Status
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Gene information
General information
TC034179
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006764,TC006763,TC006762
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13840646..13847437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034179
    iBeetle screen iB_06223

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034179 RA
      MTCFVTLLL LSTLLVEGV KYTITKTGG ETIRQNAKK 
      ITSVRGAKK IVIEDEFEV FEKNVLEIT EKTPIFLMD 
      QNYSLRKIK SGVFLNQEI TETVVITNS QLKVVESGT 
      FVNLKIFEL VLKQNQITH LEKQAIVGL VNLEVINLA 
      NNHIVEFFD DSIVSSPAV FLDLAFNRL KKLGPNWFS 
      FMNSEKAVT ILLDNNRIG EIHILSFED VMLDTLNLR 
      RNKIGELPI GFFRGESIS QLFIFHSTM LAVLLLVFV 
      FLAVIYFKL VVPFFYWQK RDIFYVKPW TRFRKVFLG 
      KESFAEAVR AAYDEHPDK RYYGSYQFL QPSLFVRDL 
      DLIKAITVK DFEHFTDHS AFTSEKTDP ILGLNLFSL 
      KGQKWREMR STLSPAFTG SKMRAMFTL IAQTAESFT 
      QYLLGLGQG VVEVEMKDT CTKFTNDVI ANCAFGIEC 
      NSLLNPENE FYKMGTMVS KPSPWRLFK SLLYGFFPK 
      IFEFFRIPA LPPTISDFF LRIIKDAII VREQQGIVR 
      PDMIHLLLE ARKGRLKHD KIESNEPGF ATVQESTIG 
      QNEKSLQIT DELITAQAL VFFIAGFTT SSSLMSFLA 
      YELVVNPEI QKKMQKEID AFLDREFSY EQVLQMKYL 
      DQVVSEALR KYPPGYVLN RICVKDYVI KPKQEGEKV 
      AVIEKGCLV AIPVIALHY SPEFFPDPE KFNPERFSD 
      ENKGKILPG SYIPFGLGP RNCIGSRFA LLEIKVLLV 
      KLLAKFDLV PVDKTVIPL KFAKTLGLD AKGGIWVGL 
      KKRT
    CDS sequence
    • >TC034179 RA
      atgacgtgc ttcgtcacc ttactgttg ctttcaaca 
      ctgcttgtt gaaggagtg aaatacacg atcactaaa 
      actggaggt gagacaatt cgccaaaat gccaaaaaa 
      atcacaagt gtgagaggc gccaaaaaa atagtgatc 
      gaagacgag tttgaagtt tttgaaaaa aacgtcttg 
      gaaattacc gaaaaaact ccgattttt ctaatggac 
      cagaactac agtttgaga aaaatcaaa agtggggtt 
      ttccttaac caggaaatc accgaaact gttgtgata 
      accaacagc caattgaaa gtggtagag agtggcact 
      ttcgtgaac ttgaaaatt ttcgaactt gtgctcaag 
      caaaaccaa atcacgcat ttggaaaag caggcaatc 
      gtgggtttg gttaacttg gaagttatt aacttggca 
      aacaaccac attgttgag ttttttgac gattcgatc 
      gtctcctca ccggctgtg tttctggac ttggcgttc 
      aatcgcttg aaaaagttg ggtccgaat tggttcagt 
      ttcatgaac agtgaaaaa gcggtcact attctgttg 
      gataacaac agaattggg gaaatacac attttgagt 
      ttcgaggac gttatgttg gacacactt aacttgcgg 
      aggaacaaa ataggggaa ttaccgatt ggttttttt 
      cgcggtgaa agcatctct caactcttc attttccat 
      tcaaccatg ttagcagtg ttgctccta gtctttgtt 
      ttcctcgca gttatttat tttaaacta gttgtgccg 
      tttttctac tggcaaaag cgcgatatt ttttacgtt 
      aagccgtgg acacgtttc cggaaggtg tttttaggg 
      aaggaatcg tttgcggaa gctgtgcga gccgcttac 
      gacgaacat ccagataaa agatactat ggcagttat 
      cagttcctc caaccgtca cttttcgtc cgcgacttg 
      gacctaatc aaagccata acagtcaaa gactttgaa 
      cattttaca gaccactct gctttcacg agcgaaaaa 
      acggaccca attctcggt ctaaatctg ttctcttta 
      aagggccag aaatggcga gagatgcgg tcgacgcta 
      agtccggct tttaccgga agcaaaatg cgagcaatg 
      ttcacacta attgcgcaa actgccgaa agtttcact 
      caatatttg ctaggtctt ggtcaaggt gttgttgaa 
      gtggagatg aaagacact tgcacgaaa ttcacaaat 
      gatgtgatt gccaattgc gcctttgga attgaatgc 
      aactcgttg ttaaaccca gagaatgag ttttataaa 
      atgggcact atggtgtcc aaacctagc ccttggagg 
      ctgttcaaa agcctgctt tatgggttc tttccgaaa 
      atttttgag tttttccga attccagct ctgccccca 
      accatttcc gactttttc ttgcgaatt atcaaagac 
      gcgatcatt gtgagagaa cagcaagga atcgtaaga 
      cctgacatg atccacctc ctgctcgag gcccgcaaa 
      gggcgctta aagcacgac aaaatcgag tcaaacgaa 
      ccaggtttt gcaaccgtc caggaatca acaatcggc 
      caaaacgaa aagtcgctt caaatcact gacgaatta 
      atcacagcc caggccctt gttttcttc atcgccggt 
      ttcaccact tcgtccagc ttgatgagt tttctggcg 
      tacgaactt gtggtgaat cccgagatc cagaaaaaa 
      atgcaaaag gaaattgac gcatttctg gacagggaa 
      ttttcatac gaacaagtc ctccagatg aagtatttg 
      gaccaagtg gtgtctgaa gctctacgg aaatatccc 
      ccaggttac gtcctgaac cggatttgt gtcaaggac 
      tatgtcatt aaaccaaaa caggagggg gaaaaagtg 
      gcggttatt gaaaaaggt tgtttggtg gcgattccg 
      gtcattgcg ttacattac agtccggag tttttcccc 
      gatccggaa aaattcaac ccggagcgg ttcagtgac 
      gaaaataag ggaaaaatc ttgccaggg tcttacatc 
      ccctttggg ttagggcca cgaaattgc ataggatca 
      cgttttgct cttttggaa atcaaggtt ttgctagtc 
      aaacttttg gcaaagttt gacttggtt ccagtggac 
      aagacagtg attccgctc aagtttgcc aaaactttg 
      ggtttggac gcgaaaggg ggaatttgg gttgggttg 
      aagaaacga acataa
    mRNA sequence
    • >TC034179 RA
      gacaaacac aaaaattgt gattccacg atgacgtgc 
      ttcgtcacc ttactgttg ctttcaaca ctgcttgtt 
      gaaggagtg aaatacacg atcactaaa actggaggt 
      gagacaatt cgccaaaat gccaaaaaa atcacaagt 
      gtgagaggc gccaaaaaa atagtgatc gaagacgag 
      tttgaagtt tttgaaaaa aacgtcttg gaaattacc 
      gaaaaaact ccgattttt ctaatggac cagaactac 
      agtttgaga aaaatcaaa agtggggtt ttccttaac 
      caggaaatc accgaaact gttgtgata accaacagc 
      caattgaaa gtggtagag agtggcact ttcgtgaac 
      ttgaaaatt ttcgaactt gtgctcaag caaaaccaa 
      atcacgcat ttggaaaag caggcaatc gtgggtttg 
      gttaacttg gaagttatt aacttggca aacaaccac 
      attgttgag ttttttgac gattcgatc gtctcctca 
      ccggctgtg tttctggac ttggcgttc aatcgcttg 
      aaaaagttg ggtccgaat tggttcagt ttcatgaac 
      agtgaaaaa gcggtcact attctgttg gataacaac 
      agaattggg gaaatacac attttgagt ttcgaggac 
      gttatgttg gacacactt aacttgcgg aggaacaaa 
      ataggggaa ttaccgatt ggttttttt cgcggtgaa 
      agcatctct caactcttc attttccat tcaaccatg 
      ttagcagtg ttgctccta gtctttgtt ttcctcgca 
      gttatttat tttaaacta gttgtgccg tttttctac 
      tggcaaaag cgcgatatt ttttacgtt aagccgtgg 
      acacgtttc cggaaggtg tttttaggg aaggaatcg 
      tttgcggaa gctgtgcga gccgcttac gacgaacat 
      ccagataaa agatactat ggcagttat cagttcctc 
      caaccgtca cttttcgtc cgcgacttg gacctaatc 
      aaagccata acagtcaaa gactttgaa cattttaca 
      gaccactct gctttcacg agcgaaaaa acggaccca 
      attctcggt ctaaatctg ttctcttta aagggccag 
      aaatggcga gagatgcgg tcgacgcta agtccggct 
      tttaccgga agcaaaatg cgagcaatg ttcacacta 
      attgcgcaa actgccgaa agtttcact caatatttg 
      ctaggtctt ggtcaaggt gttgttgaa gtggagatg 
      aaagacact tgcacgaaa ttcacaaat gatgtgatt 
      gccaattgc gcctttgga attgaatgc aactcgttg 
      ttaaaccca gagaatgag ttttataaa atgggcact 
      atggtgtcc aaacctagc ccttggagg ctgttcaaa 
      agcctgctt tatgggttc tttccgaaa atttttgag 
      tttttccga attccagct ctgccccca accatttcc 
      gactttttc ttgcgaatt atcaaagac gcgatcatt 
      gtgagagaa cagcaagga atcgtaaga cctgacatg 
      atccacctc ctgctcgag gcccgcaaa gggcgctta 
      aagcacgac aaaatcgag tcaaacgaa ccaggtttt 
      gcaaccgtc caggaatca acaatcggc caaaacgaa 
      aagtcgctt caaatcact gacgaatta atcacagcc 
      caggccctt gttttcttc atcgccggt ttcaccact 
      tcgtccagc ttgatgagt tttctggcg tacgaactt 
      gtggtgaat cccgagatc cagaaaaaa atgcaaaag 
      gaaattgac gcatttctg gacagggaa ttttcatac 
      gaacaagtc ctccagatg aagtatttg gaccaagtg 
      gtgtctgaa gctctacgg aaatatccc ccaggttac 
      gtcctgaac cggatttgt gtcaaggac tatgtcatt 
      aaaccaaaa caggagggg gaaaaagtg gcggttatt 
      gaaaaaggt tgtttggtg gcgattccg gtcattgcg 
      ttacattac agtccggag tttttcccc gatccggaa 
      aaattcaac ccggagcgg ttcagtgac gaaaataag 
      ggaaaaatc ttgccaggg tcttacatc ccctttggg 
      ttagggcca cgaaattgc ataggatca cgttttgct 
      cttttggaa atcaaggtt ttgctagtc aaacttttg 
      gcaaagttt gacttggtt ccagtggac aagacagtg 
      attccgctc aagtttgcc aaaactttg ggtttggac 
      gcgaaaggg ggaatttgg gttgggttg aagaaacga 
      acataattg tagttgtat tttttattt taataaata 
      gcttagact ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY