Status
Please wait ...
Gene information
General information
TC007862 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1781673..1810333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007862
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06243

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007862 RA
      MLKFLTHKL RTHSINEDN YVEKADEDR DSGTESDEE 
      IDDPDLPPV MSERMCSVS PADTGCSME SPAPDQHDP 
      HSSEEELEV INSNKEERP ARPLRATSV CLPEKRKWS 
      QVGGDSYSR HKYTPENEE NMSHYGNLS PSSPCSPYH 
      KCYGEDGIH IEHSGSSDE EVHHLLASM ATPVQFRTS 
      PPLEAVKPG RKTPMNKGR SMSPPPKLL HYEESPRKR 
      SKHRHAHVQ RPYLDFEKM QHLKARSVR TWRHSGDHG 
      GELSVFCW
    CDS sequence
    • >TC007862 RA
      atgctcaag ttcctcacc cacaagctg cggacgcat 
      tcgataaac gaagacaat tacgtcgaa aaggctgat 
      gaagatcgc gattccggg accgaatcc gacgaggaa 
      attgacgac ccggactta cctccagtg atgtctgaa 
      cgcatgtgt agcgtatcc ccggcagac accggctgt 
      tccatggag agtccggcc cccgaccag cacgaccct 
      cacagctcc gaagaggag ctcgaggtg atcaacagc 
      aacaaagag gaacgtcca gctcgacca ctccgtgca 
      acgtccgtt tgtctgccg gaaaaacgt aaatggtcg 
      caagtcggc ggcgattcc tattctcgc cacaagtac 
      acgccagaa aacgaagaa aacatgtca cattatggc 
      aatctgagt ccgtcatcg ccgtgttct ccgtatcat 
      aaatgttac ggcgaggac gggatccac atcgaacat 
      tccggttct tcggacgaa gaagtccat catttactc 
      gccagtatg gcgacaccg gtccaattt cgcacctct 
      ccacccttg gaggccgtc aagccgggg cggaagaca 
      cccatgaac aagggccgc tccatgagt cctccgccc 
      aagctcttg cactacgag gagtccccg aggaagagg 
      tccaagcac aggcacgcg cacgtacag agaccttac 
      ttggacttc gaaaaaatg caacatctg aaagctcgc 
      tctgtgcgg acatggcgt cacagcgga gaccacggc 
      ggagagctt tcggtgttc tgttggtga
    mRNA sequence
    • >TC007862 RA
      ccgctccag acagactgt aaaagtttt ataacttag 
      gactaaacg agtccccat gtatccgtt tggtccatt 
      gagtaataa cgcgaattc tccggttac ttgaagctc 
      tcatcgaaa aaagccgcc cggatcgat tccggttaa 
      aaccggcgc caaacaaag gcgaaatcc tgcgtttgc 
      cgctccgcc tcaatggaa atgatttta gaaaaaagc 
      tcagaaagc atactaaag acatataaa gttggcacg 
      tccaagctc ggcgagctt ttggccggt tttctcttt 
      ctacctttg tgtaaccga gctgaaacg tgaacgaat 
      tctctctca aggaccggt tttagcttt acacttgta 
      gtttctagc ttacgctac gccatacgc cggagttag 
      ttaggcaat tatgcaact cctctgtgt gagacagaa 
      aagcattat gcagacgag aagagagca taacaagcc 
      ggcgaagac caatcacag accgcttgg gaatcagac 
      ctacctttg tcatcaatg ggttaccga cgagtccat 
      gtttgctgc cgggggagt ccgccaggc tttttccgg 
      gcgatttcg gccccagcg cccggattt ggcggggcg 
      gcgcccgga gcgcgcccc cggagccct ctcacgcgc 
      ttttcggcg gtctcgggc cccggggtg cggtatttc 
      cccggtttc ggggtcggt gcaagtatt gacttcccc 
      ccggtagta ttaatagac tgtttacgg cacgcagcg 
      cacttacta gacgtacaa tcagtagta gaacgtctc 
      gcttggagt gaggaggta caaagtgaa ttgactgaa 
      atgcactcc ggttgtcct tagggcgac gatgaaatc 
      gcgagtttg tgccgaatt tcagtgaat ccggtcaaa 
      taagcgccg tcaaggtgg cgacgaaac caggaagca 
      agtcccgga aggaaaata tgctcaagt tcctcaccc 
      acaagctgc ggacgcatt cgataaacg aagacaatt 
      acgtcgaaa aggctgatg aagatcgcg attccggga 
      ccgaatccg acgaggaaa ttgacgacc cggacttac 
      ctccagtga tgtctgaac gcatgtgta gcgtatccc 
      cggcagaca ccggctgtt ccatggaga gtccggccc 
      ccgaccagc acgaccctc acagctccg aagaggagc 
      tcgaggtga tcaacagca acaaagagg aacgtccag 
      ctcgaccac tccgtgcaa cgtccgttt gtctgccgg 
      aaaaacgta aatggtcgc aagtcggcg gcgattcct 
      attctcgcc acaagtaca cgccagaaa acgaagaaa 
      acatgtcac attatggca atctgagtc cgtcatcgc 
      cgtgttctc cgtatcata aatgttacg gcgaggacg 
      ggatccaca tcgaacatt ccggttctt cggacgaag 
      aagtccatc atttactcg ccagtatgg cgacaccgg 
      tccaatttc gcacctctc cacccttgg aggccgtca 
      agccggggc ggaagacac ccatgaaca agggccgct 
      ccatgagtc ctccgccca agctcttgc actacgagg 
      agtccccga ggaagaggt ccaagcaca ggcacgcgc 
      acgtacaga gaccttact tggacttcg aaaaaatgc 
      aacatctga aagctcgct ctgtgcgga catggcgtc 
      acagcggag accacggcg gagagcttt cggtgttct 
      gttggtgag aggagctta aagggcgct caccgtcgc 
      cctgcgaat tatcaaaaa ctccatgca tgagcagtt 
      taccagcgt tatggcccg accatcact caccaaacg 
      agtaaaagt gctcagtaa attgggtaa ttactggaa 
      aaagtggtg gcctgtaaa cttagtgct tgtatttat 
      gactgtaca taatttaaa tcaaatcac ctcaagttc 
      aatgtggta catttacat ggtctgaat aactacggc 
      tcttcattg cactataac ttacagcac tgaactttc 
      ggactactg aagattatc gcctgtgag cggccttct 
      cacctctat ttgcacttt cgcctaatt taccgacga 
      attcaacaa agttttgac aatatactt gtagaagca 
      gtgccacaa tcttcagta ctttaatgt tgtaacaat 
      atcatagtt gtaacagtt atatttaga ggaacgaat 
      tttcgaatt gagtcggtt ggttttagt gtttcttgt 
      taccaatta tcggtagct cataaggtt tccaggcct 
      acaggaaaa ttacgcgta cggacatag tgtttcaat 
      gtacaaaac gttatttcg gccaagcat aaaattaca 
      attaattac gaatttata ttgtagata tgagcattt 
      ttcgtgcaa tttactaat gttgtcgac ttttgtaca 
      taactgaaa ttgtgcatt tacaagcgt gtgcattat 
      ataaaaaca agagtgttt cgctttcag ttgttttgt 
      aaatactac cgttgtttt tatataatg ttaattatt 
      gcaatatga aatattttt acaaagtag ttgagtgtc 
      tgtagattt agttggact ttaggcccc aagccgcaa 
      acttcgcga gtttcgtcc aaaaatccg ttgccttta 
      attagagaa gacaaaatt attgttgcg atgcattta 
      ctgccattt ggaattttt caaaattca cgacggagc 
      tttggaagt ttgtgcgag tggggctaa taaattgtg 
      aatttattc taagccatg ttgttgcca tattaccgc 
      ttactgaac tttaacact ggccaggaa cagttcgaa 
      aacaacaat gctgccgaa caactgcct gttgaagac 
      aaacaacac ccaaatggt aactgccgg tggattggt 
      gaaatcaaa attaattta aagtggcgt tgaactcgc 
      aagatcatg cttataaga tagtaggac ttttaacaa 
      attgaaaga cttttttta aattgtgtg tgagtgagt 
      cgtgttttg taaaatgga aatgctgcg attttgtag 
      gatggagaa acctgaatt tgtcaaaaa atgtataat 
      caagagtag atactataa ggtgtgtat agtactata 
      atttttttt atataaaac aatcttgtg tgtagggta 
      aaactataa ttatttaaa cattgccca tttggaaca 
      tcggtgttg caccagttg attttttgt tgaatttgg 
      gaggttaca actacaaca ctcgaattt gatttcatg 
      tgattctaa ttagatcta agttttatc tacctatga 
      gatgggtaa taaattaac tacatttaa aaaccagtg 
      atatgtttt aagtatttt gcaccgtta cgtgcagtt 
      gtgagtaac atttttctt gtgatacaa actattaat 
      agccatatc gaaaccatc gcaaacttt gcgtttact 
      ttccatgtg gctattttg cacgttgta atacttagt 
      tgcaataca catattaag tttaacgat tttttaact 
      aacaaaatt acccaataa aatattttt tatcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY