| General information | |||
| TC006425 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9015944..9022878 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006425 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06215 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
- >TC006425 RA
MASNSDGSG SNGTSPRNL SINASRDYD TLKQQYEKA NSELSNSRR RCDHALNDL DYFREQHRA AMNQLEVSS QDSASLRTR YSELLSEKQ RLEQENQRL RKELNEVHR QQQDVVLLA ENGNSDSLY ISHSQALSK LELAKDENN RLAKQFEQE RNNAQREID SLKQQVLKY YKAHQKVQQ QYEEAEKQI VAIKIKANK EVKRLTDER NATLAEYTL VMSERDQVH KEMEKLSDD LAQALKKGK MLDADNQEL VGEKQAIGY QLESLRREI ASALYERDK ALKECADLR EKFGELGAS NDDNRRREQ TRSRHLEGL GGPCENVGR KDALQSDVR TIGQRQRLD NLDQANQEL ESLRKSLDK AQTELSEAI QEAEVSKGR RDWAFSERD KIVQERESI RTLCDNMRK ERDRAVSEL AESLRESDA LKKQRNELL KEVKALKEM LEAHIVDHD DDHFSDIDD STEILEVEL TCDGASDLG LELAGGRDD PHFPGDGAV YVAAVAEGG IADGKLRPN DRISRVNDV DCSAVSRRM VIEAIRASL PVARIVVSR RRRRGPSPS PSIASQVTK WVHTARLVP GCHGLTLNT GVYISRISD GSLAAKDKS LSVGDRILR INEKSMDDI ESIHEAMRV LNDDSVDVI TVTALKISH PECPNVFTP VRRHKRENR SSQTDEHDY LSNQQEKNN GGAWFKEKI DLMRGRRHA KDNDKKKYR NSSPSPLEL ADPEQAIAE LDLVLDSYR GNANVGTVK RTKRGTKEQ EKNGGTWPK AKATALLQP VLGSTVVVR NKERPPLSL LKEAGAEYH RNSTPVPLP IASVGPRAT NRHSVYKSV DGGGSHFST TGDSFEKLR LAQSNRLSA NMNSDNSLD YPVHRYADK DIHTYYKKN KLGKYAFDC DRDSVVGHT ESSQGNSSV PTSHSRIHS QFWVPPRTY YPFHPHPHV DFEHSIHHT HSPSVDGAS RKAINPAGP MPYRETDLL EGGTFPRKN QRFVVPSNP SITSKVSTG SIERSSERG SPMPIFHVE VLSPGEAGS SGKECCSWV GGQYKPLPG ELRRVHIDK SNEPLGIQI NCRESGGIF VSTVNDNSL ASRVGLQIG DQLLEVCGI NMRNATYNL AANVLRQCG NSITMLVQY SPDKYNELE GSGSCSGSS SNDDEGDGE TTPCNSPKE IRKSHPVGI TKLPPPTSL LRVGQQTSS LQRQNNSSR DDDEPRFLY IETLKTSNL GISLVGGNA AGIFIHSVQ PDSLAYHAG LRTGDQILE YNGSDLRNA TAEEAAYEL AKPADKVTV SAHYRIDRY NEIKDKPGD SLYVRCGFD RSGNEHTEP PQLAFAKDD VLYVDNTMF NGVPGQWRA WRLDNEGRR QQCGVIPSK YKVEEEMLL RRGADLDQT GRPVSVGSS IARRSFFRR KKHQRHSKE LASFCSLAG PGWYSDGGS LHEEPAPPP AYQRVERLP FPEFRPVLV LGPLAECVV DKLVSDFPD EFGRAPAET RRCSQAQLD RELADGHLV EYRRRGSCW ECVTVAAVR QVSRAHLHC MLDVAASSV ERLHRHGIY PVVLLIKFK STKQIREVK DALRYSLDK LSGKAAKEM FEHGHKLEA EYRHLVTAV VAAGANVAH VCAQVKAAV DAEHRKSQW VPVG
- >TC006425 RA
atggcgtca aatagcgat ggtagtgga agcaatggt acttcgccg agaaactta agcataaat gcttcaaga gactatgac acactcaaa caacaatac gaaaaagct aacagtgaa ttaagcaac tcgagaaga cgttgcgat catgctctc aatgatctg gactacttt agggaacaa cacagagcg gcaatgaac cagctagaa gtctcgtct caagactcg gcctctctc agaacaaga tactcagaa ttgttaagc gaaaaacag agattagaa caagaaaat cagagatta agaaaagag ttaaatgag gtgcaccga caacagcag gatgttgtt ttgcttgca gagaatgga aattcagat tctttatac atttcacat tcacaggct ttatcaaag ttggaactg gcaaaagat gagaataac cggttagcg aagcagttt gaacaggaa agaaacaac gcacaacgt gagatcgac agtttgaag caacaggtg ctcaaatac tacaaagcc caccagaaa gtccaacaa caatatgag gaagccgag aagcagatc gttgcaata aaaatcaaa gcaaacaaa gaagtgaaa cgcttaacc gacgaacgg aacgcaact ctagccgaa tacacttta gtcatgagc gagagagac caagtccac aaagaaatg gaaaaattg agcgacgac ttggcccaa gccctgaaa aagggcaaa atgttggac gccgacaac caagaacta gtcggcgag aaacaagca attgggtac cagttagaa tcgctccgg cgcgaaatc gcaagcgcc ctctacgaa cgcgataaa gccttaaag gagtgcgcc gaccttcgc gaaaagttc ggcgagttg ggggcctcc aacgacgat aatcgccgg agggagcag acgaggtcg cggcacttg gaagggttg ggaggtcct tgcgaaaat gtgggtcgg aaggatgcc ctccaatcg gacgtgcgg acgattggc caaaggcaa aggcttgat aatttggac caagccaat caagagttg gaatcgcta cgcaagagt ttagataag gcgcagact gagttatcg gaggccatc caagaggcg gaagtgtcg aaagggaga agggactgg gctttttcc gagcgtgat aaaatcgtc caagagcgc gaaagcata aggactttg tgtgataac atgcggaag gaacgcgat cgggccgtt tcggaactt gccgaatcg ctccgggag tcggacgcg ttgaagaag caacggaac gagctcctt aaggaagtc aaggcttta aaggagatg cttgaggcg catattgtt gatcatgat gacgatcat ttcagcgat attgacgac agtaccgag atattggag gttgagttg acttgtgac ggggcgtct gatttggga ttagagttg gctgggggt cgtgatgat ccccatttt ccgggagac ggcgctgtg tatgtcgct gctgtggcg gaaggcgga atagccgac gggaaattg cgaccaaac gatcgcatt tccagagtc aacgatgtc gattgtagt gctgtctca cggcggatg gtcattgaa gctattagg gctagtctc cctgtggct agaatcgtg gttagtcgg agacgccga cgggggcct agcccttca cctagtatc gcttcacag gtgaccaaa tgggttcat accgctcgt ttggttcct ggctgccac ggtcttacc ctaaacacc ggggtttac attagtaga attagcgac gggagtctc gccgctaaa gacaagagt ctctcagtc ggggaccga atactccga atcaacgaa aaatcaatg gatgatata gaaagcatc catgaagcc atgcgagtc cttaacgac gactccgtc gacgttatc accgtcaca gcgctgaag ataagccac cccgagtgt cccaacgtt ttcactcct gtccgccgt cacaagcgc gaaaatcgt agctcgcag acggacgaa cacgattat ctctccaac cagcaagag aaaaacaac ggcggggcg tggtttaaa gagaagatt gatctgatg agaggtaga cgtcatgcc aaagataac gataagaaa aagtatcgg aacagttcg cccagtcct ttggaattg gccgatccg gagcaagcc atcgccgaa ctggacttg gttttggac agttataga gggaatgcc aacgtcgga actgtgaaa cgaacgaaa cgagggacg aaggaacag gagaagaat ggaggaacc tggcccaag gcgaaagcc acggcgttg cttcagcca gtccttgga agtaccgtg gtggtacgc aacaaggaa cggcctcct ctgagtttg ctgaaggag gcaggcgcc gaatatcac agaaactcc actcctgtg ccactgccc atagcttcg gtgggtccg cgggcgacc aaccgccac tccgtctac aaaagcgta gacggcggt ggttcccac tttagcacc accggggat tccttcgaa aaacttcgt ttagcccaa tcaaaccgc ctaagtgcc aacatgaac tcggacaac agtctcgac taccccgtc catcgctac gccgataag gacatccac acctactac aagaaaaac aagctcggg aaatacgcc tttgactgt gatcgcgac agtgtcgtc ggccacact gagtcttcc caagggaac agctccgtc cccacttcc cacagccgc atccacagc cagttctgg gtgccccct cgaacttac tacccgttc catccacac cctcacgtc gatttcgag cattcgatc caccacaca cacagtcct tcggtagac ggcgccagt cgcaaggca atcaacccc gccggtccg atgccatac cgagagacg gatcttctg gaaggaggg acgtttccg cggaaaaac caacgattc gtagtgccc tcgaacccg agcatcact tcgaaagtc agtaccggg agtatagaa cgcagctcg gaacggggc agtcccatg cccattttt cacgtcgaa gttttgagc ccgggcgag gccgggagt agcggcaaa gagtgctgt tcgtgggtc ggaggccag tataagccg ctcccgggg gaacttcga cgcgtgcac atcgacaaa tcgaacgaa ccgttagga attcaaatc aattgtcgc gaaagtggc gggattttt gtgtcaact gtgaatgat aatagttta gcgagtcgc gtggggttg caaatcggg gaccagctg ctggaggtg tgtgggatt aatatgagg aacgccacg tataacctg gcggcgaat gtgttgcga cagtgcggg aattccatc actatgctg gtgcaatac agccctgat aaatacaac gaattggaa gggtcaggt tcttgttcc ggctctagt tcaaacgac gacgaaggt gatggcgaa accacaccg tgtaactcg cccaaggaa atccgaaaa tctcatcca gttggcata actaagtta ccaccgccc acttcgtta ttaagggtt gggcagcag acctcctca ctacaaagg caaaataac agctccagg gacgatgat gagccccgt tttctctac atcgagacg ctgaaaacg tccaatttg gggatttcc ctagtcggg ggcaacgcc gctggcatt tttattcat tcggtccag cccgattct ctagcatac catgccggt ttacgcacc ggtgaccaa atcctggaa tacaacggg agcgacctc cgcaatgcc acggccgaa gaagcggcc tacgaattg gccaaaccg gccgataaa gtcaccgtt tcggcccac taccgcatc gatagatac aacgaaatc aaagacaaa ccgggcgat agtttgtac gtccgttgc ggtttcgac cgctcggga aacgaacac acggaacca cctcaactc gcttttgcc aaagacgat gtcctctat gtcgacaat acgatgttt aacggagtt ccaggtcaa tggcgtgct tggaggttg gataatgag ggtcgccgc cagcagtgc ggagtgatc cctagtaaa tataaggtt gaggaggag atgctccta cggagaggt gccgatttg gaccaaacc ggacgcccg gtttcggtc ggatcgtcc atagcccgt cggagtttc ttccggcgg aaaaagcac caacgccat tcgaaagaa ctggccagt ttttgcagc ctcgcggga cctgggtgg tactccgac ggcgggtct ttgcacgag gagccggcg cctcccccg gcttaccag cgcgtggag aggctgcct ttccccgag tttcggccg gttttggtg ctgggccct ttggccgaa tgtgtggtg gataaatta gtgagtgat ttcccggat gagttcggg cgggcacct gcagagacc cggcgctgc tcccaggcg cagcttgac cgggagttg gccgatggc cacctggtg gagtaccgc cgacggggg agctgctgg gagtgcgtc actgtggcc gcagtgagg caagtctcc agagctcac ctccattgt atgttggac gtggcggca agcagtgtc gagcggctc catcggcat ggtatatac ccggtggta ctcctcatc aagttcaag agtaccaag caaatccgg gaggtgaag gacgccctg aggtacagc ttggataag ttgtcgggc aaagcagcg aaggagatg ttcgagcat gggcataaa cttgaagct gagtaccgg catttggtg accgctgtt gtagcagct ggagcgaat gtggcacat gtttgtgct caggttaaa gccgccgtt gatgcggaa cataggaag agtcagtgg gttccggtc ggctga
- >TC006425 RA
tggtaaaag acgaagtag cagtaaaga tggtggtta gtagttaaa ttttaaatt aaattagga ataaccaaa tgtgatgat tcgtgaatt cacggcatc ctgagggtg tgatggcgt caaatagcg atggtagtg gaagcaatg gtacttcgc cgagaaact taagcataa atgcttcaa gagactatg acacactca aacaacaat acgaaaaag ctaacagtg aattaagca actcgagaa gacgttgcg atcatgctc tcaatgatc tggactact ttagggaac aacacagag cggcaatga accagctag aagtctcgt ctcaagact cggcctctc tcagaacaa gatactcag aattgttaa gcgaaaaac agagattag aacaagaaa atcagagat taagaaaag agttaaatg aggtgcacc gacaacagc aggatgttg ttttgcttg cagagaatg gaaattcag attctttat acatttcac attcacagg ctttatcaa agttggaac tggcaaaag atgagaata accggttag cgaagcagt ttgaacagg aaagaaaca acgcacaac gtgagatcg acagtttga agcaacagg tgctcaaat actacaaag cccaccaga aagtccaac aacaatatg aggaagccg agaagcaga tcgttgcaa taaaaatca aagcaaaca aagaagtga aacgcttaa ccgacgaac ggaacgcaa ctctagccg aatacactt tagtcatga gcgagagag accaagtcc acaaagaaa tggaaaaat tgagcgacg acttggccc aagccctga aaaagggca aaatgttgg acgccgaca accaagaac tagtcggcg agaaacaag caattgggt accagttag aatcgctcc ggcgcgaaa tcgcaagcg ccctctacg aacgcgata aagccttaa aggagtgcg ccgaccttc gcgaaaagt tcggcgagt tgggggcct ccaacgacg ataatcgcc ggagggagc agacgaggt cgcggcact tggaagggt tgggaggtc cttgcgaaa atgtgggtc ggaaggatg ccctccaat cggacgtgc ggacgattg gccaaaggc aaaggcttg ataatttgg accaagcca atcaagagt tggaatcgc tacgcaaga gtttagata aggcgcaga ctgagttat cggaggcca tccaagagg cggaagtgt cgaaaggga gaagggact gggcttttt ccgagcgtg ataaaatcg tccaagagc gcgaaagca taaggactt tgtgtgata acatgcgga aggaacgcg atcgggccg tttcggaac ttgccgaat cgctccggg agtcggacg cgttgaaga agcaacgga acgagctcc ttaaggaag tcaaggctt taaaggaga tgcttgagg cgcatattg ttgatcatg atgacgatc atttcagcg atattgacg acagtaccg agatattgg aggttgagt tgacttgtg acggggcgt ctgatttgg gattagagt tggctgggg gtcgtgatg atccccatt ttccgggag acggcgctg tgtatgtcg ctgctgtgg cggaaggcg gaatagccg acgggaaat tgcgaccaa acgatcgca tttccagag tcaacgatg tcgattgta gtgctgtct cacggcgga tggtcattg aagctatta gggctagtc tccctgtgg ctagaatcg tggttagtc ggagacgcc gacgggggc ctagccctt cacctagta tcgcttcac aggtgacca aatgggttc ataccgctc gtttggttc ctggctgcc acggtctta ccctaaaca ccggggttt acattagta gaattagcg acgggagtc tcgccgcta aagacaaga gtctctcag tcggggacc gaatactcc gaatcaacg aaaaatcaa tggatgata tagaaagca tccatgaag ccatgcgag tccttaacg acgactccg tcgacgtta tcaccgtca cagcgctga agataagcc accccgagt gtcccaacg ttttcactc ctgtccgcc gtcacaagc gcgaaaatc gtagctcgc agacggacg aacacgatt atctctcca accagcaag agaaaaaca acggcgggg cgtggttta aagagaaga ttgatctga tgagaggta gacgtcatg ccaaagata acgataaga aaaagtatc ggaacagtt cgcccagtc ctttggaat tggccgatc cggagcaag ccatcgccg aactggact tggttttgg acagttata gagggaatg ccaacgtcg gaactgtga aacgaacga aacgaggga cgaaggaac aggagaaga atggaggaa cctggccca aggcgaaag ccacggcgt tgcttcagc cagtccttg gaagtaccg tggtggtac gcaacaagg aacggcctc ctctgagtt tgctgaagg aggcaggcg ccgaatatc acagaaact ccactcctg tgccactgc ccatagctt cggtgggtc cgcgggcga ccaaccgcc actccgtct acaaaagcg tagacggcg gtggttccc actttagca ccaccgggg attccttcg aaaaacttc gtttagccc aatcaaacc gcctaagtg ccaacatga actcggaca acagtctcg actaccccg tccatcgct acgccgata aggacatcc acacctact acaagaaaa acaagctcg ggaaatacg cctttgact gtgatcgcg acagtgtcg tcggccaca ctgagtctt cccaaggga acagctccg tccccactt cccacagcc gcatccaca gccagttct gggtgcccc ctcgaactt actacccgt tccatccac accctcacg tcgatttcg agcattcga tccaccaca cacacagtc cttcggtag acggcgcca gtcgcaagg caatcaacc ccgccggtc cgatgccat accgagaga cggatcttc tggaaggag ggacgtttc cgcggaaaa accaacgat tcgtagtgc cctcgaacc cgagcatca cttcgaaag tcagtaccg ggagtatag aacgcagct cggaacggg gcagtccca tgcccattt ttcacgtcg aagttttga gcccgggcg aggccggga gtagcggca aagagtgct gttcgtggg tcggaggcc agtataagc cgctcccgg gggaacttc gacgcgtgc acatcgaca aatcgaacg aaccgttag gaattcaaa tcaattgtc gcgaaagtg gcgggattt ttgtgtcaa ctgtgaatg ataatagtt tagcgagtc gcgtggggt tgcaaatcg gggaccagc tgctggagg tgtgtggga ttaatatga ggaacgcca cgtataacc tggcggcga atgtgttgc gacagtgcg ggaattcca tcactatgc tggtgcaat acagccctg ataaataca acgaattgg aagggtcag gttcttgtt ccggctcta gttcaaacg acgacgaag gtgatggcg aaaccacac cgtgtaact cgcccaagg aaatccgaa aatctcatc cagttggca taactaagt taccaccgc ccacttcgt tattaaggg ttgggcagc agacctcct cactacaaa ggcaaaata acagctcca gggacgatg atgagcccc gttttctct acatcgaga cgctgaaaa cgtccaatt tggggattt ccctagtcg ggggcaacg ccgctggca tttttattc attcggtcc agcccgatt ctctagcat accatgccg gtttacgca ccggtgacc aaatcctgg aatacaacg ggagcgacc tccgcaatg ccacggccg aagaagcgg cctacgaat tggccaaac cggccgata aagtcaccg tttcggccc actaccgca tcgatagat acaacgaaa tcaaagaca aaccgggcg atagtttgt acgtccgtt gcggtttcg accgctcgg gaaacgaac acacggaac cacctcaac tcgcttttg ccaaagacg atgtcctct atgtcgaca atacgatgt ttaacggag ttccaggtc aatggcgtg cttggaggt tggataatg agggtcgcc gccagcagt gcggagtga tccctagta aatataagg ttgaggagg agatgctcc tacggagag gtgccgatt tggaccaaa ccggacgcc cggtttcgg tcggatcgt ccatagccc gtcggagtt tcttccggc ggaaaaagc accaacgcc attcgaaag aactggcca gtttttgca gcctcgcgg gacctgggt ggtactccg acggcgggt ctttgcacg aggagccgg cgcctcccc cggcttacc agcgcgtgg agaggctgc ctttccccg agtttcggc cggttttgg tgctgggcc ctttggccg aatgtgtgg tggataaat tagtgagtg atttcccgg atgagttcg ggcgggcac ctgcagaga cccggcgct gctcccagg cgcagcttg accgggagt tggccgatg gccacctgg tggagtacc gccgacggg ggagctgct gggagtgcg tcactgtgg ccgcagtga ggcaagtct ccagagctc acctccatt gtatgttgg acgtggcgg caagcagtg tcgagcggc tccatcggc atggtatat acccggtgg tactcctca tcaagttca agagtacca agcaaatcc gggaggtga aggacgccc tgaggtaca gcttggata agttgtcgg gcaaagcag cgaaggaga tgttcgagc atgggcata aacttgaag ctgagtacc ggcatttgg tgaccgctg ttgtagcag ctggagcga atgtggcac atgtttgtg ctcaggtta aagccgccg ttgatgcgg aacatagga agagtcagt gggttccgg tcggctgat tcatgtcaa ttaaatatg tgcctattt ttcactttt ataacattt gtactcata cttgataat tagttttgc ttgaattta caggataca taatcagca gttttgtta aatttataa ctgttttag ctcgcactg ttaatcagt attttatat tttattttt tgactttat tatattgta catatacag tttaggtaa tctaaggtg gttgtatat tgtttcttt actgtgatt attttattg ttcttatat ttcttttaa agaaaataa atgtttcaa acatta
