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Gene information
General information
TC007299 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6383065..6384457
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007299
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06233

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007299 RA
      MVTVFNLAQ WRPQRPEIV DLPAWRPIE NQDYEYSTT 
SGYSHNTYH GPTRHCRPM LTGSQLITL QHEEQRPLR 
FTDSPQHHP PNPSPRRKS SSTSNDGSE HYPPFDRTA 
TIYVPGDYQ TDPPHCHGC SAPGYHYGG HYPDHYAGY 
SSEYSCTTM YGYGTDYAY DNPSSISSH SGSRTPQRL 
PQGHRRTPS NVSNSSTTT SGSNVNPSF KLEDEGSYT 
SGYLRRYDY DYSAYSRQN SQESNFERP TTLETSGFT 
KLRSSLKRN NYQASQGHS GGNTPTNPT PPDSLTSDD 
SSYVSAKDS NSSVSRVRF SPTTLIDLP VPGQNQDPT 
VPLQARRSR QKQADKEFW S
    CDS sequence
    • >TC007299 RA
      atggttact gttttcaac ctagctcag tggcgcccc 
caacggccg gaaatcgtc gacctcccc gcttggaga 
cccatcgag aaccaagac tacgaatac tccacaact 
tccggctac agtcacaac acctaccac ggccccacc 
cgccactgc cgccccatg ttgaccggt tcccagctc 
atcaccctc cagcacgaa gagcaacgt cctttgcgc 
ttcaccgac tcaccccaa caccaccct cccaaccct 
tcccctcgc cgaaaatca agctcgaca agcaacgac 
ggctccgaa cactacccc cctttcgac cgaactgcc 
acgatttac gttccgggc gactaccaa actgacccc 
cctcactgt cacgggtgc tccgccccc ggctatcac 
tacgggggc cactacccg gaccactac gcagggtac 
agttccgag tacagttgc accaccatg tacggttac 
ggcactgat tacgcttac gataacccg agcagtata 
agtagccac agtggctca agaaccccc caaagactg 
ccgcagggg cacagacgg accccgtcc aatgtctca 
aacagctcg actacgaca agtggctca aacgtcaac 
ccgagcttc aagctggaa gatgagggg agttacaca 
tcgggttac ctgcggcgg tacgactac gactacagc 
gcctactcg cggcagaac tcgcaagag tcgaacttc 
gagcgtcca accacgttg gagacttcg ggttttacg 
aagttacgc tcgagtttg aagcggaat aattaccag 
gcgagtcag ggacactct ggggggaat accccgacg 
aatccaacg ccgcctgat agtctgacg agtgacgat 
agttcgtac gtttcggcg aaggatagt aatagttct 
gtaagtcgc gttaggttt tcgccaacg acgctcatt 
gatttgccc gttccgggg caaaatcag gacccgacg 
gtgccgctg caagcgagg aggtcgcgg cagaagcag 
gcggataag gagttttgg tcataa
    mRNA sequence
    • >TC007299 RA
      atagacaat gtaataatt gtgaagccc caaaattcg 
tacaacgct caaaatatc cgaataaac attttcctg 
ccatttatg gttactgtt ttcaaccta gctcagtgg 
cgcccccaa cggccggaa atcgtcgac ctccccgct 
tggagaccc atcgagaac caagactac gaatactcc 
acaacttcc ggctacagt cacaacacc taccacggc 
cccacccgc cactgccgc cccatgttg accggttcc 
cagctcatc accctccag cacgaagag caacgtcct 
ttgcgcttc accgactca ccccaacac caccctccc 
aacccttcc cctcgccga aaatcaagc tcgacaagc 
aacgacggc tccgaacac tacccccct ttcgaccga 
actgccacg atttacgtt ccgggcgac taccaaact 
gacccccct cactgtcac gggtgctcc gcccccggc 
tatcactac gggggccac tacccggac cactacgca 
gggtacagt tccgagtac agttgcacc accatgtac 
ggttacggc actgattac gcttacgat aacccgagc 
agtataagt agccacagt ggctcaaga accccccaa 
agactgccg caggggcac agacggacc ccgtccaat 
gtctcaaac agctcgact acgacaagt ggctcaaac 
gtcaacccg agcttcaag ctggaagat gaggggagt 
tacacatcg ggttacctg cggcggtac gactacgac 
tacagcgcc tactcgcgg cagaactcg caagagtcg 
aacttcgag cgtccaacc acgttggag acttcgggt 
tttacgaag ttacgctcg agtttgaag cggaataat 
taccaggcg agtcaggga cactctggg gggaatacc 
ccgacgaat ccaacgccg cctgatagt ctgacgagt 
gacgatagt tcgtacgtt tcggcgaag gatagtaat 
agttctgta agtcgcgtt aggttttcg ccaacgacg 
ctcattgat ttgcccgtt ccggggcaa aatcaggac 
ccgacggtg ccgctgcaa gcgaggagg tcgcggcag 
aagcaggcg gataaggag ttttggtca taacctttg 
tggcttacg taattttca gacttgttt aagggttga 
atcggttac aattgaaat tattgttag caatattga 
gtatttaat actagccaa taaagtgat ttgtgtgat 
gttgggcgg gaagggggc gccaacact tcgaagctt 
gggataaat gagaaatcg ctttcaagt ttaaaacat 
gcgaagcca gtcaagcgt tcattttgt ttaaaagaa 
aaaactggg tggaaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY