Status
Please wait ...
Gene information
General information
TC033981
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005978
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4262103..4263921
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033981
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06207

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    antenna distalcolour lighter - Number of animals showing the phenotype: 3


    legs tarsus sometimes not present - Number of animals showing the phenotype: 2


    legs tibia sometimes not present - Number of animals showing the phenotype: 3


    legs colour lighter - Number of animals showing the phenotype: 8


    elytra need termsurface irregular - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033981 RA
      MENPLQLRN ESQLMQVEF TSFQPRMYR CCCRRRFPL 
      QRENNPLFR AVLSDNLAE LQTLVESGH RVNTKNAKG 
      CSLLNFAMR RKRVEIFDY LLSLDQIDV DYRDRMHQS 
      PLLYLAGVC FDEYFALEL LKKGADVNL TDDSGFSFL 
      HLAARAYCD DNLRVGQLV IDKGADVDL RGHYGVTPL 
      YAACEEGNF DWVSMLLYY GADPMVPTH RKILPVAVV 
      ASNANQDEE QQTLLQEII FNHTFDNDK ICLSLPTLT 
      AAMTLSSDL FVKILDKAS SVEYDLSDL QHLVTSLIH 
      VKAQHLELF MQNFGFIIK DMIDNFPPL EVLIKISLD 
      CEMSGLRDI VQVFLDSEY CAEFVQSCD PTLPAISRL 
      IQEFTIEEE EKFTQMVCL MLTYGLGVT STDLDAVYN 
      QYGYCELFK LLLHMAVTL TNNCNNVHS IAALYYNPN 
      LSLDDFLND FSINSHLLH FYNHRKLKE ICLDVIEDG 
      EKLPELPQV PYLLELSRN AARNFIVSK FKIRNCSQF 
      YTVLRWLPI GEMNKKIIA LEKKIYE
    CDS sequence
    • >TC033981 RA
      atggaaaat cccttacaa cttcgtaat gaaagccag 
      ctcatgcag gtcgaattc acctcgttc caaccccgg 
      atgtaccgc tgttgttgc cgtcgtcgg ttcccgttg 
      cagcgcgag aacaacccc ctgttccgg gccgtcctg 
      tccgacaac ttagccgaa ctgcaaacc ctagtcgag 
      tctggccac cgcgtcaac accaaaaat gccaaaggc 
      tgctcgctc cttaacttc gcaatgcgc cgaaaacga 
      gtagaaatt ttcgattat ttgctaagt ctcgatcaa 
      attgacgtg gattaccgc gaccggatg caccaaagc 
      ccgcttttg tacctcgca ggagtgtgt ttcgacgaa 
      tatttcgcc ctagaattg cttaaaaaa ggggccgat 
      gttaatctg accgatgat tctgggttc agttttttg 
      cacctagct gcgcgggca tattgcgac gataattta 
      agggtgggt cagctggtc atagacaag ggggccgat 
      gtcgacctg cgggggcac tacggggtg actccgctg 
      tacgcagcc tgcgaggaa ggtaatttt gactgggtg 
      tccatgctg ttgtattat ggggcggac cccatggtc 
      cccacccat cggaaaata ctgccagtg gctgtggtg 
      gcgtcaaat gcgaaccag gacgaggag caacaaacc 
      ctcctccag gaaattatt tttaatcac accttcgac 
      aacgataaa atttgttta agtcttccc actttaaca 
      gctgccatg accttaagt tcggacctt tttgtaaaa 
      attcttgat aaagcttca agtgttgag tatgatttg 
      tccgatttg caacatttg gtcacttcg ctgattcat 
      gtgaaagcg caacacttg gaattgttt atgcaaaat 
      ttcggattt attattaaa gacatgatc gataatttc 
      cctccgttg gaggtcttg attaaaatt tcgcttgac 
      tgtgaaatg tcggggttg agggacata gtccaggtg 
      ttcctggat agcgaatac tgtgctgaa tttgtgcag 
      tcttgtgac ccgacttta cccgcaatt tcccgcttg 
      attcaagaa tttacaatc gaagaggaa gaaaagttc 
      actcaaatg gtttgcctt atgcttact tacggtctt 
      ggcgttacg tccaccgac ttggacgcg gtgtacaat 
      caatacggt tactgtgag ctctttaaa ttgttacta 
      cacatggcc gttacctta actaataac tgtaacaac 
      gtgcactca atcgctgcg ttatattac aatcctaac 
      ttgtctctt gatgacttt ctaaacgat ttttcgata 
      aactcgcat ttgttacac ttttacaat caccgcaaa 
      ttaaaggaa atttgttta gatgtgata gaagatggc 
      gaaaaactg ccagagtta ccacaagta ccttattta 
      ctagaattg agccgaaat gcagctcga aatttcata 
      gtcagtaaa ttcaaaatt aggaattgt agccaattc 
      tacacagtg ttgagatgg cttccgatt ggcgaaatg 
      aacaaaaaa attatcgct ctagagaag aaaatttac 
      gagtag
    mRNA sequence
    • >TC033981 RA
      acagtcagc tgatcactg acagctgtc agtgtcact 
      tgacaaaga taaataaat aatatggaa aatccctta 
      caacttcgt aatgaaagc cagctcatg caggtcgaa 
      ttcacctcg ttccaaccc cggatgtac cgctgttgt 
      tgccgtcgt cggttcccg ttgcagcgc gagaacaac 
      cccctgttc cgggccgtc ctgtccgac aacttagcc 
      gaactgcaa accctagtc gagtctggc caccgcgtc 
      aacaccaaa aatgccaaa ggctgctcg ctccttaac 
      ttcgcaatg cgccgaaaa cgagtagaa attttcgat 
      tatttgcta agtctcgat caaattgac gtggattac 
      cgcgaccgg atgcaccaa agcccgctt ttgtacctc 
      gcaggagtg tgtttcgac gaatatttc gccctagaa 
      ttgcttaaa aaaggggcc gatgttaat ctgaccgat 
      gattctggg ttcagtttt ttgcaccta gctgcgcgg 
      gcatattgc gacgataat ttaagggtg ggtcagctg 
      gtcatagac aagggggcc gatgtcgac ctgcggggg 
      cactacggg gtgactccg ctgtacgca gcctgcgag 
      gaaggtaat tttgactgg gtgtccatg ctgttgtat 
      tatggggcg gaccccatg gtccccacc catcggaaa 
      atactgcca gtggctgtg gtggcgtca aatgcgaac 
      caggacgag gagcaacaa accctcctc caggaaatt 
      atttttaat cacaccttc gacaacgat aaaatttgt 
      ttaagtctt cccacttta acagctgcc atgacctta 
      agttcggac ctttttgta aaaattctt gataaagct 
      tcaagtgtt gagtatgat ttgtccgat ttgcaacat 
      ttggtcact tcgctgatt catgtgaaa gcgcaacac 
      ttggaattg tttatgcaa aatttcgga tttattatt 
      aaagacatg atcgataat ttccctccg ttggaggtc 
      ttgattaaa atttcgctt gactgtgaa atgtcgggg 
      ttgagggac atagtccag gtgttcctg gatagcgaa 
      tactgtgct gaatttgtg cagtcttgt gacccgact 
      ttacccgca atttcccgc ttgattcaa gaatttaca 
      atcgaagag gaagaaaag ttcactcaa atggtttgc 
      cttatgctt acttacggt cttggcgtt acgtccacc 
      gacttggac gcggtgtac aatcaatac ggttactgt 
      gagctcttt aaattgtta ctacacatg gccgttacc 
      ttaactaat aactgtaac aacgtgcac tcaatcgct 
      gcgttatat tacaatcct aacttgtct cttgatgac 
      tttctaaac gatttttcg ataaactcg catttgtta 
      cacttttac aatcaccgc aaattaaag gaaatttgt 
      ttagatgtg atagaagat ggcgaaaaa ctgccagag 
      ttaccacaa gtaccttat ttactagaa ttgagccga 
      aatgcagct cgaaatttc atagtcagt aaattcaaa 
      attaggaat tgtagccaa ttctacaca gtgttgaga 
      tggcttccg attggcgaa atgaacaaa aaaattatc 
      gctctagag aagaaaatt tacgagtag gattgttac 
      gaaatgtgc cttcgaaaa tgtgatctc tgattgttt 
      taattgcgc aacaaagtg tttgcttgt tttacttta 
      cagtgtaat attttcctg taacttgga ataataaat 
      tattcaaag taac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY