Status
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Gene information
General information
TC006425 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9015944..9022878
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006425
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06215

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006425 RA
      MASNSDGSG SNGTSPRNL SINASRDYD TLKQQYEKA 
      NSELSNSRR RCDHALNDL DYFREQHRA AMNQLEVSS 
      QDSASLRTR YSELLSEKQ RLEQENQRL RKELNEVHR 
      QQQDVVLLA ENGNSDSLY ISHSQALSK LELAKDENN 
      RLAKQFEQE RNNAQREID SLKQQVLKY YKAHQKVQQ 
      QYEEAEKQI VAIKIKANK EVKRLTDER NATLAEYTL 
      VMSERDQVH KEMEKLSDD LAQALKKGK MLDADNQEL 
      VGEKQAIGY QLESLRREI ASALYERDK ALKECADLR 
      EKFGELGAS NDDNRRREQ TRSRHLEGL GGPCENVGR 
      KDALQSDVR TIGQRQRLD NLDQANQEL ESLRKSLDK 
      AQTELSEAI QEAEVSKGR RDWAFSERD KIVQERESI 
      RTLCDNMRK ERDRAVSEL AESLRESDA LKKQRNELL 
      KEVKALKEM LEAHIVDHD DDHFSDIDD STEILEVEL 
      TCDGASDLG LELAGGRDD PHFPGDGAV YVAAVAEGG 
      IADGKLRPN DRISRVNDV DCSAVSRRM VIEAIRASL 
      PVARIVVSR RRRRGPSPS PSIASQVTK WVHTARLVP 
      GCHGLTLNT GVYISRISD GSLAAKDKS LSVGDRILR 
      INEKSMDDI ESIHEAMRV LNDDSVDVI TVTALKISH 
      PECPNVFTP VRRHKRENR SSQTDEHDY LSNQQEKNN 
      GGAWFKEKI DLMRGRRHA KDNDKKKYR NSSPSPLEL 
      ADPEQAIAE LDLVLDSYR GNANVGTVK RTKRGTKEQ 
      EKNGGTWPK AKATALLQP VLGSTVVVR NKERPPLSL 
      LKEAGAEYH RNSTPVPLP IASVGPRAT NRHSVYKSV 
      DGGGSHFST TGDSFEKLR LAQSNRLSA NMNSDNSLD 
      YPVHRYADK DIHTYYKKN KLGKYAFDC DRDSVVGHT 
      ESSQGNSSV PTSHSRIHS QFWVPPRTY YPFHPHPHV 
      DFEHSIHHT HSPSVDGAS RKAINPAGP MPYRETDLL 
      EGGTFPRKN QRFVVPSNP SITSKVSTG SIERSSERG 
      SPMPIFHVE VLSPGEAGS SGKECCSWV GGQYKPLPG 
      ELRRVHIDK SNEPLGIQI NCRESGGIF VSTVNDNSL 
      ASRVGLQIG DQLLEVCGI NMRNATYNL AANVLRQCG 
      NSITMLVQY SPDKYNELE GSGSCSGSS SNDDEGDGE 
      TTPCNSPKE IRKSHPVGI TKLPPPTSL LRVGQQTSS 
      LQRQNNSSR DDDEPRFLY IETLKTSNL GISLVGGNA 
      AGIFIHSVQ PDSLAYHAG LRTGDQILE YNGSDLRNA 
      TAEEAAYEL AKPADKVTV SAHYRIDRY NEIKDKPGD 
      SLYVRCGFD RSGNEHTEP PQLAFAKDD VLYVDNTMF 
      NGVPGQWRA WRLDNEGRR QQCGVIPSK YKVEEEMLL 
      RRGADLDQT GRPVSVGSS IARRSFFRR KKHQRHSKE 
      LASFCSLAG PGWYSDGGS LHEEPAPPP AYQRVERLP 
      FPEFRPVLV LGPLAECVV DKLVSDFPD EFGRAPAET 
      RRCSQAQLD RELADGHLV EYRRRGSCW ECVTVAAVR 
      QVSRAHLHC MLDVAASSV ERLHRHGIY PVVLLIKFK 
      STKQIREVK DALRYSLDK LSGKAAKEM FEHGHKLEA 
      EYRHLVTAV VAAGANVAH VCAQVKAAV DAEHRKSQW 
      VPVG
    CDS sequence
    • >TC006425 RA
      atggcgtca aatagcgat ggtagtgga agcaatggt 
      acttcgccg agaaactta agcataaat gcttcaaga 
      gactatgac acactcaaa caacaatac gaaaaagct 
      aacagtgaa ttaagcaac tcgagaaga cgttgcgat 
      catgctctc aatgatctg gactacttt agggaacaa 
      cacagagcg gcaatgaac cagctagaa gtctcgtct 
      caagactcg gcctctctc agaacaaga tactcagaa 
      ttgttaagc gaaaaacag agattagaa caagaaaat 
      cagagatta agaaaagag ttaaatgag gtgcaccga 
      caacagcag gatgttgtt ttgcttgca gagaatgga 
      aattcagat tctttatac atttcacat tcacaggct 
      ttatcaaag ttggaactg gcaaaagat gagaataac 
      cggttagcg aagcagttt gaacaggaa agaaacaac 
      gcacaacgt gagatcgac agtttgaag caacaggtg 
      ctcaaatac tacaaagcc caccagaaa gtccaacaa 
      caatatgag gaagccgag aagcagatc gttgcaata 
      aaaatcaaa gcaaacaaa gaagtgaaa cgcttaacc 
      gacgaacgg aacgcaact ctagccgaa tacacttta 
      gtcatgagc gagagagac caagtccac aaagaaatg 
      gaaaaattg agcgacgac ttggcccaa gccctgaaa 
      aagggcaaa atgttggac gccgacaac caagaacta 
      gtcggcgag aaacaagca attgggtac cagttagaa 
      tcgctccgg cgcgaaatc gcaagcgcc ctctacgaa 
      cgcgataaa gccttaaag gagtgcgcc gaccttcgc 
      gaaaagttc ggcgagttg ggggcctcc aacgacgat 
      aatcgccgg agggagcag acgaggtcg cggcacttg 
      gaagggttg ggaggtcct tgcgaaaat gtgggtcgg 
      aaggatgcc ctccaatcg gacgtgcgg acgattggc 
      caaaggcaa aggcttgat aatttggac caagccaat 
      caagagttg gaatcgcta cgcaagagt ttagataag 
      gcgcagact gagttatcg gaggccatc caagaggcg 
      gaagtgtcg aaagggaga agggactgg gctttttcc 
      gagcgtgat aaaatcgtc caagagcgc gaaagcata 
      aggactttg tgtgataac atgcggaag gaacgcgat 
      cgggccgtt tcggaactt gccgaatcg ctccgggag 
      tcggacgcg ttgaagaag caacggaac gagctcctt 
      aaggaagtc aaggcttta aaggagatg cttgaggcg 
      catattgtt gatcatgat gacgatcat ttcagcgat 
      attgacgac agtaccgag atattggag gttgagttg 
      acttgtgac ggggcgtct gatttggga ttagagttg 
      gctgggggt cgtgatgat ccccatttt ccgggagac 
      ggcgctgtg tatgtcgct gctgtggcg gaaggcgga 
      atagccgac gggaaattg cgaccaaac gatcgcatt 
      tccagagtc aacgatgtc gattgtagt gctgtctca 
      cggcggatg gtcattgaa gctattagg gctagtctc 
      cctgtggct agaatcgtg gttagtcgg agacgccga 
      cgggggcct agcccttca cctagtatc gcttcacag 
      gtgaccaaa tgggttcat accgctcgt ttggttcct 
      ggctgccac ggtcttacc ctaaacacc ggggtttac 
      attagtaga attagcgac gggagtctc gccgctaaa 
      gacaagagt ctctcagtc ggggaccga atactccga 
      atcaacgaa aaatcaatg gatgatata gaaagcatc 
      catgaagcc atgcgagtc cttaacgac gactccgtc 
      gacgttatc accgtcaca gcgctgaag ataagccac 
      cccgagtgt cccaacgtt ttcactcct gtccgccgt 
      cacaagcgc gaaaatcgt agctcgcag acggacgaa 
      cacgattat ctctccaac cagcaagag aaaaacaac 
      ggcggggcg tggtttaaa gagaagatt gatctgatg 
      agaggtaga cgtcatgcc aaagataac gataagaaa 
      aagtatcgg aacagttcg cccagtcct ttggaattg 
      gccgatccg gagcaagcc atcgccgaa ctggacttg 
      gttttggac agttataga gggaatgcc aacgtcgga 
      actgtgaaa cgaacgaaa cgagggacg aaggaacag 
      gagaagaat ggaggaacc tggcccaag gcgaaagcc 
      acggcgttg cttcagcca gtccttgga agtaccgtg 
      gtggtacgc aacaaggaa cggcctcct ctgagtttg 
      ctgaaggag gcaggcgcc gaatatcac agaaactcc 
      actcctgtg ccactgccc atagcttcg gtgggtccg 
      cgggcgacc aaccgccac tccgtctac aaaagcgta 
      gacggcggt ggttcccac tttagcacc accggggat 
      tccttcgaa aaacttcgt ttagcccaa tcaaaccgc 
      ctaagtgcc aacatgaac tcggacaac agtctcgac 
      taccccgtc catcgctac gccgataag gacatccac 
      acctactac aagaaaaac aagctcggg aaatacgcc 
      tttgactgt gatcgcgac agtgtcgtc ggccacact 
      gagtcttcc caagggaac agctccgtc cccacttcc 
      cacagccgc atccacagc cagttctgg gtgccccct 
      cgaacttac tacccgttc catccacac cctcacgtc 
      gatttcgag cattcgatc caccacaca cacagtcct 
      tcggtagac ggcgccagt cgcaaggca atcaacccc 
      gccggtccg atgccatac cgagagacg gatcttctg 
      gaaggaggg acgtttccg cggaaaaac caacgattc 
      gtagtgccc tcgaacccg agcatcact tcgaaagtc 
      agtaccggg agtatagaa cgcagctcg gaacggggc 
      agtcccatg cccattttt cacgtcgaa gttttgagc 
      ccgggcgag gccgggagt agcggcaaa gagtgctgt 
      tcgtgggtc ggaggccag tataagccg ctcccgggg 
      gaacttcga cgcgtgcac atcgacaaa tcgaacgaa 
      ccgttagga attcaaatc aattgtcgc gaaagtggc 
      gggattttt gtgtcaact gtgaatgat aatagttta 
      gcgagtcgc gtggggttg caaatcggg gaccagctg 
      ctggaggtg tgtgggatt aatatgagg aacgccacg 
      tataacctg gcggcgaat gtgttgcga cagtgcggg 
      aattccatc actatgctg gtgcaatac agccctgat 
      aaatacaac gaattggaa gggtcaggt tcttgttcc 
      ggctctagt tcaaacgac gacgaaggt gatggcgaa 
      accacaccg tgtaactcg cccaaggaa atccgaaaa 
      tctcatcca gttggcata actaagtta ccaccgccc 
      acttcgtta ttaagggtt gggcagcag acctcctca 
      ctacaaagg caaaataac agctccagg gacgatgat 
      gagccccgt tttctctac atcgagacg ctgaaaacg 
      tccaatttg gggatttcc ctagtcggg ggcaacgcc 
      gctggcatt tttattcat tcggtccag cccgattct 
      ctagcatac catgccggt ttacgcacc ggtgaccaa 
      atcctggaa tacaacggg agcgacctc cgcaatgcc 
      acggccgaa gaagcggcc tacgaattg gccaaaccg 
      gccgataaa gtcaccgtt tcggcccac taccgcatc 
      gatagatac aacgaaatc aaagacaaa ccgggcgat 
      agtttgtac gtccgttgc ggtttcgac cgctcggga 
      aacgaacac acggaacca cctcaactc gcttttgcc 
      aaagacgat gtcctctat gtcgacaat acgatgttt 
      aacggagtt ccaggtcaa tggcgtgct tggaggttg 
      gataatgag ggtcgccgc cagcagtgc ggagtgatc 
      cctagtaaa tataaggtt gaggaggag atgctccta 
      cggagaggt gccgatttg gaccaaacc ggacgcccg 
      gtttcggtc ggatcgtcc atagcccgt cggagtttc 
      ttccggcgg aaaaagcac caacgccat tcgaaagaa 
      ctggccagt ttttgcagc ctcgcggga cctgggtgg 
      tactccgac ggcgggtct ttgcacgag gagccggcg 
      cctcccccg gcttaccag cgcgtggag aggctgcct 
      ttccccgag tttcggccg gttttggtg ctgggccct 
      ttggccgaa tgtgtggtg gataaatta gtgagtgat 
      ttcccggat gagttcggg cgggcacct gcagagacc 
      cggcgctgc tcccaggcg cagcttgac cgggagttg 
      gccgatggc cacctggtg gagtaccgc cgacggggg 
      agctgctgg gagtgcgtc actgtggcc gcagtgagg 
      caagtctcc agagctcac ctccattgt atgttggac 
      gtggcggca agcagtgtc gagcggctc catcggcat 
      ggtatatac ccggtggta ctcctcatc aagttcaag 
      agtaccaag caaatccgg gaggtgaag gacgccctg 
      aggtacagc ttggataag ttgtcgggc aaagcagcg 
      aaggagatg ttcgagcat gggcataaa cttgaagct 
      gagtaccgg catttggtg accgctgtt gtagcagct 
      ggagcgaat gtggcacat gtttgtgct caggttaaa 
      gccgccgtt gatgcggaa cataggaag agtcagtgg 
      gttccggtc ggctga
    mRNA sequence
    • >TC006425 RA
      tggtaaaag acgaagtag cagtaaaga tggtggtta 
      gtagttaaa ttttaaatt aaattagga ataaccaaa 
      tgtgatgat tcgtgaatt cacggcatc ctgagggtg 
      tgatggcgt caaatagcg atggtagtg gaagcaatg 
      gtacttcgc cgagaaact taagcataa atgcttcaa 
      gagactatg acacactca aacaacaat acgaaaaag 
      ctaacagtg aattaagca actcgagaa gacgttgcg 
      atcatgctc tcaatgatc tggactact ttagggaac 
      aacacagag cggcaatga accagctag aagtctcgt 
      ctcaagact cggcctctc tcagaacaa gatactcag 
      aattgttaa gcgaaaaac agagattag aacaagaaa 
      atcagagat taagaaaag agttaaatg aggtgcacc 
      gacaacagc aggatgttg ttttgcttg cagagaatg 
      gaaattcag attctttat acatttcac attcacagg 
      ctttatcaa agttggaac tggcaaaag atgagaata 
      accggttag cgaagcagt ttgaacagg aaagaaaca 
      acgcacaac gtgagatcg acagtttga agcaacagg 
      tgctcaaat actacaaag cccaccaga aagtccaac 
      aacaatatg aggaagccg agaagcaga tcgttgcaa 
      taaaaatca aagcaaaca aagaagtga aacgcttaa 
      ccgacgaac ggaacgcaa ctctagccg aatacactt 
      tagtcatga gcgagagag accaagtcc acaaagaaa 
      tggaaaaat tgagcgacg acttggccc aagccctga 
      aaaagggca aaatgttgg acgccgaca accaagaac 
      tagtcggcg agaaacaag caattgggt accagttag 
      aatcgctcc ggcgcgaaa tcgcaagcg ccctctacg 
      aacgcgata aagccttaa aggagtgcg ccgaccttc 
      gcgaaaagt tcggcgagt tgggggcct ccaacgacg 
      ataatcgcc ggagggagc agacgaggt cgcggcact 
      tggaagggt tgggaggtc cttgcgaaa atgtgggtc 
      ggaaggatg ccctccaat cggacgtgc ggacgattg 
      gccaaaggc aaaggcttg ataatttgg accaagcca 
      atcaagagt tggaatcgc tacgcaaga gtttagata 
      aggcgcaga ctgagttat cggaggcca tccaagagg 
      cggaagtgt cgaaaggga gaagggact gggcttttt 
      ccgagcgtg ataaaatcg tccaagagc gcgaaagca 
      taaggactt tgtgtgata acatgcgga aggaacgcg 
      atcgggccg tttcggaac ttgccgaat cgctccggg 
      agtcggacg cgttgaaga agcaacgga acgagctcc 
      ttaaggaag tcaaggctt taaaggaga tgcttgagg 
      cgcatattg ttgatcatg atgacgatc atttcagcg 
      atattgacg acagtaccg agatattgg aggttgagt 
      tgacttgtg acggggcgt ctgatttgg gattagagt 
      tggctgggg gtcgtgatg atccccatt ttccgggag 
      acggcgctg tgtatgtcg ctgctgtgg cggaaggcg 
      gaatagccg acgggaaat tgcgaccaa acgatcgca 
      tttccagag tcaacgatg tcgattgta gtgctgtct 
      cacggcgga tggtcattg aagctatta gggctagtc 
      tccctgtgg ctagaatcg tggttagtc ggagacgcc 
      gacgggggc ctagccctt cacctagta tcgcttcac 
      aggtgacca aatgggttc ataccgctc gtttggttc 
      ctggctgcc acggtctta ccctaaaca ccggggttt 
      acattagta gaattagcg acgggagtc tcgccgcta 
      aagacaaga gtctctcag tcggggacc gaatactcc 
      gaatcaacg aaaaatcaa tggatgata tagaaagca 
      tccatgaag ccatgcgag tccttaacg acgactccg 
      tcgacgtta tcaccgtca cagcgctga agataagcc 
      accccgagt gtcccaacg ttttcactc ctgtccgcc 
      gtcacaagc gcgaaaatc gtagctcgc agacggacg 
      aacacgatt atctctcca accagcaag agaaaaaca 
      acggcgggg cgtggttta aagagaaga ttgatctga 
      tgagaggta gacgtcatg ccaaagata acgataaga 
      aaaagtatc ggaacagtt cgcccagtc ctttggaat 
      tggccgatc cggagcaag ccatcgccg aactggact 
      tggttttgg acagttata gagggaatg ccaacgtcg 
      gaactgtga aacgaacga aacgaggga cgaaggaac 
      aggagaaga atggaggaa cctggccca aggcgaaag 
      ccacggcgt tgcttcagc cagtccttg gaagtaccg 
      tggtggtac gcaacaagg aacggcctc ctctgagtt 
      tgctgaagg aggcaggcg ccgaatatc acagaaact 
      ccactcctg tgccactgc ccatagctt cggtgggtc 
      cgcgggcga ccaaccgcc actccgtct acaaaagcg 
      tagacggcg gtggttccc actttagca ccaccgggg 
      attccttcg aaaaacttc gtttagccc aatcaaacc 
      gcctaagtg ccaacatga actcggaca acagtctcg 
      actaccccg tccatcgct acgccgata aggacatcc 
      acacctact acaagaaaa acaagctcg ggaaatacg 
      cctttgact gtgatcgcg acagtgtcg tcggccaca 
      ctgagtctt cccaaggga acagctccg tccccactt 
      cccacagcc gcatccaca gccagttct gggtgcccc 
      ctcgaactt actacccgt tccatccac accctcacg 
      tcgatttcg agcattcga tccaccaca cacacagtc 
      cttcggtag acggcgcca gtcgcaagg caatcaacc 
      ccgccggtc cgatgccat accgagaga cggatcttc 
      tggaaggag ggacgtttc cgcggaaaa accaacgat 
      tcgtagtgc cctcgaacc cgagcatca cttcgaaag 
      tcagtaccg ggagtatag aacgcagct cggaacggg 
      gcagtccca tgcccattt ttcacgtcg aagttttga 
      gcccgggcg aggccggga gtagcggca aagagtgct 
      gttcgtggg tcggaggcc agtataagc cgctcccgg 
      gggaacttc gacgcgtgc acatcgaca aatcgaacg 
      aaccgttag gaattcaaa tcaattgtc gcgaaagtg 
      gcgggattt ttgtgtcaa ctgtgaatg ataatagtt 
      tagcgagtc gcgtggggt tgcaaatcg gggaccagc 
      tgctggagg tgtgtggga ttaatatga ggaacgcca 
      cgtataacc tggcggcga atgtgttgc gacagtgcg 
      ggaattcca tcactatgc tggtgcaat acagccctg 
      ataaataca acgaattgg aagggtcag gttcttgtt 
      ccggctcta gttcaaacg acgacgaag gtgatggcg 
      aaaccacac cgtgtaact cgcccaagg aaatccgaa 
      aatctcatc cagttggca taactaagt taccaccgc 
      ccacttcgt tattaaggg ttgggcagc agacctcct 
      cactacaaa ggcaaaata acagctcca gggacgatg 
      atgagcccc gttttctct acatcgaga cgctgaaaa 
      cgtccaatt tggggattt ccctagtcg ggggcaacg 
      ccgctggca tttttattc attcggtcc agcccgatt 
      ctctagcat accatgccg gtttacgca ccggtgacc 
      aaatcctgg aatacaacg ggagcgacc tccgcaatg 
      ccacggccg aagaagcgg cctacgaat tggccaaac 
      cggccgata aagtcaccg tttcggccc actaccgca 
      tcgatagat acaacgaaa tcaaagaca aaccgggcg 
      atagtttgt acgtccgtt gcggtttcg accgctcgg 
      gaaacgaac acacggaac cacctcaac tcgcttttg 
      ccaaagacg atgtcctct atgtcgaca atacgatgt 
      ttaacggag ttccaggtc aatggcgtg cttggaggt 
      tggataatg agggtcgcc gccagcagt gcggagtga 
      tccctagta aatataagg ttgaggagg agatgctcc 
      tacggagag gtgccgatt tggaccaaa ccggacgcc 
      cggtttcgg tcggatcgt ccatagccc gtcggagtt 
      tcttccggc ggaaaaagc accaacgcc attcgaaag 
      aactggcca gtttttgca gcctcgcgg gacctgggt 
      ggtactccg acggcgggt ctttgcacg aggagccgg 
      cgcctcccc cggcttacc agcgcgtgg agaggctgc 
      ctttccccg agtttcggc cggttttgg tgctgggcc 
      ctttggccg aatgtgtgg tggataaat tagtgagtg 
      atttcccgg atgagttcg ggcgggcac ctgcagaga 
      cccggcgct gctcccagg cgcagcttg accgggagt 
      tggccgatg gccacctgg tggagtacc gccgacggg 
      ggagctgct gggagtgcg tcactgtgg ccgcagtga 
      ggcaagtct ccagagctc acctccatt gtatgttgg 
      acgtggcgg caagcagtg tcgagcggc tccatcggc 
      atggtatat acccggtgg tactcctca tcaagttca 
      agagtacca agcaaatcc gggaggtga aggacgccc 
      tgaggtaca gcttggata agttgtcgg gcaaagcag 
      cgaaggaga tgttcgagc atgggcata aacttgaag 
      ctgagtacc ggcatttgg tgaccgctg ttgtagcag 
      ctggagcga atgtggcac atgtttgtg ctcaggtta 
      aagccgccg ttgatgcgg aacatagga agagtcagt 
      gggttccgg tcggctgat tcatgtcaa ttaaatatg 
      tgcctattt ttcactttt ataacattt gtactcata 
      cttgataat tagttttgc ttgaattta caggataca 
      taatcagca gttttgtta aatttataa ctgttttag 
      ctcgcactg ttaatcagt attttatat tttattttt 
      tgactttat tatattgta catatacag tttaggtaa 
      tctaaggtg gttgtatat tgtttcttt actgtgatt 
      attttattg ttcttatat ttcttttaa agaaaataa 
      atgtttcaa acatta
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