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Gene information
General information
TC007307 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6285477..6288808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007307
      iBeetle screen iB_06234

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      remarks: eggs lost,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007307 RA
        MLINQQICC VLLFLVTVN SETSDSSER GKRTIGSWL 
        TNIGDLFGY DVHKRPSMV RPPVTVLTA AVPANLIAG 
        PVFARQKAV GPAPPPKSA PAPAPAPAP APAPAPAPA 
        PAPAPAPAP APAPQLLTE SVQKTFNVA FNWGKNVQT 
        LPPPGKAAP VLIPVAVPA PAPAPAPAP APAPAPAPA 
        PAPAPAPAP PAKPGAPPK PPSKVQLLR EYDEDYPAR 
        LDLPEKVQI ELPDYSTDS GSEKREINY DYLHSKENF 
        KEGFKTFWD NSPYTAEHG YKFLAPQAN SQENFQKDI 
        PLSKYEEYD LKPKHHNSI RSVSTPKFF VNSHSLNID 
        ETPLLEVHD VNGDLAKQN IELHRHILE YYKKKFAAD 
        AETARRQAH QLAPPQKIT YQAVGPEKQ YLTLERLDK 
        IIQRGRDNY YKKENLRNY GEEVEETPA PVLHSKNPE 
        RENLKHYEE EVEEIPAPV FHSKNPERE NLKNYEEEV 
        EEIPAPVFH LKNPERENL KNHKEENEE RSLKSASPK 
        DDVEEISKT KKDEPKKEE EEEEIEEVP PTEFTKSLK 
        FDPEKKFQE FKFLPPEDT SKEVKGVDR TIDDEISSN 
        FQRNHKKYE DIKDDFLQK HEDKEEISE KLRKQPTPA 
        PFKIVDVDE KENFDYEML PHLSEESKS EDIKEEKNL 
        EKEEPHVED HDKQFENIR QEFLDREKQ YTGYTPEEK 
        RRDSNEKEI IAFHDVDGN VYRENAEEG EEYEGQNED 
        YDEGKKEVE QKAVNDHYA NYNRDDGNK FRIYFDYEN 
        EEEKLPFYT GDIEGYVKH EFENDRNNL KQKTEQNLE 
        NYYRSLEEG DPNQGEESE KVQQDCETD EEKEPENEE 
        TKQEEEKQS LKKVKGFFV APTQAQNLA PNLYEGIDH 
        ASAFLSIPN YFGEHQQAA PGFEHNFGK NFPVKQQQA 
        ATQNSEVVQ PLYSGIISV GALQELDKS NKPLTIPFD 
        FDYSASEIK VKKTKVAFD SKPAATEYT GYESHYVPE 
        KYTEDFSYT VPEPPPVDY AKLEAKDFE DDDTLSKNA 
        KILNLESGR TEAFLTING NKYHL
      CDS sequence
      • >TC007307 RA
        atgctcata aatcaacaa atctgttgt gttctcctg 
        ttcctagtg actgtaaat agtgaaact agtgactcc 
        agtgaacgt ggaaagaga acgataggc tcatggcta 
        acaaacatc ggagacctt ttcggttat gacgtgcac 
        aaacggccg tcaatggtg cggccccca gtgactgtc 
        ctcacagcc gcagtgccc gcaaaccta atcgcagga 
        ccggtcttc gcccgacag aaggcagtg ggcccggcg 
        cctccgcca aaatcggcc cctgcacca gccccggcc 
        ccggcccca gccccagca cctgcccca gctccggcc 
        ccggccccg gccccagcc cccgcacca gccccagcc 
        ccgcaacta ctaacagaa tcggtgcaa aaaacattc 
        aatgtcgcc ttcaactgg ggaaagaac gtccagact 
        ttaccacct ccggggaaa gcggccccg gttttaatt 
        ccggttgct gtcccggct ccggcaccg gccccagcc 
        ccggctccg gcgcccgct ccggcacct gcccccgct 
        ccggcccca gctccggcg cccgcgcct ccggcgaaa 
        cccggagcg cccccaaaa ccgccctcc aaagtccag 
        ttactgcga gagtacgat gaggattat cccgcaagg 
        ttagattta ccggaaaag gtccaaatc gaactcccg 
        gattactcg actgactcc ggatcggaa aagcgtgaa 
        attaattac gattatctc cattcgaaa gagaatttt 
        aaagaagga tttaaaacg ttttgggat aattctcct 
        tacaccgca gaacatggc tataagttt ctagcaccg 
        caagcgaat tcgcaagaa aatttccaa aaagatatt 
        ccattatcg aaatacgag gagtacgat ttgaaacct 
        aagcaccat aacagtatt cgaagtgtt tcaactccg 
        aaatttttt gttaattcc cacagtttg aacatcgat 
        gagacgcca cttttggaa gttcacgac gttaatggt 
        gatttagcc aaacaaaat attgagctt catagacac 
        attttggaa tattataaa aaaaaattt gcagcggat 
        gcggaaact gcacggcga caagcgcat caacttgcg 
        cccccgcaa aaaatcacc taccaagct gttggcccc 
        gaaaaacaa tacctgact ctagaacgg ctagataaa 
        atcattcag agagggaga gataattac tacaaaaag 
        gaaaacttg agaaattac ggggaagaa gttgaggaa 
        actcccgcc ccggttttg cactcgaaa aatcccgaa 
        agagaaaac ttgaaacat tacgaggaa gaagttgag 
        gaaattccc gctccggtt ttccactcg aaaaatccc 
        gaaagagaa aacttgaaa aattacgag gaagaagtt 
        gaggaaatt cctgctccg gtgttccat ttgaaaaat 
        cctgaaaga gaaaacttg aaaaaccat aaagaagaa 
        aatgaagaa cgatctttg aaaagtgcg tcaccgaag 
        gatgatgtt gaagagatc agcaaaacg aagaaagat 
        gagccaaaa aaagaggag gaagaggaa gaaattgaa 
        gaagtgcca ccgaccgaa ttcaccaag agtctcaaa 
        ttcgatcct gaaaaaaag tttcaggag tttaagttt 
        cttccccct gaagacacg agtaaagag gttaagggc 
        gttgacagg accatcgat gatgaaata tcctcaaat 
        tttcaaaga aatcacaag aaatacgaa gacataaag 
        gacgatttt ttgcaaaaa catgaagat aaggaggag 
        ataagtgag aaattgagg aagcaacca acccctgcg 
        ccttttaaa atcgtggac gttgacgag aaggaaaat 
        tttgactac gaaatgttg ccacacttg agcgaagaa 
        agcaaaagt gaagacata aaggaagag aaaaatttg 
        gagaaggag gagcctcat gttgaagac cacgacaaa 
        cagtttgaa aatattcgg caagaattt ctagatcga 
        gaaaagcag tacacggga tatacgccc gaagaaaaa 
        cgccgggat tccaacgag aaagaaata atcgcattc 
        catgacgtt gatggaaat gtttatcgc gaaaatgcg 
        gaagaaggg gaagaatac gaaggccag aatgaagat 
        tacgacgag ggcaaaaaa gaagtggaa cagaaagcc 
        gtcaacgat cattacgct aattacaac cgagacgat 
        ggaaataaa ttccgaatt tacttcgat tacgaaaac 
        gaagaagaa aaactgcct ttctacacc ggagacatt 
        gagggttac gtaaaacac gaattcgaa aacgaccga 
        aataacttg aagcaaaaa acggaacaa aacctcgaa 
        aactactac cgcagtctg gaggaaggg gaccccaac 
        cagggcgaa gagagtgaa aaggtgcaa caagactgc 
        gaaacagac gaagaaaaa gaaccagaa aatgaagaa 
        acgaaacaa gaagaagaa aaacagtcc ttgaagaaa 
        gtcaaggga tttttcgta gcaccgaca caagcccag 
        aacttggct ccgaattta tacgaaggg atcgaccac 
        gctagtgcc tttctatca attcctaat tacttcggc 
        gagcatcag caggcagcc cccggattt gagcacaat 
        ttcgggaag aatttcccc gtgaagcaa caacaagcc 
        gccacgcaa aattccgaa gtcgttcag ccgctctac 
        tccggtatt attagcgtg ggggcgttg caagagctg 
        gacaagtct aacaaaccg ttgacgatt ccgttcgat 
        tttgattac agcgcgtct gaaatcaag gtgaagaag 
        acgaaagtc gcctttgat agtaaacca gccgcgacg 
        gaatataca gggtatgag agtcattat gtgcctgag 
        aaatataca gaggacttt tcttatact gttccagaa 
        ccgccaccg gttgattac gcgaaactc gaagctaaa 
        gatttcgag gatgatgat acgctttcg aaaaatgct 
        aaaatttta aatttggag tcggggaga acggaggct 
        tttttgacc attaacggg aacaagtat catctctag
      mRNA sequence
      • >TC007307 RA
        aagagcatg gcacaatgc tcataaatc aacaaatct 
        gttgtgttc tcctgttcc tagtgactg taaatagtg 
        aaactagtg actccagtg aacgtggaa agagaacga 
        taggctcat ggctaacaa acatcggag accttttcg 
        gttatgacg tgcacaaac ggccgtcaa tggtgcggc 
        ccccagtga ctgtcctca cagccgcag tgcccgcaa 
        acctaatcg caggaccgg tcttcgccc gacagaagg 
        cagtgggcc cggcgcctc cgccaaaat cggcccctg 
        caccagccc cggccccgg ccccagccc cagcacctg 
        ccccagctc cggccccgg ccccggccc cagcccccg 
        caccagccc cagccccgc aactactaa cagaatcgg 
        tgcaaaaaa cattcaatg tcgccttca actggggaa 
        agaacgtcc agactttac cacctccgg ggaaagcgg 
        ccccggttt taattccgg ttgctgtcc cggctccgg 
        caccggccc cagccccgg ctccggcgc ccgctccgg 
        cacctgccc ccgctccgg ccccagctc cggcgcccg 
        cgcctccgg cgaaacccg gagcgcccc caaaaccgc 
        cctccaaag tccagttac tgcgagagt acgatgagg 
        attatcccg caaggttag atttaccgg aaaaggtcc 
        aaatcgaac tcccggatt actcgactg actccggat 
        cggaaaagc gtgaaatta attacgatt atctccatt 
        cgaaagaga attttaaag aaggattta aaacgtttt 
        gggataatt ctccttaca ccgcagaac atggctata 
        agtttctag caccgcaag cgaattcgc aagaaaatt 
        tccaaaaag atattccat tatcgaaat acgaggagt 
        acgatttga aacctaagc accataaca gtattcgaa 
        gtgtttcaa ctccgaaat tttttgtta attcccaca 
        gtttgaaca tcgatgaga cgccacttt tggaagttc 
        acgacgtta atggtgatt tagccaaac aaaatattg 
        agcttcata gacacattt tggaatatt ataaaaaaa 
        aatttgcag cggatgcgg aaactgcac ggcgacaag 
        cgcatcaac ttgcgcccc cgcaaaaaa tcacctacc 
        aagctgttg gccccgaaa aacaatacc tgactctag 
        aacggctag ataaaatca ttcagagag ggagagata 
        attactaca aaaaggaaa acttgagaa attacgggg 
        aagaagttg aggaaactc ccgccccgg ttttgcact 
        cgaaaaatc ccgaaagag aaaacttga aacattacg 
        aggaagaag ttgaggaaa ttcccgctc cggttttcc 
        actcgaaaa atcccgaaa gagaaaact tgaaaaatt 
        acgaggaag aagttgagg aaattcctg ctccggtgt 
        tccatttga aaaatcctg aaagagaaa acttgaaaa 
        accataaag aagaaaatg aagaacgat ctttgaaaa 
        gtgcgtcac cgaaggatg atgttgaag agatcagca 
        aaacgaaga aagatgagc caaaaaaag aggaggaag 
        aggaagaaa ttgaagaag tgccaccga ccgaattca 
        ccaagagtc tcaaattcg atcctgaaa aaaagtttc 
        aggagttta agtttcttc cccctgaag acacgagta 
        aagaggtta agggcgttg acaggacca tcgatgatg 
        aaatatcct caaattttc aaagaaatc acaagaaat 
        acgaagaca taaaggacg attttttgc aaaaacatg 
        aagataagg aggagataa gtgagaaat tgaggaagc 
        aaccaaccc ctgcgcctt ttaaaatcg tggacgttg 
        acgagaagg aaaattttg actacgaaa tgttgccac 
        acttgagcg aagaaagca aaagtgaag acataaagg 
        aagagaaaa atttggaga aggaggagc ctcatgttg 
        aagaccacg acaaacagt ttgaaaata ttcggcaag 
        aatttctag atcgagaaa agcagtaca cgggatata 
        cgcccgaag aaaaacgcc gggattcca acgagaaag 
        aaataatcg cattccatg acgttgatg gaaatgttt 
        atcgcgaaa atgcggaag aaggggaag aatacgaag 
        gccagaatg aagattacg acgagggca aaaaagaag 
        tggaacaga aagccgtca acgatcatt acgctaatt 
        acaaccgag acgatggaa ataaattcc gaatttact 
        tcgattacg aaaacgaag aagaaaaac tgcctttct 
        acaccggag acattgagg gttacgtaa aacacgaat 
        tcgaaaacg accgaaata acttgaagc aaaaaacgg 
        aacaaaacc tcgaaaact actaccgca gtctggagg 
        aaggggacc ccaaccagg gcgaagaga gtgaaaagg 
        tgcaacaag actgcgaaa cagacgaag aaaaagaac 
        cagaaaatg aagaaacga aacaagaag aagaaaaac 
        agtccttga agaaagtca agggatttt tcgtagcac 
        cgacacaag cccagaact tggctccga atttatacg 
        aagggatcg accacgcta gtgcctttc tatcaattc 
        ctaattact tcggcgagc atcagcagg cagcccccg 
        gatttgagc acaatttcg ggaagaatt tccccgtga 
        agcaacaac aagccgcca cgcaaaatt ccgaagtcg 
        ttcagccgc tctactccg gtattatta gcgtggggg 
        cgttgcaag agctggaca agtctaaca aaccgttga 
        cgattccgt tcgattttg attacagcg cgtctgaaa 
        tcaaggtga agaagacga aagtcgcct ttgatagta 
        aaccagccg cgacggaat atacagggt atgagagtc 
        attatgtgc ctgagaaat atacagagg acttttctt 
        atactgttc cagaaccgc caccggttg attacgcga 
        aactcgaag ctaaagatt tcgaggatg atgatacgc 
        tttcgaaaa atgctaaaa ttttaaatt tggagtcgg 
        ggagaacgg aggcttttt tgaccatta acgggaaca 
        agtatcatc tctaggaat cggtagaaa atcatagtc 
        taatttatt attacacgt gaccaaata gcgttatgt 
        tgaaaataa gtttttata ttgttgcac catttgtta 
        aaaaattat gtaaataac tacaaagat cttacttat 
        ttaatgaca ctcattatc gtgcttttt aagtttata 
        ttattgctt gttaactat ttgtataat gttggtatt 
        aataaagtt taaaacata ag
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY