggaga ctgagatcc 
gtcaaacac tattttctc ttagaccag ggcgatttt 
atcgtaacg tttctcact ttgtgtgaa aaagaactg 
agtaaagat gttggcgat gtgattcaa gggcgcatc 
gaatcgctg atggatctg gctttgagg ctgtcaagc 
gcagtgagc gacccatac aaggacgat ttaaggacc 
gagctcttg ccctatgat ctgcaattt caaatgatg 
aaaatactc acaattcag acaaatatg gagcaagac 
tttcggacc gcctacgag cgccagaaa cttctcgtt 
attgactcg ttctcgttc agttacgaa gtgcactgg 
cccttgtct ttgattctg aacagaaaa tctctggcg 
tgttaccag atgattttt aggcatttg ttctattgc 
aagtatgtg gagagaatg atttgccaa gtttggaag 
tgcaataaa gttgcgaaa aagttccat tttgacgat 
gcgcagcac tatagggcc gcctttgct ctgcgacag 
aggatgtta cattgcgtt cagaatttg gaatatcat 
atgatggtg gaggtgatt gaacctcac tggtgctta 
tttctgcaa aaaatttcc aaagtcaac aacgtcgat 
gaaatattg ttttgccat cgcgatttt ttggatagc 
tgtctgaag gactgcatg ctcacaatt cctccgatt 
cttcgcacg gttaccaag cttttggca atttgcgtg 
gatttctgt caatttatg cagaatgag aaagccttc 
caagacagt gcaagccac gcctccaca gcgccaagc 
ttcaaggaa agaatttct ctattcgac gtacaattt 
tcagtcgct ttggtaaac ttgctggat cagattaat 
aatttaaac aaggatagc agtgaacac gacaggctc 
tttaatttg ttatacagg ctggacttc aattcgttt 
tattctggc gatttaatg aagaaggat tttgggacc 
atcacaatt gaatcgtct ggttga
    mRNA sequence
    • >TC007942 RA
      tcagttttt caaccttta ttgatttga aacgcctgt 
taggttaat tgtttttga caagtatta tcggcaaaa 
tgagcgaat ttaagctcc agcacttgg ttgcggaac 
ttttgaact gtttgggtg caaaaaatc cccccgaaa 
aacttgtag aagcgttac aaaaaagta tcagcagta 
gcaccggca aggaatgtt caggttcac aagtggcaa 
tacagagtt acgtgcaac gtattgccg aagatttga 
aaaattcgg aaccatttc tactgaaat ttgaagatc 
taaagcaga aaaatgtgg attgtttag ggccgtacg 
ttcagcttc tgtatcaca tatctcaag atagtagtg 
ttagaagct tgttaggta aaatggata agcattcgg 
agcaaaaaa ccgaaataa cgcgggacg atttgccac 
aagttagga atcggttac gtcgggagg ctttaaaac 
ggtcgcaaa aagagtgtt cttcaagca gtattctca 
acactagta ctaatcttc cgagggcta gcacccctt 
ctgttacgt cttgggtcc agagacggc cgactttat 
cctgcgatt tttcaacgc aaagttgta gtactattt 
gtccagctg ttccagttg tgtctcaag aaaacatcc 
tgattaaag atctgttag ttgtattaa ttggtatac 
caggctgtt acatcgaag cagaggagc tgaatgatc 
cttacgccc ctagaacct ttaaaataa acgataatg 
tacctctgc ctctgcaag aacttgtta aacaaatct 
tgcctatcg ccagtcatt attcgctaa tccagaggt 
ttgccgaag aaaagttga ggtttgagt ttgggcaag 
ttaataacg ccttagctg aagaaatga gcgcgattt 
tgaaagaac atctaattt atgtcaccc aacttcagg 
atgaatttg aagctgata acttaaatt tgcaaaaac 
tttggtttt atgtacaga agcggttgc agccttttg 
gcattacag caaacattg cgactaaga tcagcaaat 
cggatgcca aagggggaa aagttttaa gtttgctac 
atgatcaca taattgggt caattggtg atgaaagga 
cacaacaac gttgtgtta gtctgatgg aggctgcgt 
gtgttccgt atatgaaaa tgttgggaa tgtggattt 
acagaggaa ttatttctg atccaatca gtgagtttc 
tggtaaaag acaatgaag tcatccaga aggaagaca 
tgccggtcg attattcag ccgactact gggataaaa 
aatactcaa tagaacggg aaaagatcc cgaaatttt 
tagagccag tctcggaca taattctaa aagctggaa 
aatacttga acgtaatac gacagtgtg gcaagcctg 
tgaaaaata aaatacgac caatacttt acaaaatcg 
aagaaaagc actacattg aagcgatcg aaaatgcgt 
ataaatttg caagccaaa cattactcg agctagttg 
tgaaggaaa aggatcttt tggggagac tgagatccg 
tcaaacact attttctct tagaccagg gcgatttta 
tcgtaacgt ttctcactt tgtgtgaaa aagaactga 
gtaaagatg ttggcgatg tgattcaag ggcgcatcg 
aatcgctga tggatctgg ctttgaggc tgtcaagcg 
cagtgagcg acccataca aggacgatt taaggaccg 
agctcttgc cctatgatc tgcaatttc aaatgatga 
aaatactca caattcaga caaatatgg agcaagact 
ttcggaccg cctacgagc gccagaaac ttctcgtta 
ttgactcgt tctcgttca gttacgaag tgcactggc 
ccttgtctt tgattctga acagaaaat ctctggcgt 
gttaccaga tgattttta ggcatttgt tctattgca 
agtatgtgg agagaatga tttgccaag tttggaagt 
gcaataaag ttgcgaaaa agttccatt ttgacgatg 
cgcagcact atagggccg cctttgctc tgcgacaga 
ggatgttac attgcgttc agaatttgg aatatcata 
tgatggtgg aggtgattg aacctcact ggtgcttat 
ttctgcaaa aaatttcca aagtcaaca acgtcgatg 
aaatattgt tttgccatc gcgattttt tggatagct 
gtctgaagg actgcatgc tcacaattc ctccgattc 
ttcgcacgg ttaccaagc ttttggcaa tttgcgtgg 
atttctgtc aatttatgc agaatgaga aagccttcc 
aagacagtg caagccacg cctccacag cgccaagct 
tcaaggaaa gaatttctc tattcgacg tacaatttt 
cagtcgctt tggtaaact tgctggatc agattaata 
atttaaaca aggatagca gtgaacacg acaggctct 
ttaatttgt tatacaggc tggacttca attcgtttt 
attctggcg atttaatga agaaggatt ttgggacca 
tcacaattg aatcgtctg gttgattag cttagatgt 
acaattttt tggtgtaat tttgattta catctataa 
gttagggtt tatgtagaa tatttatgt tagcatagt 
taattaata aaaaatcaa aaccgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY