Status
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Gene information
General information
TC034179
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006764,TC006763,TC006762 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13840646..13847437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034179
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06223

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034179 RA
      MTCFVTLLL LSTLLVEGV KYTITKTGG ETIRQNAKK 
ITSVRGAKK IVIEDEFEV FEKNVLEIT EKTPIFLMD 
QNYSLRKIK SGVFLNQEI TETVVITNS QLKVVESGT 
FVNLKIFEL VLKQNQITH LEKQAIVGL VNLEVINLA 
NNHIVEFFD DSIVSSPAV FLDLAFNRL KKLGPNWFS 
FMNSEKAVT ILLDNNRIG EIHILSFED VMLDTLNLR 
RNKIGELPI GFFRGESIS QLFIFHSTM LAVLLLVFV 
FLAVIYFKL VVPFFYWQK RDIFYVKPW TRFRKVFLG 
KESFAEAVR AAYDEHPDK RYYGSYQFL QPSLFVRDL 
DLIKAITVK DFEHFTDHS AFTSEKTDP ILGLNLFSL 
KGQKWREMR STLSPAFTG SKMRAMFTL IAQTAESFT 
QYLLGLGQG VVEVEMKDT CTKFTNDVI ANCAFGIEC 
NSLLNPENE FYKMGTMVS KPSPWRLFK SLLYGFFPK 
IFEFFRIPA LPPTISDFF LRIIKDAII VREQQGIVR 
PDMIHLLLE ARKGRLKHD KIESNEPGF ATVQESTIG 
QNEKSLQIT DELITAQAL VFFIAGFTT SSSLMSFLA 
YELVVNPEI QKKMQKEID AFLDREFSY EQVLQMKYL 
DQVVSEALR KYPPGYVLN RICVKDYVI KPKQEGEKV 
AVIEKGCLV AIPVIALHY SPEFFPDPE KFNPERFSD 
ENKGKILPG SYIPFGLGP RNCIGSRFA LLEIKVLLV 
KLLAKFDLV PVDKTVIPL KFAKTLGLD AKGGIWVGL 
KKRT
    CDS sequence
    • >TC034179 RA
      atgacgtgc ttcgtcacc ttactgttg ctttcaaca 
ctgcttgtt gaaggagtg aaatacacg atcactaaa 
actggaggt gagacaatt cgccaaaat gccaaaaaa 
atcacaagt gtgagaggc gccaaaaaa atagtgatc 
gaagacgag tttgaagtt tttgaaaaa aacgtcttg 
gaaattacc gaaaaaact ccgattttt ctaatggac 
cagaactac agtttgaga aaaatcaaa agtggggtt 
ttccttaac caggaaatc accgaaact gttgtgata 
accaacagc caattgaaa gtggtagag agtggcact 
ttcgtgaac ttgaaaatt ttcgaactt gtgctcaag 
caaaaccaa atcacgcat ttggaaaag caggcaatc 
gtgggtttg gttaacttg gaagttatt aacttggca 
aacaaccac attgttgag ttttttgac gattcgatc 
gtctcctca ccggctgtg tttctggac ttggcgttc 
aatcgcttg aaaaagttg ggtccgaat tggttcagt 
ttcatgaac agtgaaaaa gcggtcact attctgttg 
gataacaac agaattggg gaaatacac attttgagt 
ttcgaggac gttatgttg gacacactt aacttgcgg 
aggaacaaa ataggggaa ttaccgatt ggttttttt 
cgcggtgaa agcatctct caactcttc attttccat 
tcaaccatg ttagcagtg ttgctccta gtctttgtt 
ttcctcgca gttatttat tttaaacta gttgtgccg 
tttttctac tggcaaaag cgcgatatt ttttacgtt 
aagccgtgg acacgtttc cggaaggtg tttttaggg 
aaggaatcg tttgcggaa gctgtgcga gccgcttac 
gacgaacat ccagataaa agatactat ggcagttat 
cagttcctc caaccgtca cttttcgtc cgcgacttg 
gacctaatc aaagccata acagtcaaa gactttgaa 
cattttaca gaccactct gctttcacg agcgaaaaa 
acggaccca attctcggt ctaaatctg ttctcttta 
aagggccag aaatggcga gagatgcgg tcgacgcta 
agtccggct tttaccgga agcaaaatg cgagcaatg 
ttcacacta attgcgcaa actgccgaa agtttcact 
caatatttg ctaggtctt ggtcaaggt gttgttgaa 
gtggagatg aaagacact tgcacgaaa ttcacaaat 
gatgtgatt gccaattgc gcctttgga attgaatgc 
aactcgttg ttaaaccca gagaatgag ttttataaa 
atgggcact atggtgtcc aaacctagc ccttggagg 
ctgttcaaa agcctgctt tatgggttc tttccgaaa 
atttttgag tttttccga attccagct ctgccccca 
accatttcc gactttttc ttgcgaatt atcaaagac 
gcgatcatt gtgagagaa cagcaagga atcgtaaga 
cctgacatg atccacctc ctgctcgag gcccgcaaa 
gggcgctta aagcacgac aaaatcgag tcaaacgaa 
ccaggtttt gcaaccgtc caggaatca acaatcggc 
caaaacgaa aagtcgctt caaatcact gacgaatta 
atcacagcc caggccctt gttttcttc atcgccggt 
ttcaccact tcgtccagc ttgatgagt tttctggcg 
tacgaactt gtggtgaat cccgagatc cagaaaaaa 
atgcaaaag gaaattgac gcatttctg gacagggaa 
ttttcatac gaacaagtc ctccagatg aagtatttg 
gaccaagtg gtgtctgaa gctctacgg aaatatccc 
ccaggttac gtcctgaac cggatttgt gtcaaggac 
tatgtcatt aaaccaaaa caggagggg gaaaaagtg 
gcggttatt gaaaaaggt tgtttggtg gcgattccg 
gtcattgcg ttacattac agtccggag tttttcccc 
gatccggaa aaattcaac ccggagcgg ttcagtgac 
gaaaataag ggaaaaatc ttgccaggg tcttacatc 
ccctttggg ttagggcca cgaaattgc ataggatca 
cgttttgct cttttggaa atcaaggtt ttgctagtc 
aaacttttg gcaaagttt gacttggtt ccagtggac 
aagacagtg attccgctc aagtttgcc aaaactttg 
ggtttggac gcgaaaggg ggaatttgg gttgggttg 
aagaaacga acataa
    mRNA sequence
    • >TC034179 RA
      gacaaacac aaaaattgt gattccacg atgacgtgc 
ttcgtcacc ttactgttg ctttcaaca ctgcttgtt 
gaaggagtg aaatacacg atcactaaa actggaggt 
gagacaatt cgccaaaat gccaaaaaa atcacaagt 
gtgagaggc gccaaaaaa atagtgatc gaagacgag 
tttgaagtt tttgaaaaa aacgtcttg gaaattacc 
gaaaaaact ccgattttt ctaatggac cagaactac 
agtttgaga aaaatcaaa agtggggtt ttccttaac 
caggaaatc accgaaact gttgtgata accaacagc 
caattgaaa gtggtagag agtggcact ttcgtgaac 
ttgaaaatt ttcgaactt gtgctcaag caaaaccaa 
atcacgcat ttggaaaag caggcaatc gtgggtttg 
gttaacttg gaagttatt aacttggca aacaaccac 
attgttgag ttttttgac gattcgatc gtctcctca 
ccggctgtg tttctggac ttggcgttc aatcgcttg 
aaaaagttg ggtccgaat tggttcagt ttcatgaac 
agtgaaaaa gcggtcact attctgttg gataacaac 
agaattggg gaaatacac attttgagt ttcgaggac 
gttatgttg gacacactt aacttgcgg aggaacaaa 
ataggggaa ttaccgatt ggttttttt cgcggtgaa 
agcatctct caactcttc attttccat tcaaccatg 
ttagcagtg ttgctccta gtctttgtt ttcctcgca 
gttatttat tttaaacta gttgtgccg tttttctac 
tggcaaaag cgcgatatt ttttacgtt aagccgtgg 
acacgtttc cggaaggtg tttttaggg aaggaatcg 
tttgcggaa gctgtgcga gccgcttac gacgaacat 
ccagataaa agatactat ggcagttat cagttcctc 
caaccgtca cttttcgtc cgcgacttg gacctaatc 
aaagccata acagtcaaa gactttgaa cattttaca 
gaccactct gctttcacg agcgaaaaa acggaccca 
attctcggt ctaaatctg ttctcttta aagggccag 
aaatggcga gagatgcgg tcgacgcta agtccggct 
tttaccgga agcaaaatg cgagcaatg ttcacacta 
attgcgcaa actgccgaa agtttcact caatatttg 
ctaggtctt ggtcaaggt gttgttgaa gtggagatg 
aaagacact tgcacgaaa ttcacaaat gatgtgatt 
gccaattgc gcctttgga attgaatgc aactcgttg 
ttaaaccca gagaatgag ttttataaa atgggcact 
atggtgtcc aaacctagc ccttggagg ctgttcaaa 
agcctgctt tatgggttc tttccgaaa atttttgag 
tttttccga attccagct ctgccccca accatttcc 
gactttttc ttgcgaatt atcaaagac gcgatcatt 
gtgagagaa cagcaagga atcgtaaga cctgacatg 
atccacctc ctgctcgag gcccgcaaa gggcgctta 
aagcacgac aaaatcgag tcaaacgaa ccaggtttt 
gcaaccgtc caggaatca acaatcggc caaaacgaa 
aagtcgctt caaatcact gacgaatta atcacagcc 
caggccctt gttttcttc atcgccggt ttcaccact 
tcgtccagc ttgatgagt tttctggcg tacgaactt 
gtggtgaat cccgagatc cagaaaaaa atgcaaaag 
gaaattgac gcatttctg gacagggaa ttttcatac 
gaacaagtc ctccagatg aagtatttg gaccaagtg 
gtgtctgaa gctctacgg aaatatccc ccaggttac 
gtcctgaac cggatttgt gtcaaggac tatgtcatt 
aaaccaaaa caggagggg gaaaaagtg gcggttatt 
gaaaaaggt tgtttggtg gcgattccg gtcattgcg 
ttacattac agtccggag tttttcccc gatccggaa 
aaattcaac ccggagcgg ttcagtgac gaaaataag 
ggaaaaatc ttgccaggg tcttacatc ccctttggg 
ttagggcca cgaaattgc ataggatca cgttttgct 
cttttggaa atcaaggtt ttgctagtc aaacttttg 
gcaaagttt gacttggtt ccagtggac aagacagtg 
attccgctc aagtttgcc aaaactttg ggtttggac 
gcgaaaggg ggaatttgg gttgggttg aagaaacga 
acataattg tagttgtat tttttattt taataaata 
gcttagact ttt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY