Status
Please wait ...
Gene information
General information
TC007942 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2986352..2990932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007942
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 12.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007942 RA
      MSEFKLQHL VAELLNCLG AKNPPEKLV EALQKSISS 
      STGKECSGS QVAIQSYVQ RIAEDLKNS EPFLLKFED 
      LKQKNVDCL GPYVQLLYH ISQDSSVRS LLGKMDKHS 
      EQKTEITRD DLPQVRNRL RREALKRSQ KECSSSSIL 
      NTSTNLPRA STPSVTSWV QRRPTLSCD FSTQSCSTI 
      CPAVPVVSQ ENILIKDLL VVLIGIPGC YIEAEELND 
      PYAPRTFKI NDNVPLPLQ ELVKQILPI ASHYSLIQR 
      FAEEKLRFE FGQVNNALA EEMSAILKE HLIYVTQLQ 
      DEFEADNLN LQKLWFYVQ KRLQPFGIT ANIATKISK 
      SDAKGGKVL SLLHDHIIG SIGDERTQQ RCVSLMEAA 
      CVPYMKMLG MWIYRGIIS DPISEFLVK DNEVIQKED 
      MPVDYSADY WDKKYSIER EKIPKFLEP VSDIILKAG 
      KYLNVIRQC GKPVKNKIR PILYKIEEK HYIEAIENA 
      YKFASQTLL ELVVKEKDL LGRLRSVKH YFLLDQGDF 
      IVTFLTLCE KELSKDVGD VIQGRIESL MDLALRLSS 
      AVSDPYKDD LRTELLPYD LQFQMMKIL TIQTNMEQD 
      FRTAYERQK LLVIDSFSF SYEVHWPLS LILNRKSLA 
      CYQMIFRHL FYCKYVERM ICQVWKCNK VAKKFHFDD 
      AQHYRAAFA LRQRMLHCV QNLEYHMMV EVIEPHWCL 
      FLQKISKVN NVDEILFCH RDFLDSCLK DCMLTIPPI 
      LRTVTKLLA ICVDFCQFM QNEKAFQDS ASHASTAPS 
      FKERISLFD VQFSVALVN LLDQINNLN KDSSEHDRL 
      FNLLYRLDF NSFYSGDLM KKDFGTITI ESSG
    CDS sequence
    • >TC007942 RA
      atgagcgaa tttaagctc cagcacttg gttgcggaa 
      cttttgaac tgtttgggt gcaaaaaat ccccccgaa 
      aaacttgta gaagcgtta caaaaaagt atcagcagt 
      agcaccggc aaggaatgt tcaggttca caagtggca 
      atacagagt tacgtgcaa cgtattgcc gaagatttg 
      aaaaattcg gaaccattt ctactgaaa tttgaagat 
      ctaaagcag aaaaatgtg gattgttta gggccgtac 
      gttcagctt ctgtatcac atatctcaa gatagtagt 
      gttagaagc ttgttaggt aaaatggat aagcattcg 
      gagcaaaaa accgaaata acgcgggac gatttgcca 
      caagttagg aatcggtta cgtcgggag gctttaaaa 
      cggtcgcaa aaagagtgt tcttcaagc agtattctc 
      aacactagt actaatctt ccgagggct agcacccct 
      tctgttacg tcttgggtc cagagacgg ccgacttta 
      tcctgcgat ttttcaacg caaagttgt agtactatt 
      tgtccagct gttccagtt gtgtctcaa gaaaacatc 
      ctgattaaa gatctgtta gttgtatta attggtata 
      ccaggctgt tacatcgaa gcagaggag ctgaatgat 
      ccttacgcc cctagaacc tttaaaata aacgataat 
      gtacctctg cctctgcaa gaacttgtt aaacaaatc 
      ttgcctatc gccagtcat tattcgcta atccagagg 
      tttgccgaa gaaaagttg aggtttgag tttgggcaa 
      gttaataac gccttagct gaagaaatg agcgcgatt 
      ttgaaagaa catctaatt tatgtcacc caacttcag 
      gatgaattt gaagctgat aacttaaat ttgcaaaaa 
      ctttggttt tatgtacag aagcggttg cagcctttt 
      ggcattaca gcaaacatt gcgactaag atcagcaaa 
      tcggatgcc aaaggggga aaagtttta agtttgcta 
      catgatcac ataattggg tcaattggt gatgaaagg 
      acacaacaa cgttgtgtt agtctgatg gaggctgcg 
      tgtgttccg tatatgaaa atgttggga atgtggatt 
      tacagagga attatttct gatccaatc agtgagttt 
      ctggtaaaa gacaatgaa gtcatccag aaggaagac 
      atgccggtc gattattca gccgactac tgggataaa 
      aaatactca atagaacgg gaaaagatc ccgaaattt 
      ttagagcca gtctcggac ataattcta aaagctgga 
      aaatacttg aacgtaata cgacagtgt ggcaagcct 
      gtgaaaaat aaaatacga ccaatactt tacaaaatc 
      gaagaaaag cactacatt gaagcgatc gaaaatgcg 
      tataaattt gcaagccaa acattactc gagctagtt 
      gtgaaggaa aaggatctt ttggggaga ctgagatcc 
      gtcaaacac tattttctc ttagaccag ggcgatttt 
      atcgtaacg tttctcact ttgtgtgaa aaagaactg 
      agtaaagat gttggcgat gtgattcaa gggcgcatc 
      gaatcgctg atggatctg gctttgagg ctgtcaagc 
      gcagtgagc gacccatac aaggacgat ttaaggacc 
      gagctcttg ccctatgat ctgcaattt caaatgatg 
      aaaatactc acaattcag acaaatatg gagcaagac 
      tttcggacc gcctacgag cgccagaaa cttctcgtt 
      attgactcg ttctcgttc agttacgaa gtgcactgg 
      cccttgtct ttgattctg aacagaaaa tctctggcg 
      tgttaccag atgattttt aggcatttg ttctattgc 
      aagtatgtg gagagaatg atttgccaa gtttggaag 
      tgcaataaa gttgcgaaa aagttccat tttgacgat 
      gcgcagcac tatagggcc gcctttgct ctgcgacag 
      aggatgtta cattgcgtt cagaatttg gaatatcat 
      atgatggtg gaggtgatt gaacctcac tggtgctta 
      tttctgcaa aaaatttcc aaagtcaac aacgtcgat 
      gaaatattg ttttgccat cgcgatttt ttggatagc 
      tgtctgaag gactgcatg ctcacaatt cctccgatt 
      cttcgcacg gttaccaag cttttggca atttgcgtg 
      gatttctgt caatttatg cagaatgag aaagccttc 
      caagacagt gcaagccac gcctccaca gcgccaagc 
      ttcaaggaa agaatttct ctattcgac gtacaattt 
      tcagtcgct ttggtaaac ttgctggat cagattaat 
      aatttaaac aaggatagc agtgaacac gacaggctc 
      tttaatttg ttatacagg ctggacttc aattcgttt 
      tattctggc gatttaatg aagaaggat tttgggacc 
      atcacaatt gaatcgtct ggttga
    mRNA sequence
    • >TC007942 RA
      tcagttttt caaccttta ttgatttga aacgcctgt 
      taggttaat tgtttttga caagtatta tcggcaaaa 
      tgagcgaat ttaagctcc agcacttgg ttgcggaac 
      ttttgaact gtttgggtg caaaaaatc cccccgaaa 
      aacttgtag aagcgttac aaaaaagta tcagcagta 
      gcaccggca aggaatgtt caggttcac aagtggcaa 
      tacagagtt acgtgcaac gtattgccg aagatttga 
      aaaattcgg aaccatttc tactgaaat ttgaagatc 
      taaagcaga aaaatgtgg attgtttag ggccgtacg 
      ttcagcttc tgtatcaca tatctcaag atagtagtg 
      ttagaagct tgttaggta aaatggata agcattcgg 
      agcaaaaaa ccgaaataa cgcgggacg atttgccac 
      aagttagga atcggttac gtcgggagg ctttaaaac 
      ggtcgcaaa aagagtgtt cttcaagca gtattctca 
      acactagta ctaatcttc cgagggcta gcacccctt 
      ctgttacgt cttgggtcc agagacggc cgactttat 
      cctgcgatt tttcaacgc aaagttgta gtactattt 
      gtccagctg ttccagttg tgtctcaag aaaacatcc 
      tgattaaag atctgttag ttgtattaa ttggtatac 
      caggctgtt acatcgaag cagaggagc tgaatgatc 
      cttacgccc ctagaacct ttaaaataa acgataatg 
      tacctctgc ctctgcaag aacttgtta aacaaatct 
      tgcctatcg ccagtcatt attcgctaa tccagaggt 
      ttgccgaag aaaagttga ggtttgagt ttgggcaag 
      ttaataacg ccttagctg aagaaatga gcgcgattt 
      tgaaagaac atctaattt atgtcaccc aacttcagg 
      atgaatttg aagctgata acttaaatt tgcaaaaac 
      tttggtttt atgtacaga agcggttgc agccttttg 
      gcattacag caaacattg cgactaaga tcagcaaat 
      cggatgcca aagggggaa aagttttaa gtttgctac 
      atgatcaca taattgggt caattggtg atgaaagga 
      cacaacaac gttgtgtta gtctgatgg aggctgcgt 
      gtgttccgt atatgaaaa tgttgggaa tgtggattt 
      acagaggaa ttatttctg atccaatca gtgagtttc 
      tggtaaaag acaatgaag tcatccaga aggaagaca 
      tgccggtcg attattcag ccgactact gggataaaa 
      aatactcaa tagaacggg aaaagatcc cgaaatttt 
      tagagccag tctcggaca taattctaa aagctggaa 
      aatacttga acgtaatac gacagtgtg gcaagcctg 
      tgaaaaata aaatacgac caatacttt acaaaatcg 
      aagaaaagc actacattg aagcgatcg aaaatgcgt 
      ataaatttg caagccaaa cattactcg agctagttg 
      tgaaggaaa aggatcttt tggggagac tgagatccg 
      tcaaacact attttctct tagaccagg gcgatttta 
      tcgtaacgt ttctcactt tgtgtgaaa aagaactga 
      gtaaagatg ttggcgatg tgattcaag ggcgcatcg 
      aatcgctga tggatctgg ctttgaggc tgtcaagcg 
      cagtgagcg acccataca aggacgatt taaggaccg 
      agctcttgc cctatgatc tgcaatttc aaatgatga 
      aaatactca caattcaga caaatatgg agcaagact 
      ttcggaccg cctacgagc gccagaaac ttctcgtta 
      ttgactcgt tctcgttca gttacgaag tgcactggc 
      ccttgtctt tgattctga acagaaaat ctctggcgt 
      gttaccaga tgattttta ggcatttgt tctattgca 
      agtatgtgg agagaatga tttgccaag tttggaagt 
      gcaataaag ttgcgaaaa agttccatt ttgacgatg 
      cgcagcact atagggccg cctttgctc tgcgacaga 
      ggatgttac attgcgttc agaatttgg aatatcata 
      tgatggtgg aggtgattg aacctcact ggtgcttat 
      ttctgcaaa aaatttcca aagtcaaca acgtcgatg 
      aaatattgt tttgccatc gcgattttt tggatagct 
      gtctgaagg actgcatgc tcacaattc ctccgattc 
      ttcgcacgg ttaccaagc ttttggcaa tttgcgtgg 
      atttctgtc aatttatgc agaatgaga aagccttcc 
      aagacagtg caagccacg cctccacag cgccaagct 
      tcaaggaaa gaatttctc tattcgacg tacaatttt 
      cagtcgctt tggtaaact tgctggatc agattaata 
      atttaaaca aggatagca gtgaacacg acaggctct 
      ttaatttgt tatacaggc tggacttca attcgtttt 
      attctggcg atttaatga agaaggatt ttgggacca 
      tcacaattg aatcgtctg gttgattag cttagatgt 
      acaattttt tggtgtaat tttgattta catctataa 
      gttagggtt tatgtagaa tatttatgt tagcatagt 
      taattaata aaaaatcaa aaccgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY