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Gene information
General information
TC005735 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8350425..8356392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005735
      iBeetle screen iB_06201

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005735 RA
        MLIELIRKW LKKRLGIHL SIIPKYFYD ATRDGTLVA 
        QILLYYDVI TPEEANLIE ETQDPAQAF DNYQHHVAK 
        WLSRIKLHL TESEMYEIA YCEGTVAAT IFYELYLKL 
        EAKEDSSKI RPKNHHFRI EKVTEFQID DVFIEANPL 
        CEPLIKQWD IINWEKDHL ASLMMKYMN SREEYRNYL 
        EEKKIEELS APANLEKLE DTLIFLKAE KTDYTYDQL 
        IAQQKAALE MEPFQPNQD EAKRIMAKI KRRKKQRAQ 
        RTAFRLELQ HQLLSDVWK KIEESQELE FDKVICEKL 
        LKQSFHEKE IEEKLLFIR KQKDTIVAN RRVQDEIDQ 
        KKRDEEFIR RLFEEDLEE RLRFHDERM ALIAEHKRL 
        YEEKMRLKA LKHLKLCQE VMGDIVDLS IKTAEYKEM 
        YNMEPNKYV QRQWANLFI KQQPIFDLV VPSQDLKQP 
        HEEISAELE QIYRREIQR QDILDEQDF DDYINFRNI 
        WQVEEGLEE YLAAMELGL NCLGFAVHK LLLKKYPLP 
        VPEKAQVSP EIEIAVCAT LADLTRIDM LQRLLNLRN 
        IKVILIEDA VNFCIEGYE SELKTEFDE PFPEEEYLR 
        QLTPKKGKK EEKKKKKGK KGDKKSKKK GDKASKKKS 
        DKSSKKSKK GKKKGGEKT PVEVTFEEK QAQTPRLFP 
        WEKVVFSNR GKLGRLAKQ LLGQGDPLD DKLQVDMFI 
        EYLLSLKDI KGWVLLNFP NTYEQAALL EEALSGKAA 
        PKQYFDKSI NKESLTSVE VEQVEVEGE EISSSTTAD 
        QVTPEEIPP EESVKRSEE KPRSRVNIA EYLNQSLPS 
        FASTDTGCI SEVTMQTDF SYIPEVRVK RLLPDLINL 
        AYSSEEENE PETEPDPKK CICVCNQDN EDGNFGEEV 
        EVEMNEDLS EEKKSVEYD EDPDEKKSE QNQDCVCYV 
        DKTEEVVPV EVQEATIQT DILGLMHEL GASDSKPIE 
        RIVVEDIYE EKRLSKIVP NPRPLEKEE YNTVLTAFI 
        KVRQKEEAE DEDFPEPYF KEMERKDGL EKFYCDQGC 
        YYILQYDEF NVETIKCLA KMIIGDYNL PLKDSIEIF 
        GEHYKEALL DVGGHQIVK KGKKEKKKK GKEGKKAKP 
        SKSKSTTKS GKKNKKSEK SSKKSKKSK KSDKSSKKS 
        KGKKGKKGK KAKSTHDLL APVAHEDKE VQFPAVEEL 
        EEEELPEPR AGEPDWEYT NLAIPPEIE LILANHWDN 
        FEENYIKNF KDLFFNKRL LLEEIVPFL ARTRETMGE 
        FIVRPDQKQ YYLREFQKT LNEIGLDLR NDQETKTEL 
        LCRIEEFRD KLLGICDTK MQESEEERR RFVRQNWLF 
        HQFCQITNN SAEAFQFEI DRIVDTLQF LSDYYVSVI 
        TKSSKEGYD FHKQILPKF ECDDSQSDD FNSLFIDVE 
        SENCEKSVF HEYITVSNQ GALGFVDSM KLLALKPVH 
        DMRKLFEAG EKGKEKKKK KPKAKQKKF ELEEEVMES 
        QERIFEEWQ CAIEGELAR AYVHLSVIR NEAFNRIND 
        AISWIKRIF EEIYDTIGE TYEISLSNV NKACELLAL 
        SVEQEMIIQ PELAFEGEQ LAQIQVALF PDTLAVAEE 
        GYSEGYFKI EQLEFMVKV LLDLAHSGY IMKNCFLLL 
        IQDILYYDF DDSEDVETP KLWRNLPIS ALNTLLRKL 
        FDDEKYLYW RDFIICNLM VPLPTREQL MKVRQQFRQ 
        YDPDLTEKV TKDAFESIE FWFEDKRVK SLLFSMYQL 
        DENEVNYTA LLLDFCKDN DPIIGFVKA LELSLGQFI 
        CCDKAVGEK FVAEVLEDR ILFEKRKKE HEENIILAK 
        RAVSLLIDR IVGQFEEPE GFSLKEVED TIEERSLSR 
        SFQSDFVGQ IITEHKFSF TNIGFEARD DTGMAFFLP 
        QGLVQIIVN TDITWNSKI ELPNSESFS DKIMNIYDS 
        CRNEKFNNV VLAHEFLNC DELRELFQN TNKFSAHNP 
        VKLVKRIVT EAELSEPEE FVFHQESKH SLNTDTTKT 
        HSSY
      CDS sequence
      • >TC005735 RA
        atgctgatt gaattgatc aggaaatgg ctgaaaaaa 
        cgcctaggc atccatttg agcatcatt cccaagtac 
        ttctacgac gcgacgcgg gacggtacc ctcgtggct 
        caaatcctc ctctactac gacgtcata actcctgaa 
        gaagcgaac ctaattgaa gaaacgcaa gacccggcc 
        caagccttc gataactac caacaccac gtggctaaa 
        tggttgagt cgcatcaaa ctgcacctc accgaaagt 
        gaaatgtac gaaatcgcc tactgtgaa ggcacagtc 
        gctgccaca atcttctac gagttgtac ttaaaactc 
        gaagccaag gaggactcc agtaaaatt aggcccaag 
        aatcaccat ttccgcatc gagaaagtc accgagttt 
        caaatcgac gatgttttt atcgaggct aatcccttg 
        tgcgagcct ttgatcaaa caatgggac atcatcaac 
        tgggaaaag gaccacctg gcgagtctc atgatgaaa 
        tacatgaac tcgcgggaa gaataccgc aattatttg 
        gaagaaaag aagattgag gaattgagt gcgccggca 
        aatttggaa aaactggaa gacactctt atttttctg 
        aaggcggaa aagacggat tatacctac gaccagttg 
        atagcccag caaaaggca gcccttgaa atggaaccc 
        ttccagcca aaccaagac gaagccaaa cgaatcatg 
        gccaaaatt aagcgacga aaaaagcaa agggcccaa 
        agaaccgca ttcagactc gaactgcaa caccaactc 
        ctttcggac gtttggaag aaaatcgaa gagagtcaa 
        gaactcgaa tttgataaa gtcatttgc gaaaaactg 
        ttaaaacag tcgtttcac gagaaggaa atcgaagaa 
        aagctcctc tttatacgg aaacagaaa gacactatt 
        gtggcgaac cgtagagtc caggacgaa atcgatcag 
        aagaaacgg gacgaggaa ttcattcgg agactcttc 
        gaggaagat ctcgaagaa cgcttgcgg tttcatgac 
        gagaggatg gccctcata gctgagcat aagcgcctc 
        tacgaggag aaaatgcga ctcaaggcg ctcaagcac 
        ctgaaactg tgccaggaa gtgatgggt gatatcgtc 
        gacctttcc ataaaaaca gcagagtac aaggagatg 
        tacaacatg gagcccaac aaatacgtg caaagacaa 
        tgggcgaat cttttcata aagcaacaa ccgattttt 
        gacttggtt gtgccttcg caagacttg aaacagcca 
        cacgaggag atttcggcg gaactggag caaatttac 
        aggagggag attcagaga caggacatc ctggacgag 
        caagatttt gacgattac atcaacttt cgcaacatt 
        tggcaagtt gaggaggga cttgaggaa tatctcgct 
        gcgatggag ctcggactt aattgtttg ggcttcgcc 
        gtccacaaa ctcctcctc aaaaagtac cccctgcca 
        gtaccggag aaagcccaa gtgtctccg gaaatcgag 
        attgcggtt tgtgccact ttggccgac ttgacccgg 
        attgacatg ctccagcgt ctcctcaac ctcagaaat 
        atcaaagtg attttgatc gaagacgcg gttaatttt 
        tgcatcgaa ggctacgaa agcgaactg aagacggag 
        tttgatgag ccgttccct gaggaagag tacttgcgc 
        cagctgacg cccaaaaaa ggcaaaaaa gaggagaag 
        aagaagaaa aagggcaaa aaaggggac aaaaaatca 
        aagaaaaaa ggggacaaa gcctcgaag aaaaaaagc 
        gacaaatcc tcgaagaaa agcaagaag gggaagaaa 
        aaagggggg gagaagaca cccgtggag gtgacgttt 
        gaggagaag caagcccaa accccgcgc ctgttcccg 
        tgggagaaa gtggttttc tcaaacagg ggcaagttg 
        ggccgtttg gccaaacaa ctgctaggt cagggtgac 
        cctcttgat gataagctc caagttgat atgtttatc 
        gagtatttg ttgtcactc aaggatatc aaaggctgg 
        gttttgctc aacttcccc aacacttac gaacaagcg 
        gccctcctt gaggaggct ctgagtggc aaagccgcc 
        ccgaaacaa tactttgac aagtccata aacaaagag 
        agtctcacc agtgttgaa gttgaacaa gttgaagtt 
        gaaggggag gaaatttcc agtagtacc accgcggac 
        caagtcaca ccggaggaa atccctccg gaggaaagt 
        gtgaagaga agtgaggaa aaaccacgt agtagagtg 
        aacattgct gaatatttg aaccagtcg cttccatct 
        ttcgccagt acggataca gggtgtata agcgaggtc 
        acaatgcag accgatttc agttacatt ccggaagtc 
        agagtgaag agactactc cccgatttg atcaacttg 
        gcgtattcc tcggaggag gaaaacgag cctgagact 
        gaaccagac cccaaaaag tgcatctgt gtgtgcaac 
        caagacaat gaagatggt aattttggt gaagaagtt 
        gaagttgaa atgaacgaa gatctatca gaagagaaa 
        aaatcagtc gagtatgat gaggatccc gacgagaag 
        aagagtgaa caaaatcag gactgtgtt tgctatgtg 
        gataaaaca gaagaggtg gttccggtt gaggtccag 
        gaagccaca atccagact gacattttg ggtttaatg 
        cacgagctt ggagcgagt gactctaaa ccgatcgag 
        cgaattgtt gttgaagat atttacgaa gaaaagcgc 
        ttgtcgaag attgttcca aacccgcga cccttggaa 
        aaggaagaa tataacacc gtacttacc gcctttata 
        aaagtcagg cagaaggag gaggccgaa gacgaggat 
        ttcccggag ccttatttc aaagaaatg gaacggaag 
        gatggatta gagaagttt tactgtgac caagggtgt 
        tactacatt ttacagtac gacgagttt aatgttgaa 
        actatcaaa tgcttggcc aagatgatc atcggtgac 
        tataacttg cccttgaaa gactcaatt gagattttt 
        ggggagcac tacaaggag gcactgctt gatgttggc 
        ggccaccaa atcgtaaag aaagggaag aaggagaag 
        aaaaaaaag ggcaaggag gggaaaaag gccaaaccc 
        agcaaaagc aaaagcacg acaaagtca ggaaaaaag 
        aacaaaaag tctgaaaag tcctccaaa aagagcaag 
        aaaagcaaa aagtcggat aaatcctcc aaaaaaagc 
        aagggcaaa aagggtaaa aaaggcaag aaggccaaa 
        agcacgcac gacctcttg gcaccagtg gcccacgaa 
        gacaaagag gtgcaattc ccggcggtt gaggagctc 
        gaggaggag gaactgccc gaaccccgg gctggggag 
        cccgactgg gagtacacg aacctggca atcccgccc 
        gagattgag ctaatccta gccaaccac tgggacaac 
        ttcgaggag aactacatc aaaaacttc aaggacttg 
        ttcttcaac aagcggctg ttgctggag gaaattgtg 
        ccgttcctg gcccgcact cgggagacc atgggcgag 
        tttatagtc cgccctgac caaaaacaa tactacttg 
        agggaattc caaaagacg ctcaacgaa atcggtcta 
        gacttgagg aacgaccaa gagaccaaa accgagctt 
        ttgtgccgg attgaggaa ttccgcgac aaactcctg 
        gggatttgt gacaccaaa atgcaggag tcggaggag 
        gagaggagg cggttcgtg aggcaaaac tggctcttt 
        caccagttt tgccaaatc acgaataac agcgctgag 
        gcgttccag tttgaaatc gaccgaatc gttgacacg 
        ttgcagttc ttgtccgat tattacgtt tccgtgatt 
        acgaaatcc tcaaaggag ggctacgac ttccataag 
        cagatcctg ccgaagttt gagtgtgac gattcgcaa 
        agtgacgac ttcaactcg ctttttatc gacgtggag 
        agcgaaaat tgcgagaaa agtgtgttt catgaatat 
        atcactgtt agtaaccag ggggctttg ggtttcgtc 
        gatagcatg aaactgcta gccttgaag ccggtccac 
        gacatgagg aagttgttt gaagcgggg gagaagggc 
        aaggagaag aagaagaag aaaccaaaa gcgaagcag 
        aaaaaattc gagttggag gaggaggtg atggagagc 
        caggagcgg atttttgag gagtggcaa tgcgcgatt 
        gagggcgaa ctcgcgcgg gcgtacgtc cacttgagt 
        gtcatccgg aacgaggcg ttcaatcgc atcaatgac 
        gcgattagt tggatcaag cgaattttt gaggagatt 
        tacgatact ataggcgaa acgtacgag attagtctc 
        tcgaatgtg aacaaggcg tgcgaattg ttggcgctg 
        tccgtggaa caggagatg ataattcaa ccggagctg 
        gcgttcgaa ggggaacag ttggcccaa atccaggtc 
        gctttgttc cccgacact ttggcggtg gctgaggag 
        ggttacagt gaggggtat ttcaaaatc gagcagctg 
        gagttcatg gtcaaagtt ctcctcgat ttggcacac 
        agtgggtac ataatgaag aactgcttc ctgctgctg 
        atccaggac atcctctac tacgacttt gacgattct 
        gaagacgtt gaaacgccg aaactgtgg cgaaacttg 
        ccaatttcc gccctcaat accttgctg cgtaagctg 
        ttcgatgac gagaagtat ttatattgg agggacttt 
        attatttgt aatttaatg gtcccgctc cctacccgg 
        gaacagttg atgaaagtg cggcaacag ttccggcaa 
        tacgacccg gacttaacc gaaaaagtc accaaggat 
        gcgtttgag tcgattgag ttctggttt gaggacaag 
        cgtgtcaaa agcttgctc ttttcaatg taccaactc 
        gacgaaaat gaagttaac tacactgca cttctgctc 
        gacttttgc aaagataac gacccgata attgggttt 
        gtcaaggct ctggagctg tctctgggc caatttatt 
        tgttgcgac aaagccgtc ggagagaaa ttcgtggcg 
        gaggttctg gaagaccgg attttattc gagaagagg 
        aaaaaagag cacgaggaa aacataatt ctggctaaa 
        agggccgtg agtctccta attgaccga attgtcggt 
        cagtttgag gagcccgaa ggttttagt ttgaaggag 
        gtagaggac acgattgag gaacggtcg ctttcgcgg 
        agtttccag tcggacttt gtcggtcaa ataatcaca 
        gagcacaag ttttccttt acgaatatc gggttcgag 
        gcccgggat gacacagga atggcgttc ttcctgccg 
        cagggcctg gtccagata attgtcaat accgacata 
        acgtggaat tccaaaatc gagctcccc aactcggag 
        agtttcagc gacaaaatc atgaacatt tacgacagt 
        tgccggaac gagaagttc aacaatgtg gtgttagcg 
        cacgagttt ctcaactgt gacgaattg agggaattg 
        ttccaaaac actaataaa ttctcagct cataatccc 
        gtcaaactt gtcaaacgc atagtcact gaagcggaa 
        ctaagcgaa ccggaagag ttcgtcttt caccaggag 
        tcgaagcat tcgctcaac accgacaca acgaaaacg 
        cacagttct tattaa
      mRNA sequence
      • >TC005735 RA
        aggaagtaa ataaatgag attcaatta acattttgt 
        gaaataaat cgacgtaac cccactttc gtggcgcca 
        aatttgaat gacaacttg tcgcaaaaa tgctgattg 
        aattgatca ggaaatggc tgaaaaaac gcctaggca 
        tccatttga gcatcattc ccaagtact tctacgacg 
        cgacgcggg acggtaccc tcgtggctc aaatcctcc 
        tctactacg acgtcataa ctcctgaag aagcgaacc 
        taattgaag aaacgcaag acccggccc aagccttcg 
        ataactacc aacaccacg tggctaaat ggttgagtc 
        gcatcaaac tgcacctca ccgaaagtg aaatgtacg 
        aaatcgcct actgtgaag gcacagtcg ctgccacaa 
        tcttctacg agttgtact taaaactcg aagccaagg 
        aggactcca gtaaaatta ggcccaaga atcaccatt 
        tccgcatcg agaaagtca ccgagtttc aaatcgacg 
        atgttttta tcgaggcta atcccttgt gcgagcctt 
        tgatcaaac aatgggaca tcatcaact gggaaaagg 
        accacctgg cgagtctca tgatgaaat acatgaact 
        cgcgggaag aataccgca attatttgg aagaaaaga 
        agattgagg aattgagtg cgccggcaa atttggaaa 
        aactggaag acactctta tttttctga aggcggaaa 
        agacggatt atacctacg accagttga tagcccagc 
        aaaaggcag cccttgaaa tggaaccct tccagccaa 
        accaagacg aagccaaac gaatcatgg ccaaaatta 
        agcgacgaa aaaagcaaa gggcccaaa gaaccgcat 
        tcagactcg aactgcaac accaactcc tttcggacg 
        tttggaaga aaatcgaag agagtcaag aactcgaat 
        ttgataaag tcatttgcg aaaaactgt taaaacagt 
        cgtttcacg agaaggaaa tcgaagaaa agctcctct 
        ttatacgga aacagaaag acactattg tggcgaacc 
        gtagagtcc aggacgaaa tcgatcaga agaaacggg 
        acgaggaat tcattcgga gactcttcg aggaagatc 
        tcgaagaac gcttgcggt ttcatgacg agaggatgg 
        ccctcatag ctgagcata agcgcctct acgaggaga 
        aaatgcgac tcaaggcgc tcaagcacc tgaaactgt 
        gccaggaag tgatgggtg atatcgtcg acctttcca 
        taaaaacag cagagtaca aggagatgt acaacatgg 
        agcccaaca aatacgtgc aaagacaat gggcgaatc 
        ttttcataa agcaacaac cgatttttg acttggttg 
        tgccttcgc aagacttga aacagccac acgaggaga 
        tttcggcgg aactggagc aaatttaca ggagggaga 
        ttcagagac aggacatcc tggacgagc aagattttg 
        acgattaca tcaactttc gcaacattt ggcaagttg 
        aggagggac ttgaggaat atctcgctg cgatggagc 
        tcggactta attgtttgg gcttcgccg tccacaaac 
        tcctcctca aaaagtacc ccctgccag taccggaga 
        aagcccaag tgtctccgg aaatcgaga ttgcggttt 
        gtgccactt tggccgact tgacccgga ttgacatgc 
        tccagcgtc tcctcaacc tcagaaata tcaaagtga 
        ttttgatcg aagacgcgg ttaattttt gcatcgaag 
        gctacgaaa gcgaactga agacggagt ttgatgagc 
        cgttccctg aggaagagt acttgcgcc agctgacgc 
        ccaaaaaag gcaaaaaag aggagaaga agaagaaaa 
        agggcaaaa aaggggaca aaaaatcaa agaaaaaag 
        gggacaaag cctcgaaga aaaaaagcg acaaatcct 
        cgaagaaaa gcaagaagg ggaagaaaa aaggggggg 
        agaagacac ccgtggagg tgacgtttg aggagaagc 
        aagcccaaa ccccgcgcc tgttcccgt gggagaaag 
        tggttttct caaacaggg gcaagttgg gccgtttgg 
        ccaaacaac tgctaggtc agggtgacc ctcttgatg 
        ataagctcc aagttgata tgtttatcg agtatttgt 
        tgtcactca aggatatca aaggctggg ttttgctca 
        acttcccca acacttacg aacaagcgg ccctccttg 
        aggaggctc tgagtggca aagccgccc cgaaacaat 
        actttgaca agtccataa acaaagaga gtctcacca 
        gtgttgaag ttgaacaag ttgaagttg aaggggagg 
        aaatttcca gtagtacca ccgcggacc aagtcacac 
        cggaggaaa tccctccgg aggaaagtg tgaagagaa 
        gtgaggaaa aaccacgta gtagagtga acattgctg 
        aatatttga accagtcgc ttccatctt tcgccagta 
        cggatacag ggtgtataa gcgaggtca caatgcaga 
        ccgatttca gttacattc cggaagtca gagtgaaga 
        gactactcc ccgatttga tcaacttgg cgtattcct 
        cggaggagg aaaacgagc ctgagactg aaccagacc 
        ccaaaaagt gcatctgtg tgtgcaacc aagacaatg 
        aagatggta attttggtg aagaagttg aagttgaaa 
        tgaacgaag atctatcag aagagaaaa aatcagtcg 
        agtatgatg aggatcccg acgagaaga agagtgaac 
        aaaatcagg actgtgttt gctatgtgg ataaaacag 
        aagaggtgg ttccggttg aggtccagg aagccacaa 
        tccagactg acattttgg gtttaatgc acgagcttg 
        gagcgagtg actctaaac cgatcgagc gaattgttg 
        ttgaagata tttacgaag aaaagcgct tgtcgaaga 
        ttgttccaa acccgcgac ccttggaaa aggaagaat 
        ataacaccg tacttaccg cctttataa aagtcaggc 
        agaaggagg aggccgaag acgaggatt tcccggagc 
        cttatttca aagaaatgg aacggaagg atggattag 
        agaagtttt actgtgacc aagggtgtt actacattt 
        tacagtacg acgagttta atgttgaaa ctatcaaat 
        gcttggcca agatgatca tcggtgact ataacttgc 
        ccttgaaag actcaattg agatttttg gggagcact 
        acaaggagg cactgcttg atgttggcg gccaccaaa 
        tcgtaaaga aagggaaga aggagaaga aaaaaaagg 
        gcaaggagg ggaaaaagg ccaaaccca gcaaaagca 
        aaagcacga caaagtcag gaaaaaaga acaaaaagt 
        ctgaaaagt cctccaaaa agagcaaga aaagcaaaa 
        agtcggata aatcctcca aaaaaagca agggcaaaa 
        agggtaaaa aaggcaaga aggccaaaa gcacgcacg 
        acctcttgg caccagtgg cccacgaag acaaagagg 
        tgcaattcc cggcggttg aggagctcg aggaggagg 
        aactgcccg aaccccggg ctggggagc ccgactggg 
        agtacacga acctggcaa tcccgcccg agattgagc 
        taatcctag ccaaccact gggacaact tcgaggaga 
        actacatca aaaacttca aggacttgt tcttcaaca 
        agcggctgt tgctggagg aaattgtgc cgttcctgg 
        cccgcactc gggagacca tgggcgagt ttatagtcc 
        gccctgacc aaaaacaat actacttga gggaattcc 
        aaaagacgc tcaacgaaa tcggtctag acttgagga 
        acgaccaag agaccaaaa ccgagcttt tgtgccgga 
        ttgaggaat tccgcgaca aactcctgg ggatttgtg 
        acaccaaaa tgcaggagt cggaggagg agaggaggc 
        ggttcgtga ggcaaaact ggctctttc accagtttt 
        gccaaatca cgaataaca gcgctgagg cgttccagt 
        ttgaaatcg accgaatcg ttgacacgt tgcagttct 
        tgtccgatt attacgttt ccgtgatta cgaaatcct 
        caaaggagg gctacgact tccataagc agatcctgc 
        cgaagtttg agtgtgacg attcgcaaa gtgacgact 
        tcaactcgc tttttatcg acgtggaga gcgaaaatt 
        gcgagaaaa gtgtgtttc atgaatata tcactgtta 
        gtaaccagg gggctttgg gtttcgtcg atagcatga 
        aactgctag ccttgaagc cggtccacg acatgagga 
        agttgtttg aagcggggg agaagggca aggagaaga 
        agaagaaga aaccaaaag cgaagcaga aaaaattcg 
        agttggagg aggaggtga tggagagcc aggagcgga 
        tttttgagg agtggcaat gcgcgattg agggcgaac 
        tcgcgcggg cgtacgtcc acttgagtg tcatccgga 
        acgaggcgt tcaatcgca tcaatgacg cgattagtt 
        ggatcaagc gaatttttg aggagattt acgatacta 
        taggcgaaa cgtacgaga ttagtctct cgaatgtga 
        acaaggcgt gcgaattgt tggcgctgt ccgtggaac 
        aggagatga taattcaac cggagctgg cgttcgaag 
        gggaacagt tggcccaaa tccaggtcg ctttgttcc 
        ccgacactt tggcggtgg ctgaggagg gttacagtg 
        aggggtatt tcaaaatcg agcagctgg agttcatgg 
        tcaaagttc tcctcgatt tggcacaca gtgggtaca 
        taatgaaga actgcttcc tgctgctga tccaggaca 
        tcctctact acgactttg acgattctg aagacgttg 
        aaacgccga aactgtggc gaaacttgc caatttccg 
        ccctcaata ccttgctgc gtaagctgt tcgatgacg 
        agaagtatt tatattgga gggacttta ttatttgta 
        atttaatgg tcccgctcc ctacccggg aacagttga 
        tgaaagtgc ggcaacagt tccggcaat acgacccgg 
        acttaaccg aaaaagtca ccaaggatg cgtttgagt 
        cgattgagt tctggtttg aggacaagc gtgtcaaaa 
        gcttgctct tttcaatgt accaactcg acgaaaatg 
        aagttaact acactgcac ttctgctcg acttttgca 
        aagataacg acccgataa ttgggtttg tcaaggctc 
        tggagctgt ctctgggcc aatttattt gttgcgaca 
        aagccgtcg gagagaaat tcgtggcgg aggttctgg 
        aagaccgga ttttattcg agaagagga aaaaagagc 
        acgaggaaa acataattc tggctaaaa gggccgtga 
        gtctcctaa ttgaccgaa ttgtcggtc agtttgagg 
        agcccgaag gttttagtt tgaaggagg tagaggaca 
        cgattgagg aacggtcgc tttcgcgga gtttccagt 
        cggactttg tcggtcaaa taatcacag agcacaagt 
        tttccttta cgaatatcg ggttcgagg cccgggatg 
        acacaggaa tggcgttct tcctgccgc agggcctgg 
        tccagataa ttgtcaata ccgacataa cgtggaatt 
        ccaaaatcg agctcccca actcggaga gtttcagcg 
        acaaaatca tgaacattt acgacagtt gccggaacg 
        agaagttca acaatgtgg tgttagcgc acgagtttc 
        tcaactgtg acgaattga gggaattgt tccaaaaca 
        ctaataaat tctcagctc ataatcccg tcaaacttg 
        tcaaacgca tagtcactg aagcggaac taagcgaac 
        cggaagagt tcgtctttc accaggagt cgaagcatt 
        cgctcaaca ccgacacaa cgaaaacgc acagttctt 
        attaaaaat ctttatttt tctcatata t
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