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Gene information
General information
TC004552 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2662163..2663597
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004552
      iBeetle screen iB_06180

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004552 RA
        MPFADESEK KKLYEFDSS YLTELNQIR TQLNLVTIS 
        DDEEDPTSD VQVPPSPKF TFNEELYDC NTDYMKELN 
        DIRKQLNLK EIVEGGSSS KESAPKIET KTLFINNGA 
        VRRKKKPTK VVQLDANYT PPKKKKRSQ DSSPSENSS 
        PVRENPYIF PDFAQVKTN FCSLNVPTC DSPEEKPKS 
        KVTILVDEE LQSLRSHNT CLTTGLDIP RISNNVHTF 
        PSIVHIDKW ATIFTHEAK LEIFSPKSC DSKRVRKKV 
        SLGESKAIE TFPQMHGDQ KIENKMLEV ETISSLNNM 
        YHVKRKSND VSHVETVTT SADDSKSES GEEVKVGCR 
        CFSICFNKR SPNFFS
      CDS sequence
      • >TC004552 RA
        atgcctttc gccgacgaa agtgaaaag aaaaagttg 
        tacgaattc gatagcagc tacttaaca gagttaaat 
        cagatcaga acgcagttg aatcttgtg acgatttct 
        gatgacgaa gaagaccca actagtgac gtacaagtg 
        cctccctca ccgaaattc accttcaat gaggagctg 
        tacgactgt aataccgac tacatgaaa gaactgaac 
        gacataagg aagcaactg aatctcaaa gaaatcgta 
        gaaggtggt agtagtagt aaagaaagt gcgccaaaa 
        atcgaaacg aaaacactt tttatcaac aatggtgct 
        gttagacgc aagaaaaag ccgacaaaa gtggtgcaa 
        ttggacgcg aattacaca ccaccgaag aaaaaaaag 
        cgaagccaa gattctagt cctagtgaa aattcgtca 
        ccagtccgg gaaaatccg tacatattt ccagatttc 
        gcgcaggtg aaaactaac ttttgttcg ttaaacgtc 
        ccgacttgt gattcccct gaagaaaaa cccaaatcc 
        aaggtgaca atccttgtt gacgaggag ctccagtct 
        ctgcggagt cacaacacc tgcttaaca acaggactg 
        gacattcct cgaatatca aataatgtt catactttt 
        ccgtccatc gtgcacatc gacaagtgg gcaactatt 
        ttcactcac gaagccaaa ctggagatt ttttcgccc 
        aaatcctgt gacagtaaa agagttagg aagaaagtg 
        agtttaggc gaatcgaaa gcgattgag actttccct 
        caaatgcac ggtgatcag aaaatcgaa aataagatg 
        ctggaagtc gagacgatt tctagtctc aataatatg 
        tatcatgtt aaaaggaaa agtaatgat gtaagccat 
        gtggagacg gtcactacg agcgctgat gattcgaag 
        agtgaaagt ggtgaagaa gtaaaggtc ggatgtcgc 
        tgtttttct atttgtttc aataagagg tcaccgaat 
        tttttttcc tag
      mRNA sequence
      • >TC004552 RA
        ttagactaa taacgttct cccactaat ttattagca 
        taatctcaa gacaaacaa tttgtggaa gtttaatga 
        aggcctcgc ctgtttgtt tgtgagcaa ttttgaata 
        tttttgaca ttgtgacaa gtgacatat tgacatatg 
        agtaaatta gtgccacaa aaacggcca ttatatgcc 
        tttcgccga cgaaagtga aaagaaaaa gttgtacga 
        attcgatag cagctactt aacagagtt aaatcagat 
        cagaacgca gttgaatct tgtgacgat ttctgatga 
        cgaagaaga cccaactag tgacgtaca agtgcctcc 
        ctcaccgaa attcacctt caatgagga gctgtacga 
        ctgtaatac cgactacat gaaagaact gaacgacat 
        aaggaagca actgaatct caaagaaat cgtagaagg 
        tggtagtag tagtaaaga aagtgcgcc aaaaatcga 
        aacgaaaac actttttat caacaatgg tgctgttag 
        acgcaagaa aaagccgac aaaagtggt gcaattgga 
        cgcgaatta cacaccacc gaagaaaaa aaagcgaag 
        ccaagattc tagtcctag tgaaaattc gtcaccagt 
        ccgggaaaa tccgtacat atttccaga tttcgcgca 
        ggtgaaaac taacttttg ttcgttaaa cgtcccgac 
        ttgtgattc ccctgaaga aaaacccaa atccaaggt 
        gacaatcct tgttgacga ggagctcca gtctctgcg 
        gagtcacaa cacctgctt aacaacagg actggacat 
        tcctcgaat atcaaataa tgttcatac ttttccgtc 
        catcgtgca catcgacaa gtgggcaac tattttcac 
        tcacgaagc caaactgga gattttttc gcccaaatc 
        ctgtgacag taaaagagt taggaagaa agtgagttt 
        aggcgaatc gaaagcgat tgagacttt ccctcaaat 
        gcacggtga tcagaaaat cgaaaataa gatgctgga 
        agtcgagac gatttctag tctcaataa tatgtatca 
        tgttaaaag gaaaagtaa tgatgtaag ccatgtgga 
        gacggtcac tacgagcgc tgatgattc gaagagtga 
        aagtggtga agaagtaaa ggtcggatg tcgctgttt 
        ttctatttg tttcaataa gaggtcacc gaatttttt 
        ttcctaggg taattttaa gaataatcg gtttaacta 
        gactaagct tatatctaa atgccactg cttcggaag 
        taaactttg agactgctt taacactgg ttttatttt 
        actttaatc tgatcaaag tggactgtg gatttggaa 
        ctaaggtgg caacattgt gcaagatga tctgggact 
        atttcagtt aatgtagcc aacttaatt acagaatca 
        cgggctccg ttaataaaa aactctcag caag
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY