Status
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Gene information
General information
TC034017
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005874,TC005875
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6113837..6117912
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034017
    iBeetle screen iB_06204

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034017 RA
      MKTILCFTV LFLCANSQN LLNDFRRFF GASDVRDTA 
      NFRKSYDFV VVGAGSGGC VVANRLSEN PEWSVLLLE 
      AGDDENFLT DVPLIASLQ TITSYNWGY KSERLATAC 
      LGLIDGRCN MPRGKALGG TSVINFLLY TRGTKQDFD 
      QWAELGNPG WGYDQVLPY FIKSENCTK CREIDGKYH 
      GKSGYLSVE HPGYESPLV KRFIKSGEE LGYKNNDPS 
      APYGLGFSK VLATMRNGM RCSASKAFL KPILHRTNL 
      HVSIKTRVT KILIDPSTK QAYGVQFWK NRRKFTVLA 
      TKEVVLSAG SINSPHLLM LSGVGPRDD LTRVGIPLL 
      QNLKVGYNL QDHMAMSAL VFFVNESIT VSDRGVQNP 
      VDIFNYVFN GRGPYTIPG GAEALAFVQ TKYAKIGGY 
      PDIELVLGA GALNGDVYG SLRSLLGIP RSLFERVYA 
      PHAYKPAFS IAPVLMRPK SRGRVVIKD GNPLHWPKL 
      IPNYFENEE DVKTMVEGI KMARWSSSA SVFCSSAIT 
      ITQSRHFQK YNITMITTP FPGCETVPF GSDEYWACA 
      VRHVATTLG HQVGTCKMG PPSDPDAVV DERLRVYGI 
      KGLRVVDGS IMPNVVAGH TNAVIMMIG EKASDMIKQ 
      EWARK
    CDS sequence
    • >TC034017 RA
      atgaagaca atcctgtgt tttactgtg cttttcttg 
      tgtgcaaac tcccaaaac ctcctcaac gacttccgc 
      cgctttttc ggggcgagt gacgtccgc gacaccgcg 
      aattttcgc aaaagctac gactttgtg gtcgtgggg 
      gcgggctcc gggggctgc gtcgtcgcc aaccgtctg 
      agcgaaaac cccgagtgg tccgtcctg ttactggaa 
      gccggcgat gacgaaaat tttttgacc gatgtcccg 
      ctaattgcc tcgcttcaa accatcacg agctacaat 
      tggggctat aagagcgaa agactcgcg acggcctgt 
      ttggggctc atcgacggc cgctgtaac atgccgagg 
      ggcaaagcc ctggggggc acctccgtg atcaatttt 
      ttgctatac accaggggc accaagcag gactttgac 
      caatgggct gagttggga aacccaggc tgggggtac 
      gaccaagtc ctcccctat ttcatcaaa tcggaaaat 
      tgcaccaaa tgccgggaa attgacggg aaatatcac 
      gggaaaagc ggctacttg agtgtggaa cacccgggg 
      tatgagtca cccctagtc aagcgtttc ataaaatcc 
      ggggaagaa ctgggttac aagaacaac gaccctagc 
      gccccctat ggactgggt ttttccaaa gttttggca 
      acaatgagg aacgggatg cggtgcagt gcctctaag 
      gcgttcctc aagcccatt ttacaccgc acaaactta 
      cacgtgtcg attaaaaca cgtgtcacc aaaatccta 
      atcgatccg agcaccaaa caagcatac ggcgtccaa 
      ttttggaaa aatcgccga aaatttacc gtcctagcc 
      accaaagaa gtggttttg tcggccggt tcgatcaac 
      agcccccat ttactgatg ctgtcgggc gtgggcccc 
      cgggacgat ttaacccgt gtgggaatc cccctcttg 
      caaaattta aaagtcggg tataaccta caggaccat 
      atggcgatg tccgcactg gtgtttttc gtcaatgag 
      tcaatcact gtgtcggac cgtggggtg caaaacccc 
      gttgatatt tttaattat gtgtttaat ggaaggggg 
      ccctatact atacctggg ggggccgaa gccctggct 
      ttcgtgcag acgaagtat gcaaaaatc gggggttac 
      cccgatatt gaattggtg ttaggtgcc ggggcgctg 
      aatggcgat gtgtatgga agtttgagg agtcttttg 
      gggattccc cgaagtttg ttcgagagg gtttacgcc 
      ccacatgcg tacaaaccg gcgtttagt atcgcaccg 
      gttttgatg aggcccaaa agtcgaggc agggttgtg 
      atcaaagat gggaatccc ctgcattgg cctaaatta 
      ataccgaat tatttcgag aatgaggag gatgtcaaa 
      acaatggtc gaggggatc aagatggca aggtggtcc 
      agctcagct tccgtcttc tgtagctca gcgattaca 
      attacgcaa tctcgacat tttcaaaaa tacaacatt 
      acaatgatt acgacccca tttcctggg tgtgaaact 
      gtaccattt ggtagcgac gagtattgg gcttgtgcc 
      gttcggcac gttgccacc actctgggg caccaggtt 
      gggacctgc aaaatgggg cccccgagc gatcccgat 
      gctgtggtc gacgagaga ttgagagtt tatgggatt 
      aaagggtta agggtggtc gatgggtcc atcatgccc 
      aatgtggtg gccggacat accaatgct gtgataatg 
      atgattggg gagaaggct tcagacatg atcaagcaa 
      gagtgggca agaaagtga
    mRNA sequence
    • >TC034017 RA
      cacggttga aaatgccgc gaaaatgaa gacaatcct 
      gtgttttac tgtgctttt cttgtgtgc aaactccca 
      aaacctcct caacgactt ccgccgctt tttcggggc 
      gagtgacgt ccgcgacac cgcgaattt tcgcaaaag 
      ctacgactt tgtggtcgt gggggcggg ctccggggg 
      ctgcgtcgt cgccaaccg tctgagcga aaaccccga 
      gtggtccgt cctgttact ggaagccgg cgatgacga 
      aaatttttt gaccgatgt cccgctaat tgcctcgct 
      tcaaaccat cacgagcta caattgggg ctataagag 
      cgaaagact cgcgacggc ctgtttggg gctcatcga 
      cggccgctg taacatgcc gaggggcaa agccctggg 
      gggcacctc cgtgatcaa ttttttgct atacaccag 
      gggcaccaa gcaggactt tgaccaatg ggctgagtt 
      gggaaaccc aggctgggg gtacgacca agtcctccc 
      ctatttcat caaatcgga aaattgcac caaatgccg 
      ggaaattga cgggaaata tcacgggaa aagcggcta 
      cttgagtgt ggaacaccc ggggtatga gtcacccct 
      agtcaagcg tttcataaa atccgggga agaactggg 
      ttacaagaa caacgaccc tagcgcccc ctatggact 
      gggtttttc caaagtttt ggcaacaat gaggaacgg 
      gatgcggtg cagtgcctc taaggcgtt cctcaagcc 
      cattttaca ccgcacaaa cttacacgt gtcgattaa 
      aacacgtgt caccaaaat cctaatcga tccgagcac 
      caaacaagc atacggcgt ccaattttg gaaaaatcg 
      ccgaaaatt taccgtcct agccaccaa agaagtggt 
      tttgtcggc cggttcgat caacagccc ccatttact 
      gatgctgtc gggcgtggg cccccggga cgatttaac 
      ccgtgtggg aatccccct cttgcaaaa tttaaaagt 
      cgggtataa cctacagga ccatatggc gatgtccgc 
      actggtgtt tttcgtcaa tgagtcaat cactgtgtc 
      ggaccgtgg ggtgcaaaa ccccgttga tatttttaa 
      ttatgtgtt taatggaag ggggcccta tactatacc 
      tgggggggc cgaagccct ggctttcgt gcagacgaa 
      gtatgcaaa aatcggggg ttaccccga tattgaatt 
      ggtgttagg tgccggggc gctgaatgg cgatgtgta 
      tggaagttt gaggagtct tttggggat tccccgaag 
      tttgttcga gagggttta cgccccaca tgcgtacaa 
      accggcgtt tagtatcgc accggtttt gatgaggcc 
      caaaagtcg aggcagggt tgtgatcaa agatgggaa 
      tcccctgca ttggcctaa attaatacc gaattattt 
      cgagaatga ggaggatgt caaaacaat ggtcgaggg 
      gatcaagat ggcaaggtg gtccagctc agcttccgt 
      cttctgtag ctcagcgat tacaattac gcaatctcg 
      acattttca aaaatacaa cattacaat gattacgac 
      cccatttcc tgggtgtga aactgtacc atttggtag 
      cgacgagta ttgggcttg tgccgttcg gcacgttgc 
      caccactct ggggcacca ggttgggac ctgcaaaat 
      ggggccccc gagcgatcc cgatgctgt ggtcgacga 
      gagattgag agtttatgg gattaaagg gttaagggt 
      ggtcgatgg gtccatcat gcccaatgt ggtggccgg 
      acataccaa tgctgtgat aatgatgat tggggagaa 
      ggcttcaga catgatcaa gcaagagtg ggcaagaaa 
      gtgactaga accggtttt tggtgcata aagtacggg 
      tttgttgat tgtataaga ctgtaatta aataaaagc 
      gattttcta tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY