Status
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Gene information
General information
TC007720 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 220316..226174
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007720
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007720 RA
      MTAICFVCN LPILSHQVG LVWQGGNGW DDLVREQVE 
      ESTIRQRLG GRRDSATQI CSISPPTET QETSPPTSG 
      TTRRRRSSL AQLTDILRE WGGSTKTRQ KQLCRRETL 
      ADLARSLPW GRQSTTEAG PTPSITTAR KRRESSADS 
      GIRSMGSKS RRDSHHTAI SDFRSEVAK LWTRRESTT 
      TMVTTSTSP RRGSGESSK SGRRDSVSH SHGTSRRGS 
      GESGRSGRR DSTTIITPP PPKIVAAQK KRRDSRTIN 
      DTPYYRQEQ RPSTSSTAS DSTPLVTTQ ETTTQVMSP 
      PTIITSSVT PPATSPTVQ ATTPTHPLL ATRRDSTTQ 
      CGRLGRRDS RSHASPERS SKLSRLQRQ NTAYDESCL 
      PPGGWSRRG SQPTALSPD VDDTGRKAR RDSLSPDSA 
      SRSRRDSRS HLSPDRSGE RDISPVRRT TRRGRLRRQ 
      STSVARGGG PRSPDSSSC SSRDPSPSN RPVPPPSEN 
      YRPTIRRQS TTEEILIAR GFRRQSTTE EMIRCRNFR 
      RQSSQSDDC QRYRGRRDS SAQILDGTI ASMTVETSS 
      TFFDSSTQT EPSPLYDNN HYHEECLRC NSCGLNLTG 
      PNQKRARRF KNQILCDLH FADVALMEC SDFMQQLRA 
      FKPQSLGCA VARRKSSTT LIFPLPPQA CSGKSKQQV 
      SSTFLLLT
    • >TC007720 RB
      MTAICFVCN LPILSHQVG LVWQGGNGW DDLVREQVE 
      ESTIRQRLG GRRDSATQI CSISPPTET QETSPPTSG 
      TTRRRRSSL AQLTDILRE WGGSTKTRQ KQLCRRETL 
      ADLARSLPW GRQSTTEAG PTPSITTAR KRRESSADS 
      GIRSMGSKS RRDSHHTAI SDFRSEVAK LWTRRESTT 
      TMVTTSTSP RRGSGESSK SGRRDSVSH SHGTSRRGS 
      GESGRSGRR DSTTIITPP PPKIVAAQK KRRDSRTIN 
      DTPYYRQEQ RPSTSSTAS DSTPLVTTQ ETTTQVMSP 
      PTIITSSVT PPATSPTVQ ATTPTHPLL ATRRDSTTQ 
      CGRLGRRDS RSHASPERS SKLSRLQRQ NTAYDESCL 
      PPGGWSRRG SQPTALSPD VDDTGRKAR RDSLSPDSA 
      SRSRRDSRS HLSPDRSGE RDISPVRRT TRRGRLRRQ 
      STSVARGGG PRSPDSSSC SSRDPSPSN RPVPPPSEN 
      YRPTIRRQS TTEEILIAR GFRRQSTTE EMIRCRNFR 
      RQSSQSDDC QRYRGRRDS SAQILDGTI ASMTVETSS 
      TFFDSSTQT VGNRSGGGA RRRDAAQWC RGIRSTWTS 
      TSWPFPPCT RTKLKTSLT SRWLLKCRE WPLTPSIEG 
      CVLLDYELS TAETPEPSP LYDNNHYHE ECLRCNSCG 
      LNLTGPNQK RARRFKNQI LCDLHFADV ALMECSDFM 
      QQLRAFKPQ SLGCAVARR KSSTTLIFP LPPQACSGK 
      SKQQVSSTF LLLT
    CDS sequence
    • >TC007720 RA
      atgacggcc atctgcttc gtgtgcaac cttccgatc 
      ctctcccac caagtcggt ctagtatgg caagggggc 
      aacggctgg gacgacctg gtgcgggaa caagtcgaa 
      gagtccacc atccgtcaa cgtctgggc ggtcgaaga 
      gactccgcc actcaaatc tgcagcatc tctccgccg 
      acggaaacc caagaaacc tccccaccc acctcgggc 
      acgacccgc cgccgtcgg agttccctc gcccaactc 
      acagacatc ctccgcgaa tggggcggt tcgacgaaa 
      acccgccaa aaacaactg tgtcggagg gaaactcta 
      gcggacttg gcacgttca ctcccctgg gggcggcaa 
      tccaccacc gaagccggc cccaccccc agcatcacc 
      accgcccgc aaacgaagg gaatctagc gctgactcc 
      ggcatcaga agcatgggc tccaagtcc aggagagac 
      tcgcaccac actgccatt tcagatttc cggtcggaa 
      gttgccaaa ttatggacg cggagggag tccaccacg 
      acgatggtc accaccagc acgtcgccg agacgtgga 
      agcggcgaa tcgagcaaa agtggacga agagattcc 
      gtctcacac tcacatgga acgtcaaga aggggctcg 
      ggggagagt ggacgtagt ggtcggaga gactccacc 
      actattatc acaccccca cctccgaaa atcgtagca 
      gcgcaaaaa aaacgccga gattcacgt acgattaac 
      gacactccg tattataga caggaacaa agaccgtcg 
      acttcgtcc actgcgtcc gattcaact cccttggtg 
      acgacccag gaaacaaca acccaggtc atgtcacct 
      ccaactatt ataacttca agtgttact ccaccggcg 
      acttcgcca acagttcaa gccacaact cccacacat 
      ccgttattg gcgaccaga cgtgactcc accacacag 
      tgtggtcgt ctaggacgt agagactcc cggagtcat 
      gctagtccc gaacgtagc agtaaacta tctcgtttg 
      cagcgtcaa aacaccgct tacgacgag agttgttta 
      ccacctggg ggttggtct cggcgaggt tcgcaaccc 
      acggcttta agtcccgat gtggacgac accggacgt 
      aaagcacgt cgtgactca ctaagtcca gattccgcc 
      tccaggagt cgaagagac tccaggtcg catctaagt 
      ccagatcgt tcgggtgaa agggatatt agcccagtt 
      agacgaacc acacgaaga gggcgttta cggcgccag 
      tcgacaagc gtggccaga ggtggggga ccacgttca 
      ccagacagt tcaagttgt tcaagtcga gatccaagt 
      ccttccaac cgacctgtt cctccccca agtgaaaat 
      taccgaccg acgattcga cggcagtca accaccgag 
      gagattttg atcgctcga gggttccga cgacagtcg 
      accacggag gagatgatc agatgtcgg aacttccgg 
      agacagagc tcccaaagt gacgattgc caaaggtat 
      aggggtagg agggattca agcgcacaa attctagac 
      ggtactatt gcttccatg actgttgaa acctccagc 
      acgtttttc gattccagt acgcaaaca gaaccttcg 
      ccgctgtac gacaataat cactaccac gaggaatgt 
      ttgcgatgc aattcgtgc ggactcaat ttgacgggt 
      ccgaaccag aagcgagca agacgcttc aaaaaccaa 
      atactctgc gacttacac ttcgctgac gttgctctc 
      atggagtgc tccgacttc atgcagcaa cttagggct 
      ttcaagcca caatcactg ggatgtgca gtggccaga 
      aggaagtct tcaactacc ctcattttc cctctacca 
      ccacaagcc tgttcaggt aagtcaaaa caacaagtt 
      tcttcaaca tttttattg ctcacgtaa
    • >TC007720 RB
      atgacggcc atctgcttc gtgtgcaac cttccgatc 
      ctctcccac caagtcggt ctagtatgg caagggggc 
      aacggctgg gacgacctg gtgcgggaa caagtcgaa 
      gagtccacc atccgtcaa cgtctgggc ggtcgaaga 
      gactccgcc actcaaatc tgcagcatc tctccgccg 
      acggaaacc caagaaacc tccccaccc acctcgggc 
      acgacccgc cgccgtcgg agttccctc gcccaactc 
      acagacatc ctccgcgaa tggggcggt tcgacgaaa 
      acccgccaa aaacaactg tgtcggagg gaaactcta 
      gcggacttg gcacgttca ctcccctgg gggcggcaa 
      tccaccacc gaagccggc cccaccccc agcatcacc 
      accgcccgc aaacgaagg gaatctagc gctgactcc 
      ggcatcaga agcatgggc tccaagtcc aggagagac 
      tcgcaccac actgccatt tcagatttc cggtcggaa 
      gttgccaaa ttatggacg cggagggag tccaccacg 
      acgatggtc accaccagc acgtcgccg agacgtgga 
      agcggcgaa tcgagcaaa agtggacga agagattcc 
      gtctcacac tcacatgga acgtcaaga aggggctcg 
      ggggagagt ggacgtagt ggtcggaga gactccacc 
      actattatc acaccccca cctccgaaa atcgtagca 
      gcgcaaaaa aaacgccga gattcacgt acgattaac 
      gacactccg tattataga caggaacaa agaccgtcg 
      acttcgtcc actgcgtcc gattcaact cccttggtg 
      acgacccag gaaacaaca acccaggtc atgtcacct 
      ccaactatt ataacttca agtgttact ccaccggcg 
      acttcgcca acagttcaa gccacaact cccacacat 
      ccgttattg gcgaccaga cgtgactcc accacacag 
      tgtggtcgt ctaggacgt agagactcc cggagtcat 
      gctagtccc gaacgtagc agtaaacta tctcgtttg 
      cagcgtcaa aacaccgct tacgacgag agttgttta 
      ccacctggg ggttggtct cggcgaggt tcgcaaccc 
      acggcttta agtcccgat gtggacgac accggacgt 
      aaagcacgt cgtgactca ctaagtcca gattccgcc 
      tccaggagt cgaagagac tccaggtcg catctaagt 
      ccagatcgt tcgggtgaa agggatatt agcccagtt 
      agacgaacc acacgaaga gggcgttta cggcgccag 
      tcgacaagc gtggccaga ggtggggga ccacgttca 
      ccagacagt tcaagttgt tcaagtcga gatccaagt 
      ccttccaac cgacctgtt cctccccca agtgaaaat 
      taccgaccg acgattcga cggcagtca accaccgag 
      gagattttg atcgctcga gggttccga cgacagtcg 
      accacggag gagatgatc agatgtcgg aacttccgg 
      agacagagc tcccaaagt gacgattgc caaaggtat 
      aggggtagg agggattca agcgcacaa attctagac 
      ggtactatt gcttccatg actgttgaa acctccagc 
      acgtttttc gattccagt acgcaaaca gttggtaat 
      cgatcggga ggtggggcc aggcgtcgc gacgctgct 
      cagtggtgc cgtggcatc cgcagcacg tggacgtcg 
      acgtcgtgg ccgtttcct ccatgcact cgaaccaaa 
      ctcaaaact agtctcaca agtcggtgg cttttaaaa 
      tgccgggag tggccgctg acgccctct attgaaggg 
      tgcgtcctt ctggactat gagctttca actgctgaa 
      acaccagaa ccttcgccg ctgtacgac aataatcac 
      taccacgag gaatgtttg cgatgcaat tcgtgcgga 
      ctcaatttg acgggtccg aaccagaag cgagcaaga 
      cgcttcaaa aaccaaata ctctgcgac ttacacttc 
      gctgacgtt gctctcatg gagtgctcc gacttcatg 
      cagcaactt agggctttc aagccacaa tcactggga 
      tgtgcagtg gccagaagg aagtcttca actaccctc 
      attttccct ctaccacca caagcctgt tcaggtaag 
      tcaaaacaa caagtttct tcaacattt ttattgctc 
      acgtaa
    mRNA sequence
    • >TC007720 RA
      gtcagccag aatgagcca tggcgcagc tgccttcgc 
      cgcgcttgc gccactaca gaacaatga cggccatct 
      gcttcgtgt gcaaccttc cgatcctct cccaccaag 
      tcggtctag tatggcaag ggggcaacg gctgggacg 
      acctggtgc gggaacaag tcgaagagt ccaccatcc 
      gtcaacgtc tgggcggtc gaagagact ccgccactc 
      aaatctgca gcatctctc cgccgacgg aaacccaag 
      aaacctccc cacccacct cgggcacga cccgccgcc 
      gtcggagtt ccctcgccc aactcacag acatcctcc 
      gcgaatggg gcggttcga cgaaaaccc gccaaaaac 
      aactgtgtc ggagggaaa ctctagcgg acttggcac 
      gttcactcc cctgggggc ggcaatcca ccaccgaag 
      ccggcccca cccccagca tcaccaccg cccgcaaac 
      gaagggaat ctagcgctg actccggca tcagaagca 
      tgggctcca agtccagga gagactcgc accacactg 
      ccatttcag atttccggt cggaagttg ccaaattat 
      ggacgcgga gggagtcca ccacgacga tggtcacca 
      ccagcacgt cgccgagac gtggaagcg gcgaatcga 
      gcaaaagtg gacgaagag attccgtct cacactcac 
      atggaacgt caagaaggg gctcggggg agagtggac 
      gtagtggtc ggagagact ccaccacta ttatcacac 
      ccccacctc cgaaaatcg tagcagcgc aaaaaaaac 
      gccgagatt cacgtacga ttaacgaca ctccgtatt 
      atagacagg aacaaagac cgtcgactt cgtccactg 
      cgtccgatt caactccct tggtgacga cccaggaaa 
      caacaaccc aggtcatgt cacctccaa ctattataa 
      cttcaagtg ttactccac cggcgactt cgccaacag 
      ttcaagcca caactccca cacatccgt tattggcga 
      ccagacgtg actccacca cacagtgtg gtcgtctag 
      gacgtagag actcccgga gtcatgcta gtcccgaac 
      gtagcagta aactatctc gtttgcagc gtcaaaaca 
      ccgcttacg acgagagtt gtttaccac ctgggggtt 
      ggtctcggc gaggttcgc aacccacgg ctttaagtc 
      ccgatgtgg acgacaccg gacgtaaag cacgtcgtg 
      actcactaa gtccagatt ccgcctcca ggagtcgaa 
      gagactcca ggtcgcatc taagtccag atcgttcgg 
      gtgaaaggg atattagcc cagttagac gaaccacac 
      gaagagggc gtttacggc gccagtcga caagcgtgg 
      ccagaggtg ggggaccac gttcaccag acagttcaa 
      gttgttcaa gtcgagatc caagtcctt ccaaccgac 
      ctgttcctc ccccaagtg aaaattacc gaccgacga 
      ttcgacggc agtcaacca ccgaggaga ttttgatcg 
      ctcgagggt tccgacgac agtcgacca cggaggaga 
      tgatcagat gtcggaact tccggagac agagctccc 
      aaagtgacg attgccaaa ggtataggg gtaggaggg 
      attcaagcg cacaaattc tagacggta ctattgctt 
      ccatgactg ttgaaacct ccagcacgt ttttcgatt 
      ccagtacgc aaacagaac cttcgccgc tgtacgaca 
      ataatcact accacgagg aatgtttgc gatgcaatt 
      cgtgcggac tcaatttga cgggtccga accagaagc 
      gagcaagac gcttcaaaa accaaatac tctgcgact 
      tacacttcg ctgacgttg ctctcatgg agtgctccg 
      acttcatgc agcaactta gggctttca agccacaat 
      cactgggat gtgcagtgg ccagaagga agtcttcaa 
      ctaccctca ttttccctc taccaccac aagcctgtt 
      caggtaagt caaaacaac aagtttctt caacatttt 
      tattgctca cgtaattac aatacaaga ctatctcta 
      ac
    • >TC007720 RB
      gtcttgcac aattttcct ggtgtagct taaataatc 
      gagcttttg ctactatga tatagttgg tgctagata 
      aaacaatga cggccatct gcttcgtgt gcaaccttc 
      cgatcctct cccaccaag tcggtctag tatggcaag 
      ggggcaacg gctgggacg acctggtgc gggaacaag 
      tcgaagagt ccaccatcc gtcaacgtc tgggcggtc 
      gaagagact ccgccactc aaatctgca gcatctctc 
      cgccgacgg aaacccaag aaacctccc cacccacct 
      cgggcacga cccgccgcc gtcggagtt ccctcgccc 
      aactcacag acatcctcc gcgaatggg gcggttcga 
      cgaaaaccc gccaaaaac aactgtgtc ggagggaaa 
      ctctagcgg acttggcac gttcactcc cctgggggc 
      ggcaatcca ccaccgaag ccggcccca cccccagca 
      tcaccaccg cccgcaaac gaagggaat ctagcgctg 
      actccggca tcagaagca tgggctcca agtccagga 
      gagactcgc accacactg ccatttcag atttccggt 
      cggaagttg ccaaattat ggacgcgga gggagtcca 
      ccacgacga tggtcacca ccagcacgt cgccgagac 
      gtggaagcg gcgaatcga gcaaaagtg gacgaagag 
      attccgtct cacactcac atggaacgt caagaaggg 
      gctcggggg agagtggac gtagtggtc ggagagact 
      ccaccacta ttatcacac ccccacctc cgaaaatcg 
      tagcagcgc aaaaaaaac gccgagatt cacgtacga 
      ttaacgaca ctccgtatt atagacagg aacaaagac 
      cgtcgactt cgtccactg cgtccgatt caactccct 
      tggtgacga cccaggaaa caacaaccc aggtcatgt 
      cacctccaa ctattataa cttcaagtg ttactccac 
      cggcgactt cgccaacag ttcaagcca caactccca 
      cacatccgt tattggcga ccagacgtg actccacca 
      cacagtgtg gtcgtctag gacgtagag actcccgga 
      gtcatgcta gtcccgaac gtagcagta aactatctc 
      gtttgcagc gtcaaaaca ccgcttacg acgagagtt 
      gtttaccac ctgggggtt ggtctcggc gaggttcgc 
      aacccacgg ctttaagtc ccgatgtgg acgacaccg 
      gacgtaaag cacgtcgtg actcactaa gtccagatt 
      ccgcctcca ggagtcgaa gagactcca ggtcgcatc 
      taagtccag atcgttcgg gtgaaaggg atattagcc 
      cagttagac gaaccacac gaagagggc gtttacggc 
      gccagtcga caagcgtgg ccagaggtg ggggaccac 
      gttcaccag acagttcaa gttgttcaa gtcgagatc 
      caagtcctt ccaaccgac ctgttcctc ccccaagtg 
      aaaattacc gaccgacga ttcgacggc agtcaacca 
      ccgaggaga ttttgatcg ctcgagggt tccgacgac 
      agtcgacca cggaggaga tgatcagat gtcggaact 
      tccggagac agagctccc aaagtgacg attgccaaa 
      ggtataggg gtaggaggg attcaagcg cacaaattc 
      tagacggta ctattgctt ccatgactg ttgaaacct 
      ccagcacgt ttttcgatt ccagtacgc aaacagttg 
      gtaatcgat cgggaggtg gggccaggc gtcgcgacg 
      ctgctcagt ggtgccgtg gcatccgca gcacgtgga 
      cgtcgacgt cgtggccgt ttcctccat gcactcgaa 
      ccaaactca aaactagtc tcacaagtc ggtggcttt 
      taaaatgcc gggagtggc cgctgacgc cctctattg 
      aagggtgcg tccttctgg actatgagc tttcaactg 
      ctgaaacac cagaacctt cgccgctgt acgacaata 
      atcactacc acgaggaat gtttgcgat gcaattcgt 
      gcggactca atttgacgg gtccgaacc agaagcgag 
      caagacgct tcaaaaacc aaatactct gcgacttac 
      acttcgctg acgttgctc tcatggagt gctccgact 
      tcatgcagc aacttaggg ctttcaagc cacaatcac 
      tgggatgtg cagtggcca gaaggaagt cttcaacta 
      ccctcattt tccctctac caccacaag cctgttcag 
      gtaagtcaa aacaacaag tttcttcaa catttttat 
      tgctcacgt aattacaat acaagacta tctctaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY