Status
Please wait ...
Gene information
General information
TC007786 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 19719..24853
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007786
    iBeetle screen iB_06242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    female genital lobes slightly shortened - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007786 RA
      MSEDRRKMN RSSTGTVPK IKCRNNTTS CPNERDSPR 
      SWTNNLTGD FHRLNLSDY LRNSRRNHC AMNVGTVEE 
      QRLNPALNG SITPSSSSS RRNSSGTVP KCDNITKYP 
      DKRQIEDKL SQIQEYLQI TTSLMSSMK NTDDQADRN 
      DNYSESATS HHNDLDEKI DHTNREMEM LRDQQMSLL 
      CLQQKAENK LKDARQIQE KLLTASQYD VNSSINQRN 
      KNLSSVQDF DIAIRELEE RSKRLNEPR GSNSNLQDK 
      LLAEMDSLQ SQIVTMHNI NDDRNQLIQ VLDNRDTEL 
      RAQHLELQN KLSELQNKK SQVDQLVAQ LQVLEETDE 
      DDVGAQVRR IVTMKEQLS KLKDMLEIV KTTELNSNA 
      SQEEQDLAC GICTKAENF LQKDGEKRR TPLNKQVNK 
      NACDRGFGG AKPKSRTNS NKVALQVEL EAKKRELEE 
      IMGKHKAGT SNLNHDVGT DNKSEFSCS SNAYFDWAP 
      IMSDHNRFD SSDSDEYSD PNQQFVLPT LNYPKSDHD 
      YVAHHLQDT TTDRLSSIP RTPERRTPR AASEQGKNQ 
      VQKQLELIR SVCDSMLDQ QGGTNVRNN LTPSSLYFE 
      PRPMNTTAI VDPNYQSWL ATNTLQTQA FMLNTLNQC 
      CQMLWLQQR ELISLRQTV NQLQDYGGN LASPLQSEC 
      RSRSNQKGG NQVAAACSM PNLNQYNIP PSVNLDPSS 
      SYQNNMQNA RLLDHCLNN VSDHNNSVL HAVNANTNQ 
      IPPTHMWNG QALNNQVAP GNRANNYWD NFRSYSRQN 
      LLSTKSNEG FQNISNNPF TLVTTPKCN SDQMSSQEN 
      TPRRRTQFR NNNSVVPPD VLNVNKVDQ RNINECDYN 
      NESNSTISE EHTIFKESA SRRNEWHEE QVDDHHTKS 
      KLFEELREN VYKEVVTLI SANETRPHF LIQLFRDLQ 
      MISSDPLRL KILQAIQTI ITHNLTTNH TNRQVFEPQ 
      QSEPIDLQS TVWSKKIIK NQTATSRNT LDDNRVIKE 
      IIPFLNDHE DDSITHPFL VSLKQVLLE SESFQETVR 
      DSVFKKHFS NVLDEVLAQ YQGKKVHDV KMHFIQTVS 
      DLLRGELSF IQLIQDTCP DGGELETFE TNVNQEVTI 
      QNGDLAEAD QGRVEEDDG EVEEEGAVG GFWELPLEV 
      SEDVEVPST EFQNNAEAQ TQFIKQGLD QVPTRLPTK 
      SPSNTPSKD RSTSSRSDH HDLF
    CDS sequence
    • >TC007786 RA
      atgtctgaa gaccgcagg aagatgaac cgttcgagt 
      actggaacg gtgcctaaa atcaaatgc aggaataac 
      actacttcg tgccccaac gaacgtgat agtcctcgc 
      agttggacc aacaacttg acgggcgat ttccaccgc 
      ctcaacctc tctgactac ttgaggaat tccagacga 
      aatcattgc gccatgaac gtcggtacc gtcgaagag 
      cagcgcttg aatcctgcg ttaaacggt tccatcact 
      ccaagttca tccagctca cgccgtaac tcttcggga 
      acggtgccc aagtgcgac aacatcacc aagtatcca 
      gacaagaga caaattgaa gacaagttg agtcaaatc 
      caagagtat ctccaaatc accacttca ctcatgtcc 
      agtatgaag aatacagat gatcaggct gaccgcaat 
      gacaattac tcagagagt gcaacatca catcacaat 
      gaccttgac gaaaaaatc gaccataca aatcgcgaa 
      atggaaatg ttgagagac caacaaatg tcactgttg 
      tgtttacaa caaaaagcc gagaacaaa ttgaaagat 
      gctcgtcag atccaagaa aaattactg acggcttct 
      caatatgat gttaacagt agtatcaac caacgcaat 
      aagaatttg agcagtgtg caggacttt gatatcgcc 
      attcgagaa ttggaagag agaagtaaa agactgaat 
      gaacctcga ggttcaaat tcgaatctc caagacaag 
      cttttggcc gaaatggac agtctgcaa agtcagatc 
      gtcacaatg cacaacatc aacgacgat cgaaatcaa 
      ttaattcaa gttttggat aatcgagac accgaattg 
      cgagcccaa catttagaa ttgcaaaat aaattgagc 
      gaacttcag aataaaaaa tcacaagtt gatcaatta 
      gtggcgcaa ttgcaagtt ttggaagaa actgatgaa 
      gacgatgtt ggtgcacaa gtcaggaga atcgtaaca 
      atgaaagaa caattatcg aaattaaaa gatatgttg 
      gaaattgtc aaaactacg gagctgaat tcgaacgca 
      tcacaagaa gaacaagat ctagcatgt ggaatatgt 
      acgaaagcg gagaatttt ttacagaaa gatggggag 
      aaaagaaga actccgttg aataaacaa gtgaataag 
      aatgcatgt gatcggggt tttggaggt gctaagcct 
      aaaagtcga acgaatagt aataaagtg gcgcttcaa 
      gtggaacta gaagcaaaa aagagggaa ttggaagag 
      atcatgggc aaacataaa gctggtacg tcaaactta 
      aatcacgat gttggcact gacaataaa tcggaattc 
      agttgttca agtaatgcg tattttgat tgggcgcca 
      atcatgtcc gaccataat agatttgac tcaagtgac 
      agtgacgaa tattctgac ccgaaccaa caattcgtt 
      cttccaact ttaaactat cctaaatca gaccatgac 
      tatgttgcc catcactta caagacact actactgat 
      cgtctatca agtatacct cgaacacct gaacgtcgc 
      acaccgcgt gccgctagc gaacaggga aaaaatcaa 
      gtacaaaaa caattagaa ttgatacga agtgtttgc 
      gattctatg ttggaccag caaggggga acgaacgtc 
      agaaataat ctaactcca agttcatta tattttgaa 
      cccaggcct atgaacacc actgcgatt gtggatccc 
      aactatcaa agctggttg gcaacaaat actcttcaa 
      acacaagct ttcatgctg aatacgctc aaccaatgt 
      tgtcagatg ttgtggttg caacagcgc gaattgatt 
      tcgttgaga caaactgta aatcaattg caagattat 
      ggcggcaat ttggcttca cctttacaa agtgaatgt 
      cgctcaaga tcgaatcaa aaagggggg aatcaagtg 
      gctgcggca tgttcaatg ccaaattta aatcaatat 
      aatatccca ccttccgtc aatcttgat ccttcttct 
      tcttatcag aataacatg caaaatgct cgattgtta 
      gatcattgt ttgaataat gtgagtgat cataataac 
      agtgtgttg catgctgtt aatgctaac actaaccaa 
      ataccgcca actcatatg tggaatgga caagctcta 
      aataaccaa gtcgcgcct ggaaataga gctaacaat 
      tattgggac aattttaga agttattcc cgccaaaac 
      cttctttca acgaaaagt aatgaaggt ttccaaaat 
      atctccaat aatcctttt acattggta acaacacca 
      aaatgtaat tccgatcaa atgtcatct caagagaat 
      acaccacgt agaagaaca cagtttcga aataataat 
      tcagttgta ccacctgac gtcctcaat gttaataaa 
      gtcgatcag agaaacata aacgagtgc gattacaac 
      aatgaaagc aattcgacc atttcagaa gaacatacg 
      atttttaaa gaatcagca agtcgacga aacgaatgg 
      cacgaagag caagttgat gatcatcat accaagtcg 
      aaattgttt gaagaattg cgcgaaaac gtctacaaa 
      gaagtggtg actcttatt tcggccaat gagacacgt 
      ccacatttc ctcattcaa ttgtttcgt gatttacaa 
      atgatcagt tcggatccg ttacgactt aaaattcta 
      caggcaatt caaacaata attacgcat aatttaaca 
      acgaatcac acaaataga caagttttt gagccacaa 
      caaagcgaa ccgattgac ttgcaatca accgtttgg 
      tcaaaaaaa ataataaaa aatcaaaca gccacgtca 
      cgtaacact ttggatgat aatcgagtg ataaaagaa 
      ataattccg tttctcaac gatcacgag gacgatagt 
      ataactcat ccatttttg gtgagtcta aagcaagtt 
      ctactcgaa tctgagtcg tttcaagaa acagtgcga 
      gactcggtt ttcaagaaa catttttcc aatgttttg 
      gatgaggtt ttagcacag tatcagggc aaaaaagtt 
      catgacgtg aagatgcat tttattcaa accgtcagt 
      gatttattg agaggcgaa ttgtctttc attcagttg 
      atacaggat acgtgtcct gatggtggc gaactcgaa 
      acgtttgaa actaatgtt aatcaagaa gtaaccata 
      caaaatggt gatttagct gaagccgat caagggagg 
      gtggaagag gatgatggg gaagtggag gaggaagga 
      gcggttggg ggattttgg gaattaccg ttggaggtt 
      tctgaagat gtagaggtg ccatccacc gagtttcag 
      aacaatgca gaagcacag actcaattt attaagcaa 
      ggtttggat caagtaccg actcgtttg ccgactaaa 
      tccccttca aatactccg agtaaagat cggtcaact 
      tcatcacgt tccgatcat cacgatctg ttttaa
    mRNA sequence
    • >TC007786 RA
      attgtcaaa aagtagaca taaaatgat tgtcggatt 
      ttaaataaa aatagctgc aatattttc ctattgttt 
      gagttgtaa ttgcttcta atatgttag taaaagtgt 
      gtcttgaat aagccggtt cgcataata tgtctgaag 
      accgcagga agatgaacc gttcgagta ctggaacgg 
      tgcctaaaa tcaaatgca ggaataaca ctacttcgt 
      gccccaacg aacgtgata gtcctcgca gttggacca 
      acaacttga cgggcgatt tccaccgcc tcaacctct 
      ctgactact tgaggaatt ccagacgaa atcattgcg 
      ccatgaacg tcggtaccg tcgaagagc agcgcttga 
      atcctgcgt taaacggtt ccatcactc caagttcat 
      ccagctcac gccgtaact cttcgggaa cggtgccca 
      agtgcgaca acatcacca agtatccag acaagagac 
      aaattgaag acaagttga gtcaaatcc aagagtatc 
      tccaaatca ccacttcac tcatgtcca gtatgaaga 
      atacagatg atcaggctg accgcaatg acaattact 
      cagagagtg caacatcac atcacaatg accttgacg 
      aaaaaatcg accatacaa atcgcgaaa tggaaatgt 
      tgagagacc aacaaatgt cactgttgt gtttacaac 
      aaaaagccg agaacaaat tgaaagatg ctcgtcaga 
      tccaagaaa aattactga cggcttctc aatatgatg 
      ttaacagta gtatcaacc aacgcaata agaatttga 
      gcagtgtgc aggactttg atatcgcca ttcgagaat 
      tggaagaga gaagtaaaa gactgaatg aacctcgag 
      gttcaaatt cgaatctcc aagacaagc ttttggccg 
      aaatggaca gtctgcaaa gtcagatcg tcacaatgc 
      acaacatca acgacgatc gaaatcaat taattcaag 
      ttttggata atcgagaca ccgaattgc gagcccaac 
      atttagaat tgcaaaata aattgagcg aacttcaga 
      ataaaaaat cacaagttg atcaattag tggcgcaat 
      tgcaagttt tggaagaaa ctgatgaag acgatgttg 
      gtgcacaag tcaggagaa tcgtaacaa tgaaagaac 
      aattatcga aattaaaag atatgttgg aaattgtca 
      aaactacgg agctgaatt cgaacgcat cacaagaag 
      aacaagatc tagcatgtg gaatatgta cgaaagcgg 
      agaattttt tacagaaag atggggaga aaagaagaa 
      ctccgttga ataaacaag tgaataaga atgcatgtg 
      atcggggtt ttggaggtg ctaagccta aaagtcgaa 
      cgaatagta ataaagtgg cgcttcaag tggaactag 
      aagcaaaaa agagggaat tggaagaga tcatgggca 
      aacataaag ctggtacgt caaacttaa atcacgatg 
      ttggcactg acaataaat cggaattca gttgttcaa 
      gtaatgcgt attttgatt gggcgccaa tcatgtccg 
      accataata gatttgact caagtgaca gtgacgaat 
      attctgacc cgaaccaac aattcgttc ttccaactt 
      taaactatc ctaaatcag accatgact atgttgccc 
      atcacttac aagacacta ctactgatc gtctatcaa 
      gtatacctc gaacacctg aacgtcgca caccgcgtg 
      ccgctagcg aacagggaa aaaatcaag tacaaaaac 
      aattagaat tgatacgaa gtgtttgcg attctatgt 
      tggaccagc aagggggaa cgaacgtca gaaataatc 
      taactccaa gttcattat attttgaac ccaggccta 
      tgaacacca ctgcgattg tggatccca actatcaaa 
      gctggttgg caacaaata ctcttcaaa cacaagctt 
      tcatgctga atacgctca accaatgtt gtcagatgt 
      tgtggttgc aacagcgcg aattgattt cgttgagac 
      aaactgtaa atcaattgc aagattatg gcggcaatt 
      tggcttcac ctttacaaa gtgaatgtc gctcaagat 
      cgaatcaaa aagggggga atcaagtgg ctgcggcat 
      gttcaatgc caaatttaa atcaatata atatcccac 
      cttccgtca atcttgatc cttcttctt cttatcaga 
      ataacatgc aaaatgctc gattgttag atcattgtt 
      tgaataatg tgagtgatc ataataaca gtgtgttgc 
      atgctgtta atgctaaca ctaaccaaa taccgccaa 
      ctcatatgt ggaatggac aagctctaa ataaccaag 
      tcgcgcctg gaaatagag ctaacaatt attgggaca 
      attttagaa gttattccc gccaaaacc ttctttcaa 
      cgaaaagta atgaaggtt tccaaaata tctccaata 
      atcctttta cattggtaa caacaccaa aatgtaatt 
      ccgatcaaa tgtcatctc aagagaata caccacgta 
      gaagaacac agtttcgaa ataataatt cagttgtac 
      cacctgacg tcctcaatg ttaataaag tcgatcaga 
      gaaacataa acgagtgcg attacaaca atgaaagca 
      attcgacca tttcagaag aacatacga tttttaaag 
      aatcagcaa gtcgacgaa acgaatggc acgaagagc 
      aagttgatg atcatcata ccaagtcga aattgtttg 
      aagaattgc gcgaaaacg tctacaaag aagtggtga 
      ctcttattt cggccaatg agacacgtc cacatttcc 
      tcattcaat tgtttcgtg atttacaaa tgatcagtt 
      cggatccgt tacgactta aaattctac aggcaattc 
      aaacaataa ttacgcata atttaacaa cgaatcaca 
      caaatagac aagtttttg agccacaac aaagcgaac 
      cgattgact tgcaatcaa ccgtttggt caaaaaaaa 
      taataaaaa atcaaacag ccacgtcac gtaacactt 
      tggatgata atcgagtga taaaagaaa taattccgt 
      ttctcaacg atcacgagg acgatagta taactcatc 
      catttttgg tgagtctaa agcaagttc tactcgaat 
      ctgagtcgt ttcaagaaa cagtgcgag actcggttt 
      tcaagaaac atttttcca atgttttgg atgaggttt 
      tagcacagt atcagggca aaaaagttc atgacgtga 
      agatgcatt ttattcaaa ccgtcagtg atttattga 
      gaggcgaat tgtctttca ttcagttga tacaggata 
      cgtgtcctg atggtggcg aactcgaaa cgtttgaaa 
      ctaatgtta atcaagaag taaccatac aaaatggtg 
      atttagctg aagccgatc aagggaggg tggaagagg 
      atgatgggg aagtggagg aggaaggag cggttgggg 
      gattttggg aattaccgt tggaggttt ctgaagatg 
      tagaggtgc catccaccg agtttcaga acaatgcag 
      aagcacaga ctcaattta ttaagcaag gtttggatc 
      aagtaccga ctcgtttgc cgactaaat ccccttcaa 
      atactccga gtaaagatc ggtcaactt catcacgtt 
      ccgatcatc acgatctgt tttaattta ttcgttagt 
      ttatatcga gattatttt gaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY