Status
Please wait ...
Gene information
General information
TC004600 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6827774..6835773
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004600
    iBeetle screen iB_06181

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004600 RA
      MSEGESKKL SELRVVDLK AELEKRGLD QKGIKRDLV 
      DRLQKALQS EGLDPETYC FEIGEKKPV KRLSTSSSS 
      YQGDDNVCT EKTETDSDA KEEETNVVT KEETEEQVG 
      SGSNKTDAE PETINLTLE EEENIPEES AERESKNSG 
      DKIVSDEKP TTPKQVQME ESTSPTSQQ ASKKTPETP 
      GTSPSNGAE EPSQKKAQS NPRLLWITN VPKNTRANE 
      LKQLLSSCG KVVCAKVVV NARFPGSCC FGYITMGSA 
      EDAANVISK LNNTEFNGQ IIKIDKFEN IKSDINPTR 
      KTKSEENKK SQTSEKKED KTKKSPEGK EKNEEKNDD 
      NEKAAKSPA SPERRKSLN SDRSSRGRR RTTSKERQK 
      ERPMHRSTS RGHKSSVLT FDKIKEERE RQRMRERER 
      AIREESRRR REEAERMRE MREITRRQK SEADRLERE 
      REKLRIERD RIEREKSEL IKLERERQR IEREKLEME 
      KMELQRSRL RLEEDRRAV KRPLPVPYR REDTFEERK 
      RPNNVRHFE DTTPRFEPP PPPTPVQQK KFAPSKDFK 
      RMSYDKHES FSDKRRDYN SDRSHPGSS VRSNPSVNR 
      YDNVRGRDS RDAPSRDSQ SNLMRSKER YNERDRSPH 
      FRPLGESRD RHSSSQGKS DRDHHYSEQ NSDRWNHPT 
      NSQSKFGSN NQKPWSGKS NWRPDPPTV DRWNSSRSS 
      MNQQFSGNS NMAPTCPPP PGINNYSDR FDYKSSGLR 
      KY
    CDS sequence
    • >TC004600 RA
      atgtctgaa ggcgagagc aagaaattg tcggaattg 
      cgtgtagtg gatttaaaa gccgagttg gaaaaacgg 
      ggtttggac caaaaagga atcaaacga gatctggtg 
      gaccgcctt caaaaggca ctgcaaagc gaggggttg 
      gaccccgaa acgtactgc tttgaaatc ggggagaag 
      aaaccagtt aagaggctt tcaacttcg tcatcttct 
      taccaaggg gatgacaat gtttgtact gaaaagact 
      gaaactgat agcgacgca aaggaggaa gagacaaat 
      gttgtcacg aaagaggaa acagaggaa caagttggt 
      agtggatcg aataagact gatgcggag ccagaaacc 
      ataaattta actttggaa gaggaggaa aatattccc 
      gaagagagc gccgagcgg gagagtaag aattctggc 
      gataaaata gtttccgat gagaagcca acaacacct 
      aaacaagtc cagatggaa gagtcgacg tcacccact 
      tcacagcag gcctccaag aaaacgcca gaaacacca 
      gggactagc cctagcaat ggagcggaa gaaccgagt 
      cagaagaag gcccagtca aaccctcgc ctactctgg 
      attactaac gtccctaaa aacacgcgc gctaacgaa 
      ctcaaacaa ctgctttcg tcttgtgga aaagtcgtt 
      tgcgctaaa gtcgtcgtt aacgcaagg tttcccgga 
      tcatgttgc ttcggatac atcacaatg ggatcggct 
      gaggatgcg gctaatgtc atttccaaa ctaaataat 
      accgaattt aacggacag attatcaag attgataaa 
      tttgagaat atcaaatcg gatattaat ccgacacgg 
      aaaactaaa agcgaagaa aataagaaa tcacaaacg 
      agtgaaaag aaggaggat aaaactaag aaaagtcca 
      gagggcaaa gaaaagaat gaagagaaa aatgatgat 
      aatgagaaa gcggcgaaa agtccagcc agccctgaa 
      agaaggaag tccttaaat tccgaccga tctagtcgc 
      ggacgcaga cgtaccacc tcaaaagag cggcagaaa 
      gaaaggccg atgcatcgc tccacctct cgagggcat 
      aagtccagt gtcctaact ttcgacaaa attaaagag 
      gaacgagag cggcagcgc atgcgcgag cgcgaacgc 
      gccattcgc gaagagagc cgacgccgt cgagaggag 
      gcagagcgc atgcgcgaa atgcgtgaa atcacccgt 
      cgccagaaa agcgaagca gaccgatta gaacgcgaa 
      cgcgagaaa ttgcgaatc gaacgcgac cgaatcgag 
      cgcgaaaaa agcgaattg ataaaactg gagcgagag 
      cgtcaacga atcgaacgc gagaagcta gaaatggaa 
      aaaatggag ttacaaagg tcacgtttg aggcttgag 
      gaggaccgc agggctgtg aaacgtcca cttcctgtg 
      ccctacaga cgggaggac acattcgag gagcgaaaa 
      cgtccaaac aatgtgcgt cattttgag gacacaacg 
      ccgcggttt gaaccgccg ccgccgcct actccggtg 
      caacagaag aagtttgca ccttcgaaa gattttaaa 
      cgaatgtcc tatgataag catgagtcg ttttcggac 
      aaacgcagg gattataat tcggacagg tcgcatcca 
      gggtcgtcg gttaggtcg aacccttcg gttaatcgg 
      tatgataac gtcagaggg cgcgattcg cgggatgcg 
      ccatctagg gactcgcaa tcgaacttg atgaggtcg 
      aaggagcgg tacaatgag agggacagg agcccccat 
      tttaggccc cttggggag agcagggac cgccatagt 
      tcgtctcag gggaaatca gatcgagat catcactac 
      tcagaacaa aattctgat cgttggaac cacccaact 
      aactcacaa tcgaagttc ggttcaaat aaccaaaag 
      ccttggtct ggcaaaagc aactggcgt ccagaccca 
      cctacggtc gacagatgg aactcaagt aggtcttca 
      atgaatcaa cagttctct ggaaattcc aatatggcg 
      ccgacttgt ccacctccg ccaggaatt aataattat 
      tcagatcga ttcgactac aagtccagt ggacttaga 
      aaatattaa
    mRNA sequence
    • >TC004600 RA
      tgcaaccta aatggctgg tgtctgtgt gaaaaatta 
      gcatttttt ctaaattta ttgaaatgt ctgaaggcg 
      agagcaaga aattgtcgg aattgcgtg tagtggatt 
      taaaagccg agttggaaa aacggggtt tggaccaaa 
      aaggaatca aacgagatc tggtggacc gccttcaaa 
      aggcactgc aaagcgagg ggttggacc ccgaaacgt 
      actgctttg aaatcgggg agaagaaac cagttaaga 
      ggctttcaa cttcgtcat cttcttacc aaggggatg 
      acaatgttt gtactgaaa agactgaaa ctgatagcg 
      acgcaaagg aggaagaga caaatgttg tcacgaaag 
      aggaaacag aggaacaag ttggtagtg gatcgaata 
      agactgatg cggagccag aaaccataa atttaactt 
      tggaagagg aggaaaata ttcccgaag agagcgccg 
      agcgggaga gtaagaatt ctggcgata aaatagttt 
      ccgatgaga agccaacaa cacctaaac aagtccaga 
      tggaagagt cgacgtcac ccacttcac agcaggcct 
      ccaagaaaa cgccagaaa caccaggga ctagcccta 
      gcaatggag cggaagaac cgagtcaga agaaggccc 
      agtcaaacc ctcgcctac tctggatta ctaacgtcc 
      ctaaaaaca cgcgcgcta acgaactca aacaactgc 
      tttcgtctt gtggaaaag tcgtttgcg ctaaagtcg 
      tcgttaacg caaggtttc ccggatcat gttgcttcg 
      gatacatca caatgggat cggctgagg atgcggcta 
      atgtcattt ccaaactaa ataataccg aatttaacg 
      gacagatta tcaagattg ataaatttg agaatatca 
      aatcggata ttaatccga cacggaaaa ctaaaagcg 
      aagaaaata agaaatcac aaacgagtg aaaagaagg 
      aggataaaa ctaagaaaa gtccagagg gcaaagaaa 
      agaatgaag agaaaaatg atgataatg agaaagcgg 
      cgaaaagtc cagccagcc ctgaaagaa ggaagtcct 
      taaattccg accgatcta gtcgcggac gcagacgta 
      ccacctcaa aagagcggc agaaagaaa ggccgatgc 
      atcgctcca cctctcgag ggcataagt ccagtgtcc 
      taactttcg acaaaatta aagaggaac gagagcggc 
      agcgcatgc gcgagcgcg aacgcgcca ttcgcgaag 
      agagccgac gccgtcgag aggaggcag agcgcatgc 
      gcgaaatgc gtgaaatca cccgtcgcc agaaaagcg 
      aagcagacc gattagaac gcgaacgcg agaaattgc 
      gaatcgaac gcgaccgaa tcgagcgcg aaaaaagcg 
      aattgataa aactggagc gagagcgtc aacgaatcg 
      aacgcgaga agctagaaa tggaaaaaa tggagttac 
      aaaggtcac gtttgaggc ttgaggagg accgcaggg 
      ctgtgaaac gtccacttc ctgtgccct acagacggg 
      aggacacat tcgaggagc gaaaacgtc caaacaatg 
      tgcgtcatt ttgaggaca caacgccgc ggtttgaac 
      cgccgccgc cgcctactc cggtgcaac agaagaagt 
      ttgcacctt cgaaagatt ttaaacgaa tgtcctatg 
      ataagcatg agtcgtttt cggacaaac gcagggatt 
      ataattcgg acaggtcgc atccagggt cgtcggtta 
      ggtcgaacc cttcggtta atcggtatg ataacgtca 
      gagggcgcg attcgcggg atgcgccat ctagggact 
      cgcaatcga acttgatga ggtcgaagg agcggtaca 
      atgagaggg acaggagcc cccatttta ggccccttg 
      gggagagca gggaccgcc atagttcgt ctcagggga 
      aatcagatc gagatcatc actactcag aacaaaatt 
      ctgatcgtt ggaaccacc caactaact cacaatcga 
      agttcggtt caaataacc aaaagcctt ggtctggca 
      aaagcaact ggcgtccag acccaccta cggtcgaca 
      gatggaact caagtaggt cttcaatga atcaacagt 
      tctctggaa attccaata tggcgccga cttgtccac 
      ctccgccag gaattaata attattcag atcgattcg 
      actacaagt ccagtggac ttagaaaat attaatttt 
      gtatttatt agccacctg ttttttttg tttgttaca 
      attaattat gtgtttgac caatttaat aaataatat 
      ttaaagtaa cacgctttt tgtttcatt tatttttta 
      cattagaat tacaacaat caacaataa aaataacaa 
      gacacgaaa ttaatccaa gccgtgttc tacaacgga 
      cagcattga acttgtttt agacggagc caaaaggta 
      cgatttttt gtccattcg gaacggtac caaaacctt 
      gactccgtc caactgacc gcttgattc gagcttcca 
      acctgcgat aaactggaa cgaaaagat gaaatattt 
      gttgttttg cgtcacgac gcttaaata ggcatcgct 
      aatcccaaa acctgaaac aaaaaatag tgaagtcag 
      gctaggacc actttagaa acataacaa gagaaataa 
      agacgtttg tctgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY