Status
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Gene information
General information
TC034824 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5726945..5731733
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034824
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06205

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034824 RA
      MTKVSDIPK TTKNDKRFG KYLVFLVTA GLILSIIGT 
      SIYFVFFHN ETIFRGAVV TNGGTCAQI GASILSKGG 
      TAVDAAIAA LLCDGVSVP NCMGIGGGF FMNIYTKDK 
      GVEFLDSRE VAPGAASEN MYGTNQSLA SRGGLAVAV 
      PGELKGYWE AYRKYGGKV PWKDLVQPS IDLCKSGIL 
      VTKYLEKML ALQVDVISK DEFLRETFI DPKTNKTYV 
      QNQYIKRPR LAKTLEIVA ESGPDALYN GTLTEGFLQ 
      DIRNKGGII TREDLINFK PQWKKPIQI QLPLNQTLY 
      TPPPPGSGV ILSLIMNIL NGFLDFSQP KLLTNWQRV 
      VESFKFGFA KRTTLGDPN FVPMDDFVG NLTSKWFAE 
      K
    • >TC034824 RB
      MFNRFGKYL VFLVTAGLI LSIIGTSIY FVFFHNETI 
      FRGAVVTNG GTCAQIGAS ILSKGGTAV DAAIAALLC 
      DGVSVPNCM GIGGGFFMN IYTKDKGVE FLDSREVAP 
      GAASENMYG TNQSLASRG GLAVAVPGE LKGYWEAYR 
      KYGGKVPWK DLVQPSIDL CKSGILVTK YLEKMLALQ 
      VDVISKDEF LRETFIDPK TNKTYVQNQ YIKRPRLAK 
      TLEIVAESG PDALYNGTL TEGFLQDIR NKGGIITRE 
      DLINFKPQW KKPIQIQLP LNQTLYTPP PPGSGVILS 
      LIMNILNGF LDFSQPKLL TNWQRVVES FKFGFAKRT 
      TLGDPNFVP MDDFVGNLT SKWFAEK
    CDS sequence
    • >TC034824 RA
      atgacaaaa gtgtcagac atacccaaa actacaaaa 
      aatgataaa agatttggc aaatacttg gttttcctc 
      gtcacagcc ggcttaatt ctgtccata atcggcaca 
      agcatttac tttgttttc ttccacaac gagacaata 
      tttcggggg gctgtagtg accaacggc ggcacatgt 
      gcccaaatc ggggcctcc atcctctcc aaagggggc 
      accgccgtc gacgccgcc atcgcggcc ctcttgtgt 
      gacggcgtc tcagtgccg aactgtatg gggatcggg 
      ggcggtttt ttcatgaac atttacacc aaggacaag 
      ggggttgag ttcctcgat tcgagggaa gtggcaccg 
      ggggctgcc agtgaaaac atgtacggc accaatcaa 
      agtctggcg agtcgaggg ggcctcgct gtggcggtg 
      cccggcgaa ctgaagggg tactgggag gcctacagg 
      aaatatggg ggcaaagtg ccgtggaag gatttggtg 
      caaccgtcg atagatttg tgcaaaagc gggattttg 
      gtcacgaaa tatttggaa aaaatgcta gcattgcaa 
      gtcgatgtt atttcaaaa gatgagttc cttcgggag 
      acttttatt gacccgaaa acgaacaaa acgtacgtc 
      caaaaccag tacattaag agacctaga ctcgccaaa 
      acgttagaa atcgtggct gagtccggt cctgatgct 
      ctttataat ggtactcta actgagggt tttttgcaa 
      gatatacgc aacaaaggt ggaataata acacgagaa 
      gatttgatt aatttcaag ccacagtgg aaaaaacca 
      atacaaatc caattacct ctaaaccag acactttac 
      acccctccg ccgccaggc tctggtgtt attttaagc 
      ctaattatg aacattttg aatgggttt ttagatttt 
      tcccaacct aagttgcta acaaactgg caacgagtc 
      gtcgaaagt ttcaaattt ggtttcgct aagagaaca 
      accttaggg gacccaaat tttgtccca atggatgac 
      tttgttggt aatcttacg tcgaaatgg ttcgccgag 
      aaatga
    • >TC034824 RB
      atgttcaac agatttggc aaatacttg gttttcctc 
      gtcacagcc ggcttaatt ctgtccata atcggcaca 
      agcatttac tttgttttc ttccacaac gagacaata 
      tttcggggg gctgtagtg accaacggc ggcacatgt 
      gcccaaatc ggggcctcc atcctctcc aaagggggc 
      accgccgtc gacgccgcc atcgcggcc ctcttgtgt 
      gacggcgtc tcagtgccg aactgtatg gggatcggg 
      ggcggtttt ttcatgaac atttacacc aaggacaag 
      ggggttgag ttcctcgat tcgagggaa gtggcaccg 
      ggggctgcc agtgaaaac atgtacggc accaatcaa 
      agtctggcg agtcgaggg ggcctcgct gtggcggtg 
      cccggcgaa ctgaagggg tactgggag gcctacagg 
      aaatatggg ggcaaagtg ccgtggaag gatttggtg 
      caaccgtcg atagatttg tgcaaaagc gggattttg 
      gtcacgaaa tatttggaa aaaatgcta gcattgcaa 
      gtcgatgtt atttcaaaa gatgagttc cttcgggag 
      acttttatt gacccgaaa acgaacaaa acgtacgtc 
      caaaaccag tacattaag agacctaga ctcgccaaa 
      acgttagaa atcgtggct gagtccggt cctgatgct 
      ctttataat ggtactcta actgagggt tttttgcaa 
      gatatacgc aacaaaggt ggaataata acacgagaa 
      gatttgatt aatttcaag ccacagtgg aaaaaacca 
      atacaaatc caattacct ctaaaccag acactttac 
      acccctccg ccgccaggc tctggtgtt attttaagc 
      ctaattatg aacattttg aatgggttt ttagatttt 
      tcccaacct aagttgcta acaaactgg caacgagtc 
      gtcgaaagt ttcaaattt ggtttcgct aagagaaca 
      accttaggg gacccaaat tttgtccca atggatgac 
      tttgttggt aatcttacg tcgaaatgg ttcgccgag 
      aaatga
    mRNA sequence
    • >TC034824 RA
      gagatctgc ttttattag tgtttctcg ttatcgcaa 
      aaatgttca acaggtaag tcacacgtt ttcttaaat 
      ttgtcaaat taatgacaa aagtgtcag acataccca 
      aaactacaa aaaatgata aaagatttg gcaaatact 
      tggttttcc tcgtcacag ccggcttaa ttctgtcca 
      taatcggca caagcattt actttgttt tcttccaca 
      acgagacaa tatttcggg gggctgtag tgaccaacg 
      gcggcacat gtgcccaaa tcggggcct ccatcctct 
      ccaaagggg gcaccgccg tcgacgccg ccatcgcgg 
      ccctcttgt gtgacggcg tctcagtgc cgaactgta 
      tggggatcg ggggcggtt ttttcatga acatttaca 
      ccaaggaca agggggttg agttcctcg attcgaggg 
      aagtggcac cgggggctg ccagtgaaa acatgtacg 
      gcaccaatc aaagtctgg cgagtcgag ggggcctcg 
      ctgtggcgg tgcccggcg aactgaagg ggtactggg 
      aggcctaca ggaaatatg ggggcaaag tgccgtgga 
      aggatttgg tgcaaccgt cgatagatt tgtgcaaaa 
      gcgggattt tggtcacga aatatttgg aaaaaatgc 
      tagcattgc aagtcgatg ttatttcaa aagatgagt 
      tccttcggg agactttta ttgacccga aaacgaaca 
      aaacgtacg tccaaaacc agtacatta agagaccta 
      gactcgcca aaacgttag aaatcgtgg ctgagtccg 
      gtcctgatg ctctttata atggtactc taactgagg 
      gttttttgc aagatatac gcaacaaag gtggaataa 
      taacacgag aagatttga ttaatttca agccacagt 
      ggaaaaaac caatacaaa tccaattac ctctaaacc 
      agacacttt acacccctc cgccgccag gctctggtg 
      ttattttaa gcctaatta tgaacattt tgaatgggt 
      ttttagatt tttcccaac ctaagttgc taacaaact 
      ggcaacgag tcgtcgaaa gtttcaaat ttggtttcg 
      ctaagagaa caaccttag gggacccaa attttgtcc 
      caatggatg actttgttg gtaatctta cgtcgaaat 
      ggttcgccg agaaatgag gaagcaaat tttcgacac 
      taaacgttc caaaccagt
    • >TC034824 RB
      gagatctgc ttttattag tgtttctcg ttatcgcaa 
      aaatgttca acagatttg gcaaatact tggttttcc 
      tcgtcacag ccggcttaa ttctgtcca taatcggca 
      caagcattt actttgttt tcttccaca acgagacaa 
      tatttcggg gggctgtag tgaccaacg gcggcacat 
      gtgcccaaa tcggggcct ccatcctct ccaaagggg 
      gcaccgccg tcgacgccg ccatcgcgg ccctcttgt 
      gtgacggcg tctcagtgc cgaactgta tggggatcg 
      ggggcggtt ttttcatga acatttaca ccaaggaca 
      agggggttg agttcctcg attcgaggg aagtggcac 
      cgggggctg ccagtgaaa acatgtacg gcaccaatc 
      aaagtctgg cgagtcgag ggggcctcg ctgtggcgg 
      tgcccggcg aactgaagg ggtactggg aggcctaca 
      ggaaatatg ggggcaaag tgccgtgga aggatttgg 
      tgcaaccgt cgatagatt tgtgcaaaa gcgggattt 
      tggtcacga aatatttgg aaaaaatgc tagcattgc 
      aagtcgatg ttatttcaa aagatgagt tccttcggg 
      agactttta ttgacccga aaacgaaca aaacgtacg 
      tccaaaacc agtacatta agagaccta gactcgcca 
      aaacgttag aaatcgtgg ctgagtccg gtcctgatg 
      ctctttata atggtactc taactgagg gttttttgc 
      aagatatac gcaacaaag gtggaataa taacacgag 
      aagatttga ttaatttca agccacagt ggaaaaaac 
      caatacaaa tccaattac ctctaaacc agacacttt 
      acacccctc cgccgccag gctctggtg ttattttaa 
      gcctaatta tgaacattt tgaatgggt ttttagatt 
      tttcccaac ctaagttgc taacaaact ggcaacgag 
      tcgtcgaaa gtttcaaat ttggtttcg ctaagagaa 
      caaccttag gggacccaa attttgtcc caatggatg 
      actttgttg gtaatctta cgtcgaaat ggttcgccg 
      agaaatgag gaagcaaat tttcgacac taaacgttc 
      caaaccagt
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