General information | |||
TC031587
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450524.1: 4..20229 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC031587 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02971 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04345 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04346 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05363 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06239 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC031587 RA
MGRHRYRGN VDSDDSDSS DDEPRPKRS TPGVSTTVN SPPVSAESA RIARLEQLV ENLNRRLLN RNFEHTSGQ VGIKSDLIP EFAPGNPNF STTKWLHKI DQLALVNGW DERTTIYGM ISRLKGLAK EWYDNLDPS AYDRSWDEW KRLLQATFP DHHDYASTL RKLVNRVKE DGETWAQYY FGKLNLLQA CDITGTNAV SCLIDGITD VTLRNGARA GRYVTPESL YAEYLSTLR SEGDKRDTL AKQAPRERR RFLPSRVEA QKLYSSVRC YNCRNRGHV ATKCPKPRI ECKKCGRIG HVDAECRRG NVVKENMSK QALTTSVAD ASNNKNYYI DIKVNGKPT RAYIDSGAE PVLVKQSVA RELGLMWQP SLHLIRGYG QGITKVHGE VEVELEVDL VKANVKALI VEDSAQRVP VIVGQTFLN AGANTIIAN EEAVRVVDG NNESIQAFL GQLPTRKVA LWAEEETVI PPQSIGCIR IKAKDDGWK GAYYVEWCQ RPRPGFEHV VHRCVTCDG GLVTVRNLS HQDLVYRKA QIVARAEPC EQERTATTV SVFSVGKVE VKSFQRWEL LTQVNKNLD PAQFEQLLQ LVNEFRDCF ARNVAEIGA TTAAEMKLT LTDDKPVIY RPYRLSHHE RRIVRDIVN DLLGSGVIR ESDSPYSSP ILLVRKKDG QHRMCVDYR QLNSKTIKD RFPLPRVDE HLDKLKGAK FFTTLDLAS GYFQIPMAT ESIPKTAFV TPDGHCEFV RMPFGLANA PAVFQRAMN KVLGPLQFQ TAFCYIDDL LIPSKDFET GLNNLQTVF QLLRQFGLT LKLSKYCFF GSQIEYLGH EISAEGIKP GETKIKAVT AFPKPTDVH KLRQFLGLC GYFRKYVKD YATIANSLT SLLKNGSAF VLEEVQERA FQTLKDILT SRPVLAIYD AEAETELHT DASKVGIGG ILLQRQGDG SLRPVMFFS RQTTKEEQR YHSYELETL AVVCSLKHY RVYLLGLQF KVITDCNAL RTTLTKRDL IPRIGRWWL LTSEFDFTV EYRPGSKMS HVDALSRNT VLDPSEPSV EVLHINVND DDWILAVQM KDARCALLK EILEKSPTN AEERAIHKE YKLKDGRVF KQTESGLKW VVPKAVRRQ ILLAHHDGI GHLSNEKTL ASVSKSYWF PSMRSKKFK THCTDLNVK LVLTATATP RANGQVERL NRTILSQLA ASSREEEKW DDFVSRISW GINSTPNST TGKSPYELL LGYTPRHAN DSILTNVVT EGVHDGDLP RTRADVAQR VEKEQQKQK ERYDKRRCA PKRFNAGDL VLVRTTRVS NEGKSRKLL PKYSGPYRI QQVLDYDRY VVTDMPGAT RSQKRYEGV HSVDKLRHY VVATTSGDE TIGDELRNH TLSIFIWVW LKRREKFSG EIFAMGRHR YRGNVDSDD SDSSDDEPR PKRSAPGVS TTVNSPPVS AESARIARL EQLVENLNR RLLNRNFEH TSGQVGIKS DLIPEFAPG NPNFSTTKW LHKIDQLAL VNGWDERTT IYGMISRLK GLAKEWYDN LDPSAYDRS WDEWKRLLQ ATFPDHHDY ASTLLSCLI DGMTDVTLR NGARAGRYV TPESLYAEY LSTLRSEGD KRDTLAKQA PRERRRFLP SRVEAQKLY SSVRCYNCR NRGHVATKC PKPRIECKK CGRIGHVDA ECRRGNVVK ENMSKQALT TSVADASNN KNYYIDIKV NGKPTRAYI DSGAEPVLV KQSVARELG LMWQPSLHL IRGYGQGIT KVHGEVEVE LEVDLVKAN VKALIVEDS AQRVPVIVG QTFLNAGAN TIIANEEAV RVVDGNNES IQAFLGQLP TRKVALWAE EETVIPPQS IGCIRIKAK DDGWKGAYY VEGCQRPRP GFEHVVHRC VTCDGGLVT VRNLSHQDL VYRKAQIVA RAEPCEQER TATTVSVFS VGKVEVKSF QRWELLTQV NKNLDPAQF EQLLQLVNE FRDCFARNV AEIGATTVA EMKLTLTDD KPVIYRPYR LSHHERRIV RDIVNDLLG SGVIRESDS PYSSPILLV RKKDGQHRM CVDYRQLNS KTIKDRFPL PRVDEHLDK LNGAKFFTT LDLASGYYQ IPMATESIP KTAFVTPDG HYEFVRMPF GLANAPAVF QRAMNKVLG PLRFQTAFC YIDDLLIPS KDFETGLNN LRTVFQLLR QFGLTLKLS KCCFFGSQI EYLGHEISA EGIKPGETK IKAVTAFPK PTDVHKLRQ FLGLCGYFR KYVKDYATI ANSLTSLLK KGSAFVWEE AQERAFQTL KDILTSRPV LAIYDAEAE TELHTDASK VGIGGILLQ RQGDGSLRP VMFFSRQTT KEEQRYHSY ELETLAVVC SLKHYRVYL LGLQFKVIT DCNALRTTL TKRDLIPRI GRWWLLTSE FDFTVEYRP GSKMSHVDA LSRNPVLDP SEPSVEVLH INVNDDDWI LAVQMKDAR CALLKEILE KSPTNAEER AIHKEYKLK DGRVFKQTE SGLKWVVPK AVRRQILLA HHDGIGHLS NEKTLASVS KSYWFPSMR SKKFKTHCT DLNVKLVLT ATATPRANG QVERLNRTI LSQLAASSR EEEKWDDFV SRISWGINS TPNSTTGKS PYELLLGYT PRHANDSIL TNVVTEGVH DGDLPRTRA DVAQRVEKE QQKQKERYD KRRCAPKRF NAGDLVLVR TTRASNEGK SRKLLPKYS GPYRIQKVL DYDRYRGNV DSDDSDSSD DEPRPKRSA PGVSTTVNS PPVSAESAR IARLEQLVE NLNRRLLNR NFEHTSGQV GIKSDLIPE FAPGNPNFS TTKWLHKID QLALVNGWD ERTTIYGMI SRLKGLAKE WYDNLDPSA YDRSWDEWK LSCLIDGMT DVTLRNGAR AGRYVTPES LYAEYLSTL RSEGDKRDT LAKQAPRER RRFLPSRVE TQKLYSARN QELSARNVV ALDMWMLSR RGNLVKENM SKQALTTSV ADASNNKNY YIDIKVNGK PTRAYIDSG AEPVLVKQS VARELGLMW QPSLHLIRG YGQGITKVH GEVEVELEV DLVKANVKA LIVEDSAQR VPVIVGQTF LNAGANTII ANEEAVRVV DGNNESIQA FLGQLPTRK VALWAEEET VIPPQSIGC IRIKAKDDG KKFKTHCTD LNVKLVLTA TATPRANGQ VERLNRTIL SQLAASSRE EEKWDDFVS RISWGINST PNSTTGKSP YELLLGYTP RHANDSILT NVVTEGVHD GDLPRTRAD VAQRVEKEQ QKQKERYDK RRCAPKRFN AGDLVLVRT TRASNEGKS RKLLPKYSG PYRIQKVLD YDRYVVTDM PGATRSQKR YEGVHSVDK LRHYVVATT SGDETIGDE LRNHTLSIF IWVWLKRRE KFSGEIFAM GRHRYRGNV DSDDSDSSD DEPRPKRSA PGVSTTVNS PPVSAESAR IARLEQLVE NLNRRLLNR NFEHTSGQV GIKSGLIPE FAPGNPNFS TTKWLHKID QLALVNGWD ERTTIYGMI SRLKGLAKE WYDNLDPSA YDRSWDEWK RLLQATFPD HHDYASTLR KLVNRVKED GETWAQYYF GKLNLLQAC DITGTNAVS CLIDGITDV TLRNGARAG RYVTPESLY AEYLSTLRS EGDKRDTLA KQAPRERRR FLPSRVEAQ KLYSSVRCY NCRNRGHVA TKCPKPRIE CKKCGRIGH VDAECRRGN VVKENMSKQ ALTTSVADA SNNKNYYID IKVNGKPTR AYIDSGAEP VLVKQSVAR ELGLMWQPS LHLIRGYGQ GITKVHGEV EVELEVDLV KANVKALIV EDSAQRVPV IVGQTFLNA GANTIIANE EAVRVVDGN NESIQAFLG QLPTRKVAL WAEDETVIP PQSIGCIRI KAKDDGWKG AYYVEGCQR PRPGFEHVV HRCVTCDGG LVTVRNLSH QDLVYRKAQ IVARAEPCE QEWTATTLA ASSREEEKW DDFVSRISW GINSTPNST TGKSPYELL LGYTPRHAN DSILTNVVT EGVHDGDLP RTRADVAQR VEKEQQKQK ERYDKRRCA PKRFNAGDL VLVRTTRAS NEGKSRKLL PKYSGPYRI QKVLDYDRY VVTDMPGAT RSQKRYEGV HSVDKLRHY VVATTSGDE TIGDVSDE
- >TC031587 RA
atggggcgt cacaggtat cgaggaaat gtggactcg gacgactcg gactcaagt gacgatgag ccacggccc aaaaggtcc acgcctggg gtctccact acagtaaat tcaccccca gtttcggcc gagtccgct agaatcgcg agacttgag caacttgtg gaaaacttg aatcgacgt ttgctaaac cgcaatttt gagcacaca tcaggacaa gttggcatt aaaagtgac ctgattcct gaattcgct ccaggaaac cccaatttt agtaccact aagtggttg cataaaatt gaccagttg gctctcgtg aacggttgg gacgaacgt acaacgatt tatggcatg ataagtcga ctcaagggg ttggccaag gagtggtac gacaatctc gatccctca gcatacgac cggagctgg gatgaatgg aaacgtttg ttacaagcc accttcccc gatcaccac gattatgct tccacactt cgtaagcta gtgaatagg gtgaaggaa gacggtgaa acctgggca caatattat tttgggaaa ttaaatctg ttgcaggct tgtgacata acaggaacg aatgcagtc tcctgtctt attgatgga atcactgat gtgactctc agaaatggg gctagagca gggcgttac gttacaccc gaaagcttg tacgctgag tatttgtcg actctgaga tccgaaggt gacaagcgg gatacgctc gcaaagcag gcgcctcgg gaaaggagg aggttcctt ccaagtcga gtcgaagca caaaaactg tactcgtca gttcggtgt tacaattgt cgtaatcga ggacatgtt gcgacaaag tgcccgaaa ccaagaatt gagtgcaag aaatgtggt cgcattgga catgtggat gctgagtgc cgacgtggt aacgtagta aaagaaaac atgtctaag caggcacta acgacaagt gtagctgac gcaagtaat aataagaat tattacata gacattaag gtcaatggt aagccgacg cgagcttac attgactca ggagctgaa ccggtcctt gtcaagcaa agtgttgct agagaattg ggactgatg tggcagcca agtttacat ttgattcgt ggttatggc cagggaatc accaaagtg catggggaa gtcgaagta gagctagaa gtggatctt gtcaaggca aacgtaaaa gctttgatc gtcgaagac agtgcacaa cgtgtgcct gtcatagtg ggacagacc ttcctgaat gcgggtgct aatacgatt atagcaaat gaggaagct gtgagagta gttgacgga aacaacgaa tcgatccaa gcatttcta ggccaactt ccgacacga aaagttgca ctttgggcg gaagaggaa acagtgatc cctcctcaa tccattggt tgcatacga atcaaggca aaagacgat gggtggaaa ggagcttat tacgttgaa tggtgtcaa cgtccgaga ccagggttc gaacacgtt gttcatcgg tgtgtcaca tgtgatgga ggcctagtg accgttcgc aatctatcg caccaagat ctggtctat cgaaaggcg caaatcgtt gcgagagct gagccttgc gagcaggaa aggacagct acgacggtg agtgtgttt tctgtgggg aaggttgag gtaaagagt tttcaacgg tgggaactt cttacacaa gtcaataag aatctagac ccggctcag ttcgagcaa ttgttacaa ctcgttaac gaatttcga gattgtttt gcacgaaat gtagcggaa ataggtgcc acaactgcg gcagaaatg aagctaact ttgactgat gataaacct gtaatttat cgtccttat cggttatcg catcacgaa agaagaatt gttcgagat atagtaaat gacttgtta ggtagcgga gtcatacga gaatcagac tcgccatat tcgagtccc atactgttg gtgcggaag aaagatggg caacaccgt atgtgtgtt gactatcga caattaaat tcgaaaaca atcaaagat cgctttccg ttaccacga gtagatgaa catttggat aaactaaaa ggtgcaaag tttttcacc acactagat ctcgcgagt gggtacttt cagatccca atggccacc gaatcgatt ccaaagaca gcatttgtt acgcctgat gggcattgc gagtttgtc cgcatgcct tttggtcta gccaacgct ccggccgtg tttcagcga gctatgaat aaggtgttg ggtccatta cagtttcag accgctttt tgttacata gatgatctt ctgatacct tccaaagac tttgaaaca ggtcttaac aatttacaa acggtgttt caattgctt cgacagttc ggattgact ctaaagctg agtaaatat tgcttcttt ggaagtcaa atagagtat ttagggcat gagatcagt gcggaaggc attaagcca ggcgagaca aaaatcaaa gcggtgaca gcttttccc aaaccaaca gacgtacac aaacttagg caattctta ggtttgtgt gggtacttt cgaaagtac gtgaaagat tacgcaaca atagcaaac agtttgaca agtctccta aagaatggc agtgcattt gttttggaa gaagtacaa gaacgggct tttcagact ttaaaagac atcttgacg agcagacca gttcttgca atttacgac gcggaagct gaaacggag ttacacact gacgcttct aaagttgga attggtggc attctgttg caacgacaa ggtgatgga tctttgcgt ccagttatg tttttcagc cgacagacg accaaagag gaacaaagg taccactcg tacgagcta gaaacgcta gcggtggtc tgttctctc aaacattat cgagtatac ttgttgggg cttcaattc aaggtgatc acagactgt aatgcttta cgaacaacc cttaccaag agagatttg attccacga attgggaga tggtggttg ttgacttct gagttcgac tttactgta gaatacaga cctggcagt aaaatgagt cacgttgac gctttgagt agaaatacg gtattagat ccttcggag ccttcagtg gaagttttg catattaat gttaacgat gatgattgg attttagct gttcaaatg aaggatgcg agatgcgca ttgttgaag gaaatattg gaaaaatca ccgactaac gctgaagag cgtgccatt cacaaagag tataaacta aaagatggt cgagtattc aaacagact gagtcagga ttaaaatgg gtcgtacca aaagcggta agacgccag attttatta gcacaccac gatggaatt ggccattta tcgaatgag aaaactttg gcatctgtg tcgaaatct tattggttt ccttcaatg cggagcaaa aagtttaag acacattgt accgattta aacgttaaa ttagtacta accgcgact gccacacca cgggctaat ggccaggtg gagcgatta aatcgtacg attttatct cagcttgct gctagctcg agagaagaa gagaaatgg gatgacttt gtgagtaga attagttgg ggtattaat tctacgccg aattcgact acgggcaaa agtccatac gaattgttg ttggggtat acacctcga catgctaat gactcaatc ctcaccaac gtggtgaca gagggtgtt catgatggc gatttgcca cggacacga gcagacgtt gctcagcgt gtcgaaaag gaacagcag aagcaaaag gagcgttat gacaagcga cgctgtgcc ccaaagaga tttaatgca ggtgatctc gtgttggta cgcaccacg cgagtttca aacgagggc aaaagtcgg aagctgtta cctaaatat tcgggaccg taccgaatt cagcaagtg ctcgactac gataggtat gtcgtgacg gacatgccg ggggcgacg cgttcccaa aaacgatat gagggtgtt cactcagtg gacaaattg agacattac gttgtcgcc acgacgagt ggagacgaa acaataggc gatgagtta cgcaaccac acgctgtca attttcatt tgggtgtgg ctcaaaagg cgcgaaaag ttttctggt gagattttc gcaatgggg cgtcacagg taccgagga aatgtggac tcggacgac tcggactca agtgacgat gagccacgg ccgaaaagg tccgcgcct ggggtctcc actacagta aattcaccc ccagtttcg gccgagtcc gctagaatc gcgagactt gagcaactt gtggaaaac ttgaatcga cgtttgcta aaccgcaat tttgagcac acatcagga caagttggc attaaaagt gacctgatt cctgaattc gctccagga aaccccaat tttagtacc actaagtgg ttgcataaa attgaccag ttggctctc gtgaacggt tgggacgaa cgtacaacg atttacggc atgataagt cgactcaag gggttggcc aaggagtgg tacgacaat ctcgatccc tcagcatac gaccggagc tgggatgaa tggaaacgt ttgttacaa gccaccttc cccgatcac cacgattat gcttccaca cttctctcc tgtcttatt gatggaatg actgacgtg actctcaga aatggggct agagcaggg cgttacgtt acacccgaa agcttgtac gctgagtat ttgtcgact ctgagatcc gaaggtgac aagcgggat acgctcgca aagcaggcg cctcgggaa aggaggagg ttccttcca agtcgagtt gaagcacaa aaactgtat tcgtcagtt cggtgttac aattgtcgt aatcgagga catgttgcg acaaagtgc ccgaaacca agaattgag tgcaagaaa tgtggtcgc attggacat gtggatgct gagtgccga cgtggtaac gtagtaaaa gaaaacatg tctaagcag gcactaacg acaagtgta gctgacgca agtaataat aagaattat tacatagac attaaggtc aatggtaag ccgacgcga gcttacatt gactcagga gctgaaccg gtcctggtc aagcaaagt gttgctaga gaattggga ctgatgtgg cagccaagt ttacatttg attcgtggt tatggccag ggaatcacc aaagtgcat ggggaagtc gaagtagag ctagaagtg gatcttgtc aaggcaaac gtaaaagct ttgatcgtc gaagacagt gcacaacgt gtgcctgtc atagtggga cagaccttc ctgaatgcg ggtgctaat acgattata gcaaatgag gaagctgtg agagtagtt gacggaaac aacgaatcg atccaagca tttctaggc caacttccg acacgaaaa gttgcactt tgggcggaa gaggaaaca gtgatccct cctcaatcc attggttgc atacgaatc aaggcaaaa gacgatggg tggaaagga gcttattac gttgaaggg tgtcaacgt ccgagacca gggttcgaa cacgttgtt catcggtgt gtcacatgt gatggaggc ctagtgacc gttcgcaat ctatcgcac caagatctg gtctatcga aaggcgcaa atcgttgcg agagctgag ccttgcgag caggaaagg acagctacg acggtgagt gtgttttct gtggggaag gttgaggta aagagtttt caacggtgg gaacttctt acacaagtc aataagaat ctagacccg gctcagttc gagcaattg ttacaactc gttaacgaa tttcgagat tgttttgca cgaaatgta gcggaaata ggtgccaca actgtggca gaaatgaag ctaactttg actgatgat aaacctgta atttatcgt ccttatcgg ttatcgcat cacgaaaga agaattgtt cgagatata gtaaatgac ttgttaggt agcggagtc atacgagaa tcagactcg ccatattcg agtcccata ctgttggtg cggaagaaa gatgggcaa caccgtatg tgtgttgac tatcggcaa ttaaattcg aaaacaatc aaagatcgc tttccgtta ccacgagta gatgaacat ttggataaa ctaaacggt gcaaagttt ttcaccaca ctagatctc gcgagtggg tactatcag atcccaatg gccaccgaa tcgattcca aagacagca tttgttacg cctgatggg cattacgag tttgtccgc atgcctttt ggtctagcc aacgctccg gcagtgttt cagcgagct atgaataag gtgttgggt ccattacgg tttcagacc gctttttgt tacatagat gatcttctg ataccttcc aaagacttt gaaacaggt cttaacaat ttacgaacg gtgtttcaa ttgcttcga cagttcgga ttgactcta aagctgagt aaatgttgc ttctttgga agtcaaata gagtattta gggcatgag atcagtgcg gaaggcatt aagccaggc gagacaaaa atcaaagcg gtgacagct tttcccaaa ccaacagac gtacacaaa cttaggcaa ttcttaggt ttgtgtggg tactttcga aagtacgtg aaagattac gcaacaata gcaaacagt ttgacgagt ctcctaaag aaaggcagt gcatttgtt tgggaagaa gcacaagag cgggctttt cagacttta aaagacatc ttgacgagc agaccagtt cttgcaatt tacgacgcg gaagctgaa acggagtta cacactgac gcttctaaa gttggaatt ggtggcatt ctgttgcaa cgacaaggt gatggatct ttgcgtcca gttatgttt ttcagccga cagacgacc aaagaggaa caaaggtac cactcgtac gagctagaa acgctagcg gtggtctgt tctctcaaa cattatcga gtatacttg ttggggctt caattcaag gtgatcaca gactgtaat gctttacga acaaccctt accaagaga gatttgatt ccacgaatt gggagatgg tggttgttg acttctgag ttcgacttt actgtagaa tacagacct ggcagtaaa atgagtcac gttgacgct ttgagtaga aatccggta ttagatcct tcggagcct tcagtggaa gtcttgcat attaatgtt aacgatgat gattggatt ttagctgtt caaatgaag gatgcgaga tgcgcattg ttgaaggaa atattggaa aaatcaccg acgaacgct gaagagcgt gccattcac aaagagtat aaactaaaa gatggtcga gtattcaaa cagactgag tcaggatta aaatgggtc gtaccaaaa gcggtaaga cgccagatt ttattagca caccacgat ggaattggc catttatcg aatgagaaa actttggca tctgtgtcg aaatcttat tggtttcct tcaatgcgg agcaaaaag tttaagaca cattgtacc gatttaaac gttaaatta gtactaacc gcgactgct acaccacgg gctaacggc caggtggag cgattgaat cgtacgatt ttatctcag cttgctgct agctcgaga gaagaagag aaatgggat gactttgtg agtagaatt agttggggt attaattct acgccgaat tcgactacg ggcaaaagt ccatacgaa ttgttgttg gggtataca cctcgacat gctaatgac tcaatcctc accaacgtg gtgacagag ggtgttcat gatggtgat ttgccacgg acacgagca gacgttgct cagcgtgtc gaaaaggaa cagcagaag caaaaagag cgttatgac aagcgacgc tgtgcccca aagagattt aatgcaggt gatctcgtg ttggtacgc accacgcga gcttcaaac gagggcaaa agtcggaag ctgttacct aaatattcg ggaccgtac cgaattcag aaagtgctc gactacgat aggtaccga ggaaatgtg gactcggac gactcggac tcaagtgac gatgagcca cggccgaaa aggtccgcg cctggggtc tccactaca gtaaattca cccccagtt tcggccgag tccgctaga atcgcgaga cttgagcaa cttgtggaa aacttgaat cgacgtttg ctaaaccgt aattttgag cacacatca ggacaagtt ggcattaaa agtgacttg attcctgaa ttcgctcca ggaaacccc aattttagt accactaag tggttgcat aaaattgac cagttggct ctcgtgaac ggttgggac gaacgtaca acgatttat ggcatgata agtcgactc aaggggttg gccaaggag tggtacgac aatctcgat ccctcagca tatgaccgg agctgggat gaatggaaa ctctcctgt cttattgat ggaatgact gacgtgact ctcagaaat ggggctaga gcagggcgt tacgttaca cccgaaagc ttgtacgct gagtatttg tcgactctg agatccgaa ggtgacaag cgggatacg ctcgcaaag caggcgcct cgggaaagg aggaggttc cttccaagt cgagtcgaa acacaaaaa ctgtattct gcccgaaac caagaattg agtgcaaga aatgtggtc gcattggac atgtggatg cttagccga cgtggtaac ttagtaaaa gaaaacatg tctaagcag gcactaacg acaagtgta gctgacgca agtaataat aagaattat tacatagac attaaggtc aatggcaag ccgacgcga gcttacatt gactcagga gctgaaccg gtccttgtc aagcaaagt gttgctaga gaattggga ctgatgtgg cagccaagt ttacatttg attcgtggt tatggccag ggaatcacc aaagtgcat ggggaagtc gaagtagag ctagaagtg gatcttgtc aaggcaaac gtaaaagct ttgatcgtc gaagacagt gcacaacgt gtgcctgtc atagtggga cagaccttc ctgaatgcg ggtgctaat acgattata gcaaatgag gaagctgtg agagtagtt gacggaaac aacgaatcg atccaagca tttctaggc caacttccg acacgaaaa gttgcactt tgggcggaa gaggaaaca gtgatccct cctcaatcc attggttgc atacgaatc aaggcaaaa gacgatggc aaaaagttt aagacacat tgtaccgat ttaaacgtt aaattagta ctaaccgcg actgctaca ccacgggct aacggccag gtggagcga ttgaatcgt acgatttta tctcagctt gctgctagc tcgagagaa gaagagaaa tgggatgac tttgtgagt agaattagt tggggtatt aattctacg ccgaattcg actacgggc aaaagtcca tacgaattg ttgttgggg tatacacct cgacatgct aatgactca atcctcacc aacgtggtg acagagggt gttcatgat ggtgatttg ccacggaca cgagcagac gttgctcag cgtgtcgaa aaggaacag cagaagcaa aaagagcgt tatgacaag cgacgctgt gccccaaag agatttaat gcaggtgat ctcgtgttg gtacgcacc acgcgagct tcaaacgag ggcaaaagt cggaagctg ttacctaaa tattcggga ccgtaccga attcagaaa gtgctcgac tacgatagg tacgtcgtg acggacatg ccgggggcg acgcgttcc caaaaacga tatgagggt gttcactca gtggacaaa ttgagacat tacgttgtc gccacgacg agtggagac gaaacaata ggcgatgag ttacgcaac cacacgctg tcaattttc atttgggtg tggctcaaa aggcgcgaa aagttttct ggtgagatt ttcgcaatg gggcgtcac aggtaccga ggaaatgtg gactcggac gactcggac tcaagtgac gatgagcca cggccgaaa aggtccgcg cctggggtc tccactaca gtaaattca cccccagtt tcggccgag tccgctaga atcgcgaga cttgagcaa cttgtggaa aacttgaat cgacgtttg ctaaaccgc aattttgag cacacatca ggacaagtt ggcattaaa agtggcctg attcctgaa ttcgctcca ggaaacccc aattttagt accactaag tggttgcat aaaattgac cagttggct ctcgtgaac ggttgggac gaacgtaca acgatttac ggcatgata agtcgactc aaggggttg gccaaggag tggtacgac aatctcgat ccctcagca tacgaccgg agctgggat gaatggaaa cgtttgtta caagccacc ttccccgat caccacgat tatgcttcc acacttcgt aagctagtg aatagggtg aaggaagat ggggaaacc tgggcacaa tattatttt gggaaatta aatctgttg caggcttgt gacataaca ggaacgaat gcagtctcc tgtcttatt gatggaatc actgacgtg actctcaga aatggggct agagcaggg cgttacgtt acacccgaa agcttgtac gctgagtat ttgtcgact ctgagatcc gaaggtgac aagcgggat acgctcgca aagcaggcg cctcgggaa aggaggagg ttccttcca agtcgagtc gaagcacaa aaactgtat tcgtcagtt cggtgttac aattgtcgt aatcgagga catgttgcg acaaagtgc ccgaaacca agaattgag tgcaagaaa tgtggtcgc attggacat gtggatgct gagtgccga cgtggtaac gtagtaaaa gaaaacatg tctaagcag gcactaacg acaagtgta gctgacgca agtaataat aagaattat tacatagac attaaggtt aatggtaag ccgacgcga gcttacatt gactcagga gctgaaccg gtccttgtc aagcaaagt gttgctaga gaattggga ctgatgtgg cagccaagt ttacatttg attcgtggt tatggccag ggaatcacc aaagtgcat ggggaagtc gaagtagag ctagaagtg gatcttgtc aaggcaaac gtaaaagct ttgatcgtc gaagacagt gcacaacgt gtgcctgtc atagtggga cagaccttc ctgaatgcg ggtgctaat acgattata gcaaatgaa gaagctgtg agagtagtt gacggaaac aacgaatcg atccaagca tttctaggc caacttccg acacgaaaa gttgcactt tgggcggaa gacgaaaca gtgatccct cctcaatcc attggttgc atacgaatc aaggcaaaa gacgatggg tggaaagga gcttattac gttgaaggg tgtcaacgt ccgagacca gggttcgaa cacgttgtt catcggtgt gtcacatgt gatggaggc ctagtgacc gttcgcaat ctatcgcac caagatctg gtctatcga aaggcgcaa atcgttgcg agagctgag ccttgcgag caggaatgg acagctacg acgcttgct gctagctcg agagaagaa gagaaatgg gatgacttt gtgagtaga attagttgg ggtattaat tctacgccg aattcgact acgggcaaa agtccatac gaattgttg ttggggtat acacctcga catgctaat gactcaatc ctcaccaac gtggtgaca gagggtgtt catgatggt gatttgcca cggacacga gcagacgtt gctcagcgt gtcgaaaag gaacagcag aagcaaaaa gagcgttat gacaagcga cgctgtgcc ccaaagaga tttaatgca ggtgatctc gtgttggta cgcaccacg cgagcttca aacgagggc aaaagtcgg aagctgtta cctaaatat tcgggaccg taccgaatt cagaaagtg ctcgactac gataggtac gtcgtgacg gacatgccg ggggcgacg cgttcccaa aaacgatat gagggtgtt cactcagtg gacaaattg agacattac gttgtcgcc acgacgagt ggagacgaa acaataggc gatgtcagt gacgaatga
- >TC031587 RA
agattttta aagttatta taaatccaa acgactgtg atatattac aactgtaga tttaaatag tttttgagt tacgcaacc acacgctgt caattttca tttgggtgt ggctcaaaa ggcgcgaaa agttttcta atgagattt tcgcaatgg ggcgtcaca ggtatcgag gaaatgtgg actcggacg actcggact caagtgacg atgagccac ggcccaaaa ggtccacgc ctggggtct ccactacag taaattcac ccccagttt cggccgagt ccgctagaa tcgcgagac ttgagcaac ttgtggaaa acttgaatc gacgtttgc taaaccgca attttgagc acacatcag gacaagttg gcattaaaa gtgacctga ttcctgaat tcgctccag gaaacccca attttagta ccactaagt ggttgcata aaattgacc agttggctc tcgtgaacg gttgggacg aacgtacaa cgatttatg gcatgataa gtcgactca aggggttgg ccaaggagt ggtacgaca atctcgatc cctcagcat acgaccgga gctgggatg aatggaaac gtttgttac aagccacct tccccgatc accacgatt atgcttcca cacttcgta agctagtga atagggtga aggaagacg gtgaaacct gggcacaat attattttg ggaaattaa atctgttgc aggcttgtg acataacag gaacgaatg cagtctcct gtcttattg atggaatca ctgatgtga ctctcagaa atggggcta gagcagggc gttacgtta cacccgaaa gcttgtacg ctgagtatt tgtcgactc tgagatccg aaggtgaca agcgggata cgctcgcaa agcaggcgc ctcgggaaa ggaggaggt tccttccaa gtcgagtcg aagcacaaa aactgtact cgtcagttc ggtgttaca attgtcgta atcgaggac atgttgcga caaagtgcc cgaaaccaa gaattgagt gcaagaaat gtggtcgca ttggacatg tggatgctg agtgccgac gtggtaacg tagtaaaag aaaacatgt ctaagcagg cactaacga caagtgtag ctgacgcaa gtaataata agaattatt acatagaca ttaaggtca atggtaagc cgacgcgag cttacattg actcaggag ctgaaccgg tccttgtca agcaaagtg ttgctagag aattgggac tgatgtggc agccaagtt tacatttga ttcgtggtt atggccagg gaatcacca aagtgcatg gggaagtcg aagtagagc tagaagtgg atcttgtca aggcaaacg taaaagctt tgatcgtcg aagacagtg cacaacgtg tgcctgtca tagtgggac agaccttcc tgaatgcgg gtgctaata cgattatag caaatgagg aagctgtga gagtagttg acggaaaca acgaatcga tccaagcat ttctaggcc aacttccga cacgaaaag ttgcacttt gggcggaag aggaaacag tgatccctc ctcaatcca ttggttgca tacgaatca aggcaaaag acgatgggt ggaaaggag cttattacg ttgaatggt gtcaacgtc cgagaccag ggttcgaac acgttgttc atcggtgtg tcacatgtg atggaggcc tagtgaccg ttcgcaatc tatcgcacc aagatctgg tctatcgaa aggcgcaaa tcgttgcga gagctgagc cttgcgagc aggaaagga cagctacga cggtgagtg tgttttctg tggggaagg ttgaggtaa agagttttc aacggtggg aacttctta cacaagtca ataagaatc tagacccgg ctcagttcg agcaattgt tacaactcg ttaacgaat ttcgagatt gttttgcac gaaatgtag cggaaatag gtgccacaa ctgcggcag aaatgaagc taactttga ctgatgata aacctgtaa tttatcgtc cttatcggt tatcgcatc acgaaagaa gaattgttc gagatatag taaatgact tgttaggta gcggagtca tacgagaat cagactcgc catattcga gtcccatac tgttggtgc ggaagaaag atgggcaac accgtatgt gtgttgact atcgacaat taaattcga aaacaatca aagatcgct ttccgttac cacgagtag atgaacatt tggataaac taaaaggtg caaagtttt tcaccacac tagatctcg cgagtgggt actttcaga tcccaatgg ccaccgaat cgattccaa agacagcat ttgttacgc ctgatgggc attgcgagt ttgtccgca tgccttttg gtctagcca acgctccgg ccgtgtttc agcgagcta tgaataagg tgttgggtc cattacagt ttcagaccg ctttttgtt acatagatg atcttctga taccttcca aagactttg aaacaggtc ttaacaatt tacaaacgg tgtttcaat tgcttcgac agttcggat tgactctaa agctgagta aatattgct tctttggaa gtcaaatag agtatttag ggcatgaga tcagtgcgg aaggcatta agccaggcg agacaaaaa tcaaagcgg tgacagctt ttcccaaac caacagacg tacacaaac ttaggcaat tcttaggtt tgtgtgggt actttcgaa agtacgtga aagattacg caacaatag caaacagtt tgacaagtc tcctaaaga atggcagtg catttgttt tggaagaag tacaagaac gggcttttc agactttaa aagacatct tgacgagca gaccagttc ttgcaattt acgacgcgg aagctgaaa cggagttac acactgacg cttctaaag ttggaattg gtggcattc tgttgcaac gacaaggtg atggatctt tgcgtccag ttatgtttt tcagccgac agacgacca aagaggaac aaaggtacc actcgtacg agctagaaa cgctagcgg tggtctgtt ctctcaaac attatcgag tatacttgt tggggcttc aattcaagg tgatcacag actgtaatg ctttacgaa caaccctta ccaagagag atttgattc cacgaattg ggagatggt ggttgttga cttctgagt tcgacttta ctgtagaat acagacctg gcagtaaaa tgagtcacg ttgacgctt tgagtagaa atacggtat tagatcctt cggagcctt cagtggaag ttttgcata ttaatgtta acgatgatg attggattt tagctgttc aaatgaagg atgcgagat gcgcattgt tgaaggaaa tattggaaa aatcaccga ctaacgctg aagagcgtg ccattcaca aagagtata aactaaaag atggtcgag tattcaaac agactgagt caggattaa aatgggtcg taccaaaag cggtaagac gccagattt tattagcac accacgatg gaattggcc atttatcga atgagaaaa ctttggcat ctgtgtcga aatcttatt ggtttcctt caatgcgga gcaaaaagt ttaagacac attgtaccg atttaaacg ttaaattag tactaaccg cgactgcca caccacggg ctaatggcc aggtggagc gattaaatc gtacgattt tatctcagc ttgctgcta gctcgagag aagaagaga aatgggatg actttgtga gtagaatta gttggggta ttaattcta cgccgaatt cgactacgg gcaaaagtc catacgaat tgttgttgg ggtatacac ctcgacatg ctaatgact caatcctca ccaacgtgg tgacagagg gtgttcatg atggcgatt tgccacgga cacgagcag acgttgctc agcgtgtcg aaaaggaac agcagaagc aaaaggagc gttatgaca agcgacgct gtgccccaa agagattta atgcaggtg atctcgtgt tggtacgca ccacgcgag tttcaaacg agggcaaaa gtcggaagc tgttaccta aatattcgg gaccgtacc gaattcagc aagtgctcg actacgata ggtatgtcg tgacggaca tgccggggg cgacgcgtt cccaaaaac gatatgagg gtgttcact cagtggaca aattgagac attacgttg tcgccacga cgagtggag acgaaacaa taggcgatg agttacgca accacacgc tgtcaattt tcatttggg tgtggctca aaaggcgcg aaaagtttt ctggtgaga ttttcgcaa tggggcgtc acaggtacc gaggaaatg tggactcgg acgactcgg actcaagtg acgatgagc cacggccga aaaggtccg cgcctgggg tctccacta cagtaaatt cacccccag tttcggccg agtccgcta gaatcgcga gacttgagc aacttgtgg aaaacttga atcgacgtt tgctaaacc gcaattttg agcacacat caggacaag ttggcatta aaagtgacc tgattcctg aattcgctc caggaaacc ccaatttta gtaccacta agtggttgc ataaaattg accagttgg ctctcgtga acggttggg acgaacgta caacgattt acggcatga taagtcgac tcaaggggt tggccaagg agtggtacg acaatctcg atccctcag catacgacc ggagctggg atgaatgga aacgtttgt tacaagcca ccttccccg atcaccacg attatgctt ccacacttc tctcctgtc ttattgatg gaatgactg acgtgactc tcagaaatg gggctagag cagggcgtt acgttacac ccgaaagct tgtacgctg agtatttgt cgactctga gatccgaag gtgacaagc gggatacgc tcgcaaagc aggcgcctc gggaaagga ggaggttcc ttccaagtc gagttgaag cacaaaaac tgtattcgt cagttcggt gttacaatt gtcgtaatc gaggacatg ttgcgacaa agtgcccga aaccaagaa ttgagtgca agaaatgtg gtcgcattg gacatgtgg atgctgagt gccgacgtg gtaacgtag taaaagaaa acatgtcta agcaggcac taacgacaa gtgtagctg acgcaagta ataataaga attattaca tagacatta aggtcaatg gtaagccga cgcgagctt acattgact caggagctg aaccggtcc tggtcaagc aaagtgttg ctagagaat tgggactga tgtggcagc caagtttac atttgattc gtggttatg gccagggaa tcaccaaag tgcatgggg aagtcgaag tagagctag aagtggatc ttgtcaagg caaacgtaa aagctttga tcgtcgaag acagtgcac aacgtgtgc ctgtcatag tgggacaga ccttcctga atgcgggtg ctaatacga ttatagcaa atgaggaag ctgtgagag tagttgacg gaaacaacg aatcgatcc aagcatttc taggccaac ttccgacac gaaaagttg cactttggg cggaagagg aaacagtga tccctcctc aatccattg gttgcatac gaatcaagg caaaagacg atgggtgga aaggagctt attacgttg aagggtgtc aacgtccga gaccagggt tcgaacacg ttgttcatc ggtgtgtca catgtgatg gaggcctag tgaccgttc gcaatctat cgcaccaag atctggtct atcgaaagg cgcaaatcg ttgcgagag ctgagcctt gcgagcagg aaaggacag ctacgacgg tgagtgtgt tttctgtgg ggaaggttg aggtaaaga gttttcaac ggtgggaac ttcttacac aagtcaata agaatctag acccggctc agttcgagc aattgttac aactcgtta acgaatttc gagattgtt ttgcacgaa atgtagcgg aaataggtg ccacaactg tggcagaaa tgaagctaa ctttgactg atgataaac ctgtaattt atcgtcctt atcggttat cgcatcacg aaagaagaa ttgttcgag atatagtaa atgacttgt taggtagcg gagtcatac gagaatcag actcgccat attcgagtc ccatactgt tggtgcgga agaaagatg ggcaacacc gtatgtgtg ttgactatc ggcaattaa attcgaaaa caatcaaag atcgctttc cgttaccac gagtagatg aacatttgg ataaactaa acggtgcaa agtttttca ccacactag atctcgcga gtgggtact atcagatcc caatggcca ccgaatcga ttccaaaga cagcatttg ttacgcctg atgggcatt acgagtttg tccgcatgc cttttggtc tagccaacg ctccggcag tgtttcagc gagctatga ataaggtgt tgggtccat tacggtttc agaccgctt tttgttaca tagatgatc ttctgatac cttccaaag actttgaaa caggtctta acaatttac gaacggtgt ttcaattgc ttcgacagt tcggattga ctctaaagc tgagtaaat gttgcttct ttggaagtc aaatagagt atttagggc atgagatca gtgcggaag gcattaagc caggcgaga caaaaatca aagcggtga cagcttttc ccaaaccaa cagacgtac acaaactta ggcaattct taggtttgt gtgggtact ttcgaaagt acgtgaaag attacgcaa caatagcaa acagtttga cgagtctcc taaagaaag gcagtgcat ttgtttggg aagaagcac aagagcggg cttttcaga ctttaaaag acatcttga cgagcagac cagttcttg caatttacg acgcggaag ctgaaacgg agttacaca ctgacgctt ctaaagttg gaattggtg gcattctgt tgcaacgac aaggtgatg gatctttgc gtccagtta tgtttttca gccgacaga cgaccaaag aggaacaaa ggtaccact cgtacgagc tagaaacgc tagcggtgg tctgttctc tcaaacatt atcgagtat acttgttgg ggcttcaat tcaaggtga tcacagact gtaatgctt tacgaacaa cccttacca agagagatt tgattccac gaattggga gatggtggt tgttgactt ctgagttcg actttactg tagaataca gacctggca gtaaaatga gtcacgttg acgctttga gtagaaatc cggtattag atccttcgg agccttcag tggaagtct tgcatatta atgttaacg atgatgatt ggattttag ctgttcaaa tgaaggatg cgagatgcg cattgttga aggaaatat tggaaaaat caccgacga acgctgaag agcgtgcca ttcacaaag agtataaac taaaagatg gtcgagtat tcaaacaga ctgagtcag gattaaaat gggtcgtac caaaagcgg taagacgcc agattttat tagcacacc acgatggaa ttggccatt tatcgaatg agaaaactt tggcatctg tgtcgaaat cttattggt ttccttcaa tgcggagca aaaagttta agacacatt gtaccgatt taaacgtta aattagtac taaccgcga ctgctacac cacgggcta acggccagg tggagcgat tgaatcgta cgattttat ctcagcttg ctgctagct cgagagaag aagagaaat gggatgact ttgtgagta gaattagtt ggggtatta attctacgc cgaattcga ctacgggca aaagtccat acgaattgt tgttggggt atacacctc gacatgcta atgactcaa tcctcacca acgtggtga cagagggtg ttcatgatg gtgatttgc cacggacac gagcagacg ttgctcagc gtgtcgaaa aggaacagc agaagcaaa aagagcgtt atgacaagc gacgctgtg ccccaaaga gatttaatg caggtgatc tcgtgttgg tacgcacca cgcgagctt caaacgagg gcaaaagtc ggaagctgt tacctaaat attcgggac cgtaccgaa ttcagaaag tgctcgact acgataggt accgaggaa atgtggact cggacgact cggactcaa gtgacgatg agccacggc cgaaaaggt ccgcgcctg gggtctcca ctacagtaa attcacccc cagtttcgg ccgagtccg ctagaatcg cgagacttg agcaacttg tggaaaact tgaatcgac gtttgctaa accgtaatt ttgagcaca catcaggac aagttggca ttaaaagtg acttgattc ctgaattcg ctccaggaa accccaatt ttagtacca ctaagtggt tgcataaaa ttgaccagt tggctctcg tgaacggtt gggacgaac gtacaacga tttatggca tgataagtc gactcaagg ggttggcca aggagtggt acgacaatc tcgatccct cagcatatg accggagct gggatgaat ggaaactct cctgtctta ttgatggaa tgactgacg tgactctca gaaatgggg ctagagcag ggcgttacg ttacacccg aaagcttgt acgctgagt atttgtcga ctctgagat ccgaaggtg acaagcggg atacgctcg caaagcagg cgcctcggg aaaggagga ggttccttc caagtcgag tcgaaacac aaaaactgt attctgccc gaaaccaag aattgagtg caagaaatg tggtcgcat tggacatgt ggatgctta gccgacgtg gtaacttag taaaagaaa acatgtcta agcaggcac taacgacaa gtgtagctg acgcaagta ataataaga attattaca tagacatta aggtcaatg gcaagccga cgcgagctt acattgact caggagctg aaccggtcc ttgtcaagc aaagtgttg ctagagaat tgggactga tgtggcagc caagtttac atttgattc gtggttatg gccagggaa tcaccaaag tgcatgggg aagtcgaag tagagctag aagtggatc ttgtcaagg caaacgtaa aagctttga tcgtcgaag acagtgcac aacgtgtgc ctgtcatag tgggacaga ccttcctga atgcgggtg ctaatacga ttatagcaa atgaggaag ctgtgagag tagttgacg gaaacaacg aatcgatcc aagcatttc taggccaac ttccgacac gaaaagttg cactttggg cggaagagg aaacagtga tccctcctc aatccattg gttgcatac gaatcaagg caaaagacg atggcaaaa agtttaaga cacattgta ccgatttaa acgttaaat tagtactaa ccgcgactg ctacaccac gggctaacg gccaggtgg agcgattga atcgtacga ttttatctc agcttgctg ctagctcga gagaagaag agaaatggg atgactttg tgagtagaa ttagttggg gtattaatt ctacgccga attcgacta cgggcaaaa gtccatacg aattgttgt tggggtata cacctcgac atgctaatg actcaatcc tcaccaacg tggtgacag agggtgttc atgatggtg atttgccac ggacacgag cagacgttg ctcagcgtg tcgaaaagg aacagcaga agcaaaaag agcgttatg acaagcgac gctgtgccc caaagagat ttaatgcag gtgatctcg tgttggtac gcaccacgc gagcttcaa acgagggca aaagtcgga agctgttac ctaaatatt cgggaccgt accgaattc agaaagtgc tcgactacg ataggtacg tcgtgacgg acatgccgg gggcgacgc gttcccaaa aacgatatg agggtgttc actcagtgg acaaattga gacattacg ttgtcgcca cgacgagtg gagacgaaa caataggcg atgagttac gcaaccaca cgctgtcaa ttttcattt gggtgtggc tcaaaaggc gcgaaaagt tttctggtg agattttcg caatggggc gtcacaggt accgaggaa atgtggact cggacgact cggactcaa gtgacgatg agccacggc cgaaaaggt ccgcgcctg gggtctcca ctacagtaa attcacccc cagtttcgg ccgagtccg ctagaatcg cgagacttg agcaacttg tggaaaact tgaatcgac gtttgctaa accgcaatt ttgagcaca catcaggac aagttggca ttaaaagtg gcctgattc ctgaattcg ctccaggaa accccaatt ttagtacca ctaagtggt tgcataaaa ttgaccagt tggctctcg tgaacggtt gggacgaac gtacaacga tttacggca tgataagtc gactcaagg ggttggcca aggagtggt acgacaatc tcgatccct cagcatacg accggagct gggatgaat ggaaacgtt tgttacaag ccaccttcc ccgatcacc acgattatg cttccacac ttcgtaagc tagtgaata gggtgaagg aagatgggg aaacctggg cacaatatt attttggga aattaaatc tgttgcagg cttgtgaca taacaggaa cgaatgcag tctcctgtc ttattgatg gaatcactg acgtgactc tcagaaatg gggctagag cagggcgtt acgttacac ccgaaagct tgtacgctg agtatttgt cgactctga gatccgaag gtgacaagc gggatacgc tcgcaaagc aggcgcctc gggaaagga ggaggttcc ttccaagtc gagtcgaag cacaaaaac tgtattcgt cagttcggt gttacaatt gtcgtaatc gaggacatg ttgcgacaa agtgcccga aaccaagaa ttgagtgca agaaatgtg gtcgcattg gacatgtgg atgctgagt gccgacgtg gtaacgtag taaaagaaa acatgtcta agcaggcac taacgacaa gtgtagctg acgcaagta ataataaga attattaca tagacatta aggttaatg gtaagccga cgcgagctt acattgact caggagctg aaccggtcc ttgtcaagc aaagtgttg ctagagaat tgggactga tgtggcagc caagtttac atttgattc gtggttatg gccagggaa tcaccaaag tgcatgggg aagtcgaag tagagctag aagtggatc ttgtcaagg caaacgtaa aagctttga tcgtcgaag acagtgcac aacgtgtgc ctgtcatag tgggacaga ccttcctga atgcgggtg ctaatacga ttatagcaa atgaagaag ctgtgagag tagttgacg gaaacaacg aatcgatcc aagcatttc taggccaac ttccgacac gaaaagttg cactttggg cggaagacg aaacagtga tccctcctc aatccattg gttgcatac gaatcaagg caaaagacg atgggtgga aaggagctt attacgttg aagggtgtc aacgtccga gaccagggt tcgaacacg ttgttcatc ggtgtgtca catgtgatg gaggcctag tgaccgttc gcaatctat cgcaccaag atctggtct atcgaaagg cgcaaatcg ttgcgagag ctgagcctt gcgagcagg aatggacag ctacgacgc ttgctgcta gctcgagag aagaagaga aatgggatg actttgtga gtagaatta gttggggta ttaattcta cgccgaatt cgactacgg gcaaaagtc catacgaat tgttgttgg ggtatacac ctcgacatg ctaatgact caatcctca ccaacgtgg tgacagagg gtgttcatg atggtgatt tgccacgga cacgagcag acgttgctc agcgtgtcg aaaaggaac agcagaagc aaaaagagc gttatgaca agcgacgct gtgccccaa agagattta atgcaggtg atctcgtgt tggtacgca ccacgcgag cttcaaacg agggcaaaa gtcggaagc tgttaccta aatattcgg gaccgtacc gaattcaga aagtgctcg actacgata ggtacgtcg tgacggaca tgccggggg cgacgcgtt cccaaaaac gatatgagg gtgttcact cagtggaca aattgagac attacgttg tcgccacga cgagtggag acgaaacaa taggcgatg tcagtgacg aatgactga gttcgctgt gaagctgag tgaagttca acgtgaact cacgatttt gggcccaaa tgttgtttt taagcgctt tacgttgaa actcat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
