Status
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Gene information
General information
TC031111
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007243,TC007244
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6992841..7058697
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031111
    iBeetle screen iB_06232

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031111 RA
      MPITQLLGT KDTDESCRT FILQGEGHT LGNALRCVI 
      SGYPDVEFC AYTVPHPME CSMHLRIQM RSGRAVDAL 
      KRGLEDLAA LSDHILDEF TREKQNFDS RDELGYRLF 
      KVLGEGSYA KVYLAEYKG TAGDEKNKP RQLACKIVD 
      TTKAPRDFV KKFLPRELD ILVKINHPH LIHVHSIFQ 
      RKSKYFIFM RYAENGDLL EFILKKGSI SEAQSRVWM 
      RQLALAVQY LHDMEIAHR DLKCENALI TSNYNLKVS 
      DFGFARYVT DAYGKKLTS DTYCGSLSY AAPEILKGS 
      PYHPKIADI WSLGVVLYI MLNKAMPFD DTNIKRLHE 
      QQMNRQWKF RSKVVDVIS SEVKRLMSH LLEPDTSKR 
      WKIEQILAS DWFGMDPRL AQLNPAEQA AMVHAQEER 
      KKYMDNLRK KSGPKAIKG DGSKEVSVL KKEVETQET 
      NKLATMSLP SMIAED
    CDS sequence
    • >TC031111 RA
      atgccaatc acacagctc ctgggaacc aaagacaca 
      gacgaatcc tgccgaacg ttcattcta caaggcgag 
      ggtcacact ttaggcaac gctctcaga tgtgtcatt 
      tctggctat cctgacgtc gaattttgc gcttacaca 
      gtcccccat cccatggaa tgttcaatg cacctccgc 
      attcagatg cgctcaggc cgcgctgtg gacgccctc 
      aaacgaggc ttggaggac ctggcagcc ctctccgac 
      cacattctg gacgagttc acacgcgag aaacaaaac 
      ttcgacagt agagacgaa ttaggctac cgactgttc 
      aaagtcctc ggtgagggc tcctatgct aaggtgtac 
      cttgcggag tacaagggc accgccggg gacgagaaa 
      aacaagccc cggcaattg gcctgcaaa atagtcgac 
      acaacgaaa gccccccgc gattttgtc aagaagttc 
      ctgccgcgc gagctggat attttggtc aaaatcaat 
      cacccgcac ttgatacac gtccacagc atttttcag 
      cgcaaatca aagtacttt attttcatg aggtatgcg 
      gaaaacggc gacttgctc gagtttatt ttgaaaaaa 
      ggctcgatt tcggaggca cagtcgcgg gtttggatg 
      cgacagcta gccctagct gtgcaatat ttgcacgat 
      atggagata gcgcaccgg gatttaaaa tgtgaaaat 
      gccttaatc accagtaat tataactta aaagtgtcc 
      gatttcgga ttcgcgaga tacgtgaca gacgcctac 
      ggcaaaaaa ctgaccagt gacacgtac tgcgggtct 
      ttgagctac gcagccccc gaaatcctc aaaggctcg 
      ccctatcac cccaaaatc gccgatatt tggtcactt 
      ggagttgtc ttatacatc atgcttaac aaagccatg 
      cctttcgac gacacaaat atcaaaaga ctccacgag 
      caacaaatg aaccgacag tggaaattc cggtcgaaa 
      gtcgtcgat gtgataagt tcggaggtg aagcgtctc 
      atgagccac ctcctagag ccggacacg tccaagcgg 
      tggaagatc gagcagatt ttggcgtcg gattggttc 
      gggatggac ccacggctg gcccagttg aacccagcg 
      gaacaggcg gccatggtc catgcccag gaagagcgt 
      aaaaaatat atggacaat ctgaggaag aaatcggga 
      cctaaagcc atcaaaggc gacggttcg aaagaagtt 
      tcggtgttg aaaaaagaa gtcgagacg caggagacc 
      aacaagttg gccacgatg agtctcccc agcatgatt 
      gcggaggat tga
    mRNA sequence
    • >TC031111 RA
      actgaaata agaaaataa tgccgcaaa agataaaag 
      acctaaccc cacttaaaa aatgccaat cacacagct 
      cctgggaac caaagacac agacgaatc ctgccgaac 
      gttcattct acaaggcga gggtcacac tttaggcaa 
      cgctctcag atgtgtcat ttctggcta tcctgacgt 
      cgaattttg cgcttacac agtccccca tcccatgga 
      atgttcaat gcacctccg cattcagat gcgctcagg 
      ccgcgctgt ggacgccct caaacgagg cttggagga 
      cctggcagc cctctccga ccacattct ggacgagtt 
      cacacgcga gaaacaaaa cttcgacag tagagacga 
      attaggcta ccgactgtt caaagtcct cggtgaggg 
      ctcctatgc taaggtgta ccttgcgga gtacaaggg 
      caccgccgg ggacgagaa aaacaagcc ccggcaatt 
      ggcctgcaa aatagtcga cacaacgaa agccccccg 
      cgattttgt caagaagtt cctgccgcg cgagctgga 
      tattttggt caaaatcaa tcacccgca cttgataca 
      cgtccacag catttttca gcgcaaatc aaagtactt 
      tattttcat gaggtatgc ggaaaacgg cgacttgct 
      cgagtttat tttgaaaaa aggctcgat ttcggaggc 
      acagtcgcg ggtttggat gcgacagct agccctagc 
      tgtgcaata tttgcacga tatggagat agcgcaccg 
      ggatttaaa atgtgaaaa tgccttaat caccagtaa 
      ttataactt aaaagtgtc cgatttcgg attcgcgag 
      atacgtgac agacgccta cggcaaaaa actgaccag 
      tgacacgta ctgcgggtc tttgagcta cgcagcccc 
      cgaaatcct caaaggctc gccctatca ccccaaaat 
      cgccgatat ttggtcact tggagttgt cttatacat 
      catgcttaa caaagccat gcctttcga cgacacaaa 
      tatcaaaag actccacga gcaacaaat gaaccgaca 
      gtggaaatt ccggtcgaa agtcgtcga tgtgataag 
      ttcggaggt gaagcgtct catgagcca cctcctaga 
      gccggacac gtccaagcg gtggaagat cgagcagat 
      tttggcgtc ggattggtt cgggatgga cccacggct 
      ggcccagtt gaacccagc ggaacaggc ggccatggt 
      ccatgccca ggaagagcg taaaaaata tatggacaa 
      tctgaggaa gaaatcggg acctaaagc catcaaagg 
      cgacggttc gaaagaagt ttcggtgtt gaaaaaaga 
      agtcgagac gcaggagac caacaagtt ggccacgat 
      gagtctccc cagcatgat tgcggagga ttgaaaaaa 
      cgaacagtc cgatagttt tatgtggta ataaatatt 
      taaaatgag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY