Status
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Gene information
General information
TC007083 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9898641..9922490
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007083
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06228

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007083 RA
      MMWLDEGKR RVLGDSDDD SVSSGEEVP IWVRGEQRW 
VSGITGETT CQDVIQVLL HDEESRGRH IGLPEQYHI 
TERWRGVEQ PLDRQSVIL DIWNAWGTA QPEVKISLR 
RIKYDRPPS RRTRCRSDS STWSDRSAW KTIHPKRLH 
ALQNEKQPS STEELLKLV LAQGEVIRR QLKKLRHSE 
HQIGYLEDK THKARVRKH GSNYLLETY LKGLSEAVD 
PENDANLCA DKNSDSGVM TEGDSEQSN TKSRPAEQT 
FERFSPSSE EFSKDIKDD DTSSTVSEA TIREQIELF 
ESIAKLNKR LLKEEESLV RLDANLKKY DKHKTDDLA 
KALTNLRTE MTKSACEMQ HNEVVLEET MEMLLNRRH 
YLENLHRDL ANEEQEHEM LQALMFSTR RQRHNPDSC 
SPYYSHTKE LLDTLV
    • >TC007083 RB
      MFYIITASR VEVNAPILS NNFISDTMM WLDEGKRRV 
LGDSDDDSV SSGEEVPIW VRGEQRWVS GITGETTCQ 
DVIQVLLHD EESRGRHIG LPEQYHITE RWRGVEQPL 
DRQSVILDI WNAWGTAQP EVKISLRRI KYDRPPSRR 
TRCRSDSST WSDRSAWKT IHPKRLHAL QNEKQPSST 
EELLKLVLA QGEVIRRQL KKLRHSEHQ IGYLEDKTH 
KARVRKHGS NYLLETYLK GLSEAVDPE NDANLCADK 
NSDSGVMTE GDSEQSNTK SRPAEQTFE RFSPSSEEF 
SKDIKDDDT SSTVSEATI REQIELFES IAKLNKRLL 
KEEESLVRL DANLKKYDK HKTDDLAKA LTNLRTEMT 
KSACEMQHN EVVLEETME MLLNRRHYL ENLHRDLAN 
EEQEHEMLQ ALMFSTRRQ RHNPDSCSP YYSHTKELL 
DTLV
    CDS sequence
    • >TC007083 RA
      atgatgtgg ttggatgaa ggcaagaga cgcgtcttg 
ggcgactcg gacgacgac agtgtcagc agtggcgag 
gaggtgccc atatgggtg cgaggcgag cagagatgg 
gtctctggc atcaccggc gagaccacg tgccaggac 
gtcatccag gtgctcctc cacgacgag gagagcagg 
ggccgccac atcggtttg ccagaacag taccatata 
acagagcgg tggagaggc gtggagcag cctttagac 
cgccagtct gtcatttta gacatttgg aatgcttgg 
ggaacagca cagcctgaa gtcaaaata tcactcaga 
cgcatcaaa tatgacagg ccaccaagt cgaagaacc 
cgctgccgg tccgactcg agcacttgg tcagaccgc 
agcgcttgg aagactatc cacccgaag aggctccac 
gcgttacag aacgaaaaa caaccatca agtacggaa 
gaattactg aaactagtt ctagctcaa ggagaagtt 
ataagacga caattaaag aagttgaga cacagcgaa 
caccaaatc ggctattta gaggataag acgcacaag 
gcccgcgtg cgcaaacac ggcagcaac tacctcctg 
gagacgtac ctaaagggc ttatccgag gctgtagac 
cccgaaaat gacgccaat ttgtgcgct gacaaaaac 
agcgacagt ggagtcatg actgaaggc gacagcgag 
cagtctaat accaagtcg agaccagcg gaacagacg 
tttgagcgg ttttctccc tcgtccgaa gagttcagc 
aaagacata aaagacgac gacacgagc tccacagtg 
agcgaagcg acgatcagg gaacaaatc gaattattc 
gagtctata gcgaaattg aacaaacgt cttctcaag 
gaagaagag agtttagtt aggctagat gcgaatcta 
aagaagtac gataagcac aaaacggac gacttggcg 
aaagcactc actaatttg aggacggaa atgactaaa 
agtgcctgt gaaatgcaa cataatgag gtggttctc 
gaggaaacg atggagatg ttgcttaat cggcggcat 
tacctggag aatcttcac agggacctt gctaatgaa 
gagcaggag cacgagatg ctgcaagcg ctgatgttc 
agcaccagg aggcagagg cacaacccg gacagttgt 
agtccctac tactcacat accaaagaa ttgttagat 
accttagtt tag
    • >TC007083 RB
      atgttttac ataataacg gcatctcgc gtagaagta 
aacgcgccc attttaagc aataacttt atttcagat 
acgatgatg tggttggat gaaggcaag agacgcgtc 
ttgggcgac tcggacgac gacagtgtc agcagtggc 
gaggaggtg cccatatgg gtgcgaggc gagcagaga 
tgggtctct ggcatcacc ggcgagacc acgtgccag 
gacgtcatc caggtgctc ctccacgac gaggagagc 
aggggccgc cacatcggt ttgccagaa cagtaccat 
ataacagag cggtggaga ggcgtggag cagccttta 
gaccgccag tctgtcatt ttagacatt tggaatgct 
tggggaaca gcacagcct gaagtcaaa atatcactc 
agacgcatc aaatatgac aggccacca agtcgaaga 
acccgctgc cggtccgac tcgagcact tggtcagac 
cgcagcgct tggaagact atccacccg aagaggctc 
cacgcgtta cagaacgaa aaacaacca tcaagtacg 
gaagaatta ctgaaacta gttctagct caaggagaa 
gttataaga cgacaatta aagaagttg agacacagc 
gaacaccaa atcggctat ttagaggat aagacgcac 
aaggcccgc gtgcgcaaa cacggcagc aactacctc 
ctggagacg tacctaaag ggcttatcc gaggctgta 
gaccccgaa aatgacgcc aatttgtgc gctgacaaa 
aacagcgac agtggagtc atgactgaa ggcgacagc 
gagcagtct aataccaag tcgagacca gcggaacag 
acgtttgag cggttttct ccctcgtcc gaagagttc 
agcaaagac ataaaagac gacgacacg agctccaca 
gtgagcgaa gcgacgatc agggaacaa atcgaatta 
ttcgagtct atagcgaaa ttgaacaaa cgtcttctc 
aaggaagaa gagagttta gttaggcta gatgcgaat 
ctaaagaag tacgataag cacaaaacg gacgacttg 
gcgaaagca ctcactaat ttgaggacg gaaatgact 
aaaagtgcc tgtgaaatg caacataat gaggtggtt 
ctcgaggaa acgatggag atgttgctt aatcggcgg 
cattacctg gagaatctt cacagggac cttgctaat 
gaagagcag gagcacgag atgctgcaa gcgctgatg 
ttcagcacc aggaggcag aggcacaac ccggacagt 
tgtagtccc tactactca cataccaaa gaattgtta 
gatacctta gtttag
    mRNA sequence
    • >TC007083 RA
      tttcagtca tcagtggtt cacccacca gtctttgcc 
cgactgttg tagtgagtg ttctagcga tttttgagc 
gctctgaca tgtggattt acctatgaa atcgtgtgc 
tttgtgaat tttccgata cgatgatgt ggttggatg 
aaggcaaga gacgcgtct tgggcgact cggacgacg 
acagtgtca gcagtggcg aggaggtgc ccatatggg 
tgcgaggcg agcagagat gggtctctg gcatcaccg 
gcgagacca cgtgccagg acgtcatcc aggtgctcc 
tccacgacg aggagagca ggggccgcc acatcggtt 
tgccagaac agtaccata taacagagc ggtggagag 
gcgtggagc agcctttag accgccagt ctgtcattt 
tagacattt ggaatgctt ggggaacag cacagcctg 
aagtcaaaa tatcactca gacgcatca aatatgaca 
ggccaccaa gtcgaagaa cccgctgcc ggtccgact 
cgagcactt ggtcagacc gcagcgctt ggaagacta 
tccacccga agaggctcc acgcgttac agaacgaaa 
aacaaccat caagtacgg aagaattac tgaaactag 
ttctagctc aaggagaag ttataagac gacaattaa 
agaagttga gacacagcg aacaccaaa tcggctatt 
tagaggata agacgcaca aggcccgcg tgcgcaaac 
acggcagca actacctcc tggagacgt acctaaagg 
gcttatccg aggctgtag accccgaaa atgacgcca 
atttgtgcg ctgacaaaa acagcgaca gtggagtca 
tgactgaag gcgacagcg agcagtcta ataccaagt 
cgagaccag cggaacaga cgtttgagc ggttttctc 
cctcgtccg aagagttca gcaaagaca taaaagacg 
acgacacga gctccacag tgagcgaag cgacgatca 
gggaacaaa tcgaattat tcgagtcta tagcgaaat 
tgaacaaac gtcttctca aggaagaag agagtttag 
ttaggctag atgcgaatc taaagaagt acgataagc 
acaaaacgg acgacttgg cgaaagcac tcactaatt 
tgaggacgg aaatgacta aaagtgcct gtgaaatgc 
aacataatg aggtggttc tcgaggaaa cgatggaga 
tgttgctta atcggcggc attacctgg agaatcttc 
acagggacc ttgctaatg aagagcagg agcacgaga 
tgctgcaag cgctgatgt tcagcacca ggaggcaga 
ggcacaacc cggacagtt gtagtccct actactcac 
ataccaaag aattgttag ataccttag tttagcgct 
tttaatccc taattaatt taataccaa atatcttgt 
acctaattc acgttggaa atttgttag tcattgatc 
tgaaatata tgtttttta caaac
    • >TC007083 RB
      tttcagtca tcagtggtt cacccacca gtctttgcc 
cgactgttg tagtgagtg ttctagcga tttttgagc 
gctctgaca tgtggattt acctatgaa atcgtgtgc 
tttgtgaat tttccggtt tgagagagc tatagcaag 
cttggtaag tagtgtttt tttcagtga tgttttaca 
taataacgg catctcgcg tagaagtaa acgcgccca 
ttttaagca ataacttta tttcagata cgatgatgt 
ggttggatg aaggcaaga gacgcgtct tgggcgact 
cggacgacg acagtgtca gcagtggcg aggaggtgc 
ccatatggg tgcgaggcg agcagagat gggtctctg 
gcatcaccg gcgagacca cgtgccagg acgtcatcc 
aggtgctcc tccacgacg aggagagca ggggccgcc 
acatcggtt tgccagaac agtaccata taacagagc 
ggtggagag gcgtggagc agcctttag accgccagt 
ctgtcattt tagacattt ggaatgctt ggggaacag 
cacagcctg aagtcaaaa tatcactca gacgcatca 
aatatgaca ggccaccaa gtcgaagaa cccgctgcc 
ggtccgact cgagcactt ggtcagacc gcagcgctt 
ggaagacta tccacccga agaggctcc acgcgttac 
agaacgaaa aacaaccat caagtacgg aagaattac 
tgaaactag ttctagctc aaggagaag ttataagac 
gacaattaa agaagttga gacacagcg aacaccaaa 
tcggctatt tagaggata agacgcaca aggcccgcg 
tgcgcaaac acggcagca actacctcc tggagacgt 
acctaaagg gcttatccg aggctgtag accccgaaa 
atgacgcca atttgtgcg ctgacaaaa acagcgaca 
gtggagtca tgactgaag gcgacagcg agcagtcta 
ataccaagt cgagaccag cggaacaga cgtttgagc 
ggttttctc cctcgtccg aagagttca gcaaagaca 
taaaagacg acgacacga gctccacag tgagcgaag 
cgacgatca gggaacaaa tcgaattat tcgagtcta 
tagcgaaat tgaacaaac gtcttctca aggaagaag 
agagtttag ttaggctag atgcgaatc taaagaagt 
acgataagc acaaaacgg acgacttgg cgaaagcac 
tcactaatt tgaggacgg aaatgacta aaagtgcct 
gtgaaatgc aacataatg aggtggttc tcgaggaaa 
cgatggaga tgttgctta atcggcggc attacctgg 
agaatcttc acagggacc ttgctaatg aagagcagg 
agcacgaga tgctgcaag cgctgatgt tcagcacca 
ggaggcaga ggcacaacc cggacagtt gtagtccct 
actactcac ataccaaag aattgttag ataccttag 
tttagcgct tttaatccc taattaatt taataccaa 
atatcttgt acctaattc acgttggaa atttgttag 
tcattgatc tgaaatata tgtttttta caaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY