| General information | |||
| TC032825
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5773295..5791886 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032825 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06236 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality - Number of animals showing the phenotype: 8 (22 dpi)
Days post injection
- >TC032825 RA
MKVRTKSKG RCWGRTGER FPARTRLCR IWTELSERL RLRRGRGDT SEDDEGLPH SPCNSPTTT EALLDKTAI KDLPTKSHS TCSDGSLLS MGSSEMDEE SFSQQSRHS SKLSLQEKR SSDQDSDLE LGASPSAPL NHSAAHHRV AVRPKRRAP HRNKRVPQL SNALPTTPE VNEEISIRS MTPESVSRE TISEHYSMS TRTTLTETQ LKCSSLPPG LAPPDADSS KLNRSRSNA GSKSEDHFA TLEEDVERE EKTDKSFFD RIFPRRSAK KRKSKEEKT VTVQSSYSK RVESKPIAA PRSGAAARQ RIQPIDLPP SPEVQKREP DSGPPEKSV AGTSPLQAE LESRFKQRQ TLNASLTPP QSPNIDSSS SVITQVEMK TRNEETKTK IKMPGLSSL QQRVLSLND EVDDAGFKS LTDLPDKPA KPVTKSHSF KAAKPEKTE KFVVTEATN ERKISVTKA ASLDSVKHL EEPQYTSEV TQEFTVVKN ESRIKESDS TFSDFNSDV TVSGPSHTA VVNVNYNLA NTITSAEKI KEVTTKECV SVTKIHLKR ESTQVTQST VTVPKNAVP EFLNRQLNK VEAKPSNVV FSMNSPRII DEQTRPKSV EPDKVAKNI PRKFSQEDI EIIEKSEEA PKVATPRFR KNESNKRKS STSSTTSSE SPPVGEKPI LRSRSISLD SLKGETESL DKSSQDSLD KEKTGSVVE TVVLRKKSL IKQRNDEEP ELMKVFARR SLKLKDSES EAVSQQVSV MLDDSKTRD SDKENQAES PQEERKKSV NESNEAPEI REPLTETIK TPTKEQPKI KEKCIELTE VTLRKPGNN FYTRAFSMN AKSGETDEM KVKKQASFS ERRITENWI TNIKNGDDL IERKISQDE IITGISPKS DFITEEVLT ERKNFNQRK AEWEKRAQE AQKKSVP
- >TC032825 RB
MSSAAVGTH HHHSGAGPL SLMSCVRES SPLQDFEPP DTEKNAATK KKFWNPKKW FKRKNKPAE DASVEVIVD KDVLRSRST SELSITEEQ TRRRSGSSM HPGLSVSHD SVFHSPNSG SDIELDAAQ SSSSLSISQ PQIDLRLQT ELSERLRLR RGRGDTSED DEGLPHSPC NSPTTTEAL LDKTAIKDL PTKSHSTCS DGSLLSMGS SEMDEESFS QQSRHSSKL SLQEKRSSD QDSDLELGA SPSAPLNHS AAHHRVAVR PKRRAPHRN KRVPQLSNA LPTTPEVNE EISIRSMTP ESVSRETIS EHYSMSTRT TLTETQLKC SSLPPGLAP PDADSSKLN RSRSNAGSK SEDHFATLE EDVEREEKT DKSFFDRIF PRRSAKKRK SKEEKTVTV QSSYSKRVE SKPIAAPRS GAAARQRIQ PIDLPPSPE VQKREPDSG PPEKSVAGT SPLQAELES RFKQRQTLN ASLTPPQSP NIDSSSSVI TQVEMKTRN EETKTKIKM PGLSSLQQR VLSLNDEVD DAGFKSLTD LPDKPAKPV TKSHSFKAA KPEKTEKFV VTEATNERK ISVTKAASL DSVKHLEEP QYTSEVTQE FTVVKNESR IKESDSTFS DFNSDVTVS GPSHTAVVN VNYNLANTI TSAEKIKEV TTKECVSVT KIHLKREST QVTQSTVTV PKNAVPEFL NRQLNKVEA KPSNVVFSM NSPRIIDEQ TRPKSVEPD KVAKNIPRK FSQEDIEII EKSEEAPKV ATPRFRKNE SNKRKSSTS STTSSESPP VGEKPILRS RSISLDSLK GETESLDKS SQDSLDKEK TGSVVETVV LRKKSLIKQ RNDEEPELM KVFARRSLK LKDSESEAV SQQVSVMLD DSKTRDSDK ENQAESPQE ERKKSVNES NEAPEIREP LTETIKTPT KEQPKIKEK CIELTEVTL RKPGNNFYT RAFSMNAKS GETDEMKVK KQASFSERR ITENWITNI KNGDDLIER KISQDEIIT GISPKSDFI TEEVLTERK NFNQRKAEW EKRAQEAQK KSVP
- >TC032825 RC
MSSAAVGTH HHHSGAGPL SLMSCVRES SPLQDFEPP DTEKNAATK KKFWNPKKW FKRKNKPAE DASVEVIVD KDVLRSRST SELSITEEQ TRRRSGSSM HPGLSVSHD SVFHSPNSG SDIELDAAQ SSSSLSISQ PQIDLRLQV SL
- >TC032825 RA
atgaaagtg cggactaaa tcgaaaggt cgctgttgg ggcaggacc ggtgaacgc tttcctgct aggactagg ctttgcagg atctggaca gagctaagt gagaggctg agattgagg cggggtcgt ggcgacaca agcgaagac gatgaaggc cttccgcat agtccatgc aattctccg accaccacc gaggctctt ctcgacaag actgcaatc aaagatctc cccacgaaa tcacacagt acgtgcagt gatggatcg ttacttagc atgggtagt tcggaaatg gacgaggag agtttcagc caacagtcg cgacactcc tctaaattg tcactacaa gagaagcga tcgtctgat caagattcg gatttggag ttaggagcg tctccctcg gccccctta aaccacagc gcggctcac cacagagtg gccgtgagg cccaaacgg cgggccccc catagaaat aaaagggtg cctcagctg tcaaatgct cttccaacc actccggaa gttaatgaa gaaatatcg ataaggagt atgacgccg gaaagtgtt tccagagaa acgatctct gagcattat agcatgagc acgagaacc actctaacc gaaacgcaa ctaaaatgt tcttctctg cctcctggt ctagcacct cccgatgcc gactccagt aagttgaat cgtagtcgt tcgaacgcc ggcagcaag tcggaggac catttcgcg acgcttgaa gaagacgtc gaacgggag gaaaagacg gacaagtcg ttcttcgac cggatattc cctcgaaga agcgcgaaa aaacgcaaa tcgaaagag gaaaagact gtcaccgtc cagtcgtcg tactcgaag cgggtggaa tcgaagccg atcgcggcc ccaaggtcg ggggcggcg gcacgccag aggatccaa ccgatcgac ctgccgccg tctccggag gtccaaaag cgcgaaccg gattccggc ccaccggaa aaatccgtg gcgggaacg tcgcctctg caagccgag ctggaaagt cggttcaag caaaggcaa actctgaat gctagtctg acgccgcca caatcccca aatatcgat tcgtcgtcg tctgtgata actcaagtc gaaatgaaa acgagaaac gaagaaact aaaactaag ataaaaatg ccgggcttg tcgtcgttg cagcagcgc gttttgtca ctgaacgac gaagttgac gatgccggt tttaagtca ttaactgac ttaccagac aaacctgca aaaccagtc acgaaatca cacagtttc aaagccgca aagccggaa aaaaccgaa aaattcgtc gttactgaa gcaactaac gagaggaag atttcggta acgaaagcc gcgtcgctc gacagtgtc aaacacttg gaggagcct caatacacc tccgaggtg acacaggag tttacagtt gttaagaac gaatctaga atcaaagaa tcggactca actttctct gattttaat tcggacgtt accgtttcg ggccccagt cacactgcg gtcgttaac gttaattac aacctggcc aacacgata acttccgcc gagaaaatt aaagaagtt accacgaaa gaatgcgtt tccgtaaca aaaatccac ttgaaacgc gagtctacc caagtaaca caaagcaca gttacagtt cctaaaaac gcggtcccc gaatttcta aatcggcag ttgaacaaa gtcgaggcg aagccgagc aatgtggtt ttttccatg aattctccg aggattatc gacgagcaa actcggccc aaatcagtc gaaccggac aaggttgct aagaacatc ccgcggaag tttagccaa gaagatatc gaaataatc gagaagagt gaggaagcg cccaaggtg gcgactcca aggtttcgg aaaaacgag tcaaacaag aggaaatcg tcgacgagt tcgaccaca agttccgag tcgccgccg gtcggcgaa aaaccgatc ttgcggagt cgctcgatc agtttggac tcgctgaag ggcgaaacc gagagtttg gacaagtcg tctcaggac agtttggac aaggagaaa acggggtcg gtggttgag acggtagtt ttgcgcaaa aaatcgcta atcaagcag aggaacgac gaagagccc gagttgatg aaagtgttt gcgcgccgt tcgctcaaa ctcaaagac tcagagagc gaggcggtg agtcagcaa gtgagcgtg atgctcgac gacagcaaa accagagac agtgataag gaaaatcaa gcggaatcg ccgcaagaa gagcgcaaa aaatccgta aatgaatca aacgaagcg cccgaaatc cgggaaccg ttaaccgag acgatcaaa acgccgacc aaggagcag ccgaaaatc aaagagaag tgcatcgag ttgaccgaa gtgacgttg cgcaagccg gggaataat ttttacacg agggcgttc agtatgaat gcgaaatcg ggcgagacg gacgaaatg aaagtgaag aagcaggcg agtttcagc gagaggaga atcacggag aattggata acgaatatt aagaacggt gatgattta attgagagg aaaatttcg caggacgaa attattacc gggatatcg ccgaaaagt gacttcata accgaggag gtattgacc gagcggaaa aacttcaat cagcggaaa gccgagtgg gagaaaagg gcacaagag gcacagaaa aagtcggta ccgtag
- >TC032825 RB
atgtcatcg gcggctgtg gggacacat caccatcat tcgggggct ggtccccta agtctgatg tcatgcgtc cgtgagtcg tcgccgttg caggatttc gagcctccc gacaccgaa aagaacgcc gcgacgaaa aagaagttt tggaacccg aagaagtgg ttcaagagg aagaacaag ccggccgag gatgcttct gtcgaagtc atcgtcgac aaggatgta ctccggagc aggtccacc agcgagctt tccatcaca gaggagcaa acgcggaga agaagtggg tcctcaatg catcccgga ttgagtgtt tcccacgac agtgtcttc cattcgccg aattccggc tcagacatc gagttagat gcggcacaa agctcgtcc tctttatcc atatcgcaa cctcaaatt gatctgagg ttacagaca gagctaagt gagaggctg agattgagg cggggtcgt ggcgacaca agcgaagac gatgaaggc cttccgcat agtccatgc aattctccg accaccacc gaggctctt ctcgacaag actgcaatc aaagatctc cccacgaaa tcacacagt acgtgcagt gatggatcg ttacttagc atgggtagt tcggaaatg gacgaggag agtttcagc caacagtcg cgacactcc tctaaattg tcactacaa gagaagcga tcgtctgat caagattcg gatttggag ttaggagcg tctccctcg gccccctta aaccacagc gcggctcac cacagagtg gccgtgagg cccaaacgg cgggccccc catagaaat aaaagggtg cctcagctg tcaaatgct cttccaacc actccggaa gttaatgaa gaaatatcg ataaggagt atgacgccg gaaagtgtt tccagagaa acgatctct gagcattat agcatgagc acgagaacc actctaacc gaaacgcaa ctaaaatgt tcttctctg cctcctggt ctagcacct cccgatgcc gactccagt aagttgaat cgtagtcgt tcgaacgcc ggcagcaag tcggaggac catttcgcg acgcttgaa gaagacgtc gaacgggag gaaaagacg gacaagtcg ttcttcgac cggatattc cctcgaaga agcgcgaaa aaacgcaaa tcgaaagag gaaaagact gtcaccgtc cagtcgtcg tactcgaag cgggtggaa tcgaagccg atcgcggcc ccaaggtcg ggggcggcg gcacgccag aggatccaa ccgatcgac ctgccgccg tctccggag gtccaaaag cgcgaaccg gattccggc ccaccggaa aaatccgtg gcgggaacg tcgcctctg caagccgag ctggaaagt cggttcaag caaaggcaa actctgaat gctagtctg acgccgcca caatcccca aatatcgat tcgtcgtcg tctgtgata actcaagtc gaaatgaaa acgagaaac gaagaaact aaaactaag ataaaaatg ccgggcttg tcgtcgttg cagcagcgc gttttgtca ctgaacgac gaagttgac gatgccggt tttaagtca ttaactgac ttaccagac aaacctgca aaaccagtc acgaaatca cacagtttc aaagccgca aagccggaa aaaaccgaa aaattcgtc gttactgaa gcaactaac gagaggaag atttcggta acgaaagcc gcgtcgctc gacagtgtc aaacacttg gaggagcct caatacacc tccgaggtg acacaggag tttacagtt gttaagaac gaatctaga atcaaagaa tcggactca actttctct gattttaat tcggacgtt accgtttcg ggccccagt cacactgcg gtcgttaac gttaattac aacctggcc aacacgata acttccgcc gagaaaatt aaagaagtt accacgaaa gaatgcgtt tccgtaaca aaaatccac ttgaaacgc gagtctacc caagtaaca caaagcaca gttacagtt cctaaaaac gcggtcccc gaatttcta aatcggcag ttgaacaaa gtcgaggcg aagccgagc aatgtggtt ttttccatg aattctccg aggattatc gacgagcaa actcggccc aaatcagtc gaaccggac aaggttgct aagaacatc ccgcggaag tttagccaa gaagatatc gaaataatc gagaagagt gaggaagcg cccaaggtg gcgactcca aggtttcgg aaaaacgag tcaaacaag aggaaatcg tcgacgagt tcgaccaca agttccgag tcgccgccg gtcggcgaa aaaccgatc ttgcggagt cgctcgatc agtttggac tcgctgaag ggcgaaacc gagagtttg gacaagtcg tctcaggac agtttggac aaggagaaa acggggtcg gtggttgag acggtagtt ttgcgcaaa aaatcgcta atcaagcag aggaacgac gaagagccc gagttgatg aaagtgttt gcgcgccgt tcgctcaaa ctcaaagac tcagagagc gaggcggtg agtcagcaa gtgagcgtg atgctcgac gacagcaaa accagagac agtgataag gaaaatcaa gcggaatcg ccgcaagaa gagcgcaaa aaatccgta aatgaatca aacgaagcg cccgaaatc cgggaaccg ttaaccgag acgatcaaa acgccgacc aaggagcag ccgaaaatc aaagagaag tgcatcgag ttgaccgaa gtgacgttg cgcaagccg gggaataat ttttacacg agggcgttc agtatgaat gcgaaatcg ggcgagacg gacgaaatg aaagtgaag aagcaggcg agtttcagc gagaggaga atcacggag aattggata acgaatatt aagaacggt gatgattta attgagagg aaaatttcg caggacgaa attattacc gggatatcg ccgaaaagt gacttcata accgaggag gtattgacc gagcggaaa aacttcaat cagcggaaa gccgagtgg gagaaaagg gcacaagag gcacagaaa aagtcggta ccgtag
- >TC032825 RC
atgtcatcg gcggctgtg gggacacat caccatcat tcgggggct ggtccccta agtctgatg tcatgcgtc cgtgagtcg tcgccgttg caggatttc gagcctccc gacaccgaa aagaacgcc gcgacgaaa aagaagttt tggaacccg aagaagtgg ttcaagagg aagaacaag ccggccgag gatgcttct gtcgaagtc atcgtcgac aaggatgta ctccggagc aggtccacc agcgagctt tccatcaca gaggagcaa acgcggaga agaagtggg tcctcaatg catcccgga ttgagtgtt tcccacgac agtgtcttc cattcgccg aattccggc tcagacatc gagttagat gcggcacaa agctcgtcc tctttatcc atatcgcaa cctcaaatt gatctgagg ttacaggtg agtctttga
- >TC032825 RA
tgaggtcgg tgcaaagtt gccagacgg gtttgaaac accgcagtt tgcaggtcc tcgtgtcca gaaaaagct cgaataagt aaacacagc cccaaaatg tggattaaa gcgcagtaa tttagtgac agagtgagt aatatcgta agaaaaacc gcgattcgg ctgaagccg tgacagaat ttcctgcga ttagataaa tattcctgc ttgaaattc agtgcctgt gtctcgatc taatttatt acatgaaat gcagtggtt tgtgtcaat gaaatgaaa gtgcggact aaatcgaaa ggtcgctgt tggggcagg accggtgaa cgctttcct gctaggact aggctttgc aggatctgg acagagcta agtgagagg ctgagattg aggcggggt cgtggcgac acaagcgaa gacgatgaa ggccttccg catagtcca tgcaattct ccgaccacc accgaggct cttctcgac aagactgca atcaaagat ctccccacg aaatcacac agtacgtgc agtgatgga tcgttactt agcatgggt agttcggaa atggacgag gagagtttc agccaacag tcgcgacac tcctctaaa ttgtcacta caagagaag cgatcgtct gatcaagat tcggatttg gagttagga gcgtctccc tcggccccc ttaaaccac agcgcggct caccacaga gtggccgtg aggcccaaa cggcgggcc ccccataga aataaaagg gtgcctcag ctgtcaaat gctcttcca accactccg gaagttaat gaagaaata tcgataagg agtatgacg ccggaaagt gtttccaga gaaacgatc tctgagcat tatagcatg agcacgaga accactcta accgaaacg caactaaaa tgttcttct ctgcctcct ggtctagca cctcccgat gccgactcc agtaagttg aatcgtagt cgttcgaac gccggcagc aagtcggag gaccatttc gcgacgctt gaagaagac gtcgaacgg gaggaaaag acggacaag tcgttcttc gaccggata ttccctcga agaagcgcg aaaaaacgc aaatcgaaa gaggaaaag actgtcacc gtccagtcg tcgtactcg aagcgggtg gaatcgaag ccgatcgcg gccccaagg tcgggggcg gcggcacgc cagaggatc caaccgatc gacctgccg ccgtctccg gaggtccaa aagcgcgaa ccggattcc ggcccaccg gaaaaatcc gtggcggga acgtcgcct ctgcaagcc gagctggaa agtcggttc aagcaaagg caaactctg aatgctagt ctgacgccg ccacaatcc ccaaatatc gattcgtcg tcgtctgtg ataactcaa gtcgaaatg aaaacgaga aacgaagaa actaaaact aagataaaa atgccgggc ttgtcgtcg ttgcagcag cgcgttttg tcactgaac gacgaagtt gacgatgcc ggttttaag tcattaact gacttacca gacaaacct gcaaaacca gtcacgaaa tcacacagt ttcaaagcc gcaaagccg gaaaaaacc gaaaaattc gtcgttact gaagcaact aacgagagg aagatttcg gtaacgaaa gccgcgtcg ctcgacagt gtcaaacac ttggaggag cctcaatac acctccgag gtgacacag gagtttaca gttgttaag aacgaatct agaatcaaa gaatcggac tcaactttc tctgatttt aattcggac gttaccgtt tcgggcccc agtcacact gcggtcgtt aacgttaat tacaacctg gccaacacg ataacttcc gccgagaaa attaaagaa gttaccacg aaagaatgc gtttccgta acaaaaatc cacttgaaa cgcgagtct acccaagta acacaaagc acagttaca gttcctaaa aacgcggtc cccgaattt ctaaatcgg cagttgaac aaagtcgag gcgaagccg agcaatgtg gttttttcc atgaattct ccgaggatt atcgacgag caaactcgg cccaaatca gtcgaaccg gacaaggtt gctaagaac atcccgcgg aagtttagc caagaagat atcgaaata atcgagaag agtgaggaa gcgcccaag gtggcgact ccaaggttt cggaaaaac gagtcaaac aagaggaaa tcgtcgacg agttcgacc acaagttcc gagtcgccg ccggtcggc gaaaaaccg atcttgcgg agtcgctcg atcagtttg gactcgctg aagggcgaa accgagagt ttggacaag tcgtctcag gacagtttg gacaaggag aaaacgggg tcggtggtt gagacggta gttttgcgc aaaaaatcg ctaatcaag cagaggaac gacgaagag cccgagttg atgaaagtg tttgcgcgc cgttcgctc aaactcaaa gactcagag agcgaggcg gtgagtcag caagtgagc gtgatgctc gacgacagc aaaaccaga gacagtgat aaggaaaat caagcggaa tcgccgcaa gaagagcgc aaaaaatcc gtaaatgaa tcaaacgaa gcgcccgaa atccgggaa ccgttaacc gagacgatc aaaacgccg accaaggag cagccgaaa atcaaagag aagtgcatc gagttgacc gaagtgacg ttgcgcaag ccggggaat aatttttac acgagggcg ttcagtatg aatgcgaaa tcgggcgag acggacgaa atgaaagtg aagaagcag gcgagtttc agcgagagg agaatcacg gagaattgg ataacgaat attaagaac ggtgatgat ttaattgag aggaaaatt tcgcaggac gaaattatt accgggata tcgccgaaa agtgacttc ataaccgag gaggtattg accgagcgg aaaaacttc aatcagcgg aaagccgag tgggagaaa agggcacaa gaggcacag aaaaagtcg gtaccgtag ttatgtaag tggcattga ttttttgga aattgtcgc gcgaagtga tgataaata gggtgagat ttgtttcaa ttttcaggt ggtgcaacc aatccaatg acccggctt ggagctagt tgtgaaaat ttagtcagc gttatgtca gtgtaaata atgttcgtg ttacttctg tgtataaca tcacatttt tgtatcgtt ttttttttt aagtgagtt ggtgccatg tgatgatac cggcattaa tgtctaaaa aaagtaatt tgtattatt gtatatttt atactgtag ataagtgcc tttttaaac ttttttaat atgttgatt cagaatgtg atgaagttg tttcgattg ttgaccaat ggaaaaagc cttgcgagt tgtggtgct tgagagata attaagaaa tacagagtt atgtatgta tacttcaaa taaatgtta ataatgcag tatgggaat atgtctaat tactgatat taaatgttg aactgcaat aaagtattt atagaaac
- >TC032825 RB
taatgaact aatttttgt ttttatttt caggtttca cgatgtacg ccgataagt gtgaaaaat cgtagcgtg gctctgctg aaatgtcat cggcggctg tggggacac atcaccatc attcggggg ctggtcccc taagtctga tgtcatgcg tccgtgagt cgtcgccgt tgcaggatt tcgagcctc ccgacaccg aaaagaacg ccgcgacga aaaagaagt tttggaacc cgaagaagt ggttcaaga ggaagaaca agccggccg aggatgctt ctgtcgaag tcatcgtcg acaaggatg tactccgga gcaggtcca ccagcgagc tttccatca cagaggagc aaacgcgga gaagaagtg ggtcctcaa tgcatcccg gattgagtg tttcccacg acagtgtct tccattcgc cgaattccg gctcagaca tcgagttag atgcggcac aaagctcgt cctctttat ccatatcgc aacctcaaa ttgatctga ggttacaga cagagctaa gtgagaggc tgagattga ggcggggtc gtggcgaca caagcgaag acgatgaag gccttccgc atagtccat gcaattctc cgaccacca ccgaggctc ttctcgaca agactgcaa tcaaagatc tccccacga aatcacaca gtacgtgca gtgatggat cgttactta gcatgggta gttcggaaa tggacgagg agagtttca gccaacagt cgcgacact cctctaaat tgtcactac aagagaagc gatcgtctg atcaagatt cggatttgg agttaggag cgtctccct cggccccct taaaccaca gcgcggctc accacagag tggccgtga ggcccaaac ggcgggccc cccatagaa ataaaaggg tgcctcagc tgtcaaatg ctcttccaa ccactccgg aagttaatg aagaaatat cgataagga gtatgacgc cggaaagtg tttccagag aaacgatct ctgagcatt atagcatga gcacgagaa ccactctaa ccgaaacgc aactaaaat gttcttctc tgcctcctg gtctagcac ctcccgatg ccgactcca gtaagttga atcgtagtc gttcgaacg ccggcagca agtcggagg accatttcg cgacgcttg aagaagacg tcgaacggg aggaaaaga cggacaagt cgttcttcg accggatat tccctcgaa gaagcgcga aaaaacgca aatcgaaag aggaaaaga ctgtcaccg tccagtcgt cgtactcga agcgggtgg aatcgaagc cgatcgcgg ccccaaggt cgggggcgg cggcacgcc agaggatcc aaccgatcg acctgccgc cgtctccgg aggtccaaa agcgcgaac cggattccg gcccaccgg aaaaatccg tggcgggaa cgtcgcctc tgcaagccg agctggaaa gtcggttca agcaaaggc aaactctga atgctagtc tgacgccgc cacaatccc caaatatcg attcgtcgt cgtctgtga taactcaag tcgaaatga aaacgagaa acgaagaaa ctaaaacta agataaaaa tgccgggct tgtcgtcgt tgcagcagc gcgttttgt cactgaacg acgaagttg acgatgccg gttttaagt cattaactg acttaccag acaaacctg caaaaccag tcacgaaat cacacagtt tcaaagccg caaagccgg aaaaaaccg aaaaattcg tcgttactg aagcaacta acgagagga agatttcgg taacgaaag ccgcgtcgc tcgacagtg tcaaacact tggaggagc ctcaataca cctccgagg tgacacagg agtttacag ttgttaaga acgaatcta gaatcaaag aatcggact caactttct ctgatttta attcggacg ttaccgttt cgggcccca gtcacactg cggtcgtta acgttaatt acaacctgg ccaacacga taacttccg ccgagaaaa ttaaagaag ttaccacga aagaatgcg tttccgtaa caaaaatcc acttgaaac gcgagtcta cccaagtaa cacaaagca cagttacag ttcctaaaa acgcggtcc ccgaatttc taaatcggc agttgaaca aagtcgagg cgaagccga gcaatgtgg ttttttcca tgaattctc cgaggatta tcgacgagc aaactcggc ccaaatcag tcgaaccgg acaaggttg ctaagaaca tcccgcgga agtttagcc aagaagata tcgaaataa tcgagaaga gtgaggaag cgcccaagg tggcgactc caaggtttc ggaaaaacg agtcaaaca agaggaaat cgtcgacga gttcgacca caagttccg agtcgccgc cggtcggcg aaaaaccga tcttgcgga gtcgctcga tcagtttgg actcgctga agggcgaaa ccgagagtt tggacaagt cgtctcagg acagtttgg acaaggaga aaacggggt cggtggttg agacggtag ttttgcgca aaaaatcgc taatcaagc agaggaacg acgaagagc ccgagttga tgaaagtgt ttgcgcgcc gttcgctca aactcaaag actcagaga gcgaggcgg tgagtcagc aagtgagcg tgatgctcg acgacagca aaaccagag acagtgata aggaaaatc aagcggaat cgccgcaag aagagcgca aaaaatccg taaatgaat caaacgaag cgcccgaaa tccgggaac cgttaaccg agacgatca aaacgccga ccaaggagc agccgaaaa tcaaagaga agtgcatcg agttgaccg aagtgacgt tgcgcaagc cggggaata atttttaca cgagggcgt tcagtatga atgcgaaat cgggcgaga cggacgaaa tgaaagtga agaagcagg cgagtttca gcgagagga gaatcacgg agaattgga taacgaata ttaagaacg gtgatgatt taattgaga ggaaaattt cgcaggacg aaattatta ccgggatat cgccgaaaa gtgacttca taaccgagg aggtattga ccgagcgga aaaacttca atcagcgga aagccgagt gggagaaaa gggcacaag aggcacaga aaaagtcgg taccgtagt tatgtaagt ggcattgat tttttggaa attgtcgcg cgaagtgat gataaatag ggtgagatt tgtttcaat tttcaggtg gtgcaacca atccaatga cccggcttg gagctagtt gtgaaaatt tagtcagcg ttatgtcag tgtaaataa tgttcgtgt tacttctgt gtataacat cacattttt gtatcgttt tttttttta agtgagttg gtgccatgt gatgatacc ggcattaat gtctaaaaa aagtaattt gtattattg tatatttta tactgtaga taagtgcct ttttaaact tttttaata tgttgattc agaatgtga tgaagttgt ttcgattgt tgaccaatg gaaaaagcc ttgcgagtt gtggtgctt gagagataa ttaagaaat acagagtta tgtatgtat acttcaaat aaatgttaa taatgcagt atgggaata tgtctaatt actgatatt aaatgttga actgcaata aagtattta tagaaac
- >TC032825 RC
taatgaact aatttttgt ttttatttt caggtttca cgatgtacg ccgataagt gtgaaaaat cgtagcgtg gctctgctg aaatgtcat cggcggctg tggggacac atcaccatc attcggggg ctggtcccc taagtctga tgtcatgcg tccgtgagt cgtcgccgt tgcaggatt tcgagcctc ccgacaccg aaaagaacg ccgcgacga aaaagaagt tttggaacc cgaagaagt ggttcaaga ggaagaaca agccggccg aggatgctt ctgtcgaag tcatcgtcg acaaggatg tactccgga gcaggtcca ccagcgagc tttccatca cagaggagc aaacgcgga gaagaagtg ggtcctcaa tgcatcccg gattgagtg tttcccacg acagtgtct tccattcgc cgaattccg gctcagaca tcgagttag atgcggcac aaagctcgt cctctttat ccatatcgc aacctcaaa ttgatctga ggttacagg tgagtcttt gacgattct gtttgcata caaacgcgg aaaattcgt ccttggtgc cgaaaataa atccggttt gttttc
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