Status
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Gene information
General information
TC007083 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9898641..9922490
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007083
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06228

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007083 RA
      MMWLDEGKR RVLGDSDDD SVSSGEEVP IWVRGEQRW 
      VSGITGETT CQDVIQVLL HDEESRGRH IGLPEQYHI 
      TERWRGVEQ PLDRQSVIL DIWNAWGTA QPEVKISLR 
      RIKYDRPPS RRTRCRSDS STWSDRSAW KTIHPKRLH 
      ALQNEKQPS STEELLKLV LAQGEVIRR QLKKLRHSE 
      HQIGYLEDK THKARVRKH GSNYLLETY LKGLSEAVD 
      PENDANLCA DKNSDSGVM TEGDSEQSN TKSRPAEQT 
      FERFSPSSE EFSKDIKDD DTSSTVSEA TIREQIELF 
      ESIAKLNKR LLKEEESLV RLDANLKKY DKHKTDDLA 
      KALTNLRTE MTKSACEMQ HNEVVLEET MEMLLNRRH 
      YLENLHRDL ANEEQEHEM LQALMFSTR RQRHNPDSC 
      SPYYSHTKE LLDTLV
    • >TC007083 RB
      MFYIITASR VEVNAPILS NNFISDTMM WLDEGKRRV 
      LGDSDDDSV SSGEEVPIW VRGEQRWVS GITGETTCQ 
      DVIQVLLHD EESRGRHIG LPEQYHITE RWRGVEQPL 
      DRQSVILDI WNAWGTAQP EVKISLRRI KYDRPPSRR 
      TRCRSDSST WSDRSAWKT IHPKRLHAL QNEKQPSST 
      EELLKLVLA QGEVIRRQL KKLRHSEHQ IGYLEDKTH 
      KARVRKHGS NYLLETYLK GLSEAVDPE NDANLCADK 
      NSDSGVMTE GDSEQSNTK SRPAEQTFE RFSPSSEEF 
      SKDIKDDDT SSTVSEATI REQIELFES IAKLNKRLL 
      KEEESLVRL DANLKKYDK HKTDDLAKA LTNLRTEMT 
      KSACEMQHN EVVLEETME MLLNRRHYL ENLHRDLAN 
      EEQEHEMLQ ALMFSTRRQ RHNPDSCSP YYSHTKELL 
      DTLV
    CDS sequence
    • >TC007083 RA
      atgatgtgg ttggatgaa ggcaagaga cgcgtcttg 
      ggcgactcg gacgacgac agtgtcagc agtggcgag 
      gaggtgccc atatgggtg cgaggcgag cagagatgg 
      gtctctggc atcaccggc gagaccacg tgccaggac 
      gtcatccag gtgctcctc cacgacgag gagagcagg 
      ggccgccac atcggtttg ccagaacag taccatata 
      acagagcgg tggagaggc gtggagcag cctttagac 
      cgccagtct gtcatttta gacatttgg aatgcttgg 
      ggaacagca cagcctgaa gtcaaaata tcactcaga 
      cgcatcaaa tatgacagg ccaccaagt cgaagaacc 
      cgctgccgg tccgactcg agcacttgg tcagaccgc 
      agcgcttgg aagactatc cacccgaag aggctccac 
      gcgttacag aacgaaaaa caaccatca agtacggaa 
      gaattactg aaactagtt ctagctcaa ggagaagtt 
      ataagacga caattaaag aagttgaga cacagcgaa 
      caccaaatc ggctattta gaggataag acgcacaag 
      gcccgcgtg cgcaaacac ggcagcaac tacctcctg 
      gagacgtac ctaaagggc ttatccgag gctgtagac 
      cccgaaaat gacgccaat ttgtgcgct gacaaaaac 
      agcgacagt ggagtcatg actgaaggc gacagcgag 
      cagtctaat accaagtcg agaccagcg gaacagacg 
      tttgagcgg ttttctccc tcgtccgaa gagttcagc 
      aaagacata aaagacgac gacacgagc tccacagtg 
      agcgaagcg acgatcagg gaacaaatc gaattattc 
      gagtctata gcgaaattg aacaaacgt cttctcaag 
      gaagaagag agtttagtt aggctagat gcgaatcta 
      aagaagtac gataagcac aaaacggac gacttggcg 
      aaagcactc actaatttg aggacggaa atgactaaa 
      agtgcctgt gaaatgcaa cataatgag gtggttctc 
      gaggaaacg atggagatg ttgcttaat cggcggcat 
      tacctggag aatcttcac agggacctt gctaatgaa 
      gagcaggag cacgagatg ctgcaagcg ctgatgttc 
      agcaccagg aggcagagg cacaacccg gacagttgt 
      agtccctac tactcacat accaaagaa ttgttagat 
      accttagtt tag
    • >TC007083 RB
      atgttttac ataataacg gcatctcgc gtagaagta 
      aacgcgccc attttaagc aataacttt atttcagat 
      acgatgatg tggttggat gaaggcaag agacgcgtc 
      ttgggcgac tcggacgac gacagtgtc agcagtggc 
      gaggaggtg cccatatgg gtgcgaggc gagcagaga 
      tgggtctct ggcatcacc ggcgagacc acgtgccag 
      gacgtcatc caggtgctc ctccacgac gaggagagc 
      aggggccgc cacatcggt ttgccagaa cagtaccat 
      ataacagag cggtggaga ggcgtggag cagccttta 
      gaccgccag tctgtcatt ttagacatt tggaatgct 
      tggggaaca gcacagcct gaagtcaaa atatcactc 
      agacgcatc aaatatgac aggccacca agtcgaaga 
      acccgctgc cggtccgac tcgagcact tggtcagac 
      cgcagcgct tggaagact atccacccg aagaggctc 
      cacgcgtta cagaacgaa aaacaacca tcaagtacg 
      gaagaatta ctgaaacta gttctagct caaggagaa 
      gttataaga cgacaatta aagaagttg agacacagc 
      gaacaccaa atcggctat ttagaggat aagacgcac 
      aaggcccgc gtgcgcaaa cacggcagc aactacctc 
      ctggagacg tacctaaag ggcttatcc gaggctgta 
      gaccccgaa aatgacgcc aatttgtgc gctgacaaa 
      aacagcgac agtggagtc atgactgaa ggcgacagc 
      gagcagtct aataccaag tcgagacca gcggaacag 
      acgtttgag cggttttct ccctcgtcc gaagagttc 
      agcaaagac ataaaagac gacgacacg agctccaca 
      gtgagcgaa gcgacgatc agggaacaa atcgaatta 
      ttcgagtct atagcgaaa ttgaacaaa cgtcttctc 
      aaggaagaa gagagttta gttaggcta gatgcgaat 
      ctaaagaag tacgataag cacaaaacg gacgacttg 
      gcgaaagca ctcactaat ttgaggacg gaaatgact 
      aaaagtgcc tgtgaaatg caacataat gaggtggtt 
      ctcgaggaa acgatggag atgttgctt aatcggcgg 
      cattacctg gagaatctt cacagggac cttgctaat 
      gaagagcag gagcacgag atgctgcaa gcgctgatg 
      ttcagcacc aggaggcag aggcacaac ccggacagt 
      tgtagtccc tactactca cataccaaa gaattgtta 
      gatacctta gtttag
    mRNA sequence
    • >TC007083 RA
      tttcagtca tcagtggtt cacccacca gtctttgcc 
      cgactgttg tagtgagtg ttctagcga tttttgagc 
      gctctgaca tgtggattt acctatgaa atcgtgtgc 
      tttgtgaat tttccgata cgatgatgt ggttggatg 
      aaggcaaga gacgcgtct tgggcgact cggacgacg 
      acagtgtca gcagtggcg aggaggtgc ccatatggg 
      tgcgaggcg agcagagat gggtctctg gcatcaccg 
      gcgagacca cgtgccagg acgtcatcc aggtgctcc 
      tccacgacg aggagagca ggggccgcc acatcggtt 
      tgccagaac agtaccata taacagagc ggtggagag 
      gcgtggagc agcctttag accgccagt ctgtcattt 
      tagacattt ggaatgctt ggggaacag cacagcctg 
      aagtcaaaa tatcactca gacgcatca aatatgaca 
      ggccaccaa gtcgaagaa cccgctgcc ggtccgact 
      cgagcactt ggtcagacc gcagcgctt ggaagacta 
      tccacccga agaggctcc acgcgttac agaacgaaa 
      aacaaccat caagtacgg aagaattac tgaaactag 
      ttctagctc aaggagaag ttataagac gacaattaa 
      agaagttga gacacagcg aacaccaaa tcggctatt 
      tagaggata agacgcaca aggcccgcg tgcgcaaac 
      acggcagca actacctcc tggagacgt acctaaagg 
      gcttatccg aggctgtag accccgaaa atgacgcca 
      atttgtgcg ctgacaaaa acagcgaca gtggagtca 
      tgactgaag gcgacagcg agcagtcta ataccaagt 
      cgagaccag cggaacaga cgtttgagc ggttttctc 
      cctcgtccg aagagttca gcaaagaca taaaagacg 
      acgacacga gctccacag tgagcgaag cgacgatca 
      gggaacaaa tcgaattat tcgagtcta tagcgaaat 
      tgaacaaac gtcttctca aggaagaag agagtttag 
      ttaggctag atgcgaatc taaagaagt acgataagc 
      acaaaacgg acgacttgg cgaaagcac tcactaatt 
      tgaggacgg aaatgacta aaagtgcct gtgaaatgc 
      aacataatg aggtggttc tcgaggaaa cgatggaga 
      tgttgctta atcggcggc attacctgg agaatcttc 
      acagggacc ttgctaatg aagagcagg agcacgaga 
      tgctgcaag cgctgatgt tcagcacca ggaggcaga 
      ggcacaacc cggacagtt gtagtccct actactcac 
      ataccaaag aattgttag ataccttag tttagcgct 
      tttaatccc taattaatt taataccaa atatcttgt 
      acctaattc acgttggaa atttgttag tcattgatc 
      tgaaatata tgtttttta caaac
    • >TC007083 RB
      tttcagtca tcagtggtt cacccacca gtctttgcc 
      cgactgttg tagtgagtg ttctagcga tttttgagc 
      gctctgaca tgtggattt acctatgaa atcgtgtgc 
      tttgtgaat tttccggtt tgagagagc tatagcaag 
      cttggtaag tagtgtttt tttcagtga tgttttaca 
      taataacgg catctcgcg tagaagtaa acgcgccca 
      ttttaagca ataacttta tttcagata cgatgatgt 
      ggttggatg aaggcaaga gacgcgtct tgggcgact 
      cggacgacg acagtgtca gcagtggcg aggaggtgc 
      ccatatggg tgcgaggcg agcagagat gggtctctg 
      gcatcaccg gcgagacca cgtgccagg acgtcatcc 
      aggtgctcc tccacgacg aggagagca ggggccgcc 
      acatcggtt tgccagaac agtaccata taacagagc 
      ggtggagag gcgtggagc agcctttag accgccagt 
      ctgtcattt tagacattt ggaatgctt ggggaacag 
      cacagcctg aagtcaaaa tatcactca gacgcatca 
      aatatgaca ggccaccaa gtcgaagaa cccgctgcc 
      ggtccgact cgagcactt ggtcagacc gcagcgctt 
      ggaagacta tccacccga agaggctcc acgcgttac 
      agaacgaaa aacaaccat caagtacgg aagaattac 
      tgaaactag ttctagctc aaggagaag ttataagac 
      gacaattaa agaagttga gacacagcg aacaccaaa 
      tcggctatt tagaggata agacgcaca aggcccgcg 
      tgcgcaaac acggcagca actacctcc tggagacgt 
      acctaaagg gcttatccg aggctgtag accccgaaa 
      atgacgcca atttgtgcg ctgacaaaa acagcgaca 
      gtggagtca tgactgaag gcgacagcg agcagtcta 
      ataccaagt cgagaccag cggaacaga cgtttgagc 
      ggttttctc cctcgtccg aagagttca gcaaagaca 
      taaaagacg acgacacga gctccacag tgagcgaag 
      cgacgatca gggaacaaa tcgaattat tcgagtcta 
      tagcgaaat tgaacaaac gtcttctca aggaagaag 
      agagtttag ttaggctag atgcgaatc taaagaagt 
      acgataagc acaaaacgg acgacttgg cgaaagcac 
      tcactaatt tgaggacgg aaatgacta aaagtgcct 
      gtgaaatgc aacataatg aggtggttc tcgaggaaa 
      cgatggaga tgttgctta atcggcggc attacctgg 
      agaatcttc acagggacc ttgctaatg aagagcagg 
      agcacgaga tgctgcaag cgctgatgt tcagcacca 
      ggaggcaga ggcacaacc cggacagtt gtagtccct 
      actactcac ataccaaag aattgttag ataccttag 
      tttagcgct tttaatccc taattaatt taataccaa 
      atatcttgt acctaattc acgttggaa atttgttag 
      tcattgatc tgaaatata tgtttttta caaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY