| General information | |||
| TC007942 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 2986352..2990932 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007942 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06245 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 12.5% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
- >TC007942 RA
MSEFKLQHL VAELLNCLG AKNPPEKLV EALQKSISS STGKECSGS QVAIQSYVQ RIAEDLKNS EPFLLKFED LKQKNVDCL GPYVQLLYH ISQDSSVRS LLGKMDKHS EQKTEITRD DLPQVRNRL RREALKRSQ KECSSSSIL NTSTNLPRA STPSVTSWV QRRPTLSCD FSTQSCSTI CPAVPVVSQ ENILIKDLL VVLIGIPGC YIEAEELND PYAPRTFKI NDNVPLPLQ ELVKQILPI ASHYSLIQR FAEEKLRFE FGQVNNALA EEMSAILKE HLIYVTQLQ DEFEADNLN LQKLWFYVQ KRLQPFGIT ANIATKISK SDAKGGKVL SLLHDHIIG SIGDERTQQ RCVSLMEAA CVPYMKMLG MWIYRGIIS DPISEFLVK DNEVIQKED MPVDYSADY WDKKYSIER EKIPKFLEP VSDIILKAG KYLNVIRQC GKPVKNKIR PILYKIEEK HYIEAIENA YKFASQTLL ELVVKEKDL LGRLRSVKH YFLLDQGDF IVTFLTLCE KELSKDVGD VIQGRIESL MDLALRLSS AVSDPYKDD LRTELLPYD LQFQMMKIL TIQTNMEQD FRTAYERQK LLVIDSFSF SYEVHWPLS LILNRKSLA CYQMIFRHL FYCKYVERM ICQVWKCNK VAKKFHFDD AQHYRAAFA LRQRMLHCV QNLEYHMMV EVIEPHWCL FLQKISKVN NVDEILFCH RDFLDSCLK DCMLTIPPI LRTVTKLLA ICVDFCQFM QNEKAFQDS ASHASTAPS FKERISLFD VQFSVALVN LLDQINNLN KDSSEHDRL FNLLYRLDF NSFYSGDLM KKDFGTITI ESSG
- >TC007942 RA
atgagcgaa tttaagctc cagcacttg gttgcggaa cttttgaac tgtttgggt gcaaaaaat ccccccgaa aaacttgta gaagcgtta caaaaaagt atcagcagt agcaccggc aaggaatgt tcaggttca caagtggca atacagagt tacgtgcaa cgtattgcc gaagatttg aaaaattcg gaaccattt ctactgaaa tttgaagat ctaaagcag aaaaatgtg gattgttta gggccgtac gttcagctt ctgtatcac atatctcaa gatagtagt gttagaagc ttgttaggt aaaatggat aagcattcg gagcaaaaa accgaaata acgcgggac gatttgcca caagttagg aatcggtta cgtcgggag gctttaaaa cggtcgcaa aaagagtgt tcttcaagc agtattctc aacactagt actaatctt ccgagggct agcacccct tctgttacg tcttgggtc cagagacgg ccgacttta tcctgcgat ttttcaacg caaagttgt agtactatt tgtccagct gttccagtt gtgtctcaa gaaaacatc ctgattaaa gatctgtta gttgtatta attggtata ccaggctgt tacatcgaa gcagaggag ctgaatgat ccttacgcc cctagaacc tttaaaata aacgataat gtacctctg cctctgcaa gaacttgtt aaacaaatc ttgcctatc gccagtcat tattcgcta atccagagg tttgccgaa gaaaagttg aggtttgag tttgggcaa gttaataac gccttagct gaagaaatg agcgcgatt ttgaaagaa catctaatt tatgtcacc caacttcag gatgaattt gaagctgat aacttaaat ttgcaaaaa ctttggttt tatgtacag aagcggttg cagcctttt ggcattaca gcaaacatt gcgactaag atcagcaaa tcggatgcc aaaggggga aaagtttta agtttgcta catgatcac ataattggg tcaattggt gatgaaagg acacaacaa cgttgtgtt agtctgatg gaggctgcg tgtgttccg tatatgaaa atgttggga atgtggatt tacagagga attatttct gatccaatc agtgagttt ctggtaaaa gacaatgaa gtcatccag aaggaagac atgccggtc gattattca gccgactac tgggataaa aaatactca atagaacgg gaaaagatc ccgaaattt ttagagcca gtctcggac ataattcta aaagctgga aaatacttg aacgtaata cgacagtgt ggcaagcct gtgaaaaat aaaatacga ccaatactt tacaaaatc gaagaaaag cactacatt gaagcgatc gaaaatgcg tataaattt gcaagccaa acattactc gagctagtt gtgaaggaa aaggatctt ttggggaga ctgagatcc gtcaaacac tattttctc ttagaccag ggcgatttt atcgtaacg tttctcact ttgtgtgaa aaagaactg agtaaagat gttggcgat gtgattcaa gggcgcatc gaatcgctg atggatctg gctttgagg ctgtcaagc gcagtgagc gacccatac aaggacgat ttaaggacc gagctcttg ccctatgat ctgcaattt caaatgatg aaaatactc acaattcag acaaatatg gagcaagac tttcggacc gcctacgag cgccagaaa cttctcgtt attgactcg ttctcgttc agttacgaa gtgcactgg cccttgtct ttgattctg aacagaaaa tctctggcg tgttaccag atgattttt aggcatttg ttctattgc aagtatgtg gagagaatg atttgccaa gtttggaag tgcaataaa gttgcgaaa aagttccat tttgacgat gcgcagcac tatagggcc gcctttgct ctgcgacag aggatgtta cattgcgtt cagaatttg gaatatcat atgatggtg gaggtgatt gaacctcac tggtgctta tttctgcaa aaaatttcc aaagtcaac aacgtcgat gaaatattg ttttgccat cgcgatttt ttggatagc tgtctgaag gactgcatg ctcacaatt cctccgatt cttcgcacg gttaccaag cttttggca atttgcgtg gatttctgt caatttatg cagaatgag aaagccttc caagacagt gcaagccac gcctccaca gcgccaagc ttcaaggaa agaatttct ctattcgac gtacaattt tcagtcgct ttggtaaac ttgctggat cagattaat aatttaaac aaggatagc agtgaacac gacaggctc tttaatttg ttatacagg ctggacttc aattcgttt tattctggc gatttaatg aagaaggat tttgggacc atcacaatt gaatcgtct ggttga
- >TC007942 RA
tcagttttt caaccttta ttgatttga aacgcctgt taggttaat tgtttttga caagtatta tcggcaaaa tgagcgaat ttaagctcc agcacttgg ttgcggaac ttttgaact gtttgggtg caaaaaatc cccccgaaa aacttgtag aagcgttac aaaaaagta tcagcagta gcaccggca aggaatgtt caggttcac aagtggcaa tacagagtt acgtgcaac gtattgccg aagatttga aaaattcgg aaccatttc tactgaaat ttgaagatc taaagcaga aaaatgtgg attgtttag ggccgtacg ttcagcttc tgtatcaca tatctcaag atagtagtg ttagaagct tgttaggta aaatggata agcattcgg agcaaaaaa ccgaaataa cgcgggacg atttgccac aagttagga atcggttac gtcgggagg ctttaaaac ggtcgcaaa aagagtgtt cttcaagca gtattctca acactagta ctaatcttc cgagggcta gcacccctt ctgttacgt cttgggtcc agagacggc cgactttat cctgcgatt tttcaacgc aaagttgta gtactattt gtccagctg ttccagttg tgtctcaag aaaacatcc tgattaaag atctgttag ttgtattaa ttggtatac caggctgtt acatcgaag cagaggagc tgaatgatc cttacgccc ctagaacct ttaaaataa acgataatg tacctctgc ctctgcaag aacttgtta aacaaatct tgcctatcg ccagtcatt attcgctaa tccagaggt ttgccgaag aaaagttga ggtttgagt ttgggcaag ttaataacg ccttagctg aagaaatga gcgcgattt tgaaagaac atctaattt atgtcaccc aacttcagg atgaatttg aagctgata acttaaatt tgcaaaaac tttggtttt atgtacaga agcggttgc agccttttg gcattacag caaacattg cgactaaga tcagcaaat cggatgcca aagggggaa aagttttaa gtttgctac atgatcaca taattgggt caattggtg atgaaagga cacaacaac gttgtgtta gtctgatgg aggctgcgt gtgttccgt atatgaaaa tgttgggaa tgtggattt acagaggaa ttatttctg atccaatca gtgagtttc tggtaaaag acaatgaag tcatccaga aggaagaca tgccggtcg attattcag ccgactact gggataaaa aatactcaa tagaacggg aaaagatcc cgaaatttt tagagccag tctcggaca taattctaa aagctggaa aatacttga acgtaatac gacagtgtg gcaagcctg tgaaaaata aaatacgac caatacttt acaaaatcg aagaaaagc actacattg aagcgatcg aaaatgcgt ataaatttg caagccaaa cattactcg agctagttg tgaaggaaa aggatcttt tggggagac tgagatccg tcaaacact attttctct tagaccagg gcgatttta tcgtaacgt ttctcactt tgtgtgaaa aagaactga gtaaagatg ttggcgatg tgattcaag ggcgcatcg aatcgctga tggatctgg ctttgaggc tgtcaagcg cagtgagcg acccataca aggacgatt taaggaccg agctcttgc cctatgatc tgcaatttc aaatgatga aaatactca caattcaga caaatatgg agcaagact ttcggaccg cctacgagc gccagaaac ttctcgtta ttgactcgt tctcgttca gttacgaag tgcactggc ccttgtctt tgattctga acagaaaat ctctggcgt gttaccaga tgattttta ggcatttgt tctattgca agtatgtgg agagaatga tttgccaag tttggaagt gcaataaag ttgcgaaaa agttccatt ttgacgatg cgcagcact atagggccg cctttgctc tgcgacaga ggatgttac attgcgttc agaatttgg aatatcata tgatggtgg aggtgattg aacctcact ggtgcttat ttctgcaaa aaatttcca aagtcaaca acgtcgatg aaatattgt tttgccatc gcgattttt tggatagct gtctgaagg actgcatgc tcacaattc ctccgattc ttcgcacgg ttaccaagc ttttggcaa tttgcgtgg atttctgtc aatttatgc agaatgaga aagccttcc aagacagtg caagccacg cctccacag cgccaagct tcaaggaaa gaatttctc tattcgacg tacaatttt cagtcgctt tggtaaact tgctggatc agattaata atttaaaca aggatagca gtgaacacg acaggctct ttaatttgt tatacaggc tggacttca attcgtttt attctggcg atttaatga agaaggatt ttgggacca tcacaattg aatcgtctg gttgattag cttagatgt acaattttt tggtgtaat tttgattta catctataa gttagggtt tatgtagaa tatttatgt tagcatagt taattaata aaaaatcaa aaccgca
