| General information | |||
| TC007307 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6285477..6288808 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC007307 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06234 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC007307 RA
MLINQQICC VLLFLVTVN SETSDSSER GKRTIGSWL TNIGDLFGY DVHKRPSMV RPPVTVLTA AVPANLIAG PVFARQKAV GPAPPPKSA PAPAPAPAP APAPAPAPA PAPAPAPAP APAPQLLTE SVQKTFNVA FNWGKNVQT LPPPGKAAP VLIPVAVPA PAPAPAPAP APAPAPAPA PAPAPAPAP PAKPGAPPK PPSKVQLLR EYDEDYPAR LDLPEKVQI ELPDYSTDS GSEKREINY DYLHSKENF KEGFKTFWD NSPYTAEHG YKFLAPQAN SQENFQKDI PLSKYEEYD LKPKHHNSI RSVSTPKFF VNSHSLNID ETPLLEVHD VNGDLAKQN IELHRHILE YYKKKFAAD AETARRQAH QLAPPQKIT YQAVGPEKQ YLTLERLDK IIQRGRDNY YKKENLRNY GEEVEETPA PVLHSKNPE RENLKHYEE EVEEIPAPV FHSKNPERE NLKNYEEEV EEIPAPVFH LKNPERENL KNHKEENEE RSLKSASPK DDVEEISKT KKDEPKKEE EEEEIEEVP PTEFTKSLK FDPEKKFQE FKFLPPEDT SKEVKGVDR TIDDEISSN FQRNHKKYE DIKDDFLQK HEDKEEISE KLRKQPTPA PFKIVDVDE KENFDYEML PHLSEESKS EDIKEEKNL EKEEPHVED HDKQFENIR QEFLDREKQ YTGYTPEEK RRDSNEKEI IAFHDVDGN VYRENAEEG EEYEGQNED YDEGKKEVE QKAVNDHYA NYNRDDGNK FRIYFDYEN EEEKLPFYT GDIEGYVKH EFENDRNNL KQKTEQNLE NYYRSLEEG DPNQGEESE KVQQDCETD EEKEPENEE TKQEEEKQS LKKVKGFFV APTQAQNLA PNLYEGIDH ASAFLSIPN YFGEHQQAA PGFEHNFGK NFPVKQQQA ATQNSEVVQ PLYSGIISV GALQELDKS NKPLTIPFD FDYSASEIK VKKTKVAFD SKPAATEYT GYESHYVPE KYTEDFSYT VPEPPPVDY AKLEAKDFE DDDTLSKNA KILNLESGR TEAFLTING NKYHL
- >TC007307 RA
atgctcata aatcaacaa atctgttgt gttctcctg ttcctagtg actgtaaat agtgaaact agtgactcc agtgaacgt ggaaagaga acgataggc tcatggcta acaaacatc ggagacctt ttcggttat gacgtgcac aaacggccg tcaatggtg cggccccca gtgactgtc ctcacagcc gcagtgccc gcaaaccta atcgcagga ccggtcttc gcccgacag aaggcagtg ggcccggcg cctccgcca aaatcggcc cctgcacca gccccggcc ccggcccca gccccagca cctgcccca gctccggcc ccggccccg gccccagcc cccgcacca gccccagcc ccgcaacta ctaacagaa tcggtgcaa aaaacattc aatgtcgcc ttcaactgg ggaaagaac gtccagact ttaccacct ccggggaaa gcggccccg gttttaatt ccggttgct gtcccggct ccggcaccg gccccagcc ccggctccg gcgcccgct ccggcacct gcccccgct ccggcccca gctccggcg cccgcgcct ccggcgaaa cccggagcg cccccaaaa ccgccctcc aaagtccag ttactgcga gagtacgat gaggattat cccgcaagg ttagattta ccggaaaag gtccaaatc gaactcccg gattactcg actgactcc ggatcggaa aagcgtgaa attaattac gattatctc cattcgaaa gagaatttt aaagaagga tttaaaacg ttttgggat aattctcct tacaccgca gaacatggc tataagttt ctagcaccg caagcgaat tcgcaagaa aatttccaa aaagatatt ccattatcg aaatacgag gagtacgat ttgaaacct aagcaccat aacagtatt cgaagtgtt tcaactccg aaatttttt gttaattcc cacagtttg aacatcgat gagacgcca cttttggaa gttcacgac gttaatggt gatttagcc aaacaaaat attgagctt catagacac attttggaa tattataaa aaaaaattt gcagcggat gcggaaact gcacggcga caagcgcat caacttgcg cccccgcaa aaaatcacc taccaagct gttggcccc gaaaaacaa tacctgact ctagaacgg ctagataaa atcattcag agagggaga gataattac tacaaaaag gaaaacttg agaaattac ggggaagaa gttgaggaa actcccgcc ccggttttg cactcgaaa aatcccgaa agagaaaac ttgaaacat tacgaggaa gaagttgag gaaattccc gctccggtt ttccactcg aaaaatccc gaaagagaa aacttgaaa aattacgag gaagaagtt gaggaaatt cctgctccg gtgttccat ttgaaaaat cctgaaaga gaaaacttg aaaaaccat aaagaagaa aatgaagaa cgatctttg aaaagtgcg tcaccgaag gatgatgtt gaagagatc agcaaaacg aagaaagat gagccaaaa aaagaggag gaagaggaa gaaattgaa gaagtgcca ccgaccgaa ttcaccaag agtctcaaa ttcgatcct gaaaaaaag tttcaggag tttaagttt cttccccct gaagacacg agtaaagag gttaagggc gttgacagg accatcgat gatgaaata tcctcaaat tttcaaaga aatcacaag aaatacgaa gacataaag gacgatttt ttgcaaaaa catgaagat aaggaggag ataagtgag aaattgagg aagcaacca acccctgcg ccttttaaa atcgtggac gttgacgag aaggaaaat tttgactac gaaatgttg ccacacttg agcgaagaa agcaaaagt gaagacata aaggaagag aaaaatttg gagaaggag gagcctcat gttgaagac cacgacaaa cagtttgaa aatattcgg caagaattt ctagatcga gaaaagcag tacacggga tatacgccc gaagaaaaa cgccgggat tccaacgag aaagaaata atcgcattc catgacgtt gatggaaat gtttatcgc gaaaatgcg gaagaaggg gaagaatac gaaggccag aatgaagat tacgacgag ggcaaaaaa gaagtggaa cagaaagcc gtcaacgat cattacgct aattacaac cgagacgat ggaaataaa ttccgaatt tacttcgat tacgaaaac gaagaagaa aaactgcct ttctacacc ggagacatt gagggttac gtaaaacac gaattcgaa aacgaccga aataacttg aagcaaaaa acggaacaa aacctcgaa aactactac cgcagtctg gaggaaggg gaccccaac cagggcgaa gagagtgaa aaggtgcaa caagactgc gaaacagac gaagaaaaa gaaccagaa aatgaagaa acgaaacaa gaagaagaa aaacagtcc ttgaagaaa gtcaaggga tttttcgta gcaccgaca caagcccag aacttggct ccgaattta tacgaaggg atcgaccac gctagtgcc tttctatca attcctaat tacttcggc gagcatcag caggcagcc cccggattt gagcacaat ttcgggaag aatttcccc gtgaagcaa caacaagcc gccacgcaa aattccgaa gtcgttcag ccgctctac tccggtatt attagcgtg ggggcgttg caagagctg gacaagtct aacaaaccg ttgacgatt ccgttcgat tttgattac agcgcgtct gaaatcaag gtgaagaag acgaaagtc gcctttgat agtaaacca gccgcgacg gaatataca gggtatgag agtcattat gtgcctgag aaatataca gaggacttt tcttatact gttccagaa ccgccaccg gttgattac gcgaaactc gaagctaaa gatttcgag gatgatgat acgctttcg aaaaatgct aaaatttta aatttggag tcggggaga acggaggct tttttgacc attaacggg aacaagtat catctctag
- >TC007307 RA
aagagcatg gcacaatgc tcataaatc aacaaatct gttgtgttc tcctgttcc tagtgactg taaatagtg aaactagtg actccagtg aacgtggaa agagaacga taggctcat ggctaacaa acatcggag accttttcg gttatgacg tgcacaaac ggccgtcaa tggtgcggc ccccagtga ctgtcctca cagccgcag tgcccgcaa acctaatcg caggaccgg tcttcgccc gacagaagg cagtgggcc cggcgcctc cgccaaaat cggcccctg caccagccc cggccccgg ccccagccc cagcacctg ccccagctc cggccccgg ccccggccc cagcccccg caccagccc cagccccgc aactactaa cagaatcgg tgcaaaaaa cattcaatg tcgccttca actggggaa agaacgtcc agactttac cacctccgg ggaaagcgg ccccggttt taattccgg ttgctgtcc cggctccgg caccggccc cagccccgg ctccggcgc ccgctccgg cacctgccc ccgctccgg ccccagctc cggcgcccg cgcctccgg cgaaacccg gagcgcccc caaaaccgc cctccaaag tccagttac tgcgagagt acgatgagg attatcccg caaggttag atttaccgg aaaaggtcc aaatcgaac tcccggatt actcgactg actccggat cggaaaagc gtgaaatta attacgatt atctccatt cgaaagaga attttaaag aaggattta aaacgtttt gggataatt ctccttaca ccgcagaac atggctata agtttctag caccgcaag cgaattcgc aagaaaatt tccaaaaag atattccat tatcgaaat acgaggagt acgatttga aacctaagc accataaca gtattcgaa gtgtttcaa ctccgaaat tttttgtta attcccaca gtttgaaca tcgatgaga cgccacttt tggaagttc acgacgtta atggtgatt tagccaaac aaaatattg agcttcata gacacattt tggaatatt ataaaaaaa aatttgcag cggatgcgg aaactgcac ggcgacaag cgcatcaac ttgcgcccc cgcaaaaaa tcacctacc aagctgttg gccccgaaa aacaatacc tgactctag aacggctag ataaaatca ttcagagag ggagagata attactaca aaaaggaaa acttgagaa attacgggg aagaagttg aggaaactc ccgccccgg ttttgcact cgaaaaatc ccgaaagag aaaacttga aacattacg aggaagaag ttgaggaaa ttcccgctc cggttttcc actcgaaaa atcccgaaa gagaaaact tgaaaaatt acgaggaag aagttgagg aaattcctg ctccggtgt tccatttga aaaatcctg aaagagaaa acttgaaaa accataaag aagaaaatg aagaacgat ctttgaaaa gtgcgtcac cgaaggatg atgttgaag agatcagca aaacgaaga aagatgagc caaaaaaag aggaggaag aggaagaaa ttgaagaag tgccaccga ccgaattca ccaagagtc tcaaattcg atcctgaaa aaaagtttc aggagttta agtttcttc cccctgaag acacgagta aagaggtta agggcgttg acaggacca tcgatgatg aaatatcct caaattttc aaagaaatc acaagaaat acgaagaca taaaggacg attttttgc aaaaacatg aagataagg aggagataa gtgagaaat tgaggaagc aaccaaccc ctgcgcctt ttaaaatcg tggacgttg acgagaagg aaaattttg actacgaaa tgttgccac acttgagcg aagaaagca aaagtgaag acataaagg aagagaaaa atttggaga aggaggagc ctcatgttg aagaccacg acaaacagt ttgaaaata ttcggcaag aatttctag atcgagaaa agcagtaca cgggatata cgcccgaag aaaaacgcc gggattcca acgagaaag aaataatcg cattccatg acgttgatg gaaatgttt atcgcgaaa atgcggaag aaggggaag aatacgaag gccagaatg aagattacg acgagggca aaaaagaag tggaacaga aagccgtca acgatcatt acgctaatt acaaccgag acgatggaa ataaattcc gaatttact tcgattacg aaaacgaag aagaaaaac tgcctttct acaccggag acattgagg gttacgtaa aacacgaat tcgaaaacg accgaaata acttgaagc aaaaaacgg aacaaaacc tcgaaaact actaccgca gtctggagg aaggggacc ccaaccagg gcgaagaga gtgaaaagg tgcaacaag actgcgaaa cagacgaag aaaaagaac cagaaaatg aagaaacga aacaagaag aagaaaaac agtccttga agaaagtca agggatttt tcgtagcac cgacacaag cccagaact tggctccga atttatacg aagggatcg accacgcta gtgcctttc tatcaattc ctaattact tcggcgagc atcagcagg cagcccccg gatttgagc acaatttcg ggaagaatt tccccgtga agcaacaac aagccgcca cgcaaaatt ccgaagtcg ttcagccgc tctactccg gtattatta gcgtggggg cgttgcaag agctggaca agtctaaca aaccgttga cgattccgt tcgattttg attacagcg cgtctgaaa tcaaggtga agaagacga aagtcgcct ttgatagta aaccagccg cgacggaat atacagggt atgagagtc attatgtgc ctgagaaat atacagagg acttttctt atactgttc cagaaccgc caccggttg attacgcga aactcgaag ctaaagatt tcgaggatg atgatacgc tttcgaaaa atgctaaaa ttttaaatt tggagtcgg ggagaacgg aggcttttt tgaccatta acgggaaca agtatcatc tctaggaat cggtagaaa atcatagtc taatttatt attacacgt gaccaaata gcgttatgt tgaaaataa gtttttata ttgttgcac catttgtta aaaaattat gtaaataac tacaaagat cttacttat ttaatgaca ctcattatc gtgcttttt aagtttata ttattgctt gttaactat ttgtataat gttggtatt aataaagtt taaaacata ag
