Status
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Gene information
General information
TC031587
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012975,TC012973,TC012972,TC012974 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450524.1: 4..20229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031587
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02971

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    iBeetle screen iB_04346

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    iBeetle screen iB_05363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    iBeetle screen iB_06239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031587 RA
      MGRHRYRGN VDSDDSDSS DDEPRPKRS TPGVSTTVN 
SPPVSAESA RIARLEQLV ENLNRRLLN RNFEHTSGQ 
VGIKSDLIP EFAPGNPNF STTKWLHKI DQLALVNGW 
DERTTIYGM ISRLKGLAK EWYDNLDPS AYDRSWDEW 
KRLLQATFP DHHDYASTL RKLVNRVKE DGETWAQYY 
FGKLNLLQA CDITGTNAV SCLIDGITD VTLRNGARA 
GRYVTPESL YAEYLSTLR SEGDKRDTL AKQAPRERR 
RFLPSRVEA QKLYSSVRC YNCRNRGHV ATKCPKPRI 
ECKKCGRIG HVDAECRRG NVVKENMSK QALTTSVAD 
ASNNKNYYI DIKVNGKPT RAYIDSGAE PVLVKQSVA 
RELGLMWQP SLHLIRGYG QGITKVHGE VEVELEVDL 
VKANVKALI VEDSAQRVP VIVGQTFLN AGANTIIAN 
EEAVRVVDG NNESIQAFL GQLPTRKVA LWAEEETVI 
PPQSIGCIR IKAKDDGWK GAYYVEWCQ RPRPGFEHV 
VHRCVTCDG GLVTVRNLS HQDLVYRKA QIVARAEPC 
EQERTATTV SVFSVGKVE VKSFQRWEL LTQVNKNLD 
PAQFEQLLQ LVNEFRDCF ARNVAEIGA TTAAEMKLT 
LTDDKPVIY RPYRLSHHE RRIVRDIVN DLLGSGVIR 
ESDSPYSSP ILLVRKKDG QHRMCVDYR QLNSKTIKD 
RFPLPRVDE HLDKLKGAK FFTTLDLAS GYFQIPMAT 
ESIPKTAFV TPDGHCEFV RMPFGLANA PAVFQRAMN 
KVLGPLQFQ TAFCYIDDL LIPSKDFET GLNNLQTVF 
QLLRQFGLT LKLSKYCFF GSQIEYLGH EISAEGIKP 
GETKIKAVT AFPKPTDVH KLRQFLGLC GYFRKYVKD 
YATIANSLT SLLKNGSAF VLEEVQERA FQTLKDILT 
SRPVLAIYD AEAETELHT DASKVGIGG ILLQRQGDG 
SLRPVMFFS RQTTKEEQR YHSYELETL AVVCSLKHY 
RVYLLGLQF KVITDCNAL RTTLTKRDL IPRIGRWWL 
LTSEFDFTV EYRPGSKMS HVDALSRNT VLDPSEPSV 
EVLHINVND DDWILAVQM KDARCALLK EILEKSPTN 
AEERAIHKE YKLKDGRVF KQTESGLKW VVPKAVRRQ 
ILLAHHDGI GHLSNEKTL ASVSKSYWF PSMRSKKFK 
THCTDLNVK LVLTATATP RANGQVERL NRTILSQLA 
ASSREEEKW DDFVSRISW GINSTPNST TGKSPYELL 
LGYTPRHAN DSILTNVVT EGVHDGDLP RTRADVAQR 
VEKEQQKQK ERYDKRRCA PKRFNAGDL VLVRTTRVS 
NEGKSRKLL PKYSGPYRI QQVLDYDRY VVTDMPGAT 
RSQKRYEGV HSVDKLRHY VVATTSGDE TIGDELRNH 
TLSIFIWVW LKRREKFSG EIFAMGRHR YRGNVDSDD 
SDSSDDEPR PKRSAPGVS TTVNSPPVS AESARIARL 
EQLVENLNR RLLNRNFEH TSGQVGIKS DLIPEFAPG 
NPNFSTTKW LHKIDQLAL VNGWDERTT IYGMISRLK 
GLAKEWYDN LDPSAYDRS WDEWKRLLQ ATFPDHHDY 
ASTLLSCLI DGMTDVTLR NGARAGRYV TPESLYAEY 
LSTLRSEGD KRDTLAKQA PRERRRFLP SRVEAQKLY 
SSVRCYNCR NRGHVATKC PKPRIECKK CGRIGHVDA 
ECRRGNVVK ENMSKQALT TSVADASNN KNYYIDIKV 
NGKPTRAYI DSGAEPVLV KQSVARELG LMWQPSLHL 
IRGYGQGIT KVHGEVEVE LEVDLVKAN VKALIVEDS 
AQRVPVIVG QTFLNAGAN TIIANEEAV RVVDGNNES 
IQAFLGQLP TRKVALWAE EETVIPPQS IGCIRIKAK 
DDGWKGAYY VEGCQRPRP GFEHVVHRC VTCDGGLVT 
VRNLSHQDL VYRKAQIVA RAEPCEQER TATTVSVFS 
VGKVEVKSF QRWELLTQV NKNLDPAQF EQLLQLVNE 
FRDCFARNV AEIGATTVA EMKLTLTDD KPVIYRPYR 
LSHHERRIV RDIVNDLLG SGVIRESDS PYSSPILLV 
RKKDGQHRM CVDYRQLNS KTIKDRFPL PRVDEHLDK 
LNGAKFFTT LDLASGYYQ IPMATESIP KTAFVTPDG 
HYEFVRMPF GLANAPAVF QRAMNKVLG PLRFQTAFC 
YIDDLLIPS KDFETGLNN LRTVFQLLR QFGLTLKLS 
KCCFFGSQI EYLGHEISA EGIKPGETK IKAVTAFPK 
PTDVHKLRQ FLGLCGYFR KYVKDYATI ANSLTSLLK 
KGSAFVWEE AQERAFQTL KDILTSRPV LAIYDAEAE 
TELHTDASK VGIGGILLQ RQGDGSLRP VMFFSRQTT 
KEEQRYHSY ELETLAVVC SLKHYRVYL LGLQFKVIT 
DCNALRTTL TKRDLIPRI GRWWLLTSE FDFTVEYRP 
GSKMSHVDA LSRNPVLDP SEPSVEVLH INVNDDDWI 
LAVQMKDAR CALLKEILE KSPTNAEER AIHKEYKLK 
DGRVFKQTE SGLKWVVPK AVRRQILLA HHDGIGHLS 
NEKTLASVS KSYWFPSMR SKKFKTHCT DLNVKLVLT 
ATATPRANG QVERLNRTI LSQLAASSR EEEKWDDFV 
SRISWGINS TPNSTTGKS PYELLLGYT PRHANDSIL 
TNVVTEGVH DGDLPRTRA DVAQRVEKE QQKQKERYD 
KRRCAPKRF NAGDLVLVR TTRASNEGK SRKLLPKYS 
GPYRIQKVL DYDRYRGNV DSDDSDSSD DEPRPKRSA 
PGVSTTVNS PPVSAESAR IARLEQLVE NLNRRLLNR 
NFEHTSGQV GIKSDLIPE FAPGNPNFS TTKWLHKID 
QLALVNGWD ERTTIYGMI SRLKGLAKE WYDNLDPSA 
YDRSWDEWK LSCLIDGMT DVTLRNGAR AGRYVTPES 
LYAEYLSTL RSEGDKRDT LAKQAPRER RRFLPSRVE 
TQKLYSARN QELSARNVV ALDMWMLSR RGNLVKENM 
SKQALTTSV ADASNNKNY YIDIKVNGK PTRAYIDSG 
AEPVLVKQS VARELGLMW QPSLHLIRG YGQGITKVH 
GEVEVELEV DLVKANVKA LIVEDSAQR VPVIVGQTF 
LNAGANTII ANEEAVRVV DGNNESIQA FLGQLPTRK 
VALWAEEET VIPPQSIGC IRIKAKDDG KKFKTHCTD 
LNVKLVLTA TATPRANGQ VERLNRTIL SQLAASSRE 
EEKWDDFVS RISWGINST PNSTTGKSP YELLLGYTP 
RHANDSILT NVVTEGVHD GDLPRTRAD VAQRVEKEQ 
QKQKERYDK RRCAPKRFN AGDLVLVRT TRASNEGKS 
RKLLPKYSG PYRIQKVLD YDRYVVTDM PGATRSQKR 
YEGVHSVDK LRHYVVATT SGDETIGDE LRNHTLSIF 
IWVWLKRRE KFSGEIFAM GRHRYRGNV DSDDSDSSD 
DEPRPKRSA PGVSTTVNS PPVSAESAR IARLEQLVE 
NLNRRLLNR NFEHTSGQV GIKSGLIPE FAPGNPNFS 
TTKWLHKID QLALVNGWD ERTTIYGMI SRLKGLAKE 
WYDNLDPSA YDRSWDEWK RLLQATFPD HHDYASTLR 
KLVNRVKED GETWAQYYF GKLNLLQAC DITGTNAVS 
CLIDGITDV TLRNGARAG RYVTPESLY AEYLSTLRS 
EGDKRDTLA KQAPRERRR FLPSRVEAQ KLYSSVRCY 
NCRNRGHVA TKCPKPRIE CKKCGRIGH VDAECRRGN 
VVKENMSKQ ALTTSVADA SNNKNYYID IKVNGKPTR 
AYIDSGAEP VLVKQSVAR ELGLMWQPS LHLIRGYGQ 
GITKVHGEV EVELEVDLV KANVKALIV EDSAQRVPV 
IVGQTFLNA GANTIIANE EAVRVVDGN NESIQAFLG 
QLPTRKVAL WAEDETVIP PQSIGCIRI KAKDDGWKG 
AYYVEGCQR PRPGFEHVV HRCVTCDGG LVTVRNLSH 
QDLVYRKAQ IVARAEPCE QEWTATTLA ASSREEEKW 
DDFVSRISW GINSTPNST TGKSPYELL LGYTPRHAN 
DSILTNVVT EGVHDGDLP RTRADVAQR VEKEQQKQK 
ERYDKRRCA PKRFNAGDL VLVRTTRAS NEGKSRKLL 
PKYSGPYRI QKVLDYDRY VVTDMPGAT RSQKRYEGV 
HSVDKLRHY VVATTSGDE TIGDVSDE
    CDS sequence
    • >TC031587 RA
      atggggcgt cacaggtat cgaggaaat gtggactcg 
gacgactcg gactcaagt gacgatgag ccacggccc 
aaaaggtcc acgcctggg gtctccact acagtaaat 
tcaccccca gtttcggcc gagtccgct agaatcgcg 
agacttgag caacttgtg gaaaacttg aatcgacgt 
ttgctaaac cgcaatttt gagcacaca tcaggacaa 
gttggcatt aaaagtgac ctgattcct gaattcgct 
ccaggaaac cccaatttt agtaccact aagtggttg 
cataaaatt gaccagttg gctctcgtg aacggttgg 
gacgaacgt acaacgatt tatggcatg ataagtcga 
ctcaagggg ttggccaag gagtggtac gacaatctc 
gatccctca gcatacgac cggagctgg gatgaatgg 
aaacgtttg ttacaagcc accttcccc gatcaccac 
gattatgct tccacactt cgtaagcta gtgaatagg 
gtgaaggaa gacggtgaa acctgggca caatattat 
tttgggaaa ttaaatctg ttgcaggct tgtgacata 
acaggaacg aatgcagtc tcctgtctt attgatgga 
atcactgat gtgactctc agaaatggg gctagagca 
gggcgttac gttacaccc gaaagcttg tacgctgag 
tatttgtcg actctgaga tccgaaggt gacaagcgg 
gatacgctc gcaaagcag gcgcctcgg gaaaggagg 
aggttcctt ccaagtcga gtcgaagca caaaaactg 
tactcgtca gttcggtgt tacaattgt cgtaatcga 
ggacatgtt gcgacaaag tgcccgaaa ccaagaatt 
gagtgcaag aaatgtggt cgcattgga catgtggat 
gctgagtgc cgacgtggt aacgtagta aaagaaaac 
atgtctaag caggcacta acgacaagt gtagctgac 
gcaagtaat aataagaat tattacata gacattaag 
gtcaatggt aagccgacg cgagcttac attgactca 
ggagctgaa ccggtcctt gtcaagcaa agtgttgct 
agagaattg ggactgatg tggcagcca agtttacat 
ttgattcgt ggttatggc cagggaatc accaaagtg 
catggggaa gtcgaagta gagctagaa gtggatctt 
gtcaaggca aacgtaaaa gctttgatc gtcgaagac 
agtgcacaa cgtgtgcct gtcatagtg ggacagacc 
ttcctgaat gcgggtgct aatacgatt atagcaaat 
gaggaagct gtgagagta gttgacgga aacaacgaa 
tcgatccaa gcatttcta ggccaactt ccgacacga 
aaagttgca ctttgggcg gaagaggaa acagtgatc 
cctcctcaa tccattggt tgcatacga atcaaggca 
aaagacgat gggtggaaa ggagcttat tacgttgaa 
tggtgtcaa cgtccgaga ccagggttc gaacacgtt 
gttcatcgg tgtgtcaca tgtgatgga ggcctagtg 
accgttcgc aatctatcg caccaagat ctggtctat 
cgaaaggcg caaatcgtt gcgagagct gagccttgc 
gagcaggaa aggacagct acgacggtg agtgtgttt 
tctgtgggg aaggttgag gtaaagagt tttcaacgg 
tgggaactt cttacacaa gtcaataag aatctagac 
ccggctcag ttcgagcaa ttgttacaa ctcgttaac 
gaatttcga gattgtttt gcacgaaat gtagcggaa 
ataggtgcc acaactgcg gcagaaatg aagctaact 
ttgactgat gataaacct gtaatttat cgtccttat 
cggttatcg catcacgaa agaagaatt gttcgagat 
atagtaaat gacttgtta ggtagcgga gtcatacga 
gaatcagac tcgccatat tcgagtccc atactgttg 
gtgcggaag aaagatggg caacaccgt atgtgtgtt 
gactatcga caattaaat tcgaaaaca atcaaagat 
cgctttccg ttaccacga gtagatgaa catttggat 
aaactaaaa ggtgcaaag tttttcacc acactagat 
ctcgcgagt gggtacttt cagatccca atggccacc 
gaatcgatt ccaaagaca gcatttgtt acgcctgat 
gggcattgc gagtttgtc cgcatgcct tttggtcta 
gccaacgct ccggccgtg tttcagcga gctatgaat 
aaggtgttg ggtccatta cagtttcag accgctttt 
tgttacata gatgatctt ctgatacct tccaaagac 
tttgaaaca ggtcttaac aatttacaa acggtgttt 
caattgctt cgacagttc ggattgact ctaaagctg 
agtaaatat tgcttcttt ggaagtcaa atagagtat 
ttagggcat gagatcagt gcggaaggc attaagcca 
ggcgagaca aaaatcaaa gcggtgaca gcttttccc 
aaaccaaca gacgtacac aaacttagg caattctta 
ggtttgtgt gggtacttt cgaaagtac gtgaaagat 
tacgcaaca atagcaaac agtttgaca agtctccta 
aagaatggc agtgcattt gttttggaa gaagtacaa 
gaacgggct tttcagact ttaaaagac atcttgacg 
agcagacca gttcttgca atttacgac gcggaagct 
gaaacggag ttacacact gacgcttct aaagttgga 
attggtggc attctgttg caacgacaa ggtgatgga 
tctttgcgt ccagttatg tttttcagc cgacagacg 
accaaagag gaacaaagg taccactcg tacgagcta 
gaaacgcta gcggtggtc tgttctctc aaacattat 
cgagtatac ttgttgggg cttcaattc aaggtgatc 
acagactgt aatgcttta cgaacaacc cttaccaag 
agagatttg attccacga attgggaga tggtggttg 
ttgacttct gagttcgac tttactgta gaatacaga 
cctggcagt aaaatgagt cacgttgac gctttgagt 
agaaatacg gtattagat ccttcggag ccttcagtg 
gaagttttg catattaat gttaacgat gatgattgg 
attttagct gttcaaatg aaggatgcg agatgcgca 
ttgttgaag gaaatattg gaaaaatca ccgactaac 
gctgaagag cgtgccatt cacaaagag tataaacta 
aaagatggt cgagtattc aaacagact gagtcagga 
ttaaaatgg gtcgtacca aaagcggta agacgccag 
attttatta gcacaccac gatggaatt ggccattta 
tcgaatgag aaaactttg gcatctgtg tcgaaatct 
tattggttt ccttcaatg cggagcaaa aagtttaag 
acacattgt accgattta aacgttaaa ttagtacta 
accgcgact gccacacca cgggctaat ggccaggtg 
gagcgatta aatcgtacg attttatct cagcttgct 
gctagctcg agagaagaa gagaaatgg gatgacttt 
gtgagtaga attagttgg ggtattaat tctacgccg 
aattcgact acgggcaaa agtccatac gaattgttg 
ttggggtat acacctcga catgctaat gactcaatc 
ctcaccaac gtggtgaca gagggtgtt catgatggc 
gatttgcca cggacacga gcagacgtt gctcagcgt 
gtcgaaaag gaacagcag aagcaaaag gagcgttat 
gacaagcga cgctgtgcc ccaaagaga tttaatgca 
ggtgatctc gtgttggta cgcaccacg cgagtttca 
aacgagggc aaaagtcgg aagctgtta cctaaatat 
tcgggaccg taccgaatt cagcaagtg ctcgactac 
gataggtat gtcgtgacg gacatgccg ggggcgacg 
cgttcccaa aaacgatat gagggtgtt cactcagtg 
gacaaattg agacattac gttgtcgcc acgacgagt 
ggagacgaa acaataggc gatgagtta cgcaaccac 
acgctgtca attttcatt tgggtgtgg ctcaaaagg 
cgcgaaaag ttttctggt gagattttc gcaatgggg 
cgtcacagg taccgagga aatgtggac tcggacgac 
tcggactca agtgacgat gagccacgg ccgaaaagg 
tccgcgcct ggggtctcc actacagta aattcaccc 
ccagtttcg gccgagtcc gctagaatc gcgagactt 
gagcaactt gtggaaaac ttgaatcga cgtttgcta 
aaccgcaat tttgagcac acatcagga caagttggc 
attaaaagt gacctgatt cctgaattc gctccagga 
aaccccaat tttagtacc actaagtgg ttgcataaa 
attgaccag ttggctctc gtgaacggt tgggacgaa 
cgtacaacg atttacggc atgataagt cgactcaag 
gggttggcc aaggagtgg tacgacaat ctcgatccc 
tcagcatac gaccggagc tgggatgaa tggaaacgt 
ttgttacaa gccaccttc cccgatcac cacgattat 
gcttccaca cttctctcc tgtcttatt gatggaatg 
actgacgtg actctcaga aatggggct agagcaggg 
cgttacgtt acacccgaa agcttgtac gctgagtat 
ttgtcgact ctgagatcc gaaggtgac aagcgggat 
acgctcgca aagcaggcg cctcgggaa aggaggagg 
ttccttcca agtcgagtt gaagcacaa aaactgtat 
tcgtcagtt cggtgttac aattgtcgt aatcgagga 
catgttgcg acaaagtgc ccgaaacca agaattgag 
tgcaagaaa tgtggtcgc attggacat gtggatgct 
gagtgccga cgtggtaac gtagtaaaa gaaaacatg 
tctaagcag gcactaacg acaagtgta gctgacgca 
agtaataat aagaattat tacatagac attaaggtc 
aatggtaag ccgacgcga gcttacatt gactcagga 
gctgaaccg gtcctggtc aagcaaagt gttgctaga 
gaattggga ctgatgtgg cagccaagt ttacatttg 
attcgtggt tatggccag ggaatcacc aaagtgcat 
ggggaagtc gaagtagag ctagaagtg gatcttgtc 
aaggcaaac gtaaaagct ttgatcgtc gaagacagt 
gcacaacgt gtgcctgtc atagtggga cagaccttc 
ctgaatgcg ggtgctaat acgattata gcaaatgag 
gaagctgtg agagtagtt gacggaaac aacgaatcg 
atccaagca tttctaggc caacttccg acacgaaaa 
gttgcactt tgggcggaa gaggaaaca gtgatccct 
cctcaatcc attggttgc atacgaatc aaggcaaaa 
gacgatggg tggaaagga gcttattac gttgaaggg 
tgtcaacgt ccgagacca gggttcgaa cacgttgtt 
catcggtgt gtcacatgt gatggaggc ctagtgacc 
gttcgcaat ctatcgcac caagatctg gtctatcga 
aaggcgcaa atcgttgcg agagctgag ccttgcgag 
caggaaagg acagctacg acggtgagt gtgttttct 
gtggggaag gttgaggta aagagtttt caacggtgg 
gaacttctt acacaagtc aataagaat ctagacccg 
gctcagttc gagcaattg ttacaactc gttaacgaa 
tttcgagat tgttttgca cgaaatgta gcggaaata 
ggtgccaca actgtggca gaaatgaag ctaactttg 
actgatgat aaacctgta atttatcgt ccttatcgg 
ttatcgcat cacgaaaga agaattgtt cgagatata 
gtaaatgac ttgttaggt agcggagtc atacgagaa 
tcagactcg ccatattcg agtcccata ctgttggtg 
cggaagaaa gatgggcaa caccgtatg tgtgttgac 
tatcggcaa ttaaattcg aaaacaatc aaagatcgc 
tttccgtta ccacgagta gatgaacat ttggataaa 
ctaaacggt gcaaagttt ttcaccaca ctagatctc 
gcgagtggg tactatcag atcccaatg gccaccgaa 
tcgattcca aagacagca tttgttacg cctgatggg 
cattacgag tttgtccgc atgcctttt ggtctagcc 
aacgctccg gcagtgttt cagcgagct atgaataag 
gtgttgggt ccattacgg tttcagacc gctttttgt 
tacatagat gatcttctg ataccttcc aaagacttt 
gaaacaggt cttaacaat ttacgaacg gtgtttcaa 
ttgcttcga cagttcgga ttgactcta aagctgagt 
aaatgttgc ttctttgga agtcaaata gagtattta 
gggcatgag atcagtgcg gaaggcatt aagccaggc 
gagacaaaa atcaaagcg gtgacagct tttcccaaa 
ccaacagac gtacacaaa cttaggcaa ttcttaggt 
ttgtgtggg tactttcga aagtacgtg aaagattac 
gcaacaata gcaaacagt ttgacgagt ctcctaaag 
aaaggcagt gcatttgtt tgggaagaa gcacaagag 
cgggctttt cagacttta aaagacatc ttgacgagc 
agaccagtt cttgcaatt tacgacgcg gaagctgaa 
acggagtta cacactgac gcttctaaa gttggaatt 
ggtggcatt ctgttgcaa cgacaaggt gatggatct 
ttgcgtcca gttatgttt ttcagccga cagacgacc 
aaagaggaa caaaggtac cactcgtac gagctagaa 
acgctagcg gtggtctgt tctctcaaa cattatcga 
gtatacttg ttggggctt caattcaag gtgatcaca 
gactgtaat gctttacga acaaccctt accaagaga 
gatttgatt ccacgaatt gggagatgg tggttgttg 
acttctgag ttcgacttt actgtagaa tacagacct 
ggcagtaaa atgagtcac gttgacgct ttgagtaga 
aatccggta ttagatcct tcggagcct tcagtggaa 
gtcttgcat attaatgtt aacgatgat gattggatt 
ttagctgtt caaatgaag gatgcgaga tgcgcattg 
ttgaaggaa atattggaa aaatcaccg acgaacgct 
gaagagcgt gccattcac aaagagtat aaactaaaa 
gatggtcga gtattcaaa cagactgag tcaggatta 
aaatgggtc gtaccaaaa gcggtaaga cgccagatt 
ttattagca caccacgat ggaattggc catttatcg 
aatgagaaa actttggca tctgtgtcg aaatcttat 
tggtttcct tcaatgcgg agcaaaaag tttaagaca 
cattgtacc gatttaaac gttaaatta gtactaacc 
gcgactgct acaccacgg gctaacggc caggtggag 
cgattgaat cgtacgatt ttatctcag cttgctgct 
agctcgaga gaagaagag aaatgggat gactttgtg 
agtagaatt agttggggt attaattct acgccgaat 
tcgactacg ggcaaaagt ccatacgaa ttgttgttg 
gggtataca cctcgacat gctaatgac tcaatcctc 
accaacgtg gtgacagag ggtgttcat gatggtgat 
ttgccacgg acacgagca gacgttgct cagcgtgtc 
gaaaaggaa cagcagaag caaaaagag cgttatgac 
aagcgacgc tgtgcccca aagagattt aatgcaggt 
gatctcgtg ttggtacgc accacgcga gcttcaaac 
gagggcaaa agtcggaag ctgttacct aaatattcg 
ggaccgtac cgaattcag aaagtgctc gactacgat 
aggtaccga ggaaatgtg gactcggac gactcggac 
tcaagtgac gatgagcca cggccgaaa aggtccgcg 
cctggggtc tccactaca gtaaattca cccccagtt 
tcggccgag tccgctaga atcgcgaga cttgagcaa 
cttgtggaa aacttgaat cgacgtttg ctaaaccgt 
aattttgag cacacatca ggacaagtt ggcattaaa 
agtgacttg attcctgaa ttcgctcca ggaaacccc 
aattttagt accactaag tggttgcat aaaattgac 
cagttggct ctcgtgaac ggttgggac gaacgtaca 
acgatttat ggcatgata agtcgactc aaggggttg 
gccaaggag tggtacgac aatctcgat ccctcagca 
tatgaccgg agctgggat gaatggaaa ctctcctgt 
cttattgat ggaatgact gacgtgact ctcagaaat 
ggggctaga gcagggcgt tacgttaca cccgaaagc 
ttgtacgct gagtatttg tcgactctg agatccgaa 
ggtgacaag cgggatacg ctcgcaaag caggcgcct 
cgggaaagg aggaggttc cttccaagt cgagtcgaa 
acacaaaaa ctgtattct gcccgaaac caagaattg 
agtgcaaga aatgtggtc gcattggac atgtggatg 
cttagccga cgtggtaac ttagtaaaa gaaaacatg 
tctaagcag gcactaacg acaagtgta gctgacgca 
agtaataat aagaattat tacatagac attaaggtc 
aatggcaag ccgacgcga gcttacatt gactcagga 
gctgaaccg gtccttgtc aagcaaagt gttgctaga 
gaattggga ctgatgtgg cagccaagt ttacatttg 
attcgtggt tatggccag ggaatcacc aaagtgcat 
ggggaagtc gaagtagag ctagaagtg gatcttgtc 
aaggcaaac gtaaaagct ttgatcgtc gaagacagt 
gcacaacgt gtgcctgtc atagtggga cagaccttc 
ctgaatgcg ggtgctaat acgattata gcaaatgag 
gaagctgtg agagtagtt gacggaaac aacgaatcg 
atccaagca tttctaggc caacttccg acacgaaaa 
gttgcactt tgggcggaa gaggaaaca gtgatccct 
cctcaatcc attggttgc atacgaatc aaggcaaaa 
gacgatggc aaaaagttt aagacacat tgtaccgat 
ttaaacgtt aaattagta ctaaccgcg actgctaca 
ccacgggct aacggccag gtggagcga ttgaatcgt 
acgatttta tctcagctt gctgctagc tcgagagaa 
gaagagaaa tgggatgac tttgtgagt agaattagt 
tggggtatt aattctacg ccgaattcg actacgggc 
aaaagtcca tacgaattg ttgttgggg tatacacct 
cgacatgct aatgactca atcctcacc aacgtggtg 
acagagggt gttcatgat ggtgatttg ccacggaca 
cgagcagac gttgctcag cgtgtcgaa aaggaacag 
cagaagcaa aaagagcgt tatgacaag cgacgctgt 
gccccaaag agatttaat gcaggtgat ctcgtgttg 
gtacgcacc acgcgagct tcaaacgag ggcaaaagt 
cggaagctg ttacctaaa tattcggga ccgtaccga 
attcagaaa gtgctcgac tacgatagg tacgtcgtg 
acggacatg ccgggggcg acgcgttcc caaaaacga 
tatgagggt gttcactca gtggacaaa ttgagacat 
tacgttgtc gccacgacg agtggagac gaaacaata 
ggcgatgag ttacgcaac cacacgctg tcaattttc 
atttgggtg tggctcaaa aggcgcgaa aagttttct 
ggtgagatt ttcgcaatg gggcgtcac aggtaccga 
ggaaatgtg gactcggac gactcggac tcaagtgac 
gatgagcca cggccgaaa aggtccgcg cctggggtc 
tccactaca gtaaattca cccccagtt tcggccgag 
tccgctaga atcgcgaga cttgagcaa cttgtggaa 
aacttgaat cgacgtttg ctaaaccgc aattttgag 
cacacatca ggacaagtt ggcattaaa agtggcctg 
attcctgaa ttcgctcca ggaaacccc aattttagt 
accactaag tggttgcat aaaattgac cagttggct 
ctcgtgaac ggttgggac gaacgtaca acgatttac 
ggcatgata agtcgactc aaggggttg gccaaggag 
tggtacgac aatctcgat ccctcagca tacgaccgg 
agctgggat gaatggaaa cgtttgtta caagccacc 
ttccccgat caccacgat tatgcttcc acacttcgt 
aagctagtg aatagggtg aaggaagat ggggaaacc 
tgggcacaa tattatttt gggaaatta aatctgttg 
caggcttgt gacataaca ggaacgaat gcagtctcc 
tgtcttatt gatggaatc actgacgtg actctcaga 
aatggggct agagcaggg cgttacgtt acacccgaa 
agcttgtac gctgagtat ttgtcgact ctgagatcc 
gaaggtgac aagcgggat acgctcgca aagcaggcg 
cctcgggaa aggaggagg ttccttcca agtcgagtc 
gaagcacaa aaactgtat tcgtcagtt cggtgttac 
aattgtcgt aatcgagga catgttgcg acaaagtgc 
ccgaaacca agaattgag tgcaagaaa tgtggtcgc 
attggacat gtggatgct gagtgccga cgtggtaac 
gtagtaaaa gaaaacatg tctaagcag gcactaacg 
acaagtgta gctgacgca agtaataat aagaattat 
tacatagac attaaggtt aatggtaag ccgacgcga 
gcttacatt gactcagga gctgaaccg gtccttgtc 
aagcaaagt gttgctaga gaattggga ctgatgtgg 
cagccaagt ttacatttg attcgtggt tatggccag 
ggaatcacc aaagtgcat ggggaagtc gaagtagag 
ctagaagtg gatcttgtc aaggcaaac gtaaaagct 
ttgatcgtc gaagacagt gcacaacgt gtgcctgtc 
atagtggga cagaccttc ctgaatgcg ggtgctaat 
acgattata gcaaatgaa gaagctgtg agagtagtt 
gacggaaac aacgaatcg atccaagca tttctaggc 
caacttccg acacgaaaa gttgcactt tgggcggaa 
gacgaaaca gtgatccct cctcaatcc attggttgc 
atacgaatc aaggcaaaa gacgatggg tggaaagga 
gcttattac gttgaaggg tgtcaacgt ccgagacca 
gggttcgaa cacgttgtt catcggtgt gtcacatgt 
gatggaggc ctagtgacc gttcgcaat ctatcgcac 
caagatctg gtctatcga aaggcgcaa atcgttgcg 
agagctgag ccttgcgag caggaatgg acagctacg 
acgcttgct gctagctcg agagaagaa gagaaatgg 
gatgacttt gtgagtaga attagttgg ggtattaat 
tctacgccg aattcgact acgggcaaa agtccatac 
gaattgttg ttggggtat acacctcga catgctaat 
gactcaatc ctcaccaac gtggtgaca gagggtgtt 
catgatggt gatttgcca cggacacga gcagacgtt 
gctcagcgt gtcgaaaag gaacagcag aagcaaaaa 
gagcgttat gacaagcga cgctgtgcc ccaaagaga 
tttaatgca ggtgatctc gtgttggta cgcaccacg 
cgagcttca aacgagggc aaaagtcgg aagctgtta 
cctaaatat tcgggaccg taccgaatt cagaaagtg 
ctcgactac gataggtac gtcgtgacg gacatgccg 
ggggcgacg cgttcccaa aaacgatat gagggtgtt 
cactcagtg gacaaattg agacattac gttgtcgcc 
acgacgagt ggagacgaa acaataggc gatgtcagt 
gacgaatga
    mRNA sequence
    • >TC031587 RA
      agattttta aagttatta taaatccaa acgactgtg 
atatattac aactgtaga tttaaatag tttttgagt 
tacgcaacc acacgctgt caattttca tttgggtgt 
ggctcaaaa ggcgcgaaa agttttcta atgagattt 
tcgcaatgg ggcgtcaca ggtatcgag gaaatgtgg 
actcggacg actcggact caagtgacg atgagccac 
ggcccaaaa ggtccacgc ctggggtct ccactacag 
taaattcac ccccagttt cggccgagt ccgctagaa 
tcgcgagac ttgagcaac ttgtggaaa acttgaatc 
gacgtttgc taaaccgca attttgagc acacatcag 
gacaagttg gcattaaaa gtgacctga ttcctgaat 
tcgctccag gaaacccca attttagta ccactaagt 
ggttgcata aaattgacc agttggctc tcgtgaacg 
gttgggacg aacgtacaa cgatttatg gcatgataa 
gtcgactca aggggttgg ccaaggagt ggtacgaca 
atctcgatc cctcagcat acgaccgga gctgggatg 
aatggaaac gtttgttac aagccacct tccccgatc 
accacgatt atgcttcca cacttcgta agctagtga 
atagggtga aggaagacg gtgaaacct gggcacaat 
attattttg ggaaattaa atctgttgc aggcttgtg 
acataacag gaacgaatg cagtctcct gtcttattg 
atggaatca ctgatgtga ctctcagaa atggggcta 
gagcagggc gttacgtta cacccgaaa gcttgtacg 
ctgagtatt tgtcgactc tgagatccg aaggtgaca 
agcgggata cgctcgcaa agcaggcgc ctcgggaaa 
ggaggaggt tccttccaa gtcgagtcg aagcacaaa 
aactgtact cgtcagttc ggtgttaca attgtcgta 
atcgaggac atgttgcga caaagtgcc cgaaaccaa 
gaattgagt gcaagaaat gtggtcgca ttggacatg 
tggatgctg agtgccgac gtggtaacg tagtaaaag 
aaaacatgt ctaagcagg cactaacga caagtgtag 
ctgacgcaa gtaataata agaattatt acatagaca 
ttaaggtca atggtaagc cgacgcgag cttacattg 
actcaggag ctgaaccgg tccttgtca agcaaagtg 
ttgctagag aattgggac tgatgtggc agccaagtt 
tacatttga ttcgtggtt atggccagg gaatcacca 
aagtgcatg gggaagtcg aagtagagc tagaagtgg 
atcttgtca aggcaaacg taaaagctt tgatcgtcg 
aagacagtg cacaacgtg tgcctgtca tagtgggac 
agaccttcc tgaatgcgg gtgctaata cgattatag 
caaatgagg aagctgtga gagtagttg acggaaaca 
acgaatcga tccaagcat ttctaggcc aacttccga 
cacgaaaag ttgcacttt gggcggaag aggaaacag 
tgatccctc ctcaatcca ttggttgca tacgaatca 
aggcaaaag acgatgggt ggaaaggag cttattacg 
ttgaatggt gtcaacgtc cgagaccag ggttcgaac 
acgttgttc atcggtgtg tcacatgtg atggaggcc 
tagtgaccg ttcgcaatc tatcgcacc aagatctgg 
tctatcgaa aggcgcaaa tcgttgcga gagctgagc 
cttgcgagc aggaaagga cagctacga cggtgagtg 
tgttttctg tggggaagg ttgaggtaa agagttttc 
aacggtggg aacttctta cacaagtca ataagaatc 
tagacccgg ctcagttcg agcaattgt tacaactcg 
ttaacgaat ttcgagatt gttttgcac gaaatgtag 
cggaaatag gtgccacaa ctgcggcag aaatgaagc 
taactttga ctgatgata aacctgtaa tttatcgtc 
cttatcggt tatcgcatc acgaaagaa gaattgttc 
gagatatag taaatgact tgttaggta gcggagtca 
tacgagaat cagactcgc catattcga gtcccatac 
tgttggtgc ggaagaaag atgggcaac accgtatgt 
gtgttgact atcgacaat taaattcga aaacaatca 
aagatcgct ttccgttac cacgagtag atgaacatt 
tggataaac taaaaggtg caaagtttt tcaccacac 
tagatctcg cgagtgggt actttcaga tcccaatgg 
ccaccgaat cgattccaa agacagcat ttgttacgc 
ctgatgggc attgcgagt ttgtccgca tgccttttg 
gtctagcca acgctccgg ccgtgtttc agcgagcta 
tgaataagg tgttgggtc cattacagt ttcagaccg 
ctttttgtt acatagatg atcttctga taccttcca 
aagactttg aaacaggtc ttaacaatt tacaaacgg 
tgtttcaat tgcttcgac agttcggat tgactctaa 
agctgagta aatattgct tctttggaa gtcaaatag 
agtatttag ggcatgaga tcagtgcgg aaggcatta 
agccaggcg agacaaaaa tcaaagcgg tgacagctt 
ttcccaaac caacagacg tacacaaac ttaggcaat 
tcttaggtt tgtgtgggt actttcgaa agtacgtga 
aagattacg caacaatag caaacagtt tgacaagtc 
tcctaaaga atggcagtg catttgttt tggaagaag 
tacaagaac gggcttttc agactttaa aagacatct 
tgacgagca gaccagttc ttgcaattt acgacgcgg 
aagctgaaa cggagttac acactgacg cttctaaag 
ttggaattg gtggcattc tgttgcaac gacaaggtg 
atggatctt tgcgtccag ttatgtttt tcagccgac 
agacgacca aagaggaac aaaggtacc actcgtacg 
agctagaaa cgctagcgg tggtctgtt ctctcaaac 
attatcgag tatacttgt tggggcttc aattcaagg 
tgatcacag actgtaatg ctttacgaa caaccctta 
ccaagagag atttgattc cacgaattg ggagatggt 
ggttgttga cttctgagt tcgacttta ctgtagaat 
acagacctg gcagtaaaa tgagtcacg ttgacgctt 
tgagtagaa atacggtat tagatcctt cggagcctt 
cagtggaag ttttgcata ttaatgtta acgatgatg 
attggattt tagctgttc aaatgaagg atgcgagat 
gcgcattgt tgaaggaaa tattggaaa aatcaccga 
ctaacgctg aagagcgtg ccattcaca aagagtata 
aactaaaag atggtcgag tattcaaac agactgagt 
caggattaa aatgggtcg taccaaaag cggtaagac 
gccagattt tattagcac accacgatg gaattggcc 
atttatcga atgagaaaa ctttggcat ctgtgtcga 
aatcttatt ggtttcctt caatgcgga gcaaaaagt 
ttaagacac attgtaccg atttaaacg ttaaattag 
tactaaccg cgactgcca caccacggg ctaatggcc 
aggtggagc gattaaatc gtacgattt tatctcagc 
ttgctgcta gctcgagag aagaagaga aatgggatg 
actttgtga gtagaatta gttggggta ttaattcta 
cgccgaatt cgactacgg gcaaaagtc catacgaat 
tgttgttgg ggtatacac ctcgacatg ctaatgact 
caatcctca ccaacgtgg tgacagagg gtgttcatg 
atggcgatt tgccacgga cacgagcag acgttgctc 
agcgtgtcg aaaaggaac agcagaagc aaaaggagc 
gttatgaca agcgacgct gtgccccaa agagattta 
atgcaggtg atctcgtgt tggtacgca ccacgcgag 
tttcaaacg agggcaaaa gtcggaagc tgttaccta 
aatattcgg gaccgtacc gaattcagc aagtgctcg 
actacgata ggtatgtcg tgacggaca tgccggggg 
cgacgcgtt cccaaaaac gatatgagg gtgttcact 
cagtggaca aattgagac attacgttg tcgccacga 
cgagtggag acgaaacaa taggcgatg agttacgca 
accacacgc tgtcaattt tcatttggg tgtggctca 
aaaggcgcg aaaagtttt ctggtgaga ttttcgcaa 
tggggcgtc acaggtacc gaggaaatg tggactcgg 
acgactcgg actcaagtg acgatgagc cacggccga 
aaaggtccg cgcctgggg tctccacta cagtaaatt 
cacccccag tttcggccg agtccgcta gaatcgcga 
gacttgagc aacttgtgg aaaacttga atcgacgtt 
tgctaaacc gcaattttg agcacacat caggacaag 
ttggcatta aaagtgacc tgattcctg aattcgctc 
caggaaacc ccaatttta gtaccacta agtggttgc 
ataaaattg accagttgg ctctcgtga acggttggg 
acgaacgta caacgattt acggcatga taagtcgac 
tcaaggggt tggccaagg agtggtacg acaatctcg 
atccctcag catacgacc ggagctggg atgaatgga 
aacgtttgt tacaagcca ccttccccg atcaccacg 
attatgctt ccacacttc tctcctgtc ttattgatg 
gaatgactg acgtgactc tcagaaatg gggctagag 
cagggcgtt acgttacac ccgaaagct tgtacgctg 
agtatttgt cgactctga gatccgaag gtgacaagc 
gggatacgc tcgcaaagc aggcgcctc gggaaagga 
ggaggttcc ttccaagtc gagttgaag cacaaaaac 
tgtattcgt cagttcggt gttacaatt gtcgtaatc 
gaggacatg ttgcgacaa agtgcccga aaccaagaa 
ttgagtgca agaaatgtg gtcgcattg gacatgtgg 
atgctgagt gccgacgtg gtaacgtag taaaagaaa 
acatgtcta agcaggcac taacgacaa gtgtagctg 
acgcaagta ataataaga attattaca tagacatta 
aggtcaatg gtaagccga cgcgagctt acattgact 
caggagctg aaccggtcc tggtcaagc aaagtgttg 
ctagagaat tgggactga tgtggcagc caagtttac 
atttgattc gtggttatg gccagggaa tcaccaaag 
tgcatgggg aagtcgaag tagagctag aagtggatc 
ttgtcaagg caaacgtaa aagctttga tcgtcgaag 
acagtgcac aacgtgtgc ctgtcatag tgggacaga 
ccttcctga atgcgggtg ctaatacga ttatagcaa 
atgaggaag ctgtgagag tagttgacg gaaacaacg 
aatcgatcc aagcatttc taggccaac ttccgacac 
gaaaagttg cactttggg cggaagagg aaacagtga 
tccctcctc aatccattg gttgcatac gaatcaagg 
caaaagacg atgggtgga aaggagctt attacgttg 
aagggtgtc aacgtccga gaccagggt tcgaacacg 
ttgttcatc ggtgtgtca catgtgatg gaggcctag 
tgaccgttc gcaatctat cgcaccaag atctggtct 
atcgaaagg cgcaaatcg ttgcgagag ctgagcctt 
gcgagcagg aaaggacag ctacgacgg tgagtgtgt 
tttctgtgg ggaaggttg aggtaaaga gttttcaac 
ggtgggaac ttcttacac aagtcaata agaatctag 
acccggctc agttcgagc aattgttac aactcgtta 
acgaatttc gagattgtt ttgcacgaa atgtagcgg 
aaataggtg ccacaactg tggcagaaa tgaagctaa 
ctttgactg atgataaac ctgtaattt atcgtcctt 
atcggttat cgcatcacg aaagaagaa ttgttcgag 
atatagtaa atgacttgt taggtagcg gagtcatac 
gagaatcag actcgccat attcgagtc ccatactgt 
tggtgcgga agaaagatg ggcaacacc gtatgtgtg 
ttgactatc ggcaattaa attcgaaaa caatcaaag 
atcgctttc cgttaccac gagtagatg aacatttgg 
ataaactaa acggtgcaa agtttttca ccacactag 
atctcgcga gtgggtact atcagatcc caatggcca 
ccgaatcga ttccaaaga cagcatttg ttacgcctg 
atgggcatt acgagtttg tccgcatgc cttttggtc 
tagccaacg ctccggcag tgtttcagc gagctatga 
ataaggtgt tgggtccat tacggtttc agaccgctt 
tttgttaca tagatgatc ttctgatac cttccaaag 
actttgaaa caggtctta acaatttac gaacggtgt 
ttcaattgc ttcgacagt tcggattga ctctaaagc 
tgagtaaat gttgcttct ttggaagtc aaatagagt 
atttagggc atgagatca gtgcggaag gcattaagc 
caggcgaga caaaaatca aagcggtga cagcttttc 
ccaaaccaa cagacgtac acaaactta ggcaattct 
taggtttgt gtgggtact ttcgaaagt acgtgaaag 
attacgcaa caatagcaa acagtttga cgagtctcc 
taaagaaag gcagtgcat ttgtttggg aagaagcac 
aagagcggg cttttcaga ctttaaaag acatcttga 
cgagcagac cagttcttg caatttacg acgcggaag 
ctgaaacgg agttacaca ctgacgctt ctaaagttg 
gaattggtg gcattctgt tgcaacgac aaggtgatg 
gatctttgc gtccagtta tgtttttca gccgacaga 
cgaccaaag aggaacaaa ggtaccact cgtacgagc 
tagaaacgc tagcggtgg tctgttctc tcaaacatt 
atcgagtat acttgttgg ggcttcaat tcaaggtga 
tcacagact gtaatgctt tacgaacaa cccttacca 
agagagatt tgattccac gaattggga gatggtggt 
tgttgactt ctgagttcg actttactg tagaataca 
gacctggca gtaaaatga gtcacgttg acgctttga 
gtagaaatc cggtattag atccttcgg agccttcag 
tggaagtct tgcatatta atgttaacg atgatgatt 
ggattttag ctgttcaaa tgaaggatg cgagatgcg 
cattgttga aggaaatat tggaaaaat caccgacga 
acgctgaag agcgtgcca ttcacaaag agtataaac 
taaaagatg gtcgagtat tcaaacaga ctgagtcag 
gattaaaat gggtcgtac caaaagcgg taagacgcc 
agattttat tagcacacc acgatggaa ttggccatt 
tatcgaatg agaaaactt tggcatctg tgtcgaaat 
cttattggt ttccttcaa tgcggagca aaaagttta 
agacacatt gtaccgatt taaacgtta aattagtac 
taaccgcga ctgctacac cacgggcta acggccagg 
tggagcgat tgaatcgta cgattttat ctcagcttg 
ctgctagct cgagagaag aagagaaat gggatgact 
ttgtgagta gaattagtt ggggtatta attctacgc 
cgaattcga ctacgggca aaagtccat acgaattgt 
tgttggggt atacacctc gacatgcta atgactcaa 
tcctcacca acgtggtga cagagggtg ttcatgatg 
gtgatttgc cacggacac gagcagacg ttgctcagc 
gtgtcgaaa aggaacagc agaagcaaa aagagcgtt 
atgacaagc gacgctgtg ccccaaaga gatttaatg 
caggtgatc tcgtgttgg tacgcacca cgcgagctt 
caaacgagg gcaaaagtc ggaagctgt tacctaaat 
attcgggac cgtaccgaa ttcagaaag tgctcgact 
acgataggt accgaggaa atgtggact cggacgact 
cggactcaa gtgacgatg agccacggc cgaaaaggt 
ccgcgcctg gggtctcca ctacagtaa attcacccc 
cagtttcgg ccgagtccg ctagaatcg cgagacttg 
agcaacttg tggaaaact tgaatcgac gtttgctaa 
accgtaatt ttgagcaca catcaggac aagttggca 
ttaaaagtg acttgattc ctgaattcg ctccaggaa 
accccaatt ttagtacca ctaagtggt tgcataaaa 
ttgaccagt tggctctcg tgaacggtt gggacgaac 
gtacaacga tttatggca tgataagtc gactcaagg 
ggttggcca aggagtggt acgacaatc tcgatccct 
cagcatatg accggagct gggatgaat ggaaactct 
cctgtctta ttgatggaa tgactgacg tgactctca 
gaaatgggg ctagagcag ggcgttacg ttacacccg 
aaagcttgt acgctgagt atttgtcga ctctgagat 
ccgaaggtg acaagcggg atacgctcg caaagcagg 
cgcctcggg aaaggagga ggttccttc caagtcgag 
tcgaaacac aaaaactgt attctgccc gaaaccaag 
aattgagtg caagaaatg tggtcgcat tggacatgt 
ggatgctta gccgacgtg gtaacttag taaaagaaa 
acatgtcta agcaggcac taacgacaa gtgtagctg 
acgcaagta ataataaga attattaca tagacatta 
aggtcaatg gcaagccga cgcgagctt acattgact 
caggagctg aaccggtcc ttgtcaagc aaagtgttg 
ctagagaat tgggactga tgtggcagc caagtttac 
atttgattc gtggttatg gccagggaa tcaccaaag 
tgcatgggg aagtcgaag tagagctag aagtggatc 
ttgtcaagg caaacgtaa aagctttga tcgtcgaag 
acagtgcac aacgtgtgc ctgtcatag tgggacaga 
ccttcctga atgcgggtg ctaatacga ttatagcaa 
atgaggaag ctgtgagag tagttgacg gaaacaacg 
aatcgatcc aagcatttc taggccaac ttccgacac 
gaaaagttg cactttggg cggaagagg aaacagtga 
tccctcctc aatccattg gttgcatac gaatcaagg 
caaaagacg atggcaaaa agtttaaga cacattgta 
ccgatttaa acgttaaat tagtactaa ccgcgactg 
ctacaccac gggctaacg gccaggtgg agcgattga 
atcgtacga ttttatctc agcttgctg ctagctcga 
gagaagaag agaaatggg atgactttg tgagtagaa 
ttagttggg gtattaatt ctacgccga attcgacta 
cgggcaaaa gtccatacg aattgttgt tggggtata 
cacctcgac atgctaatg actcaatcc tcaccaacg 
tggtgacag agggtgttc atgatggtg atttgccac 
ggacacgag cagacgttg ctcagcgtg tcgaaaagg 
aacagcaga agcaaaaag agcgttatg acaagcgac 
gctgtgccc caaagagat ttaatgcag gtgatctcg 
tgttggtac gcaccacgc gagcttcaa acgagggca 
aaagtcgga agctgttac ctaaatatt cgggaccgt 
accgaattc agaaagtgc tcgactacg ataggtacg 
tcgtgacgg acatgccgg gggcgacgc gttcccaaa 
aacgatatg agggtgttc actcagtgg acaaattga 
gacattacg ttgtcgcca cgacgagtg gagacgaaa 
caataggcg atgagttac gcaaccaca cgctgtcaa 
ttttcattt gggtgtggc tcaaaaggc gcgaaaagt 
tttctggtg agattttcg caatggggc gtcacaggt 
accgaggaa atgtggact cggacgact cggactcaa 
gtgacgatg agccacggc cgaaaaggt ccgcgcctg 
gggtctcca ctacagtaa attcacccc cagtttcgg 
ccgagtccg ctagaatcg cgagacttg agcaacttg 
tggaaaact tgaatcgac gtttgctaa accgcaatt 
ttgagcaca catcaggac aagttggca ttaaaagtg 
gcctgattc ctgaattcg ctccaggaa accccaatt 
ttagtacca ctaagtggt tgcataaaa ttgaccagt 
tggctctcg tgaacggtt gggacgaac gtacaacga 
tttacggca tgataagtc gactcaagg ggttggcca 
aggagtggt acgacaatc tcgatccct cagcatacg 
accggagct gggatgaat ggaaacgtt tgttacaag 
ccaccttcc ccgatcacc acgattatg cttccacac 
ttcgtaagc tagtgaata gggtgaagg aagatgggg 
aaacctggg cacaatatt attttggga aattaaatc 
tgttgcagg cttgtgaca taacaggaa cgaatgcag 
tctcctgtc ttattgatg gaatcactg acgtgactc 
tcagaaatg gggctagag cagggcgtt acgttacac 
ccgaaagct tgtacgctg agtatttgt cgactctga 
gatccgaag gtgacaagc gggatacgc tcgcaaagc 
aggcgcctc gggaaagga ggaggttcc ttccaagtc 
gagtcgaag cacaaaaac tgtattcgt cagttcggt 
gttacaatt gtcgtaatc gaggacatg ttgcgacaa 
agtgcccga aaccaagaa ttgagtgca agaaatgtg 
gtcgcattg gacatgtgg atgctgagt gccgacgtg 
gtaacgtag taaaagaaa acatgtcta agcaggcac 
taacgacaa gtgtagctg acgcaagta ataataaga 
attattaca tagacatta aggttaatg gtaagccga 
cgcgagctt acattgact caggagctg aaccggtcc 
ttgtcaagc aaagtgttg ctagagaat tgggactga 
tgtggcagc caagtttac atttgattc gtggttatg 
gccagggaa tcaccaaag tgcatgggg aagtcgaag 
tagagctag aagtggatc ttgtcaagg caaacgtaa 
aagctttga tcgtcgaag acagtgcac aacgtgtgc 
ctgtcatag tgggacaga ccttcctga atgcgggtg 
ctaatacga ttatagcaa atgaagaag ctgtgagag 
tagttgacg gaaacaacg aatcgatcc aagcatttc 
taggccaac ttccgacac gaaaagttg cactttggg 
cggaagacg aaacagtga tccctcctc aatccattg 
gttgcatac gaatcaagg caaaagacg atgggtgga 
aaggagctt attacgttg aagggtgtc aacgtccga 
gaccagggt tcgaacacg ttgttcatc ggtgtgtca 
catgtgatg gaggcctag tgaccgttc gcaatctat 
cgcaccaag atctggtct atcgaaagg cgcaaatcg 
ttgcgagag ctgagcctt gcgagcagg aatggacag 
ctacgacgc ttgctgcta gctcgagag aagaagaga 
aatgggatg actttgtga gtagaatta gttggggta 
ttaattcta cgccgaatt cgactacgg gcaaaagtc 
catacgaat tgttgttgg ggtatacac ctcgacatg 
ctaatgact caatcctca ccaacgtgg tgacagagg 
gtgttcatg atggtgatt tgccacgga cacgagcag 
acgttgctc agcgtgtcg aaaaggaac agcagaagc 
aaaaagagc gttatgaca agcgacgct gtgccccaa 
agagattta atgcaggtg atctcgtgt tggtacgca 
ccacgcgag cttcaaacg agggcaaaa gtcggaagc 
tgttaccta aatattcgg gaccgtacc gaattcaga 
aagtgctcg actacgata ggtacgtcg tgacggaca 
tgccggggg cgacgcgtt cccaaaaac gatatgagg 
gtgttcact cagtggaca aattgagac attacgttg 
tcgccacga cgagtggag acgaaacaa taggcgatg 
tcagtgacg aatgactga gttcgctgt gaagctgag 
tgaagttca acgtgaact cacgatttt gggcccaaa 
tgttgtttt taagcgctt tacgttgaa actcat
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