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Gene information
General information
TC006709 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13174836..13177070
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006709
    iBeetle screen iB_06221

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006709 RA
      MAVLLENVT NCVWQAFDA LQQDKSGYV HKSKLKVLT 
      ANIGTILDL YGVERGLEH FRSTPTLNF DQYRFYLQK 
      EVFSSLPNK LPLAELRDY EARIGEVCW LVCRKKFVQ 
      RDNKVLADE SLFQIFRIF CLLGDLIRD QHNEDTYQV 
      LLHPSEVYN IAQTLANSL GCPWDEEDF TNLSISMGS 
      FRLTPFIAV LESRSLVGV KDHFAISEA VTDIYQTLV 
      EDVIKKGYL TKKGYIFPT MREYWFVLR PSELSYYKT 
      RSEKDRCGS LPIEPGSRV EAKSGYRII LHTPERSFE 
      LGTTDHMTR VQWISALQL AADHSGGCQ SYQRLQVAR 
      RKMQRAGRI QEMMRARAQ LQQERSARQ AAEGQAKEL 
      EAVVKEEAR KLTDLEQMR AKLERLLEE ETQAKRDEE 
      IVRALQARV LAEEWEKRE ELERLQEEQ RTLLEEERG 
      KRKEFEELQ REKEQQLRE AESRLVQLE KEREALDVQ 
      LKLAHDKIK LSEDRRELM EARLYQVVP LREGDRVRR 
      AHSFMPSTK ERPVLLEVR ASTLRRSKN
    CDS sequence
    • >TC006709 RA
      atggccgtg ctcctcgaa aacgtgaca aattgcgtg 
      tggcaagcc tttgatgcc ctacaacag gacaagtcc 
      ggatacgtc cacaagtcc aaacttaag gttctgacc 
      gcgaatatt ggcacgatc ttggatttg tacggggtt 
      gaacggggg ctggagcat ttccggagc acccccacc 
      ttaaacttc gaccagtac aggttttac ttgcaaaag 
      gaagtgttc tcgtcctta cccaacaaa ctcccattg 
      gcggaactg cgcgactat gaggcccgc attggcgaa 
      gtttgctgg ctggtgtgt cggaaaaaa ttcgttcag 
      agagacaat aaagttttg gcggacgaa tcccttttc 
      caaattttc cggattttc tgtcttttg ggcgacttg 
      atccgcgac caacacaac gaggacact taccaagtc 
      ctcctgcac ccttccgaa gtctacaac atcgcccaa 
      actttggcc aactccctg ggatgtccc tgggacgag 
      gaggatttc accaacttg tccatctcc atggggagt 
      ttccgccta actcccttc atcgccgtt ctcgaatct 
      cgatccttg gtcggagtc aaggaccat ttcgcaatt 
      tcggaagct gtaaccgac atttaccag accctcgtc 
      gaagacgtc atcaagaaa ggttacttg accaaaaaa 
      ggctacatt ttccccaca atgcgcgaa tattggttc 
      gtgctccgg ccttcagaa ctaagttat tacaaaaca 
      cgttcggag aaggaccgt tgcggctca ctccccatc 
      gagcccgga agccgagtc gaggccaaa tccgggtac 
      cggatcatc ctccacacc ccggaacgg agcttcgag 
      ttgggaacc accgaccac atgactcga gtccagtgg 
      atctcggct ctccaactc gcagctgac cattccggg 
      ggctgccag agctaccaa agactgcaa gtcgctcgg 
      cgaaaaatg caaagggct ggaaggatc caggaaatg 
      atgagggct agagcacaa ctgcagcag gaacgcagt 
      gcgcggcaa gcagccgaa gggcaggca aaggagttg 
      gaggccgtt gtgaaggaa gaagcgcgg aaattgacc 
      gacttggag caaatgaga gcaaagttg gaacgacta 
      cttgaggag gaaacgcaa gcgaagcgt gacgaagaa 
      attgtgcga gccctccaa gctagagtt ttagccgaa 
      gagtgggag aaacgggag gagttggag cggctccag 
      gaggagcaa cgaactctt ctggaggag gaacgtggc 
      aaaaggaag gaatttgag gagttgcaa cgtgaaaaa 
      gaacaacaa ttgcgagag gcggaatcg cgcttggtt 
      cagttggaa aaagaacgg gaggcgcta gacgtccag 
      ttaaaactg gcccacgac aaaattaaa ctctcagaa 
      gaccgtaga gaactaatg gaagcgagg ttgtaccaa 
      gtggtaccg ctccgggaa ggtgaccgg gtcagacga 
      gctcattcc ttcatgccc agcacgaaa gaacgccca 
      gttttgtta gaagtgaga gcttccact ctaagacgc 
      tccaaaaat tga
    mRNA sequence
    • >TC006709 RA
      atattgtta ttagtttgt ttataacat aagtggtgg 
      tactttaaa ttctttaca atttaatag ttttttgac 
      ttcgttcac cacacttgt gtagtgact caagtgcct 
      cccatatgc acagaaagc atggccgtg ctcctcgaa 
      aacgtgaca aattgcgtg tggcaagcc tttgatgcc 
      ctacaacag gacaagtcc ggatacgtc cacaagtcc 
      aaacttaag gttctgacc gcgaatatt ggcacgatc 
      ttggatttg tacggggtt gaacggggg ctggagcat 
      ttccggagc acccccacc ttaaacttc gaccagtac 
      aggttttac ttgcaaaag gaagtgttc tcgtcctta 
      cccaacaaa ctcccattg gcggaactg cgcgactat 
      gaggcccgc attggcgaa gtttgctgg ctggtgtgt 
      cggaaaaaa ttcgttcag agagacaat aaagttttg 
      gcggacgaa tcccttttc caaattttc cggattttc 
      tgtcttttg ggcgacttg atccgcgac caacacaac 
      gaggacact taccaagtc ctcctgcac ccttccgaa 
      gtctacaac atcgcccaa actttggcc aactccctg 
      ggatgtccc tgggacgag gaggatttc accaacttg 
      tccatctcc atggggagt ttccgccta actcccttc 
      atcgccgtt ctcgaatct cgatccttg gtcggagtc 
      aaggaccat ttcgcaatt tcggaagct gtaaccgac 
      atttaccag accctcgtc gaagacgtc atcaagaaa 
      ggttacttg accaaaaaa ggctacatt ttccccaca 
      atgcgcgaa tattggttc gtgctccgg ccttcagaa 
      ctaagttat tacaaaaca cgttcggag aaggaccgt 
      tgcggctca ctccccatc gagcccgga agccgagtc 
      gaggccaaa tccgggtac cggatcatc ctccacacc 
      ccggaacgg agcttcgag ttgggaacc accgaccac 
      atgactcga gtccagtgg atctcggct ctccaactc 
      gcagctgac cattccggg ggctgccag agctaccaa 
      agactgcaa gtcgctcgg cgaaaaatg caaagggct 
      ggaaggatc caggaaatg atgagggct agagcacaa 
      ctgcagcag gaacgcagt gcgcggcaa gcagccgaa 
      gggcaggca aaggagttg gaggccgtt gtgaaggaa 
      gaagcgcgg aaattgacc gacttggag caaatgaga 
      gcaaagttg gaacgacta cttgaggag gaaacgcaa 
      gcgaagcgt gacgaagaa attgtgcga gccctccaa 
      gctagagtt ttagccgaa gagtgggag aaacgggag 
      gagttggag cggctccag gaggagcaa cgaactctt 
      ctggaggag gaacgtggc aaaaggaag gaatttgag 
      gagttgcaa cgtgaaaaa gaacaacaa ttgcgagag 
      gcggaatcg cgcttggtt cagttggaa aaagaacgg 
      gaggcgcta gacgtccag ttaaaactg gcccacgac 
      aaaattaaa ctctcagaa gaccgtaga gaactaatg 
      gaagcgagg ttgtaccaa gtggtaccg ctccgggaa 
      ggtgaccgg gtcagacga gctcattcc ttcatgccc 
      agcacgaaa gaacgccca gttttgtta gaagtgaga 
      gcttccact ctaagacgc tccaaaaat tgaacaatg 
      ctcattcca ttttttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY