| General information | |||
| TC005735 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 8350425..8356392 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005735 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_06201 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC005735 RA
MLIELIRKW LKKRLGIHL SIIPKYFYD ATRDGTLVA QILLYYDVI TPEEANLIE ETQDPAQAF DNYQHHVAK WLSRIKLHL TESEMYEIA YCEGTVAAT IFYELYLKL EAKEDSSKI RPKNHHFRI EKVTEFQID DVFIEANPL CEPLIKQWD IINWEKDHL ASLMMKYMN SREEYRNYL EEKKIEELS APANLEKLE DTLIFLKAE KTDYTYDQL IAQQKAALE MEPFQPNQD EAKRIMAKI KRRKKQRAQ RTAFRLELQ HQLLSDVWK KIEESQELE FDKVICEKL LKQSFHEKE IEEKLLFIR KQKDTIVAN RRVQDEIDQ KKRDEEFIR RLFEEDLEE RLRFHDERM ALIAEHKRL YEEKMRLKA LKHLKLCQE VMGDIVDLS IKTAEYKEM YNMEPNKYV QRQWANLFI KQQPIFDLV VPSQDLKQP HEEISAELE QIYRREIQR QDILDEQDF DDYINFRNI WQVEEGLEE YLAAMELGL NCLGFAVHK LLLKKYPLP VPEKAQVSP EIEIAVCAT LADLTRIDM LQRLLNLRN IKVILIEDA VNFCIEGYE SELKTEFDE PFPEEEYLR QLTPKKGKK EEKKKKKGK KGDKKSKKK GDKASKKKS DKSSKKSKK GKKKGGEKT PVEVTFEEK QAQTPRLFP WEKVVFSNR GKLGRLAKQ LLGQGDPLD DKLQVDMFI EYLLSLKDI KGWVLLNFP NTYEQAALL EEALSGKAA PKQYFDKSI NKESLTSVE VEQVEVEGE EISSSTTAD QVTPEEIPP EESVKRSEE KPRSRVNIA EYLNQSLPS FASTDTGCI SEVTMQTDF SYIPEVRVK RLLPDLINL AYSSEEENE PETEPDPKK CICVCNQDN EDGNFGEEV EVEMNEDLS EEKKSVEYD EDPDEKKSE QNQDCVCYV DKTEEVVPV EVQEATIQT DILGLMHEL GASDSKPIE RIVVEDIYE EKRLSKIVP NPRPLEKEE YNTVLTAFI KVRQKEEAE DEDFPEPYF KEMERKDGL EKFYCDQGC YYILQYDEF NVETIKCLA KMIIGDYNL PLKDSIEIF GEHYKEALL DVGGHQIVK KGKKEKKKK GKEGKKAKP SKSKSTTKS GKKNKKSEK SSKKSKKSK KSDKSSKKS KGKKGKKGK KAKSTHDLL APVAHEDKE VQFPAVEEL EEEELPEPR AGEPDWEYT NLAIPPEIE LILANHWDN FEENYIKNF KDLFFNKRL LLEEIVPFL ARTRETMGE FIVRPDQKQ YYLREFQKT LNEIGLDLR NDQETKTEL LCRIEEFRD KLLGICDTK MQESEEERR RFVRQNWLF HQFCQITNN SAEAFQFEI DRIVDTLQF LSDYYVSVI TKSSKEGYD FHKQILPKF ECDDSQSDD FNSLFIDVE SENCEKSVF HEYITVSNQ GALGFVDSM KLLALKPVH DMRKLFEAG EKGKEKKKK KPKAKQKKF ELEEEVMES QERIFEEWQ CAIEGELAR AYVHLSVIR NEAFNRIND AISWIKRIF EEIYDTIGE TYEISLSNV NKACELLAL SVEQEMIIQ PELAFEGEQ LAQIQVALF PDTLAVAEE GYSEGYFKI EQLEFMVKV LLDLAHSGY IMKNCFLLL IQDILYYDF DDSEDVETP KLWRNLPIS ALNTLLRKL FDDEKYLYW RDFIICNLM VPLPTREQL MKVRQQFRQ YDPDLTEKV TKDAFESIE FWFEDKRVK SLLFSMYQL DENEVNYTA LLLDFCKDN DPIIGFVKA LELSLGQFI CCDKAVGEK FVAEVLEDR ILFEKRKKE HEENIILAK RAVSLLIDR IVGQFEEPE GFSLKEVED TIEERSLSR SFQSDFVGQ IITEHKFSF TNIGFEARD DTGMAFFLP QGLVQIIVN TDITWNSKI ELPNSESFS DKIMNIYDS CRNEKFNNV VLAHEFLNC DELRELFQN TNKFSAHNP VKLVKRIVT EAELSEPEE FVFHQESKH SLNTDTTKT HSSY
- >TC005735 RA
atgctgatt gaattgatc aggaaatgg ctgaaaaaa cgcctaggc atccatttg agcatcatt cccaagtac ttctacgac gcgacgcgg gacggtacc ctcgtggct caaatcctc ctctactac gacgtcata actcctgaa gaagcgaac ctaattgaa gaaacgcaa gacccggcc caagccttc gataactac caacaccac gtggctaaa tggttgagt cgcatcaaa ctgcacctc accgaaagt gaaatgtac gaaatcgcc tactgtgaa ggcacagtc gctgccaca atcttctac gagttgtac ttaaaactc gaagccaag gaggactcc agtaaaatt aggcccaag aatcaccat ttccgcatc gagaaagtc accgagttt caaatcgac gatgttttt atcgaggct aatcccttg tgcgagcct ttgatcaaa caatgggac atcatcaac tgggaaaag gaccacctg gcgagtctc atgatgaaa tacatgaac tcgcgggaa gaataccgc aattatttg gaagaaaag aagattgag gaattgagt gcgccggca aatttggaa aaactggaa gacactctt atttttctg aaggcggaa aagacggat tatacctac gaccagttg atagcccag caaaaggca gcccttgaa atggaaccc ttccagcca aaccaagac gaagccaaa cgaatcatg gccaaaatt aagcgacga aaaaagcaa agggcccaa agaaccgca ttcagactc gaactgcaa caccaactc ctttcggac gtttggaag aaaatcgaa gagagtcaa gaactcgaa tttgataaa gtcatttgc gaaaaactg ttaaaacag tcgtttcac gagaaggaa atcgaagaa aagctcctc tttatacgg aaacagaaa gacactatt gtggcgaac cgtagagtc caggacgaa atcgatcag aagaaacgg gacgaggaa ttcattcgg agactcttc gaggaagat ctcgaagaa cgcttgcgg tttcatgac gagaggatg gccctcata gctgagcat aagcgcctc tacgaggag aaaatgcga ctcaaggcg ctcaagcac ctgaaactg tgccaggaa gtgatgggt gatatcgtc gacctttcc ataaaaaca gcagagtac aaggagatg tacaacatg gagcccaac aaatacgtg caaagacaa tgggcgaat cttttcata aagcaacaa ccgattttt gacttggtt gtgccttcg caagacttg aaacagcca cacgaggag atttcggcg gaactggag caaatttac aggagggag attcagaga caggacatc ctggacgag caagatttt gacgattac atcaacttt cgcaacatt tggcaagtt gaggaggga cttgaggaa tatctcgct gcgatggag ctcggactt aattgtttg ggcttcgcc gtccacaaa ctcctcctc aaaaagtac cccctgcca gtaccggag aaagcccaa gtgtctccg gaaatcgag attgcggtt tgtgccact ttggccgac ttgacccgg attgacatg ctccagcgt ctcctcaac ctcagaaat atcaaagtg attttgatc gaagacgcg gttaatttt tgcatcgaa ggctacgaa agcgaactg aagacggag tttgatgag ccgttccct gaggaagag tacttgcgc cagctgacg cccaaaaaa ggcaaaaaa gaggagaag aagaagaaa aagggcaaa aaaggggac aaaaaatca aagaaaaaa ggggacaaa gcctcgaag aaaaaaagc gacaaatcc tcgaagaaa agcaagaag gggaagaaa aaagggggg gagaagaca cccgtggag gtgacgttt gaggagaag caagcccaa accccgcgc ctgttcccg tgggagaaa gtggttttc tcaaacagg ggcaagttg ggccgtttg gccaaacaa ctgctaggt cagggtgac cctcttgat gataagctc caagttgat atgtttatc gagtatttg ttgtcactc aaggatatc aaaggctgg gttttgctc aacttcccc aacacttac gaacaagcg gccctcctt gaggaggct ctgagtggc aaagccgcc ccgaaacaa tactttgac aagtccata aacaaagag agtctcacc agtgttgaa gttgaacaa gttgaagtt gaaggggag gaaatttcc agtagtacc accgcggac caagtcaca ccggaggaa atccctccg gaggaaagt gtgaagaga agtgaggaa aaaccacgt agtagagtg aacattgct gaatatttg aaccagtcg cttccatct ttcgccagt acggataca gggtgtata agcgaggtc acaatgcag accgatttc agttacatt ccggaagtc agagtgaag agactactc cccgatttg atcaacttg gcgtattcc tcggaggag gaaaacgag cctgagact gaaccagac cccaaaaag tgcatctgt gtgtgcaac caagacaat gaagatggt aattttggt gaagaagtt gaagttgaa atgaacgaa gatctatca gaagagaaa aaatcagtc gagtatgat gaggatccc gacgagaag aagagtgaa caaaatcag gactgtgtt tgctatgtg gataaaaca gaagaggtg gttccggtt gaggtccag gaagccaca atccagact gacattttg ggtttaatg cacgagctt ggagcgagt gactctaaa ccgatcgag cgaattgtt gttgaagat atttacgaa gaaaagcgc ttgtcgaag attgttcca aacccgcga cccttggaa aaggaagaa tataacacc gtacttacc gcctttata aaagtcagg cagaaggag gaggccgaa gacgaggat ttcccggag ccttatttc aaagaaatg gaacggaag gatggatta gagaagttt tactgtgac caagggtgt tactacatt ttacagtac gacgagttt aatgttgaa actatcaaa tgcttggcc aagatgatc atcggtgac tataacttg cccttgaaa gactcaatt gagattttt ggggagcac tacaaggag gcactgctt gatgttggc ggccaccaa atcgtaaag aaagggaag aaggagaag aaaaaaaag ggcaaggag gggaaaaag gccaaaccc agcaaaagc aaaagcacg acaaagtca ggaaaaaag aacaaaaag tctgaaaag tcctccaaa aagagcaag aaaagcaaa aagtcggat aaatcctcc aaaaaaagc aagggcaaa aagggtaaa aaaggcaag aaggccaaa agcacgcac gacctcttg gcaccagtg gcccacgaa gacaaagag gtgcaattc ccggcggtt gaggagctc gaggaggag gaactgccc gaaccccgg gctggggag cccgactgg gagtacacg aacctggca atcccgccc gagattgag ctaatccta gccaaccac tgggacaac ttcgaggag aactacatc aaaaacttc aaggacttg ttcttcaac aagcggctg ttgctggag gaaattgtg ccgttcctg gcccgcact cgggagacc atgggcgag tttatagtc cgccctgac caaaaacaa tactacttg agggaattc caaaagacg ctcaacgaa atcggtcta gacttgagg aacgaccaa gagaccaaa accgagctt ttgtgccgg attgaggaa ttccgcgac aaactcctg gggatttgt gacaccaaa atgcaggag tcggaggag gagaggagg cggttcgtg aggcaaaac tggctcttt caccagttt tgccaaatc acgaataac agcgctgag gcgttccag tttgaaatc gaccgaatc gttgacacg ttgcagttc ttgtccgat tattacgtt tccgtgatt acgaaatcc tcaaaggag ggctacgac ttccataag cagatcctg ccgaagttt gagtgtgac gattcgcaa agtgacgac ttcaactcg ctttttatc gacgtggag agcgaaaat tgcgagaaa agtgtgttt catgaatat atcactgtt agtaaccag ggggctttg ggtttcgtc gatagcatg aaactgcta gccttgaag ccggtccac gacatgagg aagttgttt gaagcgggg gagaagggc aaggagaag aagaagaag aaaccaaaa gcgaagcag aaaaaattc gagttggag gaggaggtg atggagagc caggagcgg atttttgag gagtggcaa tgcgcgatt gagggcgaa ctcgcgcgg gcgtacgtc cacttgagt gtcatccgg aacgaggcg ttcaatcgc atcaatgac gcgattagt tggatcaag cgaattttt gaggagatt tacgatact ataggcgaa acgtacgag attagtctc tcgaatgtg aacaaggcg tgcgaattg ttggcgctg tccgtggaa caggagatg ataattcaa ccggagctg gcgttcgaa ggggaacag ttggcccaa atccaggtc gctttgttc cccgacact ttggcggtg gctgaggag ggttacagt gaggggtat ttcaaaatc gagcagctg gagttcatg gtcaaagtt ctcctcgat ttggcacac agtgggtac ataatgaag aactgcttc ctgctgctg atccaggac atcctctac tacgacttt gacgattct gaagacgtt gaaacgccg aaactgtgg cgaaacttg ccaatttcc gccctcaat accttgctg cgtaagctg ttcgatgac gagaagtat ttatattgg agggacttt attatttgt aatttaatg gtcccgctc cctacccgg gaacagttg atgaaagtg cggcaacag ttccggcaa tacgacccg gacttaacc gaaaaagtc accaaggat gcgtttgag tcgattgag ttctggttt gaggacaag cgtgtcaaa agcttgctc ttttcaatg taccaactc gacgaaaat gaagttaac tacactgca cttctgctc gacttttgc aaagataac gacccgata attgggttt gtcaaggct ctggagctg tctctgggc caatttatt tgttgcgac aaagccgtc ggagagaaa ttcgtggcg gaggttctg gaagaccgg attttattc gagaagagg aaaaaagag cacgaggaa aacataatt ctggctaaa agggccgtg agtctccta attgaccga attgtcggt cagtttgag gagcccgaa ggttttagt ttgaaggag gtagaggac acgattgag gaacggtcg ctttcgcgg agtttccag tcggacttt gtcggtcaa ataatcaca gagcacaag ttttccttt acgaatatc gggttcgag gcccgggat gacacagga atggcgttc ttcctgccg cagggcctg gtccagata attgtcaat accgacata acgtggaat tccaaaatc gagctcccc aactcggag agtttcagc gacaaaatc atgaacatt tacgacagt tgccggaac gagaagttc aacaatgtg gtgttagcg cacgagttt ctcaactgt gacgaattg agggaattg ttccaaaac actaataaa ttctcagct cataatccc gtcaaactt gtcaaacgc atagtcact gaagcggaa ctaagcgaa ccggaagag ttcgtcttt caccaggag tcgaagcat tcgctcaac accgacaca acgaaaacg cacagttct tattaa
- >TC005735 RA
aggaagtaa ataaatgag attcaatta acattttgt gaaataaat cgacgtaac cccactttc gtggcgcca aatttgaat gacaacttg tcgcaaaaa tgctgattg aattgatca ggaaatggc tgaaaaaac gcctaggca tccatttga gcatcattc ccaagtact tctacgacg cgacgcggg acggtaccc tcgtggctc aaatcctcc tctactacg acgtcataa ctcctgaag aagcgaacc taattgaag aaacgcaag acccggccc aagccttcg ataactacc aacaccacg tggctaaat ggttgagtc gcatcaaac tgcacctca ccgaaagtg aaatgtacg aaatcgcct actgtgaag gcacagtcg ctgccacaa tcttctacg agttgtact taaaactcg aagccaagg aggactcca gtaaaatta ggcccaaga atcaccatt tccgcatcg agaaagtca ccgagtttc aaatcgacg atgttttta tcgaggcta atcccttgt gcgagcctt tgatcaaac aatgggaca tcatcaact gggaaaagg accacctgg cgagtctca tgatgaaat acatgaact cgcgggaag aataccgca attatttgg aagaaaaga agattgagg aattgagtg cgccggcaa atttggaaa aactggaag acactctta tttttctga aggcggaaa agacggatt atacctacg accagttga tagcccagc aaaaggcag cccttgaaa tggaaccct tccagccaa accaagacg aagccaaac gaatcatgg ccaaaatta agcgacgaa aaaagcaaa gggcccaaa gaaccgcat tcagactcg aactgcaac accaactcc tttcggacg tttggaaga aaatcgaag agagtcaag aactcgaat ttgataaag tcatttgcg aaaaactgt taaaacagt cgtttcacg agaaggaaa tcgaagaaa agctcctct ttatacgga aacagaaag acactattg tggcgaacc gtagagtcc aggacgaaa tcgatcaga agaaacggg acgaggaat tcattcgga gactcttcg aggaagatc tcgaagaac gcttgcggt ttcatgacg agaggatgg ccctcatag ctgagcata agcgcctct acgaggaga aaatgcgac tcaaggcgc tcaagcacc tgaaactgt gccaggaag tgatgggtg atatcgtcg acctttcca taaaaacag cagagtaca aggagatgt acaacatgg agcccaaca aatacgtgc aaagacaat gggcgaatc ttttcataa agcaacaac cgatttttg acttggttg tgccttcgc aagacttga aacagccac acgaggaga tttcggcgg aactggagc aaatttaca ggagggaga ttcagagac aggacatcc tggacgagc aagattttg acgattaca tcaactttc gcaacattt ggcaagttg aggagggac ttgaggaat atctcgctg cgatggagc tcggactta attgtttgg gcttcgccg tccacaaac tcctcctca aaaagtacc ccctgccag taccggaga aagcccaag tgtctccgg aaatcgaga ttgcggttt gtgccactt tggccgact tgacccgga ttgacatgc tccagcgtc tcctcaacc tcagaaata tcaaagtga ttttgatcg aagacgcgg ttaattttt gcatcgaag gctacgaaa gcgaactga agacggagt ttgatgagc cgttccctg aggaagagt acttgcgcc agctgacgc ccaaaaaag gcaaaaaag aggagaaga agaagaaaa agggcaaaa aaggggaca aaaaatcaa agaaaaaag gggacaaag cctcgaaga aaaaaagcg acaaatcct cgaagaaaa gcaagaagg ggaagaaaa aaggggggg agaagacac ccgtggagg tgacgtttg aggagaagc aagcccaaa ccccgcgcc tgttcccgt gggagaaag tggttttct caaacaggg gcaagttgg gccgtttgg ccaaacaac tgctaggtc agggtgacc ctcttgatg ataagctcc aagttgata tgtttatcg agtatttgt tgtcactca aggatatca aaggctggg ttttgctca acttcccca acacttacg aacaagcgg ccctccttg aggaggctc tgagtggca aagccgccc cgaaacaat actttgaca agtccataa acaaagaga gtctcacca gtgttgaag ttgaacaag ttgaagttg aaggggagg aaatttcca gtagtacca ccgcggacc aagtcacac cggaggaaa tccctccgg aggaaagtg tgaagagaa gtgaggaaa aaccacgta gtagagtga acattgctg aatatttga accagtcgc ttccatctt tcgccagta cggatacag ggtgtataa gcgaggtca caatgcaga ccgatttca gttacattc cggaagtca gagtgaaga gactactcc ccgatttga tcaacttgg cgtattcct cggaggagg aaaacgagc ctgagactg aaccagacc ccaaaaagt gcatctgtg tgtgcaacc aagacaatg aagatggta attttggtg aagaagttg aagttgaaa tgaacgaag atctatcag aagagaaaa aatcagtcg agtatgatg aggatcccg acgagaaga agagtgaac aaaatcagg actgtgttt gctatgtgg ataaaacag aagaggtgg ttccggttg aggtccagg aagccacaa tccagactg acattttgg gtttaatgc acgagcttg gagcgagtg actctaaac cgatcgagc gaattgttg ttgaagata tttacgaag aaaagcgct tgtcgaaga ttgttccaa acccgcgac ccttggaaa aggaagaat ataacaccg tacttaccg cctttataa aagtcaggc agaaggagg aggccgaag acgaggatt tcccggagc cttatttca aagaaatgg aacggaagg atggattag agaagtttt actgtgacc aagggtgtt actacattt tacagtacg acgagttta atgttgaaa ctatcaaat gcttggcca agatgatca tcggtgact ataacttgc ccttgaaag actcaattg agatttttg gggagcact acaaggagg cactgcttg atgttggcg gccaccaaa tcgtaaaga aagggaaga aggagaaga aaaaaaagg gcaaggagg ggaaaaagg ccaaaccca gcaaaagca aaagcacga caaagtcag gaaaaaaga acaaaaagt ctgaaaagt cctccaaaa agagcaaga aaagcaaaa agtcggata aatcctcca aaaaaagca agggcaaaa agggtaaaa aaggcaaga aggccaaaa gcacgcacg acctcttgg caccagtgg cccacgaag acaaagagg tgcaattcc cggcggttg aggagctcg aggaggagg aactgcccg aaccccggg ctggggagc ccgactggg agtacacga acctggcaa tcccgcccg agattgagc taatcctag ccaaccact gggacaact tcgaggaga actacatca aaaacttca aggacttgt tcttcaaca agcggctgt tgctggagg aaattgtgc cgttcctgg cccgcactc gggagacca tgggcgagt ttatagtcc gccctgacc aaaaacaat actacttga gggaattcc aaaagacgc tcaacgaaa tcggtctag acttgagga acgaccaag agaccaaaa ccgagcttt tgtgccgga ttgaggaat tccgcgaca aactcctgg ggatttgtg acaccaaaa tgcaggagt cggaggagg agaggaggc ggttcgtga ggcaaaact ggctctttc accagtttt gccaaatca cgaataaca gcgctgagg cgttccagt ttgaaatcg accgaatcg ttgacacgt tgcagttct tgtccgatt attacgttt ccgtgatta cgaaatcct caaaggagg gctacgact tccataagc agatcctgc cgaagtttg agtgtgacg attcgcaaa gtgacgact tcaactcgc tttttatcg acgtggaga gcgaaaatt gcgagaaaa gtgtgtttc atgaatata tcactgtta gtaaccagg gggctttgg gtttcgtcg atagcatga aactgctag ccttgaagc cggtccacg acatgagga agttgtttg aagcggggg agaagggca aggagaaga agaagaaga aaccaaaag cgaagcaga aaaaattcg agttggagg aggaggtga tggagagcc aggagcgga tttttgagg agtggcaat gcgcgattg agggcgaac tcgcgcggg cgtacgtcc acttgagtg tcatccgga acgaggcgt tcaatcgca tcaatgacg cgattagtt ggatcaagc gaatttttg aggagattt acgatacta taggcgaaa cgtacgaga ttagtctct cgaatgtga acaaggcgt gcgaattgt tggcgctgt ccgtggaac aggagatga taattcaac cggagctgg cgttcgaag gggaacagt tggcccaaa tccaggtcg ctttgttcc ccgacactt tggcggtgg ctgaggagg gttacagtg aggggtatt tcaaaatcg agcagctgg agttcatgg tcaaagttc tcctcgatt tggcacaca gtgggtaca taatgaaga actgcttcc tgctgctga tccaggaca tcctctact acgactttg acgattctg aagacgttg aaacgccga aactgtggc gaaacttgc caatttccg ccctcaata ccttgctgc gtaagctgt tcgatgacg agaagtatt tatattgga gggacttta ttatttgta atttaatgg tcccgctcc ctacccggg aacagttga tgaaagtgc ggcaacagt tccggcaat acgacccgg acttaaccg aaaaagtca ccaaggatg cgtttgagt cgattgagt tctggtttg aggacaagc gtgtcaaaa gcttgctct tttcaatgt accaactcg acgaaaatg aagttaact acactgcac ttctgctcg acttttgca aagataacg acccgataa ttgggtttg tcaaggctc tggagctgt ctctgggcc aatttattt gttgcgaca aagccgtcg gagagaaat tcgtggcgg aggttctgg aagaccgga ttttattcg agaagagga aaaaagagc acgaggaaa acataattc tggctaaaa gggccgtga gtctcctaa ttgaccgaa ttgtcggtc agtttgagg agcccgaag gttttagtt tgaaggagg tagaggaca cgattgagg aacggtcgc tttcgcgga gtttccagt cggactttg tcggtcaaa taatcacag agcacaagt tttccttta cgaatatcg ggttcgagg cccgggatg acacaggaa tggcgttct tcctgccgc agggcctgg tccagataa ttgtcaata ccgacataa cgtggaatt ccaaaatcg agctcccca actcggaga gtttcagcg acaaaatca tgaacattt acgacagtt gccggaacg agaagttca acaatgtgg tgttagcgc acgagtttc tcaactgtg acgaattga gggaattgt tccaaaaca ctaataaat tctcagctc ataatcccg tcaaacttg tcaaacgca tagtcactg aagcggaac taagcgaac cggaagagt tcgtctttc accaggagt cgaagcatt cgctcaaca ccgacacaa cgaaaacgc acagttctt attaaaaat ctttatttt tctcatata t
