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Gene information
General information
TC004708 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 956287..973525
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004708
      iBeetle screen iB_06183

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004708 RA
        MVLSANKIN YKGETGINT NYYATRDNT 
        NCRQSKSPQ TYSDSWYVR ETTTYGSSS 
        AATVDKEQR HSCWCWSKS AGAAEPAMR 
        YTNGPQSAA SMVASYGVY NDATLRADE 
        DEDGFIPRA NVYPGVVVN AAGDSSVLR 
        RGQEAAPAE SYSCGWCFG SRSTTTTPS 
        NRNSCLISW CRPTILLLI LVLLVVVFV 
        LVSGILLYF NCTLKCFYV CMFTQSFDV 
        CL
      CDS sequence
      • >TC004708 RA
        atggttttg agcgctaac aaaattaat tacaaagga 
        gaaacggga ataaatacg aattactac gcgacacgt 
        gacaatacg aattgccgt cagtctaaa tcgccccag 
        acgtatagt gactcttgg tacgtaagg gaaactacc 
        acatatggc agcagttcg gccgcgacg gtcgataaa 
        gaacagaga cattcctgc tggtgctgg tcgaagtct 
        gcaggagcg gctgaaccc gccatgagg tacacgaac 
        ggcccccag tcggcggcg agcatggtg gcctcctac 
        ggcgtctac aacgacgcc acgctccgg gccgacgag 
        gacgaggac ggcttcatc cccagggcg aacgtgtat 
        cccggcgtt gtggtgaac gcagcgggc gatagtagc 
        gtcctgcga cgagggcag gaagcggct cctgctgaa 
        tcatactcg tgtggttgg tgcttcggc tcgaggtcg 
        acgaccacc actccttcg aacaggaac tcctgctta 
        atctcctgg tgtcggccc actatcctc ctgctcatt 
        ttagtctta ctcgttgtg gttttcgta ttggtctcc 
        gggattcta ctatacttc aactgtacg ttgaaatgt 
        ttttatgtc tgcatgttt actcaaagt ttcgatgtt 
        tgcttgtag
      mRNA sequence
      • >TC004708 RA
        gcgagcttg caacccgaa aggacgtga gcttttagc 
        aggatatcg ttcgcgcgc gcttaccac gtgtttcgt 
        gatattgag aaaaatgac ttgctaaca ctcgcagct 
        tttcggctg gaaattttt tatttgcgc ggtttggag 
        gcacgaatc gggggtttt cagatgctg gaaaaggaa 
        tggcgctaa gcaaaaact accggtcat gtgcttaaa 
        aacctttaa tttgttgtt gtacgacaa aaatgttgc 
        gttacatac gtttacgtt tgtgatgtc ttcgcggcg 
        ataaaacaa aagtgaaaa atgtgtgaa gaatggttt 
        tgagcgcta acaaaatta attacaaag gagaaacgg 
        gaataaata cgaattact acgcgacac gtgacaata 
        cgaattgcc gtcagtcta aatcgcccc agacgtata 
        gtgactctt ggtacgtaa gggaaacta ccacatatg 
        gcagcagtt cggccgcga cggtcgata aagaacaga 
        gacattcct gctggtgct ggtcgaagt ctgcaggag 
        cggctgaac ccgccatga ggtacacga acggccccc 
        agtcggcgg cgagcatgg tggcctcct acggcgtct 
        acaacgacg ccacgctcc gggccgacg aggacgagg 
        acggcttca tccccaggg cgaacgtgt atcccggcg 
        ttgtggtga acgcagcgg gcgatagta gcgtcctgc 
        gacgagggc aggaagcgg ctcctgctg aatcatact 
        cgtgtggtt ggtgcttcg gctcgaggt cgacgacca 
        ccactcctt cgaacagga actcctgct taatctcct 
        ggtgtcggc ccactatcc tcctgctca ttttagtct 
        tactcgttg tggttttcg tattggtct ccgggattc 
        tactatact tcaactgta cgttgaaat gtttttatg 
        tctgcatgt ttactcaaa gtttcgatg tttgcttgt 
        agtttgcac atttgccag aacatgcgc tcgcaacta 
        gaccaaaca taacttcca cactaccta catttattt 
        atgatattg tactttcac ttaaatcgg ccacataca 
        acacaacat tgattttta tttcaataa cttacgaac 
        agaacgacc gcatggaaa ctagacggc ccggctgct 
        tgaaaattc gcagatata agagcgaaa caggttgca 
        catttaaaa aaagacaca cggtgttat tttttttaa 
        ttctgttaa acatgcaac gaactaaat aaaacactt 
        tcaacaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY